本發(fā)明涉及基因技術(shù)領(lǐng)域的核酸及其引物和檢測(cè)方法，具體涉及一種基于rs737865的檢測(cè)利培酮用藥效果的試劑盒。
背景技術(shù)：
利培酮(risperidone)分子式為c23h27fn4o2。利培酮為苯并異惡唑衍生物，是新一代的抗精神病藥。其活性成份利培酮是一種具有獨(dú)特性質(zhì)的選擇性單胺能拮抗劑，它與5-羥色胺能的5-ht2受體和多巴胺的d2受體有很高的親和力。利培酮也能與腎上腺素能受體結(jié)合，并且以較低的親和力與h1-組胺能受體和α2-腎上腺素受體結(jié)合。利培酮不與膽堿能受體結(jié)合。利培酮是強(qiáng)有力的d2拮抗劑，可以改善精神分裂癥的陽(yáng)性癥狀，但它引起的運(yùn)動(dòng)功能抑制，以及強(qiáng)直性昏厥都要比經(jīng)典的抗精神病藥少。對(duì)中樞系統(tǒng)的5-羥色胺和多巴胺拮抗作用的平衡可以減少發(fā)生錐體外系副作用的可能，并將其治療作用擴(kuò)展到精神分裂癥的陰性癥狀和情感癥狀。研究表明，利培酮的藥效和藥物副作用有明顯的個(gè)體差異，原因是其代謝受到遺傳因素及環(huán)境因素的影響，但遺傳因素是最主要的原因。因此開(kāi)發(fā)更多的與利培酮代謝相關(guān)的分子標(biāo)記物從而對(duì)患者實(shí)現(xiàn)個(gè)體化治療是非常有必要的。
comt(catechol-o-methyltransferase)基因位于人類(lèi)第22號(hào)染色體q11.21上，在不同的細(xì)胞中被翻譯成不同長(zhǎng)度的蛋白。在大腦的神經(jīng)細(xì)胞中，comt基因表達(dá)為膜結(jié)合的兒茶酚-o-甲基轉(zhuǎn)移酶蛋白；大腦中的兒茶酚-o-甲基轉(zhuǎn)移酶蛋白主要分布在前額葉皮質(zhì)區(qū)，它可以保證神經(jīng)遞質(zhì)如多巴胺等在一個(gè)穩(wěn)定的水平上。在肝、腎及血液等器官中表達(dá)為可溶性的兒茶酚-o-甲基轉(zhuǎn)移酶蛋白，它可以催化甲基從s-腺苷甲硫氨酸轉(zhuǎn)移到兒茶酚胺上，此類(lèi)物質(zhì)包括神經(jīng)遞質(zhì)多巴胺、腎上腺素、去甲腎上腺素等。comt基因在代謝治療高血壓、哮喘、帕金森疾病的兒茶酚藥物方面起著重要的作用。由于利培酮與多巴胺、5-羥色胺等多種神經(jīng)遞質(zhì)受體均有相互作用，因此尋找利培酮代謝與comt基因多態(tài)性的關(guān)系是現(xiàn)有技術(shù)急需解決的難題。
對(duì)現(xiàn)有文獻(xiàn)的檢索，未發(fā)現(xiàn)有本發(fā)明的comt基因snprs737865為利培酮分子標(biāo)記物的相關(guān)報(bào)道。現(xiàn)有的利培酮分子標(biāo)記研究多基于歐美人群，不能在中國(guó)漢族人群中應(yīng)用。而且由于精神分裂病人利培酮單藥治療后符合標(biāo)準(zhǔn)的病人較少，樣本收集十分困難，現(xiàn)有研究由于樣本量小，不具有相應(yīng)的代表性。而本發(fā)明通過(guò)擴(kuò)大樣本量在中國(guó)漢族人群中進(jìn)行大規(guī)模發(fā)掘和驗(yàn)證，發(fā)現(xiàn)了comt基因snprs737865可以作為利培酮分子標(biāo)記物，具有明顯的經(jīng)濟(jì)效益。
技術(shù)實(shí)現(xiàn)要素：
針對(duì)現(xiàn)有技術(shù)中的缺陷，本發(fā)明的目的是提供一種基于rs737865的檢測(cè)利培酮用藥效果的試劑盒；具體地講是一種分離核酸、一種等位基因特異性的核酸引物和comt基因snprs737865單核苷酸多態(tài)性的檢測(cè)方法。
本發(fā)明的目的是通過(guò)以下技術(shù)方案實(shí)現(xiàn)的：
第一方面，本發(fā)明提供了一種分離的含單核苷酸多態(tài)性位點(diǎn)的核苷酸，所述核苷酸的序列如seqidno：1所示，所述單核苷酸多態(tài)性位點(diǎn)位于第858位，rs737865為a→g。
第二方面，本發(fā)明提供了一種如前述的核苷酸在制備檢測(cè)利培酮用藥效果的試劑盒中的用途。
優(yōu)選的，snprs737865為利培酮分子標(biāo)記物。
第三方面，本發(fā)明提供了一種分離的核苷酸，所述核苷酸的序列如seqidno：1所示第858位，該位點(diǎn)為a。
第四方面，本發(fā)明提供了一種檢測(cè)利培酮用藥效果的試劑盒，所述試劑盒包括：根據(jù)如seqidno：1所示序列的第858位的上下游設(shè)計(jì)的引物對(duì)。
作為優(yōu)選方案，所述引物對(duì)為野生型引物對(duì)或突變型引物對(duì)，其中，所述野生型引物對(duì)的序列如seqidno：3、seqidno：4所示：
正向引物：5′-acaataaaaagcaacaggacacaaaaaacc-3′seqidno：3
反向引物：5′-tgctgtgatgaggggctccagatag-3′seqidno：4；
所述突變型引物對(duì)的序列如seqidno：5、seqidno：4所示：
正向引物：5′-aaaaagcaacaggacacaaaaagcc-3′seqidno：5
反向引物：5′-tgctgtgatgaggggctccagatag-3′seqidno：4。
第五方面，本發(fā)明提供了一種如前述的試劑盒的非診斷目的使用方法，其包括如下步驟：
s1，提取樣品dna；
s2，利用所述引物對(duì)進(jìn)行擴(kuò)增，得擴(kuò)增產(chǎn)物；
s3，檢測(cè)樣品中是否存在如seqidno：1所示的單核苷酸多態(tài)性位點(diǎn)rs737865。
作為優(yōu)選方案，所述步驟s3采用dna測(cè)序、雜交測(cè)序、酶促錯(cuò)配切割、異源雙鏈分析、點(diǎn)雜交、寡核苷酸陣列、mini-sequencing、分子信標(biāo)中的任意一種方法。
作為優(yōu)選方案，步驟s3包括如下步驟：采用abi7700熒光定量pcr儀，對(duì)所取樣品的位點(diǎn)rs737865進(jìn)行分型，檢測(cè)第858位否存在單核苷酸多態(tài)性。
作為優(yōu)選方案，所述引物對(duì)的長(zhǎng)度為15-50bp。
作為優(yōu)選方案，步驟s2中，所述擴(kuò)增的具體條件如下：反應(yīng)體系總體積為20μl，其中：
100μmol·l-1dntps；
50mmol·l-1kcl；
10mmol·l-1tris-hcl，ph為8.3；
1.5mmol·l-1mgcl2：
0.5utaqdna聚合酶；
20-50ng模板dna；
0.5μl10pmseqidno.3；
0.5μl10pmseqidno.4；
雙蒸水補(bǔ)足至20ul；
pcr反應(yīng)條件：95℃2mins；30×(94℃30s；58℃30s，72℃30s)72℃5mins。
第六方面，本發(fā)明提供了一種用于檢測(cè)如前述的核苷酸的snp位點(diǎn)的野生型引物對(duì)，所述引物對(duì)的序列如seqidno：3、seqidno：4所示：
正向引物：5′-acaataaaaagcaacaggacacaaaaaacc-3′seqidno：3
反向引物：5′-tgctgtgatgaggggctccagatag-3′seqidno：4。
第七方面，本發(fā)明提供了一種用于檢測(cè)如前述的核苷酸的snp位點(diǎn)的突變型引物對(duì)，所述引物對(duì)的序列如seqidno：5、seqidno：4所示：
正向引物：5′-aaaaagcaacaggacacaaaaagcc-3′seqidno：5
反向引物：5′-tgctgtgatgaggggctccagatag-3′seqidno：4。
本發(fā)明采用的樣品來(lái)自上海市精神衛(wèi)生中心的995個(gè)精神分裂癥患者，年齡18～60歲。全體參與者均由家屬在正式的知情同意書(shū)上同意簽字。
在上述的基礎(chǔ)上，本發(fā)明采用片段長(zhǎng)度差異等位基因特異性pcr(fldas-pcr)分型技術(shù)對(duì)comt基因snprs737865位點(diǎn)及其頻率分布情況進(jìn)行了檢測(cè)。已經(jīng)發(fā)現(xiàn)了comt基因snprs737865第858位a→g多態(tài)性。
與現(xiàn)有技術(shù)相比，本發(fā)明具有如下的有益效果：
本發(fā)明為研究我國(guó)人群中comt基因多態(tài)性與臨床用藥安全的關(guān)系奠定了基礎(chǔ)，為臨床用藥個(gè)體化提供理論基礎(chǔ)，同時(shí)也為基于藥物基因組學(xué)理念的新藥研發(fā)提供指導(dǎo)依據(jù)。
具體實(shí)施方式
下面結(jié)合具體實(shí)施例對(duì)本發(fā)明進(jìn)行詳細(xì)說(shuō)明。以下實(shí)施例將有助于本領(lǐng)域的技術(shù)人員進(jìn)一步理解本發(fā)明，但不以任何形式限制本發(fā)明。應(yīng)當(dāng)指出的是，對(duì)本領(lǐng)域的普通技術(shù)人員來(lái)說(shuō)，在不脫離本發(fā)明構(gòu)思的前提下，還可以做出若干變形和改進(jìn)。這些都屬于本發(fā)明的保護(hù)范圍。
下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常照常規(guī)條件，例如sambrook等人的分子克?。簩?shí)驗(yàn)室手冊(cè)(newyork：coldspringharborlaboratorypress，1989)中所述的條件，或按照制造廠(chǎng)商所建議的條件。
本發(fā)明涉及comt基因第二號(hào)內(nèi)含子snprs737865的單核苷酸多態(tài)性的檢測(cè)方法，同時(shí)涉及相關(guān)的分離核酸以及引物。本發(fā)明通過(guò)關(guān)聯(lián)分析發(fā)現(xiàn)了comt基因第二號(hào)內(nèi)含子snprs737865能夠作為利培酮的分子標(biāo)記物，該comt基因第二號(hào)內(nèi)含子的序列如seqidno：1所示，其中第858位存在單核苷酸多態(tài)性，即snprs737865。本發(fā)明針對(duì)該snprs737865設(shè)計(jì)了相關(guān)的引物對(duì)。
本發(fā)明采用fldas—pcr探針技術(shù)對(duì)comt基因的第二號(hào)內(nèi)含子snp位點(diǎn)及其頻率分布情況進(jìn)行了檢測(cè)，發(fā)現(xiàn)comt基因的第二號(hào)內(nèi)含子第858位a→g多態(tài)性。
具體而言，本發(fā)明在comt基因第二號(hào)內(nèi)含子上的snprs737865上下游設(shè)計(jì)fldas-pcr引物，然后用聚丙烯酰胺凝膠電泳對(duì)snp位點(diǎn)rs737865進(jìn)行分型，發(fā)現(xiàn)comt基因第二號(hào)內(nèi)含子snp(即第858位a→g多態(tài)性)。
本發(fā)明有大量的分析技術(shù)可用于檢測(cè)comt基因第二號(hào)內(nèi)含子中所述位點(diǎn)是否存在單核苷酸多態(tài)性；這些技術(shù)包括(但并不限于)：dna測(cè)序、雜交測(cè)序；酶促錯(cuò)配切割、異源雙鏈分析、點(diǎn)雜交、寡核苷酸陣列(dna芯片)、mini-sequencing、分子信標(biāo)等，變性高效液相色譜法(dhplc)。本發(fā)明的檢測(cè)方法被用于評(píng)估個(gè)體comt基因第二號(hào)內(nèi)含子snprs737865多態(tài)性與氯氮平代謝的關(guān)系。用于本發(fā)明的測(cè)試樣品沒(méi)有特別限制，對(duì)于檢測(cè)snp而言，可以是從分離的血液、組織等樣品中抽提出的dna或mrna；對(duì)于comt基因第二號(hào)內(nèi)含子活性而言，可以任何含有comt基因第二號(hào)內(nèi)含子的樣品，如分離的血液等。
所述的“分離出的或純化的”是指在基因組dna水平上，利用引物對(duì)來(lái)擴(kuò)增每個(gè)多態(tài)性位點(diǎn)的基因序列和利用純化試劑盒對(duì)擴(kuò)增產(chǎn)物進(jìn)行提純。
以下是對(duì)本發(fā)明方案的具體實(shí)施過(guò)程：
本實(shí)施例采用的樣品(具體為血液樣品)來(lái)自上海市精神衛(wèi)生中心的995個(gè)精神分裂癥患者，年齡18～60歲；全體參與者均由家屬在正式的知情同意書(shū)上同意簽字。本實(shí)施例涉及的comt基因第二號(hào)內(nèi)含子的序列見(jiàn)seqidno：1，其所編碼的氨基酸序列列于seqidno：2；comt基因第二號(hào)內(nèi)含子的基因組序列可以從genbank中獲得。
引物設(shè)計(jì)信息：
采用primer5.0軟件，以genbank數(shù)據(jù)庫(kù)comt基因(id：1312)第二號(hào)內(nèi)含子以及內(nèi)含子與內(nèi)含子接合部位序列為模板設(shè)計(jì)一對(duì)引物，由invitrogen公司合成。
