專利名稱：：Fr901464的生物合成基因簇的制作方法
技術(shù)領(lǐng)域：
：本發(fā)明屬于微生物基因資源和基因工程領(lǐng)域，具體涉及抗腫瘤天然產(chǎn)物FR901464的生物合成基因簇的克隆、分析、功能研究及其應(yīng)用。
背景技術(shù)：
：1996年，日本科學(xué)家從假單胞菌(Pseudomonassp.No.2663,FERMBP-3421)的發(fā)酵培養(yǎng)液中分離得到了三種化學(xué)結(jié)構(gòu)非常類似的化合物FR901463，F(xiàn)R901464,FR901465(圖1)，這類化合物通過(guò)一種新發(fā)展的體外篩選系統(tǒng)-轉(zhuǎn)錄調(diào)控得到。它們可以通過(guò)增強(qiáng)SV40啟動(dòng)子的轉(zhuǎn)錄活性[J.Antibiot.(1996)49，1196-1203]，同時(shí)引起細(xì)胞周期停留在Gl和G2，M期[J.Antibiot.(1996)49，1204-1211]，降低致癌基因和腫瘤抑制子基因的mRNA水平降低，從而有效地抑制哺乳動(dòng)物和人類體外腫瘤細(xì)胞的生長(zhǎng)，其中以化合物FR901464的生物活性最好，半致死量(IC5tl)僅為0.6-3.4nM。用FR901464處理的細(xì)胞表型發(fā)生了變化，其作用效果類似于組蛋白去乙酰酶抑制劑(trichostainA)[Cell(1984)38，317-331]，但是在分析分離的組蛋白乙酰化的過(guò)程中，發(fā)現(xiàn)FR901464并不是組蛋白去乙?；傅囊种苿?。FR901464這些意外的藥理學(xué)特性引起了許多化學(xué)家合生物學(xué)家的興趣。實(shí)驗(yàn)證明它們可以延長(zhǎng)腹部生長(zhǎng)腫瘤的小鼠的生命。用FR901464處理細(xì)胞可能破壞染色質(zhì)的結(jié)構(gòu)，同時(shí)產(chǎn)生“生物活性染色質(zhì)”，這一活性物質(zhì)可以導(dǎo)致含有病毒啟動(dòng)子的轉(zhuǎn)錄活性，但是卻抑制內(nèi)源誘導(dǎo)基因，核仁小體DNA片段被內(nèi)源基因消化，細(xì)胞周期阻滯在檢驗(yàn)點(diǎn)。很可能剪切體抑制劑會(huì)在核內(nèi)斑塊引起明顯的形態(tài)學(xué)變化，并在這些斑塊中有部分聚RNA的積累。最近的研究證實(shí)，F(xiàn)R901464和它甲基化的類似物spliceostainA均可以抑制mRNA前體的剪切，通過(guò)非共價(jià)結(jié)合U2小細(xì)胞核核糖核酸蛋白的SF3b亞復(fù)合體[NatureChem.Biol.(2007)3,576-583]。從物理化學(xué)的數(shù)據(jù)可以看出，F(xiàn)R901464及其類似物擁有獨(dú)特的化學(xué)結(jié)構(gòu)，共軛的雙烯及共軛的氨基羰基作為邊鏈。最近，許多小組完成了它的全合成，及其穩(wěn)定性更好的類似物meayamycin-缺少半縮酮基團(tuán)[J.Am.Chem.Soc.(2007)129，2648-2659]，表明比天然產(chǎn)物的活性更高。甲基化修飾FR901464-spliceostatinA，是SF3b剪切體因子的小分子抑制劑。以前的構(gòu)效關(guān)系研究表明FR901464右臂的卟啉環(huán)對(duì)于生物活性很重要，后來(lái)發(fā)現(xiàn)將第四位的碳乙?；瘯?huì)極大的降低化合物的活性。特別的是，在環(huán)氧的位置上引入環(huán)丙烷，F(xiàn)R901464的活性會(huì)完全喪失，因此，環(huán)氧才是最重要的活性位點(diǎn)。環(huán)氧基團(tuán)表明有高度的反應(yīng)活性，在相關(guān)的物理?xiàng)l件下，通過(guò)β消除可以得到兩個(gè)酮烯，其中一個(gè)通過(guò)脫水可以形成呋喃。本發(fā)明以微生物來(lái)源的FR901464為目標(biāo)分子，從克隆生物合成基因簇出發(fā)，采用微生物學(xué)、分子生物學(xué)、生物化學(xué)及有機(jī)化學(xué)相結(jié)合的方法研究其生物合成，通過(guò)對(duì)其生物合成機(jī)制的研究揭示FR901464及其類似物的獨(dú)特化學(xué)結(jié)構(gòu)形成的酶學(xué)機(jī)理，在此基礎(chǔ)上運(yùn)用代謝工程的原理，合理修飾FR901464的生物合成途徑，探索結(jié)構(gòu)穩(wěn)定、活性更好、并能通過(guò)微生物發(fā)酵大量生產(chǎn)的新型藥物。
發(fā)明內(nèi)容本發(fā)明涉及一種具有獨(dú)特聚酮結(jié)構(gòu)的天然產(chǎn)物FR901464的生物合成基因簇的克隆、分析、功能及其應(yīng)用。本發(fā)明中整個(gè)FR901464的生物合成基因簇共20個(gè)基因的核苷酸序列或互補(bǔ)序列(序列1)，其中7個(gè)(fr9C，fr9D，fr9E，fr9F，fr9G，fr9H，fr9I)用于編碼I型聚酮合成酶(PKS)及非核糖體聚肽合成酶(NRPS)，共包含9個(gè)模塊，53個(gè)功能域，負(fù)責(zé)催化FR901464骨架部分聚酮鏈的生物合成；3個(gè)基因(fr9B，fr9J，fr90)編碼的蛋白負(fù)責(zé)將前體丙二酰輔酶A承載到PKS的載體蛋白結(jié)構(gòu)域上；2個(gè)調(diào)控基因(fr9A和fr9Q)；8個(gè)基因(fr9K，fr9M,fr9N,fr9L,fr9P,fr9R,fr9T,fr9S)編碼的蛋白對(duì)FR901464骨架進(jìn)行后修飾。本發(fā)明還提供了一個(gè)編碼轉(zhuǎn)錄調(diào)控子的核苷酸序列，由序列2中的氨基酸序列組成，命名為Fr9A，其基因的核苷酸序列位于序列1中第11932-12816堿基處。本發(fā)明還提供了一個(gè)編碼?；D(zhuǎn)移酶的核苷酸序列，由序列3中的氨基酸序列組成，命名為Fr9B，其基因的核苷酸序列位于序列1中第13131-14213堿基處。本發(fā)明還提供了一個(gè)編碼?；D(zhuǎn)移酶的核苷酸序列，由序列4中的氨基酸序列組成，命名為Fr9J，其基因的核苷酸序列位于序列1中第80014-80904堿基處。本發(fā)明還提供了一個(gè)編碼3-羥基-3-甲基戊二酰(HMG)輔酶A合成酶的核苷酸序列，由序列5中的氨基酸序列組成，命名為Fr9K，其基因的核苷酸序列位于序列1中第80915-82204堿基處。本發(fā)明還提供了一個(gè)編碼烯醇輔酶A水合酶的核苷酸序列，由序列6中的氨基酸序列組成，命名為Fr9L，其基因的核苷酸序列位于序列1中第82222-82977堿基處。本發(fā)明還提供了一個(gè)編碼?；d體蛋白的核苷酸序列，由序列7中的氨基酸序列組成，命名為Fr9M，其基因的核苷酸序列位于序列1中第83053-83292堿基處。本發(fā)明還提供了一個(gè)編碼酮基合成酶的核苷酸序列，由序列8中的氨基酸序列組成，命名為Fr9N，其基因的核苷酸序列位于序列1中第83285-84550堿基處。本發(fā)明還提供了一個(gè)編碼?；D(zhuǎn)移酶的核苷酸序列，由序列9中的氨基酸序列組成，命名為Fr90，其基因的核苷酸序列位于序列1中第84502-85695堿基處。本發(fā)明還提供了一個(gè)氧化酶的核苷酸序列，由序列10中的氨基酸序列組成，命名為Fr9P，其基因的核苷酸序列位于序列1中第86029-87054堿基處。本發(fā)明還提供了一個(gè)細(xì)胞色素P450氧化酶的核苷酸序列，由序列11中的氨基酸序列組成，命名為Fr9R，其基因的核苷酸序列位于序列1中第87831-89279堿基處。本發(fā)明還提供了一個(gè)乙酰基轉(zhuǎn)移酶的核苷酸序列，由序列12中的氨基酸序列組成，命名為Fr9S，其基因的核苷酸序列位于序列1中第89772-90230堿基處。本發(fā)明還提供了一個(gè)二鳥(niǎo)苷酸環(huán)化酶的核苷酸序列，由序列13中的氨基酸序列組成，命名為Fr9T，其基因的核苷酸序列位于序列1中第91112-92515堿基處。本發(fā)明還提供了一個(gè)包括DH*-KR*-GT*-ACP-KS-KR-ACP-KS-ACP-ACP-TE-KS-ACP-C聚酮合成酶結(jié)構(gòu)域的核苷酸序列，由序列14中的氨基酸序列組成，命名為Fr9C，其基因的核苷酸序列位于序列1中第14687-29872堿基處。本發(fā)明還提供了一個(gè)編碼包括C-A-PCP-KS非核糖體聚肽合成酶和I型聚酮合成酶雜和結(jié)構(gòu)域的核苷酸序列，由序列15中的氨基酸序列組成，命名為Fr9D，其基因的核苷酸序列位于序列1中第29945-35257堿基處。本發(fā)明還提供了一個(gè)包括DH-KR-MT聚酮合成酶結(jié)構(gòu)域的核苷酸序列，由序列16中的氨基酸序列組成，命名為fr9E，其基因的核苷酸序列位于序列1中第35257-38823堿基處。本發(fā)明還提供7一個(gè)包括ACP-ER-KS-DH-KR-ACP-KS-DH-KR-MT-ACP-KS聚酮合成酶結(jié)構(gòu)域的核苷酸序列，由序列17中的氨基酸序列組成，命名為Fr9F，其基因的核苷酸序列位于序列1中第38867-53581堿基處。本發(fā)明還提供了一個(gè)包括DH-KR-ACP-KS-KR-ACP聚酮合成酶結(jié)構(gòu)域的核苷酸序列，由序列18中的氨基酸序列組成，命名為Fr9G，其基因的核苷酸序列位于序列1中第53637-62690堿基處。本發(fā)明還提供了一個(gè)包括KS-ECH-ACP-ACP-ACP-KS-OX聚酮合成酶結(jié)構(gòu)域的核苷酸序列，由序列19中的氨基酸序列組成，命名為Fr9H，其基因的核苷酸序列位于序列1中第62806-70794堿基處。本發(fā)明還提供了一個(gè)包括KR-ACP-KS-DH-ACP-KS-TE聚酮合成酶結(jié)構(gòu)域的核苷酸序列，由序列20中的氨基酸序列組成，命名為Fr9I，其基因的核苷酸序列位于序列1中第70798-79954堿基處。本發(fā)明還提供了一個(gè)環(huán)化酶的核苷酸序列，由序列21中的氨基酸序列組成，命名為Fr9Q，其基因的核苷酸序列位于序列1中第87299-87736堿基處。序列1的互補(bǔ)序列可根據(jù)DNA堿基互補(bǔ)原則隨時(shí)得到。序列1的核苷酸序列或部分核苷酸序列可以通過(guò)聚合酶鏈?zhǔn)椒磻?yīng)(PCR)或用合適的限制性內(nèi)切酶酶切相應(yīng)的DNA或使用其他合適的技術(shù)得到。本發(fā)明提供了得到至少包含部分序列1中DNA序列的重組DNA質(zhì)粒的途徑。本發(fā)明還提供了產(chǎn)生FR901464生物合成基因被中斷的微生物體的途徑，至少其中之一的基因包含有序列1中的核苷酸序列。包含本發(fā)明所提供的核苷酸序列或至少部分核苷酸序列的克隆DNA可用于從假單胞菌Pseudomonassp.No.2663基因組文庫(kù)中定位更多的文庫(kù)質(zhì)粒。這些文庫(kù)質(zhì)粒至少包含本發(fā)明中的部分序列。包含本發(fā)明所提供的核苷酸序列或至少部分核苷酸序列可以被修飾或突變。這些途徑包括插入、置換或缺失，聚合酶鏈?zhǔn)椒磻?yīng)，錯(cuò)誤介導(dǎo)聚合酶鏈?zhǔn)椒磻?yīng)，位點(diǎn)特異性突變，不同序列的重新連接，序列的不同部分或與其他來(lái)源的同源序列進(jìn)行定向進(jìn)化(DNAshuffling)，或通過(guò)紫外線或化學(xué)試劑誘變等。包含本發(fā)明所提供的核苷酸序列或至少部分核苷酸序列的克隆基因可以通過(guò)合適的表達(dá)體系在外源宿主中表達(dá)以得到相應(yīng)的酶或其他更高的生物活性或產(chǎn)量。這些外源宿主包括鏈霉菌、假單孢菌、大腸桿菌、芽孢桿菌、酵母、植物和動(dòng)物等。本發(fā)明所提供的氨基酸序列可以用來(lái)分離所需要的蛋白并可用于抗體的制備。包含本發(fā)明所提供的氨基酸序列或至少部分序列的多肽可能在去除或替代某些氨基酸之后仍有生物活性甚至有新的生物學(xué)活性，或者提高了產(chǎn)量或優(yōu)化了蛋白動(dòng)力學(xué)特征或其他致力于得到的性質(zhì)。包含本發(fā)明所提供的核苷酸序列或至少部分核苷酸序列的基因或基因簇可以在異源宿主中表達(dá)并通過(guò)DNA芯片技術(shù)了解它們?cè)谒拗鞔x鏈中的功能。包含本發(fā)明所提供的核苷酸序列或至少部分核苷酸序列的基因或基因簇可以通過(guò)遺傳重組來(lái)構(gòu)建重組質(zhì)粒以獲得新型生物合成途徑，也可以通過(guò)插入、置換、缺失或失活進(jìn)而獲得新型生物合成途徑。包含本發(fā)明所提供的核苷酸序列或至少部分核苷酸序列的克隆基因或DNA片段可以通過(guò)中斷FR901464生物合成的一個(gè)或幾個(gè)步驟而得到新的FR901464結(jié)構(gòu)類似物或前體。包含本發(fā)明所提供的聚酮合成酶可以通過(guò)缺失、插入或失活來(lái)自于相同或不同的聚酮合成酶系統(tǒng)的一個(gè)或多個(gè)聚酮合成酶結(jié)構(gòu)域、模塊或基因而產(chǎn)生新的聚酮化合物。包含本發(fā)明所提供的核苷酸序列或至少部分核苷酸序列的片段或基因可以用來(lái)構(gòu)建聚酮合成酶庫(kù)或聚酮合成酶衍生庫(kù)或組合庫(kù)。本發(fā)明所提供的FR901464骨架的后修飾基因提供了通過(guò)遺傳修飾得到類似物的途徑，所包含的氧化還原反應(yīng)也可有其他應(yīng)用?？傊景l(fā)明所提供的包含F(xiàn)R901464生物合成相關(guān)的所有基因和蛋白信息可以幫助人們理解FR901464家族天然產(chǎn)物的生物合成機(jī)制，為進(jìn)一步遺傳改造提供了材料和知識(shí)。本發(fā)明所提供的基因及其蛋白質(zhì)也可以用來(lái)尋找和發(fā)現(xiàn)可用于醫(yī)藥、工業(yè)或農(nóng)業(yè)的化合物或基因、蛋白。圖1:FR901463，F(xiàn)R901464,FR901465及其類似物的化學(xué)結(jié)構(gòu)圖2:FR901464生物合成基因簇的基因結(jié)構(gòu)和限制性內(nèi)切酶譜。(A)3個(gè)交疊的粘粒代表了假單胞菌菌基因組90kb的DNA區(qū)域，B代表限制性內(nèi)切酶BamHI，實(shí)體表示已被DNA測(cè)序的部分，Probe-Pl，P2和P3代表標(biāo)記的探針部分；(B)FR901464生物合成基因簇的基因組成。圖3:FR901464生物合成途徑推測(cè)。(A)聚酮/聚肽骨架的形成；(B)起始單位；(C)在線環(huán)合形成第一個(gè)六元雜環(huán)；(D)聚酮鏈的β-甲基化修飾；(E)聚酮鏈的解離及后修飾。圖4聚酮、聚肽合成酶的功能分析。(A)起始模塊中甘油?；D(zhuǎn)移酶結(jié)構(gòu)域Gf保守區(qū)域的分析，星號(hào)表示決定功能域活性的關(guān)鍵保守氨基酸；(B)酮基合成酶KS、載體蛋白CP、?；D(zhuǎn)移酶結(jié)構(gòu)域AT、脫水酶結(jié)構(gòu)域DH、縮合酶結(jié)構(gòu)域C、硫酯酶TE及酮基還原酶KR保守區(qū)域的分析；(C)氧化還原酶OX保守區(qū)域的分析。圖5基因置換突變菌株(Afr9K)與野生型發(fā)酵產(chǎn)物的高效液相色譜(HPLC)分析符號(hào)說(shuō)明M模塊；C:縮合酶；A腺苷化酶；PCP肽酰載體蛋白；L起始；NRPS非核糖體肽合成酶；Wavelength波長(zhǎng)；Gf甘油酰基轉(zhuǎn)移酶；KS酮基合成酶；ACP酰基載體蛋白；AT?；D(zhuǎn)移酶；DH脫水酶；TE硫酯酶；KR酮基還原酶；OX氧化酶；MT甲基轉(zhuǎn)移酶；ER烯醇還原酶；ECH烯酰輔酶A水合酶；HMGCS:3_羥基-3-甲基戊二酰輔酶A合成酶。具體實(shí)施例方式以下結(jié)合圖1-圖5對(duì)本發(fā)明進(jìn)一步詳細(xì)說(shuō)明。1.克隆FR901464的生物合成基因片斷盡管科學(xué)家對(duì)FR901464的化學(xué)合成和作用機(jī)制方面的研究很多，但有關(guān)其生物合成起源方面的認(rèn)識(shí)卻非常少。2004年，美國(guó)科學(xué)家William闡述了藍(lán)藻的次級(jí)代謝產(chǎn)物Jamaicamides的生物合成途徑，其中的JamH基因與HMGCSs同源性相當(dāng)高，推測(cè)該酶催化承載在乙?；D(zhuǎn)運(yùn)蛋白上活化的酮基中間體與乙酸單位間的縮合，從而形成承載在乙?；D(zhuǎn)運(yùn)蛋白上的酰基中間體[Chem.Biol.(2004)11，817-833]。這些研究為克隆FR901464的生物合成基因提供了極為重要的信息。本發(fā)明推測(cè)FR901464的生物合成中很可能也包含這一步反應(yīng)，因此克隆策略就是從克隆HMGCS基因入手。本發(fā)明基于已報(bào)告的一些化合物的生物合成序列設(shè)計(jì)了HMGCS通用引物，希望通過(guò)簡(jiǎn)并引物PCR得到相關(guān)的HMGCS基因片段。采用簡(jiǎn)并性引物(5’CTGCCGTAYGARGAYCCNRT3，和5，GTAGAAYTCGCTRCARCANCC3，)PCR克隆編碼HMGCS基因部分序列的方法得到約0.9kb的PCR產(chǎn)物，克隆入pMD18載體，經(jīng)限制性內(nèi)切酶分組，DNA序列分析表明與已知的HMGCS基因有很高的同源性。經(jīng)過(guò)對(duì)FR901464化學(xué)結(jié)構(gòu)的分析，本發(fā)明推測(cè)該化合物應(yīng)該是通過(guò)多個(gè)聚酮合成酶(PKS)合成其骨架。典型的I型重復(fù)性PKS是以模塊形式存在的多功能酶，每一個(gè)模塊含有一套獨(dú)特的，非重復(fù)使用的催化功能域，即催化功能域與產(chǎn)物合成順序是一一對(duì)應(yīng)的。本發(fā)明基于已有的一些PKS途徑來(lái)的化合物的生物合成序列KS功能域設(shè)計(jì)了PKS的通用簡(jiǎn)并性引物(5，GCCTGTTCCAGCTCGCTGRYSRCBMTBCA3，和5，CGCGGTTTCACAATGNCCRAKRTTSSWYTT3，)PCR克隆編碼PKS基因部分序列的方法得到約550bp的PCR產(chǎn)物，克隆入pMD18載體，經(jīng)限制性酶分組，DNA測(cè)序分析表明與已知的PKS基因有很高的同源性。2.FR901464的生物合成基因粗的克隆，順序分析及功能分析將上述HMGCS基因片段和PKS基因片段用地高辛標(biāo)記為探針從Pseudomonassp.No.2663的基因文庫(kù)約10，000個(gè)克隆中篩選，分離得到的粘粒涵蓋了染色體約95kb的區(qū)域。DNA順序分析了83.5kb的染色體區(qū)域，GC含量為65.43%。生物信息學(xué)分析包含了20個(gè)開(kāi)放讀碼框。其中含有6個(gè)PKS模塊，說(shuō)明FR901464的生物合成采用PKS機(jī)制。同時(shí)序列分析還顯示該生物合成基因簇包含大量新奇的PKS后修飾基因及2個(gè)轉(zhuǎn)錄調(diào)控基因，這為研究聚酮合成類化合物的后修飾提供了一個(gè)很好的平臺(tái)。詳細(xì)的分析結(jié)果列于表1。表1:FR901464的生物合成基因簇中各基因及編碼蛋白的功能分析<table>tableseeoriginaldocumentpage9</column></row><table><table>tableseeoriginaldocumentpage10</column></row><table>3.FR901464的生物合成基因簇邊界的確定根據(jù)基因編碼蛋白的功能分析，F(xiàn)R901464的生物合成基因簇被確定為基因fr9A到fr9T，涵蓋染色體83.5kb的區(qū)域，包含20各開(kāi)放讀碼框。整個(gè)FR901464的生物合成基因簇共20各基因，其中5個(gè)非重復(fù)使用的I型聚酮合成酶基因；1個(gè)雜合的NRPS/PKS的基因；1個(gè)NRPS基因；3個(gè)獨(dú)立的AT轉(zhuǎn)運(yùn)酶基因；8個(gè)與I型聚酮骨架在線修飾及后修飾合成相關(guān)的基因；2個(gè)轉(zhuǎn)錄調(diào)控相關(guān)基因。4.FR901464聚酮/聚肽鏈的生物合成聚酮/聚肽鏈的生物合成相關(guān)基因包括5個(gè)非重復(fù)使用的I型聚酮合成酶基因(fr9E,fr9F,fr9G,fr9H,fr9I)，1個(gè)雜合的NRPS/PKS的基因(fr9C)，1個(gè)NRPS基因(fr9D)和3個(gè)獨(dú)立的AT轉(zhuǎn)運(yùn)酶基因(fr9B，fr9J，fr90)。fr9C編碼的蛋白包括起始模塊(M-L)、延伸模塊1(M-I)和延伸模塊2(M-2)的一個(gè)C結(jié)構(gòu)域(圖3)。起始模塊包含Dtf-KRtGf-ACP三個(gè)結(jié)構(gòu)域，其中Gf識(shí)別1，3-二磷酸甘油酸并轉(zhuǎn)移至活性位點(diǎn)半胱氨酸殘基上形成硫酯，隨后催化水解去除磷酸基并轉(zhuǎn)移至ACP上；Dtf-KR*催化脫水、氧化還原生成三碳起始單位(圖3B)。延伸模塊1包含KS-KR-ACP-KS(失活)-ACP-ACP-TE-KS(失活)-ACP結(jié)構(gòu)域，第二和第三個(gè)KS因催化反應(yīng)的關(guān)鍵的活性位點(diǎn)由C-H-H突變?yōu)镃-A-H和C-N-H(圖4B)而失去脫羧活性。fr9D編碼的蛋白包括NRPS延伸模塊2(M-2)、和PKS延伸模塊3(M-3)的KS結(jié)構(gòu)域(圖3)。M-2包含C-C-A-PCP結(jié)構(gòu)域，兩個(gè)重復(fù)的C均具有活性位點(diǎn)(圖4B)，A識(shí)別蘇氨酸。fr9E編碼的蛋白包括PKS延伸模塊3(M-3)的DH-KR-MT結(jié)構(gòu)域，fr9F編碼的蛋白包括PKS延伸模塊3的ACP-ER結(jié)構(gòu)域、延伸模塊4的KS-DH-KR-ACP結(jié)構(gòu)域、延伸模塊5的KS-DH-KR-MT-ACP結(jié)構(gòu)域及延伸模塊6的KS結(jié)構(gòu)域。fr9G編碼的蛋白包括PKS延伸模塊6的DH-KR-ACP結(jié)構(gòu)域和延伸模塊7的KS-KR-ACP結(jié)構(gòu)域。fr9H編碼的蛋白包括PKS延伸模塊8的KS-ECH-ACP-ACP-ACP結(jié)構(gòu)域及延伸模塊9的KS-OX結(jié)構(gòu)域。fr9I編碼的蛋白包括PKS延伸模塊9的KR-ACP-KS(失活)-DH-ACP-KS(失活)-TE結(jié)構(gòu)域，第二和第三個(gè)KS因催化反應(yīng)的關(guān)鍵的活性位點(diǎn)由C-H-H突變?yōu)镃-Q-H和C-H-A(圖4B)而失去脫羧活性。另外，3個(gè)獨(dú)立的?；D(zhuǎn)運(yùn)酶基因(fr9B，fr9J，fr90)編碼的AT識(shí)別丙二酰輔酶A為底物。具體各蛋白的模塊及結(jié)構(gòu)域組成見(jiàn)表2。表2聚酮/聚肽合成酶的模塊及結(jié)構(gòu)域組成<table>tableseeoriginaldocumentpage11</column></row><table><table>tableseeoriginaldocumentpage12</column></row><table>在上述PKS和NRPS的催化下，1分子來(lái)源于甘油的三碳單位與1分子丙二酰輔酶A、1分子蘇氨酸、7分子丙二酰輔酶A依此經(jīng)活化、縮合生成聚酮/聚肽長(zhǎng)鏈，形成FR901464的分子前體骨架(圖3A)。5.FR901464聚酮/聚肽鏈的在線修飾、解離及后修飾生成的FR901464分子FR901464生物合成基因簇中的修飾基因包括在線修飾的環(huán)合酶基因fr9Q，在線修飾的β-烷基化基因fr9K，fr9L,fr9M,fr9N及后修飾基因fr9P，fr9R,fr9S。首先，延伸至M-4的中間體在環(huán)合酶Fr9Q的催化下化合生成分子中第一個(gè)六元環(huán)(圖3C)。三個(gè)獨(dú)立的?；D(zhuǎn)運(yùn)酶(Fr9B，F(xiàn)r9J，F(xiàn)r90)中的某一個(gè)AT識(shí)別丙二酰輔酶A為底物，并將其轉(zhuǎn)移至獨(dú)立存在的酰基載體蛋白(ACP)Fr9M上形成硫酯，隨后酮基合成酶Fr9N催化脫羧，然后3-羥基-3-甲基戊二酰輔酶A合成酶Fr9K催化該乙酰基硫酯中間體與模塊8中ACP上的碳鏈發(fā)生異戊二烯類的β_烷基化反應(yīng)，隨后乙烯基輔酶A水合酶Fr9L催化脫羧，M-8中的ECH催化脫水形成雙鍵(圖3D)。延伸至M-9的中間體在OX的催化下發(fā)生B-V氧化插入氧原子，隨后發(fā)生鏈傳遞，在DH的催化下發(fā)生脫水，成環(huán)形成第二個(gè)六元環(huán)，TE催化CO2的釋放完成鏈解離(圖3E)。fr9P，fr9R，fr9S編碼的非血紅素鐵依賴的氧化酶、細(xì)胞色素P450羥化酶及乙酰基轉(zhuǎn)移酶依次催化羥化、環(huán)氧化及0-乙?；瓿蒄R901464的生物合成。6.基因置換證明生物合成基因簇與FR901464的生物合成相關(guān)性在克隆、分析了完整FR901464生物合成基因簇，研究了各基因編碼蛋白可能的功能的基礎(chǔ)上，本發(fā)明對(duì)FR901464的生物合成途徑進(jìn)行了初步探討，這有助于理解FR901464的生物合成機(jī)制，同時(shí)為進(jìn)一步通過(guò)對(duì)生物合成基因簇進(jìn)行遺傳修飾獲得FR901464類似物提供了可能。本發(fā)明通過(guò)對(duì)fr9K(編碼3-羥基-3-甲基戊二酰輔酶A合成酶)基因進(jìn)行基因置換獲得的突變菌株不再產(chǎn)生FR901464，從而從遺傳學(xué)水平證明了基因簇是FR901464生物合成所必需的(圖5)。以下進(jìn)一步提供實(shí)施實(shí)例，這些實(shí)施實(shí)例有助于理解本發(fā)明，僅用作說(shuō)明而不限制本發(fā)明的應(yīng)用范圍。實(shí)施例1FR901464產(chǎn)生菌假單胞菌Psuedomonassp.NO.2663總DNA的提取取5-10μ1Psuedomonassp.No.2663菌液(_80°C保存)至50mlLB中，30°C，200rpm，40-50h，至培養(yǎng)基呈現(xiàn)渾濁。50mlPseudomonassp.No.2663菌液，4000rpm，4°C,IOmin,收集菌體；將得到的菌體溶于IOml裂解緩沖液中(含溶菌酶5mg/ml)，渦旋混勻，37°C水浴15min，加入200μ1proteinaseK和Iml10%SDS，混勻后，50°C水浴至透明澄清(大約需要30-45min)，立即置于冰上，加入2.5ml5MKAC,15min后加入Tris飽和酚，輕柔的混勻后，14，000rpm，4°C，15min，上清(用破口的墻頭吸出)用等體積的酚/氯仿抽提2次，上清再用氯仿抽提兩次，上清用兩倍體積的無(wú)水乙醇沉淀15-20min后，用破口的墻頭吸出絮狀的沉淀，用70%的乙醇蕩洗兩次，自然風(fēng)干后，溶于20mlTE中，加入RNase至終濃度為50μg/ml，37°C水浴30min，依次用Tris飽和酚，酚/氯仿，抽提一次，最后用氯仿抽提兩次，上清用兩倍體積的無(wú)水乙醇沉淀15min，破口槍頭吸出沉淀后，用70%乙醇蕩洗沉淀兩次。待沉淀自然風(fēng)干后，溶于1.5mlTE中。實(shí)施例2FR901464產(chǎn)生菌假單胞菌Psuedomonassp.NO.2663遺傳轉(zhuǎn)移系統(tǒng)的建立培養(yǎng)含有適當(dāng)質(zhì)粒的E.coliS17-1至OD6000.6左右，Pseudomonadsp.No.2663，30°C培養(yǎng)至OD6tltl=0.6，兩種菌體按照11這三種比例進(jìn)行混合，用孔徑為0.23μm的濾膜進(jìn)行過(guò)濾，取下濾膜放置在沒(méi)有任何抗生素的LB平板上，37°C分別培養(yǎng)16h后，將濾膜用3mlIOmM的MgSO4洗下生長(zhǎng)的菌落，取適量涂于含適當(dāng)抗生素的LB培養(yǎng)基上，30°C培養(yǎng)約48h，挑取接合子。由于Pseudomonadsp.No.2663對(duì)于卡那霉素和四環(huán)素都敏感，最后確定遺傳轉(zhuǎn)移所用抗生素的濃度卡那霉素50μg/ml，氨卞青霉素100μg/ml。LB(酵母提取物0.5%,胰化蛋白胨1%，NaCl1%，瓊脂1.5%，pH7.0。實(shí)施例3FR901464產(chǎn)生菌假單胞菌Psuedomonassp.NO.2663基因組文庫(kù)的構(gòu)建首先通過(guò)一系列的實(shí)驗(yàn)確定機(jī)械打斷總DNA次數(shù)，在此基礎(chǔ)上確定了機(jī)械打斷得到大小在40kb附近的DNA，用蔗糖密度梯度離心的方法，收集36-40kb的片段，隨后對(duì)DNA片段的末端加上磷酸基團(tuán)，隨后和將試劑盒中帶有的CopyControlpCClF0S-l載體，室溫下連接2小時(shí)，70°C水浴lOmin，使快速連接酶失活。從_80°C取出MaxPlaxLambdaPackagingExtracts包裝試劑盒放到冰上，待其恰好融化，取出25μ1包裝液，立即加入10μ1上述連接液，輕彈混勻，注意不要產(chǎn)生氣泡，于30°C溫浴1.5h；再加入剩下的25μ1包裝液，于30°C溫浴1.5h。加入950μ1包裝稀釋緩沖液(PDB)，上下顛倒混勻；再加入25μ1氯仿，上下顛倒混勻，使其劃過(guò)整個(gè)液體以終止反應(yīng)，用離心機(jī)離心一下，使氯仿沉于底部，4°C保存。將試劑盒所帶的E.coliEPI300-T1K菌種在LB平板上劃線接種進(jìn)行培養(yǎng)。挑單克隆到3mlLB中，37°C，250rpm培養(yǎng)過(guò)夜。再轉(zhuǎn)接500μ1到50mlLB(含IOmMMgSO4)中，37°C，250rpm培養(yǎng)至0D600為0.95。用包裝稀釋緩沖液(PDB)將包裝產(chǎn)物分別稀釋至10°、10_2、10_3、10_4、10_5，取10μ1上述溶液加至100μ1準(zhǔn)備好的Ε.coliEP1300-T1e宿主菌中，37°C培養(yǎng)20min。涂布到LB平板(90mmx90mm)上(含氯霉素12.5μg/ml)，37°C培養(yǎng)過(guò)夜，用于測(cè)定文庫(kù)效價(jià):2xl04cfu/mL·向10μ1包裝液中，加入100μ1準(zhǔn)備好的Ε.coli^^(^^廣宿主菌中，輕輕彈勻，37。c培養(yǎng)20min。涂布到LB平板(90mmx90mm)上(含氯霉素12.5μg/ml)，37°C培養(yǎng)過(guò)夜。每塊平板長(zhǎng)出大約3，000個(gè)克隆，共5塊，用LB刮下，加入甘油(終濃度20%)和氯霉素(終濃度12.5μg/ml)，分裝200μ1—管，于-80°C保存。對(duì)于假單胞菌而言，其染色體DNA的大小約為8Mb，如果插入片段為20kb的文庫(kù)效價(jià)是20005000cfu，就足以代表它的整個(gè)基因組。文庫(kù)的效價(jià)達(dá)到2xl04cfu/ml，具有良好的質(zhì)量，滿足文庫(kù)篩選的需要。實(shí)施例4FR901464產(chǎn)生菌假單胞菌Psuedomonassp.NO.2663的發(fā)酵、產(chǎn)物分離純化與鑒定取0.ImlPsuedomonassp.NO.2663保存的菌液接種于LB(酵母提取物0.5%，胰蛋白胨1%，氯化鈉1%，PH7.0，含有氨卞青霉素50yg/ml)液體培養(yǎng)基中，30°C培養(yǎng)2天后，Iml菌液接種到50mL(500mL錐形瓶)LB培養(yǎng)基中，30°C，200rpm培養(yǎng)兩天后，2.5ml菌液接種到50mL(500mL錐形瓶)發(fā)酵培養(yǎng)基(酵母提取物0.23%，葡萄糖0.26%，磷酸氫二鈉0.24%,硫酸銨0.05%，七水合硫酸鎂0.006%)中，25V，200rpm培養(yǎng)三天，停止培養(yǎng)，離心收集上清，將發(fā)酵液用乙酸乙酯萃取，有機(jī)相用去離子水洗，有機(jī)相濃縮抽干溶于100μL乙酸乙酯。取20μL經(jīng)HPLC檢測(cè)。HPLC分析UV=235nm；柱子GraceSmartRP185μC18Ser.No.0170300853；流動(dòng)相條件V=lmL/min；A=H2O(0.1%TFA)B=CH3CN(0.1%TFA)_時(shí)間(min)05202528B的濃度(％)1550759515_實(shí)施例5PCR克隆FR901464生物合成基因PCR體系包含=MgCl2(25mM)，dNTP(2.5mM)，兼并性引物(40mM)，TaqDNA聚合酶(2.5u)及適量模板Psuedomonassp.NO.2663總DNA。首先95°C，3min，1輪；然后94°C，lmin，65°C，lmin，72°C，2min，5輪；94°C，lmin，60°C，lmin，，72°C，，2min，30輪；最后72°C，10min，l輪。PCR結(jié)束后，瓊脂糖電泳檢查結(jié)果。低熔點(diǎn)膠回收預(yù)期大小的DNA片段，與PMD18vector連接，轉(zhuǎn)化大腸桿菌DH5α感受態(tài)細(xì)胞，涂布在含有氨芐青霉素，IPTG(異丙基硫代_β-D-半乳糖苷)和X-gal(5-溴-4-氯-3-吲哚-β-D-半乳糖苷)的LB平板上進(jìn)行藍(lán)白斑篩選。挑取白色菌落過(guò)夜培養(yǎng)，抽提質(zhì)粒，XbaI和HindIII酶切鑒定是否含有預(yù)期大小的DNA插入片段。插入有預(yù)期大小DNA片段的質(zhì)粒測(cè)序。實(shí)施例6核酸分子雜交1)DIGDNA標(biāo)記將待標(biāo)記的DNA用無(wú)菌水稀釋至總體積15μL，沸水浴中加熱變性10分鐘，立即置于冰鹽浴中冷卻。接著加入2μL引物混合物，2μLdNTP混合物，1μL酶，混合均勻后，37°c水浴約16小時(shí)。加入0.8yL0.8MEDTA(pH8.0)以終止反應(yīng)，加入2.5μL4MLiCl混合均勻，再加入75μL預(yù)冷的無(wú)水乙醇沉淀標(biāo)記后的DNA，置于_80°C沉降40分鐘。4°C，12000rpm離心20分鐘收集DNA，用預(yù)冷的70%乙醇洗滌DNA沉淀，真空干燥后重新溶于50μLTE(ρΗ8.0)中。2)DIGDNA探針標(biāo)記后的質(zhì)量檢測(cè)稀釋標(biāo)記的DNA探針，至以下六個(gè)梯度，1、ΙΟ—1、10_2、10_3、10_4、10_5。稀釋標(biāo)記的對(duì)照DNA至濃度分別為以下濃度1μg/mL,100ng/mL,10ng/mL，lng/mL，0.Ing/mL，0.01ng/mL。分別取1μL上述梯度的DNA樣品點(diǎn)在雜交用的尼龍膜上，根據(jù)7)所述步驟進(jìn)行顯色反應(yīng)，對(duì)比標(biāo)記的DNA探針和DIG標(biāo)記的對(duì)照DNA的顯色強(qiáng)度以決定標(biāo)記的DNA探針濃度。3)菌落雜交(文庫(kù)篩選)的膜轉(zhuǎn)移將保存于-80°C的基因文庫(kù)稍融，取50μL，用450μLLB稀釋得到IO-1的稀釋倍數(shù)，倍比稀釋得到IO-2,10_3，10_4，10_5，10_6。300μL涂平板(15cmX15cm，平板為L(zhǎng)B/12.5μg/mL氯霉素)。選取合適的比例，使每塊平板約1200-1500個(gè)克隆。照選定的比例均勻涂布四塊平板，37°C培養(yǎng)過(guò)夜。根據(jù)平板的大小剪取尼龍膜，小心地覆蓋于平板表面不要產(chǎn)生氣泡，做好位置標(biāo)記，1分鐘后取下尼龍膜置于干燥濾紙上，干燥10分鐘直至菌落結(jié)合在尼龍膜上。原始的平板置于培養(yǎng)箱中4-5hr，使克隆重新生長(zhǎng)作為原平板。將尼龍膜置于變性液(0.25MNaOH,1.5MNaCl)飽和的濾紙上15分鐘(不要浸過(guò)膜)，轉(zhuǎn)移至中和液(1.OMTris.HCl,1.5MNaCl，pH7.5)飽和的濾紙上5分鐘。轉(zhuǎn)移至2XSSC(20xSSC儲(chǔ)備液(廠1):NaCl，175.3g，檸檬酸鈉，88.2g，ρΗ=7.0)飽和的濾紙上自然風(fēng)干。取下尼龍膜置于烘箱中，120°C固定45分鐘。常溫下于3XSSC/0.1%SDS溶液中振蕩洗滌3小時(shí)，以除去細(xì)胞碎片。4)Southern雜交的膜轉(zhuǎn)移DNA樣品在適當(dāng)濃度的瓊脂糖凝膠上電泳至適當(dāng)距離，做好標(biāo)記。浸泡于400mL0.25MHCl中脫嘌呤20分鐘，使溴酚藍(lán)變黃，用去離子水洗數(shù)次。室溫下浸入堿性緩沖液(0.5MNaOH,IMNaCl)15分鐘并輕輕振蕩。換液一次繼續(xù)浸泡凝膠20分鐘，并輕輕振蕩，去離子水洗三次。取一張每邊都比凝膠大Imm的尼龍膜，用去離子水完全浸濕，做好標(biāo)記。采用向上毛細(xì)管轉(zhuǎn)移方法，用10XSSC轉(zhuǎn)移緩沖液轉(zhuǎn)移8-24hr。用2XSSC略微洗膜，120°C烘烤30分鐘。5)預(yù)雜交和雜交預(yù)熱雜交液(20mL/100cm2)至雜交溫度68°C，放入雜交尼龍膜，輕輕振蕩并保溫30分鐘。將DIG標(biāo)記的DNA探針在沸水浴中變性5分鐘，立即置于冰鹽浴中冷卻。冷卻后，將DNA探針與合適體積的DIG雜交液(2.5mL/100cm2)混合均勻。去除預(yù)雜交液并立即把DNA探針/DIG雜交液加入，輕輕振蕩保持雜交溫度64°C或68°C約16小時(shí)。6)雜交后嚴(yán)緊洗脫室溫下用2XSSC/0.1%SDS漂洗兩次，每次5分鐘。68°C，用0.1XSSC/0.1%SDS振蕩漂洗兩次，每次15分鐘。7)顯色反應(yīng)和檢測(cè)嚴(yán)緊洗脫后的尼龍膜在洗滌緩沖液(0.IM馬來(lái)酸，0.15MNaCl，pH=7.5,0.3%(v/v)Tween20)中平衡1-5分鐘，接著在封閉緩沖液(封閉試劑以10%的濃度溶于0.IM馬來(lái)酸，0.15ΜNaCLpH=7.5)中封閉30分鐘，然后在抗體中浸泡30分鐘。用洗滌緩沖液漂洗尼龍膜兩次后，用檢測(cè)緩沖液(0.IMTris-HCl,0.IMNaCl,PH=9.5)中平衡2-5分鐘，最后將尼龍膜置于IOmL新配制的顯色溶液[NBT(硝基氯化四氮唑)溶于70%DMF,濃度為70mg/mL，BCIP(5-溴-4-氯-3-吲哚磷酸)溶于水，濃度為50mg/mL。用時(shí)IOmL顯色溶液中加45μLNBT,35μLBCIP]中，置于黑暗中顯色。顯色合適后用去離子水漂洗以終止反應(yīng)。以下根據(jù)
發(fā)明內(nèi)容提供基因和蛋白序列核苷酸/氨基酸序列表SEQUENCELISTING<110>中國(guó)科學(xué)院上海有機(jī)化學(xué)研究所<120>FR901464生物合成基因簇<170>PatentInversion3.3<210>1<211>83465<212>DNA<213>Psuedomonassp.NO.2663<400>1TTCGGCCGCAGGCGGACGATCTGGTCCAGCTCTATCAGGCGGCGGACGGATGCTGGGTGC60TCGCCATTCTCGAACGGCGTGGTGACGCGGCCGACGTCGTGCTCGATTTCGGCGCGGCCG120AGGTCCTGCTGCAAGCGCAGGACGTGCGCGTGCAGGCGCGCGACCGGCTCGGGCTCGAAG180CCGCGCAGCTGTCGAGCCGCGCGGAAGTGATCACCGAGGCCGCGGCGGAGCGCCACGCGC240ACGTCAGCGGCACCGACGCCACGCATGCCGGCAACACGTTCGTCCATACCGAGGGCCACC300TGGGCATGCACGCGCMAGCGCGGTCGTGACGGCGGAAGCCCTGCTCAAGATGGACGCCG360GCCAGATTCACGTGGGTTGATGCGATTTACGCTTGGAGATTCTCATGTTTGCCAATTGCT420CTGCCGGCGGMTGGCGCTGTCCGGCTCGGACGTCTGCAAGACGCCGCCGCTCGCGATTC480CCGTGTCGTATTCGAACATCGCGAACAAGCCGGAGGCGGTCCCGMCGTCACCMCATCA540TTTACGCGGGCGGTCCCGTCCACAACATGAACACGATCATTCCCGTCACGCACAGCGACG600AGCCGGGATCGATGGGCGGGGTGGCGTCCGGCACCGTGTCGGCGCAGTCGCGCCACGTGA660CGGGGTCCGGCAAGGTACTGATCCAGGGCTCGCCCCAGACCCGGCTGACCGATACCAACC720TGCCCAACAACCAGAACACGTCTGGGCAGACCAGCGCGCCGTCCCAGACGATCACGATGA780CGCTCAGCTGATGCAACCTGTTCGTGCACACAACGGCGAGCCGGGCTCGATGGGCGGGGT840TATGTCGGGCATCGTGTCGACGCAGCCGCGCCACGTGATAGGGGCCGACAAGGTGATGAT900TCATGATGGCGCGCCTCAGACGCGCATGACCGACACGAATCTACCCGCGGCCATGAAGAC960CATCGCGCCGTCCCAGATCATCACGATATCGCTTAGCTGATGMGTCCATTCGTATCCTG1020ATTGCAGGATTGGCGCTGGGTTTGCTGTCGGGCTGCTCGCTGTTCGGTTCGAMCTGGCC1080GACCCGCGCCAGCTGCACGTGACGCTGGTCGGCGGCAGCCGGCTGMCGTCGGCGCGAGC1140GGCGAGCCGCGGCCGATCCAGTCGTGCGTGTACATCGTGCGCGCGGCCGACTGGCTGCCG1200ATGGCGAACGGCGACGCGCCGTGCGCCGCCMGGACCAGGAGGGCGCGGTGCTGACCGTG1260TCGCGCCATGTGATCGCGCCGMCCAGGTGCTGCAGTTCTGGATCGACGTGCCGCGCAGC1320GGCGAGATCTGGCTCGTCGCGGACGCCGACTACGCGCAGCGCCCGGCCCAGTACGCGCCC1380TTGCGCGTGCGCATCGACGGMGCGGGGTGCTTCATCTCGCGGTATGGCTCGATCGCAGC1440GGMTCTACAACGCGTCGTTGCCGGGCGCGGTGCCGCTGCCGCCCGACGCGCCCATCGCC1500GCGAGCGCGCCGMCCCCGACGCCGCMCCGACAMCCCGCCCGACGCCACGGCTCGGGC1560ACGAGGAGATACAGGCMTGAGCAGTCTGCCGGTTGGACCGGTCGCGTGGAGCGACGGCA1620TGCTGATCGAGACGCAGCATTTTCAGCMCTGGAGCGCCACTTGTCCCATCAGGTCGCGT1680CGCGGCTCGGGCMACCGTGAATCACGGCTGGGGCTTCACGCTGCTCGACGTCGACCAGG1740ACGGGCTCGGCCTCGGCCGGCTCGCGCTGCGCGGCGCGCGCGGCGTGTTCCAGGACGGCA1800CTGCATTCTCGCTGCCGTCGCACGACCCGCTGCCCGCGCCGCTCGAGACCGMCGGGCGC1860AGGCGGGCGACATCGCGTGCCTCGCGATCCAGGCCGCGCGCAGCGGCGGCCCGGAMTGG1920CCTTCGGCGAGGTAGCGTCGTCGTCGCGCTACCGGGCGATGACCACCGACGTGCCCGATC1980TCGCGGTCGGCCTCGACGCGCCGGGCACGCCGCGCAGCCTGACGATCGAGACCGGGCAGC2040TGGCGACGCGGCTGTGCTGGAAGTCGCMCTGCGCTCGGACGMGCGGCGCTGCCGATCG2100CCCGCGTGGCCGGGCGCAGCGCGAGCCGCACGGTGCTGCTCGATCCGCGCTTCATCCCGC2160CGCTGCTCGACACGCGCGCGCACGTCGTGCTCMTTCGCTGATCGACGAGCTGCAMGCA2220CGCTGCGCGTGCGCCTCGCGCATACGTCCGGGCAGCGGGTGCTGTCCGCGGGCGGGGGCA2280TCGCGGACCTGATCGAGCTGCTGCTGCGGCAGGCGATCGCCGAGTACCGGATGCGGCTCG2340CGCATCTCGATGCGTTCGATCCGCTGCCGCCCGCGCTGCTGTATCGCGAGCTGATCGGCC2400TGCTCGGGCGGCTGAGCGTGCTGCCCGGCGTCGACGAGGAGCTGGCCGAGCGCGATTTCG2460GCTACCGCCACGACGACCTGCMGGCAGCTTCGAGCCGCTCGCGGCCACCTTGCGGCATG2520CGCTCGCGCGCGTGATCGAGACGCCGGTGCTGCCGCTGCGCTTCGAGGATCGCGGCGACC2580AGGTCCATATCTGCGTGGTCGACMGCAGTGGMCCTGAAGAAGCTCGTGTTCGCGGTGT2640CGGCCGCGATGCCGGCCGACAAGCTGCGCCAGCTGCTGCCGCAGCAGACCMGCTCGGCG2700CGGTCGAACAGATCCAGAAGCTGGTGGACCTGCMCTGCCGGGGGCCCGCCTGATCCCGC2760TGCCCMCCCGCCGCGCCAGATTCCCTATTACGCCCAMGCACCTACTTCGAGGTCGMT2820CGACCGATCCGTTCTGGACGCAGACGCTCGCCGGCTCGGCGATGGCGCTGCGCATCGTCG2880GCGATTTTCCGGATCTGCGTTTCGAAGCCTGGGGCTTGAGAGACGGGMGGTGGCATGAC2940ACACGATATGMCCGCTATCCACGATGMCCTGCTCAGAAACCTTTCCTCCGCCATCCGG3000CGCACGTCGTCGGCCTCCMCCTGATCGACCGGGCGATGACGMTCCCGACCTGATCGCC3060CGGGTGCCCACGTTGTCGTCGCTGGCCCAGGAGGCGTCGGTCGCCGGCGCGGTCACGACC3120GACGCCGTCGGCCCGACCGGCTCCAGCGGCTCCAGCGGCTTCGCCGCGCCACGCGACGCG3180GAGCACGCGGTGCTGCGCTTCCCGACTCCGCCCGGCGCGTTCCGCGCGGCGTACGACGAC3240GGCGCGGACGCCTCGCCCGTCGTGTATACCCCCCAGGGCGGCCAGGTGGCGATCCTGMG3300GCCGGCCAGCAGTCGGCCMCTGGAGCMTCCGTTCGTGTCGCACGCGCTGCCCGCGATC3360CTGCMTTGCAGCGTCATCTCGCGGACGGCCCGCCCGACCAGTCGACGGTGCGCACCCAG3420CTCGGCCTCGMGTGCGCCTGTACCGCGAGCGGCTGGCCGGCTCGGGCTGCGAGTGGGM3480CAGATCCGCGACGCTTCGTATCTGCTGTGCACGTATCTGGACGAGAACGTCAGCGACATC3540GCGCGCGCCGCGTCGCMGTGCTCTACGACGGGGAGCGCAGCCTGCTGGTCGAGTTCCAC3600GACGATGCGTGGGGCGGCGAGGACGCGTTTTCCGATCTCGGCCGCTGGATGMGGCGGAC3660GATCCGCCCATTCGTCTCCTCGCGTTCTACGAGCTGATCCTGTCGCTCGGCTGGCAGGGC3720CGCTACCGCGTGCTCGATCGCGGCGACGTGCTGCTGCAGGACCTGCGTTCGCAGCTGCAT3780GCGCTGATCTGGCATCACGTGCCGCCCGAGCCGCTCGGCACCGGGCTGATCACCCCGGCG3840CGCGCGCGCAGGCGCTGGTGGACGCCGATGCGCGCAGGCGCGGCGGCGCTGGGCGCGGTG3900CTGTTGACCTACGGCATCGTCAGCGCCGTGCTCGACGCGCAGGGCGGGCCGATCCGCCAT3960GCACTCGCGGCCTGGATGCCGCCGACCCGCACCATCMTCTCGCGGAMCGCTGCCGCCG4020CCGCTGCCGCMCTGCTGTCCGAGGGCTGGCTCACGGCATACMGCATCCGCAGGGTTGG4080CTGCTGGTGTTCCGCAGCGACGGCGCGTTCGATGTCGGCAAGGCGCAGGTGCGCACCGAT4140TTCGMCGCAACATCGAGCGGCTCGGCGCGGCGTTCGCGCCGTGGCCGGGCGACCTGGAG4200GTGATCGGTCATACCGATCGGCAGCCGATCCGCACGAGCGAGTTCGGGMCMCCAGGCG4260CTCTCGGMGCCCGGGCGCGCACCGTCGCCGACGAGCTGCGCCGGACCGCGCTGCCGGGC4320GGCGCACAGGCGCCCGGGMCGCCGTGCAGCGGMCATCGGATTTTCGGGGCGCGGCGAC4380ACGCAGCCGATCGATTCGGCGCAGACGCCGGCCGCCTACGMCGGAACCGGCGCGTGGAC4440GTGCTGTGGAAGGTGATCCCGGCGGGCGGCCGGCGCACGGCGGACGCCGACGCGCGCCCC4500GCGCTGCCGGAGGGCGTGGTGATCGCGCCCGACGGCCAGTCGCCGTATGCMCGGAGGAT4560AMCMCCATGATACGMCCAGCCTGAGGGTTCTTGTCGCGATTCTGATCGCCGTCCTGA4620TCTGGTGGGTGGGCCCGCTGTTCGCGCTGGGCATCTACCGGCCGTTCGCGCCGCTCTGGG4680TGCGCGAGGTGCTGGTGGCGGCGGTGCTCGTCTGGGGCCTGTGGCCGGCGCTTGCGCGAC4740TGTGGGCGAGGCTCGCGATGAGCCCGCGCCGGGTGCGCACGCCGCAGCCGGCGGCGCGCG4800CCGATTTCATCGACGAGCGCCTGCGCGACCTGGATCGCCAGTTGMGGCGCGCTGGCAGC4860GCGAGCCGCACGGCCGCCGGCMCGCTGGGCCGGCGCGCTGCGCCGCCAGCACCGGACCC4920TGCTGCCCTGGTATCTGGTGATGGGCGCGGAAGGCAGCGGCAAGACGMCCTGGTCGCGA4980AGGCGGTGMCGCGGCCGGCTCCGAAGGCGAGCGGGCCCTCGCGGTCGAGCCGCTGAACA5040GCCGCGGCGACGACTTCAACTTCCGCATCGCGAGCGACGCGGTCTGGTTCGACATCGGCG5100GGCGCTGGMCCTGCGCGACGGCTTCGACGAGACGGCGCTCGATACCTGGMGCGGTTGT5160TGCAGGGGATGCGTCGCCTGCGCGGCGGGGCGGCGGTGAACGGCGTGGTGTTGTGCGTCG5220ACAGCGCGTCGATGGTCGATGCGCCGCTGGAGGCGCGCAAGCGCCTCGCCGATGTCGTGC5280ATCGCCGGCTGGCGGMCTGCATGAGGTGTTCGGAGAGCAGGCGCAGGTCTATGTCGCGC5340TGAACGGCGTGGACCGGCTCGACGGCGCGGTCTCGATGCTGTCGCTGGTGGACGCGGGCC5400GCTGGGCCAGCGGGATGGGTTTCTGCCTGCCCGAGCAGGCGCTCGGCGAGACGGCCGCCG5460MGCGGGCGCGAGCTGGCAGGGGGCGTTGCAGCACCTGCAGCAGCGGGTCCAGCAGCAGG5520TGCTGTTCGCCGCGCCGGCGGCGACCGAGGTCGGCGCGAACTACGCGCAGCTGCGTTTCG5580TCGAGACGCTGAGCCAGCTGCACMGGCGCTGCTGGTGTGGCTGCAGATCGCGATCGCGC5640CCGGCGAGGCGCATTCGGCCGCGCGTCTGCGCGGCGTCTGGATGGGCTCGATGGCGGAGC5700TGGCGGTGGCCCAGCCGGGCGGGGCGGCGCCCGGGCTGCCCGCGCAGACCCGGCCGCTCG5760GCGAGCTGTGGGCGCCGCTGTTCCGGCAGGTCGCGCTCGAACGCGACGCGGTGCGGCCGA5820GCGGCGTGCGTTCGTGGCGCGGCCGGGTCGGGCACCTGCTGCGCTGGGCCGCGCTGCCCG5880TCCTCGCGCTTGGCTTGCTGATGTGGTTCGGCTGGGGCTATGTCGCGGAGAGCMCTACC5940TCGACGACATCGGCGCGCAGTTCAGCGAGGCGMGCGGCTCGCGCAGACGCAGGCCTCCT6000ACGGCCCGGACGGCGGCGCACCGCTGCTCGACGTGGCGAGCCACATGCGCTATGCGCAGG6060MCAGGCGGAAGACGCCGGGCGCGGGCTGGCGACGCCGTACTTCGAGCATGGCCTCGTCG6120CCGCCGCGGCGCGCGACACTTATCACCGGCAGTTGCAGAAGATGCTGATGCCGGAGTTGT6180ACAACGAGGTGCGGCGCGTGCTGGTCGCGCAGTCGGACGGCGCGCCCGGCGACGTCTACC6240AGACGCTCMGGTCTACCTGATGCTGTGCCGGCCCGATCGGCGCMCGCGACCGATGTCG6300AGCGCTGGCTGAGCAGCCGGTGGGACAGCCTGTCCGGCGGGCAGCAATACGGCGACGACG6360ACCGGCGCGCGCTGCTCGCGCACGTGCGCGCGTTGATGGAGCTGCCGMGCTGCCGGGCA6420CGCCGGAGGATGCGMCCTGGTGCGCGCGGCGCGCGTGCGGGCCGCGCAGATCCCGATCG6480TGACGCGCGTGCTGCAGCACATCCATGCGCAAGGGCTGCCGGCGTCGGTCMCGACATCT6540CGCTGTCGCGCGCGGCCGGCTTCGAGTCGGCGATGAGCCTGCGTATGCGCAGCGACGTCC6600CGGCGACCGACGTCGCGGTATCGGGCTGGTTCACGCGGGCGGGCTACACGGACGTGTTCC6660TGCCGCGTCTCGACMGGCCGCGCGCGCGATGCTCGMGAGGAMGCTGGGTGCTGCGCG6720ACGAMCCCTGCGCGGCAACAGCTTCCAGATCGACGGCGTGGTGCAGMGCTCGCGGATT6780CCACCCGCGCGCAGTTCCTGCAGGACTACATCGCCGGCTGGCAGMCTTCCTCMCGACG6840TGACGGTGCGCAGCGTCACGGGCCTCGACGATGCGTCGCAGCTGGCGGCGGCGATGATGG6900ATTCGCAATCGCCGCTCGCGAGCCTGCTGCGCTTCGCGGCGCGCGAGACGACCCTGACCG6960GCGCGAGCGACGMGGCAACATCGACAGCTGGATCGATCGCCAGMATACCGCTTCGAGA7020AGGGGCGTCGCCAGATCGTCGGCGAACTGAGCGGCCAGCACTACCGCACCGTGCTGCTGC7080CGGMCATGTGGTCGAGGMCACTTCCTGGCAGTCCGGCAACTGGCCGCGCAGCTGAACA7140ACCGCAGCGTGGCGTCGAGCAACCCGCTCTCCCGGTTGTTCGAGCCGCTGTATCGGCAGC7200TGGGGCTCGTCMTGGCGCGCTGCAGGCGGGCCAGGTGCTGCCCGCGCAGTACGACGCGT7260TCTCGCGGCTCMGGAMCCGCCGCGCGGCAGCCCGAGCCGGTGCGGGGCATCATGCTCG7320CAGGATGCGCAGCAGCGTGAGMGACCTACGACTTCMCGCCMCATGMCGACCTGCGG8400CCCACCAACTCGGTCGGCTTCCCGCAGGAAATGCCGTCCAACTTCTTCGCGCCGTTCMG8460ACCGGCAAGTAGGCCGCCCGGACGGGAGCGGTGCACCGTCCTCGGACGTCGCCGTGTTTG8520CGGCGGGGTTCGGGGCTTTCGGGGCGCGGCCGGCGGCAGCCGGCGCGCCCGACCATTCGA8580TAGGAMACAGGATCATGMGAMGTTTTATTCGGACTCCTTGCAGGTGTATTCTCGGTG8640AGCGCCTATGCCCAGTTCGACCAGCTGTTGCAGTCGGTCAAGGATCAGGTGACGAGCACG8700ACGCAGCGGCAGGTGAGCCAGGGCGTGCAGTCGGCGACGAACGATGCGATGCAATCGGCG8760ACGACGCGTGCGCACMGGTGATCCAGTCGGCGCGCACGCCCGACACCGCCGCGAGCMG8820GMGCCTCGGGCGTGCMCCGAGCGGCGTGTTCCAGGAGTCCCGGGGCGCGMGTCGCM8880TGAGGCGGGCCGGGGTGGCGCCCGGCGMCTCCCGAGCCCTTCGGACGCGCCGGACAGTT8940GMAGGGGGAGCACGCATGATGGCATTTTCGATGATGTCGATCATGAGCACGATCGTCGC9000GATTGCGCTGACTTCGCTGATCTTCTCGTTGGGCGTCGTATTGCCCCGGATCATCTCGGC9060CGCGCGGGGCGACGCGATCGTCACGGATGGCGTCAAGGCCGTCGCCGAGGTTGTCGAGAT9120CGGGCAGACGGGGGTGACGCTCMTCAGGTGCCGCAGATGCGGATGGTGCTGCGCATCAG9180CGATCACGGCGTGTCCCGGGACGTGACGATCCGGCAGTACGTCGATCTCGGCAACATGCC9240GCGCGCGGGGGAGCGGGTGAGGGTCGTCATCGATGCACGCGATCCGGCGCGGGTMCCTA9300TCTGGGGCTTGCCAGAGCACAATGATTGCGGTGCCTGCGCCGATTGGCTGTGAAGCGTCA9360ATAGATTGATTCTCGMGCAGGMGTCTGGCCATCGGCGCMGGCCGCCGATGGCGGACC9420GAGMTTTGCGACTCGCCTGCGGGACGTCACTCGCGGTAAGCCGGAATTTTGCCGGGCAC9480TACATTTTGAGTAGCCGGGGTTGTGGCGCTTCGTGCGTCACGACGGTGGCCGTTGACGCG9540MGACAGGTCAGACGGCATGGCTACCGTTCATGCTGTGTTGCTGGGTTCCTGAGATTGAC9600GMCCGATTGCGTTTCGCGCGMCAGCCGATTGCTGATCCTTCGCGGCAGTCGCMCGGG9660GCGGACTGATGGATCGGGCTTCGAGCTTGTATAGGGTGGGMGAGTCAGTGGTTTMATT9720TGGTTCATCGTGGATTACCGGGGMCGCAGCGCGGATTCGGGMGTTGCTCGAAGMGGC9780TCGGGCGGTTTCGCGTGAGCGGCCCTGGCCGATCCATAGCACTTGCCGGCTMGMTTTT9840TCGMCACGTTGTTCGATGTTGTMTCMGCGCCGATGTAGAGGCTCMCATGCACTGM9900TCACGTCATCGGGCCGTTTGCGTAGTGGGGACGCCGCGCGGGGGCGGCCAGGCTCGGATC9960TCTGATAGATGTTCAGTMCGMCTGCCTTCCTAAMTMTCAGGAGAGCGACTGCCGCC10020GCMTGCCTGATACCGGTCACGACGTATTACCTGTTACMGGAGCGGACCATACCTTCAT10080GCTCCTGGGCGCCMCGTGACAMGCCGCCTCGATCAGCTGCGCGGCACTGCGGTCAGTC10140TTGGCTCGCCTCGTTTCTGAATCGATGCAGGATGGGCGAGCCTTTGCGCAGCTCGATCGA10200GACGCAGTCATCGCGCATGCTGCGCGGCAGGCGCTCGACGTCGAGGATCTCGTGCTTGTG10260GGGGGCCAMTAGAGCTGCACGATCGTCGCGATCGGCAGGCCTTTCACGCGAGCGCGCCG10320CGCCGTGMCTCGGTGCGCATGTAGGTGTGGGGCGGAGGCAGCTCTAGCTCTGAGGGCGG10380TTCGCCATGCGTCMTAGTTCTCACTGAGGTTGCCAGCATACCAGGCGCACTGTGCGTGC10440TCGGTGCCTGGCTTCTTGTTCATGGCTCCATTTTGGTGAGGGAGCCTTATGACAGAGAAG10500CTGCTTCGGGGTGCTCGCTGGGTCGCGGGTCCCACTCTCAACTGGGCTCAATGTACTAGA10560CMTGGGCCGACCTTAGCCACCGTTCGGGGTCGGGAMTCCTGACGMTTACTCTCMGG10620GCTGGCGCGTCCACMMCCCGAGGCAGGTCGGCACGCATCGAMCGACAACGATTCGCA10680GAGGAMATTTATAMATAGAMCMTGCATCGATCTGTATCGGTTTTTATCGTGTTTTG10740TGMTGTATATTTCGAAMTTTTCATGAGAMTCTGMGATTTGACTMGTTTCMTGTA10800CATTAGGACACTTACGMTTCCTTGCGCTAACGGTTCGTACGAAMCCATGTCGATCCGC10860ATCGCGTGGTTGCCATCGGCCTTGCATCCAGACACGTCACGGAGGTGTTGCCCGGTAGTC10920AGCCTCAACTCGMGTGATGGGCGGCGGACCTCTCGTCCTGCCGTTGCCGATGGCTGACC10980CGCTATGACGCGTTCGTCCGTCGACCGGMCAGTTGTCGAAATATGGAGATCGTGCMTG11040CATTTCCCATCMTTGCCGCGTCGCGGCAGACGTCTGTCGGCGGCCCATTCGAGCGTCAT11100CGGACGTCTTTGCGTCGCGAGGCGTTGTCCATTGCGGCGTGCCTGACATCGCGCGCCGAG11160GTGGTTCTCGATCGACCGGGGCGGGMGGTTGGCGATGMTCGGGCTACATCCACACGCA11220TGCTGGCGCCAGCATGCGCAGTTATAGATCCATTCGTTCGTGCCGAMTCGAGTGCCCCG11280GACACACAGCGCCGACMGACGTTTCCGGTTCGATCTCATGAGGCTGGCCTTGTGGTGAC11340GAGCTTCTCGGMCTGGCAGCGMCCGCMTGGCCGGTTCCGTGCTGAMTGGTGAGCGC11400CTTGCGACCGTGCTGGAGGCTTTCCGGTGCTTGTCGGACAGGGCCTGCACTATGGTGGAT11460TCATTCGCGCAGTGCATTTTCAMGAGAGACCCMCTGCGCTTGAGCGTGACGTCCTTGC11520GCGCGAGCGCGCCGCTCMGATCGTMTGCAGCATGTCGGTCCACAGCGAGTCGCCTCGA11580CCTGCTGAGCGATGTGTATCMCGTCGACGATTCAGCTCGGGATGCCTGAGCTGCAGTCG11640AGATCGTCTTCCCGGACGAGACTTGCGGACACCTTCCGCCGTGGCCACGAACCMCCCGG11700CMCGCCATCGACCCGCTAGGTTCTTCCTCAGATGTAGGGCGATTGCCCGCTCGACCGGC11760GACGCTCCGCTGCGTTCGCCGCGGACGAGCCGTTACTCATCGGCACAGGCGCCGCGGCGC11820GTGCGTAGACGTTGCTGTGACGATGGCTGATGCCTGGTTTTTCGGCGGTCAGGTTCTTTC11880CGGCGGTCATCATTCCTTCACATTTGACCGGTCCTAMTTCTGACAGGAGCATGTTMM11940ATTCATCMTTAMTAGGTTGATTGATATATTTAGTCATGCAAMTTCGAGGAMTTTGG12000ATGGTTAGCGTATTTACAMACTTGGMGAGTMTATCMACATCGGCACTGAGCGCTTT12060GCTGMGCCCTGCATTCGCTGTTCGTTACCTCCGTAMCGTGGGATCCACCMGATCGCC12120GTGTGGTTCATAMCGAMGAGAGGMGAGATCGTCGGCATGCATCCGTTGGGGGMTTT12180TCCGCCGATGGACGMGTGATATCGACGTGGGTCTGCTGCAGMGTCCGTATTTGATGCT12240GTAGACAGCCGGTTGATGGAMGGGTGTTGATCGCCCGAGAACTACATCTGATCCACTTT12300GGCACATCGGCMCACCTCAMCGGAGTGGCAGAGGCTGCGTTCCTGCGGCTCTGATGGA12360GGCTTCCAGGTGCATCTCGTATCGCGCAMTTGMCCGTCGATACTTGATTTCGCTGTAT12420CGMCCTGTGGMCGCCTGATTATACMTTCAGGAMTGTCGCTTCTGMGAGCTATGCG12480GMGCACTGATGCCGATCGTCGAGATACATGCATCGCACAGGCTGTGCGAATTAGCGCGC12540CGCCCMTGCAGGTGTCGGGCGACGCGGAGAGTTCGATTGGTCATTTGACGGTGCGTCGA12600AMTTTGMTCCAGGCTCAGGAGCGCGGCGATTACGCTGTCCGATCGTGAGATTGMGTG12660TGCGCTGGATTGCTGAGCGGCAGCACATTTCGCGMTTGGCTGATGMCTTGGTGTGAM12720AGCAGCACGATCGAMCGTACATCMGCGTGCGGCMCMAGCTCGGTTTCMGGGGCGC12780CATGGCCTCGTCMGTGGGCGCTAAGCGMTCCTGACAGGGCCTTGAGTAGCCGTGCCGT12840TGAGCACCGGCTGGTTTGTGGTGGCGTGCCGGACATGCTTGCGCCGTTGGCTMGGCATT12900GATTTTGCGCGGAGAGGGTCGTCGCTTCAGTCGGACTTTCTCGCGGCACGGTCATTTCCG12960GTGCGATGACTTGATGCGAGGCTGGGCGATGCGCGGMTTGGCGCCATGGGCGAGAGGGC13020GCACTTGGACCGGTGAGCATTGAGGGTTTGCGTGCCGTTCCGAGTGGCATCACMGGCGC13080GTTCCCGMTCCACGCCGTTGGGGATCAMCCTTACGTACCTGMGGGCGGTGCGAGAGC13140GGCGACCMCACTTCTATTGATCGTGCTCGTCAGCGGCTGGGCCGGCGGACGGCACCAAG13200CAMTATCATGACCTTACGTMGCGMTGGATGATCGCGAGATCGTGTTCATGTATCCCG13260GGCAGGGGGTGCAGTATTTCCAGATGGGCAAGTCTCTTTTCGAGCACGACCGCGTGTTCC13320GCGATAGCCTCATGTCACTCGACCGCATGGTCACGGTCGAGATTGGTACGTCGCTGATCG13380ACTACGTATTCGACGCGGGTCGCGACGTCTCATCGCCGTTCGACATGACGCTGTACTCGA13440ATCAGGCCGTGTTTTGCTTCGAGTATGCGATGACGCMGCGTTGATCTCGCATGGCGTCT13500ATCCCGACCACCTGCTCGGCGCGAGCCTCGGTGMTTGGTGTGCGCGTCCGTGTCTGGTG13560CGCTGAGTCCACGTGACTGCATTGCTTTGCTGGCGGACAGCGCGCGCGTGATGGCGCGCC13620ATTGTGCCCCGGGCGGTATGCTGGCGGTGCTCGCGACTCCGGACCTGTTTGAGCGCGACG13680CCCGGCTGCGTGAGGCTGGCGAGATCGCCTCGATTCCGTCCGCACAGCACTTCGTCATGG13740CAGGGACGCGAGCCGGGATCGACATGGCCGAGGCGCATCTGAGCGTGCTGGGCGAGACGA13800CMTGAGGTTGCCGGTGGAGTACGGTTTCCACTCCTCGGCGATTGATCCGGCACGTGCGC13860CGCTCCGGTTGTGTTTTCAGTCCGTGGCATCGAGCCGGCCGGTGCTGCCGGCCTGGTCGT13920GTCGCACGGGCAGGCCGGTCGAGCGGTACGACGCGGAGCATTTCGACACGGTGCTGCGGG13980ACGTGATCGATTTCMACGAACCATCGMTACATGGAGCGGCGCAGCGCCTGCGTCTATC14040TTGACATCAGTCCATCGGGCACATTGTCGACTGGTTGCCGAACCTTGCTTGAGGAGGCTA14100GCCGATCGGAMCGTTCGCCGTATGCACGCCATTTTCTCGACACCCGACGCTTGATCCCG14160TGCATTGGATGCGCCCGACGCCCGTATCTGTCATGCGCATGCGTACCGCGTMGCGCAGC14220GACGGCGCTATCCATTCCCCTGCCCGMTCGCCGGGCTGCGCGGCATAGCGTTCGGCGGA14280AGGMCGTGCTGACGCGAGCGTGCGACCGCMTATGCGGTCTGCTCGCGCCTGGGGMCG14340CGACGMAGCGAGCTTTCCAAGATTMGGTTGCTTCGGGGTTGAGGGAGTATCAGCCGAG14400TTCCACTTCCTCATGACAMGTGAGGAAMTATTTAGTTTTGCTMTCGAMTGCGTCAT14460CGMTCGTGCCATTTCGACCGCTGTGCGGGGTGTTGTCCCTGGMTTCGGTGCAGACATG14520GTTTGAGAGAGCGTTTMCCTGTCGATGTGGAACCGCATTTTTGMTGAGGTGAMTTGA14580AGCCAMGGTGCCTTTGTGATTGCMTTTTAGCCACGAGAGTCGGTGCGGGCCATCMTC14640AGTCGGTTGGCGGCCTAGGTGMTTGACAGGTCGATCGCACAGGTCTTGTCATCACGCGC14700GCATAGGAGCGAGGTATACGGGTTGCCTCGTCMTGTCCCGGTCGGGCATATCCGCAGCG14760MTGGCGCCGATTTGCCTTCCATCACACTCGCTGCGCCCTGCGCCAAMCGATGCTTGCT14820TTGGTCGTGGTCGTGGTCGTGGTCGCGTGCCGACGTTCCGTTTGTCCATGCATTGAGGCA14880TCGGGCCGGTTTGCTCGCGGGCTGCTCCGCGCCAGATTGTCCTCTCGCATCACCTGGTTC14940GACCGTGCATCCCCTGCTCGMCAGCGGCCTGACMCGCCGCTGCCGGACCATCGTTCGC15000CCAGCTTGGGCGAGTATGTTCGCCGGTCCCGCACTTTTCCAGGTGCGCTCGGCMCGAGT15060ATCGMTCACGTGCGAGCCATTGCCATGAGMATGCCGATCCGATTCATCTTGATGTGCG15120GTTTACGCMATGGACGTCGMGCTTTCGCTCGACTCAGCCGGGATTGCAATCCTCTGCA15180CATCGATCCGGATTACGCACGGAGATCGACGTTTGGAGGATGCATCGTCCATGGAGCGAT15240TGGGGTGCTGGCCGCGCTTGATCGGATCGGGCTCGCGGMAGGGTTCGCGTCACCAGCCT15300GTCGGCCAGTTTCMGMTCCGATGTTTGTCGACCGGACCTATACGTGCCMGTCGAGGT15360GCAGGGTGACMCTGCCGCACTGTGACCTTGCGCGACGGTACMCGCTGCTGACGCAGAT15420CGGAGTTACATGGGCGCCMTCGATGGTGAGGAGCCAGCGTCTCACACGGMCCCGCGCT15480TGCCAGCAGCGTTGMMTATCGTTGACCACGACGACGGCGCACTGGMGCGGGCATCGA15540ACTGTCCGGCGMTATCCGCCGACCGGCCTCGGMCATGCTATCCCCGTGCGCTTCGCAT15600GTTCGGCGAGACCGTGCTGGACACGATCGCGTTATGCAGTTTCCTGACGGGGATGAMCT15660GCCCGGCCGTCGAGCGCTGTTCTCGGCGCTGACGCTGACATTCGMCATCGAGAGTCAGA15720TGGGGCGCTGMCTATCTGCATGCGACTCAGTCGTTCCATCGCGACTACGGTTTGCTCGA15780GACACGGCTTGTGGTCGGCGACGCACACACGACGATCGCACAGGGCGMTTGCGGTCCTT15840CGTCAGGMGACGATCGTGGACCTGCCGGTACAGGGCTATCTCACGGCGCGGTCCGATTC15900GGACGCGATACGGCTGGCGGGCMGGTCGCATTCGTGACAGGCGGCTCGCGTGGTCTCGG15960GGCGGCGCTCGTGCGATGTCTCGCGGTGCTCGGATGCCACGTCTATCTGAATTATCTTGG16020CAGTGCCGCGCATGCGCGTGCCGTCGTCGACGATCTTGMTCGCATGGTCTTTCGGTCAC16080GTTGATCMGGGTGATGTCGGCGATCGCGCTTGGCTCGACGTCGTGCGMTGGMCTGCT16140TGCTCGACACGGCCGCCTCGACATACTGATCTGCMTGCCTGCGAGCCGCCGCTGCGGAT16200GGAGGACGATGCGCCGTCGTTCGCGGTGCGCCGCGMCACTATCTTTCGCGCMCCTGCA16260ACTCGTCMCGTTCCGCTCGCGGTTTTCTCCGATCTGTTGGCGGCGAGTCMGGTGTTTG16320TGTTGCGATATCGTCGGAGATGGTCGACAGCGTACCGGCCGGCTACGCCCACTACGTCGA16380TCTGMGCGGCAMCAGAGGCATGCGTACGACACCTCGCCGACACGCMCCGTCGATTCG16440CACACTGATTGCGCGGCCGCCGMTTTGCTGACGGAGATGAGCMCACGCCGACACGCAT16500CATCMTGCGCACCCGCCTAACGAGATCGCMTGCGGATCGTCMTGCACTGCCGGTGGG16560MGCGATCGTCATCACCTGCTGGCGAGTTTCACGGACGATGCGACACCGCGCGCGCCGGA16620GACGCTCATGGTGACCGCCAGCTTCGCGCTCGATGCGATGCTCGGGGAGTTCGATGGCTG16680GCTCACGTGTCTGCCAGGTGAGCAGGCGGCGCGGCAGACCGGCTACMCCGGATGATGCA16740GGACTTGCTCAGCCCGGACAGTGTGCTGMTATCGCTGMGGCACGGTGAATCTGCTGTA16800TTTGCGCGTCTCGGACTGGCTGCATGATCGAGAGGCCTGCTGGCMGATCMGGGCTGCG16860TTTATCGGMGAGGGGGATTTCATACGGCGCGTCGTGGACGATTTTGTCGCCGCGCTGCA16920TGGCTTCGCGGMCGTAGCCGCGCCTGGGAATTGCTGATTGTTTGCCCGGATCCGCGCCT16980CGATCCGGCCGATGTGGCAGGCGCCGAGCTACTCGCTACGGTGCATGCGAGGTTGCGCTC17040ACTGCCCGTCGAGCTGCCGGGACTCACCACCGTGTTCGCATGCGATCATCATGCGCTGCA17100CCGGATCGACGCGACGCGCATCGACGATGCGTCACGCMCCGTATCGCACACATCCCCTA17160CGTGCAGGACTATTTCGGCTTCCTCGCGTTACTGGCGATCCGGATGCTGAACGCCCGGCG17220CCAGCTGCCCGCGMGGTAGTGGTGCTCGATTGTGACMCACGCTGTGGCACGGCGTCTG17280TGGCGAGGMAGCGATGTCGTCMGCTCTTGATCGACGACGAGATGCGCGCGTTTCAAM17340GTCGATGAMGCGCTGCTCGCGCGAGGTTTCATCCTGTGTCTTTGCAGCAAGMTCGGGA17400GGMGATGTCTGGMGGTATTCGACCAGCATCCAGACATGATCCTCACCCGCGAGGACAT17460CACTGCCGCGMGATCMCTGGCTGCCGMATCGGACMTCTACGCATGCTGTCCGATGA17520ACTCGGACTTGGCATGMCAGCTTCGTGTTCTTCGACGACAATCCACTTGMTGCGCGGA17580GGTTCGAGCCGGTGCGCCCGAGGTGTTGACCGTCTGCACGACGCAMGCGGGATTGCTCC17640ATCGGMTGGGCTGMCGGCTCTGGGTTTTCGACCMCTGACGCACACGCGCGAGGATAG17700CGCACGTGCGCGGATGTATCGTGAGCAGTCCGAGCGGGMCGGTTCCGGCMTCGACAGA17760GTCGTTTCGCGTCTTCATCGACGGGTTGMTCTCMCGTCGCGATCGAGCCGCTTACGGC17820TGGCGGCGTCGATCGCGCGTCGCAGATGACCATGCGCACGAATCAGTTCAACMCACGAC17880GATCCGGCGCGACMGGCGGAMTCATCGCGATGATGMCGATGACACGTGGCGCCTGGT17940CACGATCGACGTCATCGACCGGTTTGGTGACTACGGGACCGCCGGCTTCGTCGCGTACCG18000GATCGMTTATCGTGCCTCTACGTCGATGCTTTTCTGCTCAGTTGCCGCGTGCTCGGTCG18060CACGGTCGAGGATCGCGTGGCGCGATATCTTGCCACGGTCGCGGGGCAGCATGGCGTCGA18120GCATATCCACGTTGCCTTCGTGTCGACCCGCAAGMTATGCCGTTCCAGCMTTTCTGGA18180CAMCTCGCACCGTCGGAGATCATGGTTGACGACGAGGCGCGCGGCGAGACGGGGCGCTA18240TGTATTCGMCTCGGCGCGCTGCGCGAMCGCTCGATCGGCAGGCTTGGCTCGAGCACGG18300GCCTCGGGCGGMCCCGATGGTGCTTCCTCGGCGCCGCCGTTCGTCGTCGGCGCGAGTCC18360GCCCATCCMGGCACCGACTATGTGGGGATTGCGGMCGGTCGMTCGAGCGTTCGACGC18420MTCGGCGCATCGGGCGACGAGGAGCCCGCGTCCGGCGCCGCCCTTTTGCCGGATACCTT18480GGCGTGCGGCGAMCCGAGTTGGTTGCCGCCGCGAGTGGGGCTGAGCACGAGGCGGCGTT18540GTTGCGTGAGGTCMGCTGTGCTACGCGCGCGTGCTGTCGATCGATCCTGACACTTTGTC18600CGMTATGAGGMTTGGAGCGATACGGCCTTGMTCGATCGATGTCGTMATCTGACCGT18660CGMCTGACCCGTCTCGTTCCCAGGCTTGGGCCGACGTTTCTGTTTGCGCACCGCACGCT18720GCGCGATGTGGTGCGCGGCCTGATTGCCGAATACGACGCGAGGGCTCCGGGCGTCATGCC18780CGMCCGMGMGGCAACCGTGGGATCGGCGGMTCGMCATCCGATCGGACGAGGCGGA18840CGAGCATCACGACGACATCGCGATAGTCGGGCTGCACGGTGTTTATCCGGGTGCTGACGA18900CATGGAGGCATTCTGGCMTTGCTGCGGGCGGGCGAGAGCCATATCGTGCCGATGCCCCT18960TGCMGGCGGCATTTGATCGATCGTGCGCTGCCTGGCGTCGCGTCGACGTTGCACGGCTA19020CATGGACCGCGCCGGCTACCTCGACAGCATCGMTGCTTCGAGGCTGMCTGTTCGGCAT19080CACGCCGMGGMGCCGAGCTGATGGACCCGCAGCAGCGCTTGTTTCTGCAGGTCGTCTG19140GGGATTGCTCGAGGACGCCGGCTATACCCGCCAGATGCTCGATCGCGCGACCGGCGTCTT19200CGTCGGCGTGTTGTCGAGTGACTACGCGATGTACGCGMTCTGCATGCGTTGCAGTACCC19260CGAGCMTATCGCCATACGGATTATTACCAGATCGCGMCCGCGTCTCGTATCACTTCGA19320TCTCMTGGGCCMGCATGTCGATCGATGCGGCCTGCGCGTCATCGGGCACCGCATTTCA19380TCTCGCCTGCCAGGCGATTCGAGACGGCGACTGTGMTCCGCGATTGTCGGCGGCGTCM19440CCTGATCCTGCACCCGAGCCGGCTGGTCCAGTACACCCAGACGGGCATGCTGTCCGGCAG19500CGGCAGTTGCACGCCGTTCGGGCATGACGCGGACGGCACGCTACTCGGGGAGGGGATTGG19560CGCAGTGCTGCTCMGCGCTTGTCTCGCGCACGTGTTGACGGCGATMCATCCACGCCGT19620CGTGMGGCATCGTCAGTCAACAGCGGCGGCAAGACCMTGGCTTCACGGTGCCGMTCC19680GMTGCGCAGGCGAGCCTGATCGCGGGCGCGCTGCGCGCGTCGCGCGTGCCGGTCCAGCA19740GATTGCCTATGTCGAGGCGCATGGTACCGGCACCGCGCTTGGCGATCCGATCGAGGTCGG19800CGCGCTTGGCCGCGCGTTTCGCGAACTGGGCGCMCGGGCTCCCGGCGCATCGGCTCCGT19860AMGGCGMTGTCGGCCATGGCGAGTCGMTGCGTTTCTCGCGAGCCTGAGCAMGTCCT19920GCTCCMTTCCGTCATCGTCAGTGGGTGCCGACGCTGACTTCGGCGCMGACMCACGGC19980GATCGATTTTTCCGCGGCCGGCTTCGAGGTGCAGCGCACGCTTGCCGACTGGCCCGTATT20040GTCAGACGGCGMTCGGTGTCGGCTTGCATCAGCMCTTCGGTGCGGGTGGGTCGMTGC20100GCATGTGGTGTTGCAGGCTTGGCCGGCMGCGCCGGAGMGGTCGCGCGGGACGCCATCT20160CATACCATTGTCCGCCCATCGGCCGGCCCTCTTGCCGCGCCTCGCGCGCCGCTTGCGCGA20220TCGCTTGATGCGCGATGATCCGGCTACGATCGATCTCGATGCGGTCGCATATACCCTGCA20280AGTCGGCCGCCATCATCTCGACCATCGCGTCGTGTTCGTGGTTGACAGCCGCGACGCGCT20340GCTCGATGCGCTCGAGCGTTTCCAGGGGGGCGTCACCAGCGACGCGCGTTGGATGTGCGG20400CAGCACGACCGTGCGTMCCGGCTTGGCGAGCTTCTCMTGAGCMGTGGACATGCGTGC20460GCTGTTGTACMGTGGGGCCGTGAACMGCGCATCATCAGATCGCTCAGCTATGGCTGM20520CGGGCTCAGTATTCCCTGGGATGCCCTCCATGAGACGCGCAAGCCGGTGCGCGTCAGTCT20580GCCGACCTATCCATTCGATGGCAGCCCGCACTGGCTGGAGCACACGCACGCGGCCGTCM20640GTTTGGCGCCGMTCGTCCGCATCCTCATCCGCATCCGCATCCGCCGCCGACCCGGCGCT20700GTTCGCGCCGACGTGGACTCGGGCGGATCTGCCGACTGCGAGCGTTTCGATGTCGGACCT20760CGTCGCGCATCACATCGTGTTTTGCGGCATTGMGCCGATGCGCAGGACGCGTTGCGGAC20820CGCGAGCCGAACGCACGACACGAGGGTGCATTTCGTGCCGCAGACGGCGCGACCGTCGGA20880GACGGACTACGCCGGGCMGCGACCGCGCTGATGGGTTGGTTGCGCGAGTGGCTGCGGGA20940GGGGTTGCGCGAGAGACTGCATCTGCAGGTTACGGTTGTCGGGGCGGGAGATGAGGCATG21000GACGGGGCTCGCAGCGCTTCTGMGTCGGCCATGCAGGMAATCCGCTGCTGTCCGCGCA21060ACTTGTCGCATTCGATGAGGCACCTGATGCGACGGCATGGTTCGCTCGACTGCGTGACGA21120MTGCGGATCGGCCACGATGTATCGATCAGCTATCGCCCCGAGCGTCACGTGCGGACCTG21180GTATCCGCTGGCCCCGCCGGCTCGGCCGTTCTTCGAGGGCTTGGCGGGGGTGGCCGATGG21240CGCAGGCGCCTATCTGATCACCGGCGGATCGGGCGCGCTTGCGCTGCMTGCGCCGMTG21300GCTCTGCGAGCGGGATCGCGACACGACGATCGTCCTGCTGGCGCGTCGCGACGAGGCCGA21360GCTGATGCGCTCGGCGCAGGGGCCGCGCTTCGACGCATTGCGCGCCGCCGGGCGGCGGAT21420CGCCTACCGCAGCGTCGACGTATGCGACGGACMGCACTCGATGCGTGCGTTGCAGAGGT21480CGTTGCGACGCACGGTCCGATCCGGGGCGTGCTGCATTGCGCGGGCGTMTCCGCGACGG21540CTTCCTCATCCACMGTCCGAGGATGACTTCCAGGCCGTGCTGGCGCCGAAGGTGGGCGG21600CGTGTTGMTTTGGATCGGGCGACCCTCGACCAGCCGTTGGCGTTCTTCGCACTGTTCTC21660GTCGATCTCCGGTGTGCTTGGCMTGCAGGTCAGGCCGACTATGCGGCCGCCMTGCTTT21720CATGGATGCGTTCGCGCATCGTCGTGCGGCTCGCCAGCGCGCGGGCGMCGGACCGGGCG21780CAGCGTCTCGATCMCTGGCCGCTTTGGCGCGGCGGCGGAATGATGCTCGATGCCGMCT21840GGCGACGCTGCTGCGCGMCAGCACAGGCTCGACGCGATCACCGGCGMGCCGGGATGCG21900CGCGCTCGACCGCGCGCTGGCCTCCGATATCCMCAGGTATTCGTCGGMCCGGGGCAGC21960CGATGCGGTTGCACACACGCTGGCCGCCTCCGATCGTCGCCMCCCGACGATGCCGCCGG22020TACGGCGGCGCCGCTCGAGGCGGAATCAGCGACGAGCGCGCCGMCGCCGCCGACCTGTA22080TCCGTATCTGMGCGCATGCTCGCTGATGCGACGMGCTCGATCCCGTGACGATCGATGA22140ACATCGTCCACTCGCGGACCTCGGTCTCGACTCGATCCTGCTCGCGMGCTGMCMGGT22200ACTGGCCGTCMCTTCCGTCMCTGCCGAGGACGCTGTTCTACGAGTATCCMCGCTGGC22260GGCGCTTGCCGCATACCTGGCCGAGCAGATCCGCTATCAGGGGATACCGGCCGAGCGCTT22320CGCGGCAGCGCGCGCGACGGTGGAGCAGCCCGCGCCGGCTATCGCGACGAGCCCTGCCGG22380CGGCGAGCCGACCCGTCGCCCCCTGAMTCCGGCGCGAGCGGAGCGAGAGGCGTCGACGA22440TCCCATTGCGATCATTGGCATCGACGGACGTTATCCCMTGCACCATCCTTCGACGCGTT22500CTGGCAGCGCCTGMGACCGAGTCGAGCGAGATCGGTGMCTGCCGGTTCACCGCTGGCA22560CGGCCCGGGCGCGGATGCGGAGCGCGATCTCCACCCGACGCTGCGTMCCGGAMGGGGC22620GTTCCTCGACGACTTCGCTCAGTTCGATCCGACGTTCTTTCAGATCGCGCCGATCGACGC22680TTACTCGATCGATCCGCAGGAGCGTTTGGTGCTGATGTCGTGCTGGACCGCGCTTGMAG22740CAGCGGCTACACGCGCGMCGTATCGCGTCGCAGCATGGCCGCMTGTCGCAGTCTGCGT22800CGGCGCGACCMGAGCGGCTTCMTCAGTCTTCGTCGGGAACGGTTCCGCGTGCGGACGA22860CATGGACGCAGGCGCGCGCGMCCCGTCACATCGACCTCGTTCGCGMCATGGCGMTCG22920CGTGTCGTATTTCCTCGACTTGCACGGGCCGAGCATCGCCTTCGATACGATGTGCACCTC22980CTCGCTGACGGCGATCCATCATGCTTGCCAGTATCTACGGCMGGCGAGGTCGMCTCGC23040GATCGCGGCCGGCGTGMCCTGTACCTTCATCCGMCGATTATCTCGCACTGGATCGTCT23100CGGCATGCTCAGCGACGMGCGCGTGTGMCTGCTTCTCACTCGGCGGGAACGGATTCAT23160TCCGGGCGAGGCGGTCGGCGCCGTCGTGCTCAAGCGTCTGGATAGGGCGCTCGCGGACGG23220CGACCGGATCGACGGGGTCATCACTGCGACAGGCATCAGTCACAGCGGGCGCACGCATGG23280CTATACCGCGCCGAGCGTCACGCAGCAGGAMCCCTGATCGGCCGCGTCATCGMCGCGC23340CGGCAGGCACATCGAGGAGATTGCCTACATCGAGTCGGCTGCGMTGGATCGAGCCTGGG23400CGATGCGGTCGMTGGGGCGCGCTGAAAMGGTGTTTCATTCGCGTACGCMCCGTGCCC23460GATCGGTTCGGTCMGCCGAACATCGGTCATCCGGAGGCGGCGGCCGGCATCGTGCAGCT23520GAGCMGGTCATCGCGCAGTTGCGGCATCGGGCGCTTGCGCCGACGCTTGCCGACTCGTC23580GCGGCTGGACCACGATTTGCTTGCCGACAGCGGATTGCGGCTCGTCACGCGCTATGAGCC23640GTGGCCGGAGAGCGGCCCGTCCGCCTGCCTGATCACGTCCGCCGGTGCTGGCGGGACCTA23700CGCGGCCATGGTGGTCGAGCCTGCGCCCGTMCCGGCGACGCGCCGGTCGMCGTGCGGA23760TGCCGTGGCGATTGCCTTGTCCGCGCGCTCCAGCGAGGCCTTGCGCCGCGTTGCAGCGGA23820ACTGCACGACCAGCTCGCGGCCTATCCCGATCTGCGGATCGAGGACGTGGCTCGMCGCT23880GCACATGGGCCGTGAGGCMTGMGGTGCGCACGGGCTGCGTTGTCGAGAGTCGCCGGGC23940GTTGATGAGCCGGCTGCGCGCGATGACCTCGGGCGAGCCGGCGGGCGAGGCGGACGCATC24000TCGGGACACCGTCGCGACGGTGCCGCTGCGCGTCCGGCTCGMGCGGCGCTGAGGGTGTG24060GCTGGCGGGGCACGATGTCGACTGGCCGGCAGCTTACGGCGATGCATGGGCGAGTGCGCA24120TGTCGCGCCGCGCGCGTTGCCCGGCTACCCGTTCGACACGCGTGAGTATTGGCGCGAGCC24180GTCGCACGCGCGCCGCGCGGGCGGCCTCGTGCCTGCCCGGCGCGTCTTGCCGGCGCTGCC24240GCCCGCCGTCGCCACGGCTTCGGTTCCGCTGTCGGCCGTGCCCTCCCGCGCGCAGATCCA24300GGCGGCGCCGAGTGCGATCCTCGCCGMGTGAAGMTGCGATTCGCGCATTGCTGCATGT24360TGMCCGGCCGAGGAGATCGACGATGMGCGACGTTCTTCGAGCTAGGTGTCGATTCGAT24420CCGGATGGTGTCGTTCGTGAGGGGCATGGCCAGTCGATTGTCGATCGCACTCGATGMAC24480CGCGCTGTTCGATGTTCCGACCGTCGCGCGATTCAGCGCGCATCTCGCGCGCTTGCGCGG24540GGCGCGGGACGCGCCGGATGCGCTTGACACCGCCGCCAGCCCCCTGGTGCCGACCGCGCG24600GGACGATCGTACGCGCGTCGCGATTGGCGGGCCGGATGMGGCGTGATCCTGCGGCAGCT24660GMGACCGTCGTCGCGGTGATCCTGGACTATCCCGATCCCGCCGTGCTTGATGATGACGT24720GACGTTCTTCGMCTCGGTGTGGACTCCATTCGGATGGTCGGGTTCGTCGAGGATTTGTC24780CGCACGACTCGCGCTCGACCTCGAGGAGACGGTGCTGTTCGATTATCCGACGCTTGCCGC24840CATGGCGCGTCACGTCGCCACGCGMTGCAGGTCCGAMCACGGCGGCGACAGCAGCCCC24900TGCCTCGCGCGAGGTACCGGCCGAGACGCGTCGGGMGCGGCCGGCGGACCGGACGCGGC24960GCCGTTGGGGCATGAGGCTTTGCTGMCMCCTGCGTCGTCACGGTCGTACCTACGMGG25020GGTCGTGCCGCTCCMACCGAGGGCGATGGTCCGTTGGTGTTCTGCCTGCATCCGGCGTC25080GGGCGACGTCGCCATGTTCGGCMGCTCGCCATGGCCGCCGGCGACCGCTTCCGCGTGAT25140TGGTCTCMGGCGCGGGGCTTCCTGACCMTCAGCCGCACTGCGCCGACGCGGAGGAGAT25200GGCGCGCTACCACGCGGAGATCATCCAGGCGATCGATCCGGTCGGGCCGTATCACGTGAT25260GGGCGCATCGATGGGCGGGGTGGTTGCCTACGAGGTCGCGCGCGCGCTGCMGATGCCGG25320CCGACMGTGCGGACGGTATTCATGCTCGAGGCACCTCTTGTCACATGCGAGCGCGACTC25380GGATATCTTCACGCTCGACGCGTTGCAGMCTGGTTGGCGAACGCGMTTTCCTGATGAT25440CGCGATGCTGCACCTCGACCCCGCATTCCGTCAGAGAAMGAGGCCGGGCTCGTCAMTG25500GGATGACCTCGTGATCACGGCGATCGMCTGGGGCTGGACGAMGCGCGCTCCGAGACGA25560TTCGCMGTTGAGTCGCGGCTTGTCGATCTGATCGTCGGGCGCGGGGTGCGGCAMCGCC25620GGGMTTTTGCGCTCGCGCTTGCGTTCGATGGCGGACACGCATCGCMCAACCTGCGCTG25680CATGCGCGMTATCGTTGGCGTCCGCTTGTTCGACCTGACGAGGTCGAGATCCTGATGTT25740CCGCACAATGACCGCGCGAGCGACGTCGGATCAGGTCTATAACCCGMCTACCTCGAGM25800CCTTCAGCGCGTACACGGCTCGATGATGCCGATGCTCGACGCCTGGGTCGAGCGGATGTC25860GCAGGTCCGGACCGTGGTGGTCGGGGGCGAGGATCATTTCGATCTCTGCAATCGGACCCT25920CGGCGACGMGCGGTGATAGGGCCGATGCTGGCATCGATGCGGCGGGCGGAGCCGGMCC25980GGMCCTGAGGATCAGGGCGACACCAGGTCGAGCGCATTTCGGGTGGCCGTGATCGGCAT26040CTCGGCGCGCTTTCCGGGTGCCGATCATGTCGACGCATTCTGGMTGTGCTGGCCTCGGG26100CGAGAGCCTGATTCGCGCGGTGCCGGCCGAACGCTTCGGGCTCCAGGATGATGCACCGGA26160CCACGGCTGCCTGTTCGAGCGCATCGACGAGTTCGACCATGCCTTTTTCAATCTGAGTCC26220GMCGAGGCGCGGATGATGGAGCCGGCCGAACGCATGTTCTTGCAGGAGGCCTGGCATGC26280CGTCGAGGATGCGGGCCTCGTGCCCGATCGGGGCGCCGGCGAGMTTGGGGCGTGTTTGC26340CGGCAGCGGGGGCGACTACAACCTGCATGTGCAGTCGGTACTCGGCATCGCGCCGCAGGT26400TTCGACGTCGACCGTGCCGGGACGGGTCGCTTATCAGCTGAATCTGCATGGGCCCACGAT26460GACGATTGACGCCGGCTGCGCGTCGTCGCTGTTGGCGGTGGGGCAGGCATGCGACAGTCT26520GCGGCTTGGCCAGTGCGAGGTCGCGATCGCGGGGGGCGCGATGGTGTATTCGACGCGCM26580TCTGATCGCAACCGGTCACTACTACCGTCTGCTCAGCMGACGGCGCGTGGCGCCGCGCT26640CGATGCCGGCGCGACCGGCATGCTGCCGGGCGAGGCGGTCGGCGCGGTGGTGCTGMGCC26700TCTCGACGCGGCACTGCGCGATGGTGACCGTATCTACGCCGTACTCGATGCGTGGGGCAG26760CMCCACMTGGCCGTACCCAGGGCATCGCGGCGCCGAGCGCCGCCTCGCMGCCGCGCT26820GCTGACCTCGATCCACCGCCGCTTCGGCATCGATCCCMCACGGTCGACTTCTTCGMGC26880CMCGCGAGCGGCGCACCGCTTGGCGACMAGTCGAGTTGCAGGGACTTCGCATGGTGTT26940CGGCGGGCGCCCCCGCGCGCATCCGCTTGCGCTCGGCACCGTCGAGMTACGGTCGGCCA27000TTCGTTTGCCGCATCAGGCATGGCGCATCTGCTGMGGTGATCCTCGCACTACATCGCGA27060GATGCTGCCGCCGACCGCGAACGTCGATGCCGCGACGGCCGTTGCGGGCGGCGACGATGG27120GCTGTTCGAGATCCCGGCGCGTGCTCGGCCCTGGCCGTCCGGGACGGGCGCCGCACGCCG27180CGCGGGCGTCAGTTCGTATAGCGCGACCGGCATCMCGTGCATCTGGTCGTGTCCGAGGC27240ACCGCCGGCCGCAGCCGCGACATCCGTCACGGGGCCGACGCTGCTTGCGTTCTCCGCGGC27300GACGCCCGAGGCACTACGACAGCGTGGCCGCGATCTGCTCGATTATGTGGCGGCGATGGA27360CGATCMTCCTGGGCACCGGCGCGGCTGTCGGCGCACTTGCTTTCGCGGCGTCTTCACTT27420CCGTCATCGCTGTGCGTTGCTTGCCCGTGATCGCGCCGATGCCGGGCGCTTGCTGGCGCG27480CTTTGTCGCGMCGAGGCGGATGCCGAGGTGCTGACGMCACGGTCGGCGCGACGACGGA27540TGACGATCCCACGCGCGAGGCCCTGCGACGTGMGCCGCGGCATTCGGAGCMCGCGCGC27600GGCGCTGCCGCTTGATCGGGCTGCGCAGTGGTACGTGCATGACATCGCGTTCGATGCGCG27660AGGCGGCTTCGCCTCGGTCGATCGCGTGCCGCTGTCGCTGCCCGGCTATCCGTTCGCACG27720CAGCCGGCACTGGCTCGAGGCGTCGCCGGTCGCGCAGGATGTTGCCGCGCAGCCGGTCAC27780GGMGATCCAAGGCACTGGCTACTGCGGCAGATTGCGGTGATCACAGGCGMGTTCCGGA27840CGCGGCGCAGGCCGAGGCGAGTTGGAGTGCGATGGGTTTCGACTCGCTGATGAGCATCCG27900CCTGCTGTCGACGATCCAGGMCAGACCGGCATCGAMTTCCACTCGTCGATTTGCTCGA27960CCAGTCCACACCGGCGGCATTGCTTGCGCACCTCGACGCCCTCGGCGCGTTCGCGCATGG28020GCAGTGCGGCGAGGCGCCGGGACCGGACACGTCACCCTTGCCGCCGGTGCAGTATGCGTG28080GTTCGGCGATCGCCTCGCGGCCCTGCGCGCGACGGCCGMGTTGCTACGGTCGCGCTGCG28140CCCGCMGCGCCGACGCCCGCATCGCGATCGCATCGCGATCGCATGCTCGCGGAGCTGCT28200CGATGCCGGCATCGCGGTGTTCCGCGACACGGACAMGCAGTGCTGCTTGCCGCGCGGTC28260GGTTGGGATCGATGCGCGGTTGCGTCGTCTCGACCAGGACACACTCGCCGCGTGCCTCGG28320CATGCTCGAGCCGGGGCAGCTCTGGGCACCGTTGTCGCAGGMCAGGCGCGGGATCTGCA28380TTGGACGGAGCGGATGGGTAGCACCGCATGGAACGTGTTCCACGTGTTCCGTCTGTATGC28440CCACGMGTCGACCTCGMCGTCTACGCCATGCATTGACCTTGCTTGCTCAGGAGCAGGA28500CCTGCTGCGCACGCGCTATATMCGGTCGGCGACGGCCATGCGCAGTGCGTATCGGMCT28560CGTCTGTGTCTCGCTCGACTCGATCTCGATCGACAGCAGCGAGGCGTTCCATCMTTCAT28620CCAMGTCGCCGCAGTCATCCCCTTCTCGAGGAGCACGGCGGTCCGGCMTCCGGATGTG28680GCTCACGGAGGTGGACGGCGCGTTCCATCTCGGCATGGTCGCTCATCACGCGCATGCCGA28740TGCGTTCACGCCGGGACTGCTTTTCTCGCAGCTCATGGCGTTGTACGGCAGCCTGGGGAG28800CGAGGCGGGCGCGCGTGACGACACCCGGCGGTGGGCCGATCAGTACTGGTTCTATGCACT28860GCGCCAGCACGACGCGCGCGGCGCCAGCGCCGATGCGCATCGCGCCTGGTGGCGCGATAC28920GCTCGCGGGCGTCGAGGTCGAGATGCGTCTGCCATACCGCAAGGAGGTGACGTCCTCGCC28980GTCGTTGCGCGCGCTCGCCACGMTCAGATCGTGCCTGTATCGGCGGAGCTTGGTGAGCG29040GATCMGGACCTGAGCCGCGTGCACGGCATCTCCTATCCGCAGCTATTCGCGGCCGTGCT29100GGCGATTGTGCTGCGTGAGTGTTTCGGCMCGATCGTCCGGTGCTGCAGGTGATCCACM29160CCAGCGCGATAGCGCGGCACTGGCCGCCTGCCTCGGCGATTTCTCCMTGTCGCCTTCGT29220GCCGTTCGAGCTCGACCCGGGCCGCAGCGTGATCGACACGCTGTACGCGGTGCGCCGGGT29280TCTATTGACCGCGCTGCAGCACACACGTGTGCCCATGGCCGATCTGTTCCGGATCGCCGG29340GGTGGACGGCCACACCGGTTACGGCGCGATGGTTGGCGACGTGGTGCTCGACAGCGCCGA29400CATGGATACGGCCMCGTCAACATCGCCGGTGMCACGGCGTTTCGACCTACATGMCGC29460AGTGCTCGMCAGCGCAGCATCCATTTCCTCGATCAGGCGCTTGCGTCGCTGTTCTTCCA29520ATTTCTGAGAATCGATGGACGTCTCTACATCTTTCATGCGTACCGCCGCGCCCTGTTTGA29580ACAGGMACGATGCMMGCTCCCGCMTTGATCGTGGAGCTGGCCGACGCGATGCTGCG29640MCGCCTGATGTTCCGGTCGCGGATCTCATTGCCGCGTTCGGCCGGCGCTTCGATGAGCT29700GGGCCGTGGCGGACGGGCGCCGACGATGCGCGACGCACCCGCCGCCGCCGTGACGGGCGA29760TTCGGCGGCGCTGCTCGMCGGTTCCTCGGCGGCGATCTGTCGATCGCCCMCTGCGCGG29820CTCATTGCATCCGGCCGGMCGCGGGACAGGGGGAGAGTCGTCGCCGMTGAGGCGGGGC29880GAMCGAAGCAGCGTGCGGGTTGGTGTGCGGGACGCGCGCAACGACCGCGGGAGGGGGAG29940TTTCATGAGGCGAGGGCATGATGGGAGCGGTTCMTGAGCGACGCAMGAGACTGGCGGC30000ACCCGGGMCGTCGAGGATGGCMGCGGGAGATCGTGCCGACGGAGGAGGTTGATGCATG30060GCTGMGCGGCACTTCATGGCCGATGCCCAAGCGCCGCTGTCGCGTMCCAGAGAGGTAT30120CTGGTCGCGGCAMTGTTGTACCCGATGGACCCGCTCTATAACGTAGCCGTTGCCTGCAC30180GGTCGGCGCCGGGTTCGACAGGGGGCTGTTCCGGCGCGTCTGTCAGCGGTTCCTGMTCA30240TCACCMCTGCTCMCGCGCGCATCGTCGCCGAGGGCGACGCGCTGATACAGCGCATCGA30300TTCCATGCGGGMCTGGCATGGTACGMTCCGCGACCGGCGAGTCGCTCGACGCTTGCCG30360CGCGCACGCGMGCGTGACGCCGCCGTGCCGTTCGTGCTCCACGACGGACCGCTCTACCG30420MTCCATATCTATGMGCGTCGTCGTGTGAMCGGTCGTGCTGCTCGTGGTGCATCACAT30480CATCTGCGATGGCAGCTCCGTCAGCCGCTGGCTGTCMCGATCTGGCAGGACTACCGCGC30540GCTGMGCAGCAGCGCACGCTGCCGCAGGCGCTGGGCGGCGCGAGCTTCCTCGATTTCGT30600GCAGCGCGAGGCCGCGCATCTCGACTCGGCAGCGGGACAGAAGAGTCTGGCCTACTGGCG30660CACGCAGTGCGCTGATGGCTTGCCGCCGCTCGACGCGGCGCTCMCCTCCCGCCCGCCGC30720CGATGACGCATCGGTATTCGCGACCAGCCGGCTGTCCTTTTCGATCGACGGCGATCGCGC30780CATGCGGATGCGCACGCTGCGCMGACCGGTTCGTTCAGTCCATCCACCCTGCTGATGGG30840GGCTTTTACGCTCGCACTGTCGGCGTGGCGCGGTACGCGGCGCTTCACACTCGGTCTGCC30900GACGCTCGGCCGCCCCCCAGGCGCAGAGTTCGCGTCGACGATCGGCMTTTCACGMTCT30960CGTGCCCGTGGTCTGTGATCTCMTCCGGCGCAGACGTTTTCGCMTGGCTCGACGGCTG31020CCAGCGTTACGTCTACCGCGCGCTCGATCACGGATATCCGTTCGCCGGACTGCTGCAGGC31080GCTGCCGCGCCATGCGGATGCGCCGCTGTCGATCCAGACCGTGTTCACTTACCAGAMTT31140CGGCGATGAGATCGACATCGGCACACTGCCGGATCGGGTCGGCGATTCGGTCGMCTTGC31200CTTCGGCGCTGATATTTTTCAGCGCGACGAATATCCGGTTACCTGTGMGTCGTCGAGGC31260CGGCGATGGATGGACCGTCAACCTGAMTACAAGACCGCGGTGATCGMCATGACGACGC31320GGAGGCGCTGCGACACGCATTCGTCGCCGTGCTCGACGATGCGCTCACGGACCCTGACCG31380TACGATCGGCGCGCTGACGGCGCGCGATGATCGGCGTCACGGCGCCGGCCCGATGCAGCC31440GGCATCGCATGGTCCTGCCGCGGATGCAMGAGCCTTTGCGCGCGCTTCACGGCGCTCAG31500TCGCCGGGATCCCGCGCGACGCGCGCTGAGCTTCGATGGCCGCCACATGACCTACGGCGA31560CCTCGACCGCGGCAGCGACGCGCTGGCCCGGCATCTCGTGTCGCTCGGCGTGCGGCCGGG31620CGACCTGGTGTTGCTGTGCGTCACGCCATCGCCTGATCTGATCACCGCGATCCTCGCGAT31680CCTCMGGCCGGCGCCGCCTACGTACCCATCGATCCGGGCTCGCCGGCGCAGCGTATTGA31740MTGATCCGCGAGGACAGCGGCGCACGCTTCGTCGTCACGACCTCGATCTCGGAGGCGGC31800GGTCGMAGCGTCGCGCACCGTGTCGTGCTCGATCGGGACGGCGCGGCGATCGCTGCGGC31860GGTCGAGCGCGAGCCGACCCTCGTGCTGCCCGATGTCCGACGCGGCGATCTCGCCTACGT31920GATCTACACCTCGGGCACCACGGGCAMCCGAAGGGCGTGATGATCGAGCATGGCMCGT31980GCTGCGTTTGTTTGACCGCACAMCCATTGGTTCGACTTTGGCCCGGACGATGTCTGGAC32040GCTGTTTCATTCCGCTGCCTTCGATTTCTCGGTCTGGGAGATCTGGGGCGCGCTGCTTTA32100CGGCGGCCGTCTCGTGATCCTGGACACGACGCAGCGCMGGATCCGGTGCGGCTGCTCGA32160GACGCTGGCCGATGAGCMGTGACTGTGTTCAATCAGACACCGGCCGCATTCTACGCAGT32220CGTGCGCGCGGCGACGGCGGGCCCGCCGCGGCGCCCATTGGCGTTGCGCCTTGTCGTGTT32280CGGCGGCGAGAGGCTCGACTTCGGGCGACTCGCGCCATGGATCGAGCGCTACGGAGATAG32340CCGGCCGGCGTTGATAMCATGTACGGGATCACCGAGACCACCGTCCACGTCACCCATGC32400GCCGGTGACAGCCGCCGATGTGACGGGCGCGTGCGCCTCGGTGATCGGTCATCCGATTCC32460CGACCTCGACGTGCMTTGCTCGACGAGCACCGTGCGCCGGTGCCGGATGGCGAGATCGG32520CGAMTGTACGTCGGCGGGGCGGGCGTCGCGCGCGGCTATCTAMCCGGCCCGMCTCAC32580ACAGGAGCGCTTTGTCACGATATCCGGCGTGCGCTGCTACAAGACAGGCGACCTGGCCCG32640CCGCMTGCGCGCGGCGMCTCGAGTACATCGGCCGAMCGACGCACAGGTGMGATTCG32700CGGCTACCGCATCGMCTGGGCGAMTCGAAGCGTGCATGAATCAGCACCCGGTCGTCGA32760GCAGGGCGTCGTCATTGCGCACGAACACGCCAACGGCACGAMCATCTCGTCGCCTATTA32820CCGTGCGCGGGACACGGCGACGGACGATGAGGCGCGATCGCATGATCTGCGCGCCTTCGT32880CGCCGCCAGATTACCCGMTACATGGTGCCGGCCTTCTTTGTCGCGCTCGCGGMCTCCC32940GTTGACGGGCMCGGGMGTTCGACAGGCAATCGCTGAGCCGGCGGCCGATCGTGCTTGC33000CTCGACCGATAGCGACGTAGCCGGCGCCAGCGCGACCCAGCAGCGGATCCGCGMCTCTG33060GCAGCAGGTGCTTGGCGTGGCGCCGGCGCACTTCGATCAGGGCTTCTTCGAGGCGGGCGG33120CGATTCGCTGTCCGTGGTGCTGCTCGCGCGCGCCATCGMCAGGCATTCGGTCACGCGTT33180TACGCCCGCGMGCTGTTCCGATATGCMCGGTGCGTGATATCGCGGCCTATCTGGAGAC33240ACACGCGACGCGCCCTGACTCGAGCGACGCGGCGGTGTCCGTCGGACCGCGCGCGACGGC33300GGCTGACGAGTTGTCGCGCGCCTCGCCCGTCGTGCCCGACTATTACGACGAGAGCCTCGC33360GATCATCGGTATCTCGTGCGMTTTCCGGGCGCCGCGGACCATCTCGMTTTTGGGCGM33420CCTGTTGGCCGGTCGGGAGAGCGTGGAGTGGCTGACGCGCGCCGCGCTCGACGMGCGGG33480TGTCGCGCCCGATCTCGCGAGCCATCCCGATTATGTGTCGGCGCGTTTCACGATTCCCGG33540CMGACCCGCTTCGACGCCGATTTCTTCMGCTGTCGGCGAAGCTCGCCGMTTCGCCGA33600TCCGCAGTCGCGCCAGTTGCTGATGCATGCATGGMGGCCGTCGAGGATGCCGGCTATGT33660GCCGGGGCMTTCGACGCCACCGGCGTATTCGTCTCGACCGGCMCAGCGGCTATCAGAC33720GTTGCTCGACMCGCCCCCGATATCGAGCAACMGACCGCTACCTCGCCTACGTGATGTC33780GCAGGGCGGCTCGACGGCGGCGCTGATTTCCTATCAGCTCGGCCTGACCGGCCCGAGCAT33840GCAMTCGGCACCMTTGCTCATCGTCGCTGGTTGCGATCGACGCGGCGTTCMGAGTCT33900GCGGGCGGGCGMTGCCGGCAGGCGATCGTCGGCGCGTCGACACTGTTCCCGCACGCCM33960TGCTGGATTTCTCTACCAGCCGGGTCTCMCTTCTCGAGTACGGGGCATTGCCGGACCTT34020TGATGTCGATGCCGATGGCATGATGGCGGGCGAGGGCGTCGCGGTGATCGTGTTGMGM34080CGCGCGGCAGGCCGTCGAGGATGGCGACGCGATCTACGCGGTCCTGCGCAGCATCGCCGT34140CMCMTGACGGCCGCCAGAAGAGCGGCTTCTACGCGCCGAGCGCGCAGGGGCAGGGCCG34200TGTGATTCGGACGGCCCTCGMCGCGCGGCTGACATCGACGTCGAGTCGATTGCTTACGT34260CGAGGCGCATGGCACCGGCACGCGGCTCGGTGATCCTGTCGAGGTGATGGCGATCGACGA34320GGTCTATCGGCAGCGGACCGGGGCTCGGCAATTCTGCGCGATCGGCTCGGTGMGCCGM34380CATCGGCCATCTGGACACGGCGGCGGGGCTGGCCGGCTGCATCMGGTCGCACTGGCGTT34440GCGGCATCGGATGATTCCACCGACCATCMCGTCACCACGCCGMCCCGGCGATCGACTG34500GGCGTCGTCGTGCTTTCGCGTGCCGCATGAGGCGTCGCCGTGGCCCGAGGGCGCATGTCC34560GCCACGCGCAGCCCTCAGTTCATTCGGACTCGGCGGCACCAACGCGCATGCGATTCTCGA34620GGCCTGCCCGGACGTCCGGGCCGCGTCGCCCGATAGGGACGCGCCGACGGGCGAGCCTCG34680ATTGGTGCTGTTGTCGGCCGCCGAACCACGGCTGCTGCCGACGATGGCGCGCACGCTGTC34740GGTGTTCCTCGTCGTGCATCCGGACTTGCCGTTCGACGCAGTGGCCGCCACGCTGCMCG34800TGGCCGCCAGCCGATGCGGCACCGGCTCGCGATCATCGCAACCGGCGCGCAGGAGGCCGC34860CGAGCTGCTCGAGCGCTATGCAGCCGMGCGATAGAGGGCGCGACGGCGGCGCCGTGGCC34920TGCCGCGATCGTGTGCTCGCGGCCCGACGCCGCGCCATCCGCGCCGGGCGCCGCGATCTG34980TCGGCGCTGGCCGCGCTGTGGGTCGAGGGGCACGATGTCGACTGGTCGCTCCTGCATGCC35040GGCGCGGCGATGCGCCGCGTGCATTTGCCGACCTATCCGTTCGCACGCCGGCGCTATGAG35100GTCGCGGCCCGGTCCACGGCCGCCGGGACGGCGGGGCCGCGCCTCGCCGTTCAGCTCGCG35160ACGGACGGCTCGAGGCAGGTCCACATCCGGCTGCGCCGCGACGACGACTACCTCGACGAC35220CATCGCGTCGACGGCCGCCGCGTGCTGCCCGCCGTGATGACCTTCGMCTGCTCCGCGCG35280GCCGCGATGACCGATGCGGCCGTTCCCGACGAGGCMGGTTGCAGATCGAGCACGTCGTC35340TGGTTGCGGCCGGTGATCTGCGATGGTGAGGCGCTGTCGCTGTGCGTCGAGCTGGCCGCG35400CGCGGTGTCGGACMGACTGGCGTGCCCCTGTCGCGTTCCGGTTGACGGGCGTCGATGAC35460GCGACGAATGAGCGGGTCATGTTCGCCGAGGGTGCGCTCGCGTTTGAGGCMGCGGCAM35520GTGGACGGCACGGACGGGTGGATCGCGCCCGAGGCGTTGCGGCMGCGCTMCCGAMAC35580CGGATCGACGGGGCCGACTGGTATCGCGGTATCGCGGCCGGGCGAGTCCAGTACGGGCCG35640ACCTACCTCGGCCTCGTCGAAGCGGGCTGGMCGACATGGGACTGACCGCGCGCGTGCGA35700CTGCCCGAGTCGGTGTCCAGCTCGCTCGCCACGTTCCCGCTGCATCCGGCGCTGCTCGAC35760TGCGCGTTGCAGGCGAGCGTTCTCCTGGACAGGGTCCGGCGTCATGGTGGTGACCCCGGG35820CAGTGGTCCGGGATGATGCCGGCGAGGCAGMGCTGCCGTTCTTCGTCGAGCGCGTCGAC35880GTGCTCGCGCCGCTCGMGCGGMGTGTGGGTGCGGGTACAGGGCCACGAACCGGTTGCG35940ACGGCGCGCGAGGCGCGGTACGACATCTTCGTTCATGGCGCCTCTGGCGCATTGCTGGTC36000GCGATACGCGMTTCGTCACACGGGCGCTGCCCGGGGGGAACGMGACCGAGCGCTACCC36060GCGCCMACCCCATCGAGATGGCCGCGCAGCAGAAAMTGACGCCGATACTGCCCCTCTG36120CACACGCTGACGATTGCCTGGACCCCGCTCGATGAGAGGCGCGAGGCCGGCGTCTCGACG36180GCGCCCATCGTGCTGTTCGGCATCGGCGAGGTTCCGCCCGCCGAGCTTGTCGCGTGTTTC36240GCGGGCCGTGTCGTGCGCTATGTGCCGTTGCGCTCGGAGCCGGATGGTCCGGCGCTGGCC36300GCGCTGCCCGCCGACGCCGCGCTGGTGTTTGCGCTGCCGGCAGGCGCTGCGCGCATCGAC36360GACGCGGCGCTGATCGACGCACAGCGCGACGGCGTGGTCGCGGTATTTCGCTGGTTGCGG36420GCGCTGATCGGCCACGGCTTCGAGCAGGTCGACCTCGACTGCACCTTCGTCGTCGAGCGC36480ACGCMCGGGTGCGCGCGGGTGATTCGATTGCACCGGTGCATGCCGGTCTGATCGGCTTG36540GCGCAGAGCCTCGCGGGTGAGTATGGGCATTGGCGCGTGCGTCTGCTCGATGTGGGCGGG36600TTTGCCGACCTGCCGACGCCAGCGGGCTGGCCAMCGCGGAGGGCMGGTCGTATTCGCC36660TGGCGCGACGGCCGCTGGTTCACGCGGAGCCTCGTCGAGACGGCCATGCCCACGTCGTGT36720CCMCGCTATACCGGCMGGCGGTCACTATGTGGTGCTGGGCGGTGCGGGCGGCATTGGC36780CGCACGTGGACCGAGACGATGATCCGAGACTATCGCGCGCGCGTGGTCTGGCTGGGCCGA36840MGCCGCGCAATCGCGAGATCGACGATGAGATCGCGCGCCTCGGCMGCTCGGTCCCGCG36900CCGATCTACCTGCMGCCGATGCGACCGACCCGGCCTCGCTGCGCCGCGCGCGGCATCGC36960CTCACCGAGCTGGGTCTGGACACGCTGCACGGTGTTGTCCATTCGGCACTGGTGCTCGAC37020GATCGGGGCATCGCGGCCATGAGCGACGCGCAATTCGCGCGCGCGCTCGACGCCMGGTC37080GCGACATCCGTGMTCTCGCACGCGTGTTCGGCGACGGCGATCTCGACTTCATGCTGTTT37140TACTCCGGCACGATCTCGTTCGTGMCCCTGCCGGCCMTCGMTTATGCGGCCGGCTGC37200AGCTTCCAGGATGCCTATGCGATGGCGTTGGCCGACCAGCGGCGATGTGCTGTCMGCTC37260GTCCACTGGGGGTACTGGGGGCATGTCGGGGCCGTCGCGACGCCGGMTACCGCCGCCGG37320ATGGCGGCGGCCGGCGTCGGATCGGTCGMCCCGMGCCGCMTGCMCGACTGGCTCM37380TTCATGCAGGACMTCATAGGCGGCAMTCGCCATCGMTCTCGTTTGCGTGCTCGCGGC37440GCGGCTATCGACACGCCGCAGGCGACGGATGCGCGGCCCGCCGGCACGCCCACGGACGCT37500CATCGTTCGCATCCGGACGCGCAGGGTTGGAGCCGGCTATTGCAGCGTGTCGACGCGCTG37560CGCGAGCACAACGGCCTGCGCGATCCAGTGGTCGAGCACGCGGCGTTGCTGATGGTGCGC37620CAGGTACTCGGCGCGCTTGACGCGCTGCCGGTCGATCGMCGCACGACGGCGCGTACCGG37680GCCGGACCGCGMGTCGCCTCGCCGCGATTTATGAGCGCTGGTATCAGGAMGCTGCCGG37740CAGCTCCAGCTCCACGCGTTGATCGACGTGTCGGGCGGAGCGACAGGCCACGCMCGCGT37800GCGACGGMGACGCCCATGCGGCTTGGGCGAGGGTGCGCGACCATTATCGTGCGCATCTA37860AGCGTTCGGGCCGGTATCGAGCTGATGGACGCCTGCCTGCGCMTCTGCCGGCGATTCTG37920CGGGGTGAGCTTGCCGCGACCACGGTGATGTTCCCCGACTCGTCGATGCGGCTCGTCGM37980MCGTGCATCGCMTCATCCGATCGCAGACTATTTCMCGAGGGTGTCGCCGMGCGGTG38040ATCGACCACGTTCGTCATCGCGTGGCGCGCGAGCGCGGCGTGCGCTTGCGGATTCTCGAG38100ATCGGGGCCGGCACGGGCGGGACCAGCAGCMGGTATTGCCGMCCTGCTGCCGTGGCGC38160GAGCAGATCGMGMTACCGCTATACCGATCTGTCGMGTCGTTCCTGTTCTATGCCGAG38220GATACATACGGAGCAGATGCGCCGTTTCTGMMCAGCGATTCTCGACATCGMGCGCCG38280ACCACGGATGACCATCCGCATGCGGACMGTTCGGMGCTACGACATGGTGATCGCCGCC38340MTGTCCTGCACGCGACGCGCGATATCGGCCGCACGTTGCGCCATGCAMGGCGTTCATG38400CGCGACGGTGGCGTGCTTGTGCTGMCGAGATGAGCGAGAACTGTGCGTTCTCTCATCTG38460ACCTTTGGGTTGTTGGAGGGCTGGTGGCGCTACGACGACGAGCCGGTGCGCATCCCGGGA38520TGCCCGGGCTTGTCTCCGCTCGMTGGCGGCGCCMCTCGAGCGCGCGGGCTTTGTCGAT38580GTGCAGTTCCCCCTCGGCGACGCACAGCCGCTCGGCCAGCAGGTGATTGTCGCGCMTGC38640CGGCMCGCGCATGGTTGCGCGCCGAGCGCGATGMGCCCGCGGCGCGGCCGccGcGCac38700cGCCACCGCGCCTGGCTCCGGCGCCGCGCTCGMCCGATACTCGCGACGCTGCTCGCGGA38760AGTCCTGCGGATCGACCCGGCCGATGTCGATCACGMCGCAGCTTCTCCGACATCGGACT38820TGACTCGATTCTCGCGATTCMCTGACCMTAGGCTCMTGCGCGCTTGGGMTCCAGCT38880GGGCACGATCGAGCTGTTCGATCACMCACCGTCGCGCAGCTCGCCGCGCATCTGACGM38940CCTACATCGCGTGACCGAGGCGGCGGGCGGCTCGGCGCCAGCTCCCGCGCCGTGCGMAC39000GGCGACCMTGCGGCGGCTGGACCGTCCTCGCGGGCTGCCACGGGCGATGTACTCGMCG39060TGTGACGGTGATGCTGCGGGAGCACGTTGCGCGCGCGGTGCGTGTCCGCGAGGACGAGAT39120CGATTTGCATCGCAGCTTCGCCGATCAGGGCGTTGATTCGATCATCAGCGTGCAGATCGT39180GMCCGGATCMCMGTTGTTCGGCCTGCGCATCGCGACGACGGTGATGTTTGATCACM39240CACCGTCGGCCGGCTTGCGGCCCACCTGTGCGAGCGACACGGCGCGGCGCTTGCGAMGC39300CGTGCAGACCGMGCGAGCACGCCACCGGATGCGGCCGCGCCCGATGTCGCGCCCGCACG39360CGCCGCCGCGACGGCGCTGTCGGCGCGCGAATCCGMCCGAGCCGMCGGCGAGCGCGCC39420GCCGGCCGCCGGCCCAGGGTCGATATGCGCAGTCCTGATCGATGGACCCGGCGAGATCGA39480AGATCTGTCGATCGCCGCTGTCGCATCGCGTGGGCTCGATCACGATGMGTACGCGTGGC39540GGTGCGCGCGTTCTCGCTGAACTTCTCGGATCTGCTGTGCGCGCGCGGGCTCTATCCGM39600CATGCCGCCGTATCCATTCGTGCCGGGCMTGAGGCAGCCGGTGTCGTGATCGAGGTCGG39660GGCGGGCGTCAGCACGTTCCGACCCGGCGATGAGGTCGTCTGCCTGTGCCAGGGCTGCCA39720TGCGGAGCAGGTCATCTGCACGACMGCCAGGTATTCGCCAAGCCCGCGAGCTGGAGCTT39780CGAGGMGCCTGCGCGTTGCCGATCGTCGGCATCACGATGATCGACGCATTCAGCMGGC39840CGCGCTCGCCCGCGGCGAGCGGATACTGATTCAGACCGCGACAGGCGGCACCGGGCTGAT39900CGCGGTGCMCTCGCGATGCACCGCGGCGCGGAMTCTTCGCGACGGCCGGTAGTGAGGC39960CMGCTGCGCTATCTGCGTGATCTCGGTATCGCGCATACGATCMCTATCGMCGGAGGA40020TTTCGCCCAGGMCTCGCGCGGCTGGCGCCGGGTGGAGTCGACGTCGTGATCMCACGCT40080CGGTGGCGATGCACTCCAGAAGGGGATCGACTCTCTCGGACCGGGTGGCCGCTACATCGA40140GATCGCGATGACCTCGCTGCGGAGCGCACGCAGCATAGACCTGTCGTCGCTATCCGACM40200CCAGACTTTCTACAGCGTCGATCTCGCGCGCATGGGGGCACGGGATCCGCGGMGATCGC40260GCGGTATTGGGATGAGCTTGTGTCGCTGACAGAGCAGGGCGTCGTCCGGCCGACGATTTC40320GGAGGTCGTCGCGTTCCGTGACGTCCATGATGCCTATCGTCGTCTTGCCGATCGCGGGM40380TATCGGCMGATCGTCGTGCGCGTCGACCAGTCGAGCGCGCCCCGGCCGCGCGMCGCGA40440GCGTGGAGCGMCCTGCCGGCGCGGGACGCTGCGGCGTCCGGCGCGCCGGACGGTGCGAC40500CGMCCGGCAGGCGCGGGCGCGCGCGTGGTACGCGACGACGACATCGCGATCATCGGGAT40560GAGCGGCCGCTTCGCGCGCTCGCCCGACCTCGACGCATTCTGGTCCCACCTCGCGTCCGG40620CACCAGCCTGATCGCGCCCGTGACGCGCTGGGATCTCGGCGACGGCGACGCGCGTTGCGA40680GACCGGCAGCTTCCTGGACGATCTGTCCACATTCGATCCGTTCTTCTTCAAGATCTCGGG40740GACCGAGGCGCGCTACATGGACCCGCAGCAGCGCCTGTTCCTGGMGMGCGTGGMGGC40800GCTCGAGGATGCCGGCCACGCCGGCGAGTTGGATMGCAGCGACGCTGCGGCGTCTACGT40860CGGCTGCTCGGGCGGCGACTATCATCTATTGTTCCCCGGGCCCGGCGCATCCGACGGCGA40920CACGCGCGCCCGTCCGCCCATTCAATCGTACTGGGGCMTGCGGTGTCGGTCATTCCGGC40980TCGCATCTCCTACTTCCTCGACCTGCACGGCCCGGCCGTCGCGATCGACACGGCCTGCTC41040GAGTTCGCTCGTCGCGCTGCACATGGCGTGCGCCGCGCTGCGCAGCGGCGAGGTCTCCAT41100GGCGCTGTGCGGCGGCGTGTTCGTGCMTGCACGGCCGGCTTCTACCAGCAGGCGCAGCG41160CGCCGGCATGCTGTCGACGACCGGCMCTGCTATGCGTTCGACGCGCGTGCCGACGGCTT41220TGTGCCGGGCGAGGGGGTGGGCGCGATCGTGTTGMGCGGCTTGGCGACGCGATCGCGGA41280CGGGGATCACATCGATGGCGTGATCCTCGCGTCGGGCATCAATCAGGACGGCAGCACCM41340CGGCATCACGGCGCCTAGCGCGCGCTCGCAGGAGGMCTCGAMCCGACGTCTACGATCG41400CTTCGGTATCGACCCGCGCGAGCTGCAGATGGTCGAGGCGCACGGGACGGGCACCCCACT41460CGGCGACCCGATAGAGTTCACCGCGCTCGCCAACGCGTTTGGCCGCTACACGCGGGATCG41520CGGCTTCTGCGCGCTCGGCTCGGTGMGACCAATATCGGGCACACGACGACGACGGCGGG41580TATCGCTGGCGTGCTGMGATCTTGCTCGCGCTCCGTCATCGCCMCTGCCGCCGTCATG41640CMTTTCGMCGCCCCMTCCCGAGATCGAGCTTGACGACAGCCCGTTCTACGTCMTAC41700ACGCCTACGTCCTTGGCGCGTCGAGACCGGCCGCCGCCGGATGGCGGCGGTCAGCTCGTT41760CGGCTTCAGCGGCACCMTGCGCATGTGGTGATCGCCGAGGCGCCACCCATCGCGCTCGG41820GMCCCGAGGTCCGCGCCGGGCCAGCCGGAGTTGCTCGTGGTGTCGGCGCAGACGCACGC41880GCMCTGMCGCGCMCTGGAGAGGATGGCTGCGTTCGCGTCCGGAGGCGCCGCACGCGA41940TCTGTCGCTCGCCGACATCGCCTTCACGCTGGCCGACGGTCGACGGCATATGCGCTACCG42000CTGGGCCTGCGTCGCAAGCAGCGTCGAGGAATTCGCCGCCTACTGCGGCGCCGGCGTCCC42060GTCGTCGCCATCGACGTCCACGGCGGTGTTCCAGGGCGACGCCMCGAGGCGCTGTCGCC42120GCATGTCGCCGATCGCGTCGGCGACGCCGACCTGATCTCGCAGGCGCGGCGCTTCGTGGC42180CGGCGGCCGGCTCGATTCCGCGCGATGGACGGGCCGCGGTTGCCGTCGCGTGCGTCTGCC42240CGTCTATCCGTTCGCACGCGMTCCTACTGGGTGGAGACGCCCGGCGAGGMCGCMTCC42300TTGGCCGCCGCTCGMGTGGCGGAGGCCGGGATCGGCCCGGTGTGGCGCTTCTCGCGTCG42360TCTCGMGGCAGCGAGCCGTTCCTGCGAGATCATCGCGTGCTCGGCGMTCCTGGCTGCC42420GGCCGTCGTCTACTGGGAGATGACGCGCTGGTCGGCCCGGCAGGCCGCGCCCGAGGCGCC42480CGCGACGGATCCGGTCGTACTGCGCGACGTGATCTGGCMCACCCGTTGTCGCTTGCCGC42540CGGGCCGCGTGTCGTTGAGGTGCGACTCGAGCGCGACGCCGCCGTGGCATTGTCGCCGCG42600CTTTCGCTTCGTGATCGAGAGTCGGCTTGCCGAGCCGGACGACGCTGCAGCGCGTCTGAC42660TCATTGCAGCGGMGCGTCGAGATCATGCGCACCGACGACGCCGGCGCGTCATGTCTGCT42720CGATCTGAGCGCGTTCCGGCGCCAGGCGGAGGMCTCGACGTGGCCGAGTGCTACCGCGC42780GTTTGCCGCGCTCGGCATCGMTTCGGGCCGAGCCATCGCGGCCTGAAMMCTCTATCG42840CTACCGCGATCMCTGCTCGCGCAGGTCGGCCGCTCGGGCGCCGCCGGGATCGACGACGG42900CTACCTGCTGTCGCCGACCATCACGGACGCCGCGTTGCAGGTCCACATCGGCMCGTGCT42960CGGCACCGGCACCGATGTGCTCCCGTTGCCGTATGCGATGGACAGCCTGACGATCGTGGA43020CGCACCGTCCGATCCGAGCTGGGTCTGGATCCGCCAGGGTCGCGMGGCGCGCGCGMCA43080TAGCCCGTCCAGCATGCTCCACGATATCGACGTGTTCGACGAGGAGGGCCGTCTACTGCT43140CGCGATGCGGGGCTGCCGCTCCGCGCCGGCACGGCGCCGCCGCATCGCGGCGCCGATACA43200CCCGCTCGTGCATGAGCGGGTCGACGGACCTGACTTGCGCTATCGCAGCGMTTCATCGG43260CGATGMTTTTTTCTGGACGGTCATCGGATCGCGGGCGTGAAGATCTTGCCGGCTGCCTG43320TTATCTCGAGATGGCACGTGMGTGAGCCAGCGCGTGGGCGCGCGCGGCGGTGTGCTGCG43380CGACATCTTCTGGCTCGCGCCCTACCAGGCGCCCGACCTCCCGGGCGGCGCGCGGCCGGC43440TCCCATCACCGTACAGATGGMGCGAGCGGCGAGCGGCATTGCCGCGTCGTCAGCACCGG43500CGCGGACGGTCGCGAGACCACGCACTTCGTGGGCCGCTGGGCGCMGAGGCCGTGCCCGA43560TGCGATCGGCGCGGTCGACGTCGACGCGATCCTGCGCCGTACCTCGACGCGTCTCGCGCG43620TGATGCGATGMCGCGCTCGTCGAGGCGACCTCGTCGTTTGGCGCACCGTTCCAGGTGAT43680GACATGGTTGCAGCACGGGGCCGATGAGGCGCTGGCCGCGTACAGGCTGCCGGCCGGGCA43740GAGCGGGCCCTATCACTGGCACCCTGGMTACTGAGTGCGGCGCTGGGTGCCGTCGAGAT43800ATGGATTGCGGCACGCGGCGAGACCGGCGCGCATCGCCTGCCGTATGGGATCGGTAGCAT43860GATCGATCACGCGCGGCCCACGGATGCCGGGTATATCCACGTGCGCCGCGTGTCGGGCGA43920ACGGGCAATGCTGGATCGCTACGATATCGACCTGCTCGACGCCGACGGGCGCATCACGGC43980GTCACTGCGCGGCTACTCCGTGCGGCCCTGGCTGTCCAGTGACCATCCGGATGCGGCCGA44040CGCCGGCTCGAGCCGTGCCGCGATTTCCGTGATGACTGGCACGCCCCGTTGGCGTGACGC44100GCCMCGCCGGCCCCGGCMCGGCGCGCGCGGACGACGCCGTCGATCGTCTATTTTTCGT44160ACCAGATCACGACACGGCGCTGCACGCGTGGCTGCGCGATCTCGACCCGGCGGCGCGCCT44220GTGTCCGCTGCCGTGCGGGGCGATCGTAGACGACGATGGCATGGCGCGCGTCGGTCGCGT44280CGAGGCAGCGTACCGTCAGGCTTTCGAGGCGATACGAGCCGTCGCGGATGGCGCCGGGAC44340CCGCGCCGTGCATCTGCTGATGCTCGTTCACGACGCGTGCCGCGACGMGCGATGCCGAG44400CCTGCMGCGCTGATCGCGACGGCGGCGCTGGAGTACGAGCGCATCGCCGCGAAMCCGT44460GTTCTATGCACCGGCAGGCGTCGCTGATCCGTCAGCCGCAGCCTATCGCGAGCGCTTGAG44520GCGCGMCTGCTGAGTCCCGMGCCGATGAACCGGMGTGCGCCTCGCGCTCTCGGGCGA44580ACGACAGACGCGTCGCGTCGCGGCGGCCGAGCTCCCCGCCCGCGCGGCGGCCGATGAGCG44640CCGCGACGGCGTGCTCGCGGCGCATCCTCTCGAGCCCGGCGATGTCGTCTGGCTGGTCGG44700CGGGATGGGCGGGATCGGGCAGCACGTGGCGCGCGATCTCGCGGCGCGCGGGCTGCGCGT44760CACGATCAGCGGGCGCAGCGTGGATCCGGCCGCATGGCGGGCGTTTCGTGAGACAGCGAG44820CACGGCGGGCGAGCGTCTCGACGCGCTTGCGGTCGATATCGCCGATGCGGACGACGTGM44880GCGCGCGGTGGCGAGCATCGTGGCGAMCACGGCCGTATCGACGCCGTGATCCACTCCGC44940GGGCGTGATCGGCGACGCGTACCTGCGTCACGGCCGCGCGGTCGATGCGGAGCCCGTGTT45000TGCCTCGMGCTGCGTGGCGGCGCGMTCTCGATGATGCGACGCGCGACCTGGACCTGM45060GTGTTTCGTGCTGTTCTCCTCGCTGTCCGCGTTGGGTAGCCCGGGGCAGGCTGCCTATGC45120GGTCGCCMCGCCTACTTGAATCAGTTGGCCCTGCAGCGCAATCGCGCCGCACTCGGGGG45180GCGGCGTCGCGGACGCAGCTTCGCGATCGGCTGGCCGCTCTGGCTCGACGGCGGCATGGC45240GATGGCGCCGGMCGCCGCGCACTGATGCGCGAGCGGACCGGACTCGATTCGCTGACGAC45300GGACCAGGGTCTCGCGATACTCGATGCGGTGCTGMCGCGTGCCGGATCGCGACGCCGGA45360AGACGGTGTGTTGCTGGTCGCGCCGGGTCCTGACCGCGAGCGCATCGTGCGAGCCCTGGA45420TGCCCGTTTCGTCGAGACGGMCCTGATGCCGGCGTCGCGGCAGGGCACGCGGCGGATAT45480CGACTTGCCGGMTCCACGCGGCTGCGCGCGGCTGTGCTCGACCTGCTGAAGGCGACGGT45540TGCGGCCCAGCAGACCATCGCCGCGGCCMGATTGACGACGGCGCACTGTTCCTCGATCT45600CGGTTACGACTCGATCAGCCTGATCGGTCTCGCGCGTGATCTCGGCGCGCGGGTCGGGTT45660GACCTTGCAGCCMCGCTGTTTTTCGAGTATCCGGTGCTCGTCGATGTCGCGGATCATCT45720TGTCGCGTCTCACCGCGACAGGCTGGCTGCGCATCTCGGGGGCCGGCCGGCTTCCGCACG45780TGCCGCTGTGGGTTCGMCGAGCCTGCGTCGGTMGCGATTCGGCCGATGMTCGCGTCG45840CGCGCAGGCTGCCGGCGTGGCATCCGGCGAATCCGCGCGGCMGCGGATCCGGTCACGAC45900ACGCGACCGCGTCGCCGTGATCGGCATGAGCTGTCGCTTCCCCGGCTCGGACAGCCCCGA45960CGCATTCTGGCGCMCCTGACGGAGMCCTCGACCTCGTCGGTCGAGCGACCGGCCCGCG46020CGCATCGTTGCTCAGCAGCGTGACGACGCCGTCCGMGGCGGTTTCGTCGATGACATCGA46080GCGCTTCGATCCGCMTTCTTCGGCTTGACGCCGATGGAGGCGGAGTTCATGGATCCGCA46140GTTCCGTCTGTTCCTGCMTGCGCGTGGCGCGCCGTCGAGGACGCCGGCTACCGGATCGG46200CGATCTGGCGMGCGGCGAGTCGGCGTCTTCGTCGGTGTGACGACGAGCGACTACCGCGA46260TCTATGGCTGAGCCGGATCGGCGCCGAGTCGGCGACTCACCTCGGCCTCGCGCACTTCAT46320GATCGCGMCCGCGCGTCGTACCAATTCGACCTGCGCGGCCCGAGCGAGGTGATTGACAC46380TGCCTGCTCGAGTTCGCTCGTTGCGATTCATCGTGCCTGCGAGAGCCTGGCCTGCGGCM46440TTGCGAGATCGCGATCGTCGGCGGTGTCMCGTGATTGCGAATCCCGCCGTCACCGAGGC46500GGCGGTGGCCGCCGGCATGCTGAGCCCGGACGGACGCTGCAAGACTTTCGACAGCAGCGC46560CMTGGCTTCGGCCGAGGCGMGGCGTGGGGGTCGTCGTACTGMGCGCATGGCCGACGC46620GCTCGCGGACGGCGACCATCTGGACGCCGTCGTCGTCGCGAGCGGCGAGAATCACGGCGG46680ACGTTCCAGCTCGCCATCCGCTCCGMTCCCGATGCGCAGCGCCMCTCATCGTCGATAT46740TTATCGACGTTCAGGCATCGATCCCGCCAGCGTCGGCTACGTCGAGGCCCATGGTACCGG46800CACCGCGCTCGGCGACCCGATCGAGATCMGGCGCTGGTGCGCGCATTCGCCGAGCTTGC46860CGACGGCCGGCCCGCCGAGGCGCCACATTGCGCGATTGGTTCGGTCMGGCAMCATCGG46920CCATCTCGAGGCCGCCGCCGGGATTTCCGCGTTCATCAMACGGTGTTGATGCTCMGCA46980CGGCMGATTCCCGGTMTCCGCACTTGMGCAGCCGMCCCCTATCTCGAGCTGGATGA47040CAGCCCGTTCACTTTGGCGAGCGAMCGTGCGACTGGCCCGCGTCGCATCGACGTGCCGG47100CGTCAGCAGCTTCGGACTCGGCGGCTCCMCGCGCATATCGTGCTGGACGCCGCCGATGC47160GTATCCGGCCGCCGCTCCAGGCMCGTGCCGGAGCCCGCGTCGGGTCCAGTCGCGATCGG47220GTTGTCCGCCCGGCGGCCGGCGGAACTCGACGCGATCGCCGTCGCGTTGCTGGACTTTCT47280GTCCGCGCCGACTGCCGCCATGCCGTCGCTGCGGGATCTCGCGTTCACGCTTGATCGCGG47340GCGCGAGACCCATCCGCACCGGCTCGCGTTCCTCGCCACCGATATCGACGACGTGCGACG47400AMGTTGGCGCGCGTCMCGCGGGCGACGATGCGGCGACGCTGGCCGCGCATGGCGTCGT47460CGTCAGCCGCGCCGGGGACGATAGCGCCACGCGTCACGCGCACCTGCTCGCCGGGGCGTA47520TCACGACCTCGCCGAGCGCGCGCCGGMGTGCTGCGCGATCTTGGGGCGTGGGTGCGCGG47580CGAGATTGACACCTGGCGCGGACCGCTGGCCGCGCGCGGTCGGCGGGTCAGCCTGCCGAC47640CTATCCGTTCGGAGGCMGTCGTATTGGTTACCCGMGCGCCTCGGTCAGTCAGCGCGGC47700GTCATCGGTACTGCATCCGCTGCTCCATCGCGAGGTGTCCGCCGAGGCGGGCATCCGCCG47760CTTTGCCTCGATATTCAGCGGCGAGGAGTGTTTCCTCGCGGATCATCGGGTCCAGGGGCA47820GCGGCTGCTGCCCGGCGTCGCGAGCCTCGAGATGGCACTGGCGGCCGCCTGCCTTGCAGC47880CGGAGMACAGGCGACAGCGGTGCCACGCTACGCMCGTTGTCTGGTCGCGGCCTGTCAT47940CTGGGGCGCGGCGCATTCGGATGTCGCGCTGAGCCTGTCCGCGGGGGCCGTGCCGCGCGC48000GTGGCGCTACCGGCTCGGTGTCGGCGGTGACGATACGTACGGCGAGGGCGAGGTCGCGCT48060GACGGCCATGCCCCCCGTGCGCACGCTCGACCTCGMCGGATGCGTGCCGAGCGGGACGG48120CTCGCGGGTGTCGGCCGAGGCGTGCTACCGTCGCTATCGGGAGGTCGGCATCGMTACGG48180GCCCAGCCATCGCGGGCTCGMCGGCTTTATCTCGGCAGTGGCGACGTGCTCGCCGMAT48240CGCGCTGCCAGCCGGCAGCGTGGACGCCGCCGGGGCATTCCGGCTCCATCCCGGATTGCT48300CGATGCCGCCTGGCAGGCGGTCATCGGTCTCACGATCGATCGCGGTCAGCACGAGGCGAG48360CCTGCCGTTCGCGCTCGACGAGATACGCATCGTGCAGCCGCCGCGAGCGTCGCGGCTGTG48420GGCCTGGATCCGCCCGGGCGACGATGGCGCGGCGAGCGTCGACATGGATATAGCGCTGCC48480CGATGGCCGCATCGCGATCGAGCTTCGCGGGCTCCACACGCGCCAGCAGGTCGCGCCGGC48540CGCTTCAGCGCCTCGGGATGAGGGCGCCGTTACCGGGGATGGCGGCCTGCTGCCCGCCGG48600CGAGGTCACGCTGAGCCCCGTCTGGGMTCACTGCCTGCGCAGGACGGGGCGGTGCCGAG48660CGGGCCCCGCGAGCTCTGGGTGTTCGGCTCGAACGAGGCCGTGCGCGCCGCATTGGAGGG48720GACGCGCACGGTCATCTGTTTCGACAGCCTCGATGGCACGGACGACGCCTTCCGCCGAAC48780CTTGMGGCGTATGCCGAGACGGCMGGGCCGAGGCTCCGCATCTGCTCTGGATCAGCGG48840CGGCGGTGAGMCCCAGACCTCGACCCCGACGCACTGATTGCGCGCCAGTCACGCGGCAT48900CCTGCCCTTGTTCCGATTGATCMTGCGCTGATCGAGGCCGGTCTCGATAGCGTGCCGCT48960TGCGCTGACGGTGGTTACGCGCAGGCTGCACGCGCTCGGCGATGAMCCGACMCGACAT49020CTCCGATGCCGGCGTCGTCGGCCTCGTTGGCACGGTGGCGCACGMTATCCGCATTGGAC49080GGTACGGCACTGGGACGTCGACGCGCCGGTCGACGACGGCCGCTTCTTCCGTCGCCTGGA49140CACGCATCTCATCACGACGGCCGGCGCCGACGGCGACGTGCGCGTGCATCGCGGCGGCCG49200CTGGCACCGCCAGCGTCTCGCGCGCGTCGTCGATGAGGGGCGTGCCGATCCGGTATATCG49260CGMGGCGGCGTCTATCTCGTGATCGGCGGCGCGGGCGGGTTGGGCAGGACGTGGTCGCG49320CCACATGATCGAGCGATTCGGCGCGCAGATCGTCTGGCTCGGGCGACGCGCACCGGATGA49380CGAGATCCGCGCGCATCTCGCCGCGCTCTCCGCGCTCGGGCGCGGCGGCATCACCTATCT49440GCMGCCGACGCGACCGACGCCGTCGCGCTGAMCGCGCCCGCGCGGCGATCATGGAGCA49500ATTCGGCGCGCTCCACGGCATCGTGCATGCGCCGATCGTCCTGGCGGATCGCTCGCTGGC49560GAGGATGGACGAGACAGCATTCCGTCTGAGCTACGACGCCAAGCTGMGACCTGCGCGM49620CCTGTATCCGCTGTTCGCCCGGGAGCCGCTCGACTTCGTGCTGTTCTTCTCGTCGCTGM49680CGCGTTCTCGCGCTCGGCCGGGCAGGGCMCTACGTGGCCGGCTGCACGTTCACCGACGC49740TTACGCGCGCMGCTGGACACCCGGCTGGCATGCGACGTCAAGGTMTCAACTGGGGCTA49800TTGGGGCGACGTCGGCATCGTGGCCGGCGCGCGTCACCGCGMCGGGCACTTGCCGCGGG49860GCTCGATTCGATCAGCGCCGAGGACGGTCTGCGCGCGATCGACGCATTGCTCGCGCGCCC49920CGGTTCGCAGGTCGCATACGTCAGGACGGCCCGGCCCATCGCAGCGCGGCACTACGACTC49980GACCACGTTGGTATGCCGCTATCCGCGCACGATCCCGGCGGTCGAGGCGGCGCGGTTCGC50040GGCACGCCCGGCCGCCGATCTCGACGCGGTGAGCCGCGAGACACTCGGTTTCAGGCAGCG50100CGTGGGAGCGTTGGCCGAGCGGCTGATGCTGGCGACGCTGCAGCAGGTCGCGCTCGCGCC50160AGCGAGCGGCGCAMCGCCCCCGCCGGCACGTTCGACGAGCGGCTGCGCGCCGATGCGCC50220GCCGCATGTCGACGCCTACGCGGCGTGGCTCGCACAGAGTCATCGCCTGCTCGTCGCGGC50280GGGTCATCTGTCCGTCGACGGCGAGTTCTTGTCGTGTCGCGCGCCGGCCTGTTCGCTCGA50340TGCGCTGTGGCAGGACTGGCGCGGGCTTGCCGAGGAGTACGCGCMCAGCCCGGCTATGT50400CGCGCAGTTGGMTTGCTCGAGTCGTGTTTGCGCGCACTCGGCGAGATCCTGCGCGGCGA50460CGTCGCGGCGACCGACGTGATGTTCCCGGGCGGCGGCATCTCGCGTGTCGMGGCATTTA50520CCGGMTMCGCGGTATCGGACCACTTCMTCACGTGCAGAGCGAGGCGATCGCCGCGTA50580TGTCGAGGCGCGGGTGCGGATGCAGCCGGACGCGCAGCTGCGCCTGCTGGAMTCGGCGC50640CGGCACGGGCGGCACGACCGACGGCCTGCTGCGCCGACTCGAGCCGTGGCGCGCGCATAT50700CGCCGAGTACTGCTACACCGATGTGTCGMGGCCTTCCTGATTCACGGCGAGACGCGCTA50760CGGCGAGCGCTACCCATTCCTGTCGACGMACGGTTCGACGTCGAGMGGCGCCGGCCGG50820GCAGGGGATCGCCGAGMCCATTACGACGTGGTGCTGGCAACCMCGTGCTGCACGCGAC50880CGCGTCGATGCGCGACACGATGCGGCATCTGAAGGCGATCCTGCGCCCGTCGGGCATTGC50940GGTCGTCMCGMGTCAGCGAGGTGMCGTGCTTGGACACGTCACGTTCGGCCTGCTGCC51000CGGCTGGTGGCGGAGCGTCGATCCCGMCTGCGCATCCCGGGTGGCCCCGCGCTGTCGCC51060CAGCGCATGGGCCACGCTCGGCGCGGCTGAAGGGCTGCGTCCACTGGGTTTCCCGGCGCG51120CGMCAGCATGCGCTCGGGCAGCAGATCATCGTGTTCGMAGCGACGGCGTGGTGCGCCG51180GGCGCTGACGGCCGACGCGTCGCCGGCGACGGGMGCGCGCTCACCAGGCCCGATGTGCG51240GGCGCGGTCCACCCGCACGGGCGATTCGCACGGTACMCGGCGACCGTGTCGGAGGAGAT51300GCGCTTCMCGGCGCGCTTGCGTTTGTACAGCGGCTCGTCGCCGATACGCTGMGATCGA51360TCTGGATGMTTGCCTCCCGACGAGCACTTCCAGTCGATCGGCCTCGACTCGATCCTCGT51420CGTCCAGCTGACGMCCAGCTGCGCCGCTATTTCGACGGCATCACMGTCCGCTGCTGTT51480CMGGTCTCGACGGTACGCGCGCTGACCGAACATCTGTGGCTCGCCGMCGGGAGCGGCT51540GCTCGAGCACGCGCATGGTGCCGTGCCGGGAGCGGATGMGCCTCGGCCGTCGCCGCTGT51600CGGCGATCGCGGTACCGGCGCCGTGCATGGCATACGTCCGGCTGTTCCGGACGATGGCAT51660CGCGGATTCCGACGTCGCGATCATCGGCATGAGCGGACGGTTTCCGGGCGCGCCCGATCT51720CGCGGCGTTCTGGCGGATTCTGCGAGATGGCGTGMCTGCATCAGCGMGTGCCGCCGGC51780GCGCTGGCGCTGGCAGGACTACGACGACCCCGMCTCAGCGAMCGGACCGGATCTACAC51840CCGCTGGGGCGGCTTCATCGAGGACATCGACCGTTTCGATCCACTGTTCTTCCAGATCTC51900GCCGMGGACGCGGMAAMTGGATCCGCAGGMCGCCTGTTCCTTGAGGMGCCTATCG51960CGCGATCGAGGATGCCGGTTATACGCCGTGCGGGCTCAGCGMGCCGGCAGCGTCGGCGT52020GTTCGTTGGCGCGATGMCGGGACCTACMCGCGCAGCCGAACTTCTGGTCGATCGCCM52080CCGGGTTTCATATTGCTTCGATTTGTCCGGGCCGAGCATGGCCATCGATACCGCCTGTTC52140CTCGTCTCTGACGGCGCTGCACACAGCGCTCGAMGTCTGCATTGCGGCTCGTGCGACGC52200CGCGATTGTCGGCGGGGTCAGTCTGATTGTCGATCCGGTCCAGTATCMGGGCTGTGCGC52260MTGACGATGCTGTCGCCGGGCMTCGCTGCAAGGCATTCGGCGCCGATGCGGACGGTTT52320CGTCGACGGCGAGGGCGTCGGGGTGTTCGTGCTCMGCCGCTGCGCMGGCGCTCGCCGA52380CGGCGATGCGATCCACGGCGTGCTGMGGGCAGCGCGATCAATGCCGGCGGCMGACTCA52440CGGCTACACCGTGCCCMTCCCGCACGGCAGTCGGAMCCCTGCGCCGTGCGCTGGAGCG52500CGCTCGCGTGGCGCCGGCCGAGATCGGCTACATCGAGGCGCACGGCACCGGCACGGCGCT52560CGGCGATCCGATCGAGATCGCGGCGCTCACCGATGTGTTCGMGGATCGACGGACATCGA52620GGACGGCACGGGCGCCGCCTGTCCGATCGGCTCGGTGMGTCGMCATCGGCCATTTGGA52680MGCGCGGCCGGCGTCGCAGGTGTCGCGMGGTATTGCTACAGCTCCGGCACCGCCAGTT52740GGTCGCATCCTTGCATGCGGCCGAGGGTMTCCGGAGATCGATTTCAGCCGCACGCCGTT52800CCGAGTCCAGCAGMCCTGGCCGACTGGACGCCCCGGGTGACGCGCGGAGGTTCCCCGGC52860GCGCATCGCGGGCGTGTCGTCGTTTGGCGCGGGAGGGGCGAATGCGCACGTGATCGTCGC52920CGAGCCGCCCATGCGCCGCGACGAGCGGACGCCCATCGCGCMGGCCCCGTGATCCTTGT52980GCTCTCCGCTCGGAGCGCGGATCGGCTACGCCGGAMGTGGCTGACCTCGTGCGTCATCT53040CGAGACGGACGGGGCCGGCGTAGCGCTCGCCGATCTGGCGTTCACGCTGCAMCCGGCCG53100AGAGCCGATGCCTTGGCGCGTCGCGTGGCTCGCCGATAGCGTCGAGGGGGCGCTCGACCA53160GCTGAGGGACTGGCTCGCCCGAGAAGACGCGACCGGCACCGATGCGAGCGTGTTCGGCGC53220GGCGGCTAGACAGGCCGATGCGGTGGCGCGACGCATGCTCGACGATCGCGATCTGGCGTC53280GCTCGCGCGTATCTGGACCGACGGCGCACGGGTCGACTGGGCGCGTCTGTGTGATGGTGA53340GGTCGCTCGCCCGCGCCGACTCCATCTGCCGACCTATCCGTTCGCCGGCGGTTCGTATTG53400GCGCGCCCCGACGGCACGCGACMGGAGCGCGTCGCGCACGCCGGCGACGGCACTGGCCG53460CGCGGACGAGGAGCACACCGATGCCTTCMCGMGCATTGTATGCATCGGTCATCGACCA53520TCTGATCMCCAGGAGCTGGACCCGCAGGCTGCACTGCGGGTGGTCCAGTCTGGACGCTG53580MCCTGCTTGCGCGACGGCGCTTCGGCGCCAGTCCGCGCAGCGCCACGGAMTCTCATGG53640CTMTTATCATCTCGACATCTTTGGGCAGGTCGCACGCMCCGTCTGCGGCGCGACGCAG53700CGTTGMCCTTCTGCGCCAGTACCGGCGTTCGGGTTGCATCGGCGGMCGGGAGAGGTTC53760ACTGGTTGCACTCGCAACCACCGGACGCGGCAGGCCGCGAGMCGGCGGCGTACGCGCGG53820TGCTGTATTGCGGGTTGACCGGGCGCGAGTTTTTTCTCGCGGATCATTGCGTCGACGGGG53880TGCATGTGCTGCCCGCCGCCGCCTATTTCGAGTTCGTGCGCGCGGCGGTCCGACAGGCGG53940GCTTGGCCCATTCCGACCGTTTCGTGATGAAGCMGTCAGTCTCGMCGGCCGCTCATGC54000TCGACCGTGACGGCGACGCCGACGATTGCCGTCTGCTCGTCGTGGMCTGCTGGCGGATG54060CGCGCGGCCGGATCGATTTCACGCTGGCATCGACGGCGTGCACGCTCGCCGAMTCACGC54120CGCGCCTCGACGCGACCCCGCACGCGGCGCTGGCCGCCGATGAGAMTTGGTCGTCCACT54180[1001]GTCGCGGCGTCTACCTGTCCGCGCCGGAGTCCGAGCCGGTCGCCGTCGGCGACGTGACGC54240ACGAGATTGCGTCCAGCACGACCGTCTTCGACGCCGMCAAGTCTATCGCTTCCTGGCGG54300CGCGCGGTTTCGMTACGGTCCGCGGCAGCGCGCGCTGCAACGCGCCACGCTGCATTCGC54360CGGMTGCCTGTACGCGGATCTCGCGCTGCCGGTTCCGCTTGTGTCGAGTCTGCCGACCA54420TGATGTTGCATCCGAGTCTGCTCGACGGCGCGTTACAGGCCGCCGCCGGCTTCTATCTGG54480CCGATGCGGCCGATATCGAGCCGGGGCTGCCGTTCGCCATCGACGAGATCGCCGTATGGA54540GGGTTGGCGAGCCGAGCATGCGGGCGCGCGTGACGCGCCGCACGACAGAGGTCGGTGCGG54600TCACGCGCCTCGACATTGAGTTATACGATGCGGACGGGCGGCTCTGCGCGGCCTTGMGG54660GGCTGACTTCTCGCGTCGGGCGCATTCGAGCCCGGCACGGCAGCGGGTTGCTGCTTGCGC54720GTCCCGAGTGGCGTGCCGAGCCATTCGTGGTCGGAGCGCGAGCCGCACGGCMCCGGCGC54780CGTGCCGGATTTTGCTGGTCGGTGATCTTGGCMGGTCGGCGAGGTCGATGCGCTCGCCG54840CGTCGCTACGGACACGCTGGCCCGCCGAGCTGGCGGGTCCGGAGATCGTCCGCATCGCGT54900TTGATCCGGTCTCGGCGCMGACGACCCGATCGAMGCAGCTTGCGCCTGMGCACTGCT54960TGCAGGACTGGTCCGCCATCCCCTGTCGAGMTCCGMCTGTGGCTGGTGGCMTGGACG55020ACGCCTCGCCACTCGCACGCAGCATGCATGCGATGCTGMATGCATCGCAGCCGAGGATC55080CTCGCCGTGCCACGCGGCTCGTGTCGTGTACCGCACGCGCCGACGTGMTGAGATCGCGG55140GCGCGCTCGTCGACGAGGCGCGTGCGGACGACGATGTCGACTGCGTGCGCTACGCGGGCG55200TCCGTGAGGTGMGCGCTGGGTTTCAGTCGCCGCCGAGGCAMCGCACCAATGCATGGGT55260GGCGTGATGGCGGCCGCTATTTGATCAGCGGCGGCACGGGCCATCTTGGCCTTGCATTCG55320CGCAGGACATCGCCGCGTCGGTTACCGGGGCGTGTATTTTCCTGCTCGGCCGACACGGGC55380CCGACGCGCTCGATGCGCGGCAGCAGACGCGCCTGCGCGAAATCGCCGCCGCCGGTGCTC55440GGGTGATGCACTGCCGCGCCGACGTCGCCGACGCCGAGGCGGTGGCCGAGACCATCGCGC55500GMTCGAGCGTGAGCATGGGCCGTTGCACGGCGTATTGCACGCGGCGGGCGTGGCCGACG55560ACGGCTTGCTCGTGMCMGACCGACGATACGTGGCGACGCGTGATGCGACCGAMGTGC55620TCGGCGCCGCGMCCTCGATCGCGCGACGCGGCACATGCCGCTCGACTGTTTCGTGCTGT55680TTTCGTCCGGCAGCGCGGTGTTCGGCMCGTCGGACAGAGTGACTACGCGGCGGCGMTG55740CCTATCTGGCCGGCTACGCGCGATGGCGGCMGCCGAGGTCGGGATCGGCGCCGCGCATG55800GTCGCTCGCTCTGTCTCCACTGGCCGCTCTGGGATGGGGGCATGGCTGCCGGGATCGCCG55860ATCCGCAACGCCTGTATCACGCCACGGGCATGCGCCCCATGGACGCCGTGCTCGGCATCG55920AGGCGTTTCGCCGCGCGCTCGCCACGCCATGCACCGMTTGCTCGTGGCGGCGGGCGATC55980TCGACCGGCTCMGCGGTACCTGACCGGCGTCGCCGATACGGATGCCGCCGGGGCCGACG56040MGCCGCAGCCCCACCCGCGCCGGATCTGTCGCMGTCGATTCGGAGGCGTTGCACGGCG56100CGGCCGTGCGTCTGCTGCGGCGGGTTGTCGGTGGCGTGTTGAMCTCCAGGTCGAGCAGA56160TGCGGGCCGACCAGCCGCTCGATCMTATGGCATCGACTCGGTCGACATGATGMGGCGA56220CGCGCGAACTCGMGAGACGTTCGGMCGTTGCCGMGACCCTGTTCTTCCAGTATCCAT56280CCATTMCGAGCTGGCCGGGCATTTCGCCGAGCACCACGCCGAGGCGCTGGCCGCGACAG56340GGACGCCCGTCGCGCCTGCATCGGGCCCGCGCGCGACGCAGGTCCCCATGACGCAGCCGA56400TCTCCCATCCGGCGGAGATGCCGCGCCCGACGCCAGGCGTCGTCGACGCGTCCTGCGTGT56460[1039]CGGGCGATGCCGCGCGCGCGGATATCGCGATCATCGGGCTGTCCGGCCGCTATCCGCAAG56520[1040]CGGCCGATGTCGTCGAGTTCTGGGACMTCTCGCGGMGGCGTCGACGCGATTCGCGAGA56580TTCCACCGGAACGCTGGGACTGGCGCTCGTTTTATCGCGAAGACGGCAGCCGTCGCCCGG56640GCACGCACGCGAGCCGGTGGGGCGGCTTCATCGACGGCGTCGACGMTTTGATCCGCTTT56700TCTTCAGCATTTCGCCGAGCGAGGCGGAMTCATGGACCCGCMGMCGCCTGTTCTTGC56760AGCATGCGTGGGCCGCGCTGGMGATGCCGGTTATTCGCGTGGCCGCCTCGCGCGTGCGC56820GACGCGAGCGCGAGGCGGCGGGCGACGAGGCCCGCATGGACGAGATCGGGGTCTACGTCG56880GCGTGATGTACAGCGAATACCTCCAGTGTGCCGACMCGATCCGGMCGGCTTGTGGGCA56940GCAGCTACGCGAGTATCGCCMTCGCGTGTCCTATGCGCTCGATCTGCGCGGCCCGAGTC57000TCGCGATCGACACGATGTGCTCCAGCTCGCTGACCTGCCTGCATCTGGCTTGCCGCGAGC57060TGGCCTCGGGCGGCATCGGCATGGCGCTCGTCGGCGGTGTCMCCTTMCCTGCATCCGA57120ACAMTATCATCTGCTGAGCCAGGGTCGCTTCGTGTCGCCGCGAGGCCGCTGTCACAGCT57180TCGGCGCGGATGGCGACGGTTACGTGCCGTCGGAGGGCGTCGGCGTGGTGTGTGTGMGC57240GGCTCGCCGACGCGGAGCGCGATGGCGATCCGATCTACGCAGTGATCCGCGGCAGCGCGA57300TCMTCATGGCGGCMGAGCCMGGTTATAGCGTGCCGCAGCCGCGACGTCAGCAGGACG57360TGATTCGCCAGGCGCTGACGCMGCACMGTCMCGCGCGCTCGGTCAGCTACGTCGAGG57420CGCATGGGACGGGCACCGCGCTCGGGGATCCGATCGAGATCGCCGGCATCGCCGGCGCGT57480TCTCACGCGATACGGCCGATACGCAGTTCTGCGCGATCGGCTCCGTCMGTCGAGCGTGG57540GCCATTGCGAGGCGGCTGCCGGCATGGCTGCGCTMCCMGGTGATCGGCCAGATGCGCG57600CCGGCGAACTGTTCCCGTCACTGCACGCGCMCGGTTGMTCCGMTATCGATTTCGCGG57660CGAGCCCGTTCGTGGTGGTACGCGATCGGCAGCCATGGMGGGGGCGACGGGCTCCCGGA57720TCGCAGCCGTTTCATCGTTCGGCGCAGGCGGCTCGMCGCGCATTTCGTGCTGGGCGATT57780ACGCGCGGCCGGCCGATGACGCGATCGATGGCGAGGACGGAGGTATTTGCCTCGTGCCGC57840TCTCGGCCCGCACGCAGGAGCGATTGGCGGAGCAGGCACGCGCGCTGGCCGCCTATCTGT57900CCGACTGCGCGCGGCCGGGGGCGGGCGGGCGCCACTGGCGGCATCCGCCCGCCGGGCTCG57960CGMCATCGCCTATACGTTGCAGACGGGGCGCGAGCCGTGGGAGGCGCGCGTCGCATTCA58020TTGCMCATCGCGCGACGMCTCCTCGCGCTGCTGMCGGCTATCTCGACGGCCGGCCCG58080ATGCGCACAGCGTGTTTTCGGGCACGCGCGCGMCGGTMCCGGCTGCTGCCGCTGTTGG58140GCATGGATCAGGACAGCCATGCGCTCATCGATACCTGGTTCGCGMGCGCMGTATGCGG58200MCTTCTGACCGTGTGGGTCGAGGGCGTCGATTTCGACTGGGAGCGCTTGTATGCCAGCG58260ACGGTGCAGGCGGGCGCCGACCGCGACGTGTCMTCTGCCGACCTATCCGTTCGCGCGCG58320MCGCTACTGGCTGCCGACGCCGGACGTCGAGGCGCCGCGTGCCGAGCCGMCCGCMGG58380CCATGACGMCCTGACGACGACACCGCGAGTCGGCTACCGTGTGGCAGTGTGGGAGCGCC58440TGCCGATGGGCGCGGAGGCGTCCCACGCCGTGACCGACGTCTTGCTGTGCGGTTGGGATG58500CGGCCGAGGCGCAGCGGTTCGCGGCCCACTGGCCGGCGCATCGCTGCCGGATCGTCGGAT58560CCGCTGCGGATACCGATGCCATCGACGGCGCGTTCACGCAGCTCGCGCTGGCGGTCTTCT58620CGGACCTGCAGGCGCGGCTCGCGACCGCGGCCGGCGCCMCGTACGCGCGCMCTGCTGG58680TGCCGCCCGGCGACCGCGGCCGGCTTCCGAGCGCGCTGGAGGCGATGCTGCGCAGCCTGA58740TGCTCGAACATCCGACTTTCGCCGGCCMCTCGTGCMTGCGACGATGTGTCTTCGGACG58800CAGCATTGCGCGAGGCGGCCGGATTGGCCGCGCACCACCCGCGCGCGACGCTGGTCCGCT58860[1079]ACCACGAGGGGGCGCTTTCGCAGCGCGTGTGGCGCGAGGCGGACGGCGACGAGCCCGGCG58920AGTCGCCGTGGCGCGAAGGGGGCGTCTACCTGATCACGGGCGGCGGTGGCGCGCTGGGCA58980[1081]CCCTCCTGGCGCGGCAACTCGCGGGCGMCTGTCGTCCGCGACCCTGATCCTGACAGGCC59040GTCGCGCAGCCTCCGAGCTGACAGGCGCGCCGGTCGGCAGTTTGCTCGACACGCTGCGCG59100CGCMGGCATTGCGATCGMTATCGTGCGGCCGACATCGCCGATCTCGCGGCCTGTGAGC59160AGCTCCTGCCCGACATCGTGCGCGAGCACGGGGCATTGMCGGCGTGTTCCATGCCGCCG59220GGGTGAGCCGAGATGGCGCCGCGTTGCGCAAGACGGCGCAGACCTTCCGCGMGTGCTTG59280CGCCAMGGTGGCGGGCACCGTGAATCTCGACTGCGCGACGCGCCATCTGCCACTCGATT59340TCCTTGTGCTGTACTCGTCGCTTTCGGGAGCGATGGGCMCGCGGGGCAGTGCGACTATG59400CMCGGCCMCGCTTTCCTGGACCGCTACGCCGCTCACCGCGCGGTACTTGCCGCGACGG59460GCGCGTGTCACGGCCGCACCGTATCGATCGGCTGGCCCTGGTGGGMGGCGAGGGCATGG59520CTCCGGACGCGCATGCGCCGGGCGTCTTCGTGTCGCCGCGAGGCCGCTGTCACAGCTTCG59580GCGCGGATGGCGACGGTTACGTGCCGTCGGAGGGCGTCGGCGTGGTGTGTGTGMGCGGC59640TCGCCGACGCGGAGCGCGATGGCGATCCGATCTACGCAGTGATCCGCGGCAGCGCGATCA59700ATCATGGCGGCMGAGCCMGGTTATAGCGTGCCGCAGCCGCGACGTCAGCAGGACGTGA59760TTCGCCAGGCGCTGACGCMGCACMGTCAACGCGCGCTCGGTCAGCTACGTCGAGGCGC59820ATGGGACGGGCACCGCGCTCGGGGATCCGATCGAGATCGCCGGCATCGCCGGCGCGTTCT59880CACGCGATACGGCCGATACGCAGTTCTGCGCGATCGGCTCCGTCMGTCGAGCGTGGGCC59940ATTGCGAGGCGGCTGCCGGCATGGCTGCGCTAACCMGGTGATCGGCCAGATGCGCGCCG60000GCGMCTGTTCCCGTCACTGCACGCGCMCGGTTGMTCCGMTATCGATTTCGCGGCGA60060GCCCGTTCGTGGTGGTACGCGATCGGCAGCCATGGMGGGGGCGACGGGCTCCCGGATCG60120CAGCCGTTTCATCGTTCGGCGCAGGCGGCTCGMCGCGCATTTCGTGCTGGGCGATTACG60180CGCGGCCGGCCGATGACGCGATCGATGGCGAGGACGGAGGTATTTGCCTCGTGCCGCTCT60240CGGCCCGCACGCAGGAGCGATTGGCGGAGCAGGCACGCGCGCTGGCCGCCTATCTGTCCG60300ACTGCGCGCGGCCGGGGGCGGGCGGGCGCCACTGGCGGCATCCGCCCGCCGGGCTCGCGA60360ACATCGCCTATACGTTGCAGACGGGGCGCGAGCCGTGGGAGGCGCGCGTCGCATTCATTG60420CMCATCGCGCGACGAACTCCTCGCGCTGCTGMCGGCTATCTCGACGGCCGGCCCGATG60480CGCACAGCGTGTTTTCGGGCACGCGCGCGAACGGTMCCGGCTGCTGCCGCTGTTGGGCA60540TGGATCAGGACAGCCATGCGCTCATCGATACCTGGTTCGCGMGCGCMGTATGCGGAAC60600TTCTGACCGTGTGGGTCGAGGGCGTCGATTTCGACTGGGAGCGCTTGTATGCCAGCGACG60660GTGCAGGCGGGCGCCGACCGCGACGTGTCAATCTGCCGACCTATCCGTTCGCGCGCGAAC60720GCTACTGGCTGCCGACGCCGGACGTCGAGGCGCCGCGTGCCGAGCCGMCCGCMGGCCA60780TGACGMCCTGACGACGACACCGCGAGTCGGCTACCGTGTGGCAGTGTGGGAGCGCCTGC60840CGATGGGCGCGGAGGCGTCCCACGCCGTGACCGACGTCTTGCTGTGCGGTTGGGATGCGG60900CCGAGGCGCAGCGGTTCGCGGCCCACTGGCCGGCGCATCGCTGCCGGATCGTCGGATCCG60960CTGCGGATACCGATGCCATCGACGGCGCGTTCACGCAGCTCGCGCTGGCGGTCTTCTCGG61020ACCTGCAGGCGCGGCTCGCGACCGCGGCCGGCGCCMCGTACGCGCGCMCTGCTGGTGC61080CGCCCGGCGACCGCGGCCGGCTTCCGAGCGCGCTGGAGGCGATGCTGCGCAGCCTGATGC61140TCGMCATCCGACTTTCGCCGGCCMCTCGTGCMTGCGACGATGTGTCTTCGGACGCAG61200[1118]CATTGCGCGAGGCGGCCGGATTGGCCGCGCACCACCCGCGCGCGACGCTGGTCCGCTACC61260ACGAGGGGGCGCTTTCGCAGCGCGTGTGGCGCGAGGCGGACGGCGACGAGCCCGGCGAGT61320CGCCGTGGCGCGMGGGGGCGTCTACCTGATCACGGGCGGCGGTGGCGCGCTGGGCACCC61380TCCTGGCGCGGCMCTCGCGGGCGMCTGTCGTCCGCGACCCTGATCCTGACAGGCCGTC61440GCGCAGCCTCCGAGCTGACAGGCGCGCCGGTCGGCAGTTTGCTCGACACGCTGCGCGCGC61500MGGCATTGCGATCGAATATCGTGCGGCCGACATCGCCGATCTCGCGGCCTGTGAGCAGC61560TCGTGGCCGACATCGTGCGCGAGCACGGGGCATTGMCGGCGTGTTCCATGCCGCCGGGG61620TGAGCCGAGATGGCGCCGCGTTGCGCMGACGGCGCAGACCTTCCGCGMGTGCTTGCGC61680CAMGGTGGCGGGCACCGTGMTCTCGACTGCGCGACGCGCCATCTGCCACTCGATTTCC61740TTGTGCTGTACTCGTCGCTTTCGGGAGCGATGGGCMCGCGGGGCAGTGCGACTATGCM61800CGGCCMCGCTTTCCTGGACCGCTACGCCGCTCACCGCGCGGTACTTGCCGCGACGGGCG61860CGTGTCACGGCCGCACCGTATCGATCGGCTGGCCCTGGTGGGMGGCGAGGGCATGGCTC61920CGGACGCGCATGCGGCGCGCGTGCTGCGTGAGACGGGCATGCTGCCGCTCGGCGCCGACG61980ATGGCATGCGCGCGTTGAMCGCGTACTCGTGCATGGAGGCGMCACGTGCTCGTCGTCC62040ATGGCGACGCCGCACGCATGCGCGCGGCCGCCGAGCMGCGGAGGCCGCCGATGGTCCGA62100CGGCCTCCGCGTCTGCCGGGCGTGGTTCGCGGAGTACGGCGCGCGACCGCGTCGGCCTCG62160ACGAGCGCGTGACCGACCGGCTGCGCGMCTGCTTGGCCGGCTCTGCMGCTGTCGCCGC62220AGCGCATCGATCCCCATGTCCGACTCGAGCMTACGGTATCGATTCGGTTCGCATCGTCC62280ATTTTCAGCAGGCGCTCGGCGAGCCCTATCCGGGCATCAGCAGCGCGATGCTCTACGAGC62340ACACGACMTCGCGAGCTTGGCTGCGCATCTGGTCGCGGCGTATCCGTCCCAMGCCGCG62400CGTGGGCTGGCGAGCCCGMGACCCGGCCGCGTCGCCGGTCGAGACGGCGCTCGATGGAT62460ACGGCAGCGACGCAGCGCAGGCATTGGCGCCCCMCGTCAACGCGAGCACGATCGGCGAC62520GACGCAGCGGGCCCGGACGACGAGGATCACATCGCGATCATTGGCATCGCGGGCCGCTAT62580CCGMGGCTGCGMCCTTGCCGMTACTGGCGCMTCTCGCGGGCGGCGTCGATTGTATC62640GGCGAGATTCCGCCCGMCGCTGGTCGCTCGAAGGTTTCTACGTCGCTGACMGGACGAG62700GCGTCCCGCACCGGATGCAGCTATAGCAMTGGGGGGGATTCCTCGACGGTTTCGCGAAC62760TTCGATCCGCAGTTCTTTTCGATCGCGCCGCGCGACGCGTATCTGATGGACCCGCAGGM62820CGCCTGTTCCTGGMTGCGCCTGGGAGGCGCTCGAGGATGCCGGCTACAGTCGCGAGATG62880CTCGTCGAGCGTCATCGCGCGCGCGCGGGCGTGTTCGTTGGCGCGACCMMCAGGCTAT62940GAGCTGTACGGACCGGAGTTCTGGCGTCAGGATMGGTGTTCCTGCCGCGCACCTCGTTC63000GGCTCGATCGCGMCCGCGTCTCGTACTGCCTCGATCTGATGGGGCCGAGCCTCGCGATC63060GACACGATGTGCTCGAGTTCGCTCGTCGCAATCCACGAGGCTTGCGMCGGCTGCGTCGC63120GGCGACTGCGACATCGCGATCGCGGGCGGCGTCMCCTGTACTTGCATCCGGCCMCTAT63180GTGGCGCTTTGTTCGGGCCACATGTTGTCGGCCGMGGACGTTGCCGCAGTTTCGGCGCG63240GGCGCGGACGGCTTCGTACCCGGCGAGGGCGTGGGCGCCGTGATCCTCMGCCGCTTGGT63300CAGGCGCGGCGCGACGGCGATGCGATCCATGCAGTCATCCGGGCGAGCGGCATCMCCAT63360TCGGGCAAGACGCATGGCTACATGGTGCCGAGCCCCGCGTCGCAGCMGCACTGATTCGC63420GACACGCTGGCGCGGGCGCGCTGCGATGCCGCATCGATCGGCTATCTGGAAGCGCATGGC63480[1156]ACCGGGACCACACTGGGCGACCCGATAGMTTTCGTGGTCTCGTGCAGGCATTCGGCGGC63540[1157]GCACGCGCCGCCGGCGTGCACTGCGCGCTCGGATCCGTGAAGTCGMCATCGGGCATCTC63600GAGGCGGCGGCCGGCATGGCGGCGTTGACGMGGTCGTCCTGCAGTTGCGTCACCGTAAG63660CTCGTGCCGACGCTGCACGCAGATTCGCTCMTCCGGAMTCGCGCTCGACGGCAGCGGC63720TTTTATTTGTCGGATACATTGCGAMCTGGATGCCGCAGCCGGGCTCGGATGCACAGCTG63780CGGGCGGGTATCTCGTCGTTCGGCGCGGGCGGCGTGMCGCGCATTTGATCGTCGAGCAG63840TATCGCGATGGCGACGMGGCCCCGATCGCGGCGCTACACGACTGGATACAGCCACAGTC63900GAGGAGCCCGCCGTGTTCGTGCTGTCTGCCCGCMCACCGGGCGGCTCGATGCCTATCTG63960[1164]GATCGGGTGATCGCCTTCCTTGCGMCGGCGGCGGTCCGCTCGCGGATCTGTGCCGGATG64020ATGCAMCAGGGCGCAGCGCCATGGCGGAGCGGTGCGCCGTCGTATGCCGGTCMCGGCG64080GMCTGCTCGATATCCTGCGCATGCACCGGCGCGGCGAGCGGGTCGAGGGCTTCCATCGC64140GCCCGTATCGACGGCACAGGCGTTGCCGCCGAGGCGCCCGCATTGGACGATCTGGACGCC64200TGCGCGCGCGCCTGGGTGTCCGGCGTCGACATCGACTGGCGCTCGCGACGGGCCGCGCGC64260CGAGCGCGGCAGGCACGCGCGATTGCGTTGCCGACTTACCCATTCCAGCCACGGCGATTC64320TGGCTCGATACGCTCGCGCCTGCGCTGGCGCTTGAGGTTGATTCGGMGGCACGCCGGGC64380GGCGCGGGCGTGCCGATCATGMCGGCGGGGGGCCCACCGACGCCATCATGGGCAMGCG64440CCGGCCCACCGGGTCGAGGTGGCCAGCGAGGCAGGCGATGCCACTTCCGTGCTTCCGCGC64500GCATCGCACGCGCCCACGCCGTCAGMGGGACCAGCGCCGGCACGATCCGGCTGCCCGCG64560CCGAGCCGCTTCGTTGCGTCGAGCGAGGCGGCGTCGACCATGCCGATCACGCTCGCATCA64620CCGTCCGMGTCGTGCGAGCGCGGTCGGCGACGATGCCTGTCCCGTTCGACACGGGGGCC64680GTTGACGAGGCGCTTGTCGCGCGGTGCGATGACGTGCATCCCTCGCTGCACCACGTGGAT64740CTGCCGGTCGGGCTCGATGCGGCGGATCTCGTGGCGCGGCTGACCGCGCTGTTCGAGCGT64800CCGACGTGGGGCGAGCCGTGCGATGCGGTGTTGCTGCACAGCCCCCGGGACGGATTGCGT64860CTCTCTCTCGTCGGTGTCCCGGATGACGCGCTTGCGCGGCTGGTCGACGCGATCGGGCM64920GCGCCGTCCGTGGTGCTGGCCGCGCTCCATGGCGACGCGATCGATCTTGGTTGGGTGATC64980GGCGCCGCCTGCGATCTGATGATCGTTMCGCGGAGAGCCGCTACGGTTGCCCCTCGACA65040TCGTCCGTCGTGGCCCGTGACCTGTTGCGCGCCCGCTTCGGTGCGCGGCCTGGCTCCGCC65100GCGATGTTGTCGGGCGMGCGCGCACCGGCGCGGCGTGGCGGGCTACGMCCTCGGTCTG65160CATGCGGTGCCCGCGCTCGCCGTCGGCGAGCGGGCACGCGCGGCGATGGAGACGATCGTC65220ACCGMGGCGGCCGCGCGGCCGGTTTGCTGMGGCGCACTGGCGCGGCACGTTCTTCGCC65280GAGGCGAACACGCTCGGCGCGCCGTCGTCATGGCCGGTGCTCGCGGMCCCGMCGCGAG65340CCGGCGCGCGCGGCGGCCGCGGCGGCGGCCGGTTCCGAGCGGCCGGTGCCGCTGCCGCAC65400ACCTGTATCGAGCTGACGCTCGACGATCTCGGCGTCGCGGTCGCGCGCATGTGTGACCCG65460GACACCAAGAATGCCTTCACGCCGGCGTTCGTCGCCGCGATGGMGCCGTGATGACCTGG65520GCCGGCGAGACACCCGCATGCMGGTGCTGATTCTGACCGGCTACGGTCACTACTTTGCG65580ACGGGCGGCACCCGGCMGGCATGCAGGCGATTCAGCAGGGCMCGCGACATTCACCGAT65640GCGCGCCTGTACGAGCTGCCGCTGGCCTGCCCTGTGCCGGTGATCGCGGCGATGCAGGGA65700CATGCGATTGGCGCCGGTTGGTCGATGGGGATGGTGTGCGATGCGGTGCTGTTTGCCGAG65760GAGAGCGTCTATCACAGCCCGTACTTGTCATATGGCTTCACGCCCGGCGCGGGCTCCACG65820CTCGTGTTTCCMTGCGGCTCGGTCTCGATTTGGGCCGAGAMTCCTGTTCGGGGCACM65880[1196]CCCTACAAGGGACGGGCGTTGCGTGMCGGTTGCCCGGCCTGTCGGTCGTGCCGCGCGGC65940GAGGTGCTCGCTCMGCGCGCCGGTTGGCCGCGCAGTGGGCCATGCGACGGACACGCGAC66000GCGCTGATACGCGACAAGGGGCGTCGTCTCGCGGCACTCGCCCAGGCATTGCCCGCGATG66060ATTCGCAAGGMCTCGACATGCACGMCTGACGTTTCGTCACMTCCCGACGTAGCGCGG66120TTGATCGACCGTGCGTACGGCTTCGCTTCCACCGCGGATGCGGATACCGATGCCGGCTCG66180CGGTGTGCTCCCGGGCAGGATCTGATMCCCTGTTGAMTCGACGCTGGCCACGGAMTT66240CAGATGTCTTCCGACGAGCTCGACGATCMGCGAGTTTTGTCGATCTCGGCCTCGATTCG66300GTCACTGCCGTGACCTGGACCCGCACGCTCGGCCGCGCATTGCGGGTTGAACTGACGCCC66360GCGCACGTATATCAGTATCCGAGCATCGCGMGCTGCTCGCGTATTTGCGCGAGGCCACG66420GCCGGCTCCGCGTCGGAGGTCGCGCCGGTCACGGCGCAGGTGCAGCCCCAACCCGCGACG66480GTCGGCGCGATTGCCGMGTCGTCAGGTGCACGCTCGCGGCCGMCTTCAGCTCGACGM66540CAGGCGATCGACGCACAGGCGAGCTTCGTCGATCTCGGACTCGACTCCGTGACCGCGGTG66600ATCTGGGCGCGCMGCTGCATGAGCAGCTGTCCGTCGCACTGTCGCCGACGCAGCTATAC66660CAGTATCCCAACCTGGCCGCGCTATGCGCGTATCTCGGCGGCGAAMTACCGCAMCGCC66720GCGGAMAGACGGGCGAGGGCGTCGCGCCGACGCACACCGGCTTGAGCGTATCGGTGCGC66780GCGATGGCGGACGGTCGCGCACCGGTTCGACTGCCGCGCGTTGACGAGGAACGGCCGACG66840GCACCGGTCGATCGGGACAGCGTCGCGCATAGCCTCGCACACGMCTTCAGCTCGACGTG66900ACGGAGATCGATCCTCGGACGAGCTTCGTCGACATGGGGCTCGATTCGGTGACGGCMTC66960ACCTGGTCGAMGCACTGACGGTGGCGCATGGCGTCGAGGTCCGCCCGACTGACCTGTAC67020CGGTATCCCACCCTCGACGCGCTCGTGGTTCATCTGTCGCGCGCGCCCCGMTCGCGCCC67080GTGCGGGCGGCMCGTCCGCCTCGATGACCGAGGGTCCGGCCGGGTCGCCTGTCACGCGC67140GGCCAGCGCGGTCCTGCGGCGGCGCCGCCCCCCATGCCGGCCGCGCCGCTTCGCGACGCA67200TCGCGCGMCGTGTCGACATCGCGATCATCGGGATGGCCGGCCGCTTCCCGGGCGCGGCT67260GATCTCGACGCATTCTGGCGCMCCTCGTAGAGGGACGCGACAGCATCGTCGAGGTGCCG67320CCGCACCGCTGGTCGCTCGATCGCTATTACGATCCCMTCCACAGTCAMGGGCMGACC67380TATAGCCGCTGGATCGGCCTGCTCGACGATGCCGACGCGTTCGATGCCGAGTTCTTCGGT67440ATTTCGCCGCGCGAGGCGGAGCATATGGATCCGCAGCAGCGCGTGTTCATGGAGMCTGC67500TGGCACGCGATTGMCACGCGGGCTACMCCCGTTCMTCTCGCGACACGGCGCACGGGT67560CTGTTCGTCGGTTGCGCGACCGGCGACTACGCCGATCTCGGCCMCGCGATGAGGCCAAC67620GCACMTCGTTCATGGGAGGGGCGAGTTCGATCCTGTCGGCGCGCATGCCGTATTTCCTC67680MTCTCGACGGTCCATGCCTCGCCATCGACACCGCGTGTTCGTCGGCGCTCGTCGCGGTC67740MCGAGGCCTGCMCAGCCTGTTGCTCGGCMCTGCGACTTCGCGATCGCCGGCGGCGTG67800TGCGTGATGGCCGGTCCGACCCTGCACATCCTGTCGAGCAAGGCCGGCATGCTATCGCCG67860ACCGGCCGCTGCCGCACCTTCGACGAGGCAGCTGACGGCTTCGTCCCGGGCGAGGGCGTC67920GGGACCGTACTGTTGCGCCGGCTCGACGACGCGCTCGCCGCCGGCGATACGATCCACGGC67980GTGATTCGAGGCTGGGGCATCMCCAGGACGGCCGCACGAACGGCATCACCGCGCCGAAT68040CCGGATGCGCMCGGCGGCTCGCGACCGGGATCTACCGACGCTTCGGCATCGATCCGAAG68100CGGATCACGTTGGCCGAGGCGCACGGCACGGGCACTCGGCTCGGCGACCCGATCGAGGTG68160CGCGCGTTGACCGACGCGTTTCACGAGTTCGACGTCGGCAACGCCTACTGCGCGATCGGT68220[1235]TCGGTCAAGAGCMCGTCGGACACCTGGCGCTCGCGGCGGGMTCGCGGGACTCCTCAAG68280GTGGTTCTGAGTCTGCGGCACAGAACGATCCCGCCGACGCTCCATTGCGACACGGTCAAC68340GAGCATATCGACTTCGCGCGTTCGCCGTTCCATGTGCCGCGCGTCGCGCAGGCCTGGTCC68400GCAGCCGACGGGGMCCCCGGATGGCTTGCGTCAGCTCGTTCGGCTTCAGCGGGACCAAC68460GCCCACCTGGTCGTCGMGAAGCGCATGCGGTTGCGCCGCCTGCGATCGGCGACCMTCC68520GCACGCGAGCATCTCGTCGTCTTGTCGGCCCGGCGGCCCGAGTCGCTACGGGCCGCCGCG68580MGCGACTGCGCGACGCGCTTGCGGCGCMGACGCCGCCGGTTCGGCTCCCCGCCTGATC68640GACGTTGCCTACACGCTTCATACCGGGCGCGCCACATTCCGGCATCGTCTCGCGATTGTC68700GCGTCGTCGCTTGACGGCCTGATCGAGCGGCTGGATGGCTGGCTCGCGAGCGGCCCCGAT68760GATCAGGCAGGCGTCCACGACGGCACGGCGCCGACATCTCACTCGGCCGAACACTCGGCG68820CGCGTCGCTCAGGCGCTGGCCGACGGCGATCCGCGCGCGGCGGCGGCGCTCTGGGTCGTG68880GGTGGCGAGGCGGGTGGCGAGACGCGCGCATTCGATGGACTGTCGCCACGCCGGATCGCG68940TTGCCGGGCTACGTGTTCGTGACGTCGCGTTACTGGGTCGGCGGCGCACCGCTTGCGGCC69000GGCGCGCCGCGCCCGGCCGCATCAGMTCGCAGGCGACATCGATCGCACGCGGCTCGGGC69060MGCGMTCGCCGTGGTCGGCGCTGGTCCCGCGGGGCTGGTGATGGCGMATCGCTGCTC69120GAGGAGGGACATCGGCCGGACGTATTCGMAGGCAGGCCGATCTCGGTGGCGTCTGGCTG69180TTGCGTGCCGAGMCAAGCGCGCGGGAGCCTACCGCMGACGCGCTTTCAGACGTCGAAG69240TACACGAGCGCGTTCTCCGATTTCGATGGAGCGCCGATCGACGCGCACTTCCACGGCGTC69300GCCGACATTCATCGCTATCTACGCGATTACGCCGAGCGCTTCGGTGTMCGGCGCGTATC69360CGTTATCGCACCGAGGTCAGCCGGATCGMCCGCACGGAGATCMTGGCACGTCACCACG69420GTGTGCGACGGCGCATCGCGGACCGATGTCTACGACGGCGTTGCGCTGTGCCAGGGGCTC69480TACTGGAAGCCGTGGCGCCCTGAGTTCGCCGGCCTCGATACATTCCGCGGCACGATCCTG69540CATTCGGCCGMTATGTCGACGAGGCTTGCCTTMGGGTAAGCGCGTGCTCGTCGTCGGC69600MCGGGATCAGCGGCATGGACATCGCCGAGGAGGCCACGCAGAGCGCGAGCCAGGTGTTC69660TGGGTCATGCGCCGGCCGMATTCATCATGCCGCGCATGACGGGTTTCGTGCCGMCGAT69720TTCCAGTCGCCTGCGAACCTGCTCGCCMCGTCGATCCGGCTCAGATCATGGAGCGCGTG69780CGCCATTCGATGCCCGAGTATTTCCGACGGTACCAGGAMGCGGCCTGCTGCCCGACGCC69840GACGACTTCCGTCGGCAGCCGGTCGTGCAGATCMCGACGGCATCGTTGCGCTCGTCGCG69900AGCGGTCGTGTCGAGGCGGTGATCGACGAGATCGAGGCGTTCGACACGCACGGCTGCCGG69960TTCCGGAGCGGGCGCCGCATCGAGGTCGACGCCGTCGTGTTCTGCACCGGCTACCAGATC70020GACGMCGGTTCGATTACCTGCCGGGTGTATCMTGCGCGCCGACTTCGCGATGGGGATC70080TTCCACCGCGMCGACCGAGTCTCGTCTCGACATCGTGCCCGCTGCCGGTCGCGTATTCC70140GGCACGTTCTTCTTTCCAGAMTGGTGGCGCGTTGGTATGCGCGCGTGATGTCGGGCCGT70200TCGGMCTGGCCGCCAGCGAACTCGACTACCGCCTCGACCCGCGCCACGCCGCGATCAAC70260GGACCGATCGCCMCGTGGTGTTTGGGCTGCGGCTCGGCTTGCTGCCCGATCCGGCGCGC70320GMTTCCGGGCGTTCTGGCAGCTTTTGMTCTGCCTCCGTTCCCGCCGATCTACCGGCTG70380CGCGGCGAGCATTCGGATCCCGACGCGCCCGCGCGCCTGCAGGCGCTGMCCGCCGCAAC70440CTGACGAGTGGCAMGCAGACGCGGCGCTCGACGTGGTCCGCTACCGTCTGCTCGCCGGG70500[1273]CTCGGCGATGCGACGTTGCGCGACTTGCTCGCGCGCGGCGAGATCGATGACMCGATTAC70560[1274]CGCMCGCCGGCCGGCACGCGGCCGATGCGATCACGCTCGACTGGAGGCGGCMTACATC70620CTGCGCGATGACGATGTGGCGCAGGCCCGGAGAGMCCTGCGGCGCGATCGGAGCGCGCG70680CCGCACCCGGGCAGCCCGGCGTGGMCGACTACCAGACGCTGCTGGCTCGACTGAGGGCC70740GGGGMCTGGACATGGMGGCGTGTTGCACGCCTTGACGAAACAGGACGACTGATATGAC70800CGAGMCTTCGAGMCTGGTTCGATCGACTGCGCAGCGACACGCTGGACGAGCGGGATGG70860GATCGCGTTGCTCGCGAGTCTGCGCGACGCGTTGCGCGGAAGTGGCGCCCGTGCCGCGGC70920CGTGCCAGGGGACCGTCTCGACGGGGCGCGTCTGCCGCGCTTCGCGACGCCGGATTGGCA70980GGCGCGATCGTATCGCGAGGAGCGGCAGGTGGCGCCGACGTTTGACGCGGTACTTGTGGT71040CGGCGAGGCCAGTCTCGCGGCCGACTGGCGAGAGGGACTGACCGCGGCCTTTTCGCCGGC71100GCGGATCTTATGGATCGACACCGGCGCGCAGCCGCCGGAGGCCGCGCACGAGGATCCCGT71160GCCCGATGCTGTGGCCGAGAACGCCCGCGTGGCAGTGCTGCTGATCGACGATCTCGCGCA71220GGATGGCACCGTGGGGGAGGCTGACGAGCCGCATCGTTATCTGACGCGCTTGCTCTGGGC71280ATTCGCGTTGCTGAGGMCCTGCTCGCCCGGCGTATCGAGCGCTGCCATCTGCTGCATCT71340GACGCCACTGCGTCATGCGCTGCCGATCGCCGCCGCATGGCATGGGCTTGCCMGAGTGT71400CGTGCTCGMAGTCCCCGCTTTGCGAGCGGCGGTGTCTGGCTGTCCGACTCAGACGCGGC71460CCGCGTGACACCCGAGTTGGTCGCACGCGAGCTGTCCGMCTGATGCCCGCGCGCCATCG71520AGACATTGCATGGATCGACGGCCGGCGCTGTGAGCGCGTGCTGGTCGCCCACGCGCATGG71580CGCCGTCGCCCCGACAGTCGGACCGTTGGCGTGCGGCGAGACGGTTTTGATCACGGGAGG71640GGCGGGCGGACTCGGTCTCGCGATCGCGGAGCGGCTGACGACCGATTTCGCGCTGCGCGT71700CGTGCTGTGCGGCCGCCGCGCGGCTCACGCGCTGGATGACCGACAGCGCGACTGGCTCGC71760GCGGCATCCGACCGTGCGTTATGTGAGCGCCGACGTGAGCGACCCGGATCAGGTGCGCAC71820GCTGATGGCGGAGCTCGGAGCGGCCACGCCGCTCGCCGCGGTCTTCCACGCGGCGGGCGG71880TAGTGACGACGCGCCGCTGCGTGCGMGTCGTTGCGCGCGATCCGCGCGGTGCTCGCGCC71940GMGGTGCTCGGCACGCAGTGGCTCGATCGCCACGCGATCGGCCTCGGTGTGCGCTACTT72000CGTTTGCTTTTCCTCGGTCGCGTCGCTGGCCGGCAGCGTGGGCCAGGCCGACTACGCCAG72060CGGCMCGGTTTCATGGACGMTACTGCCGGCTGCGGCAGGCGGCCAGCGATGCATCGGG72120CGGCTGTTGCTACCTCAGCATCMCTGGCCCTACTGGCGCGAGGGCGGCATGCGCGCCGA72180TCCGGGCCGCCTCGMCGGATCGCGGCCGACAGCGGCCTCACGTCCCTCGGCACGGTCGA72240GGGTGTCGACAGCCTGCTGGCCCTCCTCGCGCGTCCGGCACCGCAGGTCGCCGTGCTGCC72300GGGCGAGCGTGCCAGGATCGACCGCGCGTTCCTGGCGACGCAGCCGCCGGAMGGCCCGT72360CGCTCGCGCCGAGGCGGACGATCCGGCCTCGGGATTGCGCGMCTGGTTGCGCGGACGCT72420GMGATGGCGCCGGAGGCGATCGATGCCGACACGCCGTTCAGCGAGTATGGCTTCGACTC72480GATCCTGTTGACGGAGCTGGCMGCACCATCGGCCGTCGCTATCCGGGGCTGGMCTCGA72540AGTCGGCGTGTTCCTCGATCATCCCACGCTGCGCGMTTGAACGACTGGCTACGCGTTCG72600GCTGGCGGACACATCCTCGGATCCCGACATCGCGCGAGCCGCGCGACCGGACGMGTGGC72660GCCGTCCCAGGGCGACCCATTGCTCGACAGGTTACGCGCGCTGGCCTCGACGATCCTGM72720ATTGCCGGCGACTGACATCGACGCCGAGACGTCATGGTCGACATTCGGCTTCGATTCGAT72780CAGCCTCMCGAGTTCGCGGTATTGATTGCGCGTGATTTCCGGACCGGACCGCTCGCGGC72840GGATCTATTTCTGTCCTGCGCGACGCTCGCGGAGTTGGCTGCGCATCTCGCCGCGCGCGT72900[1313]ACGCGCCACCGAGCCGGCGCACGAGCGGGCGCCGGCGCGCCGCGTGGCGGTGTCGGCGCT72960GACGMTCCCGGCCCCGTACCGGACGCGACCGAGGAMTCGCGATCATCGGCATGGCGGG73020GCGCTTTCCGGGCGCGAGCGATCTGCCCGATTTCTGGCGCAACCTCGTCGAGGATCGCCA73080TGCTGTGACGGAGATTCCGGCGGACCGCTGGCGGTGGCAGGACTATGCGGCCGGCGCGCC73140GGGCMGACCGATTGTCATTACGCGGCGTTCCTGACCGCGATCGACCGTTTCGACGCCGC73200GCACTTCAGCATTTCACCGCGCGAGGCGGAGTTGATGGACCCGCAGCMCGACTGTTGCT73260CGMTGCGCATGGGAGGCGTTCGAAMTGCGGCMTCGCGCCCGACTCGGTCCGCGGTCG73320MGGATCGGACTATTCTTCGGCGCGGAGMGCACGACTACCTGTCGTTGATCGCCGACGC73380GGACGTCGACATCGATCCCTACATCMCACCGGCMCGCCCACGCGATGCTGACCMCCG73440CGTCTCATACTTCTTCGGCCTCACCGGCCCCAGTATGACCTTGMTACCGCGTGCTCGAG73500TTCGATGACGGCGATCCACGCGGCGGTCGCGAGCCTGCGCAGCGGAGCATCCGAGATGGC73560GCTCGCGGGCGGCGTGMCATCTTGTTGAGCCCGGGGCTGTTCGTGCTGAACCGCAMAT73620GGGMTGTTGACCGCCTGCGACCGCATCMACCGTTCGACCGCGATGCATCCGGTCATCT73680GTTCGGCGAGGGGCTCGGGCTGGTGCTGCTGAAGCCGCTGCGTCTTGCGTTGCGCGATGG73740GGATCCCGTGCATGGGGTGATTCGCGCGGTCGACGTGACGCACGGCGGCCATGGCCGATT73800TCTGACCGCGCCCCACGCGCCGTCGCACGAAGCGTTGATCCGGGGCGTCCTTCACCAGGC73860CGGCGCGTCGCCCGCCGACGTTGACTATCTGGAGGCGCAGGGCAGCGGCGACGCGTCCAC73920CGACCGCATGGAGCTGGAGACCTACCACGCGCTGTACGGCGACCGGGCGGGCGGCGAGCC73980GCTACCCATCGGCAGCGTGAAGGGGCATATCGCTCACCTCGGCGCGGCGTCGGGCGTGAC74040CGCACTGATCMGGTGCTGCTCTGCATGAGCCACGACCGGCTGCTGCCGATTCTCCATCA74100TCGCCAGATCMCTGGCCGCACGACGAGCCGTTCGCGGGCCGGCTGCTGACCGCGCATGC74160CGMTGGCGTCCGMGTGGGTCGATGGATGCCGAGTGCCGCGCGTTGCCGCGGTACACM74220TTTCGGCTACGGCGGCGTGAACGGGCATCTGCTGGTACGTGMTATCTGCGAGCGCCGGC74280TGTCGAGGCGAGGTGGCCGGCCGTCGCGCGGCTTTGGGTGGTGTCAGCGCGCACGGCCGC74340GCMTTGCGGCAGAGCGTCGCGCGCCTCGTCGCCTACCTGCGCGACGGCGGATACCGGCT74400GCATGGTCAGCGTGAGCCCGGAGTCGAGTCGATCGCATTCACGCTGCAGACCGGCCGGCA74460GCCGCTCMTCATCGCCTCGCCTTGTGGATCGGCGATACTTTCMGGTCGACGGGCTGCG74520CACGGCGCTCGCACGGCTCGACGCGTGGCTCGCGGACGGACACGGCGACGCGCATCTGCT74580GACCGGTGTCGTCACGCCCGCGAGCCGCGTCGAGCCCAGCCCGGCCAGCGACGAGGCAGC74640AGACGATCCCTTGCGCATCGCCGCGCGCTGGATCAGCGGCGCGCCGATCAATTGGACCGC74700CTGCTATGGCGACACGCGGCCCGCCCGGATCAGTCTGCCTGTCTATCCATTCGCCGCGAC74760CCGGTACTGGGTGGCGTCCCGCMAGACGGCGACGGAGCGGCTCCGCAGGGCGGCTCGAC74820GGCGCCAGGCGCAGCCCCGGCTCTCGCCGGCGTGCCATCCGCTGCGCGTGCCCGGCCTGC74880AGCCGTCCTGCATCCGTTGTTGCATCGCCTGATCCCGGCCGATCGCGCGCTGCGTTTTCT74940CAGCACCTTTTCCGGCGACGAGCCGTTCTTTCGCGACCATCGTGTGCGGAGCGCCGCGAT75000GCTCCCGGGTGCGGCGTATTGCGAMTGTTCATCGCGGCGGCGACACTCGCGATGCCGGA75060ATCCGMCGGTGTTCGGCCGGATTCCGGCTCGMCACCTCGTTTGGACCCGTCCGCTTGT75120CCCGTCCGACGGTACGGCCGACATTGCGGTGACGATGGAGCCGGCTATGACGCCGGCTCG75180TCGTGCCGGCATAGACGGCCGCGATGTGTTTCGCTTGACCGTCGGCGATGTCCGCAGCM75240[1352]CGACGCGCMCCCTATGCCCMGCCATCGTCGTGCCGGAGCAGCGCGACACGATGCCGGT75300TCCACCCGTCGTCGCAGTTGAGCAACTGGCCGTGTACTCGCGCGGTACACGGATCGACGG75360GGCACGGCTGTATCGGCGCTTTGCCGMTTGGGCTTCGMTATGGTCCGAGCCACCGGTG75420CATCGACGCGCTGTTCTACGACGGCGACGAAGTGCTGGCGCGCATGCGTCCGTACGMCC75480GGACACGCACGGCACGGGCTACCTGCTCGCTCCCGGCCTGCTCGACAGCGCTTTGCAGGC75540CTCGATCGGCTTTGATCCTGCAGCGCTCGACGCGMCCATGATGGTTACCTCGACGGCCG75600GCTGTCGCCGTTGCTGCCGTTTGCGCTGGAGCGACTGCGTTGCCATCGCACGTCCGGTCT75660GTCGGMCACGTGCGCTGGGTCAGGCTGCGACGGCGCGCGGGCATGGCCGGCMCCCGGT75720CGTGMGCTCGATGTCGATCTGTTGGACGCCGACGGTCATGTCTGCGCGCGCCTGGAGGG75780GTACAGCGCGCGACGCGTGAAGTCGGMGTCGCGGCTTGCGCCGTCCCCGCGACGCGTCG75840TGATGCGGCCGGCACGATACCGGTGCCGTTGCCCGAGCCTGCCGTTTGGCGCGACGAGGA75900CGGTGTGCGCGTCTACTCCTGTCGTTTCGAACCGMTGACCTTTATCTGCGCCATCATCG75960GATCGATGGGCMGCCCTGTTACCGGCGGTCGCGCTGCTCGATCTGGCGCGGGTCATCGC76020CGACACGCTGCCTCGCGACGAGGCTGCCGCGGCACTGCGCATCAGCCGGGCCACCTGGCA76080GMGCCGGTATTCGTCGGTACGCCGGGGCGCGATGCAGCGCTACGCGTCGCCCACCGCGA76140TGGCTGTTGGACGGCTACGCTGGAMGCGGCAACGGCATGCMGGAGGCGTGGCCCCCTA76200TGCGCMTGCGATCTCACCTGGACCGCCGACGTTCCCGACGCCGACCCGTTGCCCGATCG76260CGCACGCCGTGCCGGCGCGCCGCTCAGCMGGCGGACGTCGCCCAGCGCTTCGCTGGCTC76320GCTCGTCCGCGMTCGGGCAACGTCTTGGGCCTGCCGGGTGGMTCCGGATCATCGGTTG76380CGAGAGCAGCGCCGACACGACTTGGCTGACGCTAGCCTGTGMGATGGMACGACGACTC76440GGCCGGCGGCGCGCGCTCGACCGCGTTCGGCCTACTGTTCGCGATAGTCGACTTGTGCGG76500ACGCGACCGTGAGGCGGCAGGACTCGCCATCCCCTACGGGATCGACGMTGCTTGCTGTA76560CCGGGCCTTTCCCCGCCAGGTGCGGGTCGAGATCCGTGCAGCCTCACCGGGCACCAGTGC76620GCGCACGCGTGTCTACGACATCCGCATCGCCGATCTCGAGCATCGACCCGTGATGCMAT76680CACCCGGCTCACGATGGTCGCGCCGCGCGAAGCAGCGGCGGGTCCGGCCGCGAGCCCGAC76740CGCTTTGACCGATGACGCGACGCTGGCACTGCMCMTGTCTGCGCGMTGGGTCGGCGA76800ATTGCAGAMATTGATCCGGACGAGATCGGACTCGATGACGATCTTGCGACGTTCGGTCT76860CGACTCGTTCAGCTTCACGGCGCTTTCMGCCGTTTGMCGATCGTTTCGCGATCGACAC76920GATGCCTACCGTATTTTTCGAGCATGGCACAGTCCGCGCACTGGCGMTGGCTTGCTGCG76980CACCTATCCGMCCTCGCGGCCGGTCTGCGAGACGACGGTCGCGTGACACGGCGGGACGA77040GCCGGGGCCAGGCGCGATCTCGCCGGTMGCGCTTCATTGCCGCGAGGGCCGGCCGGAAC77100CGACGCGATCGCGATCGTCGGCATGGCGATTCGTCTTCCACGGGCGAAMCGCTCGATGC77160ATTCAGGCGGCGCCTGATCACGCCGCACGAGGAGGCGAGTGGCCTCGAGGCGGCTGCTTC77220GMTCGGGCCGATACGGATAAGMCGGCGCGGATGCGGCGACACAGGCCGATCGGGTCCC77280GCCAGCATGGTTCGGCGCCGACGTCGAGGAGGGGGCGTCCGGCCGGGGGCTCGTGTCGGT77340GCTCGMACCGTCTGGCACCTGTTCGAGGACGCGGGMTGGCGCCGGCTACGCTGCGCGG77400TAGCGATACCGAGGTCTACCTGGGCGTCTCGTCGCGCGCGACGGCGGAGAGCGATCTGCC77460CGTTGCGTTGCCCGCATCGCATGCATTCTCGAACCGMTCTCGTTCGMTTTGATTTTCA77520TGGCCCGAGTCMGCTATCGATACGGGCTGCTCGAGCTCGTTGGCCGCGCTTGCGCGCGG77580[1391]TGCCGMCGCCTGAGGCAGCGGCGCTGCGGGCTCGTGGTCGTCGGCGGCGTGMTGTGAT77640CGCGCCAACGTGCGGCGGGGAGGCTCGCGCATCGGACMCATGGACGGTGGGATCGGCAT77700GGTCCTGCTGMGCGTCTCGACGACGCGCTGCGCGATGGCGACCGGATTCATGCCGTGGT77760CGCCGCGGTCGCCGAGMCCATGGCGGACGTACGCTGTCCTTGGCCGCGCCGAGCGTCGA77820GGCACAGCGCGMTTGCTGATCGCGGCGTATCGTCAGGCGGGCGTCGGCGCGGCTGGGAT77880AGGCCATATCGMTTGCATGGCGCCGGCACGGCGGTGGCCGACACGATCGAGTGCGMGC77940GCTGCGCGACGCGTTCACCGMCTGCGGGGACGGCMGCCGTGCCACGGGAGGCGCCGCG78000[1398]TTGCATGCTCGGCGCGATCGGCGGACGGCTTGGCGCACTCGGCGCGGCGGGTGGCATGGC78060CGGCGTGATCMGACAGCGCTGATGCTGCGGCATCAMCAATCGCTGGCCATCCGCCATG78120GCTGGACACGCGCCTCGTCGATCTCGMGGCAGCGGCTTGGMGCGGTCGGCGAGGCGGC78180GCGGCCATGGCCCGCGACACACMTGCCCAGCCTCGCTGCGCGGGCGTCAGCAGTTTCGG78240CGCGACTGGCGCGMCGCGCATGCCGTGTTGTTGGMCATGTGGCGGTCCCGGCCCATCG78300CCCCGATGGGATGTCGCCGCCGGATTCGGCCCGCCTGTTCCTGCTGTCCGCGCACAGCGA78360MCCGCGCTGCGTGCGCAGGCTCGCGCGCTCGCGTCCCATCTCGCGCGGTGCGCGTCGGC78420GGACGACATCATGGCCGATGCGCGAGGGCAGGTGCTGCACGACATGGCGTACACCTTGCA78480GGCGGGACGCGACGCGCTACCGTGGCGCTTTGGCGTAGTGGCGACGACATCCGACGCGTT78540GCMCGACAGTTGCGGCTGTTCAGCGATGATCCMCGGAGGGGTGTGCGGCGCTCGCCGA78600ATTCCGCGTGGCGCACGTCGATCCGCTCCGCCCTGACTTCTCCGACGACGAGGATATGCA78660GGCGCTGGTACGGCMTGGCTTGAGAMGGGAMCGGATGCACGTGCTGCGTCTCTGGCT78720CGATGGCGTCTCMTCGATTGGCGAGCAGCCTACCGTACGCGCCCGGGTGCACTGACCGA78780CCTGCCGCCGTATCCGTTCCCGAGCGCGGTGGCGCTACACGMGCCGGCACGGACTGCGC78840CGTTGCCGCGGCTCAGACGCCGGCTCGTGCGGCGGGCGATCTCGCGGTCGACGTACGCAT78900ACGTCGCCTGCTCATCGGCGGACCGGMCGCCACGCGTCCATTGACGGGAATTCGCCCGC78960CGATGCAGAGGCGCTCATGCMCGCATTCGCCACGTGGGCGAGTTGCTCGGCGCCACGTT79020GAGTGTCGGCGATGCTTACCGGTACCCCMTGTCGACGCGCTGCGCACCGCCTTGCGCGA79080TCGTGMCGGCGCGCGGAGGCTGCGGATGGAGTCGCGGCCGCGGCTTCCGGCGATCCGTT79140CGCGCGCTACTACCGGATGGCGTTTGGTGAMTCGATGGCTTCGAGCGTGCGCTACTCGA79200ACTGGACGACGATGTCATCCTCGACGTTCTGCACMTGTCTCGCCGGGCAAGCCGCCGCT79260CCTGCTGCTGGCGCCCATGGGCTGCCTGGGTACCGCCTGGCTGCATCAGGTGCGCGMTT79320CGCAGCCGACTACTCGATCATCGTGTGCCATTACCCAGGCCACGCGGCGAGCAGCGGCAC79380CGCCGCGCTGTTGTCCGCGGCGGGGACACTGGCAGACGTCGCCCGGTTGATCTGGACGGC79440GCTTGATCGACTCGGCATCGGCTTGCCCGTTCATCTCGTCGGCTGGTCGATGGGGGGGCT79500CGTTGCGGMTGCATGACAGCCGTCGATCCGGAGCGGGTAGCGTCGATGACGCTGGTGM79560TGGGCTCGACGCGCCCGGCAACGGCGGCCTCGACCGCGCGATGCGCGAGTTGGTGCATGA79620GGTCTCGACGCAGGTCGCGCCCGAGGTATATCGCCATTTTGAGGGCGACCACTTCGCGAT79680CMGGCTTGTTATGACGCGAGCATCCTCGATGCCTACCTGCGTTGCGCCAGCGCTACCAC79740GGTGMGMGGATCTCGCCGCGCCGAGGTTTCCGGTGCTGGTACTCGCGGGGGGGCGCGA79800TCGCGTGATTCCGCCGGAGGCGTCGAGGCGCCTGTGGGAGCGCTTCGACGGCGCCGCGTT79860CGAGTTGCTGGACGMGCCGGACACTACTTGCCGATGACGCATGCGGACTGGTTCMCGA79920[1430]GCGGTTGAGGCAGCATCTCGMGTGACGGTCTGACCGTCCGAGCGCGTCGGCGCGCTCTT79980TCCACMGACGCATGCATCGAGTGAGTCATATCATGAGMAAGTCTTTCTGTTCCCAGGG80040CMGGTTCTCAGCGCCGCGGGATGGGGCGCGAATTATTCGGTGCATATCCGGCGCTCACG80100GCATGCGCMGTGCCCTGCTCGGCTATGACATCGCCGAGTTGTGCCTCGACGATCCACGC80160CGCGMCTTGGTCAMCACGTTTCACGCAGCCGGCGCTGTACGTCGTCAGTTGCCTCTCG80220TACCTGAACCGMTCGACAGCGGCGAGCGCTCGCCCGACGTCGCCGTTGGGCACAGTCTC80280GGCGMTTCGCCGCGCTGTTCGCGGCCGGCGCATTCGACTTCGAGACCGGCCTCGCGATC80340GTCGAGAAGCGGGCGGCCCTGATGAGCGACGCGCCGGACGGCGCGATGGTCGCGGTGCTC80400GGCATCGATGAGCMCGTGTCCGGGAMTACTGGACCATCACCTCGCCGAMTTCMGAC80460MTGCATCATCGCCGGMGTGGAGATCGCCMTCTCMTCATCACCTTCAGTGTGTGATA80520TCGGGTACGCGCGAGCGACTCACTGMCCAACGCTCGCGCGGTGGTTTGAAGACGCGGGT80580GCGAGTTTCATTCCGCTCGATGTCGGCGGCGCGTTTCATTCCGTACGTATGMGGGGCCG80640GCCGCACGTTTCGCCGACTTCCTGTTGAGCGTCGAGTTGCGGCCGCTGAGMTGCMGTC80700GTCTCGAACTGGACGGCACATGCTTATCCGGCCGGCGATTACCGGCCMTGATGGTGAAT80760CAGATGTTCAATCCTGTGCGATGGCGCGATTCGATTGCATTCCTGCTCGCGCGCGGTTAT80820CGCGACTTCCATGAGGTCGAGCCGGGCGATGTCCTCAGTCGGCTCCMCGCCATATCGM80880AGCGAGCAGCCGCACGGCATGTGACGCGCTCCGTATGMTTTCCAGAGMGGCGAGAGM80940GMCGTGMCACGGTCGGMTCGAAGCMTGAATCTGTATGCCGGTGTAGCCAGCCTGGA81000CGTGACGGCGCTGGCCCGTCACCGTGGGCTCGACATGCGGCGCTTCMCAATCTGATGAT81060GMGGAGMGACGGTCGCGCTGCCGTACGAGGACCCGGTGACGTTCGCGGTCMTGCCGC81120GATGCCGATTGTCGACGCATTGACCCAGTCGGAGCGCGACCAGATCCGGATGGTGATCAC81180CTGTACGGMTCGGGCTTCGATTTCAGCMGTCGATGAGCACGTATTGCCACGATCTACT81240CGGGCTGAGCCGTCACTGCCGCATGTTCGAMTCMGGGCGCCTGCTATTCCGGTATGGC81300CGGTCTGCAGATGGCGGTCAATTTTGTTCTGTCGCGGACGTACCCCGGCGGAMGGTATT81360GGTACTGGCAAGCGATGTGTCCCGCTTCATGGTGGAGMGGGGGGCGATGCGTTATCGGC81420GGACTGGTCGTTTTCCGAGCCGAGCGGCGGCTCAGGCGCGGTCGCGATGCTGGTCAGCGA81480CCAGCCGCATGTGTTCCAGATCGATGCCGGCGCGMCGGCTGCTACGGTTACGAGGTGAT81540GGACACCTGCCGTCCGGCGGCGGATGCCGATGCCGGAMTGCGGACCTGTCGTTACTGGC81600ATACCTCGACTGCTGCGAGAATGCGTTCCTCGACTATCGCAAGCGAGTCGCCGACGCGGA81660TTTCGCGACGACGTTCTCGTATCTCGCATTTCATACGCCATTCGGCGGCATGATCMGGG81720GGCGCATCGGACGATGATGCGCMGCTCGTGATGGCGMGCCGCCCGAGATCGAGGCGGA81780CTTCGATCAGCGCGTGGCGCCGGGCCTCGCCGACTGCCAGTCCGTCGGCAACATCATGGG81840TGCGACCGCCGCGCTGTCGCTCGCGAGCACCATCCGCCACGGCGACTTTTCCGAGCCGCG81900GCGTGTCGGCGTGTTTTCCTACGGCTCCGGCTGCTGCTCCGAGTTCTTCAGCGGCGTCGC81960GACACACGMGGCACGCACAGGCTGGCCGAGCTGGAGATCGGCGAGCAGCTCGATCGACG82020CCGAGATTTGACGATGGCTGMTACGATGACGTGCTCGAGCGMGTCGCGAGATCCGCTT82080CGGCACGCGAMCCTGGACGTCGATACTGCGCTACTGGCAAGCGCGCGCGGCGTGCATCG82140CCGGCCAACGCTGTTCCTGAAGTCGATCCGGGATTTCCATCGGGMTACGMTGGGTGM82200CTGMCGGAGCGTGGCGGMCTTGACCGTCCAGTTCGACGATGGCATCTGCCGGATACGG82260[1469]ATCGATCGCCCCGATACGGGCMCGCGATCGATGCGCGTCTGGTCGACGAMTGGACAGC82320GTGCTCGACGAGTACGMGCATCCACACGCATCGTCGTGATCGMGGTTTGCCCGACAM82380TTTTGCGTCGGAGCGGATTTCATGCMCTCGACGCMGGTTGACGACGGGCGATTCGCTG82440MGGCGCCGGATCCCGCACCGCTTTACGATTTGTGGACGCGGCTCGCGACAGGCCCCTAT82500GTCAGCATCGCACATGTACAGGGACGAGCCMCGCGGGCGGCGTCGGATTCGCGGCCGCG82560TGCGATGTCGTGGTCGCCGCACAGACTTCGAGCTTCAGCTTGTCCGAGCTGTTGTTCGGG82620CTGATGCCAGCGTGCGTGCTGCCGTTCCTGATACGCCGCGTCGGCTTCGCGAGGGCGCAC82680TACATGACGCTGACGACGCAGCCCATCGGCGCAGCACAGGCGCGCGMTGGGGGCTAGTC82740GACGTTTGCGGCGCTGACAGCCGTGATCTGCTGCGTCGGGATCTGTTGCGGCTTCGACAT82800CTGAAMAGGATGCGATCACACGCTATMGCGCTATATGGCCTCGCTCGACGACAGCCTG82860GCCATGTCGCGCGCGCCGGCGGTCGCAGCCMCCTCGMGTGTTCGGCGACGCGGACAAC82920CTGCMGGCATCTCGCGTTACGTCMCGCGGGAMGCTGCCGTGGGMCCCGACTGACCC82980GGTCTGCCGCCGTGGTCCGCGCCGACATGACGTGGCGCACAATCCGGTACGAGCCAMAG83040GAGAGAGGMTCATGAGTCAGCMCAGATTCTGGMTTGATCGCGAMCACACCCGGGM83100ATTGTCCCTGGGCTCGMGATCATGTGTTCGCACCGGGCGATTCACTGTCCMCCTTGGC83160GCGMCTCGGTCGATCGCGCCGAGATCATCGTGATGACGCTCGAGGTACTGGCGCGGGAG83220ATCCCCATTGCCMGATGGCGMCGCGCAGMCATCGGCGAACTCGCGAGCATCATCGAT83280GCGMTGCGTGACGATGCGGCMTCGTCGTCAGCGGCGTCGGCGTCGTATCGGCGATCGG83340GCAGGGCCGCGCGGCATTCATCGATGCGCTGCTCGMGGGCGGCACCGGTTCGACACAAT83400GCGTCGTCCGGGMGGCAGTGGCGGCCGMTGGCGACGTCCCGGCMCGGCGTTTCTCGG83460CGCGGAGATCGATGCCCTGACCATCCCGGCGACGGTGTCGCGCGGCTTGTCGCGCACTGC83520GTCTTTGTCCGCGCMGCGGCGCTCGCCGTACTCGATGMGCATGGCGTGACGCGTGTCT83580CGGCGACGTCGATCCGGAGCGCATCGGTCTCGTGATCGGCGGCAGCMCATGCAGCMCG83640CGMCAGGTTCTTCTGCAGGATGCCTATCGCGAGCGTGCCGGGTTCATGCGCCCGACCTA83700CGCGATGTCGTTCATGGACACAGACATGTGCGGCATCTGCTCCGMGCATTCGGCATCCG83760CGGCTTCGCGCAGACAGTGGGAGCCGCGTCCGCGAGCGGTCAGGTGGCGGTGCTGCACGC83820CATCGAGGCGGTGCGCTCGGCCCGCGTCGACGTTTGTATTGCMTCGGCGGGCTGATGGA83880TCTCTCGTACTGGGAGTGCCMGCATTCAGTTCGCTCGGCGCGATGGCGCCCGGACATGA83940CGGGATACAGGCGGCGCTGGTCAGCCGGCCGTTCGATGCGAAGCGAMCGGATTCGTCTA84000TGGCGAGGCCTGCGCGGCGCTCGTGATCGAGCGCGACGACGCGCGACATCGGMCGGCGT84060TGCACCCTATGGATATATCGCAGGCTGGAGTACGGTATTGGATGCCMTCGCMTCCCGA84120CCCATCGTTCGCGGGCGAGACCGCCGTGATCCGCGACGTGCTCGCGCGCGCCCGCCTCGA84180CGCGCGCGATATCGACTACGTGMTCCGCACGGTACCGGCTCGCCACTCGGTGACGTGAT84240CGMTTGCAGGCGCTGCGCGCATGCGGGCTCGACCACGCGTATCTCMCGCGACCMGTC84300GATCACCGGCCACGGTCTTTCGGCAGCCGGCGCGGTCGMCTGGTCGCTGTGTTGTTGCA84360GATGCGTGCGGGGCGCTTGCACCCGACACGTAATCTCMTCATCCGATCGAGTCGGATTT84420CMCTGGGTGCGCCAGCAGTCGGTGCCGCACGAMTCCGGCATGCGCTGAACCTCAGCAT84480CGGCTTCGGCGGCGTCMTACCGCCGTATGCGTGAGTCGCGACGATGCGGCTGCCGCCGG84540CGCTTTATAGGAGTCGATACGATGCAGGTATTCATGTTTCCGGGACAGGGTTCGCMGCG84600[1508]AMGGCATGGGCGGTGCACTGTTCMGCGATTCGGAAMTTGACCGACGACGCGAGCACC84660ATGCTCGGCTATTCGATTCGAAMCTTTGTCTCGACGATCCGCGCGACGAGCTGMGGAT84720ACGCGTTTCACGCMCCGGCGCTGTATGTCGTGMTGCGCTCGCGTATTACGCTCGMTT84780GAGGMGCGGCAAMCCGCCAGATTTCGTGATGGGGCACAGCCTTGGGGAATTCMTGCG84840TTACTCGCCGCCGGGTGCTTTGATTTCCTGACGGGACTCGAACTGGTGCGGTTCCGCGGA84900CMCTGATGGGGCGCGCTGAGMTGGTGCGATGGCCGCGATCGTCMTGCGMTCAGGCT84960ACGATCGAGAGMCCCTCATCGAGMCGGCCTGACGCAGCTGAGTCTCGCGMCTACAAC85020ACGCCGTCGCAMTCGTGATCTCGGGTGCGGCCGACGAGATGGCGMGGCTCMCTCCTG85080TTCMTTCTGGCGACATGCGCTGCTACCCCCTCMTACGAGCGGCGCGTTTCACTCCCCC85140TTCATGCGATCGGCCCGCGAGGAGTTCGCACGTTTTCTGCAATCGTTTCGTTTCGCGGCG85200CCGAGCGTGCCGGTGATCTCGMCGTGTCAGCGCGTCCCTATGMTCGGCCGGTATCGAT85260ACGGCGCTCGCCAGTCAGATCGATCGCCCCGTACGCTGGTGTGACAGTATTCMTATCTG85320CTTGGCGTCGCCGCGCGACGTGGCGMCAGATCGAGTTTGAGGMCTCGGCMTGGCGAC85380GTGCTGACGCGTCTCGTGCAGGCCATTCGCCAGCAMCATCGCCGGMGTGATCGCCCAT85440ATCGCTACCGAGGTAGAGACGGGTTCMCGGTAGCGCCGATGGGTGCCGTGGCGGCGGAT85500GCGCGMGTGCGGCGGATMGGTGGCGGCGTGGMTCGTCGCTATCCGGTCGGCACGCGC85560GTGACCTCGAAGCACCCCGAGTACCGGGATCTCGTCACGCGMCGGMGCGGTAGTGCTG85620TTCMTCMCGCGCGGCGGTCTACATGGMGGCTATCACGGCTATTTCGATCTCMCGAG85680CTGACGGCTGCCTGMTTCGCGCGTCGCAGCCGCCTGCCGCGCGCACTCCGCCGGCCTTG85740TTGCTCGGGGCGGGCACTGCGCMGMCAGCACGCCCCMACGTAAMTCCGCACGTTGT85800ACCGGATATTCAGTGCATACGTATCGCTCTTCMGGGATATTGTTAGTAGTCCGTCMAG85860TTGTGGGGATGAGATGGTATCGMTGGTGACGATGCCGGGGTGACGCTTTTGGMGCATG85920CTTGATGATCCGATGCTTCTCATACACGTGACACGTCGCACTCTTGAGCACTGTTTTTGA85980CCATCCTCTCMGGTCAGAGCCGAMTCCAACCTACTGGAATCATTCCATGACAGCGCTT86040CTAGATCMCTCAGATCCGCAGATGACTTTCAGATAGAGCTTCTTTTTCCCGAGCAGGM86100TTTTTCCTTCGCATTCMGCGACCTCACGCTTGCCTGCGGTCGMTGGGTGACGCGGCAC86160CGTCAGATGTTACGTGMGCGCTTCTGCGATATGGCGTCGTTCTGATCCGCGGCTTGGAT86220TGCGACCGMAGGCATTCGGTACTATCGCGGAACGACTCGAACCGGGCGAGTTCGATTAC86280ACCGCCGGGAGCGGGCCGCACACCTCTGTCGACGCAMTGTTTTCACCATAGACGTACCC86340GGCTCGATGGCGCTGCCGCAGCATMTGAGATGGCTTATAACCTTTACTGGCCMTGCAT86400GTTCTCTTCTTCTGCGAGCAGCCTCCGGCCCCGGGTACGGGTGGMCGACATCGGTATGT86460GACGCTCGCCMTTCCTACGGGACATGMCCCGACGATCTTGGATCCATTCTCGMGTTT86520GGCATCCAGTATGTACGGMTTTTCCTAMMTATGCCGTACAMTCTATCGGGGATACG86580TTTGGGACGAGCGACCGGGATAMGTGMCGCGATATGCGCGGAGCGGMMTCGAGCCA86640ATCTGGATCAACGATGACCATCTGCAGATTCGTCAGTATGCCACGGCGGTCAGACGCCAT86700CCTGTTACCGCGGAGGAMGTTTTTTTAGCTCGGTTTGCGTCTGTCATCCAGCGGGCTGG86760TGGGATTTGCTCAGGCGTGCGTACCCGGCTGCATCGCTTCCTCGCTCGCAAGATGAMTC86820TGGCAGACGGCTTTGTACGGAMTGGCGATCCGATACCGGATGATGTMTACATCATTTG86880CTGCATGCTTACGAGCACCGGGMTACCATGTMTGTGGGAMGATCAGACATTCTCTAT86940[1547]ATCGACAACATGCGCGCATCACATGGTCGCCGCGCATGTACAGGTCCACGTGCGATTCTC87000GGCTCTTTCCGCACGTCCATGAGCATGCGCCAGCTGGAGGCGGGCCATTCATMCTCGTG87060GATATTCGGCMGCCCTTGGCCGCGTCGCGGATGATTTGATCMGCGCCCACGCGCACAT87120GAAAMTACACTGACGGGCGCGCATCCMTCACTTGMCAGGAMGTCATTTTCMTGM87180TCCAGCCATTCATTTTTCCATTCTGTCGCCGGTGGGCTTTTTAGACCATTTTATTGGCM87240TGCCCTGGCGATGCTCMGAGCCCCGCCGTTCMTGMTTTTATGATGCGAGTGAGTCAT87300GAGTGATCTGACGGGGTCCTGGTCGCTGGAAGAGAGCGMTTCAGGCTGGCGMCGGCM87360CATTACTTACCCGCTGGGGAGAMTCCATCGGGACGCATCATCTACATGGAMGCGGGCA87420TATGTCCGTACTTTTGATCGCCMGGGCAGGGCGMTGTTGGATTCATTCCTGAAAMTT87480CTGGCTAMTATATTTGGCATTMMGMTMTTGGATTAATTAGGTTGATCCGGGCAM87540TTCCGGAGTCCTGGGCTATTCCGGMGATATTCMCGGATGGGAAMCGGTTTTACACCA87600CGTTGATTTATCMGTTACCCGGATTTCGTTGGGACMGACTTGTGAGGGAGGTMGGTA87660CGMGAGGGGCGGCTCGTCCTCGAGMTTCMCTGATTCCGGTTCGAGCCGGCTGGTTTG87720GGCGCGAGAGATTTMMCGACAGCGGGTTGGGATCGCGGGATTCTGCMCGGTTMTAT87780TTCTTGATGGGTTGTTGGTTTTTGAMTTGCGAAMCATGGAGTTCTTMATGAGCGACG87840CCGGAMTGTCGAGMTGATGGCAAGAGCATTAMGGMTTGCTGTAGATCCCGMGTGT87900TGTCMTCATCAAAMCCCGGATCGACCTGMGCATTCCCGTCCATAGCCAMGCCCCCA87960ACMCATCCCTGGGGTGAGTGGATTTATTGCGGGCATGMATCTCTTTTTGCATATMGC88020GAGMGGGTTGCAGTTCTTTGCMATCAGGCAAGCATTTATGGCMGGTGTTCCGCAGCC88080MCTCGGCCCATACTCCATTGTCGGGGTGACTGATCATGAAGTCATATCGCAMTCCTGA88140AAMTGGCGATCGATGTTGGTCGACCGCACTGGCGGTAGAGAGGGTMCACGAGGGCTTA88200GTTTTGATATCGATCTGMGMGAAGAGTCGATTGATGTCATTGGATTTTGATGCTCATC88260GGCGMTGCGTMTTTGGTTCAGCCGGCTTTCAGCGATCGCGCACTCGCGAGCTATTTGA88320CCGTCATCCATCATGAATTCGTTCACMTATCGAGGCTTGGCCTCGTGACGGGGCAGTGT88380CATTCCGAGTGGMGCCCGGAAMTTTTTTCCCAGCTTGCAGCGCGTCTCTTCTTCGGTA88440TTGGAGACCCAMGGAGGCCGCACGTCTCGMGGGGCCACTCGTGATTACTGGGGTGCGA88500TTCCTGTTGTCTTGMGMTCGACACCTCAGCGMCGATGGAMCGAGGCGTGGACGGGG88560TTGCGACACTTAGCAGCATTATGTACGACTTGATTCCGMACGCCGTGACGMGGCGGGG88620TGGATTTGTTCAGTCAACTGTGCAAGTCGAGGGACGATACGGGGGGACTGGATGACGACC88680MCTTGTCGATTTGATGCTCMTATTATCTTTGCCGCGTTCGACACTACGACMTGGGM88740TMCCAGCATGGCCTATCTGCTTGCCMGTATCCTCATTGGCAGGAGGTCCTGCGAGCAG88800MGCCATGCGCCTACCTGAGCMGCGACGTTGGCCGACTTCMTGGCATGGAGCGMCTA88860CCCAGGTCTGGMGGAMCACTTCGCATGTTTCCCGTATCGGCCATCACACCCCGCGTTT88920CATTGGGTGAATGTCAAGTTGGCTCCTACGTCGCTCCTCCGCGGACGTTGGTCTTCMCT88980TCACCGGTCTCCTTGGCCAGGATCCGGATCTCTGGGATCATCCACAGCGCTTTGATCCCG89040AGCGATTCTCATCGGCGCGCGCGGAGCATCMCGGCACCGTGGCCMTATCTCCCGTTCG89100GACACGGTGTACATACTTGTGTCGGCGCCGCCTTGGCAMTCTTGAGGCAAGAGTCTTTT89160GGAGCATCGTCTTGACTCGGTATAGGTTTAGCCTTGCCMGGATTACGATGCGGTTCATT89220GCATGAGCCCTTTGGGGAGCGTCTCCGGTGMGTGGCGTTGMGGTCGMAGATTGTGAC89280[1586]GGTACTGMGCMGCGGAGCTGGGMTGCCGGTTGTCGACTTGATTCGTCAGGCCGGCAT89340TTCGGMCTGACGTTCTGTCMTCGMGMGTMTGCGCTGGGATGCAGTCGGATTATTG89400GCAGGAGACCGATTGCGGCCGCMTTCTCAMCGGGTGTTGTCATTCTGAGGGMTTGTA89460MTGMTCGTCTTGTACATTCTGCGCCMTTGTTGAGCTTCACAMTTTTACTAGCGATG89520GTCACGAMCCCGCTAGCGGCGTGCACGMAGGAGCCAGCAGCGCTTGCGACCTCMTCT89580CACGCTCGGTCMTGTAGMGAGAGGGGCAACCGCATCGACAMCTGCTGCCGTGGMCG89640TGGCCTCATCGTTGTCGCCTTCGGCTCGCGCGCMCCTGTCCGATAGMTCGCTCATCTC89700CTGCGTCMCMGCGGCGCTACAGTATGACMGGGCGCCTACMTTTCCCTTTCGCTCAG89760CTTCTTCAGTCATGACTATCGCTATCGMTCGCCAGATCAACCAGATGTGATTGCTCTGA89820TTGCAGAGCTGGACGCCTATCMGACTCCCTGTACCCACCTGAGAGCAGACATATCCTTG89880ACCTCGCGGCGCTGMGCMTCGAATGTCCTGTCTGCAGTTGCGAGAGACAGCGCGGGTA89940ATGCCATTGGCTGTGGAGCGATCGTTCTCTACCCTGAGTTTGGCGMCTCAMCGCATGT90000ATGTTAGTCCTCGCGGTCGCGGGCMGGCGTTGCCMGAGACTCTTGGCTCTTCTCGAGT90060CGMGGCCATAGGTCTGGGCTGCAAGTTGCTCMGCTCGAAACCGGACCGTACCMCACG90120AGGCGCTAGCTTTGTATGCCTCCGCCGGCTACGAGCGCCGCGGCCCGTTTGGCGATTACA90180CGMTGATCCACTGAGTGTATTCATGCAGAAGCGCATCATCGCCGCGTAGGGCTCGCTTC90240GTGTCGCGTCTATCCCGTAGCCTCAGCGTAAGCGGTCGGTAMGGACGCCTCTCCCCTCC90300ATCTGCGTCACACTCGGTTAAGATCGACTTGCCGTGAGCGGCGAGTTTGCTCAGCGGCAG90360ATCGTCGCCGCTACCCAGCGGACTCTTCGCCCATGCCATCAACTGCTTTCGMGCGCACC90420TCGTTGCCCTTGCGCGAGAGTCTTCATGGTTTTGGCCTGCACTGGTTGCAGTCCGGTCCC90480TGMTCTACCTTCCTGGTGCATCGGTGCTGGTGCCGTACGGMCCTCGTCTGGGACTCTT90540TGCATGGATTGGAGACATCGTCAGCATTGCGTGACGTCGATGTTGCGTACTTCGATGGCG90600CTGATCTTTCTGCCGCGCGCGACGCMCGTTTCAGTCTCATTCMGCAGGATGCACCCCC90660MTTGCCATGGGAGGTCACCMTCMGCCGCCGTTCATACCTGGTTCGAGGCGGCTTTGG90720GTTACGCCGTGCCACCATTGMGTCGCTGTAGGCCGCCGTCGCGTCGCGGCCAGAGTATG90780CMCCGCAGTCGGGTTGACTCTACTTCCTGATGACACGATCMGGTTCTTGCACCGTATC90840GGGCACGTGTTGACGCGMTTCTCGATCGTGTGGCATTGCTTCGCGGCTTGCCGMTGCC90900ATTCGGACCGACMCGGCMAGAGTACTGCGGTCGGGCGATGTTGACCAGGGCATGCCAG90960CGAGGCGTGAACCCGAAGATCCTTGTAGGTGTTTCCGGGGGCTGTTCMCTGGCCGGTCC91020ACTCGGTGCCMGAMCTTGTTGCATGAMATCTMCGGTAGGAGTCTCCTTTCCTATAC91080TGGATTCACCTGACGAMCGGGAGGCAGMCATGAGCATCCGCGTTGCCGCCMTGAGCC91140TTCMGCACTATCCMCTGGTCACCMGGGCTATGCCMGCTGCTGCTGACGGGCTTCGC91200GCTGATACCGCTGTATTTGATTGCCTACCTATATTTCTTTCAGGATCCTTCGCTGAMTT91260CGAGMTCACGCTTTTCACGAGCTTGCGATCGCTGCCGCCACGCTCGMGGCGGATTCGT91320CACCTATGTCTGCTGGCGTTGCTATTGTCTGTCAGGCGMCCGCTGCTGCGTTGGCTCAC91380GCTCGGCTTTCTCGGCTTCTCTCTCATCTATGCGTTGCATGGCGTCTTCACCGGCATGGC91440ACATCACMTATCTGGCTGTTTCTTTTGTACGGGCCGGCCTCCCGCCTAGTCATGTCGAT91500ACTTATTTTCATCGGGCAGCTCACTTATAGCCACGCGGCCGACAGCGCMCCAMCGTGC91560TGATCGTCGCGTCTGGGCGACGTGGATTGCACTGTTTGTGCTGATCGACCTCTCGGTTGC91620[1625]CGGMTCGCCTACTCGCCAGTGGCCGGCGCCCCATGGATGCGCGTTTCGATGGAGGGAGG91680CGCGCTCTTGCTTTCCACCGTAMCGTCGTTGCGCTCGTCCAGCGTCGCATTCGCTCTCC91740GCTGATGACGATCTACGGCATTTCGGTGACGTCGTTCGCACTTTCCTCTCTCGCATTCAT91800CCTTGGGGTACCGTGGMTCATATGTGGTGGCTTGCGCACGCMTCTTTGCGGCCGGCTT91860CTTTCTGCTCAGTTTCGGGGTTGTTCAGGCTTTTCACACGACGCGCTCGTTTTCGMGAT91920CTATAGCCMGGAGAGCTGTTTGCCCGGTTGCGAGMGCGATGGGGCGCACGGAGAGGGC91980GCTGCAGGMCTGCAGCGGACCMCGAGMGCTCGAGCATATGGCGGCMCGGATCCGCT92040TACAGGCGCGGCCMTCGGCGCCAATTCATCGATCGCGTGGAGGCCGAMTTGCCCGGGC92100GAMCACMTGGCGCGCCGTTCTCGTTGCTAGCGCTCGATCTCGACCACTTCAAMTTAT92160CMTGACAGCTACGGGCATCAGGCGGGCGACCGCGTGTTGCAGCGCTTTGTTACGAMTG92220CCGTGATGCGATCCGGCCATTCGACGGCGTCGCGCGGGTAGGTGGAGAGGMTTCATGGT92280GCTTCTGCCGCMGCTGCTCTGGACACCGCTCGCTCMTTGGTGAGCGTATTCGCGCAGC92340GATCGCTGGCACACCGTTTGAGGCCGACATCGGTCAGCCGATTGAGGTCACCGTGAGCAT92400TGGCGTTTCCGAGTTCGGGCGTGATGGGGAGACGATCGACGCGATCCTGCGCMGGCGGA92460CGMCGCCTATATCACGCGAAGCACCMGGTCGCMCAGGGTGGTCGCTGCGTAGTCCGC92520TGCGGGTGTTCTGACCGGCCCTGAAGTTTGCCTCAGGATGCGCTTACGAGCCGCGCMTG92580CGGTAGGGTTCGGCTTTMCTTACTGACCAAGGTTGTCCATGMCAMCAGGMCTCATT92640GACGCAGTCGCTGCTAGCACCGGCGCCAGCMGACTGCCACTAGCGMGTMTCCMGCC92700CTGCTGGAGGTCATTACGCACGCGGTGAGTGCCGACGATACCGTACMCTCATCGGCTTC92760GGGGCGTTCTCGCAGGGGCAACGCGCAGCCCGTATCGGCCGCMTCCCACMCAGGCGCG92820GAGATCGCGATCGCCGCGGCGAMACGGTAMGTTCATCGCAGGCMGGCATTTMGGAT92880GCGGTGAACGCGCCGTGACGMGCATGACGTTGTGCMGCTGCCTATGTGGGTGTGCACC92940GTGAGGTCGTGGACGTTGTGGTATCCACGCGCGCMCGGCTGGCCGTCGTAGCGATGCAG93000GTACGCGMCACGGCGTCGAGGTCGTGCATGGCCGAGATGTACGGGAGCGCCGGCGCGCG93060ATTCGTTCCGGCCGAGCCGTCCAGGTCCTTCGGCATCGCCACGGTTGGTCGCTGCGCAGC93120GGGACGCGTGCCTCCGGTGCAGCGACGMTCGATCGATGCTGTCGACGTCGAGCGTGACC93180GTGTCGCCATCGTCTCGGCGTGCCGGCGCAGCCACGCGACCACGGTGCGGGCCCTGCCGA93240CGCCGATGCGCGGCACCGACCGCCACCAGGATCCGCGGCGGCGGTTGCAGCTGTCCMCG93300GTGCGCACCTCGATCTCGCAGTTGCTGGGCMCGAGCGCGGMCCMCGCGCGATCGCGT93360CATCGGGCGCCGGCGCCGCCTGGGCCTAGTTGTGMGCGTCMGAGGTTGTTTCTCGATA93420CGGGMGTCTGGCCATCGGCGCMCGCCGCCGATGGCGCTATGAGCACGATGCCAGTTGT93480AGTGATGCTGCCMCTCGCCAGGGCTTCGACGCGATGGGCTGAGCTTTGGTACGTCCATG93540CGTAGGCCCACTCACGTMCGCTGACTGGATGMGCGTTCGGCCTTGCCGTTGGTCTGAG93600GACGGTACGCCCGTGTAMTTTGTGCCGGATCTCCAGTTCCTGACAGGTCCGAGCAMCT93660CATGTGAACGGMCGCCGMCCGTTGTCGGTTAGCMTCGGCGCACACGCACGCCMGTC93720CAGCGTACCAAGCCACGGCATCGCGCAGGAATTTCACGGCACTCCCTTTCGTCTCGTCTG93780GATGCATTGCTGTGTAGGCAATCCGGGCATGGTCGTCCACGGCGACGMCAGATACTCCC93840AGCCMCACCATCGACCGTATCGCGCCGATTGCCGGTCACACGATGGCCAGGACGCGCGA93900TACGGCCGAGCTTCTTGATGTCGATGTGCAGCMGTCGCCCGGTGCCTCGTGTTCGTAAC93960[1664]GCTGMCCGGCTCTCTTGGTTGCAGGTCGCTCMCCGGGACAGTCCGGCACGCGCCAGCA94020CCCGACTGACGGTCGAGGCCGACACGCCCACCTGTCGAGCGATCTGACGTTGCAGCMGC94080GCTGCTGGCGCMCTCCACGATCAACMGGCAGTCGCCGGGTCMTGGAGCGTGGAGAGC94140GAGCCGGTCTCGATGAGGCGTCTGCCAGCGCGAGTGCGCCGCCGACCAGATAGCGCCCM94200GCCATTTGCGTACCGTCGGTGCTGTCACGCCATGATCGACTGCGGCTTGCGGCACGCTCG94260MCCGGATTCGGTGATCTCCTGMCCATCTCAAGTCGACGCGCGAMGTGAGTCGGGCAT94320TCTTATGGGTGTTCATCCGGCTGGGTTCCTGGMGGTTGGGTGTTTGGCGACTTCCAGTC94380TCCCAGACTCAGTCCGGATGMCCATCGATACMCCTATTGMGCTTCACAGCTAGCGCG94440GCATGCGTCGGCGCGGTAGTCGCTCAMCGGCAGCCGGGATTCCCATTCGMCMTGTTG94500TCCGATGCTGACTGACATAGGACAGCAGGCTGTCCATGTTTCGAGCCTTGTCGAGGGCGG94560TTGCTGGCMCTGCTCCCGGGCGACGTGATGGACTGCGCATTCACCGGGACCATCGAGGA94620GCCAGGCGGAACGCCAGTAGGGTGACTCMTCTACATGCCGCMCCGAMCCGATACTGT94680TCAGCMCCTTAGTGCGACGCTTTGTCCGGGATTACCGGGGCACCTCCACTGMGTTGCC94740CAGCACCAGCATGTCGCCGGGCGCGGCGGACTTGGTGMCGTGATGTAGTTGTTGGTCGG94800GMCMCAGGTTCTTGATCGCGMCAGCGACACTGCGGCAAMCTGACGTCCAGCTGCTT94860CACGATCTGTTTCGGCACTTGGTCCCGGATCTTCTCGTTGATTCTGTACTGTAGGTCAGG94920TGTGTTCGATGGACCGCCCCCCTCGGCGCCACCAGTCACCGTCACCGCCTGACTCTCCAT94980GACACATTTGATGGTCTGATCGCGTCCACTACCCTCGACCTTCACCTGCGCCATCGCATA95040CACGCTGGMGTGGCTTCGTTGGTGMCGTCTTCCCAGGGATGGTTCCGATGAMGCTGA95100GGAGCTGACGTATGTATTGATCGCCATCGATTGAGCCTTGGTTCCGGACAGCAGCATCTG95160GCCGTCTGGTCGAGGCGTCAAGGTCATGCCCGCCMGTTCCAGTTGATCGTGTCGTTTGG95220MGGGCATAGGTCGTCACGGTGACGCTGATGCCGGTCGGATCCGGCCCGGGAGACACCTG95280GGTGTCGGTCACGCTCCCCAGCTTGACATCTGTGGCGMGACAGGGGCATTGGTGMCTC95340GCACCACGACGCTTTCACCGACTCGGGGCCCTTTCGACCCACCAGACTCCAGCCGGATGC95400ACGCCTGTCAAGAGATTGGGGAAGGT95426<210>2<211>294<212>PRT<213>Psuedomonassp.NO.2663<400>1MetLeuLyslieHisGlnLeuAsnArgLeulieAspliePheSerHis151015AlaLysPheGluGlulieTrpMetValSerValPheThrLysLeuGly202530ArgVallieSerAsnlieGlyThrGluArgPheAlaGluAlaLeuHis354045SerLeuPheValThrSerValAsnValGlySerThrLyslieAlaVal505560[1702]TrpPhelieAsnGluArgGluGluGlulieValGlyMetHisProLeu[1703]65707580GlyGluPheSerAlaAspGlyArgSerAsplieAspValGlyLeuLeu859095GlnLysSerValPheAspAlaValAspSerArgLeuMetGluArgVal100105110LeulieAlaArgGluLeuHisLeulieHisPheGlyThrSerAlaThr115120125ProGlnThrGluTrpGlnArgLeuArgSerCysGlySerAspGlyGly130135140PheGlnValHisLeuValSerArgLysLeuAsnArgArgTyrLeulie145150155160SerLeuTyrArgThrCysGlyThrProAspTyrThrlieGlnGluMet165170175SerLeuLeuLysSerTyrAlaGluAlaLeuMetProlieValGlulie180185190HisAlaSerHisArgLeuCysGluLeuAlaArgArgProMetGlnVal195200205SerGlyAspAlaGluSerSerlieGlyHisLeuThrValArgArgLys210215220PheGluSerArgLeuArgSerAlaAlalieThrLeuSerAspArgGlu225230235240lieGluValCysAlaGlyLeuLeuSerGlySerThrPheArgGluLeu245250255AlaAspGluLeuGlyValLysSerSerThrlieGluThrTyrlieLys260265270ArgAlaAlaThrLysLeuGlyPheLysGlyArgHisGlyLeuValLys275280285TrpAlaLeuSerGluSer290<210>3<211>360<212>PRT<213>Psuedomonassp.NO.2663<400>1ValArgGluArgArgProThrLeuLeuLeulieValLeuValSerGly151015TrpAlaGlyGlyArgHisGlnAlaAsnlieMetThrLeuArgLysArg202530MetAspAspArgGlulieValPheMetTyrProGlyGlnGlyValGln[1742]354045TyrPheGlnMetGlyLysSerLeuPheGluHisAspArgValPheArg505560AspSerLeuMetSerLeuAspArgMetValThrValGlulieGlyThr65707580SerLeulieAspTyrValPheAspAlaGlyArgAspValSerSerPro859095[1749]PheAspMetThrLeuTyrSerAsnGlnAlaValPheCysPheGluTyr100105110AlaMetThrGlnAlaLeulieSerHisGlyValTyrProAspHisLeu115120125LeuGlyAlaSerLeuGlyGluLeuValCysAlaSerValSerGlyAla130135140LeuSerProArgAspCyslieAlaLeuLeuAlaAspSerAlaArgVal145150155160MetAlaArgHisCysAlaProGlyGlyMetLeuAlaValLeuAlaThr165170175ProAspLeuPheGluArgAspAlaArgLeuArgGluAlaGlyGlulie180185190AlaSerlieProSerAlaGlnHisPheValMetAlaGlyThrArgAla195200205GlylieAspMetAlaGluAlaHisLeuSerValLeuGlyGluThrThr210215220MetArgLeuProValGluTyrGlyPheHisSerSerAlalieAspPro225230235240AlaArgAlaProLeuArgLeuCysPheGlnSerValAlaSerSerArg245250255ProValLeuProAlaTrpSerCysArgThrGlyArgProValGluArg260265270TyrAspAlaGluHisPheAspThrValLeuArgAspVallieAspPhe275280285LysArgThrlieGluTyrMetGluArgArgSerAlaCysValTyrLeu290295300AsplieSerProSerGlyThrLeuSerThrGlyCysArgThrLeuLeu305310315320GluGluAlaSerArgSerGluThrPheAlaValCysThrProPheSer325330335ArgHisProThrLeuAspProValHisTrpMetArgProThrProVal340345350[1781]SerValMetArgMetArgThrAla355360<210>4211>296<212>PRT<213>Psuedomonassp.NO.2663<400>1MetArgLysValPheLeuPheProGlyGlnGlySerGlnArgArgGly151015MetGlyArgGluLeuPheGlyAlaTyrProAlaLeuThrAlaCysAla202530SerAlaLeuLeuGlyTyrAsplieAlaGluLeuCysLeuAspAspPro354045ArgArgGluLeuGlyGlnThrArgPheThrGlnProAlaLeuTyrVal505560ValSerCysLeuSerTyrLeuAsnArglieAspSerGlyGluArgSer65707580ProAspValAlaValGlyHisSerLeuGlyGluPheAlaAlaLeuPhe859095AlaAlaGlyAlaPheAspPheGluThrGlyLeuAlalieValGluLys100105110ArgAlaAlaLeuMetSerAspAlaProAspGlyAlaMetValAlaVal115120125LeuGlylieAspGluGlnArgValArgGlulieLeuAspHisHisLeu130135140AlaGlulieGlnAspAsnAlaSerSerProGluValGlulieAlaAsn145150155160LeuAsnHisHisLeuGlnCysVallieSerGlyThrArgGluArgLeu165170175ThrGluProThrLeuAlaArgTrpPheGluAspAlaGlyAlaSerPhe180185190lieProLeuAspValGlyGlyAlaPheHisSerValArgMetLysGly195200205ProAlaAlaArgPheAlaAspPheLeuLeuSerValGluLeuArgPro210215220LeuArgMetGlnValValSerAsnTrpThrAlaHisAlaTyrProAla225230235240GlyAspTyrArgProMetMetValAsnGlnMetPheAsnProValArg[1819]245250255[1820]TrpArgAspSerlieAlaPheLeuLeuAlaArgGlyTyrArgAspPhe260265270HisGluValGluProGlyAspValLeuSerArgLeuGlnArgHislie275280285GluSerGluGlnProHisGlyMet290295<210>5<211>429<212>PRT<213>Psuedomonassp.NO.2663<400>1MetAsnPheGlnArgArgArgGluLysAsnValAsnThrValGlylie151015GluAlaMetAsnLeuTyrAlaGlyValAlaSerLeuAspValThrAla202530LeuAlaArgHisArgGlyLeuAspMetArgArgPheAsnAsnLeuMet354045MetLysGluLysThrValAlaLeuProTyrGluAspProValThrPhe505560AlaValAsnAlaAlaMetProlieValAspAlaLeuThrGlnSerGlu65707580ArgAspGlnlieArgMetVallieThrCysThrGluSerGlyPheAsp859095PheSerLysSerMetSerThrTyrCysHisAspLeuLeuGlyLeuSer100105110ArgHisCysArgMetPheGlulieLysGlyAlaCysTyrSerGlyMet115120125AlaGlyLeuGlnMetAlaValAsnPheValLeuSerArgThrTyrPro130135140GlyGlyLysValLeuValLeuAlaSerAspValSerArgPheMetVal145150155160GluLysGlyGlyAspAlaLeuSerAlaAspTrpSerPheSerGluPro165170175SerGlyGlySerGlyAlaValAlaMetLeuValSerAspGlnProHis180185190ValPheGlnlieAspAlaGlyAlaAsnGlyCysTyrGlyTyrGluVal195200205[1857]MetAspThrCysArgProAlaAlaAspAlaAspAlaGlyAsnAlaAsp210215220[1859]LeuSerLeuLeuAlaTyrLeuAspCysCysGluAsnAlaPheLeuAsp225230235240[1861]TyrArgLysArgValAlaAspAlaAspPheAlaThrThrPheSerTyr245250255LeuAlaPheHisThrProPheGlyGlyMetlieLysGlyAlaHisArg260265270ThrMetMetArgLysLeuValMetAlaLysProProGlulieGluAla275280285AspPheAspGlnArgValAlaProGlyLeuAlaAspCysGlnSerVal290295300GlyAsnlieMetGlyAlaThrAlaAlaLeuSerLeuAlaSerThrlie305310315320ArgHisGlyAspPheSerGluProArgArgValGlyValPheSerTyr325330335GlySerGlyCysCysSerGluPhePheSerGlyValAlaThrHisGlu340345350GlyThrHisArgLeuAlaGluLeuGlulieGlyGluGlnLeuAspArg355360365ArgArgAspLeuThrMetAlaGluTyrAspAspValLeuGluArgSer370375380ArgGlulieArgPheGlyThrArgAsnLeuAspValAspThrAlaLeu385390395400LeuAlaSerAlaArgGlyValHisArgArgProThrLeuPheLeuLys405410415SerlieArgAspPheHisArgGluTyrGluTrpValAsn420425<210>6<210>10<211>251<212>PRT<213>Psuedomonassp.NO.2663<400>1LeuThrValGlnPheAspAspGlylieCysArglieArglieAspArg151015ProAspThrGlyAsnAlalieAspAlaArgLeuValAspGluMetAsp202530SerValLeuAspGluTyrGluAlaSerThrArglieValVallieGlu354045GlyLeuProAspLysPheCysValGlyAlaAspPheMetGlnLeuAsp[1898]505560AlaArgLeuThrThrGlyAspSerLeuLysAlaProAspProAlaPro65707580LeuTyrAspLeuTrpThrArgLeuAlaThrGlyProTyrValSerlie859095AlaHisValGlnGlyArgAlaAsnAlaGlyGlyValGlyPheAlaAla100105110AlaCysAspValValValAlaAlaGlnThrSerSerPheSerLeuSer115120125GluLeuLeuPheGlyLeuMetProAlaCysValLeuProPheLeulie130135140ArgArgValGlyPheAlaArgAlaHisTyrMetThrLeuThrThrGln145150155160ProlieGlyAlaAlaGlnAlaArgGluTrpGlyLeuValAspValCys[1912]165170175GlyAlaAspSerArgAspLeuLeuArgArgAspLeuLeuArgLeuArg180185190HisLeuLysLysAspAlalieThrArgTyrLysArgTyrMetAlaSer195200205LeuAspAspSerLeuAlaMetSerArgAlaProAlaValAlaAlaAsn210215220LeuGluValPheGlyAspAlaAspAsnLeuGlnGlylieSerArgTyr225230235240ValAsnAlaGlyLysLeuProTrpGluProAsp245250<210>7<211>79<212>PRT<213>Psuedomonassp.NO.2663<400>1MetSerGlnGlnGlnlieLeuGluLeulieAlaLysHisThrArgGlu151015lieValProGlyLeuGluAspHisValPheAlaProGlyAspSerLeu202530SerAsnLeuGlyAlaAsnSerValAspArgAlaGlulielieValMet354045ThrLeuGluValLeuAlaArgGlulieProlieAlaLysMetAlaAsn505560AlaGlnAsnlieGlyGluLeuAlaSerlielieAspAlaAsnAla[1937]657075<210>8<211>421<212>PRT<213>Psuedomonassp.NO.2663<400>1MetArgAspAspAlaAlalieValValSerGlyValGlyValValSer151015AlalieGlyGlnGlyArgAlaAlaPhelieAspAlaLeuLeuGluGly202530ArgHisArgPheAspThrMetArgArgProGlyArgGlnTrpArgPro354045AsnGlyAspValProAlaThrAlaPheLeuGlyAlaGlulieAspAla505560LeuThrlieProAlaThrValSerArgGlyLeuSerArgThrAlaSer65707580LeuSerAlaGlnAlaAlaLeuAlaValLeuAspGluAlaTrpArgAsp859095AlaCysLeuGlyAspValAspProGluArglieGlyLeuVallieGly100105110GlySerAsnMetGlnGlnArgGluGlnValLeuLeuGlnAspAlaTyr115120125ArgGluArgAlaGlyPheMetArgProThrTyrAlaMetSerPheMet130135140AspThrAspMetCysGlylieCysSerGluAlaPheGlylieArgGly145150155160PheAlaGlnThrValGlyAlaAlaSerAlaSerGlyGlnValAlaVal165170175LeuHisAlalieGluAlaValArgSerAlaArgValAspValCyslie180185190AlalieGlyGlyLeuMetAspLeuSerTyrTrpGluCysGlnAlaPhe195200205SerSerLeuGlyAlaMetAlaProGlyHisAspGlylieGlnAlaAla210215220LeuValSerArgProPheAspAlaLysArgAsnGlyPheValTyrGly225230235240GluAlaCysAlaAlaLeuVallieGluArgAspAspAlaArgHisArg245250255AsnGlyValAlaProTyrGlyTyrlieAlaGlyTrpSerThrValLeu[1976]260265270AspAlaAsnArgAsnProAspProSerPheAlaGlyGluThrAlaVal275280285lieArgAspValLeuAlaArgAlaArgLeuAspAlaArgAsplieAsp290295300TyrValAsnProHisGlyThrGlySerProLeuGlyAspVallieGlu305310315320LeuGlnAlaLeuArgAlaCysGlyLeuAspHisAlaTyrLeuAsnAla325330335ThrLysSerlieThrGlyHisGlyLeuSerAlaAlaGlyAlaValGlu340345350LeuValAlaValLeuLeuGlnMetArgAlaGlyArgLeuHisProThr355360365ArgAsnLeuAsnHisProlieGluSerAspPheAsnTrpValArgGln370375380GlnSerValProHisGlulieArgHisAlaLeuAsnLeuSerlieGly385390395400PheGlyGlyValAsnThrAlaValCysValSerArgAspAspAlaAla405410415AlaAlaGlyAlaLeu420<210>9<211>377<212>PRT<213>Psuedomonassp.NO.2663<400>1MetGlnValPheMetPheProGlyGlnGlySerGlnAlaLysGlyMet151015GlyGlyAlaLeuPheLysArgPheGlyLysLeuThrAspAspAlaSer202530ThrMetLeuGlyTyrSerlieArgLysLeuCysLeuAspAspProArg354045AspGluLeuLysAspThrArgPheThrGlnProAlaLeuTyrValVal505560AsnAlaLeuAlaTyrTyrAlaArglieGluGluAlaAlaLysProPro65707580AspPheValMetGlyHisSerLeuGlyGluPheAsnAlaLeuLeuAla859095AlaGlyCysPheAspPheLeuThrGlyLeuGluLeuValArgPheArg[2015]100105110GlyGlnLeuMetGlyArgAlaGluAsnGlyAlaMetAlaAlalieVal115120125AsnAlaAsnGlnAlaThrlieGluArgThrLeulieGluAsnGlyLeu130135140ThrGlnLeuSerLeuAlaAsnTyrAsnThrProSerGlnlieVallie145150155160SerGlyAlaAlaAspGluMetAlaLysAlaGlnLeuLeuPheAsnSer165170175GlyAspMetArgCysTyrProLeuAsnThrSerGlyAlaPheHisSer180185190ProPheMetArgSerAlaArgGluGluPheAlaArgPheLeuGlnSer195200205PheArgPheAlaAlaProSerValProVallieSerAsnValSerAla210215220ArgProTyrGluSerAlaGlylieAspThrAlaLeuAlaSerGlnlie225230235240AspArgProValArgTrpCysAspSerlieGlnTyrLeuLeuGlyVal245250255AlaAlaArgArgGlyGluGlnlieGluPheGluGluLeuGlyAsnGly260265270AspValLeuThrArgLeuValGlnAlalieArgGlnGlnThrSerPro275280285GluVallieAlaHislieAlaThrGluValGluThrGlySerThrVal290295300AlaProMetGlyAlaValAlaAlaAspAlaArgSerAlaAlaAspLys305310315320ValAlaAlaTrpAsnArgArgTyrProValGlyThrArgValThrSer325330335LysHisProGluTyrArgAspLeuValThrArgThrGluAlaValVal340345350LeuPheAsnGlnArgAlaAlaValTyrMetGluGlyTyrHisGlyTyr355360365PheAspLeuAsnGluLeuThrAlaAla370375<210>10<211>341<212>PRT[2053]<213>Psuedomonassp.NO.2663[2054]<400>1MetThrAlaLeuLeuAspGlnLeuArgSerAlaAspAspPheGlnlie151015GluLeuLeuPheProGluGlnGluPhePheLeuArglieGlnAlaThr202530SerArgLeuProAlaValGluTrpValThrArgHisArgGlnMetLeu354045[2061]ArgGluAlaLeuLeuArgTyrGlyValValLeulieArgGlyLeuAsp505560CysAspArgLysAlaPheGlyThrlieAlaGluArgLeuGluProGly65707580GluPheAspTyrThrAlaGlySerGlyProHisThrSerValAspAla859095AsnValPheThrlieAspValProGlySerMetAlaLeuProGlnHis100105110AsnGluMetAlaTyrAsnLeuTyrTrpProMetHisValLeuPhePhe115120125CysGluGlnProProAlaProGlyThrGlyGlyThrThrSerValCys130135140AspAlaArgGlnPheLeuArgAspMetAsnProThrlieLeuAspPro145150155160PheSerLysPheGlylieGlnTyrValArgAsnPheProLysAsnMet165170175ProTyrLysSerlieGlyAspThrPheGlyThrSerAspArgAspLys180185190ValAsnAlalieCysAlaGluArgLyslieGluProlieTrplieAsn195200205AspAspHisLeuGlnlieArgGlnTyrAlaThrAlaValArgArgHis210215220ProValThrAlaGluGluSerPhePheSerSerValCysValCysHis225230235240ProAlaGlyTrpTrpAspLeuLeuArgArgAlaTyrProAlaAlaSer245250255LeuProArgSerGlnAspGlulieTrpGlnThrAlaLeuTyrGlyAsn260265270GlyAspProlieProAspAspVallieHisHisLeuLeuHisAlaTyr275280285GluHisArgGluTyrHisValMetTrpGluArgSerAsplieLeuTyr290295300[2093]lieAspAsnMetArgAlaSerHisGlyArgArgAlaCysThrGlyPro305310315320ArgAlalieLeuGlySerPheArgThrSerMetSerMetArgGlnLeu325330335GluAlaGlyHisSer340<210>11<211>482<212>PRT<213>Psuedomonassp.NO.2663<400>1MetSerAspAlaGlyAsnValGluAsnAspGlyLysSerlieLysGly151015lieAlaValAspProGluValLeuSerlielieLysAsnProAspArg202530ProGluAlaPheProSerlieAlaLysAlaProAsnAsnlieProGly354045ValSerGlyPhelieAlaGlyMetLysSerLeuPheAlaTyrLysArg505560GluGlyLeuGlnPhePheAlaAsnGlnAlaSerlieTyrGlyLysVal65707580PheArgSerGlnLeuGlyProTyrSerlieValGlyValThrAspHis859095GluVallieSerGlnlieLeuLysAsnGlyAspArgCysTrpSerThr100105110AlaLeuAlaValGluArgValThrArgGlyLeuSerPheAsplieAsp115120125LeuLysLysLysSerArgLeuMetSerLeuAspPheAspAlaHisArg130135140ArgMetArgAsnLeuValGlnProAlaPheSerAspArgAlaLeuAla145150155160SerTyrLeuThrVallieHisHisGluPheValHisAsnlieGluAla165170175TrpProArgAspGlyAlaValSerPheArgValGluAlaArgLyslie180185190PheSerGlnLeuAlaAlaArgLeuPhePheGlylieGlyAspProLys195200205GluAlaAlaArgLeuGluGlyAlaThrArgAspTyrTrpGlyAlalie210215220[2132]ProValValLeuLysAsnArgHisLeuSerGluArgTrpLysArgGly225230235240ValAspGlyValAlaThrLeuSerSerlieMetTyrAspLeuliePro245250255LysArgArgAspGluGlyGlyValAspLeuPheSerGlnLeuCysLys260265270SerArgAspAspThrGlyGlyLeuAspAspAspGlnLeuValAspLeu275280285MetLeuAsnlieliePheAlaAlaPheAspThrThrThrMetGlylie290295300ThrSerMetAlaTyrLeuLeuAlaLysTyrProHisTrpGlnGluVal305310315320LeuArgAlaGluAlaMetArgLeuProGluGlnAlaThrLeuAlaAsp325330335PheAsnGlyMetGluArgThrThrGlnValTrpLysGluThrLeuArg340345350MetPheProValSerAlalieThrProArgValSerLeuGlyGluCys355360365GlnValGlySerTyrValAlaProProArgThrLeuValPheAsnPhe370375380ThrGlyLeuLeuGlyGlnAspProAspLeuTrpAspHisProGlnArg385390395400PheAspProGluArgPheSerSerAlaArgAlaGluHisGlnArgHis405410415ArgGlyGlnTyrLeuProPheGlyHisGlyValHisThrCysValGly420425430AlaAlaLeuAlaAsnLeuGluAlaArgValPheTrpSerlieValLeu435440445ThrArgTyrArgPheSerLeuAlaLysAspTyrAspAlaValHisCys450455460MetSerProLeuGlySerValSerGlyGluValAlaLeuLysValGlu465470475480ArgLeu<210>12<211>152<212>PRT<213>Psuedomonassp.NO.2663<400>1MetThrlieAlalieGluSerProAspGlnProAspVallieAlaLeu[2171]151015lieAlaGluLeuAspAlaTyrGlnAspSerLeuTyrProProGluSer202530ArgHislieLeuAspLeuAlaAlaLeuLysGlnSerAsnValLeuSer354045AlaValAlaArgAspSerAlaGlyAsnAlalieGlyCysGlyAlalie505560ValLeuTyrProGluPheGlyGluLeuLysArgMetTyrValSerPro65707580ArgGlyArgGlyGlnGlyValAlaLysArgLeuLeuAlaLeuLeuGlu859095SerLysAlalieGlyLeuGlyCysLysLeuLeuLysLeuGluThrGly100105110ProTyrGlnHisGluAlaLeuAlaLeuTyrAlaSerAlaGlyTyrGlu115120125ArgArgGlyProPheGlyAspTyrThrAsnAspProLeuSerValPhe130135140MetGlnLysArglielieAlaAla145150<210>13<211>467<212>PRT<213>Psuedomonassp.NO.2663<400>1MetSerlieArgValAlaAlaAsnGluProSerSerThrlieGlnLeu151015ValThrLysGlyTyrAlaLysLeuLeuLeuThrGlyPheAlaLeulie202530ProLeuTyrLeulieAlaTyrLeuTyrPhePheGlnAspProSerLeu354045LysPheGluAsnHisAlaPheHisGluLeuAlalieAlaAlaAlaThr505560LeuGluGlyGlyPheValThrTyrValCysTrpArgCysTyrCysLeu65707580SerGlyGluProLeuLeuArgTrpLeuThrLeuGlyPheLeuGlyPhe859095SerLeulieTyrAlaLeuHisGlyValPheThrGlyMetAlaHisHis100105110AsnlieTrpLeuPheLeuLeuTyrGlyProAlaSerArgLeuValMet[2210]115120125SerlieLeuliePhelieGlyGlnLeuThrTyrSerHisAlaAlaAsp130135140SerAlaThrLysArgAlaAspArgArgValTrpAlaThrTrplieAla145150155160LeuPheValLeulieAspLeuSerValAlaGlylieAlaTyrSerPro165170175ValAlaGlyAlaProTrpMetArgValSerMetGluGlyGlyAlaLeu180185190LeuLeuSerThrValAsnValValAlaLeuValGlnArgArglieArg195200205SerProLeuMetThrlieTyrGlylieSerValThrSerPheAlaLeu210215220SerSerLeuAlaPhelieLeuGlyValProTrpAsnHisMetTrpTrp225230235240LeuAlaHisAlaliePheAlaAlaGlyPhePheLeuLeuSerPheGly245250255ValValGlnAlaPheHisThrThrArgSerPheSerLyslieTyrSer260265270GlnGlyGluLeuPheAlaArgLeuArgGluAlaMetGlyArgThrGlu275280285ArgAlaLeuGlnGluLeuGlnArgThrAsnGluLysLeuGluHisMet290295300AlaAlaThrAspProLeuThrGlyAlaAlaAsnArgArgGlnPhelie305310315320AspArgValGluAlaGlulieAlaArgAlaLysHisAsnGlyAlaPro325330335PheSerLeuLeuAlaLeuAspLeuAspHisPheLyslielieAsnAsp340345350SerTyrGlyHisGlnAlaGlyAspArgValLeuGlnArgPheValThr355360365LysCysArgAspAlalieArgProPheAspGlyValAlaArgValGly370375380GlyGluGluPheMetValLeuLeuProGlnAlaAlaLeuAspThrAla385390395400ArgSerlieGlyGluArglieArgAlaAlalieAlaGlyThrProPhe405410415GluAlaAsplieGlyGlnProlieGluValThrValSerlieGlyVal420425430[2249]SerGluPheGlyArgAspGlyGluThrlieAspAlalieLeuArgLys435440445AlaAspGluArgLeuTyrHisAlaLysHisGlnGlyArgAsnArgVal450455460ValAlaAla465<210>14[2256]<211>5061<212>PRT<213>Psuedomonassp.NO.2663<400>1LeuSerSerArgAlaHisArgSerGluValTyrGlyLeuProArgGln151015CysProGlyArgAlaTyrProGlnArgMetAlaProlieCysLeuPro202530SerHisSerLeuArgProAlaProLysArgCysLeuLeuTrpSerTrp354045SerTrpSerTrpSerArgAlaAspValProPheValHisAlaLeuArg505560HisArgAlaGlyLeuLeuAlaGlyCysSerAlaProAspCysProLeu65707580AlaSerProGlySerThrValHisProLeuLeuGluGlnArgProAsp859095AsnAlaAlaAlaGlyProSerPheAlaGlnLeuGlyArgValCysSer100105110ProValProHisPheSerArgCysAlaArgGlnArgValSerAsnHis115120125ValArgAlalieAlaMetArgAsnAlaAspProlieHisLeuAspVal130135140ArgPheThrGlnMetAspValGluAlaPheAlaArgLeuSerArgAsp145150155160CysAsnProLeuHislieAspProAspTyrAlaArgArgSerThrPhe165170175GlyGlyCyslieValHisGlyAlalieGlyValLeuAlaAlaLeuAsp180185190ArglieGlyLeuAlaGluArgValArgValThrSerLeuSerAlaSer195200205PheLysAsnProMetPheValAspArgThrTyrThrCysGlnValGlu210215220[2288]ValGlnGlyAspAsnCysArgThrValThrLeuArgAspGlyThrThr225230235240LeuLeuThrGlnlieGlyValThrTrpAlaProlieAspGlyGluGlu245250255ProAlaSerHisThrGluProAlaLeuAlaSerSerValGluAsnlie260265270ValAspHisAspAspGlyAlaLeuGluAlaGlylieGluLeuSerGly275280285GluTyrProProThrGlyLeuGlyThrCysTyrProArgAlaLeuArg290295300MetPheGlyGluThrValLeuAspThrlieAlaLeuCysSerPheLeu305310315320ThrGlyMetLysLeuProGlyArgArgAlaLeuPheSerAlaLeuThr325330335LeuThrPheGluHisArgGluSerAspGlyAlaLeuAsnTyrLeuHis340345350AlaThrGlnSerPheHisArgAspTyrGlyLeuLeuGluThrArgLeu355360365ValValGlyAspAlaHisThrThrlieAlaGlnGlyGluLeuArgSer370375380PheValArgLysThrlieValAspLeuProValGlnGlyTyrLeuThr385390395400AlaArgSerAspSerAspAlalieArgLeuAlaGlyLysValAlaPhe405410415ValThrGlyGlySerArgGlyLeuGlyAlaAlaLeuValArgCysLeu420425430AlaValLeuGlyCysHisValTyrLeuAsnTyrLeuGlySerAlaAla435440445HisAlaArgAlaValValAspAspLeuGluSerHisGlyLeuSerVal450455460ThrLeulieLysGlyAspValGlyAspArgAlaTrpLeuAspValVal465470475480ArgMetGluLeuLeuAlaArgHisGlyArgLeuAsplieLeulieCys485490495AsnAlaCysGluProProLeuArgMetGluAspAspAlaProSerPhe500505510AlaValArgArgGluHisTyrLeuSerArgAsnLeuGlnLeuValAsn515520525ValProLeuAlaValPheSerAspLeuLeuAlaAlaSerGlnGlyVal[2327]530535540CysValAlalieSerSerGluMetValAspSerValProAlaGlyTyr545550555560AlaHisTyrValAspLeuLysArgGlnThrGluAlaCysValArgHis565570575LeuAlaAspThrGlnProSerlieArgThrLeulieAlaArgProPro580585590AsnLeuLeuThrGluMetSerAsnThrProThrArglielieAsnAla595600605HisProProAsnGlulieAlaMetArglieValAsnAlaLeuProVal610615620GlySerAspArgHisHisLeuLeuAlaSerPheThrAspAspAlaThr625630635640ProArgAlaProGluThrLeuMetValThrAlaSerPheAlaLeuAsp645650655AlaMetLeuGlyGluPheAspGlyTrpLeuThrCysLeuProGlyGlu660665670GlnAlaAlaArgGlnThrGlyTyrAsnArgMetMetGlnAspLeuLeu675680685SerProAspSerValLeuAsnlieAlaGluGlyThrValAsnLeuLeu690695700TyrLeuArgValSerAspTrpLeuHisAspArgGluAlaCysTrpGln705710715720AspGlnGlyLeuArgLeuSerGluGluGlyAspPhelieArgArgVal725730735ValAspAspPheValAlaAlaLeuHisGlyPheAlaGluArgSerArg740745750AlaTrpGluLeuLeulieValCysProAspProArgLeuAspProAla755760765AspValAlaGlyAlaGluLeuLeuAlaThrValHisAlaArgLeuArg770775780SerLeuProValGluLeuProGlyLeuThrThrValPheAlaCysAsp785790795800HisHisAlaLeuHisArglieAspAlaThrArglieAspAspAlaSer805810815ArgAsnArglieAlaHislieProTyrValGlnAspTyrPheGlyPhe820825830LeuAlaLeuLeuAlalieArgMetLeuAsnAlaArgArgGlnLeuPro835840845[2366]AlaLysValValValLeuAspCysAspAsnThrLeuTrpHisGlyVal850855860CysGlyGluGluSerAspValValLysLeuLeulieAspAspGluMet865870875880ArgAlaPheGlnLysSerMetLysAlaLeuLeuAlaArgGlyPhelie885890895[2372]LeuCysLeuCysSerLysAsnArgGluGluAspValTrpLysValPhe900905910AspGlnHisProAspMetlieLeuThrArgGluAsplieThrAlaAla915920925LyslieAsnTrpLeuProLysSerAspAsnLeuArgMetLeuSerAsp930935940GluLeuGlyLeuGlyMetAsnSerPheValPhePheAspAspAsnPro945950955960LeuGluCysAlaGluValArgAlaGlyAlaProGluValLeuThrVal965970975CysThrThrGlnSerGlylieAlaProSerGluTrpAlaGluArgLeu980985990TrpValPheAspGlnLeuThrHisThrArgGluAspSerAlaArgAla99510001005ArgMetTyrArgGluGlnSerGluArgGluArgPheArgGlnSerThr101010151020GluSerPheArgValPhelieAspGlyLeuAsnLeuAsnValAlalie1025103010351040GluProLeuThrAlaGlyGlyValAspArgAlaSerGlnMetThrMet104510501055ArgThrAsnGlnPheAsnAsnThrThrlieArgArgAspLysAlaGlu106010651070lielieAlaMetMetAsnAspAspThrTrpArgLeuValThrlieAsp107510801085VallieAspArgPheGlyAspTyrGlyThrAlaGlyPheValAlaTyr109010951100ArglieGluLeuSerCysLeuTyrValAspAlaPheLeuLeuSerCys1105111011151120ArgValLeuGlyArgThrValGluAspArgValAlaArgTyrLeuAla112511301135ThrValAlaGlyGlnHisGlyValGluHislieHisValAlaPheVal114011451150SerThrArgLysAsnMetProPheGlnGlnPheLeuAspLysLeuAla[2405]115511601165ProSerGlulieMetValAspAspGluAlaArgGlyGluThrGlyArg117011751180TyrValPheGluLeuGlyAlaLeuArgGluThrLeuAspArgGlnAla1185119011951200TrpLeuGluHisGlyProArgAlaGluProAspGlyAlaSerSerAla120512101215ProProPheValValGlyAlaSerProProlieGlnGlyThrAspTyr122012251230ValGlylieAlaGluArgSerAsnArgAlaPheAspAlalieGlyAla123512401245SerGlyAspGluGluProAlaSerGlyAlaAlaLeuLeuProAspThr125012551260LeuAlaCysGlyGluThrGluLeuValAlaAlaAlaSerGlyAlaGlu1265127012751280HisGluAlaAlaLeuLeuArgGluValLysLeuCysTyrAlaArgVal128512901295LeuSerlieAspProAspThrLeuSerGluTyrGluGluLeuGluArg130013051310TyrGlyLeuGluSerlieAspValValAsnLeuThrValGluLeuThr131513201325ArgLeuValProArgLeuGlyProThrPheLeuPheAlaHisArgThr133013351340LeuArgAspValValArgGlyLeulieAlaGluTyrAspAlaArgAla1345135013551360ProGlyValMetProGluProLysLysAlaThrValGlySerAlaGlu136513701375SerAsnlieArgSerAspGluAlaAspGluHisHisAspAsplieAla138013851390lieValGlyLeuHisGlyValTyrProGlyAlaAspAspMetGluAla139514001405PheTrpGlnLeuLeuArgAlaGlyGluSerHislieValProMetPro141014151420LeuAlaArgArgHisLeulieAspArgAlaLeuProGlyValAlaSer1425143014351440ThrLeuHisGlyTyrMetAspArgAlaGlyTyrLeuAspSerlieGlu144514501455CysPheGluAlaGluLeuPheGlylieThrProLysGluAlaGluLeu146014651470.[2444]MetAspProGlnGlnArgLeuPheLeuGlnValValTrpGlyLeuLeu147514801485GluAspAlaGlyTyrThrArgGlnMetLeuAspArgAlaThrGlyVal[2447]149014951500PheValGlyValLeuSerSerAspTyrAlaMetTyrAlaAsnLeuHis1505151015151520AlaLeuGlnTyrProGluGlnTyrArgHisThrAspTyrTyrGlnlie152515301535AlaAsnArgValSerTyrHisPheAspLeuAsnGlyProSerMetSer154015451550lieAspAlaAlaCysAlaSerSerGlyThrAlaPheHisLeuAlaCys155515601565GlnAlalieArgAspGlyAspCysGluSerAlalieValGlyGlyVal157015751580AsnLeulieLeuHisProSerArgLeuValGlnTyrThrGlnThrGly1585159015951600MetLeuSerGlySerGlySerCysThrProPheGlyHisAspAlaAsp160516101615GlyThrLeuLeuGlyGluGlylieGlyAlaValLeuLeuLysArgLeu162016251630SerArgAlaArgValAspGlyAspAsnlieHisAlaValValLysAla163516401645SerSerValAsnSerGlyGlyLysThrAsnGlyPheThrValProAsn165016551660ProAsnAlaGlnAlaSerLeulieAlaGlyAlaLeuArgAlaSerArg1665167016751680ValProValGlnGlnlieAlaTyrValGluAlaHisGlyThrGlyThr168516901695AlaLeuGlyAspProlieGluValGlyAlaLeuGlyArgAlaPheArg170017051710GluLeuGlyAlaThrGlySerArgArglieGlySerValLysAlaAsn171517201725ValGlyHisGlyGluSerAsnAlaPheLeuAlaSerLeuSerLysVal173017351740LeuLeuGlnPheArgHisArgGlnTrpValProThrLeuThrSerAla1745175017551760GlnAspAsnThrAlalieAspPheSerAlaAlaGlyPheGluValGln176517701775ArgThrLeuAlaAspTrpProValLeuSerAspGlyGluSerValSer[2483]178017851790AlaCyslieSerAsnPheGlyAlaGlyGlySerAsnAlaHisValVal179518001805LeuGlnAlaTrpProAlaSerAlaGlyGluGlyArgAlaGlyArgHis181018151820LeulieProLeuSerAlaHisArgProAlaLeuLeuProArgLeuAla1825183018351840ArgArgLeuArgAspArgLeuMetArgAspAspProAlaThrlieAsp184518501855LeuAspAlaValAlaTyrThrLeuGlnValGlyArgHisHisLeuAsp186018651870HisArgValValPheValValAspSerArgAspAlaLeuLeuAspAla187518801885LeuGluArgPheGlnGlyGlyValThrSerAspAlaArgTrpMetCys189018951900GlySerThrThrValArgAsnArgLeuGlyGluLeuLeuAsnGluGln1905191019151920ValAspMetArgAlaLeuLeuTyrLysTrpGlyArgGluGlnAlaHis192519301935HisGlnlieAlaGlnLeuTrpLeuAsnGlyLeuSerlieProTrpAsp194019451950AlaLeuHisGluThrArgLysProValArgValSerLeuProThrTyr195519601965ProPheAspGlySerProHisTrpLeuGluHisThrHisAlaAlaVal197019751980LysPheGlyAlaGluSerSerAlaSerSerSerAlaSerAlaSerAla1985199019952000AlaAspProAlaLeuPheAlaProThrTrpThrArgAlaAspLeuPro200520102015ThrAlaSerValSerMetSerAspLeuValAlaHisHislieValPhe202020252030CysGlylieGluAlaAspAlaGlnAspAlaLeuArgThrAlaSerArg203520402045ThrHisAspThrArgValHisPheValProGlnThrAlaArgProSer205020552060GluThrAspTyrAlaGlyGlnAlaThrAlaLeuMetGlyTrpLeuArg2065207020752080GluTrpLeuArgGluGlyLeuArgGluArgLeuHisLeuGlnValThr208520902095[2522]ValValGlyAlaGlyAspGluAlaTrpThrGlyLeuAlaAlaLeuLeu210021052110LysSerAlaMetGlnGluAsnProLeuLeuSerAlaGlnLeuValAla211521202125PheAspGluAlaProAspAlaThrAlaTrpPheAlaArgLeuArgAsp213021352140GluMetArglieGlyHisAspValSerlieSerTyrArgProGluArg2145215021552160HisValArgThrTrpTyrProLeuAlaProProAlaArgProPhePhe216521702175GluGlyLeuAlaGlyValAlaAspGlyAlaGlyAlaTyrLeulieThr218021852190GlyGlySerGlyAlaLeuAlaLeuGlnCysAlaGluTrpLeuCysGlu219522002205ArgAspArgAspThrThrlieValLeuLeuAlaArgArgAspGluAla221022152220GluLeuMetArgSerAlaGlnGlyProArgPheAspAlaLeuArgAla2225223022352240AlaGlyArgArglieAlaTyrArgSerValAspValCysAspGlyGln224522502255AlaLeuAspAlaCysValAlaGluValValAlaThrHisGlyProlie226022652270ArgGlyValLeuHisCysAlaGlyVallieArgAspGlyPheLeulie227522802285HisLysSerGluAspAspPheGlnAlaValLeuAlaProLysValGly229022952300GlyValLeuAsnLeuAspArgAlaThrLeuAspGlnProLeuAlaPhe2305231023152320PheAlaLeuPheSerSerlieSerGlyValLeuGlyAsnAlaGlyGln232523302335AlaAspTyrAlaAlaAlaAsnAlaPheMetAspAlaPheAlaHisArg234023452350ArgAlaAlaArgGlnArgAlaGlyGluArgThrGlyArgSerValSer235523602365lieAsnTrpProLeuTrpArgGlyGlyGlyMetMetLeuAspAlaGlu237023752380LeuAlaThrLeuLeuArgGluGlnHisArgLeuAspAlalieThrGly2385239023952400GluAlaGlyMetArgAlaLeuAspArgAlaLeuAlaSerAsplieGln[2561]240524102415GlnValPheValGlyThrGlyAlaAlaAspAlaValAlaHisThrLeu[2563]242024252430AlaAlaSerAspArgArgGlnProAspAspAlaAlaGlyThrAlaAla243524402445ProLeuGluAlaGluSerAlaThrSerAlaProAsnAlaAlaAspLeu245024552460TyrProTyrLeuLysArgMetLeuAlaAspAlaThrLysLeuAspPro2465247024752480ValThrlieAspGluHisArgProLeuAlaAspLeuGlyLeuAspSer248524902495lieLeuLeuAlaLysLeuAsnLysValLeuAlaValAsnPheArgGln250025052510LeuProArgThrLeuPheTyrGluTyrProThrLeuAlaAlaLeuAla251525202525AlaTyrLeuAlaGluGlnlieArgTyrGlnGlylieProAlaGluArg253025352540PheAlaAlaAlaArgAlaThrValGluGlnProAlaProAlalieAla2545255025552560ThrSerProAlaGlyGlyGluProThrArgArgProLeuLysSerGly256525702575AlaSerGlyAlaArgGlyValAspAspProlieAlalielieGlylie258025852590AspGlyArgTyrProAsnAlaProSerPheAspAlaPheTrpGlnArg259526002605LeuLysThrGluSerSerGlulieGlyGluLeuProValHisArgTrp261026152620HisGlyProGlyAlaAspAlaGluArgAspLeuHisProThrLeuArg2625263026352640AsnArgLysGlyAlaPheLeuAspAspPheAlaGlnPheAspProThr264526502655PhePheGlnlieAlaProlieAspAlaTyrSerlieAspProGlnGlu266026652670ArgLeuValLeuMetSerCysTrpThrAlaLeuGluSerSerGlyTyr267526802685ThrArgGluArglieAlaSerGlnHisGlyArgAsnValAlaValCys269026952700ValGlyAlaThrLysSerGlyPheAsnGlnSerSerSerGlyThrVal2705271027152720[2600]ProArgAlaAspAspMetAspAlaGlyAlaArgGluProValThrSer272527302735ThrSerPheAlaAsnMetAlaAsnArgValSerTyrPheLeuAspLeu274027452750HisGlyProSerlieAlaPheAspThrMetCysThrSerSerLeuThr275527602765AlalieHisHisAlaCysGlnTyrLeuArgGlnGlyGluValGluLeu277027752780AlalieAlaAlaGlyValAsnLeuTyrLeuHisProAsnAspTyrLeu2785279027952800AlaLeuAspArgLeuGlyMetLeuSerAspGluAlaArgValAsnCys280528102815PheSerLeuGlyGlyAsnGlyPhelieProGlyGluAlaValGlyAla282028252830ValValLeuLysArgLeuAspArgAlaLeuAlaAspGlyAspArglie283528402845AspGlyVallieThrAlaThrGlylieSerHisSerGlyArgThrHis285028552860GlyTyrThrAlaProSerValThrGlnGlnGluThrLeulieGlyArg2865287028752880VallieGluArgAlaGlyArgHislieGluGlulieAlaTyrlieGlu288528902895SerAlaAlaAsnGlySerSerLeuGlyAspAlaValGluTrpGlyAla290029052910LeuLysLysValPheHisSerArgThrGlnProCysProlieGlySer291529202925ValLysProAsnlieGlyHisProGluAlaAlaAlaGlylieValGln293029352940LeuSerLysVallieAlaGlnLeuArgHisArgAlaLeuAlaProThr2945295029552960LeuAlaAspSerSerArgLeuAspHisAspLeuLeuAlaAspSerGly296529702975LeuArgLeuValThrArgTyrGluProTrpProGluSerGlyProSer298029852990AlaCysLeulieThrSerAlaGlyAlaGlyGlyThrTyrAlaAlaMet299530003005ValValGluProAlaProValThrGlyAspAlaProValGluArgAla301030153020AspAlaValAlalieAlaLeuSerAlaArgSerSerGluAlaLeuArg[2639]3025303030353040ArgValAlaAlaGluLeuHisAspGlnLeuAlaAlaTyrProAspLeu304530503055ArglieGluAspValAlaArgThrLeuHisMetGlyArgGluAlaMet306030653070LysValArgThrGlyCysValValGluSerArgArgAlaLeuMetSer307530803085ArgLeuArgAlaMetThrSerGlyGluProAlaGlyGluAlaAspAla309030953100SerArgAspThrValAlaThrValProLeuArgValArgLeuGluAla3105311031153120AlaLeuArgValTrpLeuAlaGlyHisAspValAspTrpProAlaAla312531303135TyrGlyAspAlaTrpAlaSerAlaHisValAlaProArgAlaLeuPro314031453150GlyTyrProPheAspThrArgGluTyrTrpArgGluProSerHisAla315531603165ArgArgAlaGlyGlyLeuValProAlaArgArgValLeuProAlaLeu317031753180ProProAlaValAlaThrAlaSerValProLeuSerAlaValProSer3185319031953200ArgAlaGlnlieGlnAlaAlaProSerAlalieLeuAlaGluValLys320532103215AsnAlalieArgAlaLeuLeuHisValGluProAlaGluGlulieAsp322032253230AspGluAlaThrPhePheGluLeuGlyValAspSerlieArgMetVal323532403245SerPheValArgGlyMetAlaSerArgLeuSerlieAlaLeuAspGlu325032553260ThrAlaLeuPheAspValProThrValAlaArgPheSerAlaHisLeu3265327032753280AlaArgLeuArgGlyAlaArgAspAlaProAspAlaLeuAspThrAla328532903295AlaSerProLeuValProThrAlaArgAspAspArgThrArgValAla330033053310lieGlyGlyProAspGluGlyVallieLeuArgGlnLeuLysThrVal331533203325ValAlaVallieLeuAspTyrProAspProAlaValLeuAspAspAsp333033353340[2678]ValThrPhePheGluLeuGlyValAspSerlieArgMetValGlyPhe3345335033553360[2680]ValGluAspLeuSerAlaArgLeuAlaLeuAspLeuGluGluThrVal336533703375LeuPheAspTyrProThrLeuAlaAlaMetAlaArgHisValAlaThr338033853390ArgMetGlnValArgAsnThrAlaAlaThrAlaAlaProAlaSerArg339534003405GluValProAlaGluThrArgArgGluAlaAlaGlyGlyProAspAla341034153420AlaProLeuGlyHisGluAlaLeuLeuAsnAsnLeuArgArgHisGly3425343034353440ArgThrTyrGluGlyValValProLeuGlnThrGluGlyAspGlyPro344534503455LeuValPheCysLeuHisProAlaSerGlyAspValAlaMetPheGly346034653470LysLeuAlaMetAlaAlaGlyAspArgPheArgVallieGlyLeuLys347534803485AlaArgGlyPheLeuThrAsnGlnProHisCysAlaAspAlaGluGlu349034953500MetAlaArgTyrHisAlaGlulielieGlnAlalieAspProValGly3505351035153520ProTyrHisValMetGlyAlaSerMetGlyGlyValValAlaTyrGlu352535303535ValAlaArgAlaLeuGlnAspAlaGlyArgGlnValArgThrValPhe354035453550MetLeuGluAlaProLeuValThrCysGluArgAspSerAspliePhe355535603565ThrLeuAspAlaLeuGlnAsnTrpLeuAlaAsnAlaAsnPheLeuMet357035753580lieAlaMetLeuHisLeuAspProAlaPheArgGlnArgLysGluAla3585359035953600GlyLeuValLysTrpAspAspLeuVallieThrAlalieGluLeuGly360536103615LeuAspGluSerAlaLeuArgAspAspSerGlnValGluSerArgLeu362036253630ValAspLeulieValGlyArgGlyValArgGlnThrProGlylieLeu363536403645ArgSerArgLeuArgSerMetAlaAspThrHisArgAsnAsnLeuArg[2717]365036553660CysMetArgGluTyrArgTrpArgProLeuValArgProAspGluVal3665367036753680GlulieLeuMetPheArgThrMetThrAlaArgAlaThrSerAspGln368536903695ValTyrAsnProAsnTyrLeuGluAsnLeuGlnArgValHisGlySer370037053710MetMetProMetLeuAspAlaTrpValGluArgMetSerGlnValArg371537203725ThrValValValGlyGlyGluAspHisPheAspLeuCysAsnArgThr373037353740LeuGlyAspGluAlaVallieGlyProMetLeuAlaSerMetArgArg3745375037553760AlaGluProGluProGluProGluAspGlnGlyAspThrArgSerSer376537703775AlaPheArgValAlaVallieGlylieSerAlaArgPheProGlyAla378037853790AspHisValAspAlaPheTrpAsnValLeuAlaSerGlyGluSerLeu379538003805lieArgAlaValProAlaGluArgPheGlyLeuGlnAspAspAlaPro381038153820AspHisGlyCysLeuPheGluArglieAspGluPheAspHisAlaPhe3825383038353840PheAsnLeuSerProAsnGluAlaArgMetMetGluProAlaGluArg384538503855MetPheLeuGlnGluAlaTrpHisAlaValGluAspAlaGlyLeuVal386038653870ProAspArgGlyAlaGlyGluAsnTrpGlyValPheAlaGlySerGly387538803885GlyAspTyrAsnLeuHisValGlnSerValLeuGlylieAlaProGln389038953900ValSerThrSerThrValProGlyArgValAlaTyrGlnLeuAsnLeu3905391039153920HisGlyProThrMetThrlieAspAlaGlyCysAlaSerSerLeuLeu392539303935AlaValGlyGlnAlaCysAspSerLeuArgLeuGlyGlnCysGluVal394039453950AlalieAlaGlyGlyAlaMetValTyrSerThrArgAsnLeulieAla395539603965[2756]ThrGlyHisTyrTyrArgLeuLeuSerLysThrAlaArgGlyAlaAla397039753980LeuAspAlaGlyAlaThrGlyMetLeuProGlyGluAlaValGlyAla3985399039954000ValValLeuLysProLeuAspAlaAlaLeuArgAspGlyAspArglie400540104015TyrAlaValLeuAspAlaTrpGlySerAsnHisAsnGlyArgThrGln402040254030GlylieAlaAlaProSerAlaAlaSerGlnAlaAlaLeuLeuThrSer403540404045lieHisArgArgPheGlylieAspProAsnThrValAspPhePheGlu405040554060AlaAsnAlaSerGlyAlaProLeuGlyAspLysValGluLeuGlnGly4065407040754080LeuArgMetValPheGlyGlyArgProArgAlaHisProLeuAlaLeu408540904095GlyThrValGluAsnThrValGlyHisSerPheAlaAlaSerGlyMet410041054110AlaHisLeuLeuLysVallieLeuAlaLeuHisArgGluMetLeuPro411541204125ProThrAlaAsnValAspAlaAlaThrAlaValAlaGlyGlyAspAsp413041354140GlyLeuPheGlulieProAlaArgAlaArgProTrpProSerGlyThr4145415041554160GlyAlaAlaArgArgAlaGlyValSerSerTyrSerAlaThrGlylie416541704175AsnValHisLeuValValSerGluAlaProProAlaAlaAlaAlaThr418041854190SerValThrGlyProThrLeuLeuAlaPheSerAlaAlaThrProGlu419542004205AlaLeuArgGlnArgGlyArgAspLeuLeuAspTyrValAlaAlaMet421042154220AspAspGlnSerTrpAlaProAlaArgLeuSerAlaHisLeuLeuSer4225423042354240ArgArgLeuHisPheArgHisArgCysAlaLeuLeuAlaArgAspArg424542504255AlaAspAlaGlyArgLeuLeuAlaArgPheValAlaAsnGluAlaAsp426042654270AlaGluValLeuThrAsnThrValGlyAlaThrThrAspAspAspPro[2795]427542804285ThrArgGluAlaLeuArgArgGluAlaAlaAlaPheGlyAlaThrArg429042954300[2798]AlaAlaLeuProLeuAspArgAlaAlaGlnTrpTyrValHisAsplie4305431043154320AlaPheAspAlaArgGlyGlyPheAlaSerValAspArgValProLeu432543304335SerLeuProGlyTyrProPheAlaArgSerArgHisTrpLeuGluAla434043454350SerProValAlaGlnAspValAlaAlaGlnProValThrGluAspPro435543604365ArgHisTrpLeuLeuArgGlnlieAlaVallieThrGlyGluValPro437043754380AspAlaAlaGlnAlaGluAlaSerTrpSerAlaMetGlyPheAspSer4385439043954400LeuMetSerlieArgLeuLeuSerThrlieGlnGluGlnThrGlylie440544104415GlulieProLeuValAspLeuLeuAspGlnSerThrProAlaAlaLeu442044254430LeuAlaHisLeuAspAlaLeuGlyAlaPheAlaHisGlyGlnCysGly443544404445GluAlaProGlyProAspThrSerProLeuProProValGlnTyrAla445044554460TrpPheGlyAspArgLeuAlaAlaLeuArgAlaThrAlaGluValAla4465447044754480ThrValAlaLeuArgProGlnAlaProThrProAlaSerArgSerHis448544904495ArgAspArgMetLeuAlaGluLeuLeuAspAlaGlylieAlaValPhe450045054510ArgAspThrAspLysAlaValLeuLeuAlaAlaArgSerValGlylie451545204525AspAlaArgLeuArgArgLeuAspGlnAspThrLeuAlaAlaCysLeu453045354540GlyMetLeuGluProGlyGlnLeuTrpAlaProLeuSerGlnGluGln4545455045554560AlaArgAspLeuHisTrpThrGluArgMetGlySerThrAlaTrpAsn456545704575ValPheHisValPheArgLeuTyrAlaHisGluValAspLeuGluArg458045854590[2834]LeuArgHisAlaLeuThrLeuLeuAlaGlnGluGlnAspLeuLeuArg459546004605ThrArgTyrlieThrValGlyAspGlyHisAlaGlnCysValSerGlu461046154620LeuValCysValSerLeuAspSerlieSerlieAspSerSerGluAla4625463046354640PheHisGlnPhelieGlnSerArgArgSerHisProLeuLeuGluGlu464546504655HisGlyGlyProAlalieArgMetTrpLeuThrGluValAspGlyAla466046654670PheHisLeuGlyMetValAlaHisHisAlaHisAlaAspAlaPheThr467546804685ProGlyLeuLeuPheSerGlnLeuMetAlaLeuTyrGlySerLeuGly469046954700SerGluAlaGlyAlaArgAspAspThrArgArgTrpAlaAspGlnTyr4705471047154720TrpPheTyrAlaLeuArgGlnHisAspAlaArgGlyAlaSerAlaAsp472547304735AlaHisArgAlaTrpTrpArgAspThrLeuAlaGlyValGluValGlu474047454750MetArgLeuProTyrArgLysGluValThrSerSerProSerLeuArg475547604765AlaLeuAlaThrAsnGlnlieValProValSerAlaGluLeuGlyGlu477047754780ArglieLysAspLeuSerArgValHisGlylieSerTyrProGlnLeu4785479047954800PheAlaAlaValLeuAlalieValLeuArgGluCysPheGlyAsnAsp480548104815ArgProValLeuGlnVallieHisAsnGlnArgAspSerAlaAlaLeu482048254830AlaAlaCysLeuGlyAspPheSerAsnValAlaPheValProPheGlu483548404845LeuAspProGlyArgSerVallieAspThrLeuTyrAlaValArgArg485048554860ValLeuLeuThrAlaLeuGlnHisThrArgValProMetAlaAspLeu4865487048754880PheArglieAlaGlyValAspGlyHisThrGlyTyrGlyAlaMetVal488548904895GlyAspValValLeuAspSerAlaAspMetAspThrAlaAsnValAsn[2873]490049054910lieAlaGlyGluHisGlyValSerThrTyrMetAsnAlaValLeuGlu491549204925[2876]GlnArgSerlieHisPheLeuAspGlnAlaLeuAlaSerLeuPhePhe493049354940GlnPheLeuArglieAspGlyArgLeuTyrliePheHisAlaTyrArg4945495049554960ArgAlaLeuPheGluGlnGluThrMetGlnLysLeuProGlnLeulie496549704975ValGluLeuAlaAspAlaMetLeuArgThrProAspValProValAla498049854990AspLeulieAlaAlaPheGlyArgArgPheAspGluLeuGlyArgGly499550005005GlyArgAlaProThrMetArgAspAlaProAlaAlaAlaValThrGly501050155020AspSerAlaAlaLeuLeuGluArgPheLeuGlyGlyAspLeuSerlie5025503050355040