1.一種血清腺苷脫氨酶檢測(cè)試劑,其特征在于包括試劑R1和試劑R2,所述試劑R1和試劑R2的組成如下:
1)試劑R1的組分為:
緩沖液·························100mmol/L,
4-氨基氨替吡啉·················5mmol/L,
PNP(嘌呤核苷磷酸化酶)··········1.5KU/L,
XOD(黃嘌呤氧化酶)··············3.1KU/L,
POD(過(guò)氧化物酶)················5KU/L,
膽紅素氧化酶···················3KU/L,
抗壞血酸氧化酶·················3.5KU/L,
丙三醇·························4ml/L,
聚乙二醇6000···················5g/L,
乙二醇·························6ml/L,
甘露醇·························25g/L,
海藻糖·························15g/L,
BSA····························1g/L,
烷基糖苷(APG)··················1g/L,
防腐劑·························0.5g/L;
2)試劑R2的組分為:
緩沖液·························100mmol/L,
腺苷···························14mmol/L,
HTBA(3-羥基-2,4,6-三碘苯甲酸)··5mmol/L,
丙三醇·························4ml/L,
聚乙二醇6000···················5g/L,
乙二醇·························6ml/L,
甘露醇·························25g/L,
海藻糖·························15g/L,
BSA····························1g/L,
烷基糖苷(APG)··················1g/L,
防腐劑·························0.5g/L。
2.根據(jù)權(quán)利要求1所述的血清腺苷脫氨酶檢測(cè)試劑,其特征在于試劑R1中緩沖液為25℃,pH為8.0的HEPES緩沖液。
3.根據(jù)權(quán)利要求1所述的血清腺苷脫氨酶檢測(cè)試劑,其特征在于試劑R2中緩沖液為25℃,pH為4.0的Tris緩沖液。
4.根據(jù)權(quán)利要求1所述的血清腺苷脫氨酶檢測(cè)試劑,其特征在于試劑R1和R2中表面活性劑為烷基糖苷(APG)。
5.根據(jù)權(quán)利要求1所述的血清腺苷脫氨酶檢測(cè)試劑,其特征在于所述防腐劑為NaN3。
6.一種使用權(quán)利要求1-5中任一項(xiàng)所述的血清腺苷脫氨酶檢測(cè)試劑來(lái)檢測(cè)血清腺苷脫氨酶的檢測(cè)方法,其特征在于使用全自動(dòng)生化分析儀利用速率法進(jìn)行測(cè)定,檢測(cè)主波長(zhǎng)為550nm。
7.根據(jù)權(quán)利要求6所述的檢測(cè)方法,其特征在于R1試劑和R2試劑的比例為2:1。