引物信息，擴(kuò)增comt基因第二號(hào)內(nèi)含子snp位點(diǎn)的dna序列引物信息：
野生型引物對(duì)為：
正向引物：5′-acaataaaaagcaacaggacacaaaaaacc-3′(seqidno：3)
反向引物：5′-tgctgtgatgaggggctccagatag-3′；(seqidno：4)
所述突變型引物對(duì)為：
正向引物：5′-aaaaagcaacaggacacaaaaagcc-3′(seqidno：5)
反向引物：5′-tgctgtgatgaggggctccagatag-3′(seqidno：4)
pcr擴(kuò)增條件：(體系各項(xiàng)試劑由abi公司提供，也可以從公開(kāi)的渠道獲得)
反應(yīng)體系總體積為20μl，其中：
100μmol·l-1dntps；
50mmol·l-1kcl；
10mmol·l-1tris-hcl，ph為8.3；
1.5mmol·l-1mgcl2；
0.5utaqdna聚合酶；
20-50ng模板dna；
0.5μl10pmseqidno.3；
0.5μl10pmseqidno.4；
雙蒸水補(bǔ)足至20ul；
pcr反應(yīng)條件：95℃2mins；30×(94℃30s；58℃30s，72℃30s)72℃5mins。
上述pcr反應(yīng)完成后，擴(kuò)增產(chǎn)物經(jīng)非變性聚丙烯酰胺凝膠電泳后銀染顯帶，可以發(fā)現(xiàn)comt基因snprs737865野生型(基因型為a)擴(kuò)增產(chǎn)物比突變型(基因型為g)多了5bp，因此基因型為純合個(gè)體擴(kuò)增產(chǎn)物為單一條帶，雜合個(gè)體為雙條帶。比較發(fā)現(xiàn)comt基因snprs737865有多態(tài)性(即第858位a→g)。關(guān)聯(lián)分析發(fā)現(xiàn)comt基因snprs737865的g基因型與a基因型患者用利培酮治療前后有顯著的的個(gè)體差異，p值為0.007(見(jiàn)表1)。
本發(fā)明為研究我國(guó)人群中comt基因多態(tài)性與臨床用藥安全的關(guān)系奠定了基礎(chǔ)，為臨床用藥個(gè)體化提供理論基礎(chǔ)，同時(shí)也為基于藥物基因組學(xué)理念的新藥研發(fā)提供指導(dǎo)依據(jù)。
表1
以上對(duì)本發(fā)明的具體實(shí)施例進(jìn)行了描述。需要理解的是，本發(fā)明并不局限于上述特定實(shí)施方式，本領(lǐng)域技術(shù)人員可以在權(quán)利要求的范圍內(nèi)做出各種變形或修改，這并不影響本發(fā)明的實(shí)質(zhì)內(nèi)容。
sequencelisting
<110>上海交通大學(xué)
<120>基于rs737865的檢測(cè)利培酮用藥效果的試劑盒
<130>dag28395
<160>5
<170>patentinversion3.5
<210>1
<211>28236
<212>dna
<213>homosapiens
<400>1
cggcctgcgtccgccaccggaagcgccctcctaatccccgcagcgccaccgccattgccg60
ccatcgtcgtggggcttctggggcagctagggctgcccgccgcgctgcctgcgccggacc120
ggggcgggtccagtcccgggcgggccgtcgcgggagaggtgagagcgctggctagaccgg180
ggccgaatgcggccggattcggggcgggggccttcagacctagggtggaacactgggata240
gggtgtggggaattcggaccgctgtgaagtgatctgacgttgggtgggagtctccggact300
tggggtggggaatctggatgggaactggggaattcggaccttgggtggggatctttggac360
cggggtggggtccccagactccttgtggaaagctgaggttgaagatctgtgctgtgatga420
ggggctccagatagcggtgagagatcccgcttcaagactgggttgggggtccagaccagg480
aggataagcctctccctggagtagggcttgacctggtgagggtgtggtagagtcacaggg540
gagggtcccacttggtccttgggctgggatcagactctcagagctggagagttgcaggag600
gcctgggggagggtctgtggagccccagactcagagggccttggtgacttctccaacagg660
ctccctgttggaggccttggggtaccccagggcctttccctgcaagagagaggctgctcc720
accaggaaggggcccaggactccccagggtcggggggatgtgtggtgtgcaggaccacgt780
gggaatgttagagaaaggggaagtcactccgggcctgccctgctaacagacctgcttttt840
ggatttttccagccagggatttttgtgtcctgttgctttttattgtttaaagtgaccctc900
caagcctgagggaccgtaggagctgccctgcagagcccagtgagacactagttaatgcag960
aaaaaacagattttacttggtaactactgacaacatgagaaaaagctggctccatgctga1020
tttgtctagatgtgcctggagtttaaagggagagtgagggagtgggaaggggtggggact1080
cagtagagttagggaaacagaaaatcaccaaaactgggaagggagggttggcccctgtga1140
aaccaactgatctgggtttgccactggggcttaccgaagtcaggcccccaacctcccaca1200
gagactgggagacaggggccccatcctcaggtgttggctggaacacacaggaaattcttg1260
tggcagccttgaacttctcaggcaggcactttagagggggctaggatcgttctgggaatg1320
tggccttgagctgttaggagctatgttagtgttcgttccagcctagataggccagggtga1380
ggcccagtggagaaggggactctaaggagcctggctggagtgtggggagggagggtcttt1440
gtcagcactccccctgtgcaagaagagcagagacctgcttctttcctctcaacagtcccc1500
gtccattttctgttctgtgttgtgttcataaactaccagcgggaccatctgttgagactt1560
ggtagcaaaggatttttgctggatgatgcaagcagtcaagcatttaatgaggaaacaaaa1620
ataacaggccaggcacagtggctcatgtctgtaatcccagcacttttggaggccgaggag1680
gatcacttgaggccaggagtttgaaacaagcctgggcaacataaagaccccatctctaca1740
aaaaatttaaaaattagcccggcgtggtggcatgggcctatagttccagctacttgggag1800
gctgaggtgaaaagatcgtttgagcctgggaggcggaggctgccatgagccatgatcttg1860
cctctatactccagcctgagcaacagagagcccctatctttaaaaaaaaaaaataataat1920
aaaataaaaaaataaaaagaaagaaagaaataaaaacaaagatgaatggagctgttgata1980
aacccagtttttgagcctacagggcagcagttgaggtttctggccgttgggccctaacaa2040
ggccagcaggggcactcagggacttcctggtgtgtggtgtgaagtctgtgatgatggcat2100
aggcattggtatcccagttgtggttactttctggagagagcatgtggcatgcaggagctg2160
gagggggggtcttcctgggcggcctgtgtagcagcagctccaggcacaaaggctgcccct2220
ggagaatctgttgtggttctgagttctttcaagtttatatcaagttttccggcttcagtc2280
tgcagggctgtttttttcagatcctgggggcaaagggcagctacaagaccacctgagtcc2340
caatagggatttgagttcttggtgacaccaagtcagggaggagacaaattagaaatgtca2400
gtctgaagagagtggtaggtagccagatactgaaggaaagtagatgggaggatttggtaa2460
gggctgacgtgtaggaaggcagtgacagtccaagtgacaggcagatcataaaacctcaag2520
gataggccgggcccggtggctcatgcctgtaatcccaagcacttcgggaggccgaggtgg2580
gcaaatcacccgagtccagaagttcgagaccagcctggctaacatggcaaaaccctgtct2640
ctactaaaaatacaaaacattagccgggcgtggtggtgcactcaggaggctgaggcggga2700
taatcgcttgaactgggaggtggggggctgcagtaagccgagatcgcaccactgcactcc2760
agcctgggcaacaagagcgaaactccatctcaaaaaagaattaggccgggagcggtggct2820
cacgcctgtaatcccagcactttgggaggccgaggcgggtggatcacgaggtcaggagat2880
cgagaccatcctggctaacacggtgaaaccccatctctactaaaaatacaaaattagcca2940
ggcgtggtggcgggcacctgtagtcccagctacttgggaggctgaggcaggagaatggtg3000
tgaacccgggaggtggagcttgcaatgagccgagatcacaccactgcactccagcctggg3060
cgacacagcaagactgcgtctcaaaaacaaaacaaaacaaaacaaaacacctacaaaaga3120
cagtgaactagactaggatctgataactcccaagggggtgttatatttctccactgaagc3180
atagcatttctctttacaaacccttcccttttgattgaagataatcaaagtgagattatt3240
cttatttaccaaataattctggtctcattaaatttggcctgattatttacataaatgcag3300
cacgaacagtaattgaccacacaggcctttttaagtttgctgaaacttttaatagggaat3360
cttagattgagctttttaaagcctttcgaggttaggaagccaagccaagaacttccaacc3420
agacttcacctgcagtgtctatagatttgggtgaattcctctcttctcgaggtccctgaa3480
atatcccaaggttcctgggccttagaaccttgggatatttggaagtgactttccttactc3540
acatgtaagaccaagaaccccgtaagccacataccaggccaaatttttcaagagctcttt3600
gtaagcaatggctccacaaagtcaacctgaaagccatctggtcatagctgattctatgtg3660
catcatcctcaaacaggacattccagtccaaaccttggtaatagaaccagtgtttccaat3720
tgtgtcctgttacaaggagaacagattcttattgaagttattcaataactatattgccat3780
gaaaatagaatactcaataatagtttctgatttttggaggggtcaggccggaagaaaaag3840
ataattgcttcaattttgtttagagaagtatcatttactataatattacgagtcattgat3900
aggttaagagaaaagggaaaggtttctttctttttttcttttttgaggcagagtctcact3960
ctgttccccaggctggagtgcagtggcgtgatctcagctcactgcaagctccgcctcctg4020
ggtttacaccattctcctgcctcagcctcccgagtagctgggactacaggtgcctgccac4080
cacgcccggctaattttttgtgtttttagtagagatggggtttcactgtgttagcaagga4140
tggtctcgctctccagacctcgtgatccacccgcctcggcctcccaaagtactgggatta4200
caggcgtgagccaccgtgcccagccggggaaaggtttctttttttaatattcagaaaata4260
