(S)-苯基乙?；状际怯袡C(jī)合成中有價值的手性單元并且可用于合成精細(xì)化學(xué)品和藥物。根據(jù)迄今的現(xiàn)有技術(shù)，并不已知能夠借助不對稱合成由非手性的廉價化合物以>89%ee的光學(xué)純度生成(S)-苯基乙酰基甲醇(S)-PAC的方法。但是，在制備精細(xì)化學(xué)品或藥物時，高的光學(xué)純度是極其重要的。根據(jù)現(xiàn)有技術(shù)已知制備(S)-苯基乙?；状嫉母鞣N方法。一方面，已知化學(xué)合成?；诨瘜W(xué)不對稱合成的(S)-PAC制備方法生成68%或86%的ee。這些方法描述在出版物Davis,FranklinA.;Sheppard,AureliaC.;Lal,G.SankarTetrahedronLetters,1989,第30卷,7第779-782頁和Adam,Waldemar;Fell,RainerT.;Stegmann,VeitR.;Saha-Moeller,ChantuR.JournaloftheAmericanChemicalSociety,1998,第120卷,4第708-714頁中。此外，還有僅作為副產(chǎn)物產(chǎn)生(S)-PAC且(R)-PAC以對映體過量形式存在的方法，這例如通過下列反應(yīng)，例如描述在出版物Toukoniitty,Esa;Maeki-Arvela,Paeivi;Kuzma,Marek;Villela,Alexandre;KalantarNeyestanaki,Ahmad;Salmi,Tapio;Sjoeholm,Rainer;Leino,Reko;Laine,Ensio;Murzin,DmitryYu,JournalofCatalysis,2001,第204卷,2第281-291頁中的1-苯基丙-1,2-二酮的還原和描述在Fleming,StevenA.;Carroll,SeanM.;Hirschi,Jennifer;Liu,Renmao;Pace,J.Lee;Redd,J.TyTetrahedronLetters,2004,第45卷,17第3341-3343頁中的由苯甲醛起始的合成和描述在Brussee,J.;Roos,E.C.;Gen,A.VanDerTetrahedronLetters,1988,第29卷,35第4485-4488頁中的2-羥基-2-苯基乙腈的反應(yīng)。此外，描述了手性單元1-苯基丙-1,2-二醇可以氧化成(S)-PAC的合成。(S)-PAC以91%的對映體過量產(chǎn)生，如由Zi-QiangRong,Hui-JiePan,Hai-LongYan和YuZhaoOrganicLetters,2014,16(1),第208–211頁描述，或69%的對映體過量，如由WaldemarAdam,ChantuR.Saha-Moeller和Cong-GuiZhaoJournalofOrganicChemistry,64(20),7492-7497;1999描述，但是此外被必須復(fù)雜分離出的區(qū)域異構(gòu)體污染。此外，已知描述在álvaroGómezBaraibar于2013年的題為“Developmentofabiocatalyticproductionprocessfor(S)-alpha-hydroxyketones”博士論文中的酶促不對稱合成。如果將根據(jù)該博士論文的這種在大腸桿菌中異源表達(dá)的酶用于在全細(xì)胞中合成，(S)-PAC的光學(xué)純度為~43%ee。(S)-PAC的這個唯一的酶促不對稱合成描述在由苯甲醛和乙醛或苯甲醛和丙酮酸起始的碳連接反應(yīng)中。該反應(yīng)由酶，即來自巴氏醋桿菌的丙酮酸脫羧酶的變體ApPDC-E469G催化，其中在位置號469中的谷氨酸被甘氨酸替換。在此通過分離的酶實(shí)現(xiàn)的最高對映體過量為89%，如由出版物RotherNeeGocke,Doerte;Kolter,Geraldine;Gerhards,Tina;Berthold,CatrineL.;Gauchenova,Ekaterina;Knoll,Michael;Pleiss,Juergen;Mueller,Michael;Schneider,Gunter;Pohl,Martina,ChemCatChem,2011,第3卷,10第1587-1596頁描述。由現(xiàn)有技術(shù)出發(fā)，本發(fā)明的目的是提供不對稱合成(S)-苯基乙?；状嫉奶娲悦复俜椒?，其實(shí)現(xiàn)(S)-苯基乙酰基甲醇的高對映體過量。在用全細(xì)胞制備時，對映體過量應(yīng)大于43%。此外，不應(yīng)形成副產(chǎn)物和區(qū)域異構(gòu)體。在此，應(yīng)能夠使用非手性的廉價反應(yīng)物。應(yīng)實(shí)現(xiàn)(S)-苯基乙酰基甲醇的不對稱合成。應(yīng)防止手性產(chǎn)物混合物的昂貴分離。應(yīng)提供可以用于由苯甲醛和丙酮酸或乙醛制備(S)-苯基乙?；状嫉拿?，以及編碼該酶的DNA。此外應(yīng)提供制備該酶的方法。應(yīng)克服現(xiàn)有技術(shù)中存在的缺點(diǎn)和問題。應(yīng)提供制備(S)-苯基乙?；状嫉姆椒ǎ渖踔猎谑褂么旨?xì)胞提取物（Rohzellextrakt）或全細(xì)胞時實(shí)現(xiàn)高對映體過量。應(yīng)累積地克服現(xiàn)有技術(shù)的缺點(diǎn)。由權(quán)利要求1的上位概念和從屬權(quán)利要求出發(fā)，所述目的根據(jù)本發(fā)明通過在權(quán)利要求的特征部分中給出的特征來實(shí)現(xiàn)。該目的根據(jù)本發(fā)明通過裂解酶ApPDC-E469G的變體來實(shí)現(xiàn)，在該變體的位置號468中的異亮氨酸被具有相對于異亮氨酸減小的占據(jù)空間的氨基酸替換。此外，通過根據(jù)從屬權(quán)利要求將裂解酶用于使苯甲醛與丙酮酸或乙醛反應(yīng)成(S)-苯基乙?；状迹瑢?shí)現(xiàn)該目的。通過本發(fā)明的裂解酶以及(S)-苯基乙?；状嫉闹苽浞椒ǎF(xiàn)在可以在使用全細(xì)胞時以93%ee和在使用粗細(xì)胞提取物時以85%ee制備(S)-苯基乙?；状?。不形成副產(chǎn)物，特別是不產(chǎn)生區(qū)域異構(gòu)體。由于該合成用非手性反應(yīng)物也可實(shí)現(xiàn)，其是廉價的?？梢悦獬龑τ丑w的分離。在制備(S)-苯基乙?；状紩r，也可以通過粗細(xì)胞提取物或全細(xì)胞實(shí)現(xiàn)高對映體過量。累積地消除根據(jù)現(xiàn)有技術(shù)描述的所有缺點(diǎn)。本發(fā)明的有利的擴(kuò)展實(shí)施方案在從屬權(quán)利要求中給出。根據(jù)本發(fā)明提供裂解酶，其中在相對于巴氏醋桿菌野生型修飾的蛋白ApPDC-E469G中在位置號468中的異亮氨酸被比異亮氨酸較不占空間的氨基酸置換。該裂解酶對于在制備(S)-苯基乙?；状紩r提高立體選擇性而言具有有利影響。優(yōu)選地，可以提及滿足這些要求的下列裂解酶：-根據(jù)序列號1在位置號468中具有甘氨酸的ApPDC-E469G-I468G-根據(jù)序列號2在位置號468中具有丙氨酸的ApPDC-E469G-I468A-根據(jù)序列號3在位置號468中具有纈氨酸的ApPDC-E469G-I468V。為了改進(jìn)對映體過量，也可以在制備(S)-苯基乙?；状紩r使用酶ApPDC-E469G-I468L、ApPDC-E469G-I468T、ApPDC-E469G-I468C或ApPDC-E469G-I468S。此外，提供編碼所述酶的本發(fā)明的脫氧核糖核酸。根據(jù)本發(fā)明，這是編碼酶ApPDC-E469G的變體的脫氧核糖核酸，其在位置號1402至1404中編碼相對于異亮氨酸具有減小的占據(jù)空間的氨基酸。對于在所述位置中編碼氨基酸甘氨酸的根據(jù)序列號1的實(shí)例，可以在位置號1402-1404中例如存在核酸GGT。例如，描述了根據(jù)序列號4的編碼根據(jù)序列號1的在位置號468中具有甘氨酸的酶ApPDC-E469G-I468G的脫氧核糖核酸。對于本發(fā)明的根據(jù)序列號2的在位置號468中具有丙氨酸的酶ApPDC-E469G-I468A和根據(jù)序列號3的在位置號468中具有纈氨酸的酶ApPDC-E469G-I468V，可以通過替換位置號1402-1404中的相應(yīng)核苷酸提供編碼其的脫氧核糖核酸。編碼本發(fā)明的酶的DNA可以根據(jù)本領(lǐng)域技術(shù)人員已知的方法通過定向或不定向的誘變來產(chǎn)生。在此，優(yōu)選的是定向誘變。這些方法是本領(lǐng)域技術(shù)人員已知的。在具體的描述部分中，具體公開本發(fā)明的一個實(shí)施方案的制備實(shí)例。這種方法方式原則上也可以用于所有其它公開的脫氧核糖核酸和酶，以使得可以以類似途徑制備所有的本發(fā)明的酶和脫氧核糖核酸。所述脫氧核糖核酸可以連接到載體，優(yōu)選質(zhì)粒中。作為空載體，可以例如使用pET-20b(+)、pET-21a-d(+)、pET-22b(+)、pET-23a-d(+)、pET-24a-d(+)、pET-25b(+)、pET-26b(+)、pET-27b(+)、pET-28a(+)、pET-29a-c(+)、pET-30a-c(+)、pET-31b(+)、pET-34b(+)、pET-35b(+)、pET-36b(+)、pET-37b(+)、pET-38b(+)，向其中連接相應(yīng)的本發(fā)明DNA。替代地，也可以將該脫氧核糖核酸連接到基因組中。連接的脫氧核糖核酸是DNA序列，其編碼酶ApPDC-E469G的變體并在位置號1402-1404中編碼具有相對于異亮氨酸減小的占據(jù)空間的氨基酸。這些脫氧核糖核酸也可以編碼酶ApPDC-E469G-I468L、ApPDC-E469G-I468T、ApPDC-E469G-I468C或ApPDC-E469G-I468S。優(yōu)選地，連接的脫氧核糖核酸編碼根據(jù)序列1、2和3的蛋白。根據(jù)本發(fā)明可以提供載體，其含有編碼酶ApPDC-E469G的變體并在位置號1402-1404中編碼具有相對于異亮氨酸減小或相等的占據(jù)空間的氨基酸的脫氧核糖核酸。優(yōu)選地，該載體含有根據(jù)序列號4的脫氧核糖核酸。優(yōu)選地，該載體是質(zhì)粒。圖1展示了本發(fā)明的質(zhì)粒（pET-21a(+)載體卡）。在序列號5中例如示出本發(fā)明質(zhì)粒的DNA序列，其含有根據(jù)序列號4的DNA。根據(jù)本發(fā)明，借助在位置號468中具有相對于異亮氨酸具有減小的占據(jù)空間的氨基酸的酶ApPDC-E469G的變體，優(yōu)選選自序列號1、2或3的酶，使苯甲醛與丙酮酸或與乙醛根據(jù)式(1)反應(yīng)成(S)-苯基乙?；状肌Ｊ?1)該反應(yīng)優(yōu)選在水溶液中進(jìn)行。pH值為5-9，優(yōu)選6.5-8，特別優(yōu)選6.5-7。對此，可以使用例如磷酸鉀緩沖液、HEPES、MOPS、TEA或TRIS-HCl作為緩沖液。此外，可以使用磷酸硫胺素和硫酸鎂作為輔因子。該反應(yīng)可以在體內(nèi)或在體外進(jìn)行。對于在體內(nèi)生產(chǎn)(S)-苯基乙?；状?，可以使用例如大腸桿菌、棒狀桿菌例如谷氨酸棒狀桿菌或酵母例如釀酒酵母作為生產(chǎn)生物體。為此，該生產(chǎn)生物體通過本發(fā)明的DNA或包含該DNA的載體進(jìn)行轉(zhuǎn)化。該DNA也可以在生產(chǎn)生物體中引入基因組中。所用的基因在此異源表達(dá)。對于在體外生產(chǎn)，可以使用該生產(chǎn)生物體的分離的酶或細(xì)胞提取物。典型溫度為20℃至40℃，優(yōu)選20℃至30℃，特別優(yōu)選為20℃至25℃的溫度。反應(yīng)時間可以為2h-48h，優(yōu)選6h-24h，特別優(yōu)選12h。下面介紹若干實(shí)施例，其不解釋為具有限制性。這些反應(yīng)可以以經(jīng)典的配方（Ansatz）在反應(yīng)燒瓶中在攪拌下進(jìn)行。為了能夠以高對映體過量制備(S)-PAC，通過誘變產(chǎn)生酶ApPDC-E469G的變體。變體ApPDC-E469G-I468G通過使用粗細(xì)胞提取物酶以85%ee生產(chǎn)(S)-PAC。通過使用在大腸桿菌中異源表達(dá)并用作廉價全細(xì)胞催化劑的ApPDC-E469G-I468G，可以以93%ee生成(S)-PAC。實(shí)施例1：20mM苯甲醛、400mM丙酮酸、2.5mM硫酸鎂、100μM二磷酸硫胺素、20mg/mL（濕重）ApPDC-E469G-I468G（大腸桿菌的全細(xì)胞，其中ApPDC-E469G-I468G以表達(dá)形式存在）、50mM磷酸鉀緩沖液pH6.5，25℃，反應(yīng)時間：48h。(S)-PAC的對映體純度：ee93%收率：67%。