AlaGlnLeuArgGlySerLeuHisProAlaGlyThrArgAspArgGly504550505055ArgValValAlaGlu5060<210>15<211>1770<212>PRT<213>Psuedomonassp.NO.2663<400>1MetArgArgGlyHisAspGlySerGlySerMetSerAspAlaLysArg151015LeuAlaAlaProGlyAsnValGluAspGlyLysArgGlulieValPro202530ThrGluGluValAspAlaTrpLeuLysArgHisPheMetAlaAspAla354045GlnAlaProLeuSerArgAsnGlnArgGlylieTrpSerArgGlnMet505560LeuTyrProMetAspProLeuTyrAsnValAlaValAlaCysThrVal65707580GlyAlaGlyPheAspArgGlyLeuPheArgArgValCysGlnArgPhe859095LeuAsnHisHisGlnLeuLeuAsnAlaArglieValAlaGluGlyAsp[2912]100105110AlaLeulieGlnArglieAspSerMetArgGluLeuAlaTrpTyrGlu115120125[2915]SerAlaThrGlyGluSerLeuAspAlaCysArgAlaHisAlaLysArg130135140AspAlaAlaValProPheValLeuHisAspGlyProLeuTyrArglie145150155160HislieTyrGluAlaSerSerCysGluThrValValLeuLeuValVal165170175HisHislielieCysAspGlySerSerValSerArgTrpLeuSerThr180185190lieTrpGlnAspTyrArgAlaLeuLysGlnGlnArgThrLeuProGln195200205AlaLeuGlyGlyAlaSerPheLeuAspPheValGlnArgGluAlaAla210215220HisLeuAspSerAlaAlaGlyGlnLysSerLeuAlaTyrTrpArgThr225230235240GlnCysAlaAspGlyLeuProProLeuAspAlaAlaLeuAsnLeuPro245250255ProAlaAlaAspAspAlaSerValPheAlaThrSerArgLeuSerPhe260265270SerlieAspGlyAspArgAlaMetArgMetArgThrLeuArgLysThr275280285GlySerPheSerProSerThrLeuLeuMetGlyAlaPheThrLeuAla290295300LeuSerAlaTrpArgGlyThrArgArgPheThrLeuGlyLeuProThr305310315320LeuGlyArgProProGlyAlaGluPheAlaSerThrlieGlyAsnPhe325330335ThrAsnLeuValProValValCysAspLeuAsnProAlaGlnThrPhe340345350SerGlnTrpLeuAspGlyCysGlnArgTyrValTyrArgAlaLeuAsp355360365HisGlyTyrProPheAlaGlyLeuLeuGlnAlaLeuProArgHisAla370375380AspAlaProLeuSerlieGlnThrValPheThrTyrGlnLysPheGly385390395400AspGlulieAsplieGlyThrLeuProAspArgValGlyAspSerVal405410415[2951]GluLeuAlaPheGlyAlaAspliePheGlnArgAspGluTyrProVal420425430ThrCysGluValValGluAlaGlyAspGlyTrpThrValAsnLeuLys435440445TyrLysThrAlaVallieGluHisAspAspAlaGluAlaLeuArgHis450455460AlaPheValAlaValLeuAspAspAlaLeuThrAspProAspArgThr465470475480[2959]lieGlyAlaLeuThrAlaArgAspAspArgArgHisGlyAlaGlyPro485490495MetGlnProAlaSerHisGlyProAlaAlaAspAlaLysSerLeuCys500505510AlaArgPheThrAlaLeuSerArgArgAspProAlaArgArgAlaLeu515520525SerPheAspGlyArgHisMetThrTyrGlyAspLeuAspArgGlySer530535540AspAlaLeuAlaArgHisLeuValSerLeuGlyValArgProGlyAsp545550555560LeuValLeuLeuCysValThrProSerProAspLeulieThrAlalie565570575LeuAlalieLeuLysAlaGlyAlaAlaTyrValProlieAspProGly580585590SerProAlaGlnArglieGluMetlieArgGluAspSerGlyAlaArg595600605PheValValThrThrSerlieSerGluAlaAlaValGluSerValAla610615620HisArgValValLeuAspArgAspGlyAlaAlalieAlaAlaAlaVal625630635640GluArgGluProThrLeuValLeuProAspValArgArgGlyAspLeu645650655AlaTyrVallieTyrThrSerGlyThrThrGlyLysProLysGlyVal660665670MetlieGluHisGlyAsnValLeuArgLeuPheAspArgThrAsnHis675680685TrpPheAspPheGlyProAspAspValTrpThrLeuPheHisSerAla690695700AlaPheAspPheSerValTrpGlulieTrpGlyAlaLeuLeuTyrGly705710715720GlyArgLeuVallieLeuAspThrThrGlnArgLysAspProValArg[2990]725730735LeuLeuGluThrLeuAlaAspGluGlnValThrValPheAsnGlnThr740745750ProAlaAlaPheTyrAlaValValArgAlaAlaThrAlaGlyProPro755760765ArgArgProLeuAlaLeuArgLeuValValPheGlyGlyGluArgLeu770775780AspPheGlyArgLeuAlaProTrplieGluArgTyrGlyAspSerArg785790795800ProAlaLeulieAsnMetTyrGlylieThrGluThrThrValHisVal805810815ThrHisAlaProValThrAlaAlaAspValThrGlyAlaCysAlaSer820825830VallieGlyHisProlieProAspLeuAspValGlnLeuLeuAspGlu835840845HisArgAlaProValProAspGlyGlulieGlyGluMetTyrValGly850855860GlyAlaGlyValAlaArgGlyTyrLeuAsnArgProGluLeuThrGln865870875880GluArgPheValThrlieSerGlyValArgCysTyrLysThrGlyAsp885890895LeuAlaArgArgAsnAlaArgGlyGluLeuGluTyrlieGlyArgAsn900905910AspAlaGlnValLyslieArgGlyTyrArglieGluLeuGlyGlulie915920925GluAlaCysMetAsnGlnHisProValValGluGlnGlyValVallie930935940AlaHisGluHisAlaAsnGlyThrLysHisLeuValAlaTyrTyrArg945950955960AlaArgAspThrAlaThrAspAspGluAlaArgSerHisAspLeuArg965970975AlaPheValAlaAlaArgLeuProGluTyrMetValProAlaPhePhe980985990ValAlaLeuAlaGluLeuProLeuThrGlyAsnGlyLysPheAspArg99510001005GlnSerLeuSerArgArgProlieValLeuAlaSerThrAspSerAsp101010151020ValAlaGlyAlaSerAlaThrGlnGlnArglieArgGluLeuTrpGln1025103010351040[3029]GlnValLeuGlyValAlaProAlaHisPheAspGlnGlyPhePheGlu104510501055AlaGlyGlyAspSerLeuSerValValLeuLeuAlaArgAlalieGlu106010651070GlnAlaPheGlyHisAlaPheThrProAlaLysLeuPheArgTyrAla107510801085ThrValArgAsplieAlaAlaTyrLeuGluThrHisAlaThrArgPro109010951100AspSerSerAspAlaAlaValSerValGlyProArgAlaThrAlaAla1105111011151120AspGluLeuSerArgAlaSerProValValProAspTyrTyrAspGlu112511301135SerLeuAlalielieGlylieSerCysGluPheProGlyAlaAlaAsp114011451150HisLeuGluPheTrpAlaAsnLeuLeuAlaGlyArgGluSerValGlu115511601165TrpLeuThrArgAlaAlaLeuAspGluAlaGlyValAlaProAspLeu117011751180AlaSerHisProAspTyrValSerAlaArgPheThrlieProGlyLys1185119011951200ThrArgPheAspAlaAspPhePheLysLeuSerAlaLysLeuAlaGlu120512101215PheAlaAspProGlnSerArgGlnLeuLeuMetHisAlaTrpLysAla122012251230ValGluAspAlaGlyTyrValProGlyGlnPheAspAlaThrGlyVal123512401245PheValSerThrGlyAsnSerGlyTyrGlnThrLeuLeuAspAsnAla125012551260ProAsplieGluGlnGlnAspArgTyrLeuAlaTyrValMetSerGln1265127012751280GlyGlySerThrAlaAlaLeulieSerTyrGlnLeuGlyLeuThrGly128512901295ProSerMetGlnlieGlyThrAsnCysSerSerSerLeuValAlalie130013051310AspAlaAlaPheLysSerLeuArgAlaGlyGluCysArgGlnAlalie131513201325ValGlyAlaSerThrLeuPheProHisAlaAsnAlaGlyPheLeuTyr133013351340GlnProGlyLeuAsnPheSerSerThrGlyHisCysArgThrPheAsp[3068]1345135013551360ValAspAlaAspGlyMetMetAlaGlyGluGlyValAlaVallieVal136513701375LeuLysAsnAlaArgGlnAlaValGluAspGlyAspAlalieTyrAla138013851390ValLeuArgSerlieAlaValAsnAsnAspGlyArgGlnLysSerGly[3074]139514001405PheTyrAlaProSerAlaGlnGlyGlnGlyArgVallieArgThrAla141014151420LeuGluArgAlaAlaAsplieAspValGluSerlieAlaTyrValGlu1425143014351440AlaHisGlyThrGlyThrArgLeuGlyAspProValGluValMetAla144514501455lieAspGluValTyrArgGlnArgThrGlyAlaArgGlnPheCysAla146014651470lieGlySerValLysProAsnlieGlyHisLeuAspThrAlaAlaGly147514801485LeuAlaGlyCyslieLysValAlaLeuAlaLeuArgHisArgMetlie149014951500ProProThrlieAsnValThrThrProAsnProAlalieAspTrpAla1505151015151520SerSerCysPheArgValProHisGluAlaSerProTrpProGluGly152515301535AlaCysProProArgAlaAlaLeuSerSerPheGlyLeuGlyGlyThr154015451550AsnAlaHisAlalieLeuGluAlaCysProAspValArgAlaAlaSer155515601565ProAspArgAspAlaProThrGlyGluProArgLeuValLeuLeuSer157015751580AlaAlaGluProArgLeuLeuProThrMetAlaArgThrLeuSerVal1585159015951600PheLeuValValHisProAspLeuProPheAspAlaValAlaAlaThr160516101615LeuGlnArgGlyArgGlnProMetArgHisArgLeuAlalielieAla162016251630ThrGlyAlaGlnGluAlaAlaGluLeuLeuGluArgTyrAlaAlaGlu163516401645AlalieGluGlyAlaThrAlaAlaProTrpProAlaAlalieValCys165016551660[3107]SerArgProAspAlaAlaProSerAlaProGlyAlaAlalieCysArg1665167016751680ArgTrpProArgCysGlySerArgGlyThrMetSerThrGlyArgSer168516901695CysMetProAlaArgArgCysAlaAlaCyslieCysArgProlieArg170017051710SerHisAlaGlyAlaMetArgSerArgProGlyProArgProProGly171517201725ArgArgGlyArgAlaSerProPheSerSerArgArgThrAlaArgGly173017351740ArgSerThrSerGlyCysAlaAlaThrThrThrThrSerThrThrlie1745175017551760AlaSerThrAlaAlaAlaCysCysProPro17651770<210>16<211>1188<212>PRT<213>Psuedomonassp.NO.2663<400>1MetThrPheGluLeuLeuArgAlaAlaAlaMetThrAspAlaAlaVal151015ProAspGluAlaArgLeuGlnlieGluHisValValTrpLeuArgPro202530VallieCysAspGlyGluAlaLeuSerLeuCysValGluLeuAlaAla354045ArgGlyValGlyGlnAspTrpArgAlaProValAlaPheArgLeuThr505560GlyValAspAspAlaThrAsnGluArgValMetPheAlaGluGlyAla65707580LeuAlaPheGluAlaSerGlyLysValAspGlyThrAspGlyTrplie859095AlaProGluAlaLeuArgGlnAlaLeuThrGluAsnArglieAspGly100105110AlaAspTrpTyrArgGlylieAlaAlaGlyArgValGlnTyrGlyPro115120125ThrTyrLeuGlyLeuValGluAlaGlyTrpAsnAspMetGlyLeuThr130135140AlaArgValArgLeuProGluSerValSerSerSerLeuAlaThrPhe145150155160[3146]ProLeuHisProAlaLeuLeuAspCysAlaLeuGlnAlaSerValLeu165170175LeuAspArgValArgArgHisGlyGlyAspProGlyGlnTrpSerGly180185190MetMetProAlaArgGlnLysLeuProPhePheValGluArgValAsp195200205ValLeuAlaProLeuGluAlaGluValTrpValArgValGlnGlyHis210215220GluProValAlaThrAlaArgGluAlaArgTyrAspliePheValHis225230235240GlyAlaSerGlyAlaLeuLeuValAlalieArgGluPheValThrArg245250255AlaLeuProGlyGlyAsnGluAspArgAlaLeuProAlaProAsnPro260265270lieGluMetAlaAlaGlnGlnLysAsnAspAlaAspThrAlaProLeu275280285HisThrLeuThrlieAlaTrpThrProLeuAspGluArgArgGluAla290295300GlyValSerThrAlaProlieValLeuPheGlylieGlyGluValPro305310315320ProAlaGluLeuValAlaCysPheAlaGlyArgValValArgTyrVal325330335ProLeuArgSerGluProAspGlyProAlaLeuAlaAlaLeuProAla340345350AspAlaAlaLeuValPheAlaLeuProAlaGlyAlaAlaArglieAsp355360365AspAlaAlaLeulieAspAlaGlnArgAspGlyValValAlaValPhe370375380ArgTrpLeuArgAlaLeulieGlyHisGlyPheGluGlnValAspLeu385390395400AspCysThrPheValValGluArgThrGlnArgValArgAlaGlyAsp405410415SerlieAlaProValHisAlaGlyLeulieGlyLeuAlaGlnSerLeu420425430AlaGlyGluTyrGlyHisTrpArgValArgLeuLeuAspValGlyGly435440445PheAlaAspLeuProThrProAlaGlyTrpProAsnAlaGluGlyLys450455460ValValPheAlaTrpArgAspGlyArgTrpPheThrArgSerLeuVal[3185]465470475480GluThrAlaMetProThrSerCysProThrLeuTyrArgGlnGlyGly485490495HisTyrValValLeuGlyGlyAlaGlyGlylieGlyArgThrTrpThr500505510GluThrMetlieArgAspTyrArgAlaArgValValTrpLeuGlyArg515520525LysProArgAsnArgGlulieAspAspGlulieAlaArgLeuGlyLys530535540LeuGlyProAlaProlieTyrLeuGlnAlaAspAlaThrAspProAla545550555560SerLeuArgArgAlaArgHisArgLeuThrGluLeuGlyLeuAspThr565570575LeuHisGlyValValHisSerAlaLeuValLeuAspAspArgGlylie580585590AlaAlaMetSerAspAlaGlnPheAlaArgAlaLeuAspAlaLysVal595600605AlaThrSerValAsnLeuAlaArgValPheGlyAspGlyAspLeuAsp610615620PheMetLeuPheTyrSerGlyThrlieSerPheValAsnProAlaGly625630635640GlnSerAsnTyrAlaAlaGlyCysSerPheGlnAspAlaTyrAlaMet645650655AlaLeuAlaAspGlnArgArgCysAlaValLysLeuValHisTrpGly660665670TyrTrpGlyHisValGlyAlaValAlaThrProGluTyrArgArgArg675680685MetAlaAlaAlaGlyValGlySerValGluProGluAlaAlaMetGln690695700ArgLeuAlaGlnPheMetGlnAspAsnHisArgArgGlnlieAlalie705710715720GluSerArgLeuArgAlaArgGlyAlaAlalieAspThrProGlnAla725730735ThrAspAlaArgProAlaGlyThrProThrAspAlaHisArgSerHis740745750ProAspAlaGlnGlyTrpSerArgLeuLeuGlnArgValAspAlaLeu755760765ArgGluHisAsnGlyLeuArgAspProValValGluHisAlaAlaLeu770775780[3224]LeuMetValArgGlnValLeuGlyAlaLeuAspAlaLeuProValAsp785790795800ArgThrHisAspGlyAlaTyrArgAlaGlyProArgSerArgLeuAla805810815AlalieTyrGluArgTrpTyrGlnGluSerCysArgGlnLeuGlnLeu820825830HisAlaLeulieAspValSerGlyGlyAlaThrGlyHisAlaThrArg835840845AlaThrGluAspAlaHisAlaAlaTrpAlaArgValArgAspHisTyr850855860ArgAlaHisLeuSerValArgAlaGlylieGluLeuMetAspAlaCys865870875880LeuArgAsnLeuProAlalieLeuArgGlyGluLeuAlaAlaThrThr885890895ValMetPheProAspSerSerMetArgLeuValGluAsnValHisArg900905910AsnHisProlieAlaAspTyrPheAsnGluGlyValAlaGluAlaVal915920925lieAspHisValArgHisArgValAlaArgGluArgGlyValArgLeu930935940ArglieLeuGlulieGlyAlaGlyThrGlyGlyThrSerSerLysVal945950955960LeuProAsnLeuLeuProTrpArgGluGlnlieGluGluTyrArgTyr965970975ThrAspLeuSerLysSerPheLeuPheTyrAlaGluAspThrTyrGly980985990AlaAspAlaProPheLeuLysThrAlalieLeuAsplieGluAlaPro99510001005ThrThrAspAspHisProHisAlaAspLysPheGlySerTyrAspMet101010151020VallieAlaAlaAsnValLeuHisAlaThrArgAsplieGlyArgThr1025103010351040LeuArgHisAlaLysAlaPheMetArgAspGlyGlyValLeuValLeu104510501055AsnGluMetSerGluAsnCysAlaPheSerHisLeuThrPheGlyLeu106010651070LeuGluGlyTrpTrpArgTyrAspAspGluProValArglieProGly107510801085[3262]CysProGlyLeuSerProLeuGluTrpArgArgGlnLeuGluArgAla[3263]109010951100GlyPheValAspValGlnPheProLeuGlyAspAlaGlnProLeuGly1105111011151120GlnGlnVallieValAlaGlnCysArgGlnArgAlaTrpLeuArgAla112511301135GluArgAspGluAlaArgGlyAlaAlaAlaAlaHisArgHisArgAla114011451150TrpLeuArgArgArgAlaArgThrAspThrArgAspAlaAlaArgGly115511601165SerProAlaAspArgProGlyArgCysArgSerArgThrGlnLeuLeu117011751180ArgHisArgThr1185<210>17<211>4904<212>PRT<213>Psuedomonassp.NO.2663<400>1LeuGlylieGlnLeuGlyThrlieGluLeuPheAspHisAsnThrVal151015AlaGlnLeuAlaAlaHisLeuThrAsnLeuHisArgValThrGluAla202530AlaGlyGlySerAlaProAlaProAlaProCysGluThrAlaThrAsn354045AlaAlaAlaGlyProSerSerArgAlaAlaThrGlyAspValLeuGlu505560ArgValThrValMetLeuArgGluHisValAlaArgAlaValArgVal65707580ArgGluAspGlulieAspLeuHisArgSerPheAlaAspGlnGlyVal859095AspSerlielieSerValGlnlieValAsnArglieAsnLysLeuPhe100105110GlyLeuArglieAlaThrThrValMetPheAspHisAsnThrValGly115120125ArgLeuAlaAlaHisLeuCysGluArgHisGlyAlaAlaLeuAlaLys130135140AlaValGlnThrGluAlaSerThrProProAspAlaAlaAlaProAsp145150155160ValAlaProAlaArgAlaAlaAlaThrAlaLeuSerAlaArgGluSer[3302]165170175GluProSerArgThrAlaSerAlaProProAlaAlaGlyProGlySer180185190lieCysAlaValLeulieAspGlyProGlyGlulieGluAspLeuSer195200205lieAlaAlaValAlaSerArgGlyLeuAspHisAspGluValArgVal210215220AlaValArgAlaPheSerLeuAsnPheSerAspLeuLeuCysAlaArg225230235240GlyLeuTyrProAsnMetProProTyrProPheValProGlyAsnGlu245250255AlaAlaGlyValVallieGluValGlyAlaGlyValSerThrPheArg260265270ProGlyAspGluValValCysLeuCysGlnGlyCysHisAlaGluGln275280285VallieCysThrThrSerGlnValPheAlaLysProAlaSerTrpSer290295300PheGluGluAlaCysAlaLeuProlieValGlylieThrMetlieAsp305310315320AlaPheSerLysAlaAlaLeuAlaArgGlyGluArglieLeulieGln325330335ThrAlaThrGlyGlyThrGlyLeulieAlaValGlnLeuAlaMetHis340345350ArgGlyAlaGluliePheAlaThrAlaGlySerGluAlaLysLeuArg355360365TyrLeuArgAspLeuGlylieAlaHisThrlieAsnTyrArgThrGlu370375380AspPheAlaGlnGluLeuAlaArgLeuAlaProGlyGlyValAspVal385390395400VallieAsnThrLeuGlyGlyAspAlaLeuGlnLysGlylieAspSer405410415LeuGlyProGlyGlyArgTyrlieGlulieAlaMetThrSerLeuArg420425430SerAlaArgSerlieAspLeuSerSerLeuSerAspAsnGlnThrPhe435440445TyrSerValAspLeuAlaArgMetGlyAlaArgAspProArgLyslie450455460AlaArgTyrTrpAspGluLeuValSerLeuThrGluGlnGlyValVal465470475480[3341]ArgProThrlieSerGluValValAlaPheArgAspValHisAspAla485490495TyrArgArgLeuAlaAspArgGlyAsnlieGlyLyslieValValArg500505510[3345]ValAspGlnSerSerAlaProArgProArgGluArgGluArgGlyAla515520525AsnLeuProAlaArgAspAlaAlaAlaSerGlyAlaProAspGlyAla530535540ThrGluProAlaGlyAlaGlyAlaArgValValArgAspAspAsplie545550555560AlalielieGlyMetSerGlyArgPheAlaArgSerProAspLeuAsp565570575AlaPheTrpSerHisLeuAlaSerGlyThrSerLeulieAlaProVal580585590ThrArgTrpAspLeuGlyAspGlyAspAlaArgCysGluThrGlySer595600605PheLeuAspAspLeuSerThrPheAspProPhePhePheLyslieSer610615620GlyThrGluAlaArgTyrMetAspProGlnGlnArgLeuPheLeuGlu625630635640GluAlaTrpLysAlaLeuGluAspAlaGlyHisAlaGlyGluLeuAsp645650655LysGlnArgArgCysGlyValTyrValGlyCysSerGlyGlyAspTyr660665670HisLeuLeuPheProGlyProGlyAlaSerAspGlyAspThrArgAla675680685ArgProProlieGlnSerTyrTrpGlyAsnAlaValSerValliePro690695700AlaArglieSerTyrPheLeuAspLeuHisGlyProAlaValAlalie705710715720AspThrAlaCysSerSerSerLeuValAlaLeuHisMetAlaCysAla725730735AlaLeuArgSerGlyGluValSerMetAlaLeuCysGlyGlyValPhe740745750ValGlnCysThrAlaGlyPheTyrGlnGlnAlaGlnArgAlaGlyMet755760765LeuSerThrThrGlyAsnCysTyrAlaPheAspAlaArgAlaAspGly770775780PheValProGlyGluGlyValGlyAlalieValLeuLysArgLeuGly[3380]785790795800AspAlalieAlaAspGlyAspHislieAspGlyVallieLeuAlaSer805810815GlylieAsnGlnAspGlySerThrAsnGlylieThrAlaProSerAla820825830ArgSerGlnGluGluLeuGluThrAspValTyrAspArgPheGlylie835840845AspProArgGluLeuGlnMetValGluAlaHisGlyThrGlyThrPro850855860LeuGlyAspProlieGluPheThrAlaLeuAlaAsnAlaPheGlyArg865870875880TyrThrArgAspArgGlyPheCysAlaLeuGlySerValLysThrAsn885890895lieGlyHisThrThrThrThrAlaGlylieAlaGlyValLeuLyslie900905910LeuLeuAlaLeuArgHisArgGlnLeuProProSerCysAsnPheGlu915920925ArgProAsnProGlulieGluLeuAspAspSerProPheTyrValAsn930935940ThrArgLeuArgProTrpArgValGluThrGlyArgArgArgMetAla945950955960AlaValSerSerPheGlyPheSerGlyThrAsnAlaHisValVallie965970975AlaGluAlaProProlieAlaLeuGlyAsnProArgSerAlaProGly980985990GlnProGluLeuLeuValValSerAlaGlnThrHisAlaGlnLeuAsn99510001005AlaGlnLeuGluArgMetAlaAlaPheAlaSerGlyGlyAlaAlaArg101010151020AspLeuSerLeuAlaAsplieAlaPheThrLeuAlaAspGlyArgArg1025103010351040HisMetArgTyrArgTrpAlaCysValAlaSerSerValGluGluPhe104510501055AlaAlaTyrCysGlyAlaGlyValProSerSerProSerThrSerThr106010651070AlaValPheGlnGlyAspAlaAsnGluAlaLeuSerProHisValAla107510801085AspArgValGlyAspAlaAspLeulieSerGlnAlaArgArgPheVal109010951100[3419]AlaGlyGlyArgLeuAspSerAlaArgTrpThrGlyArgGlyCysArg1105111011151120ArgValArgLeuProValTyrProPheAlaArgGluSerTyrTrpVal112511301135GluThrProGlyGluGluArgAsnProTrpProProLeuGluValAla114011451150GluAlaGlylieGlyProValTrpArgPheSerArgArgLeuGluGly115511601165SerGluProPheLeuArgAspHisArgValLeuGlyGluSerTrpLeu117011751180ProAlaValValTyrTrpGluMetThrArgTrpSerAlaArgGlnAl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laGluGlnValTyrArgPheLeuAlaAlaArgGly210215220PheGluTyrGlyProArgGlnArgAlaLeuGlnArgAlaThrLeuHis225230235240SerProGluCysLeuTyrAlaAspLeuAlaLeuProValProLeuVal245250255SerSerLeuProThrMetMetLeuHisProSerLeuLeuAspGlyAla260265270LeuGlnAlaAlaAlaGlyPheTyrLeuAlaAspAlaAlaAsplieGlu275280285ProGlyLeuProPheAlalieAspGlulieAlaValTrpArgValGly290295300GluProSerMetArgAlaArgValThrArgArgThrThrGluValGly305310315320AlaValThrArgLeuAsplieGluLeuTyrAspAlaAspGlyArgLeu325330335CysAlaAlaLeuLysGlyLeuThrSerArgValGlyArglieArgAla340345350ArgHisGlySerGlyLeuLeuLeuAlaArgProGluTrpArgAlaGlu355360365ProPheValValGlyAlaArgAlaAlaArgGlnProAlaProCysArg370375380lieLeuLeuValGlyAspLeuGlyLysValGlyGluValAspAlaLeu385390395400AlaAlaSerLeuArgThrArgTrpProAlaGluLeuAlaGlyProGlu405410415lieValArglieAlaPheAspProValSerAlaGlnAspAspProlie420425430GluSerSerLeuArgLeuLysHisCysLeuGlnAspTrpSerAlalie435440445ProCysArgGluSerGluLeuTrpLeuValAlaMetAspAspAlaSer450455460ProLeuAlaArgSerMetHisAlaMetLeuLysCyslieAlaAlaGlu465470475480AspProArgArgAlaThrArgLeuValSerCysThrAlaArgAlaAsp485490495ValAsnGlulieAlaGlyAlaLeuValAspGluAlaArgAlaAspAsp500505510AspValAspCysValArgTyrAlaGlyValArgGluValLysArgTrp[3965]515520525ValSerValAlaAlaGluAlaAsnAlaProMetHisGlyTrpArgAsp530535540GlyGlyArgTyrLeulieSerGlyGlyThrGlyHisLeuGlyLeuAla545550555560PheAlaGlnAsplieAlaAlaSerValThrGlyAlaCysliePheLeu565570575LeuGlyArgHisGlyProAspAlaLeuAspAlaArgGlnGlnThrArg580585590LeuArgGlulieAlaAlaAlaGlyAlaArgValMetHisCysArgAla595600605AspValAlaAspAlaGluAlaValAlaGluThrlieAlaArglieGlu610615620ArgGluHisGlyProLeuHisGlyValLeuHisAlaAlaGlyValAla625630635640AspAspGlyLeuLeuValAsnLysThrAspAspThrTrpArgArgVal645650655MetArgProLysValLeuGlyAlaAlaAsnLeuAspArgAlaThrArg660665670HisMetProLeuAspCysPheValLeuPheSerSerGlySerAlaVal675680685PheGlyAsnValGlyGlnSerAspTyrAlaAlaAlaAsnAlaTyrLeu690695700AlaGlyTyrAlaArgTrpArgGlnAlaGluValGlylieGlyAlaAla705710715720HisGlyArgSerLeuCysLeuHisTrpProLeuTrpAspGlyGlyMet725730735AlaAlaGlylieAlaAspProGlnArgLeuTyrHisAlaThrGlyMet740745750ArgProMetAspAlaValLeuGlylieGluAlaPheArgArgAlaLeu755760765AlaThrProCysThrGluLeuLeuValAlaAlaGlyAspLeuAspArg770775780LeuLysArgTyrLeuThrGlyValAlaAspThrAspAlaAlaGlyAla785790795800AspGluAlaAlaAlaProProAlaProAspLeuSerGlnValAspSer805810815GluAlaLeuHisGlyAlaAlaValArgLeuLeuArgArgValValGly820825830[4004]GlyValLeuLysLeuGlnValGluGlnMetArgAlaAspGlnProLeu835840845AspGlnTyrGlylieAspSerValAspMetMetLysAlaThrArgGlu850855860LeuGluGluThrPheGlyThrLeuProLysThrLeuPhePheGlnTyr865870875880ProSerlieAsnGluLeuAlaGlyHisPheAlaGluHisHisAlaGlu885890895AlaLeuAlaAlaThrGlyThrProValAlaProAlaSerGlyProArg900905910AlaThrGlnValProMetThrGlnProlieSerHisProAlaGluMet915920925ProArgProThrProGlyValValAspAlaSerCysValSerGlyAsp930935940AlaAlaArgAlaAsplieAlalielieGlyLeuSerGlyArgTyrPro945950955960GlnAlaAlaAspValValGluPheTrpAspAsnLeuAlaGluGlyVal965970975AspAlalieArgGlulieProProGluArgTrpAspTrpArgSerPhe980985990TyrArgGluAspGlySerArgArgProGlyThrHisAlaSerArgTrp99510001005GlyGlyPhelieAspGlyValAspGluPheAspProLeuPhePheSer101010151020lieSerProSerGluAlaGlulieMetAspProGlnGluArgLeuPhe1025103010351040LeuGlnHisAlaTrpAlaAlaLeuGluAspAlaGlyTyrSerArgGly104510501055ArgLeuAlaArgAlaArgArgGluArgGluAlaAlaGlyAspGluAla106010651070ArgMetAspGlulieGlyValTyrValGlyValMetTyrSerGluTyr107510801085LeuGlnCysAlaAspAsnAspProGluArgLeuValGlySerSerTyr109010951100AlaSerlieAlaAsnArgValSerTyrAlaLeuAspLeuArgGlyPro1105111011151120SerLeuAlalieAspThrMetCysSerSerSerLeuThrCysLeuHis112511301135LeuAlaCysArgGluLeuAlaSerGlyGlylieGlyMetAlaLeuVal[4043]114011451150GlyGlyValAsnLeuAsnLeuHisProAsnLysTyrHisLeuLeuSer115511601165GlnGlyArgPheValSerProArgGlyArgCysHisSerPheGlyAla117011751180AspGlyAspGlyTyrValProSerGluGlyValGlyValValCysVal1185119011951200LysArgLeuAlaAspAlaGluArgAspGlyAspProlieTyrAlaVal120512101215lieArgGlySerAlalieAsnHisGlyGlyLysSerGlnGlyTyrSer122012251230ValProGlnProArgArgGlnGlnAspVallieArgGlnAlaLeuThr123512401245GlnAlaGlnValAsnAlaArgSerValSerTyrValGluAlaHisGly125012551260ThrGlyThrAlaLeuGlyAspProlieGlulieAlaGlylieAlaGly1265127012751280AlaPheSerArgAspThrAlaAspThrGlnPheCysAlalieGlySer128512901295ValLysSerSerValGlyHisCysGluAlaAlaAlaGlyMetAlaAla130013051310LeuThrLysVallieGlyGlnMetArgAlaGlyGluLeuPheProSer131513201325LeuHisAlaGlnArgLeuAsnProAsnlieAspPheAlaAlaSerPro133013351340PheValValValArgAspArgGlnProTrpLysGlyAlaThrGlySer1345135013551360ArglieAlaAlaValSerSerPheGlyAlaGlyGlySerAsnAlaHis136513701375PheValLeuGlyAspTyrAlaArgProAlaAspAspAlalieAspGly138013851390GluAspGlyGlylieCysLeuValProLeuSerAlaArgThrGlnGlu139514001405ArgLeuAlaGluGlnAlaArgAlaLeuAlaAlaTyrLeuSerAspCys141014151420AlaArgProGlyAlaGlyGlyArgHisTrpArgHisProProAlaGly1425143014351440LeuAlaAsnlieAlaTyrThrLeuGlnThrGlyArgGluProTrpGlu144514501455[4082]AlaArgValAlaPhelieAlaThrSerArgAspGluLeuLeuAlaLeu146014651470LeuAsnGlyTyrLeuAspGlyArgProAspAlaHisSerValPheSer147514801485GlyThrArgAlaAsnGlyAsnArgLeuLeuProLeuLeuGlyMetAsp149014951500[4088]GlnAspSerHisAlaLeulieAspThrTrpPheAlaLysArgLysTyr1505151015151520AlaGluLeuLeuThrValTrpValGluGlyValAspPheAspTrpGlu152515301535ArgLeuTyrAlaSerAspGlyAlaGlyGlyArgArgProArgArgVal154015451550AsnLeuProThrTyrProPheAlaArgGluArgTyrTrpLeuProThr155515601565ProAspValGluAlaProArgAlaGluProAsnArgLysAlaMetThr157015751580AsnLeuThrThrThrProArgValGlyTyrArgValAlaValTrpGlu1585159015951600ArgLeuProMetGlyAlaGluAlaSerHisAlaValThrAspValLeu160516101615LeuCysGlyTrpAspAlaAlaGluAlaGlnArgPheAlaAlaHisTrp162016251630ProAlaHisArgCysArglieValGlySerAlaAlaAspThrAspAla163516401645lieAspGlyAlaPheThrGlnLeuAlaLeuAlaValPheSerAspLeu165016551660GlnAlaArgLeuAlaThrAlaAlaGlyAlaAsnValArgAlaGlnLeu1665167016751680LeuValProProGlyAspArgGlyArgLeuProSerAlaLeuGluAla168516901695MetLeuArgSerLeuMetLeuGluHisProThrPheAlaGlyGlnLeu170017051710ValGlnCysAspAspValSerSerAspAlaAlaLeuArgGluAlaAla171517201725GlyLeuAlaAlaHisHisProArgAlaThrLeuValArgTyrHisGlu173017351740GlyAlaLeuSerGlnArgValTrpArgGluAlaAspGlyAspGluPro1745175017551760GlyGluSerProTrpArgGluGlyGlyValTyrLeulieThrGlyGly[4121]176517701775[4122]GlyGlyAlaLeuGlyThrLeuLeuAlaArgGlnLeuAlaGlyGluLeu178017851790SerSerAlaThrLeulieLeuThrGlyArgArgAlaAlaSerGluLeu179518001805ThrGlyAlaProValGlySerLeuLeuAspThrLeuArgAlaGlnGly181018151820lieAlalieGluTyrArgAlaAlaAsplieAlaAspLeuAlaAlaCys1825183018351840GluGlnLeuLeuProAsplieValArgGluHisGlyAlaLeuAsnGly184518501855ValPheHisAlaAlaGlyValSerArgAspGlyAlaAlaLeuArgLys186018651870ThrAlaGlnThrPheArgGluValLeuAlaProLysValAlaGlyThr187518801885ValAsnLeuAspCysAlaThrArgHisLeuProLeuAspPheLeuVal189018951900LeuTyrSerSerLeuSerGlyAlaMetGlyAsnAlaGlyGlnCysAsp1905191019151920TyrAlaThrAlaAsnAlaPheLeuAspArgTyrAlaAlaHisArgAla192519301935ValLeuAlaAlaThrGlyAlaCysHisGlyArgThrValSerlieGly194019451950TrpProTrpTrpGluGlyGluGlyMetAlaProAspAlaHisAlaPro195519601965GlyValPheValSerProArgGlyArgCysHisSerPheGlyAlaAsp197019751980GlyAspGlyTyrValProSerGluGlyValGlyValValCysValLys1985199019952000ArgLeuAlaAspAlaGluArgAspGlyAspProlieTyrAlaVallie200520102015ArgGlySerAlalieAsnHisGlyGlyLysSerGlnGlyTyrSerVal202020252030ProGlnProArgArgGlnGlnAspVallieArgGlnAlaLeuThrGln203520402045AlaGlnValAsnAlaArgSerValSerTyrValGluAlaHisGlyThr205020552060GlyThrAlaLeuGlyAspProlieGlulieAlaGlylieAlaGlyAla2065207020752080[4160]PheSerArgAspThrAlaAspThrGlnPheCysAlalieGlySerVal208520902095LysSerSerValGlyHisCysGluAlaAlaAlaGlyMetAlaAlaLeu210021052110ThrLysVallieGlyGlnMetArgAlaGlyGluLeuPheProSerLeu211521202125HisAlaGlnArgLeuAsnProAsnlieAspPheAlaAlaSerProPhe213021352140ValValValArgAspArgGlnProTrpLysGlyAlaThrGlySerArg2145215021552160lieAlaAlaValSerSerPheGlyAlaGlyGlySerAsnAlaHisPhe216521702175ValLeuGlyAspTyrAlaArgProAlaAspAspAlalieAspGlyGlu218021852190AspGlyGlylieCysLeuValProLeuSerAlaArgThrGlnGluArg219522002205LeuAlaGluGlnAlaArgAlaLeuAlaAlaTyrLeuSerAspCysAla221022152220ArgProGlyAlaGlyGlyArgHisTrpArgHisProProAlaGlyLeu2225223022352240AlaAsnlieAlaTyrThrLeuGlnThrGlyArgGluProTrpGluAla224522502255ArgValAlaPhelieAlaThrSerArgAspGluLeuLeuAlaLeuLeu226022652270AsnGlyTyrLeuAspGlyArgProAspAlaHisSerValPheSerGly227522802285ThrArgAlaAsnGlyAsnArgLeuLeuProLeuLeuGlyMetAspGln229022952300AspSerHisAlaLeulieAspThrTrpPheAlaLysArgLysTyrAla2305231023152320GluLeuLeuThrValTrpValGluGlyValAspPheAspTrpGluArg232523302335LeuTyrAlaSerAspGlyAlaGlyGlyArgArgProArgArgValAsn234023452350[4194]LeuProThrTyrProPheAlaArgGluArgTyrTrpLeuProThrPro235523602365AspValGluAlaProArgAlaGluProAsnArgLysAlaMetThrAsn237023752380LeuThrThrThrProArgValGlyTyrArgValAlaValTrpGluArg[4199]2385239023952400LeuProMetGlyAlaGluAlaSerHisAlaValThrAspValLeuLeu240524102415CysGlyTrpAspAlaAlaGluAlaGlnArgPheAlaAlaHisTrpPro242024252430AlaHisArgCysArglieValGlySerAlaAlaAspThrAspAlalie243524402445AspGlyAlaPheThrGlnLeuAlaLeuAlaValPheSerAspLeuGln245024552460AlaArgLeuAlaThrAlaAlaGlyAlaAsnValArgAlaGlnLeuLeu2465247024752480ValProProGlyAspArgGlyArgLeuProSerAlaLeuGluAlaMet248524902495LeuArgSerLeuMetLeuGluHisProThrPheAlaGlyGlnLeuVal250025052510GlnCysAspAspValSerSerAspAlaAlaLeuArgGluAlaAlaGly251525202525LeuAlaAlaHisHisProArgAlaThrLeuValArgTyrHisGluGly253025352540AlaLeuSerGlnArgValTrpArgGluAlaAspGlyAspGluProGly2545255025552560GluSerProTrpArgGluGlyGlyValTyrLeulieThrGlyGlyGly256525702575GlyAlaLeuGlyThrLeuLeuAlaArgGlnLeuAlaGlyGluLeuSer258025852590SerAlaThrLeulieLeuThrGlyArgArgAlaAlaSerGluLeuThr259526002605GlyAlaProValGlySerLeuLeuAspThrLeuArgAlaGlnGlylie261026152620AlalieGluTyrArgAlaAlaAsplieAlaAspLeuAlaAlaCysGlu2625263026352640GlnLeuValAlaAsplieValArgGluHisGlyAlaLeuAsnGlyVal264526502655PheHisAlaAlaGlyValSerArgAspGlyAlaAlaLeuArgLysThr266026652670[4234]AlaGlnThrPheArgGluValLeuAlaProLysValAlaGlyThrVal267526802685AsnLeuAspCysAlaThrArgHisLeuProLeuAspPheLeuValLeu269026952700[4238]TyrSerSerLeuSerGlyAlaMetGlyAsnAlaGlyGlnCysAspTyr[4239]2705271027152720AlaThrAlaAsnAlaPheLeuAspArgTyrAlaAlaHisArgAlaVal272527302735LeuAlaAlaThrGlyAlaCysHisGlyArgThrValSerlieGlyTrp274027452750ProTrpTrpGluGlyGluGlyMetAlaProAspAlaHisAlaAlaArg275527602765ValLeuArgGluThrGlyMetLeuProLeuGlyAlaAspAspGlyMet277027752780ArgAlaLeuLysArgValLeuValHisGlyGlyGluHisValLeuVal2785279027952800ValHisGlyAspAlaAlaArgMetArgAlaAlaAlaGluGlnAlaGlu280528102815AlaAlaAspGlyProThrAlaSerAlaSerAlaGlyArgGlySerArg282028252830SerThrAlaArgAspArgValGlyLeuAspGluArgValThrAspArg283528402845LeuArgGluLeuLeuGlyArgLeuCysLysLeuSerProGlnArglie285028552860AspProHisValArgLeuGluGlnTyrGlylieAspSerValArglie2865287028752880ValHisPheGlnGlnAlaLeuGlyGluProTyrProGlylieSerSer288528902895AlaMetLeuTyrGluHisThrThrlieAlaSerLeuAlaAlaHisLeu290029052910ValAlaAlaTyrProSerGlnSerArgAlaTrpAlaGlyGluProGlu291529202925AspProAlaAlaSerProValGluThrAlaLeuAspGlyTyrGlySer293029352940AspAlaAlaGlnAlaLeuAlaProGlnArgGlnArgGluHisAspArg2945295029552960ArgArgArgSerGlyProGlyArgArgGlySerHisArgAspHisTrp296529702975HisArgGlyProLeuSerGluGlyCysGluProCysArglieLeuAla298029852990GlnSerArgGlyArgArgArgLeuTyrArgArgAspSerAlaArgThr299530003005LeuValAlaArgArgPheLeuArgArg[4277]30103015<210>19<211>2662<212>PRT<213>Psuedomonassp.NO.2663<400>1MetAspProGlnGluArgLeuPheLeuGluCysAlaTrpGluAlaLeu151015GluAspAlaGlyTyrSerArgGluMetLeuValGluArgHisArgAla202530ArgAlaGlyValPheValGlyAlaThrLysThrGlyTyrGluLeuTyr354045GlyProGluPheTrpArgGlnAspLysValPheLeuProArgThrSer505560PheGlySerlieAlaAsnArgValSerTyrCysLeuAspLeuMetGly65707580ProSerLeuAlalieAspThrMetCysSerSerSerLeuValAlalie859095HisGluAlaCysGluArgLeuArgArgGlyAspCysAsplieAlalie100105110AlaGlyGlyValAsnLeuTyrLeuHisProAlaAsnTyrValAlaLeu115120125CysSerGlyHisMetLeuSerAlaGluGlyArgCysArgSerPheGly130135140AlaGlyAlaAspGlyPheValProGlyGluGlyValGlyAlaVallie145150155160LeuLysProLeuGlyGlnAlaArgArgAspGlyAspAlalieHisAla165170175VallieArgAlaSerGlylieAsnHisSerGlyLysThrHisGlyTyr180185190MetValProSerProAlaSerGlnGlnAlaLeulieArgAspThrLeu195200205AlaArgAlaArgCysAspAlaAlaSerlieGlyTyrLeuGluAlaHis210215220GlyThrGlyThrThrLeuGlyAspProlieGluPheArgGlyLeuVal225230235240GlnAlaPheGlyGlyAlaArgAlaAlaGlyValHisCysAlaLeuGly[4314]245250255SerValLysSerAsnlieGlyHisLeuGluAlaAlaAlaGlyMetAla[4316]260265270AlaLeuThrLysValValLeuGlnLeuArgHisArgLysLeuValPro275280285ThrLeuHisAlaAspSerLeuAsnProGlulieAlaLeuAspGlySer290295300[4321]GlyPheTyrLeuSerAspThrLeuArgAsnTrpMetProGlnProGly305310315320SerAspAlaGlnLeuArgAlaGlylieSerSerPheGlyAlaGlyGly325330335ValAsnAlaHisLeulieValGluGlnTyrArgAspGlyAspGluGly340345350ProAspArgGlyAlaThrArgLeuAspThrAlaThrValGluGluPro355360365AlaValPheValLeuSerAlaArgAsnThrGlyArgLeuAspAlaTyr370375380LeuAspArgVallieAlaPheLeuAlaAsnGlyGlyGlyProLeuAla385390395400AspLeuCysArgMetMetGlnThrGlyArgSerAlaMetAlaGluArg405410415CysAlaValValCysArgSerThrAlaGluLeuLeuAsplieLeuArg420425430MetHisArgArgGlyGluArgValGluGlyPheHisArgAlaArglie435440445AspGlyThrGlyValAlaAlaGluAlaProAlaLeuAspAspLeuAsp450455460AlaCysAlaArgAlaTrpValSerGlyValAsplieAspTrpArgSer465470475480ArgArgAlaAlaArgArgAlaArgGlnAlaArgAlalieAlaLeuPro485490495ThrTyrProPheGlnProArgArgPheTrpLeuAspThrLeuAlaPro500505510AlaLeuAlaLeuGluValAspSerGluGlyThrProGlyGlyAlaGly515520525ValProlieMetAsnGlyGlyGlyProThrAspAlalieMetGlyLys530535540AlaProAlaHisArgValGluValAlaSerGluAlaGlyAspAlaThr545550555560SerValLeuProArgAlaSerHisAlaProThrProSerGluGlyThr565570575[4355]SerAlaGlyThrlieArgLeuProAlaProSerArgPheValAlaSer[4356]580585590SerGluAlaAlaSerThrMetProlieThrLeuAlaSerProSerGlu595600605ValValArgAlaArgSerAlaThrMetProValProPheAspThrGly610615620AlaValAspGluAlaLeuValAlaArgCysAspAspValHisProSer625630635640LeuHisHisValAspLeuProValGlyLeuAspAlaAlaAspLeuVal645650655AlaArgLeuThrAlaLeuPheGluArgProThrTrpGlyGluProCys660665670AspAlaValLeuLeuHisSerProArgAspGlyLeuArgLeuSerLeu675680685ValGlyValProAspAspAlaLeuAlaArgLeuValAspAlalieGly690695700GlnAlaProSerValValLeuAlaAlaLeuHisGlyAspAlalieAsp705710715720LeuGlyTrpVallieGlyAlaAlaCysAspLeuMetlieValAsnAla725730735GluSerArgTyrGlyCysProSerThrSerSerValValAlaArgAsp740745750LeuLeuArgAlaArgPheGlyAlaArgProGlySerAlaAlaMetLeu755760765SerGlyGluAlaArgThrGlyAlaAlaTrpArgAlaThrAsnLeuGly770775780LeuHisAlaValProAlaLeuAlaValGlyGluArgAlaArgAlaAla785790795800MetGluThrlieValThrGluGlyGlyArgAlaAlaGlyLeuLeuLys805810815AlaHisTrpArgGlyThrPhePheAlaGluAlaAsnThrLeuGlyAla820825830ProSerSerTrpProValLeuAlaGluProGluArgGluProAlaArg835840845AlaAlaAlaAlaAlaAlaAlaGlySerGluArgProValProLeuPro850855860HisThrCyslieGluLeuThrLeuAspAspLeuGlyValAlaValAla865870875880ArgMetCysAspProAspThrLysAsnAlaPheThrProAlaPheVal[4394]885890895AlaAlaMetGluAlaValMetThrTrpAlaGlyGluThrProAlaCys900905910LysValLeulieLeuThrGlyTyrGlyHisTyrPheAlaThrGlyGly915920925ThrArgGlnGlyMetGlnAlalieGlnGlnGlyAsnAlaThrPheThr930935940AspAlaArgLeuTyrGluLeuProLeuAlaCysProValProVallie945950955960AlaAlaMetGlnGlyHisAlalieGlyAlaGlyTrpSerMetGlyMet965970975ValCysAspAlaValLeuPheAlaGluGluSerValTyrHisSerPro980985990TyrLeuSerTyrGlyPheThrProGlyAlaGlySerThrLeuValPhe99510001005ProMetArgLeuGlyLeuAspLeuGlyArgGlulieLeuPheGlyAla101010151020GlnProTyrLysGlyArgAlaLeuArgGluArgLeuProGlyLeuSer1025103010351040ValValProArgGlyGluValLeuAlaGlnAlaArgArgLeuAlaAla104510501055GlnTrpAlaMetArgArgThrArgAspAlaLeulieArgAspLysGly106010651070ArgArgLeuAlaAlaLeuAlaGlnAlaLeuProAlaMetlieArgLys107510801085GluLeuAspMetHisGluLeuThrPheArgHisAsnProAspValAla109010951100ArgLeulieAspArgAlaTyrGlyPheAlaSerThrAlaAspAlaAsp1105111011151120ThrAspAlaGlySerArgCysAlaProGlyGlnAspLeulieThrLeu112511301135LeuLysSerThrLeuAlaThrGlulieGlnMetSerSerAspGluLeu114011451150AspAspGlnAlaSerPheValAspLeuGlyLeuAspSerValThrAla115511601165ValThrTrpThrArgThrLeuGlyArgAlaLeuArgValGluLeuThr117011751180ProAlaHisValTyrGlnTyrProSerlieAlaLysLeuLeuAlaTyr1185119011951200[4433]LeuArgGluAlaThrAlaGlySerAlaSerGluValAlaProValThr120512101215AlaGlnValGlnProGlnProAlaThrValGlyAlalieAlaGluVal122012251230ValArgCysThrLeuAlaAlaGluLeuGlnLeuAspGluGlnAlalie123512401245AspAlaGlnAlaSerPheValAspLeuGlyLeuAspSerValThrAla125012551260VallieTrpAlaArgLysLeuHisGluGlnLeuSerValAlaLeuSer1265127012751280ProThrGlnLeuTyrGlnTyrProAsnLeuAlaAlaLeuCysAlaTyr128512901295LeuGlyGlyGluAsnThrAlaAsnAlaAlaGluLysThrGlyGluGly130013051310ValAlaProThrHisThrGlyLeuSerValSerValArgAlaMetAla131513201325AspGlyArgAlaProValArgLeuProArgValAspGluGluArgPro133013351340ThrAlaProValAspArgAspSerValAlaHisSerLeuAlaHisGlu1345135013551360LeuGlnLeuAspValThrGlulieAspProArgThrSerPheValAsp136513701375MetGlyLeuAspSerValThrAlalieThrTrpSerLysAlaLeuThr138013851390ValAlaHisGlyValGluValArgProThrAspLeuTyrArgTyrPro139514001405ThrLeuAspAlaLeuValValHisLeuSerArgAlaProArglieAla141014151420ProValArgAlaAlaThrSerAlaSerMetThrGluGlyProAlaGly1425143014351440SerProValThrArgGlyGlnArgGlyProAlaAlaAlaProProPro144514501455MetProAlaAlaProLeuArgAspAlaSerArgGluArgValAsplie146014651470AlalielieGlyMetAlaGlyArgPheProGlyAlaAlaAspLeuAsp147514801485AlaPheTrpArgAsnLeuValGluGlyArgAspSerlieValGluVal149014951500ProProHisArgTrpSerLeuAspArgTyrTyrAspProAsnProGln[4472]1505151015151520[4473]SerLysGlyLysThrTyrSerArgTrplieGlyLeuLeuAspAspAla152515301535AspAlaPheAspAlaGluPhePheGlylieSerProArgGluAlaGlu154015451550HisMetAspProGlnGlnArgValPheMetGluAsnCysTrpHisAla155515601565lieGluHisAlaGlyTyrAsnProPheAsnLeuAlaThrArgArgThr157015751580GlyLeuPheValGlyCysAlaThrGlyAspTyrAlaAspLeuGlyGln1585159015951600ArgAspGluAlaAsnAlaGlnSerPheMetGlyGlyAlaSerSerlie160516101615LeuSerAlaArgMetProTyrPheLeuAsnLeuAspGlyProCysLeu162016251630AlalieAspThrAlaCysSerSerAlaLeuValAlaValAsnGluAla163516401645CysAsnSerLeuLeuLeuGlyAsnCysAspPheAlalieAlaGlyGly165016551660ValCysValMetAlaGlyProThrLeuHislieLeuSerSerLysAla1665167016751680GlyMetLeuSerProThrGlyArgCysArgThrPheAspGluAlaAla168516901695AspGlyPheValProGlyGluGlyValGlyThrValLeuLeuArgArg170017051710LeuAspAspAlaLeuAlaAlaGlyAspThrlieHisGlyVallieArg171517201725GlyTrpGlylieAsnGlnAspGlyArgThrAsnGlylieThrAlaPro173017351740AsnProAspAlaGlnArgArgLeuAlaThrGlylieTyrArgArgPhe1745175017551760GlylieAspProLysArglieThrLeuAlaGluAlaHisGlyThrGly176517701775ThrArgLeuGlyAspProlieGluValArgAlaLeuThrAspAlaPhe178017851790HisGluPheAspValGlyAsnAlaTyrCysAlalieGlySerValLys179518001805SerAsnValGlyHisLeuAlaLeuAlaAlaGlylieAlaGlyLeuLeu181018151820[4511]LysValValLeuSerLeuArgHisArgThrlieProProThrLeuHis1825183018351840CysAspThrValAsnGluHislieAspPheAlaArgSerProPheHis184518501855ValProArgValAlaGlnAlaTrpSerAlaAlaAspGlyGluProArg186018651870MetAlaCysValSerSerPheGlyPheSerGlyThrAsnAlaHisLeu187518801885ValValGluGluAlaHisAlaValAlaProProAlalieGlyAspGln189018951900SerAlaArgGluHisLeuValValLeuSerAlaArgArgProGluSer1905191019151920LeuArgAlaAlaAlaLysArgLeuArgAspAlaLeuAlaAlaGlnAsp192519301935AlaAlaGlySerAlaProArgLeulieAspValAlaTyrThrLeuHis194019451950ThrGlyArgAlaThrPheArgHisArgLeuAlalieValAlaSerSer195519601965LeuAspGlyLeulieGluArgLeuAspGlyTrpLeuAlaSerGlyPro197019751980AspAspGlnAlaGlyValHisAspGlyThrAlaProThrSerHisSer1985199019952000AlaGluHisSerAlaArgValAlaGlnAlaLeuAlaAspGlyAspPro200520102015ArgAlaAlaAlaAlaLeuTrpValValGlyGlyGluAlaGlyGlyGlu202020252030ThrArgAlaPheAspGlyLeuSerProArgArglieAlaLeuProGly203520402045TyrValPheValThrSerArgTyrTrpValGlyGlyAlaProLeuAla205020552060AlaGlyAlaProArgProAlaAlaSerGluSerGlnAlaThrSerlie2065207020752080AlaArgGlySerGlyLysArglieAlaValValGlyAlaGlyProAla208520902095GlyLeuValMetAlaLysSerLeuLeuGluGluGlyHisArgProAsp210021052110ValPheGluArgGlnAlaAspLeuGlyGlyValTrpLeuLeuArgAla211521202125GluAsnLysArgAlaGlyAlaTyrArgLysThrArgPheGlnThrSer[4550]213021352140LysTyrThrSerAlaPheSerAspPheAspGlyAlaProlieAspAla2145215021552160[4553]HisPheHisGlyValAlaAsplieHisArgTyrLeuArgAspTyrAla216521702175GluArgPheGlyValThrAlaArglieArgTyrArgThrGluValSer218021852190ArglieGluProHisGlyAspGlnTrpHisValThrThrValCysAsp219522002205GlyAlaSerArgThrAspValTyrAspGlyValAlaLeuCysGlnGly221022152220LeuTyrTrpLysProTrpArgProGluPheAlaGlyLeuAspThrPhe2225223022352240ArgGlyThrlieLeuHisSerAlaGluTyrValAspGluAlaCysLeu224522502255LysGlyLysArgValLeuValValGlyAsnGlylieSerGlyMetAsp226022652270lieAlaGluGluAlaThrGlnSerAlaSerGlnValPheTrpValMet227522802285ArgArgProLysPhelieMetProArgMetThrGlyPheValProAsn229022952300AspPheGlnSerProAlaAsnLeuLeuAlaAsnValAspProAlaGln2305231023152320lieMetGluArgValArgHisSerMetProGluTyrPheArgArgTyr232523302335GlnGluSerGlyLeuLeuProAspAlaAspAspPheArgArgGlnPro234023452350ValValGlnlieAsnAspGlylieValAlaLeuValAlaSerGlyArg235523602365ValGluAlaVallieAspGlulieGluAlaPheAspThrHisGlyCys237023752380ArgPheArgSerGlyArgArglieGluValAspAlaValValPheCys2385239023952400ThrGlyTyrGlnlieAspGluArgPheAspTyrLeuProGlyValSer240524102415MetArgAlaAspPheAlaMetGlyliePheHisArgGluArgProSer242024252430LeuValSerThrSerCysProLeuProValAlaTyrSerGlyThrPhe243524402445[4589]PhePheProGluMetValAlaArgTrpTyrAlaArgValMetSerGly[4590]245024552460ArgSerGluLeuAlaAlaSerGluLeuAspTyrArgLeuAspProArg2465247024752480HisAlaAlalieAsnGlyProlieAlaAsnValValPheGlyLeuArg248524902495LeuGlyLeuLeuProAspProAlaArgGluPheArgAlaPheTrpGln250025052510LeuLeuAsnLeuProProPheProProlieTyrArgLeuArgGlyGlu251525202525HisSerAspProAspAlaProAlaArgLeuGlnAlaLeuAsnArgArg253025352540AsnLeuThrSerGlyLysAlaAspAlaAlaLeuAspValValArgTyr2545255025552560ArgLeuLeuAlaGlyLeuGlyAspAlaThrLeuArgAspLeuLeuAla256525702575ArgGlyGlulieAspAspAsnAspTyrArgAsnAlaGlyArgHisAla258025852590AlaAspAlalieThrLeuAspTrpArgArgGlnTyrlieLeuArgAsp259526002605AspAspValAlaGlnAlaArgArgGluProAlaAlaArgSerGluArg261026152620AlaProHisProGlySerProAlaTrpAsnAspTyrGlnThrLeuLeu2625263026352640AlaArgLeuArgAlaGlyGluLeuAspMetGluGlyValLeuHisAla264526502655LeuThrLysGlnAspAsp2660<210>20<211>3052<212>PRT<213>Psuedomonassp.NO.2663<400>1MetThrGluAsnPheGluAsnTrpPheAspArgLeuArgSerAspThr151015LeuAspGluArgAspGlylieAlaLeuLeuAlaSerLeuArgAspAla202530LeuArgGlySerGlyAlaArgAlaAlaAlaValProGlyAspArgLeu354045[4628]AspGlyAlaArgLeuProArgPheAlaThrProAspTrpGlnAlaArg505560SerTyrArgGluGluArgGlnValAlaProThrPheAspAlaValLeu65707580ValValGlyGluAlaSerLeuAlaAlaAspTrpArgGluGlyLeuThr859095AlaAlaPheSerProAlaArglieLeuTrplieAspThrGlyAlaGln100105110ProProGluAlaAlaHisGluAspProValProAspAlaValAlaGlu115120125AsnAlaArgValAlaValLeuLeulieAspAspLeuAlaGlnAspGly130135140ThrValGlyGluAlaAspGluProHisArgTyrLeuThrArgLeuLeu145150155160TrpAlaPheAlaLeuLeuArgAsnLeuLeuAlaArgArglieGluArg165170175CysHisLeuLeuHisLeuThrProLeuArgHisAlaLeuProlieAla180185190AlaAlaTrpHisGlyLeuAlaLysSerValValLeuGluSerProArg195200205PheAlaSerGlyGlyValTrpLeuSerAspSerAspAlaAlaArgVal210215220ThrProGluLeuValAlaArgGluLeuSerGluLeuMetProAlaArg225230235240HisArgAsplieAlaTrplieAspGlyArgArgCysGluArgValLeu245250255ValAlaHisAlaHisGlyAlaValAlaProThrValGlyProLeuAla260265270CysGlyGluThrValLeulieThrGlyGlyAlaGlyGlyLeuGlyLeu275280285AlalieAlaGluArgLeuThrThrAspPheAlaLeuArgValValLeu290295300CysGlyArgArgAlaAlaHisAlaLeuAspAspArgGlnArgAspTrp305310315320LeuAlaArgHisProThrValArgTyrValSerAlaAspValSerAsp325330335ProAspGlnValArgThrLeuMetAlaGluLeuGlyAlaAlaThrPro340345350LeuAlaAlaValPheHisAlaAlaGlyGlySerAspAspAlaProLeu[4667]355360365[4668]ArgAlaLysSerLeuArgAlalieArgAlaValLeuAlaProLysVal370375380LeuGlyThrGlnTrpLeuAspArgHisAlalieGlyLeuGlyValArg385390395400TyrPheValCysPheSerSerValAlaSerLeuAlaGlySerValGly405410415GlnAlaAspTyrAlaSerGlyAsnGlyPheMetAspGluTyrCysArg420425430LeuArgGlnAlaAlaSerAspAlaSerGlyGlyCysCysTyrLeuSer435440445lieAsnTrpProTyrTrpArgGluGlyGlyMetArgAlaAspProGly450455460ArgLeuGluArglieAlaAlaAspSerGlyLeuThrSerLeuGlyThr465470475480ValGluGlyValAspSerLeuLeuAlaLeuLeuAlaArgProAlaPro485490495GlnValAlaValLeuProGlyGluArgAlaArglieAspArgAlaPhe500505510LeuAlaThrGlnProProGluArgProValAlaArgAlaGluAlaAsp515520525AspProAlaSerGlyLeuArgGluLeuValAlaArgThrLeuLysMet530535540AlaProGluAlalieAspAlaAspThrProPheSerGluTyrGlyPhe545550555560AspSerlieLeuLeuThrGluLeuAlaSerThrlieGlyArgArgTyr565570575ProGlyLeuGluLeuGluValGlyValPheLeuAspHisProThrLeu580585590ArgGluLeuAsnAspTrpLeuArgValArgLeuAlaAspThrSerSer595600605AspProAsplieAlaArgAlaAlaArgProAspGluValAlaProSer610615620GlnGlyAspProLeuLeuAspArgLeuArgAlaLeuAlaSerThrlie625630635640LeuLysLeuProAlaThrAsplieAspAlaGluThrSerTrpSerThr645650655PheGlyPheAspSerlieSerLeuAsnGluPheAlaValLeulieAla660665670[4706]ArgAspPheArgThrGlyProLeuAlaAlaAspLeuPheLeuSerCys675680685AlaThrLeuAlaGluLeuAlaAlaHisLeuAlaAlaArgValArgAla690695700ThrGluProAlaHisGluArgAlaProAlaArgArgValAlaValSer705710715720AlaLeuThrAsnProGlyProValProAspAlaThrGluGlulieAla725730735lielieGlyMetAlaGlyArgPheProGlyAlaSerAspLeuProAsp740745750PheTrpArgAsnLeuValGluAspArgHisAlaValThrGluliePro755760765AlaAspArgTrpArgTrpGlnAspTyrAlaAlaGlyAlaProGlyLys770775780ThrAspCysHisTyrAlaAlaPheLeuThrAlalieAspArgPheAsp785790795800AlaAlaHisPheSerlieSerProArgGluAlaGluLeuMetAspPro805810815GlnGlnArgLeuLeuLeuGluCysAlaTrpGluAlaPheGluAsnAla820825830AlalieAlaProAspSerValArgGlyArgArglieGlyLeuPhePhe835840845GlyAlaGluLysHisAspTyrLeuSerLeulieAlaAspAlaAspVal850855860AsplieAspProTyrlieAsnThrGlyAsnAlaHisAlaMetLeuThr865870875880AsnArgValSerTyrPhePheGlyLeuThrGlyProSerMetThrLeu885890895AsnThrAlaCysSerSerSerMetThrAlalieHisAlaAlaValAla900905910SerLeuArgSerGlyAlaSerGluMetAlaLeuAlaGlyGlyValAsn915920925lieLeuLeuSerProGlyLeuPheValLeuAsnArgLysMetGlyMet930935940LeuThrAlaCysAspArglieLysProPheAspArgAspAlaSerGly945950955960HisLeuPheGlyGluGlyLeuGlyLeuValLeuLeuLysProLeuArg965970975LeuAlaLeuArgAspGlyAspProValHisGlyVallieArgAlaVal[4745]980985990AspValThrHisGlyGlyHisGlyArgPheLeuThrAlaProHisAla99510001005ProSerHisGluAlaLeulieArgGlyValLeuHisGlnAlaGlyAla101010151020SerProAlaAspValAspTyrLeuGluAlaGlnGlySerGlyAspAla1025103010351040SerThrAspArgMetGluLeuGluThrTyrHisAlaLeuTyrGlyAsp104510501055ArgAlaGlyGlyGluProLeuProlieGlySerValLysGlyHislie106010651070AlaHisLeuGlyAlaAlaSerGlyValThrAlaLeulieLysValLeu107510801085LeuCysMetSerHisAspArgLeuLeuProlieLeuHisHisArgGln109010951100lieAsnTrpProHisAspGluProPheAlaGlyArgLeuLeuThrAla1105111011151120HisAlaGluTrpArgProLysTrpValAspGlyCysArgValProArg112511301135ValAlaAlaValHisAsnPheGlyTyrGlyGlyValAsnGlyHisLeu114011451150LeuValArgGluTyrLeuArgAlaProAlaValGluAlaArgTrpPro115511601165AlaValAlaArgLeuTrpValValSerAlaArgThrAlaAlaGlnLeu117011751180ArgGlnSerValAlaArgLeuValAlaTyrLeuArgAspGlyGlyTyr1185119011951200ArgLeuHisGlyGlnArgGluProGlyValGluSerlieAlaPheThr120512101215LeuGlnThrGlyArgGlnProLeuAsnHisArgLeuAlaLeuTrplie122012251230GlyAspThrPheLysValAspGlyLeuArgThrAlaLeuAlaArgLeu123512401245AspAlaTrpLeuAlaAspGlyHisGlyAspAlaHisLeuLeuThrGly125012551260ValValThrProAlaSerArgValGluProSerProAlaSerAspGlu1265127012751280AlaAlaAspAspProLeuArglieAlaAlaArgTrplieSerGlyAla[4783]128512901295[4784]ProlieAsnTrpThrAlaCysTyrGlyAspThrArgProAlaArglie130013051310SerLeuProValTyrProPheAlaAlaThrArgTyrTrpValAlaSer131513201325ArgLysAspGlyAspGlyAlaAlaProGlnGlyGlySerThrAlaPro133013351340GlyAlaAlaProAlaLeuAlaGlyValProSerAlaAlaArgAlaArg1345135013551360ProAlaAlaValLeuHisProLeuLeuHisArgLeulieProAlaAsp136513701375ArgAlaLeuArgPheLeuSerThrPheSerGlyAspGluProPhePhe138013851390ArgAspHisArgValArgSerAlaAlaMetLeuProGlyAlaAlaTyr139514001405CysGluMetPhelieAlaAlaAlaThrLeuAlaMetProGluSerGlu141014151420ArgCysSerAlaGlyPheArgLeuGluHisLeuValTrpThrArgPro1425143014351440LeuValProSerAspGlyThrAlaAsplieAlaValThrMetGluPro144514501455AlaMetThrProAlaArgArgAlaGlylieAspGlyArgAspValPhe146014651470ArgLeuThrValGlyAspValArgSerAsnAspAlaGlnProTyrAla147514801485GlnAlalieValValProGluGlnArgAspThrMetProValProPro149014951500ValValAlaValGluGlnLeuAlaValTyrSerArgGlyThrArglie1505151015151520AspGlyAlaArgLeuTyrArgArgPheAlaGluLeuGlyPheGluTyr152515301535GlyProSerHisArgCyslieAspAlaLeuPheTyrAspGlyAspGlu154015451550ValLeuAlaArgMetArgProTyrGluProAspThrHisGlyThrGly155515601565TyrLeuLeuAlaProGlyLeuLeuAspSerAlaLeuGlnAlaSerlie157015751580GlyPheAspProAlaAlaLeuAspAlaAsnHisAspGlyTyrLeuAsp1585159015951600GlyArgLeuSerProLeuLeuProPheAlaLeuGluArgLeuArgCys[4823]160516101615HisArgThrSerGlyLeuSerGluHisValArgTrpValArgLeuArg162016251630ArgArgAlaGlyMetAlaGlyAsnProValValLysLeuAspValAsp163516401645LeuLeuAspAlaAspGlyHisValCysAlaArgLeuGluGlyTyrSer165016551660[4830]AlaArgArgValLysSerGluValAlaAlaCysAlaValProAlaThr1665167016751680ArgArgAspAlaAlaGlyThrlieProValProLeuProGluProAla168516901695ValTrpArgAspGluAspGlyValArgValTyrSerCysArgPheGlu170017051710ProAsnAspLeuTyrLeuArgHisHisArglieAspGlyGlnAlaLeu171517201725LeuProAlaValAlaLeuLeuAspLeuAlaArgVallieAlaAspThr173017351740LeuProArgAspGluAlaAlaAlaAlaLeuArglieSerArgAlaThr1745175017551760TrpGlnLysProValPheValGlyThrProGlyArgAspAlaAlaLeu176517701775ArgValAlaHisArgAspGlyCysTrpThrAlaThrLeuGluSerGly178017851790AsnGlyMetGlnGlyGlyValAlaProTyrAlaGlnCysAspLeuThr179518001805TrpThrAlaAspValProAspAlaAspProLeuProAspArgAlaArg181018151820ArgAlaGlyAlaProLeuSerLysAlaAspValAlaGlnArgPheAla1825183018351840GlySerLeuValArgGluSerGlyAsnValLeuGlyLeuProGlyGly184518501855lieArglielieGlyCysGluSerSerAlaAspThrThrTrpLeuThr186018651870LeuAlaCysGluAspGlyAsnAspAspSerAlaGlyGlyAlaArgSer187518801885ThrAlaPheGlyLeuLeuPheAlalieValAspLeuCysGlyArgAsp189018951900ArgGluAlaAlaGlyLeuAlalieProTyrGlylieAspGluCysLeu1905191019151920[4862]LeuTyrArgAlaPheProArgGlnValArgValGlulieArgAlaAla192519301935SerProGlyThrSerAlaArgThrArgValTyrAsplieArglieAla194019451950AspLeuGluHisArgProValMetGlnlieThrArgLeuThrMetVal195519601965AlaProArgGluAlaAlaAlaGlyProAlaAlaSerProThrAlaLeu197019751980ThrAspAspAlaThrLeuAlaLeuGlnGlnCysLeuArgGluTrpVal1985199019952000GlyGluLeuGlnLyslieAspProAspGlulieGlyLeuAspAspAsp200520102015LeuAlaThrPheGlyLeuAspSerPheSerPheThrAlaLeuSerSer202020252030ArgLeuAsnAspArgPheAlalieAspThrMetProThrValPhePhe203520402045GluHisGlyThrValArgAlaLeuAlaAsnGlyLeuLeuArgThrTyr205020552060ProAsnLeuAlaAlaGlyLeuArgAspAspGlyArgValThrArgArg2065207020752080AspGluProGlyProGlyAlalieSerProValSerAlaSerLeuPro208520902095ArgGlyProAlaGlyThrAspAlalieAlalieValGlyMetAlalie210021052110ArgLeuProArgAlaLysThrLeuAspAlaPheArgArgArgLeulie211521202125ThrProHisGluGluAlaSerGlyLeuGluAlaAlaAlaSerAsnArg213021352140AlaAspThrAspLysAsnGlyAlaAspAlaAlaThrGlnAlaAspArg2145215021552160ValProProAlaTrpPheGlyAlaAspValGluGluGlyAlaSerGly216521702175ArgGlyLeuValSerValLeuGluThrValTrpHisLeuPheGluAsp218021852190AlaGlyMetAlaProAlaThrLeuArgGlySerAspThrGluValTyr219522002205LeuGlyValSerSerArgAlaThrAlaGluSerAspLeuProValAla221022152220LeuProAlaSerHisAlaPheSerAsnArglieSerPheGluPheAsp[4901]2225223022352240PheHisGlyProSerGlnAlalieAspThrGlyCysSerSerSerLeu224522502255AlaAlaLeuAlaArgGlyAlaGluArgLeuArgGlnArgArgCysGly226022652270LeuValValValGlyGlyValAsnVallieAlaProThrCysGlyGly227522802285GluAlaArgAlaSerAspAsnMetAspGlyGlylieGlyMetValLeu229022952300LeuLysArgLeuAspAspAlaLeuArgAspGlyAspArglieHisAla2305231023152320ValValAlaAlaValAlaGluAsnHisGlyGlyArgThrLeuSerLeu232523302335AlaAlaProSerValGluAlaGlnArgGluLeuLeulieAlaAlaTyr234023452350ArgGlnAlaGlyValGlyAlaAlaGlylieGlyHislieGluLeuHis235523602365GlyAlaGlyThrAlaValAlaAspThrlieGluCysGluAlaLeuArg237023752380AspAlaPheThrGluLeuArgGlyArgGlnAlaValProArgGluAla2385239023952400ProArgCysMetLeuGlyAlalieGlyGlyArgLeuGlyAlaLeuGly240524102415AlaAlaGlyGlyMetAlaGlyVallieLysThrAlaLeuMetLeuArg242024252430HisGlnThrlieAlaGlyHisProProTrpLeuAspThrArgLeuVal243524402445AspLeuGluGlySerGlyLeuGluAlaValGlyGluAlaAlaArgPro245024552460TrpProAlaThrHisAsnAlaGlnProArgCysAlaGlyValSerSer2465247024752480PheGlyAlaThrGlyAlaAsnAlaHisAlaValLeuLeuGluHisVal248524902495AlaValProAlaHisArgProAspGlyMetSerProProAspSerAla250025052510ArgLeuPheLeuLeuSerAlaHisSerGluThrAlaLeuArgAlaGln251525202525AlaArgAlaLeuAlaSerHisLeuAlaArgCysAlaSerAlaAspAsp[4939]253025352540[4940]lieMetAlaAspAlaArgGlyGlnValLeuHisAspMetAlaTyrThr2545255025552560LeuGlnAlaGlyArgAspAlaLeuProTrpArgPheGlyValValAla256525702575ThrThrSerAspAlaLeuGlnArgGlnLeuArgLeuPheSerAspAsp[4945]258025852590ProThrGluGlyCysAlaAlaLeuAlaGluPheArgValAlaHisVal259526002605AspProLeuArgProAspPheSerAspAspGluAspMetGlnAlaLeu261026152620ValArgGlnTrpLeuGluLysGlyLysArgMetHisValLeuArgLeu2625263026352640TrpLeuAspGlyValSerlieAspTrpArgAlaAlaTyrArgThrArg264526502655ProGlyAlaLeuThrAspLeuProProTyrProPheProSerAlaVal266026652670AlaLeuHisGluAlaGlyThrAspCysAlaValAlaAlaAlaGlnThr267526802685ProAlaArgAlaAlaGlyAspLeuAlaValAspValArglieArgArg269026952700LeuLeulieGlyGlyProGluArgHisAlaSerlieAspGlyAsnSer2705271027152720ProAlaAspAlaGluAlaLeuMetGlnArglieArgHisValGlyGlu272527302735LeuLeuGlyAlaThrLeuSerValGlyAspAlaTyrArgTyrProAsn274027452750ValAspAlaLeuArgThrAlaLeuArgAspArgGluArgArgAlaGlu275527602765AlaAlaAspGlyValAlaAlaAlaAlaSerGlyAspProPheAlaArg277027752780TyrTyrArgMetAlaPheGlyGlulieAspGlyPheGluArgAlaLeu2785279027952800LeuGluLeuAspAspAspVallieLeuAspValLeuHisAsnValSer280528102815ProGlyLysProProLeuLeuLeuLeuAlaProMetGlyCysLeuGly282028252830ThrAlaTrpLeuHisGlnValArgGluPheAlaAlaAspTyrSerlie283528402845lieValCysHisTyrProGlyHisAlaAlaSerSerGlyThrAlaAla[4979]285028552860LeuLeuSerAlaAlaGlyThrLeuAlaAspValAlaArgLeulieTrp2865287028752880ThrAlaLeuAspArgLeuGlylieGlyLeuProValHisLeuValGly288528902895TrpSerMetGlyGlyLeuValAlaGluCysMetThrAlaValAspPro290029052910GluArgValAlaSerMetThrLeuValAsnGlyLeuAspAlaProGly291529202925AsnGlyGlyLeuAspArgAlaMetArgGluLeuValHisGluValSer293029352940ThrGlnValAlaProGluValTyrArgHisPheGluGlyAspHisPhe2945295029552960AlalieLysAlaCysTyrAspAlaSerlieLeuAspAlaTyrLeuArg296529702975CysAlaSerAlaThrThrValLysLysAspLeuAlaAlaProArgPhe298029852990ProValLeuValLeuAlaGlyGlyArgAspArgVallieProProGlu299530003005AlaSerArgArgLeuTrpGluArgPheAspGlyAlaAlaPheGluLeu301030153020LeuAspGluAlaGlyHisTyrLeuProMetThrHisAlaAspTrpPhe3025303030353040AsnGluArgLeuArgGlnHisLeuGluValThrVal30453050<210>21<211>145<212>PRT<213>Psuedomonassp.NO.2663<400>1MetSerAspLeuThrGlySerTrpSerLeuGluGluSerGluPheArg151015LeuAlaAsnGlyAsnlieThrTyrProLeuGlyArgAsnProSerGly202530ArglielieTyrMetGluSerGlyHisMetSerValLeuLeulieAla354045LysGlyArgAlaAsnValGlyPhelieProGluLysPheTrpLeuAsn505560liePheGlylieLysArglielieGlyLeulieArgLeulieArgAla[5018]65707580AsnSerGlyValLeuGlyTyrSerGlyArgTyrSerThrAspGlyLys[5020]859095ThrValLeuHisHisValAspLeuSerSerTyrProAspPheValGly100105110ThrArgLeuValArgGluValArgTyrGluGluGlyArgLeuValLeu115120125GluAsnSerThrAspSerGlySerSerArgLeuValTrpAlaArgGlu130135140lie14權(quán)利要求一種抗腫瘤活性的天然產(chǎn)物FR901464的生物合成基因簇，該基因簇核苷酸序列如SEQIDNO.1所示，其特征在于包含編碼FR901464生物合成所涉及的20個(gè)基因，具體為1)5個(gè)I型聚酮合成酶基因fr9E，fr9F，fr9G，fr9H，fr9I；2)雜合的聚酮/聚肽和非核糖體聚肽合成酶基因fr9C，fr9D；3)3個(gè)獨(dú)立的?；D(zhuǎn)運(yùn)酶基因fr9B，fr9J，fr9O；4)4個(gè)與聚酮骨架烷基化相關(guān)基因fr9K，fr9L，fr9M，fr9N；5)4個(gè)后修飾基因fr9P，fr9Q，fr9R，fr9S；6)2個(gè)轉(zhuǎn)錄調(diào)控相關(guān)基因fr9A，fr9T。2.如權(quán)利要求1所述的FR901464的生物合成基因簇，其特征在于編碼的I型聚酮合成酶包含下述模塊或結(jié)構(gòu)域酰基載體蛋白ACP，酮基合成酶結(jié)構(gòu)域KS，酮基還原酶結(jié)構(gòu)域KR,脫水酶結(jié)構(gòu)域DH，甲基轉(zhuǎn)移酶結(jié)構(gòu)域MT，烯醇還原酶ER，氧化酶結(jié)構(gòu)域0X，烯酰輔酶A水合酶結(jié)構(gòu)域ECH，硫酯酶結(jié)構(gòu)域TE;5個(gè)聚酮合成酶基因編碼的聚酮合成酶分別具有如下的結(jié)構(gòu)域組成Fr9E,DH-KR-MT；Fr9F,ACP-ER-KS-DH-KR-ACP-KS-DH-KR-MT-ACP-KS；Fr9G,DH-KR-ACP-KS-KR-ACP；Fr9H,KS-ECH-ACP-ACP-ACP-KS-OXFr9I,KR-ACP-KS-DH-ACP-KS-TE。3.如權(quán)利要求1所述的FR901464的生物合成基因簇，其特征在于編碼的聚酮合成酶及非核糖體聚肽合成酶包含下述模塊或結(jié)構(gòu)域非典型的脫水酶DH*，非典型的酮基還原酶KR*,甘油酰基轉(zhuǎn)移酶結(jié)構(gòu)域Gf，肽?？s合酶結(jié)構(gòu)域C，腺苷化酶結(jié)構(gòu)域A，肽?；d體蛋白PCP；雜合的聚酮/聚肽和非核糖體聚肽合成酶基因編碼的酶分別具有如下的結(jié)構(gòu)域組成Fr9C,DH*-KR*-GT*-ACP-KS-KR-ACP-KS-ACP-ACP-TE-KS-ACP-C；Fr9D,C-A-PCP-KS。4.如權(quán)利要求1所述的FR901464的生物合成基因簇，其特征在于編碼的3個(gè)獨(dú)立的?；D(zhuǎn)運(yùn)酶Fr9B，F(xiàn)r9J，F(xiàn)r90均識(shí)別丙二酰輔酶A底物。5.如權(quán)利要求1所述的FR901464的生物合成基因簇，其特征在于4個(gè)與聚酮骨架烷基化相關(guān)基因編碼的蛋白分別為Fr9K,3-羥基-3-甲基戊二酰輔酶A合成酶；Fr9L，乙烯基輔酶A水合酶；Fr9M，?；d體蛋白；Fr9N，編碼酮基合成酶。6.如權(quán)利要求1所述的FR901464的生物合成基因簇，其特征在于4個(gè)后修飾基因編碼的蛋白分別為Fr9P，非血紅素鐵依賴的氧化酶；Fr9Q，環(huán)合酶；Fr9R，細(xì)胞色素P450羥化酶；Fr9S，乙酰基轉(zhuǎn)移酶。7.如權(quán)利要求1所述的FR901464的生物合成基因簇，其特征在于2個(gè)轉(zhuǎn)錄調(diào)控相關(guān)基因編碼的蛋白分別為Fr9A，轉(zhuǎn)錄調(diào)控因子；Fr9T，雙鳥(niǎo)苷酸環(huán)化酶。8.一種根據(jù)權(quán)利要求1所述的FR901464的生物合成基因簇在制備催化合成抗腫瘤活性的天然產(chǎn)物FR901464的蛋白中的應(yīng)用。9.根據(jù)權(quán)利要求2、3、4所述的FR901464的生物合成基因簇在制備催化合成聚酮/聚肽鏈的蛋白中的應(yīng)用。10.根據(jù)權(quán)利要求5、6、7所述的FR901464的生物合成基因簇在制備催化聚酮/聚肽烷基化、后修飾及調(diào)控的蛋白中的應(yīng)用。全文摘要本發(fā)明是一種由假單胞菌產(chǎn)生的具有抗腫瘤活性的天然產(chǎn)物-FR901464的生物合成基因簇的克隆、測(cè)序、分析、功能研究及其應(yīng)用。整個(gè)基因簇共包含20個(gè)基因5個(gè)聚酮合成酶基因，1個(gè)雜合的聚酮/非核糖體聚肽合成酶基因，1個(gè)非核糖體聚肽合成酶基因，3個(gè)獨(dú)立的酰基轉(zhuǎn)移酶基因，4個(gè)與聚酮骨架烷基化相關(guān)基因，4個(gè)后修飾基因，2個(gè)調(diào)控相關(guān)基因。通過(guò)對(duì)上述生物合成基因的遺傳操作可阻斷FR901464的生物合成。本發(fā)明所提供的基因及其蛋白可用于該化合物的基因工程、蛋白表達(dá)、酶催化反應(yīng)等，也可以用來(lái)尋找和發(fā)現(xiàn)可用于醫(yī)藥、工業(yè)或農(nóng)業(yè)的化合物或基因、蛋白。文檔編號(hào)C07K14/21GK101818158SQ20101013604公開(kāi)日2010年9月1日申請(qǐng)日期2010年3月30日優(yōu)先權(quán)日2010年3月30日發(fā)明者唐功利,張鳳申請(qǐng)人:中國(guó)科學(xué)院上海有機(jī)化學(xué)研究所