aaacataaagagccaacaatattctgaacaaaagtcataaaaagactgtaatcatccttc4320
agcagtttactcagttgcatgcaggtactgaattcttgttctgcttgatcttgggctagc4380
aggtacatgaacccatcttttctcaaccagtggagatcctggctcagtgcagtcatgtga4440
tctcaaagttaagaaatgctgtcacgcccaggtgcagtggctcaggcctgtaatcccaac4500
actttgggaggccaacgtggctatatcacttgaagtcaggagttcgagaccagcttggcc4560
aacatggtgaaaccctgtctctaccaaaaaatacaaaaattagccagttgtggtagcaga4620
cgcctgtaatcccaactactcgggaggctgaagtgggagaatcgcttgaacccgggacat4680
ggaggttgcagtgagccaagatcacgccactgcactccagcctgggcaacacagtgagac4740
tccatctcaaaaaaagaaataaagaaatgctgtcagaagcctaccccatggtacctgtca4800
tgggccttttcattggtccctttttgttgaagatgaagcactttgccctgtagctgattg4860
caagagatttcaggaaagcatcagagtaaaactctaatgacaaaagacttaaaatagtgt4920
agttaaatatctaatgagagatcattacaaaatcaaacgatttacaaggaatgttggttg4980
tttatgacacatagcattttaaataataaccagaatgatgattgctgttatatgaggaca5040
tgtcattggcagaagggtattcaccagtttgtgggaactttatacaatatctggaatcct5100
tatattaacatttaccaatatgcatgtaactcaaggaagtttaaatagcattaaaaaaaa5160
tttttttttagtttttttttttttttttttttttgagacagagtcccactgtgttgctca5220
ggctggagtgcagtggcacgatctcggctcactgcaacctccgcctcctgggctcaagcg5280
attctcctgcctcagccccccaagtagctgggattacaggtgtgcaccatcacacccagc5340
taatttttgtatttttagtagagatggggtttcgccacactggccaggctggtcttgaac5400
tcctgaccccaggtgacctgcccaccttggccttccaaagtgttgggattacaggcgtga5460
gtcaccgcgtccggccctaaataacattttttatttgacagcaccttgcatgaaattctg5520
tagggtccagccctacagggtctgtgggtttttctccccgtgtgcagagatgagagatcg5580
tagaaataaagacacaagacaaagagatagaagaaaagacagctgggcccggggaccact5640
accaccaagatgcggagaccagtagtggccccgaatgccaggctgcgctgttatttattg5700
gatacaagacaagggggcagggtaaggagtgtgagctatctccagtgacaggtaaggtca5760
catgggtcacgtgtccactggacgggggcccttccctgtttggcagccgaggcggacagc5820
gagaggagacagcttatgtcattatttctgtatttcagagacctttagtactttcactaa5880
ttttgttactgctagttagaaggcatagccaggtacagggtggaacatgaaagcggacca5940
ggagcgtgactgcttaagcacagcatcacagggagacggttaggcctccggataactgtg6000
ggcaaacctgacactccacaagaggtggttgagcagagtcttctctaactcccacaggga6060
aagggagacttcctttcccggtctgctaagtaacgggtgctttttctaggcactgacgca6120
actgctagaccaaggtccgctaagtaacaggcgtcttcccaggcgctgacgttaccacta6180
gaccaaggagccctctagtggccctgtccgggcataacagaaggctcacacgcttgtctt6240
ctggtcacttctcaccgtgtcccttcagctcttatcactgtatggcctggtttctcctag6300
gttataattgtagagcgaagattattataatattggaataaagagtaattgctacaaact6360
aatgattaacaatattcatatataatcatatctatgatctatatctaatgtaactattct6420
tattttatatattttctttattatactggaacagctcgtgccctcggtctcttgcctcgg6480
cacctgggcgacttgctgcccacaaaattcaccattgaaataaacctaattagtttaaca6540
tctctcttataaggtcacaggacagatcctttgaggtcttccaggggccctccaggaaat6600
ctcaagtcagtctgagatcaagacaatgtcacaggccacatgcagtggttcacgcctata6660
atcccaacactttgggagtccgaggagggaggactccttgagcccaggagtttgagacca6720
gcctgagaaacatgctgaggccctgtctctacaaaacaaaaagttgaaaaattagctggg6780
catggtggtacatgtctgtagtcccatgtactctgaagactgaggcgggaggatcacttg6840
agcccaggagttcaaggttgcagtgagctgtaattgcaccactgcactccagcctcagta6900
acagagtgagactctgtcactaaaaaaaatttaggctgggtgcagcggctcacgcctata6960
atcccagcattttgggaggtcaaggtggacagatcacctgagatcaggagttcaagacca7020
ggctggccaacatggcaaaaccccacctgtactaaaaatacaaaaattagctgggcgtgg7080
tgccatgtgcctgtggtcccagctacttgggaggctgagacagcagaattgcttaagcct7140
gggaggcagaggttacagtgagcccagatcacaccatggcactccagcctgggtaacaga7200
gcgagactctgtctcaaaaaaaaaaaaaaaaaaaaaaaaatttaaagaaactttatacat7260
aaatccattcaatgattatgaggtcaattctagccagctttgacctcataacataaaatt7320
tcttttctgcagactttcttgaggccaggagttcaagaccagcctgggaaacatagggat7380
aactttctgtatccattcacagttgcttttgttttgagacagaatctcagtctgtcaccc7440
aggatggagtgcagtggtgagatctcagcttactgcagccttgacctcctgggctcaagc7500
gatcctcctgcctcaccctcccaagtagctgggactacaggcatgtaccaccatgccagg7560
ctaataatttttgagtttttttatagaaacagggtttcatcatgtttcccaggcttgtct7620
cgaattcctgggccattcaggctttgtcccatatttttcctctttttgtaattttacttc7680
aggacaaaattacatccctttctttaacaaaaacactctacattctttgcatacttttca7740
tataaaaacacatcctatcttcctcccatactttgtataggaaattgtttccctcattct7800
tattgtttctaggagttttgttttcatatattgattataatttttaaccattagtaaccg7860
gtattttttttttaaagagacagcgtcttgctcttttgcccaggctggagtgcagtggca7920
caatcaggactcactgcacccttgaactcccgggctcaagtaattctccaacctcagccc7980
cctaaagcactgagattacaggcatgagtcaccacgcccggccataacttgtattttaca8040
agtaaaactgggaagaagacaattggaaattgtctgtcatttaccagtttgttggcaaat8100
tttatgaatataccatcttataatctctagaggtatatcctttttatagtacaatgtttt8160
taagtgccaagtacatgtttattaacagaccagaatacctttagccagtctgtaccatat8220
aaaaataagaagccaaaaagtacaaaaagttacgcttaataatgaatgtttcagcacttt8280
cttctcttcaggtattatttagatattcaatgaatgtctattatttaacttcacttagta8340
tgactctgaggttgaaagttactgaaaacatcttgtaagcttttttaggccaatatatta8400
tcaaacgcaattaacaaaacacaactcactagtttatataagagctagttctcacttttt8460
tttttactctattttatatattttttcttttcttttcttttctttttttttttttttttt8520
ttttctgtagagacaaggtcttgctgtgttgcccaggctggtcttgaactcctagcctca8580
agtgatccacttaccttggcctcccaaagtgctgggattacaggtgtgagccaccaccca8640
gccctattttatatttttatctgaactgtgtttctggcaaattggacaagttaagggtca8700
cttgctccatgtgaggggaaggctttctacctgtatttgtggataagacctttacgactt8760
gttttgccctgatggatgactacatgtttccagtgagctcagctggttgcttttgtgggt8820
ccctgaggcactgcgagcccctgatggggcaggaagcctaaagttccagggaccgtgggg8880
agttgaggggctggagggggaagagagaggcctgcagcggggatccacaggtggaggagg8940
ccagacttgaagggggtgtctccttcaagagaaagtagggaagtatggaggtgataggaa9000
gggagaggcctgagaggagctagtttggggagatctcaagtatcccaaaggagccactga9060
tgttccaaattatccttggtaaagtcacatcaacaaggaaggaagcagacagaggcgcac9120
ttgttccttgcagttttgttttgatgtgctgattaaagtagcgcagagggcatgatcagc9180
agggctccagtaagacgctcccacgggaggagcaagaacacagaacagagggggcaagac9240
agctccaccaggagtcaggagtgaatcccctctgggaacgaggcactaggaagaagaact9300
tccagcccaggaggtaggtagcgttcagaagaagcctggggcgggcgcggtggcgcacgc9360
ctgtaatcccagcactttgggaggccgaggcgggcgaatcacgaggtcaggagatcaaga9420
ccatcctggctaacacggtgaaaccccatctctactaaaaatacaaaaaattagccgggc9480
gttgtggtgggcacctatagtcccagctacttgggaggctgaggcaggagaatggcatga9540
acccgggggacggagcttgcaatgagccgagattatgccactgcactccagcctgggcga9600
cagagcaagactctgtcccaaaaaaaaaaaaaaaaaaagcctgggactcttagcgcctca9660
gagccttactcagaatcataaaatcattagaagcaatgtctgtcctcactgctgcatccg9720