實(shí)施例2：20mM苯甲醛、400mM丙酮酸、2.5mM硫酸鎂、100μM二磷酸硫胺素、1mg/mLApPDC-E469G-I468G（大腸桿菌的粗細(xì)胞提取物，其中ApPCD-E469G-I468G以表達(dá)形式存在）、50mM磷酸鉀緩沖液pH6.5，25℃，反應(yīng)時間：48h。(S)-PAC的對映體純度：ee85%。下面示例性闡述本發(fā)明的酶和編碼其的脫氧核糖核酸的制備。所示方法原則上也可以類似地用于制備其它的本發(fā)明裂解酶和編碼其的脫氧核糖核酸。酶變體ApPDC-E469G-I468G的DNA的制備“位點(diǎn)飽和誘變”根據(jù)Reetz等人（Reetz,Kahakeaw等人2008）的變體的所謂的“位點(diǎn)飽和誘變”的方法由基因序列ApPDC-E469G（模板DNA）起始進(jìn)行，以獲得在位置號I468上的氨基酸替換。在這一方法中，使用編碼20種天然氨基酸中的12種的NDT-密碼子。聚合酶鏈反應(yīng)（PCR）在最初的步驟中，借助聚合酶鏈反應(yīng)（PCR）復(fù)制模板DNA，并在此同時通過使用所謂的簡并引物（degeneriertenPrimer）和NDT密碼子插入（eingefügt）突變。所用的引物從“EurofinsMWGOperon”公司訂購（http://www.eurofinsgenomics.eu）并具有下列序列：用于制備ApPDC-E469G-I468NDT的位點(diǎn)飽和誘變的引物正向：5′CCGTGGCTATGTCNDTGGCATCGCCATTC3′（序列號6）反向：5′GAATGGCGATGCCAHNGACATAGCCACGG3′（序列號7）。首先制備母液并隨后分為四個各為50μl的配制品。為了開始該反應(yīng)，添加1μl“KOD熱啟動聚合酶”。PCR-反應(yīng)配方：該反應(yīng)在下列條件下進(jìn)行：為了消化模板DNA，將1μl的酶DpnI（Eppendorf）添加到溶液中并在37℃下培養(yǎng)1h。整個配制品然后用“DNA純化試劑盒”（化學(xué)品列表）在進(jìn)一步轉(zhuǎn)化前進(jìn)行提純。大腸桿菌BL21-DE3和大腸桿菌DH5α的轉(zhuǎn)化菌株大腸桿菌BL21-DE3和大腸桿菌DH5α用通過“位點(diǎn)飽和誘變”制備的DNA進(jìn)行轉(zhuǎn)化。為此，向50μl感受態(tài)細(xì)胞中添加100ngDNA并在冰上培養(yǎng)30分鐘。隨后在42℃下進(jìn)行熱休克90秒。3分鐘后，向冰上添加500μlSOC培養(yǎng)基并然后將該溶液在350轉(zhuǎn)/分鐘下且在37℃下在Eppendorf恒溫混勻儀中培養(yǎng)45分鐘。在完成培養(yǎng)后，將細(xì)胞懸浮液在13000轉(zhuǎn)/分鐘下在Eppendorf臺式離心機(jī)中離心分離30秒，并將沉淀物（Pellet）接著再懸浮在100μl上清液中。濃縮至100μl的細(xì)胞懸浮液在LB瓊脂平板（具有100μg/ml氨芐青霉素）上鋪板并在37℃下倒立著培養(yǎng)16h。酶變體的表達(dá)轉(zhuǎn)化的46個單菌落從板上用牙簽挖出并在20℃和850轉(zhuǎn)/分鐘下在48孔Nerbe板（NerbePlusGmbH）的各具有1mlLB培養(yǎng)基的孔（“母板”）中分別培養(yǎng)24h。另一個孔用大腸桿菌BL21-DE3細(xì)胞接種，其事前與ApPDC-E469G-模板DNA類似地轉(zhuǎn)化。在完成培養(yǎng)后，在48孔FlowerPlates?（m2p-labs,德國）中向各1.5ml自動誘導(dǎo)培養(yǎng)基中加入10μl細(xì)胞懸浮液。該FlowerPlate在20℃和850轉(zhuǎn)/分鐘下培養(yǎng)48h。向“母板”的剩余體積（990μl）加入300μl甘油并在-80℃下儲存。細(xì)胞分解和碳連接將FlowerPlates?中表達(dá)的變體通過冷凍（48h，4℃）分解。在重新解凍后，將各500μl的細(xì)胞懸浮液轉(zhuǎn)移到96孔板的兩個孔中（雙重測定）。將該板在4000轉(zhuǎn)/分鐘下離心3分鐘并將沉淀物再懸浮到具有1mg/ml溶菌酶的420μlKpi緩沖液中。該板在20℃和400轉(zhuǎn)/分鐘下培養(yǎng)1h并隨后重新在4000轉(zhuǎn)/分鐘下離心10分鐘。將各250μl上清液移液到2mlNerbe板的各一個孔中并添加由40mM苯甲醛、400mM丙酮酸、4mM硫酸鎂和400μM二磷酸硫胺素形成的250μl反應(yīng)溶液。該板重新培養(yǎng)24h并隨后分析反應(yīng)溶液（參見HPLC分析）。HPLC分析向200μl碳連接反應(yīng)溶液加入各200μl庚烷，渦旋并隨后將各150μl的上部相轉(zhuǎn)移到HPLC小瓶中。樣品分析通過ChiralpakIC-3柱（ChiralTechnologiesInc.）在使用下列方法的情況下進(jìn)行。HPLC程序典型的保留時間和用于定量的波長DNA分離和通過DNA測序的最佳酶變體的識別將碳連接反應(yīng)中對于(S)-PAC提供最高ee值的酶的DNA由母板起始進(jìn)行測序以識別突變。為此，首先將細(xì)胞用接種環(huán)從加入有甘油的母板轉(zhuǎn)移到50mLLB培養(yǎng)基（+50μg/ml氨芐青霉素）中并在250mL錐形瓶中在37℃下培養(yǎng)。在12h培養(yǎng)后，將20mL細(xì)胞懸浮液離心分離（4000轉(zhuǎn)/分鐘，5min，4℃）。沉淀物中的細(xì)胞的DNA類似于制造商說明（QiagenN.V.）根據(jù)“QIAprep?SpinMiniprepKit”方法進(jìn)行分離。為此，將DNA的濃度設(shè)定為100ng/μl并通過LGCGenomicsGmbH公司測序。LB（溶菌肉湯）培養(yǎng)基借助QuikChange?制備變體ApPDC-E469G-I468G的替代的定向方法制備酶變體ApPDC-E469G/I468G的另一方法例如是所謂的QuikChange?-PCR法（U.S.專利號5.789.166、5.932.419、6.391.548）。在PCR的這一變體的情況中，使用在待替換的DNA三聯(lián)體密碼的位置上帶有相應(yīng)的序列修飾的引物對。為了制備酶變體ApPDC-E469G/I468G，可以使用編碼變體ApPDC-E469G的基因。這一所謂的DNA模板應(yīng)在載體（例如pET22a）中以克隆形式存在。代替在位置I468中編碼氨基酸色氨酸的三聯(lián)體密碼，必須使用在該位置上帶有編碼甘氨酸的突變的引物（即GGA、GGT、GGC或GGG）。這種QuikChange?-PCR法的所有其它參數(shù)以及所需引物的選擇可以類似于制造商說明（AgilentTechnologies,Inc.）借助“QuikChange?定點(diǎn)誘變試劑盒”的指導(dǎo)來進(jìn)行。用于制備變體ApPDC-E469G-I468G的QuikChange?-PCR法的DNA模板（ApPDC-E469G）。在序列表中，該序列以序列號8示出。序列號8在此例如對于編碼根據(jù)序列號53的待修飾的蛋白ApPDC-E469G的DNA公開。但是，根據(jù)本發(fā)明，編碼待修飾的起始蛋白的所有其它脫氧核糖核酸可以用于制備待修飾的酶。為其編碼的核苷酸是本領(lǐng)域技術(shù)人員已知的?！叭?xì)胞”形式的變體的制備為了以1L的規(guī)模在全細(xì)胞中表達(dá)酶，首先將細(xì)胞從加入有甘油的母板用接種環(huán)轉(zhuǎn)移到50mLLB培養(yǎng)基（+100μg/ml氨芐青霉素）中并在250mL錐形瓶中在120轉(zhuǎn)/分鐘和37℃下培養(yǎng)。在16h培養(yǎng)后，向1L自動誘導(dǎo)培養(yǎng)基中加入10mL培養(yǎng)物并在5L錐形瓶中在90轉(zhuǎn)/分鐘和20℃下培養(yǎng)72h。隨后通過離心（4℃，6000轉(zhuǎn)/分鐘，30min）收獲該細(xì)胞并在-20℃下儲存以進(jìn)一步使用。自動誘導(dǎo)培養(yǎng)基12g/l蛋白胨24g/l酵母提取物90mM磷酸鉀緩沖液(pH7.5)0.5g/l葡萄糖2g/l乳糖0.01g/l氨芐青霉素6.3g/l甘油“細(xì)胞提取物”形式的變體的制備將10g以1L規(guī)模培養(yǎng)的細(xì)胞（參見““全細(xì)胞”形式的變體的制備”）用冷卻到4℃的25mL破碎緩沖液（50mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂）在冰上再懸浮。隨后，將再懸浮的細(xì)胞借助超聲（SD14超聲波發(fā)生器（HielscherUltrasonicsGmbH），4x2min超聲處理，各1分鐘來自冰的冷卻）進(jìn)行破碎。為了分離細(xì)胞碎片，將該溶液離心分離（45min，18000轉(zhuǎn)/分鐘，4℃）并將上清液（“細(xì)胞提取物”）轉(zhuǎn)移到新容器中?！胺蛛x的酶”形式的變體的制備為了借助固定化金屬離子親合色譜法和尺寸排阻色譜法提純ApPDC變體，使用“AmershamBioscience”公司的“?KTA?purifier”，以尤其檢測蛋白-UV吸收（280nm）以及導(dǎo)電性并調(diào)節(jié)流速。為了提純，將25mL制備的細(xì)胞提取物（參見““細(xì)胞提取物”形式的變體的制備”）以3mL/min的流速施加到體積為60mL的柱Ni-NTA-Superflow（QiagenN.V.）上，其事前用180mL上樣緩沖液進(jìn)行平衡。隨后，繼續(xù)用上樣緩沖液以5ml/min的流速沖洗，以除去不結(jié)合或非常弱地結(jié)合在柱材料上的蛋白。在UV吸收（280nm）重新達(dá)到穩(wěn)定基線后，漂洗緩沖液（50mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂、50mM咪唑）以5mL/min流速用于洗脫弱結(jié)合在柱材料上的蛋白。在重新達(dá)到穩(wěn)定UV吸收（280nm）后，以5mL/min的流速使用洗脫緩沖液（50mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂、250mM咪唑）以洗脫目標(biāo)蛋白。將IMAC的洗出液以10mL/min的流速施加到尺寸排阻色譜柱（1L柱體積，Sephadex-G25，GE-Health-care）上以進(jìn)行再緩沖，該柱事先用2L再緩沖緩沖液（10mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂）沖洗。合并具有提高的UV吸收（280nm）的級分并在結(jié)晶皿中冷凍（-20℃）。為了凍干，向冷凍的蛋白溶液施加0.22mBar的負(fù)壓3天。在本發(fā)明的另一個有利配置中，本發(fā)明的酶除了在位置號468中的根據(jù)本發(fā)明的替換之外，氨基酸色氨酸還在位置號543中被比色氨酸具有更小的占據(jù)空間的氨基酸替換。這些裂解酶此外還對在制備(S)-苯基乙?；状紩r提高立體選擇性具有有利影響。通過本發(fā)明的這一優(yōu)選的實(shí)施方案，可以實(shí)現(xiàn)98%ee的對映體過量。