或1.65mg)和AgN033.I?3.7mg(例如3.I?3.4g或3.4?3.7g或3.Ig或3.4g或3.7g)。所述篩選培養(yǎng)基I的溶劑可為水。所述篩選培養(yǎng)基I的PH值可為5.4?6.0 (例如5.4?5.7或5.7?6.0或5.4或5.7或6.0)。
[0028]所述步驟(5)中，每IL所述篩選培養(yǎng)基I可由如下組分組成:MS鹽4.33g、煙酸
1.3mg、VB1.25mg、VBe0.25mg、泛酸|丐0.25mg、鹽酸硫胺0.5Omg、鹿糖30.0g、L-脯氨酸 I.38g、
2.4-D0.50mg、6-BA 0.0lOmg、植物凝膠3.5g、特美汀10mg、雙丙氨膦I.5mg和AgN033.4g，其余為水。所述篩選培養(yǎng)基I的pH值具體可為5.7。
[0029]所述步驟(5)中，每IL所述篩選培養(yǎng)基Π可包括:MS鹽3.90?4.80g(例如3.90?4.338或4.33?4.8(^或3.9(^或4.338或4.8(^)、煙酸1.2?1.411^(例如1.2?1.311^或1.3?
1.4mg或1.2mg或1.3mg或1.4mg)、VB1.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、VB60.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、泛酸|丐0.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或
0.23mg或0.25mg或0.28mg)、鹽酸硫胺0.45?0.55mg(例如0.45?0.50mg或0.50?0.55mg或0.45mg或0.50mg或0.55mg)、蔗糖27.0?33.0g(例如27.0?30.0g或30.0?33.0g或27.0g或30.(^或33.(^)、1^-脯氨酸1.24?1.528(例如1.24?1.388或1.38?1.528或1.248或1.388或I.52g) N 2,4-D 0.45 ?0.55mg (例如0.45 ?0.50mg 或 0.50 ?0.55mg 或 0.45mg 或 0.50mg 或0.55mg) N 6-BA 0.009?0.01 Img (例如0.009?0.0I Omg或0.010 ?0.01 Img或0.009mg或0.0lOmgS0.01 lmg)、植物凝膠3.15?3.85g(例如3.15?3.5g或3.5?3.85g或3.15g或3.5g或3.85g)、特美汀90?I 1mg(例如90?10mg或 100?I 1mg或90mg或10mg或I 1mg)、雙丙氛勝2.7?3.3mg(例如2.7?3.0mg或3.0?3.3mg或2.7mg或3.0mg或3.3mg)和AgN〇33.1?3.7mg(例如3.1?3.4g或3.4?3.7g或3.1g或3.4g或3.7g)。所述篩選培養(yǎng)基Π的溶劑可為水。所述篩選培養(yǎng)基Π的pH值可為5.4?6.0(例如5.4?5.7或5.7?6.0或5.4或5.7或6.0)。
[0030]所述步驟(5)中，每IL所述篩選培養(yǎng)基Π可由如下組分組成:MS鹽4.33g、煙酸
1.3mg、VB1.25mg、VBe0.25mg、泛酸|丐0.25mg、鹽酸硫胺0.5Omg、鹿糖30.0g、L-脯氨酸 I.38g、
2.4-D0.50mg、6-BA 0.0lOmg、植物凝膠3.5g、特美汀10mg、雙丙氨膦3.0mg和AgN033.4g，其余為水。所述篩選培養(yǎng)基Π的pH值具體可為5.7。
[0031]所述步驟(6)中，每IL所述恢復(fù)培養(yǎng)基Π可包括:MS鹽3.90?4.80g(例如3.90?4.338或4.33?4.8(^或3.9(^或4.338或4.8(^)、煙酸1.2?1.411^(例如1.2?1.311^或1.3?