atattcttccggaccactggatcacaaatcaaaccatgtgactcccgcatgagcaggcag9780
agtggagataacacggatcgctgtgtacactgtgtgctccggttgttgcatccgagggtt9840
gatcggatggtggttcccatccagatccaagtcctggcccctgatcacagagaaacacag9900
ctggacattaaagtggtgaggacggaaattataatatttggtgaccagtgacaggagggg9960
agagagccgagctccgctccgagttgtgcaaagaagtggggagtggggaggaagaacagt10020
ggggactcagtggagtgaggaaagtggaaaattaccaaaagcaggaagggagggttagcc10080
ttgggaaaccaatctgggtttgccacgggggcttactgaagtcaggcccccagtcccaca10140
gaggctgggagacaggggccccatcttcaggtgttggccagaacacaggaaattctcttg10200
gtagccatgactttctcaggcaggcgctttgaagggggctgggatcattttaggaatgtg10260
gccttgagctgttagaagccatgtgcagtatggcttacacctgtaatcccagcactttgg10320
gaggctgaggctggagaatcacttgaggccaggagtttaagaccagcctgggcaacatag10380
caagacgctgtctctacaaaaataatttttaaaaaattagccaggcactgcggcatgccc10440
ctgtgtagtcccagttactcaggaggctgaagcaggaggactgctggagcccaagagttc10500
aaggttgcagcaagctgattgcaccactgcactccagcctgggcggcagaggtgatcacg10560
ccactgcactcccgcctgggcgacagagcaagaccctgtctttaaaaacaaaaaggccag10620
gcaggtgcggtggctcatgcctgtaatcccagcactttgggaggccaaggcgggcggatc10680
atgaggtcaggagatcgagaccatcctggctaacatggtgaaaccccgtctctaccaaaa10740
acacaaaaaagttagccgggcgtggtggcgggcgcctgtagtcccagctactgggaggct10800
gaggcaggagaatgccatgaacccgggggatggagcttgcagtgagccgagattgcgcca10860
ctgcactctagcctgggagacagagcaagactccgtctcaaaacaaaacaaaacaacaac10920
aaaaaaaaaacagccgagcgcagtggctcacgcctgtaatcccagcactttgggaggccg10980
aggtgggcagatcacaaggtcaggagttcaagaccagcctgaccaacatggtgaaacctt11040
gtctctactaaaaatacaaaaattagctaggcgtggtggtgtgagcctataatcccagct11100
actcaggaggctgaggcaggagaatcgcttgaacccaggaggcggaggttgcagtgagcc11160
gagatcgcgccactgcactccagtctgtgtgacagagtgacagcccgtctcaaaaaataa11220
ataaataaataaataaataaataaataaataaataaataaataaataaaatgaagcagca11280
gcagctttgttagtgtttgttcaaggcctgataggccaaggtgaggctcagccgagaaac11340
gtgctcagaggagcctggccggagtgtgatgggggagtctttgtccaggctgctgccacg11400
gtgggcctcaggactcatggcctgggcgctgggggtggggctgccactcagccttgagga11460
gagcaatgaggaggtcagattccactcacatccagtgcggtctggatgcaggcctggccc11520
agagcctggtccagatgctggggctttgtttttttgttgttgtttttttgagacagagtc11580
tcgctctgttgcccaggctggagtgcagtggcactatctccactcactgcaacaacctcc11640
acctccacggttcaagcgattctcctgcctcagcctcccgagtagctgggactacaggca11700
cccgccaccatgcccggctcattttttgcatttttagtggaaacagagtttcaccatgtt11760
ggccaggatggtctccaactccagaccttgtgatctgcctgcctcagcctcccaaagtgc11820
tgggattacaggcgtgagccactgcacccagccggctttgtttacagtactcagcagctg11880
ccaaaatagtccccattcccaggcagggcaggctcccgggctcagtccagcggggcagat11940
ggccagcctgcacgtcaccccactaggcatgtccaggccctttctgcccctccctaaagg12000
cacccctgtcctttctgtcccctccacactgagccaagccagcctccactcttgccccag12060
gcctgccctctgctgggagccgggacactgagctcctgccacatgagcaggttgacctgc12120
agggggcctccaagcaggtgccaaggcagaatcggatgtgaccaggagaacacccttgaa12180
ggataaagcgaatgtgcgaccaccatgcaggtctggcccatggaagggaggggagggggc12240
cccgacggggccacagtaaaggagtggaggggccccacaggcagggatggcccagctggc12300
cccctggggctgtgaatctgcaggtgcagtgcagcctcttgcctctctcagcccagccct12360
tgcatctgcctcttggtgtgtctgtgaagacaaagtcaccgtggtccctgcagcacctgg12420
cttgaagcaggtgtgcagtccgtgtgaaagccttccctttagctattaggtattgagtca12480
aaaaaaaaaaaagccttcccctgagcctgggggcctggccccactgaggataccaggcgg12540
gggaggctacaggaggctgccctctgcctggccatctgaccttccattctgaccccttcc12600
cttccacaccagcccctttccctatgtagggtgcagtctttgttgcaactgctcacctct12660
gccatggtcggatgagagcagccacggacaatgtctgtgcaattgtgcgttttttgtttt12720
gttttgttttgagatggagtcccactcttgttgcccaggctggagtgtaatggcaccatc12780
tcggctcactgcaacctccacctcccgagttctagcgattctcctgcctcagcctcccaa12840
gtagctgggattacaggcatgcatcaccatgtctggctaattgtgtatttttaatagaga12900
ctggggtttcaccatgttggtcaggctgttcttgaactcctgacctcaggtgatccaccc12960
gcctcagcctcccaaaatgctgggattacagacgtgagccaccgtgcctggttgattgtg13020
cgttctgataaagcttcatttacagaaatagcaggtaggcccctcgcagggtgcccagag13080
caggtgcactgtctcacctcttctcaggtgtcaccggggtgagaggctagtcctctgcct13140
cctccttcctctctgcaggttggagaaatgacagcttcttgtactgggggttatgagaca13200
gtggcaggagggggacactcccagagtgctgccagaaagaggcgaggccccatctggatt13260
tgatggcatttttattgtggtgttgttttagagacagtgtctgtcgcccaggatggacga13320
catggtgcaatcatggctcactccagcctccaactcctgggcccaagcaatcctcccact13380
tcagcctcctgagtagctgcagctataggcacaccaccatgcctggctcaatgggtattt13440
tgtggccattaaacttctggtcattcctttagattcccagaggctggatggaggtgagtg13500
gccagcccctgggtccagagatgagcgcggggcctggctgcagcctgtggggtctcccag13560
gctggcctccaggtgtcctctcagcctgtccacaggcgctgcccaagcgtgtggcctacc13620
tggtgctgcctggcctgtctctacgcacctgtactggctctccctggattactggatcgc13680
agtcgaatcccctgccagaatcctggtccccctttatcacaccggatcagccccaaaggg13740
cagggcttcttgcagcagttccagcctttgctgggggtttccagccctgttgagcaagcc13800
ccaggtagggccctctgcagcccccgtgggggacctgtgtgccgagcagagctgccccgt13860
gtgtaaacgcttagaactggcctccttcctgccatctgtgtcctgtttgtcatcacgcct13920
gtggctctatgcttgtctccccgactgggccaccagccctgtgagggcaggatgaggcac13980
gtccaagccacagtggctctcagtgtgcagggtgctgttagcattggttctgtgttctgg14040
aatagtgagaacagtgtgccccaaatggccagtggcctttccgagggtcactgcagccgc14100
atgttggccctctgtggagaacggaggatgcacagccatttcagagcacaacctgaggtc14160
tcctgagcttgctgtgcagcccagtctttctctccgtggcccctggtagctgtctttctc14220
ccaggaggaggagaaagacaccttcctgagcaggaggatgtgggtgaatagagaccaaag14280
tgtcaatcactcttctgacagaggtgatagcagaatgaaaaactgatggaccccagatct14340
ttgcaaagagaacaaagtcagtttctagttatctttctttcttttttttctttttttctt14400
tttttttttttttttttttttttgagatggagtctcgctctgctgcccaggctggagtgc14460
agtggtgtgatctcagctcactgcaaccgcctcccgggttcaagtgattctcctgcctca14520
gcctcctgagtaaccaggactacaggcacacaccaccacgcccagctaatttttgttatt14580
tttagcagagacagggtttcaccatgttggccaggatggtcttgagctctcttttttttt14640
tttttttttttttttgagacggagtctcgctctgtcacccaggctggagtgcaatggagt14700
gatcttagctccctgcaacctctgcctcccaggttcaggctattctcctgcctcagcctc14760
ctgagtagctgggactaacaggtgcttgccaccaagcctggctaatttttgtatttttag14820
tagagacagggtttcaccatattggccaggctgatctcgaaatcctgaccttgtggtctg14880
cccgccttggcctcccaaagtgctgggattacaggcgtaagccaccacaccaggcccatt14940
ttctagttatcttttacggattttttaaaatgagcctagaaaccaggtcatataattagt15000
ttattttttaatttcttatagagacagggtcttactatgttgcccagagtggtcctgaac15060
gcctgggctcaagtgatcctcctgcctcagcctcccaaagtattgggattacaggtgtgg15120
ccactgttccagccatagttcatattttaaaggatttttaagagtctcatttcactgatg15180