優(yōu)選地，可以提及滿足這些要求的下列裂解酶：-根據(jù)序列號9在位置號543中具有組氨酸的ApPCD-E469G-I468G-W543H-根據(jù)序列號10在位置號543中具有苯丙氨酸的ApPCD-E469G-I468G-W543F-根據(jù)序列號11在位置號543中具有脯氨酸的ApPCD-E469G-I468G-W543P-根據(jù)序列號12在位置號543中具有異亮氨酸的ApPCD-E469G-I468G-W543I-根據(jù)序列號13在位置號543中具有亮氨酸的ApPCD-E469G-I468G-W543L-根據(jù)序列號14在位置號543中具有甲硫氨酸的ApPCD-E469G-I468G-W543M-根據(jù)序列號15在位置號543中具有纈氨酸的ApPCD-E469G-I468G-W543V-根據(jù)序列號16在位置號543中具有丙氨酸的ApPCD-E469G-I468G-W543A-根據(jù)序列號17在位置號543中具有酪氨酸的ApPCD-E469G-I468G-W543Y-根據(jù)序列號18在位置號543中具有蘇氨酸的ApPCD-E469G-I468G-W543T-根據(jù)序列號19在位置號543中具有甘氨酸的ApPCD-E469G-I468G-W543G-根據(jù)序列號20在位置號543中具有絲氨酸的ApPCD-E469G-I468G-W543S-根據(jù)序列號21在位置號543中具有半胱氨酸ApPCD-E469G-I468G-W543C以及-根據(jù)序列號22在位置號543中具有組氨酸的ApPCD-E469G-I468A-W543H-根據(jù)序列號23在位置號543中具有苯丙氨酸的ApPCD-E469G-I468A-W543F-根據(jù)序列號24在位置號543中具有脯氨酸的ApPCD-E469G-I468A-W543P-根據(jù)序列號25在位置號543中具有異亮氨酸的ApPCD-E469G-I468A-W543I-根據(jù)序列號26在位置號543中具有亮氨酸的ApPCD-E469G-I468A-W543L-根據(jù)序列號27在位置號543中具有甲硫氨酸的ApPCD-E469G-I468A-W543M-根據(jù)序列號28在位置號543中具有纈氨酸的ApPCD-E469G-I468A-W543V-根據(jù)序列號29在位置號543中具有丙氨酸的ApPCD-E469G-I468A-W543A-根據(jù)序列號30在位置號543中具有酪氨酸的ApPCD-E469G-I468A-W543Y-根據(jù)序列號31在位置號543中具有蘇氨酸的ApPCD-E469G-I468A-W543T-根據(jù)序列號32在位置號543中具有甘氨酸的ApPCD-E469G-I468A-W543G-根據(jù)序列號33在位置號543中具有絲氨酸的ApPCD-E469G-I468A-W543S-根據(jù)序列號34在位置號543中具有半胱氨酸的ApPCD-E469G-I468A-W543C和-根據(jù)序列號35在位置號543中具有組氨酸的ApPCD-E469G-I468V-W543H-根據(jù)序列號36在位置號543中具有苯丙氨酸的ApPCD-E469G-I468V-W543F-根據(jù)序列號37在位置號543中具有脯氨酸的ApPCD-E469G-I468V-W543P-根據(jù)序列號38在位置號543中具有異亮氨酸的ApPCD-E469G-I468V-W543I-根據(jù)序列號39在位置號543中具有亮氨酸的ApPCD-E469G-I468V-W543L-根據(jù)序列號40在位置號543中具有甲硫氨酸的ApPCD-E469G-I468V-W543M-根據(jù)序列號41在位置號543中具有纈氨酸的ApPCD-E469G-I468V-W543V-根據(jù)序列號42在位置號543中具有丙氨酸的ApPCD-E469G-I468V-W543A-根據(jù)序列號43在位置號543中具有酪氨酸的ApPCD-E469G-I468V-W543Y-根據(jù)序列號44在位置號543中具有蘇氨酸的ApPCD-E469G-I468V-W543T-根據(jù)序列號45在位置號543中具有甘氨酸的ApPCD-E469G-I468V-W543G-根據(jù)序列號46在位置號543中具有絲氨酸的ApPCD-E469G-I468V-W543S-根據(jù)序列號47在位置號543中具有半胱氨酸的ApPCD-E469G-I468V-W543C。此外，根據(jù)本發(fā)明提供編碼所述酶的脫氧核糖核酸。根據(jù)本發(fā)明，這是編碼酶ApPDC-E469G的變體并在位置號1402-1404中編碼具有相對于異亮氨酸減小的占據(jù)空間的氨基酸且此外在位置號1627-1629中編碼比色氨酸較小占據(jù)空間的氨基酸的脫氧核糖核酸。對于根據(jù)序列號23的蛋白的實(shí)例，可以在相應(yīng)脫氧核糖核酸的位置號1402-1404中例如存在核酸GCC并在位置號1627-1629中存在核酸TTT。相應(yīng)的核苷酸序列在序列表中列在編號48下。編碼根據(jù)序列9至47的本發(fā)明酶的DNA可以根據(jù)本領(lǐng)域技術(shù)人員已知的方法通過定向或不定向的誘變來產(chǎn)生。在此，優(yōu)選的是定向誘變。這些方法是本領(lǐng)域技術(shù)人員已知的。在具體的描述部分中，具體公開本發(fā)明的一個實(shí)施方案的制備實(shí)例。這種方法方式原則上也可以用于所有其它公開的脫氧核糖核酸和酶，以使得可以以類似途徑制備所有的本發(fā)明的酶和脫氧核糖核酸。所述脫氧核糖核酸可以連接到載體，優(yōu)選質(zhì)粒中。作為空載體，可以例如使用pET-20b(+)、pET-21a-d(+)、pET-22b(+)、pET-23a-d(+)、pET-24a-d(+)、pET-25b(+)、pET-26b(+)、pET-27b(+)、pET-28a(+)、pET-29a-c(+)、pET-30a-c(+)、pET-31b(+)、pET-34b(+)、pET-35b(+)、pET-36b(+)、pET-37b(+)、pET-38b(+)，向其中連接相應(yīng)的本發(fā)明DNA。替代地，也可以將該脫氧核糖核酸連接到基因組中。連接的脫氧核糖核酸是DNA，其編碼酶ApPDC-E469G的變體并在位置號1402-1404中編碼具有相對于異亮氨酸減小的占據(jù)空間的氨基酸且在位置號1627-1629中編碼具有相對于色氨酸減小的占據(jù)空間的氨基酸。優(yōu)選地，連接的脫氧核糖核酸編碼根據(jù)序列9至47的蛋白。根據(jù)本發(fā)明可以提供載體，其含有編碼酶ApPDC-E469G的變體并在位置號1402-1404中編碼具有相對于異亮氨酸減小的占據(jù)空間的氨基酸且在位置號1627-1629中編碼具有相對于色氨酸減小的占據(jù)空間的氨基酸的脫氧核糖核酸。優(yōu)選地，該載體含有根據(jù)序列號48的脫氧核糖核酸。優(yōu)選地，該載體是質(zhì)粒。在序列號49中例如示出本發(fā)明質(zhì)粒的序列，其含有根據(jù)序列號48的DNA。根據(jù)本發(fā)明，還在本發(fā)明的有利配置中借助在位置號468中存在具有相對于異亮氨酸減小的占據(jù)空間的氨基酸且在位置號543中存在具有相對于色氨酸減小的占據(jù)空間的氨基酸的酶ApPDC-E469G的變體，優(yōu)選選自根據(jù)序列號9至47的酶，使苯甲醛與丙酮酸或與乙醛根據(jù)式(1)反應(yīng)成(S)-苯基乙?；状肌Ｊ?1)該反應(yīng)優(yōu)選在水溶液中進(jìn)行。pH值為5-9，優(yōu)選6.5-8，特別優(yōu)選6.5-7。對此，可以使用例如磷酸鉀緩沖液、HEPES、MOPS、TEA或TRIS-HCl作為緩沖液。此外，可以使用磷酸硫胺素和硫酸鎂作為輔因子。該反應(yīng)可以在體內(nèi)或在體外進(jìn)行。對于在體內(nèi)生產(chǎn)(S)-苯基乙酰基甲醇，可以使用例如大腸桿菌、棒狀桿菌例如谷氨酸棒狀桿菌或酵母例如釀酒酵母作為生產(chǎn)生物體。為此，該生產(chǎn)生物體通過本發(fā)明的DNA或包含該DNA的載體進(jìn)行轉(zhuǎn)化。該DNA也可以在生產(chǎn)生物體中引入基因組中。所用的基因在此異源表達(dá)。對于在體外生產(chǎn)，可以使用該生產(chǎn)生物體的分離的酶或細(xì)胞提取物。典型溫度為20℃至40℃，優(yōu)選20℃至30℃，特別優(yōu)選為20℃至25℃的溫度。反應(yīng)時間可以為2h-48h，優(yōu)選6h-24h，特別優(yōu)選12h。下面介紹若干實(shí)施例，其不解釋為具有限制性。這些反應(yīng)可以以經(jīng)典的配方在反應(yīng)燒瓶中在攪拌下進(jìn)行。為了能夠以高對映體過量制備(S)-PAC，通過誘變產(chǎn)生酶ApPDC-E469G的變體。變體ApPDC-E469G-I468A-W543F通過使用分離的酶以98%ee生產(chǎn)(S)-PAC。通過使用在大腸桿菌中異源表達(dá)并用作廉價全細(xì)胞催化劑的ApPDC-E469G-I468A-W543F，可以以96%ee生成(S)-PAC。實(shí)施例3：20mM苯甲醛、400mM丙酮酸、2.5mM硫酸鎂、100μM二磷酸硫胺素、20mg/mL（濕重）ApPDC-E469G-I468A-W543F（大腸桿菌的全細(xì)胞，其中ApPDC-E469G-I468A-W543F以表達(dá)形式存在）、50mM磷酸鉀緩沖液pH6.5，25℃，反應(yīng)時間：48h。(S)-PAC的對映體純度：ee96%收率：73%。實(shí)施例4：20mM苯甲醛、400mM丙酮酸、2.5mM硫酸鎂、100μM二磷酸硫胺素、1mg/mLApPDC-E469G-I468A-W543F20mM苯甲醛、400mM丙酮酸、2.5mM硫酸鎂、100μM二磷酸硫胺素、1mg/mLApPDC-E469G-I468G（分離的酶）、50mM磷酸鉀緩沖液pH6.5，25℃，反應(yīng)時間：48h。，50mM磷酸鉀緩沖液pH6.5，25℃，反應(yīng)時間：48h。(S)-PAC的對映體純度：ee98%收率：45%。借助QuikChange?由ApPDC-E469G-I468A-W543起始制備變體ApPDC-E469G-I468A-W543F的定向方法為了制備酶變體ApPDC-E469G-I468A-W543F，使用所謂的QuikChange?-PCR法（U.S.專利號5.789.166、5.932.419、6.391.548）。在PCR的這一變體的情況中，使用在待替換的DNA三聯(lián)體密碼的位置上帶有相應(yīng)的序列修飾的引物對。為了制備酶變體ApPDC-E469G-I468A-W543F，使用編碼變體ApPDC-E469G-I468A-W543的基因。這一所謂的DNA模板在載體（例如pET22a）中以克隆形式存在。代替在位置W543中編碼氨基酸色氨酸的三聯(lián)體密碼，必須使用在該位置上帶有編碼苯丙氨酸的突變的引物（即例如TTC或TTT）。用于由ApPDC-E469G-I468A-W543起始制備酶變體ApPDC-E469G-I468A-W543F的QuikChange?-PCR法的引物正向：5′accttgcggccgaattgaaccagcatatccgtgc3′（序列號50）反向：5′gcacggatatgctggttcaattcggccgcaaggt3′（序列號51）。首先制備母液并隨后分為四個各為50μl的配制品。為了開始該反應(yīng)，添加1μl“PfuTurbo?DNA聚合酶”。PCR-反應(yīng)配方：該反應(yīng)在下列條件下進(jìn)行：為了消化模板DNA，將1μl的酶DpnI（Eppendorf）添加到溶液中并在37℃下培養(yǎng)1h。整個配制品然后用“DNA純化試劑盒”（化學(xué)品列表）在進(jìn)一步轉(zhuǎn)化前進(jìn)行提純。這一QuikChange?-PCR法的所有其它參數(shù)以及對于其它酶變體的所需引物的選擇可以類似于制造商（AgilentTechnologies,Inc.）的說明借助“QuikChange?定點(diǎn)誘變試劑盒”的指導(dǎo)來使用。制備變體ApPDC-E469G-I468A-W543F的QuikChange?-PCR法的DNA模板（ApPDC-E469G-I468A）。序列號52在此例如對于編碼根據(jù)序列號2的待修飾的蛋白ApPDC-E469G-I468A的DNA公開。但是，根據(jù)本發(fā)明，編碼待修飾的起始蛋白的所有其它脫氧核糖核酸可以用于制備待修飾的酶。為其編碼的核苷酸是本領(lǐng)域技術(shù)人員已知的。大腸桿菌BL21-DE3和大腸桿菌DH5α的轉(zhuǎn)化菌株大腸桿菌BL21-DE3和大腸桿菌DH5α用通過“位點(diǎn)飽和誘變”制備的DNA進(jìn)行轉(zhuǎn)化。為此，向50μl感受態(tài)細(xì)胞中添加100ngDNA并在冰上培養(yǎng)30分鐘。隨后在42℃下進(jìn)行熱休克90秒。3分鐘后，向冰上添加500μlSOC培養(yǎng)基并然后將該溶液在350轉(zhuǎn)/分鐘下且在37℃下在Eppendorf恒溫混勻儀中培養(yǎng)45分鐘。在完成培養(yǎng)后，將細(xì)胞懸浮液在13000轉(zhuǎn)/分鐘下在Eppendorf臺式離心機(jī)中離心分離30秒，并將沉淀物接著再懸浮在100μl上清液中。濃縮至100μl的細(xì)胞懸浮液在LB瓊脂平板（具有100μg/ml氨芐青霉素）上鋪板并在37℃下倒立著培養(yǎng)16h。DNA分離和通過DNA測序的最佳酶變體的識別將碳連接反應(yīng)中對于(S)-PAC提供最高ee值的酶的DNA由母板起始進(jìn)行測序以識別突變。為此，首先將細(xì)胞用接種環(huán)從加入有甘油的母板轉(zhuǎn)移到50mLLB培養(yǎng)基（+50μg/ml氨芐青霉素）中并在250mL錐形瓶中在37℃下培養(yǎng)。在12h培養(yǎng)后，將20mL細(xì)胞懸浮液離心分離（4000轉(zhuǎn)/分鐘，5min，4℃）。沉淀物中的細(xì)胞的DNA類似于制造商說明（QiagenN.V.）根據(jù)“QIAprep?SpinMiniprepKit”方法進(jìn)行分離。為此，將DNA的濃度設(shè)定為100ng/μl并通過LGCGenomicsGmbH公司測序。LB（溶菌肉湯）培養(yǎng)基“全細(xì)胞”形式的變體的制備為了以1L的規(guī)模在全細(xì)胞中表達(dá)酶，首先將細(xì)胞從加入有甘油的母板用接種環(huán)轉(zhuǎn)移到50mLLB培養(yǎng)基（+100μg/ml氨芐青霉素）中并在250mL錐形瓶中在120轉(zhuǎn)/分鐘和37℃下培養(yǎng)。