1.4mg或1.2mg或1.3mg或1.4mg)、VB1.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、VB60.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、泛酸|丐0.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、鹽酸硫胺0.45?0.55mg(例如0.45?0.50mg或0.50?0.55mg或0.45mg或0.50mg或0.55mg)、蔗糖27.0?33.0g(例如27.0?30.0g或30.0?33.0g或27.0g或30.(^或33.(^)、1-脯氨酸1.24?1.523(例如1.24?1.383或1.38?1.523或1.243或1.388或 I.52g)、6_BA 3.15?3.85mg(例如3.15?3.5mg或3.5?3.85mg或3.15mg或3.5mg或
3.85mg)、毒賽定0.9?I.Img(例如0.9?I.0mg或I.0?I.Img或0.9mg或I.0mg或I.lmg)、植物凝膠3.15?3.858(例如3.15?3.53或3.5?3.853或3.153或3.53或3.853)、特美汀90?
11mg(例如90?10mg或 100?I 1mg或90mg或10mg或I 1mg)、雙丙氧鱗I.35?I.65mg(例如1.35?1.5mg或1.5?1.65mg或1.35mg或1.5mg或1.6511^)。所述恢復(fù)培養(yǎng)基11的溶劑可為水。所述恢復(fù)培養(yǎng)基Π的pH值可為5.4?6.0(例如5.4?5.7或5.7?6.0或5.4或5.7或6.0)。
[0032]所述步驟(6)中，每IL所述恢復(fù)培養(yǎng)基Π可由如下組分組成:MS鹽4.33g、煙酸1.311^、￥810.2511^、￥860.2511^、泛酸|150.2511^、鹽酸硫胺0.5011^、庶糖30.(^、1^-脯氨酸1.388、6-BA 3.511^、毒莠定1.011^、植物凝膠3.58、特美汀10mg、雙丙氨膦1.5mg，其余為水^所述恢復(fù)培養(yǎng)基Π的pH值具體可為5.7。
[0033]所述步驟(7)中，每IL所述分化培養(yǎng)基可包括al)或a2):
[0034]&1)15鹽3.90?4.8(^(例如3.90?4.338或4.33?4.8(^或3.9(^或4.338或
4.80g)、煙酸I.2?I.4mg(例如 I.2?I.3mg或I.3?I.4mg或 I.2mg或I.3mg或 I.4mg)、VB1.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、VBe0.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、泛酸1^0.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、鹽酸硫胺0.45?0.55mg(例如0.45?0.5mg或0.5?0.55mg或0.45mg或0.5mg或0.55mg)、庶糖 10?15g(例如 10.0?13.0g或 13.0?15.0g或 10.0g或13.0g或 15.0g)、葡萄糖 15?20g(例如15?188、18?2(^或153或188或2(^)、1-脯氨酸0.63?0.778(例如0.63?0.78或0.7?0.778或0.638或0.78或0.778)、植物凝膠3.15?3.858(例如3.15?3.58或3.5?3.858或3.158或3.58或3.853)、酪蛋白水解物0.18?0.223(例如0.18?0.21^或0.2?0.221^或0.181^或0.2mg或0.22mg)、甘氧酸0.036?0.044g(例如0.036?0.04g或0.04?0.044g或0.036g或0.04g或0.044g)、肌醇0.09?0.Ilg(例如0.09?0.1Omg或0.10?0.1111^或0.0911^或0.1mg或0.1 lmg)和雙丙氨膦2.7?3.3mg(例如2.7?3.0mg或3.0?3.3mg或2.7mg或3.0mg或3.3mg)；