ttacttgcagtatatttatggaaaacattcctgtcttttttttttttttgagacggagtc15240
tcactctgttacccaggctggagtgcagtggcgtgatctcggctcactgcaagctccgcc15300
tctggggttcacgccattctcctgcctcagcctcccgagtagctgggactacaggtgcct15360
gccaccacacccggccaattttttgtatttttagtagagacagggtttcactgtgttagc15420
caggatggtctcgatctcctgacctcgtgatctgcccgccccggcctcccaaagtgctgg15480
gattacaggcatgagccaccgcgcccggccagcattcctgtcttttaaagaaagatgaga15540
ccagatgcagtggtgtgcacctgtggtcccagctactcaggaggatgccttgagcctggg15600
agttcaaggctgcagctgcagtgagccttgatcacgccactgcactccagcctgggcaat15660
tgaacaggaccctgtctctaaagaaataaaataaaggcagatgaatgtcatctagggtgt15720
gtccaatgtgagagagaatactgaccaggtgacagaggtgatggactctctagctctgca15780
gcagactgctgggcagagaagcttgggcaggcctggcactgaggcccagaagcttcatgc15840
tctttagagccagaggtgtcaagcccagctgcctgtgtcctggccgcactgtgaggcact15900
gaggatgccctcacacgtgcatctgcatgtggcgtgcatgtggcaagcatgtgcaggtgc15960
tgtgtgggcacgatcatgcctgccctctactgtgctggagaggtaagaccatcagccgtt16020
tcacagaggagggaatgtggacctcacgggggtctcatgagcttgcgagccgatggccag16080
gcagccggtgcagaaggttcatccattttgatgaaccgtgaattcatctgtactttattc16140
attttaataactgaatcatacctcattgtatggctctaccatgttttattatccattcag16200
gtgctaacggatggaagggttgtttctacctgttggccattgtggatgatgttgctatgc16260
atattggggtacgagtatctggtttagtccctgttttcaattcttggtatacacctagga16320
gtagaattgctgggtcatatggtagttctattttaacttttttttatttttaatttttgt16380
gggtttttaagaattttttaatatttgtgggtgcatagcatgtttaattttttttttttt16440
tttgagacggggtcttgctctgttgcccaggctggagtgcagtggcaggatcacagccct16500
gtgcagcctcaacctcctgggctcaggtgatcctcccgcctcagcctcccaagtatctgg16560
gactacacgtgcggaccaccacgcctggctaatttttaagttttttgtagagatggggtc16620
ttgccatgtttcccagactggtatgggactcctgggctcaagcaatcctcccatcccaac16680
ctcccaaagtgctggaattacagtcatgagccaccaggtctgaccacatgtttaactttt16740
tgaagaactaccaaactgttttccacggcattcacaccagcaatgcaagagggttcccat16800
ttcatcacatccttgctaacacttgttattttccttaaaaaaaaaaaaaaaaaaaagcca16860
gctgggcacggtggctcatgcatgtaatcccagcactttggaaggccaagggggtggagc16920
acttgagctcaggagactagcctgggcaaaatatcgagaccccacctccactccatctct16980
accaaaaaaaaaaaaaaaaaaaagctgggtgtgcggtggtgcacatctgtcgtcccagct17040
acttgggggggctgaggcaggaggatcccttgagcccaggaggtcgagactgcagtgagc17100
tgcgaccgcgccactgcactccaggctgagcgacagagtgagaccctgtttcaaaaaaac17160
aaaaaaaaagcacatttcaaacatacacaaaagcagagagaattcccctcggtcctccgg17220
cctgcaccccaccgccccccaccacaggggcattcaaaactgtctttttgcctctctgta17280
aggtaaggggggactcttgacgctccccagaccacacagccaaagtagggcgatcggatc17340
atagggggtcccgagtcggacactgagctggcgtgcccactctcgagtcctgggacccca17400
aactaaaggagctgttccttttccccatggagggctgacggggcaaaatcccagggctgg17460
gaacatccgagcagctttgagtagcacaggtgggtttctacgtggctttctttaaaggga17520
ggcctgattctcggcttttcaggacccgccctccattttctcacttgcctgctctttcgt17580
cctgcgaaactgggcagtggccggtgacagcgcagggtcagccctcggatggccaggtgg17640
gcgtgatcccaccctcctccgtggctgggtgggcgtggtcccaaagtcaattggatgtgg17700
tcccgcgtccctcccggcccagtgggcggggcctccccttctggggcctcccatcccaca17760
ccagcgagcagagaggagggtgggcggggaggccctacggggctggggcggggcggagcg17820
gaggtggttagctgtcgggaggtggggtgggcaggggccagggctctgttggtggcccgg17880
ggcagccagtccctctgctcagggcttcagttcacactcacaagggaatcccagcgctct17940
tctcctctggcggttgctggagggccttgtgaggtggccgtgggcactgtagcccccagg18000
cactgcattgtggcctgggcgtgttggcctttgagcctttggccttgagcacttccccat18060
cccccatggctcttggccgttggggcccagttggccgcagagcctttggccttgagcact18120
tccccagcccccatggctcttggccattggaccccagttggccacagagccgcgggcagg18180
tgggacaggcgggcactgggtgcctccttgcaccagccaggcccagcctgcagagtggag18240
tgggccggtccctcctgccaggcaaaggaagggggcttcgggcccctccctgctgtggcc18300
tcctcccctctgctgcctcccactaggccctgggagacactgcctgcagatttatgaaca18360
gacagacctagagattagagattcaaaagccaaattcttacacaattcaagtattaacgc18420
agatttggatttataatttgggactccaaggtctaccaagtaatttgctgtttcagaata18480
gcccttttaatttggggtatatatttctaacactgttttttaaatatggagagtaaacaa18540
aatggcccattatctgaccacacaaatactagtagtcattatagataaaccatagcagat18600
aaataatagtaaacaaagcaacaggctgtgtcataggaaatccccaccatgaagaaagga18660
gcaaggtggaaagttctggctgcttcaggtctgcatggtccctctccaccatggttcccc18720
ctgtcatcttcctgccagaataaggacactgtccttagggaagcaccatctcttgttttt18780
tccccacgagccctgtgggtcatggcacgtcctgccccgctgggaaacacagtgggccac18840
gggtttccctgcaggcctgggacccttcccaagggtagcagcagaaggcagcacgattcc18900
cactcctgcagctgtggacaggggcacccccactgtcactgagccctgcaccgggttcca18960
tcacctgctcggggctctggcctttggccttttcctgtgagctgcatgttggccactgtg19020
acctatctgtctcatcatctttttcttactggtttgtgtatgttcttggtaaactagccc19080
ttggtcttacacatcatttccaaggtactaaggactcttcaggggaaatacaacttgagc19140
agagtggttccctcctcttgtggttcacaaggtgcaggtgcacacacacatagcccacag19200
ggcagtgtggacagggaccaggagactgcccctggggtccctggctgggggacactagta19260
gggatgtcccttggcctctctgaggccttctgctgtctcttctgaggccggaaaggcgaa19320
gcactgccctcgccctgctagggaaggctcaggccaggctggccctatccggggaagggg19380
ctcaggtatctggaccttggtcatcgccaggttagggtttatgttgatgattatccaaag19440
gcaaaattgatttccacagaaataacatctgctttgctgccgagctcagaggagacccca19500
gacccctcccgcagccagagggctggagcctgctcagaggtgctttgaaggtgagttggc19560
caacggaagccggggcagtgccagggtgggtacagattccggcccggtgcatgggcacag19620
gtctgctgagcacatgtccactctgcctgtgtaaataggccacatggcctgaaatcccct19680
agaagcctggtgtccgcatgacctccccctagggcggagcctctgcttccctgttctctt19740
ctgctctgtcctctggtgccctgaggctggcctccaggggtgtcccctctgtggccctag19800
gcctgcctccttgcctgggtgtgcctttctaaaatggagcgtccagcagagagtgggatc19860
tcctatgccactgggagcccagggccccatcccagaaagacctctgagtgagcacagggg19920
ccctagaagaagtttccttgtgtccttcccgttttagggtctgtgacctgaacccctggg19980
cttctgcctcaggcctcctgtgctctgctctctgcctgctgggtcccctcaccaggcttc20040
tgtctggttcccaggctacctgcctggagggtcacaccaggaggatttcaaacaggtttc20100
aagtggggtcacttgccatcactgtgccccacgaggtacactgttgtgggcggcagggct20160
ggcctttctcatctgggacatgccacgttgctgttcccaaggggagtggtgagtgggtct20220
gtcctggtgtgcctggcctggggactgccagtgtccttacttggacactcaatgaaaagg20280
ccacatgaatccctggggcgtccagagcatgggtgaccagcacgggctcaccacatgagg20340
ccaaggggctgcaccatacagcctctcctttggccacccgtgactacccccaactccggg20400
ccatggggcttccctacccctgggtgtcctctaagccagctgggagacaacagcctgagt20460
ccgtgtctgcttctgtattttgtgtggttttagaggatccctgggctgcctggggaagca20520
cccagggccagggagtgtgaccctgcaggctccacacaggactgccagaggcacacacct20580
