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一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法葡萄糖檢測試劑的制作方法

文檔序號:12697570閱讀:來源:國知局

技術(shù)特征:

1.1)一種穩(wěn)定性強(qiáng)的且成本低的己糖激酶法葡萄糖(GLU)檢測試劑組成成分如下:

試劑1(R1)的組成成分:

PH=7.4, TRIS(三羥甲基氨基甲烷)緩沖液··················100mmol/L

Mg2+··················································2mmol/L-4 mmol/L

離子平衡劑············································2g/L-5g/L

疊氮鈉················································0.1g/L-1g/L

NAD(氧化型性輔酶I)····································0.5mmol/L-1 mmol/L

保護(hù)劑················································5g/L-10g/L

表面活性劑1···········································1ml/L-5ml/L

表面活性劑2···········································1ml/L-5ml/L

試劑2(R2)的組成成分:

緩沖液················································10mmol/L

保護(hù)劑1···············································10g/L-20 g/L

保護(hù)劑2···············································2ml-5ml/L

保護(hù)劑3············································..·1mg/L-3mg/L

表面活性劑············································1ml/L-3ml/L

防腐劑················································0.1g/L-1g/L

己糖激酶(HK)··········································100U/L

葡萄糖-6-磷酸脫氫酶(G6P-DH)···························80U/L

ATP.NA2···············································4mmol/L。

2.根據(jù)權(quán)利要求1所述的一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法血液葡萄糖(GLU)檢測試劑,其特征在于試劑R2中緩沖液PH=8.6的 甘氨酸-NaOH緩沖液。

3.根據(jù)權(quán)利要求1所述的一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法血液葡萄糖(GLU)檢測試劑,其特征在于試劑2中所述防腐劑為MIT(2-甲基-4-異噻唑啉-3酮鹽酸鹽)。

4.根據(jù)權(quán)利要求1所述的一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法血液葡萄糖(GLU)檢測試劑,其特征在于試劑R1中所述離子平衡劑為硫酸鈉。

5.根據(jù)權(quán)利要求1所述的一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法血液葡萄糖(GLU)檢測試劑,其特征在于試劑R1所述保護(hù)劑為海藻糖。

6.根據(jù)權(quán)利要求1所述的一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法血液葡萄糖(GLU)檢測試劑,其特征在于試劑R1中所述的表面活性劑1為Pluorinic F68(上海西寶),表面活性劑2為Tetronic 1307(上海西寶)。

7.根據(jù)權(quán)利要求1所述的一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法血液葡萄糖(GLU)檢測試劑,其特征在于試劑R2中所述的保護(hù)劑1聚乙二醇2萬,保護(hù)劑2為吐溫-20,保護(hù)劑3為FAD。

8.根據(jù)權(quán)利要求1-7中任一項所述的一種穩(wěn)定性強(qiáng)且成本低的己糖激酶法血液葡萄糖(GLU)檢測試劑,來檢測血液GLU,,其特征在于使用全自動生化分析儀利用終點法進(jìn)行測定,檢測主波長為340,試劑為液態(tài)雙試劑,試劑比例為4:1。

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