在16h培養(yǎng)后，向1L自動誘導(dǎo)培養(yǎng)基中加入10mL培養(yǎng)物并在5L錐形瓶中在90轉(zhuǎn)/分鐘和20℃下培養(yǎng)72h。隨后通過離心（4℃，6000轉(zhuǎn)/分鐘，30min）收獲該細(xì)胞并在-20℃下儲存以進(jìn)一步使用。自動誘導(dǎo)培養(yǎng)基12g/l蛋白胨24g/l酵母提取物90mM磷酸鉀緩沖液(pH7.5)0.5g/l葡萄糖2g/l乳糖0.01g/l氨芐青霉素6.3g/l甘油分離的酶的形式的變體的制備將10g以1L規(guī)模培養(yǎng)的細(xì)胞用冷卻到4℃的25mL破碎緩沖液（50mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂）在冰上再懸浮。隨后，將再懸浮的細(xì)胞借助超聲（SD14超聲波發(fā)生器（HielscherUltrasonicsGmbH），4x2min超聲處理，各1分鐘來自冰的冷卻）進(jìn)行破碎。為了分離細(xì)胞碎片，將該溶液離心分離（45min，18000轉(zhuǎn)/分鐘，4℃）并將上清液（“細(xì)胞提取物”）轉(zhuǎn)移到新容器中。為了借助固定化金屬離子親合色譜法和尺寸排阻色譜法提純ApPDC變體，使用“AmershamBioscience”公司的“?KTA?purifier”，以尤其檢測蛋白-UV吸收（280nm）以及導(dǎo)電性并調(diào)節(jié)流速。為了提純，將制備的細(xì)胞提取物（~25mL）以3mL/min的流速施加到體積為60mL的柱Ni-NTA-Superflow（QiagenN.V.）上，其事前用180mL上樣緩沖液進(jìn)行平衡。隨后，繼續(xù)用上樣緩沖液以5ml/min的流速沖洗，以除去不結(jié)合或非常弱地結(jié)合在柱材料上的蛋白。在UV吸收（280nm）重新達(dá)到穩(wěn)定基線后，漂洗緩沖液（50mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂、50mM咪唑）以5mL/min流速用于洗脫弱結(jié)合在柱材料上的蛋白。在重新達(dá)到穩(wěn)定UV吸收（280nm）后，以5mL/min的流速使用洗脫緩沖液（50mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂、250mM咪唑）以洗脫目標(biāo)蛋白。將IMAC的洗出液以10mL/min的流速施加到尺寸排阻色譜柱（1L柱體積,Sephadex-G25,GE-Healthcare）上以進(jìn)行再緩沖，該柱事先用2L再緩沖緩沖液（10mM磷酸鉀pH6.5、100μM二磷酸硫胺素、2mM硫酸鎂）沖洗。合并具有提高的UV吸收（280nm）的級分并在結(jié)晶皿中冷凍（-20℃）。為了凍干，向冷凍的蛋白溶液施加0.22mBar的負(fù)壓3天。所產(chǎn)生的粉末的蛋白含量為20%。純度（目標(biāo)蛋白相對于外源蛋白的含量）為>90%。序列表<110>ForschungszentrumJülichGmbH<120>裂解酶以及不對稱合成苯基乙酰基甲醇的方法<130>PT1.2668<160>53<170>PatentInversion3.5<210>1<211>560<212>PRT<213>人工<220><223>人工制備<400>1MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValGlyGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnTrpGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>2<211>560<212>PRT<213>人工<220><223>人工制備<400>2MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValAlaGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnTrpGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>3<211>560<212>PRT<213>人工<220><223>人工制備<400>3MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValValGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnTrpGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>4<211>1680<212>DNA<213>人工<220><223>人工制備<400>4atgacctatactgttggcatgtatcttgcagaacgccttgtacagatcgggctgaagcat60cacttcgccgtggcgggcgactacaatctcgttcttctggatcagttgctcctcaacaag120gacatgaaacagatctattgctgcaatgagttgaactgtggcttcagcgcggaaggctac180gcccgttctaacggggctgcggcagcggttgtcaccttcagcgttggcgccatttccgcc240atgaacgccctcggcggcgcctatgccgaaaacctgccggttatcctgatttccggcgcg300cccaacagcaatgatcagggcacaggtcatatcctgcatcacacaatcggcaagacggat360tacagctaccagcttgaaatggcccgtcaggtcacctgtgccgccgaaagcattaccgac420gctcactccgccccggccaagattgaccacgtcattcgcacggcgctgcgcgagcgtaag480ccggcctatctggacatcgcgtgcaacattgcctccgagccctgcgtgcggcctggccct540gtcagcagcctgctgtccgagcctgaaatcgaccacacgagcctgaaggccgcagtggac600gccacggttgccttgctggaaaaatcggccagccccgtcatgctgctgggcagcaagctg660cgggccgccaacgcactggccgcaaccgaaacgctggcagacaagctgcaatgcgcggtg720accatcatggcggccgcgaaaggctttttccccgaagaccacgcgggtttccgcggcctg780tactggggcgaagtctcgaaccccggcgtgcaggaactggtggagacctccgacgcactg840ctgtgcatcgcccccgtattcaacgactattcaacagtcggctggtcggcatggcccaag900ggccccaatgtgattctggctgagcccgaccgcgtaacggtcgatggccgcgcctatgac960ggctttaccctgcgcgccttcctgcaggctctggcggaaaaagcccccgcgcgcccggcc1020tccgcacagaaaagcagcgtcccgacgtgctcgctcaccgcgacatccgatgaagccggt1080ctgacgaatgacgaaatcgtccgtcatatcaacgccctgctgacatcaaacacgacgctg1140gtggcagaaaccggcgattcatggttcaatgccatgcgcatgaccctgccgcgcggtgcg1200cgcgtggaactggaaatgcagtggggccatatcggctggtccgtgccctccgccttcggc1260aatgccatgggctcgcaggaccgccagcatgtggtgatggtaggcgatggctccttccag1320cttaccgcgcaggaagtggctcagatggtgcgctacgaactgcccgtcattatctttctg1380atcaacaaccgtggctatgtcggtggcatcgccattcatgacggcccgtacaactatatc1440aagaactgggattacgccggcctgatggaagtcttcaacgccggagaaggccatggactt1500ggcctgaaagccaccaccccgaaggaactgacagaagccatcgccagggcaaaagccaat1560acccgcggcccgacgctgatcgaatgccagatcgaccgcacggactgcacggatatgctg1620gttcaatggggccgcaaggttgcctcaaccaacgcgcgcaagaccactctggccctcgag1680<210>5<211>7041<212>DNA<213>人工<220><223>人工制備<400>5tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcg60cagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttc120ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagg180gttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttc240acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt300ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc360ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta420acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttt480tcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgta540tccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtat600gagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt660ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacg720agtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccga780agaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccg840tattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggt900tgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatg960cagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcgg1020aggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttga1080tcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcc1140tgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttc1200ccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctc1260ggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcg1320cggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacac1380gacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctc1440actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgattt1500aaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgac1560caaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa1620aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacc1680accgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggt1740aactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttagg1800ccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttacc1860agtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtt1920accggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgga1980gcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgct2040tcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcg2100cacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcca2160cctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaa2220cgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtt2280ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga2340taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga2400gcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatgg2460tgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctat2520cgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccct2580gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagct2640gcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagct2700catcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgt2760tgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcgg2820ttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaa2880tgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgccc2940ggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaa3000aaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggta3060gccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcg3120tttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcag3180acgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaac3240cagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgca3300cccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgg3360gaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggc3420cgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccg3480gcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtca3540tgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgag3600atcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctt3660tccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag3720gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagc3780tgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgc3840cccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtct3900tcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggta3960atggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacg4020atgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcct4080tcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccaga4140cgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc4200aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactg4260ttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagct4320tccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgt4380tgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatc4440gacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgc4500gacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgccc4560gccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccact4620ttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctga4680taagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccacc4740ctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcg4800atggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccag4860tagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggc4920gcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcat4980gagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagc5040aaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagat5100ctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcc5160cctctagaaataattttgtttaactttaagaaggagatatacatatgacctatactgttg5220gcatgtatcttgcagaacgccttgtacagatcgggctgaagcatcacttcgccgtggcgg5280gcgactacaatctcgttcttctggatcagttgctcctcaacaaggacatgaaacagatct5340attgctgcaatgagttgaactgtggcttcagcgcggaaggctacgcccgttctaacgggg5400ctgcggcagcggttgtcaccttcagcgttggcgccatttccgccatgaacgccctcggcg5460gcgcctatgccgaaaacctgccggttatcctgatttccggcgcgcccaacagcaatgatc5520agggcacaggtcatatcctgcatcacacaatcggcaagacggattacagctaccagcttg5580aaatggcccgtcaggtcacctgtgccgccgaaagcattaccgacgctcactccgccccgg5640ccaagattgaccacgtcattcgcacggcgctgcgcgagcgtaagccggcctatctggaca5700tcgcgtgcaacattgcctccgagccctgcgtgcggcctggccctgtcagcagcctgctgt5760ccgagcctgaaatcgaccacacgagcctgaaggccgcagtggacgccacggttgccttgc5820tggaaaaatcggccagccccgtcatgctgctgggcagcaagctgcgggccgccaacgcac5880tggccgcaaccgaaacgctggcagacaagctgcaatgcgcggtgaccatcatggcggccg5940cgaaaggctttttccccgaagaccacgcgggtttccgcggcctgtactggggcgaagtct6000cgaaccccggcgtgcaggaactggtggagacctccgacgcactgctgtgcatcgcccccg6060tattcaacgactattcaacagtcggctggtcggcatggcccaagggccccaatgtgattc6120tggctgagcccgaccgcgtaacggtcgatggccgcgcctatgacggctttaccctgcgcg6180ccttcctgcaggctctggcggaaaaagcccccgcgcgcccggcctccgcacagaaaagca6240gcgtcccgacgtgctcgctcaccgcgacatccgatgaagccggtctgacgaatgacgaaa6300tcgtccgtcatatcaacgccctgctgacatcaaacacgacgctggtggcagaaaccggcg6360attcatggttcaatgccatgcgcatgaccctgccgcgcggtgcgcgcgtggaactggaaa6420tgcagtggggccatatcggctggtccgtgccctccgccttcggcaatgccatgggctcgc6480aggaccgccagcatgtggtgatggtaggcgatggctccttccagcttaccgcgcaggaag6540tggctcagatggtgcgctacgaactgcccgtcattatctttctgatcaacaaccgtggct6600atgtcggtggcatcgccattcatgacggcccgtacaactatatcaagaactgggattacg6660ccggcctgatggaagtcttcaacgccggagaaggccatggacttggcctgaaagccacca6720ccccgaaggaactgacagaagccatcgccagggcaaaagccaatacccgcggcccgacgc6780tgatcgaatgccagatcgaccgcacggactgcacggatatgctggttcaatggggccgca6840aggttgcctcaaccaacgcgcgcaagaccactctggccctcgagcaccaccaccaccacc6900actgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctg6960agcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctga7020aaggaggaactatatccggat7041<210>6<211>29<212>DNA<213>人工<220><223>人工制備<220><221>misc_feature<222>(14)..(14)<223>n是a、c、g或t<400>6ccgtggctatgtcndtggcatcgccattc29<210>7<211>29<212>DNA<213>人工<220><223>人工制備<220><221>misc_feature<222>(16)..