gctctgtctacccgagggcaccagagggcacgagaaggctggctccctggcgctgacacg20640
tcaggcaactgaggcacaaggctggcatttctgaaccttgcccctctgcaaacacaaggg20700
ggcgatggtggcactccaagcaaaggggcgtgtgggtgctgcaggaggagcacagagcac20760
tggcgcccctcccctcccgccctgcagatgccggaggccccgcctctgctgttggcagct20820
gtgttgctgggcctggtgctgctggtggtgctgctgctgcttctgaggcactggggctgg20880
ggcctgtgccttatcggctggaacgagttcatcctgcagcccatccacaacctgctcatg20940
ggtgacaccaaggagcagcgcatcctgaaccacgtgctgcagcatgcggagcccgggaac21000
gcacagagcgtgctggaggccattgacacctactgcgagcagaaggagtgggccatgaac21060
gtgggcgacaagaaaggtggggtccgggccagcaggtgctcagctctgggacagggaccc21120
aggaccaggcatcaaagcccttacaggagaagctgttatcaccccatttccagggggctg21180
ggaaccctgggatatgcccagatagggctggggggctcctctggagtcccagggtgccag21240
ggtccctgatgacccctgcaggccctgctgcctgctgccccaggacaacaggcccccaca21300
ctcacagggtctgacggtggtgcagttccccttgaactctgttctggccaccatgggacc21360
tgcctggggaccagtcagacaggttctcctgggcccgcctcccgcttgaacttcagcctg21420
gggcacaggatgtgttaccgggctcacggagtgactcagggaactagtgccgccccaggg21480
ccccaaggtgggcggttcggtgattcagagagggcagctctgtgttaggacacactgggg21540
ccagccaggaagggtggaaaagatagggaccagcgtgagcatagaggctaagggaccatg21600
ggagctccaagcgcgctcacagtggggaccaggtcctgggggctggggacaccagggagg21660
tgaaatacccctccagcgggtagggagggtgggcagaggagggccagcggccaggcattt21720
gggaggggctcctgctctttgggagaggtggggggccgtgcctggggatccaagttcccc21780
tctctccacctgtgctcacctctcctccgtccccaaccctgcacaggcaagatcgtggac21840
gccgtgattcaggagcaccagccctccgtgctgctggagctgggggcctactgtggctac21900
tcagctgtgcgcatggcccgcctgctgtcaccaggggcgaggctcatcaccatcgagatc21960
aaccccgactgtgccgccatcacccagcggatggtggatttcgctggcgtgaaggacaag22020
gtgtgcatgcctgacccgttgtcagacctggaaaaagggccggctgtgggcagggagggc22080
atgcgcactttgtcctccccaccaggtgttcacaccacgttcactgaaaacccactatca22140
ccaggcccctcagtgcttcccagcctggggctgaggaaagacccccccagcagctcagtg22200
agggtctcacagctctgggtaaactgccaaggtggcaccaggaggggcagggacagagtg22260
gggccttgtcatcccagaaccctaaagaaaactgatgaatgcttgtatgggtgtgtaaag22320
atggcctcctgtctgtgtgggcgtgggcactgacaggcgctgttgtataggtgtgtaggg22380
atggcctcctgtctgtgaggacgtgggcactgacaggcgctgttccaggtcacccttgtg22440
gttggagcgtcccaggacatcatcccccagctgaagaagaagtatgatgtggacacactg22500
gacatggtcttcctcgaccactggaaggaccggtacctgccggacacgcttctcttggag22560
gtgagccccaaccaggatggcatccgtgccagctgctgcccagagcccattcagtcagcc22620
tcagcctctccaaagagccaggcattccagtagagccctgtgtggacacagctcgctctg22680
gaggcaccacctgaggtctgggagtgtgggggactgaggaggccctgtggtgggtggaga22740
tgggtggggagctgggccaggggcctggctgggtggcctgttgggaactggggagccagc22800
tgcctgtgcaggtgcaaaatgggtggcagaagtggggtgcacaccccagaccagacacca22860
gggcagaaacggcacaggaccaaggagatggggtggggaagggccgctctgggcccagcc22920
tgctctcccccaagcaagccactgctcgtgcaaagaaagcatgtgtctcctgcagatctt22980
cctcctgaggccccatcttgtgcattcccccaacccagccccactggcgaggaccctgag23040
tgccccgagtgaggctagacagcgggtggggctgtcctcgcttccctggggggcgtgggg23100
cactggtggcccttcacagactgatgcttaaggagcctcacatcagtgacacactgcccc23160
atccctccctggtggtcagcgacactgagtggctctgtgatcctccactgggcttgggac23220
acccaccctcacggcctccccacctggtgctcgctcacctgcagctctcccagaaactgg23280
acactgctgttagcagccggactaggagcacgaggggcacagcccccatgcctggctagg23340
tagggccgctggaccctggacacggattggaaggaaccagcactagcagaagcctgaggt23400
gtgaaagggcagagaatgttccaggaacaatgggagtcagggcacacaggactttgggca23460
ggtgaggatgaggttagacctgtcttctggagctgggctcaggggctcatgcctgtaacc23520
cttgcactttgggaggctacggcaggaggagcgcttgaggccaggagagttcgagaccag23580
cctgggcaacatggcaaaagaaaaaatatatatatatattttagatggagtcttgctctg23640
tcaccaggctggagtgcagtggcacgatcacggctcactgcaacctccacctcttgggtt23700
caagcgattaccctgccttagcctcctgagtagctgggactacaggcgcgcgctgccaca23760
cccggctagttttttgtagtttagtagagatggggtttcaccacattggccaggatggtc23820
tcaatatcctgacttcgtgatccgcccgcctcggcctcccaaagtgctgggattacaggc23880
gtgagccactgcgtccggccgtattccagctttcaaaacaacaaaaaacaacaaaaactt23940
ttctggaaagatccctgtcagccttgtggagtgtggagagggctgtggggagggcatttc24000
agatgccctgaactcacgaggaggcactgaaccctggccgtggagagggaggacctgtag24060
tggccaagggggtgggcattgggagggtgggagggagacctacaggccaagacagggtag24120
ctggaggggggctcacccctgacaaaggagcatgatgctggcaattgggtattgatggca24180
gagagctggcactcagccagatgggcttcacttgggcaggaaagacacccacagctgggc24240
ctgcgttactgcggccgagtttcagcagctgtgatgtgggtgctaattacaggggcctgc24300
cttcatagaaaagtagcaaacattgcagtttagtaactaggaaactaatagttttctcag24360
tgctgcttgcgcaaagctggtaattatctcaaaagaagcaaagtcatgaagtgggaagtc24420
atgaattgggaatgggtgtccttgttaaacgcacatctgcacactcagggctggggccct24480
gtgcccccttgtggagggttaggggacaacctggctcttgtgagggtctagccatcccct24540
cagtgggttctgtgagcatcggaggcacgaggggtgaggggctcaggagcaggttgcaat24600
tcaaaatcaagggctgctttgaggaggcctctccaccgggctgctgtagtcaccaagtcc24660
agcccatgcccaaaggaagaggaatgagttcccccttaaaaaaaaaaaaaaaaaaagaaa24720
aagacagagtcttgctctgtgcccaggctggagtgcagtgatgacatcatagctcactgt24780
agcctcaaactcctgagctccagtgatcctcccacctcagcctcctgagtagctaggact24840
aaaggcatgcaccactacacctggctaatttaaaaattttttgtagaaatggggtctccc24900
tatgttgcccagactggtctcaaactcctggcctcaagcgatcctcttgcctcaacctcc24960
caaagtgctgggagtacaggtgtgtgcttggtctgaggctccaactttttgttgttgttt25020
ctcgagacagtctctcgctctgtcgcccaggctggagtgcagtggcgcgatcttggctcc25080
ctgcaacctctgtgaggctccaactcttgaagggaggagagtctaaggagggtgggccag25140
atgaaaaccacctcagcatagtgtcacctgctcctctgacactgtcgcttctccacggca25200
ttagattttcagtcctgctcagacgctgatgcatgtttagccagttctccaggtggtctg25260
agtagctggtaggaggcttgcagtgtcgggcgacaggcaggacaggtgctgccttctttc25320
ccctcttgaccagtttcgtaaaggagtgggcccctggcagcctccaaaggtgacccatgc25380
tcctttctgcccttccctccttctttcctgtttaactcgtgcaggtgcagtggtctggtg25440
tctttcagtccgctgacgtcttctgtatccctgatgaccagattgggctcctgagtcccc25500
tggcgcaacccgagaagtccaggagcccaggcccctcactcatgcattccctggcccaga25560
ctggaaggcagccgccctgctcaaggcctaggccattgtcctcctcccgggtggcgcttt25620
gttctacgtcttttcagctgacattgctaggacatttttttttttttctaaatgaaaaca25680
catcatgattcatggtggtacttcctgctcaactccggaccttggggctgtcccctgacc25740
tcactgaccttgcagccgtgtggtgtccatactgtcacatgaaagccccctgctttctct25800
gcagcatacaggctggaaacgaccgccacccaccaccaggacagctgcaagccctgtggg25860
actctccaggcccatcccagaggcatgtggggtcggataccagtgtttcaaggcacctgc25920
ctgcaaattgattttattatactttagatgtttgagattgcttgttaacttttgtttggt25980
