1.一種大麥花藥誘導(dǎo)培養(yǎng)基,該培養(yǎng)基的配方如下:
KNO3 900~1100mg/L,NH4NO3 900~1100mg/L,谷氨酰胺 500~700mg/L,Ca(NO3)2?4H2O 330~360mg/L,KH2PO4 270~330mg/L,KCl 60~70mg/L,MgSO4?7H2O 30~40mg/L,Na2-EDTA 35~40mg/L,F(xiàn)eSO4?7H2O 26~30mg/L,MnSO4?4H2O 4.0~5.0mg/L,ZnSO4?7H2O 1.2~1.8mg/L,H3BO3 1.4~1.8mg/L,KI 0.7~0.9mg/L,甲基磺酸乙酯 0.35~0.45mg/L,肌醇 90~110mg/L,甘氨酸 1.8~2.2mg/L,丙氨酸 1.4~1.6mg/L,葉酸 0.25~0.35mg/L,維生素B1 0.09~0.11mg/L,維生素B6 0.09~0.11mg/L,煙酸 0.45~0.55mg/L,蔗糖 45~55g/L,瓊脂 7~8g/L;
2,4-D 1.8~2.2mg/L,NAA 0.45~0.55mg/L,復(fù)酞核酸0.45~0.55mg/L,水解蛋白 0.25~0.35g/L,植物硫化激動(dòng)素PSK-α 23~27pmol/L,胰島素 3.5~4.5mg/L,生物素 0.18~0.22mg/L,PH 5.6~6.0。