(16)<223>n是a、c、g或t<400>7gaatggcgatgccahngacatagccacgg29<210>8<211>1680<212>DNA<213>人工<220><223>人工制備<400>8atgacctatactgttggcatgtatcttgcagaacgccttgtacagatcgggctgaagcat60cacttcgccgtggcgggcgactacaatctcgttcttctggatcagttgctcctcaacaag120gacatgaaacagatctattgctgcaatgagttgaactgtggcttcagcgcggaaggctac180gcccgttctaacggggctgcggcagcggttgtcaccttcagcgttggcgccatttccgcc240atgaacgccctcggcggcgcctatgccgaaaacctgccggttatcctgatttccggcgcg300cccaacagcaatgatcagggcacaggtcatatcctgcatcacacaatcggcaagacggat360tacagctaccagcttgaaatggcccgtcaggtcacctgtgccgccgaaagcattaccgac420gctcactccgccccggccaagattgaccacgtcattcgcacggcgctgcgcgagcgtaag480ccggcctatctggacatcgcgtgcaacattgcctccgagccctgcgtgcggcctggccct540gtcagcagcctgctgtccgagcctgaaatcgaccacacgagcctgaaggccgcagtggac600gccacggttgccttgctggaaaaatcggccagccccgtcatgctgctgggcagcaagctg660cgggccgccaacgcactggccgcaaccgaaacgctggcagacaagctgcaatgcgcggtg720accatcatggcggccgcgaaaggctttttccccgaagaccacgcgggtttccgcggcctg780tactggggcgaagtctcgaaccccggcgtgcaggaactggtggagacctccgacgcactg840ctgtgcatcgcccccgtattcaacgactattcaacagtcggctggtcggcatggcccaag900ggccccaatgtgattctggctgagcccgaccgcgtaacggtcgatggccgcgcctatgac960ggctttaccctgcgcgccttcctgcaggctctggcggaaaaagcccccgcgcgcccggcc1020tccgcacagaaaagcagcgtcccgacgtgctcgctcaccgcgacatccgatgaagccggt1080ctgacgaatgacgaaatcgtccgtcatatcaacgccctgctgacatcaaacacgacgctg1140gtggcagaaaccggcgattcatggttcaatgccatgcgcatgaccctgccgcgcggtgcg1200cgcgtggaactggaaatgcagtggggccatatcggctggtccgtgccctccgccttcggc1260aatgccatgggctcgcaggaccgccagcatgtggtgatggtaggcgatggctccttccag1320cttaccgcgcaggaagtggctcagatggtgcgctacgaactgcccgtcattatctttctg1380atcaacaaccgtggctatgtcattggcatcgccattcatgacggcccgtacaactatatc1440aagaactgggattacgccggcctgatggaagtcttcaacgccggagaaggccatggactt1500ggcctgaaagccaccaccccgaaggaactgacagaagccatcgccagggcaaaagccaat1560acccgcggcccgacgctgatcgaatgccagatcgaccgcacggactgcacggatatgctg1620gttcaatggggccgcaaggttgcctcaaccaacgcgcgcaagaccactctggccctcgag1680<210>9<211>560<212>PRT<213>人工<220><223>人工制備<400>9MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValGlyGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnHisGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>10<211>560<212>PRT<213>人工<220><223>人工制備<400>10MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValGlyGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnPheGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>11<211>560<212>PRT<213>人工<220><223>人工制備<400>11MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValGlyGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnProGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>12<211>560<212>PRT<213>人工<220><223>人工制備<400>12MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValGlyGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnIleGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>13<211>560<212>PRT<213>人工<220><223>人工制備<400>13MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValGlyGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnLeuGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>14<211>560<212>PRT<213>人工<220><223>人工制備<400>14MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValGlyGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnMetGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>15<211>560<212>PRT<213>人工<220><223>人工制備<400>15MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln4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IleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValValGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnGlyGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>46<211>560<212>PRT<213>人工<220><223>人工制備<400>46MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValValGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnSerGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>47<211>560<212>PRT<213>人工<220><223>人工制備<400>47MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValValGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnCysGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560<210>48<211>1680<212>DNA<213>人工<220><223>人工制備<400>48atgacctatactgttggcatgtatcttgcagaacgccttgtacagatcgggctgaagcat60cacttcgccgtggcgggcgactacaatctcgttcttctggatcagttgctcctcaacaag120gacatgaaacagatctattgctgcaatgagttgaactgtggcttcagcgcggaaggctac180gcccgttctaacggggctgcggcagcggttgtcaccttcagcgttggcgccatttccgcc240atgaacgccctcggcggcgcctatgccgaaaacctgccggttatcctgatttccggcgcg300cccaacagcaatgatcagggcacaggtcatatcctgcatcacacaatcggcaagacggat360tacagctaccagcttgaaatggcccgtcaggtcacctgtgccgccgaaagcattaccgac420gctcactccgccccggccaagattgaccacgtcattcgcacggcgctgcgcgagcgtaag480ccggcctatctggacatcgcgtgcaacattgcctccgagccctgcgtgcggcctggccct540gtcagcagcctgctgtccgagcctgaaatcgaccacacgagcctgaaggccgcagtggac600gccacggttgccttgctggaaaaatcggccagccccgtcatgctgctgggcagcaagctg660cgggccgccaacgcactggccgcaaccgaaacgctggcagacaagctgcaatgcgcggtg720accatcatggcggccgcgaaaggctttttccccgaagaccacgcgggtttccgcggcctg780tactggggcgaagtctcgaaccccggcgtgcaggaactggtggagacctccgacgcactg840ctgtgcatcgcccccgtattcaacgactattcaacagtcggctggtcggcatggcccaag900ggccccaatgtgattctggctgagcccgaccgcgtaacggtcgatggccgcgcctatgac960ggctttaccctgcgcgccttcctgcaggctctggcggaaaaagcccccgcgcgcccggcc1020tccgcacagaaaagcagcgtcccgacgtgctcgctcaccgcgacatccgatgaagccggt1080ctgacgaatgacgaaatcgtccgtcatatcaacgccctgctgacatcaaacacgacgctg1140gtggcagaaaccggcgattcatggttcaatgccatgcgcatgaccctgccgcgcggtgcg1200cgcgtggaactggaaatgcagtggggccatatcggctggtccgtgccctccgccttcggc1260aatgccatgggctcgcaggaccgccagcatgtggtgatggtaggcgatggctccttccag1320cttaccgcgcaggaagtggctcagatggtgcgctacgaactgcccgtcattatctttctg1380atcaacaaccgtggctatgtcgccggcatcgccattcatgacggcccgtacaactatatc1440aagaactgggattacgccggcctgatggaagtcttcaacgccggagaaggccatggactt1500ggcctgaaagccaccaccccgaaggaactgacagaagccatcgccagggcaaaagccaat1560acccgcggcccgacgctgatcgaatgccagatcgaccgcacggactgcacggatatgctg1620gttcaatttggccgcaaggttgcctcaaccaacgcgcgcaagaccactctggccctcgag1680<210>49<211>7041<212>DNA<213>人工<220><223>人工制備<400>49tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcg60cagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttc120ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagg180gttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttc240acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt300ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc360ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta420acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttt480tcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgta540tccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtat600gagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt660ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacg720agtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccga780agaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccg840tattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggt900tgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatg960cagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcgg1020aggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttga1080tcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcc1140tgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttc1200ccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctc1260ggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcg1320cggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacac1380gacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctc1440actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgattt1500aaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgac1560caaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa1620aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacc1680accgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggt1740aactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttagg1800ccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttacc1860agtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtt1920accggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgga1980gcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgct2040tcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcg2100cacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcca2160cctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaa2220cgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtt2280ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga2340taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga2400gcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatgg2460tgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctat2520cgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccct2580gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagct2640gcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagct2700catcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgt2760tgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcgg2820ttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaa2880tgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgccc2940ggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaa3000aaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggta3060gccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcg3120tttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcag3180acgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaac3240cagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgca3300cccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgg3360gaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggc3420cgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccg3480gcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtca3540tgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgag3600atcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctt3660tccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag3720gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagc3780tgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgc3840cccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtct3900tcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggta3960atggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacg4020atgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcct4080tcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccaga4140cgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc4200aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactg4260ttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagct4320tccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgt4380tgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatc4440gacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgc4500gacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgccc4560gccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccact4620ttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctga4680taagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccacc4740ctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcg4800atggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccag4860tagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggc4920gcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcat4980gagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagc5040aaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagat5100ctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcc5160cctctagaaataattttgtttaactttaagaaggagatatacatatgacctatactgttg5220gcatgtatcttgcagaacgccttgtacagatcgggctgaagcatcacttcgccgtggcgg5280gcgactacaatctcgttcttctggatcagttgctcctcaacaaggacatgaaacagatct5340attgctgcaatgagttgaactgtggcttcagcgcggaaggctacgcccgttctaacgggg5400ctgcggcagcggttgtcaccttcagcgttggcgccatttccgccatgaacgccctcggcg5460gcgcctatgccgaaaacctgccggttatcctgatttccggcgcgcccaacagcaatgatc5520agggcacaggtcatatcctgcatcacacaatcggcaagacggattacagctaccagcttg5580aaatggcccgtcaggtcacctgtgccgccgaaagcattaccgacgctcactccgccccgg5640ccaagattgaccacgtcattcgcacggcgctgcgcgagcgtaagccggcctatctggaca5700tcgcgtgcaacattgcctccgagccctgcgtgcggcctggccctgtcagcagcctgctgt5760ccgagcctgaaatcgaccacacgagcctgaaggccgcagtggacgccacggttgccttgc5820tggaaaaatcggccagccccgtcatgctgctgggcagcaagctgcgggccgccaacgcac5880tggccgcaaccgaaacgctggcagacaagctgcaatgcgcggtgaccatcatggcggccg5940cgaaaggctttttccccgaagaccacgcgggtttccgcggcctgtactggggcgaagtct6000cgaaccccggcgtgcaggaactggtggagacctccgacgcactgctgtgcatcgcccccg6060tattcaacgactattcaacagtcggctggtcggcatggcccaagggccccaatgtgattc6120tggctgagcccgaccgcgtaacggtcgatggccgcgcctatgacggctttaccctgcgcg6180ccttcctgcaggctctggcggaaaaagcccccgcgcgcccggcctccgcacagaaaagca6240gcgtcccgacgtgctcgctcaccgcgacatccgatgaagccggtctgacgaatgacgaaa6300tcgtccgtcatatcaacgccctgctgacatcaaacacgacgctggtggcagaaaccggcg6360attcatggttcaatgccatgcgcatgaccctgccgcgcggtgcgcgcgtggaactggaaa6420tgcagtggggccatatcggctggtccgtgccctccgccttcggcaatgccatgggctcgc6480aggaccgccagcatgtggtgatggtaggcgatggctccttccagcttaccgcgcaggaag6540tggctcagatggtgcgctacgaactgcccgtcattatctttctgatcaacaaccgtggct6600atgtcgccggcatcgccattcatgacggcccgtacaactatatcaagaactgggattacg6660ccggcctgatggaagtcttcaacgccggagaaggccatggacttggcctgaaagccacca6720ccccgaaggaactgacagaagccatcgccagggcaaaagccaatacccgcggcccgacgc6780tgatcgaatgccagatcgaccgcacggactgcacggatatgctggttcaatttggccgca6840aggttgcctcaaccaacgcgcgcaagaccactctggccctcgagcaccaccaccaccacc6900actgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctg6960agcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctga7020aaggaggaactatatccggat7041<210>50<211>34<212>DNA<213>人工<220><223>人工制備<400>50accttgcggccgaattgaaccagcatatccgtgc34<210>51<211>34<212>DNA<213>人工<220><223>人工制備<400>51gcacggatatgctggttcaattcggccgcaaggt34<210>52<211>1680<212>DNA<213>人工<220><223>人工制備<400>52atgacctatactgttggcatgtatcttgcagaacgccttgtacagatcgggctgaagcat60cacttcgccgtggcgggcgactacaatctcgttcttctggatcagttgctcctcaacaag120gacatgaaacagatctattgctgcaatgagttgaactgtggcttcagcgcggaaggctac180gcccgttctaacggggctgcggcagcggttgtcaccttcagcgttggcgccatttccgcc240atgaacgccctcggcggcgcctatgccgaaaacctgccggttatcctgatttccggcgcg300cccaacagcaatgatcagggcacaggtcatatcctgcatcacacaatcggcaagacggat360tacagctaccagcttgaaatggcccgtcaggtcacctgtgccgccgaaagcattaccgac420gctcactccgccccggccaagattgaccacgtcattcgcacggcgctgcgcgagcgtaag480ccggcctatctggacatcgcgtgcaacattgcctccgagccctgcgtgcggcctggccct540gtcagcagcctgctgtccgagcctgaaatcgaccacacgagcctgaaggccgcagtggac600gccacggttgccttgctggaaaaatcggccagccccgtcatgctgctgggcagcaagctg660cgggccgccaacgcactggccgcaaccgaaacgctggcagacaagctgcaatgcgcggtg720accatcatggcggccgcgaaaggctttttccccgaagaccacgcgggtttccgcggcctg780tactggggcgaagtctcgaaccccggcgtgcaggaactggtggagacctccgacgcactg840ctgtgcatcgcccccgtattcaacgactattcaacagtcggctggtcggcatggcccaag900ggccccaatgtgattctggctgagcccgaccgcgtaacggtcgatggccgcgcctatgac960ggctttaccctgcgcgccttcctgcaggctctggcggaaaaagcccccgcgcgcccggcc1020tccgcacagaaaagcagcgtcccgacgtgctcgctcaccgcgacatccgatgaagccggt1080ctgacgaatgacgaaatcgtccgtcatatcaacgccctgctgacatcaaacacgacgctg1140gtggcagaaaccggcgattcatggttcaatgccatgcgcatgaccctgccgcgcggtgcg1200cgcgtggaactggaaatgcagtggggccatatcggctggtccgtgccctccgccttcggc1260aatgccatgggctcgcaggaccgccagcatgtggtgatggtaggcgatggctccttccag1320cttaccgcgcaggaagtggctcagatggtgcgctacgaactgcccgtcattatctttctg1380atcaacaaccgtggctatgtcgccggcatcgccattcatgacggcccgtacaactatatc1440aagaactgggattacgccggcctgatggaagtcttcaacgccggagaaggccatggactt1500ggcctgaaagccaccaccccgaaggaactgacagaagccatcgccagggcaaaagccaat1560acccgcggcccgacgctgatcgaatgccagatcgaccgcacggactgcacggatatgctg1620gttcaatggggccgcaaggttgcctcaaccaacgcgcgcaagaccactctggccctcgag1680<210>53<211>560<212>PRT<213>人工<220><223>人工制備<400>53MetThrTyrThrValGlyMetTyrLeuAlaGluArgLeuValGlnIle151015GlyLeuLysHisHisPheAlaValAlaGlyAspTyrAsnLeuValLeu202530LeuAspGlnLeuLeuLeuAsnLysAspMetLysGlnIleTyrCysCys354045AsnGluLeuAsnCysGlyPheSerAlaGluGlyTyrAlaArgSerAsn505560GlyAlaAlaAlaAlaValValThrPheSerValGlyAlaIleSerAla65707580MetAsnAlaLeuGlyGlyAlaTyrAlaGluAsnLeuProValIleLeu859095IleSerGlyAlaProAsnSerAsnAspGlnGlyThrGlyHisIleLeu100105110HisHisThrIleGlyLysThrAspTyrSerTyrGlnLeuGluMetAla115120125ArgGlnValThrCysAlaAlaGluSerIleThrAspAlaHisSerAla130135140ProAlaLysIleAspHisValIleArgThrAlaLeuArgGluArgLys145150155160ProAlaTyrLeuAspIleAlaCysAsnIleAlaSerGluProCysVal165170175ArgProGlyProValSerSerLeuLeuSerGluProGluIleAspHis180185190ThrSerLeuLysAlaAlaValAspAlaThrValAlaLeuLeuGluLys195200205SerAlaSerProValMetLeuLeuGlySerLysLeuArgAlaAlaAsn210215220AlaLeuAlaAlaThrGluThrLeuAlaAspLysLeuGlnCysAlaVal225230235240ThrIleMetAlaAlaAlaLysGlyPhePheProGluAspHisAlaGly245250255PheArgGlyLeuTyrTrpGlyGluValSerAsnProGlyValGlnGlu260265270LeuValGluThrSerAspAlaLeuLeuCysIleAlaProValPheAsn275280285AspTyrSerThrValGlyTrpSerAlaTrpProLysGlyProAsnVal290295300IleLeuAlaGluProAspArgValThrValAspGlyArgAlaTyrAsp305310315320GlyPheThrLeuArgAlaPheLeuGlnAlaLeuAlaGluLysAlaPro325330335AlaArgProAlaSerAlaGlnLysSerSerValProThrCysSerLeu340345350ThrAlaThrSerAspGluAlaGlyLeuThrAsnAspGluIleValArg355360365HisIleAsnAlaLeuLeuThrSerAsnThrThrLeuValAlaGluThr370375380GlyAspSerTrpPheAsnAlaMetArgMetThrLeuProArgGlyAla385390395400ArgValGluLeuGluMetGlnTrpGlyHisIleGlyTrpSerValPro405410415SerAlaPheGlyAsnAlaMetGlySerGlnAspArgGlnHisValVal420425430MetValGlyAspGlySerPheGlnLeuThrAlaGlnGluValAlaGln435440445MetValArgTyrGluLeuProValIleIlePheLeuIleAsnAsnArg450455460GlyTyrValIleGlyIleAlaIleHisAspGlyProTyrAsnTyrIle465470475480LysAsnTrpAspTyrAlaGlyLeuMetGluValPheAsnAlaGlyGlu485490495GlyHisGlyLeuGlyLeuLysAlaThrThrProLysGluLeuThrGlu500505510AlaIleAlaArgAlaLysAlaAsnThrArgGlyProThrLeuIleGlu515520525CysGlnIleAspArgThrAspCysThrAspMetLeuValGlnTrpGly530535540ArgLysValAlaSerThrAsnAlaArgLysThrThrLeuAlaLeuGlu545550555560當(dāng)前第1頁1 2 3