gactaagtcaaatttccaagacaaggtgactggggtgtcccttgccctggtaggtccttc26040
ctccccataggcaaacacttcctctcaaggtttcttgtttatgtgatgtaagcaaaccct26100
tttctgatagcatagataggcaagcatcctatgaggtttctcccgatagtggaccccacg26160
ggccatgccctatggctgcgtgtccagtaacaactccttccttctgcacagaacccaggc26220
agaggaactctgtgtcctcccagggcccaggcactggtgaagatggggggtctgcaaatg26280
caggagcttggggatgtccagaactgaccccaaggggcaggcttgttgatgggaggtctg26340
ccccacctcagccctgcagggtcaccctggtcaggccaatattgtctccagggaccatac26400
cagcaacccctctccttgggtgcctctccctcataggcctgagttcctggcactgggtgt26460
tgagggccccattgtttccactcacccagctagcatttattgagcacctactgtgtgcca26520
catgctgttctaagggatggatactcctgagatggatacaggagttgatgagagaaaggt26580
ccctgtcctcacggggcccatgttctgaaggtggcacccaagtcttgtacagtcctttcc26640
tgcaggagtcacgctgggcagaaagtggaaacctggccccaggggctaggcacaggcgtg26700
gtgccgtggcctagtgaggagcacccatcctggtttggggcaggttctctgggcacctct26760
gaccctcacctcccccaccccccggtctgtttgcaggaatgtggcctgctgcggaagggg26820
acagtgctactggctgacaacgtgatctgcccaggtgcgccagacttcctagcacacgtg26880
cgcgggagcagctgctttgagtgcacacactaccaatcgttcctggaatacagggaggtg26940
gtggacggcctggagaaggccatctacaagggcccaggcagcgaagcagggccctgactg27000
cccccccggcccccctctcgggctctctcacccagcctggtactgaaggtgccagacgtg27060
ctcctgctgaccttctgcggctccgggctgtgtcctaaatgcaaagcacacctcggccga27120
ggcctgcgccctgacatgctaacctctctgaactgcaacactggattgttcttttttaag27180
actcaatcatgacttctttactaacactggctagctatattatcttatatactaatatca27240
tgttttaaaaatataaaatagaaattaagaatctaaatatttagatataactcgacttag27300
tacatccttctcaactgccattcccctgctgcccttgacttgggcaccaaacattcaaag27360
ctccccttgacggacgctaacgctaagggcggggcccctagctggctgggttctgggtgg27420
cacgcctggcccactggcctcccagccacagtggtgcagaggtcagccctcctgcagcta27480
ggccaggggcacctgttagccccatggggacgactgccggcctgggaaacgaagaggagt27540
cagccagcattcacacctttctgaccaagcaggcgctggggacaggtggaccccgcagca27600
gcaccagcccctctgggccccatgtggcacagagtggaagcatctccttccctactcccc27660
actgggccttgcttacagaagaggcaatggctcagaccagctcccgcatccctgtagttg27720
cctccctggcccatgagtgaggatgcagtgctggtttctgcccacctacacctagagctg27780
tccccatctcctccaaggggtcagactgctagccacctcagaggctccaagggcccagtt27840
cccaggcccaggacaggaatcaaccctgtgctagctgagttcacctgcaccgagaccagc27900
ccctagccaagattctactcctgggctcaaggcctggctagcccccagccagcccactcc27960
tatggatagacagaccagtgagcccaagtggacaagtttggggccacccagggaccagaa28020
acagagcctctgcaggacacagcagatgggcacctgggaccacctccacccagggccctg28080
ccccagacgcgcagaggcccgacacaagggagaagccagccacttgtgccagacctgagt28140
ggcagaaagcaaaaagttcctttgctgctttaatttttaaattttcttacaaaaatttag28200
gtgtttaccaatagtcttattttggcttatttttaa28236
<210>2
<211>2434
<212>prt
<213>homosapiens
<400>2
metproglualaproproleuleuleualaalavalleuleuglyleu
151015
valleuleuvalvalleuleuleuleuleuarghistrpglytrpgly
202530
leucysleuileglytrpasnglupheileleuglnproilehisasn
354045
leuleumetglyaspthrlysgluglnargileleuasnhisvalleu
505560
glnhisalagluproglyasnalaglnservalleuglualaileasp
65707580
thrtyrcysgluglnlysglutrpalametasnvalglyasplyslys
859095
glylysilevalaspalavalileglngluhisglnproservalleu
100105110
leugluleuglyalatyrcysglytyrseralavalargmetalaarg
115120125
leuleuserproglyalaargleuilethrilegluileasnproasp
130135140
cysalaalailethrglnargmetvalaspphealaglyvallysasp
145150155160
lysvalthrleuvalvalglyalaserglnaspileileproglnleu
165170175
lyslyslystyraspvalaspthrleuaspmetvalpheleuasphis
180185190
trplysaspargtyrleuproaspthrleuleuleugluglucysgly
195200205
leuleuarglysglythrvalleuleualaaspasnvalilecyspro
210215220
glyalaproasppheleualahisvalargglysersercyspheglu
225230235240
cysthrhistyrglnserpheleuglutyrarggluvalvalaspgly
245250255
leuglulysalailetyrlysglyproglyserglualaglyprothr
260265270
thrglyalaglyalacysalaglyalaalathrcysthrcysalagly
275280285
thrcysthrglythrcysalacyscyscysalaglyglyalathrgly
290295300
glyalaglythrglycysalaglythrglyglythrglyalaglyala
305310315320
thrcysthrcysalaglycysthrthralacysthrglycysalagly
325330335
cyscysthrthrglyalacyscysthrcyscysthrglyglyglycys
340345350
thrcysalaalaglycysglyalathrcyscysthrcyscysthrgly
355360365
cyscysthrcysalacyscyscysthrcyscyscysalaalaglythr
370375380
alaglycysthrglyglyglyalacysthralacysalaglyglycys
385390395400
alathrglythralacyscysalacyscysalathrglycyscysala
405410415
glyglycysthralaalathralaalathrthrthrthrthrglyala
420425430
glythrthrthrthrthrthrthralathralaglyalaalaalacys
435440445
alaglyglyglythrthrthrcysalathrcysalathrglythrthr
450455460
thrcyscyscysalaglyglycysthrthrglythrcysthrcysgly
465470475480
alaalathrthrcyscysthrglyglyglycyscysalathrthrcys
485490495
alaglyglycysthrthrthrglythrcyscyscysalathralathr
500505510
thrthrthrthrcyscysthrcysthrthrthrthrthrglythrala
515520525
alathrthrthrthralacysthrthrcysalaglyglyalacysala
530535540
alaalaalathrthralacysalathrcyscyscysthrthrthrcys
545550555560
thrthrthralaalacysalaalaalaalaalacysalacysthrcys
565570575
thralacysalathrthrcysthrthrthrglycysalathralacys
580585590
thrthrthrthrcysalathralathralaalaalaalaalacysala
595600605
cysalathrcyscysthralathrcysthrthrcyscysthrcyscys
610615620
cysalathralacysthrthrthrglythralathralaglyglyala
625630635640
alaalathrthrglythrthrthrcyscyscysthrcysalathrthr
645650655
cysthrthralathrthrglythrthrthrcysthralaglyglyala
660665670
glythrthrthrthrglythrthrthrthrcysalathralathrala
675680685
thrthrglyalathrthralathralaalathrthrthrthrthrala
690695700
alacyscysalathrthralaglythralaalacyscysglyglythr
705710715720
alathrthrthrthrthrthrthrthrthrthralaalaalaglyala
725730735
glyalacysalaglycysglythrcysthrthrglycysthrcysthr
740745750
thrthrthrglycyscyscysalaglyglycysthrglyglyalagly
755760765
thrglycysalaglythrglyglycysalacysalaalathrcysala
770775780
glyglyalacysthrcysalacysthrglycysalacyscyscysthr
785790795800
thrglyalaalacysthrcyscyscysglyglyglycysthrcysala
805810815
alaglythralaalathrthrcysthrcyscysalaalacyscysthr
820825830
cysalaglycyscyscyscyscysthralaalaalaglycysalacys
835840845
thrglyalaglyalathrthralacysalaglyglycysalathrgly
850855860
alaglythrcysalacyscysalacysglycyscyscysglyglycys
865870875880
cysalathralaalacysthrthrglythralathrthrthrthrala
885890895
cysalaalaglythralaalaalaalacysthrglyglyglyalaala
900905910
glyalaalaglyalacysalaalathrthrglyglyalaalaalathr
915920925