[0035]a2)蔗糖 27.0?33.0g (例如27.0?30.0g 或30.0 ?33.0g 或 27.0g 或30.0g 或33.(^)、1-脯氨酸0.63?0.778(例如0.63?0.78或0.7?0.778或0.638或0.78或0.778)、植物凝膠 3.15?3.858(例如3.15?3.58或3.5?3.858或3.158或3.58或3.858)、醫(yī)032547?3113mg(例如 2547 ?2830mg 或 2830 ?3113mg 或 2547mg 或 2830mg 或 3113mg)、(NH4)2S04417 ?509mg(例如417?463mg或463?509mg或417mg或463mg或509mg)、KH2P04360?440mg(例如360?400mg或400?440mg或360mg或400mg或440mg)、MgSCU.7H2O 167?204mg(例如 167?185mg或 185?204mg或 167mg或 185mg或204mg)、CaCl2.2H2O 149?183mg(例如 149?166mg或 166?18311^或14911^或16611^或18311^)、MnS04.H2O 6.8?8.4mg(例如6.8?7.6mg或7.6?8.4mg或6.8mg或7.6mg或8.4mg)、ZnS〇4.7H2O I.8?2.2mg(例如 I.8?2mg或2?2.2mg或
I.8mg或2mg或2.2mg)、Η3Β〇32.7?3.3mg(例如2.7?3.0mg或3.0?3.3mg或2.7mg或3.0mg或3.3mg)、KI 0.68?0.83mg(例如0.68?0.75mg或0.75?0.83mg或0.68mg或0.75mg或0.83mg)、Na2Mo〇4.2H2O0.23?0.28mg(例如0.23?0.25mg或0.25?0.28mg或0.23mg或0.25mg或0.28mg)、CuS〇4.5H2O 0.023?0.028mg(例如0.023?0.025mg或0.025?0.028mg或0.023mg或0.025mg或0.028mg)、CoCl2.6H2O 0.023?0.028mg(例如0.023?0.025mg或0.025?0.028mg或0.023mg或0.025mg或0.028mg)、乙二胺四乙酸鈉鐵鹽32.9?40.1mg(例如32.9?36.6mg或36.6?40.1mg或32.9mg或36.6mg或40.1mg)、氣化膽喊0.088?0.108mg(例如0.088?0.098mg或0.098?0.108mg或0.088mg或0.098mg或0.108mg)、VB20.044?0.054mg(例如0.044?0.049mg或0.049?0.054mg或0.044mg或0.049mg或0.054mg)、D_生物素0.09 ?0.1 Img (例如0.09 ?0.1 Omg或 0.1O ?0.1 Img或 0.09mg或 0.I Omg或 0.1 lmg)、煙酸0.18?0.22mg(例如0.18?0.2mg或0.2?0.22mg或0.18mg或0.2mg或0.22mg)、葉酸0.0437?0.0534mg(例如0.0437?0.0485mg或0.0485?0.0534mg或0.0437mg或0.0485mg或0.0534mg) N VB1.425?0.519mg(例如0.425?0.472mg或0.472?0.519mg或0.425mg或0.472mg或0.519mg)、D-泛酸I丐0.09?0.1 Img(例如0.09?0.1Omg或0.10?0.1 Img或0.09mg或0.1Omg或0.1 lmg)、VB60.18?0.22mg(例如0.18?0.2mg或0.2?0.22mg或0.18mg或0.2mg或0.22mg)、VBi2O-OO 14?0.0017mg(例如0.0014?0.0015mg或0.0015?0.0017mg或0.0014mg或0.0015mg或0.0017mg)、對氛基苯甲酸0.044?0.054mg(例如0.044?0.049mg或0.049?0.054mg或0.044mg或0.049mg或0.054mg)、酪蛋白水解物0.18?0.22g(例如0.18?0.2mg或0.2?0.22mg或0.18mg或0.2mg或0.22mg)、甘氛酸0.036?0.044g(例如0.036?0.04g或0.04?0.044g或0.036g或0.04g或0.044g)、肌醇0.09?0.I Ig(例如0.09?0.1mg或0.10?0.1 Img或0.09mg或0.1Omg或0.1 lmg)和雙丙氨勝2.7?3.3mg(例如2.7?3.0mg或3.0 ?3.3mg或 2.7mg或 3.0mg或 3.3mg)。
[0036]所述分化培養(yǎng)基的溶劑可為水。所述分化培養(yǎng)基的pH值可為5.5?6.1(例如5.5?5.8或5.8?6.1或5.5或5.8或6.1或5.7)。
[0037]所述步驟(7)中，每IL所述分化培養(yǎng)基具體可由如下Al)或A2)中各個(gè)組分組成:
[0038]Al )MS鹽4.33g、煙酸I.3mg、VB1.25mg、VB60.25mg、泛酸鈣0.25mg、鹽酸硫胺0.