thrglythrcysthrglythrcysalathrthrthralacyscysala
930935940
glythrthrthrglythrthrglyglycysalaalaalathrthrthr
945950955960
thralathrglyalaalathralathralacyscysalathrcysthr
965970975
thralathralaalathrcysthrcysthralaglyalaglyglythr
980985990
alathralathrcyscysthrthrthrthrthralathralaglythr
99510001005
alacysalaalathrglythrthrthrthrthralaalaglythr
101010151020
glycyscysalaalaglythralacysalathrglythrthrthr
102510301035
alathrthralaalacysalaglyalacyscysalaglyalaala
104010451050
thralacyscysthrthrthralaglycyscysalaglythrcys
105510601065
thrglythralacyscysalathralathralaalaalaalaala
107010751080
thralaalaglyalaalaglycyscysalaalaalaalaalagly
108510901095
thralacysalaalaalaalaalaglythrthralacysglycys
110011051110
thrthralaalathralaalathrglyalaalathrglythrthr
111511201125
thrcysalaglycysalacysthrthrthrcysthrthrcysthr
113011351140
cysthrthrcysalaglyglythralathrthralathrthrthr
114511501155
alaglyalathralathrthrcysalaalathrglyalaalathr
116011651170
glythrcysthralathrthralathrthrthralaalacysthr
117511801185
thrcysalacysthrthralaglythralathrglyalacysthr
119011951200
cysthrglyalaglyglythrthrglyalaalaalaglythrthr
120512101215
alacysthrglyalaalaalaalacysalathrcysthrthrgly
122012251230
lysalaalaglycysthrthrthrthrthrthralaglyglycys
123512401245
cysalaalathralathralathrthralathrcysalaalaala
125012551260
cysglycysalaalathrthralaalacysalaalaalaalacys
126512701275
alacysalaalacysthrcysalacysthralaglythrthrthr
128012851290
alathralathralaalaglyalaglycysthralaglythrthr
129513001305
cysthrcysalacysthrthrthrthrthrthrthrthrthrthr
131013151320
alacysthrcysthralathrthrthrthralathralathrala
132513301335
thrthrthrthrthrthrcysthrthrthrthrcysthrthrthr
134013451350
thrcysthrthrthrthrcysthrthrthrthrthrthrthrthr
135513601365
thrthrthrthrthrthrthrthrthrthrthrthrthrthrcys
137013751380
thrglythralaglyalaglyalacysalaalaglyglythrcys
138513901395
thrthrglycysthrglythrglythrthrglycyscyscysala
140014051410
glyglycysthrglyglythrcysthrthrglyalaalacysthr
141514201425
cyscysthralaglycyscysthrcysalaalaglythrglyala
143014351440
thrcyscysalacysthrthralacyscysthrthrglyglycys
144514501455
cysthrcyscyscysalaalaalaglythrglycysthrglygly
146014651470
glyalathrthralacysalaglyglythrglythrglyalagly
147514801485
cyscysalacyscysalacyscyscysalaglycyscyscysthr
149014951500
alathrthrthrthralathralathrthrthrthrthralathr
150515101515
cysthrglyalaalacysthrglythrglythrthrthrcysthr
152015251530
glyglycysalaalaalathrthrglyglyalacysalaalagly
153515401545
thrthralaalaglyglyglythrcysalacysthrthrglycys
155015551560
thrcyscysalathrglythrglyalaglyglyglyglyalaala
156515701575
glyglycysthrthrthrcysthralacyscysthrglythrala
158015851590
thrthrthrglythrglyglyalathralaalaglyalacyscys
159516001605
thrthrthralacysglyalacysthrthrglythrthrthrthr
161016151620
glycyscyscysthrglyalathrglyglyalathrglyalacys
162516301635
thralacysalathrglythrthrthrcyscysalaglythrgly
164016451650
alaglycysthrcysalaglycysthrglyglythrthrglycys
165516601665
thrthrthrthrglythrglyglyglythrcyscyscysthrgly
167016751680
alaglyglycysalacysthrglycysglyalaglycyscyscys
168516901695
cysthrglyalathrglyglyglyglycysalaglyglyalaala
170017051710
glycyscysthralaalaalaglythrthrcyscysalaglygly
171517201725
glyalacyscysglythrglyglyglyglyalaglythrthrgly
173017351740
alaglyglyglyglycysthrglyglyalaglyglyglyglygly
174517501755
alaalaglyalaglyalaglyalaglyglycyscysthrglycys
176017651770
alaglycysglyglyglyglyalathrcyscysalacysalagly
177517801785
glythrglyglyalaglyglyalaglyglycyscysalaglyala
179017951800
cysthrthrglyalaalaglyglyglyglyglythrglythrcys
180518101815
thrcyscysthrthrcysalaalaglyalaglyalaalaalagly
182018251830
thralaglyglyglyalaalaglythralathrglyglyalagly
183518401845
glythrglyalathralaglyglyalaalaglyglyglyalagly
185018551860
alaglyglycyscysthrglyalaglyalaglyglyalaglycys
186518701875
thralaglythrthrthrglyglyglyglyalaglyalathrcys
188018851890
thrcysalaalaglythralathrcyscyscysalaalaalagly
189519001905
glyalaglycyscysalacysthrglyalathrglythrthrcys
191019151920
cysalaalaalathrthralathrcyscysthrthrglyglythr
192519301935
alaalaalaglythrcysalacysalathrcysalaalacysala
194019451950
alaglyglyalaalaglyglyalaalaglycysalaglyalacys
195519601965
alaglyalaglyglycysglycysalacysthrthrglythrthr
197019751980
cyscysthrthrglycysalaglythrthrthrthrglythrthr
198519901995
thrthrglyalathrglythrglycysthrglyalathrthrala
200020052010
alaalaglythralaglycysglycysalaglyalaglyglygly
201520202025
cysalathrglyalathrcysalaglycysalaglyglyglycys
203020352040
thrcyscysalaglythralaalaglyalacysglycysthrcys
204520502055
cyscysalacysglyglyglyalaglyglyalaglycysalaala
206020652070
glyalaalacysalacysalaglyalaalacysalaglyalagly
207520802085
glyglyglyglycysalaalaglyalacysalaglycysthrcys
209020952100
cysalacyscysalaglyglyalaglythrcysalaglyglyala
210521102115
glythrglyalaalathrcyscyscyscysthrcysthrglygly
212021252130
glyalaalacysglyalaglyglycysalacysthralaglygly
213521402145
alaalaglyalaalaglyalaalacysthrthrcyscysalagly
215021552160
cyscyscysalaglyglyalaglyglythralaglyglythrala
216521702175
glycysglythrthrcysalaglyalaalaglyalaalaglycys
218021852190
cysthrglyglyglyglycysglyglyglycysglycysglygly
219522002205
thrglyglycysglycysalacysglycyscysthrglythrala
221022152220
alathrcyscyscysalaglycysalacysthrthrthrglygly
222522302235
glyalaglyglycyscysglyalaglyglycysglyglyglycys
224022452250
glyalaalathrcysalacysglyalaglyglythrcysalagly
225522602265
glyalaglyalathrcysalaalaglyalacyscysalathrcys
227022752280
cysthrglyglycysthralaalacysalacysglyglythrgly
228522902295
alaalaalacyscyscyscysalathrcysthrcysthralacys
230023052310
thralaalaalaalaalathralacysalaalaalaalaalaala
231523202325
thrthralaglycyscysglyglyglycysglythrthrglythr
233023352340
glyglythrglyglyglycysalacyscysthralathralagly
234523502355
thrcyscyscysalaglycysthralacysthrthrglyglygly
236023652370
alaglyglycysthrglyalaglyglycysalaglyglyalagly
237523802385
alaalathrglyglycysalathrglyalaalacyscyscysgly
239023952400
glyglyglyglyalacysglyglyalaglycysthrthrglycys
240524102415
alaalathrglyalaglycyscysglyalaglyalathrthrala
242024252430
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