專利名稱:一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的制作方法
技術(shù)領(lǐng)域:
本發(fā)明涉及生物技術(shù)領(lǐng)域,尤其涉及一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽。
背景技術(shù):
肝臟是人體內(nèi)最大的消化腺。也是體內(nèi)新陳代謝的中心站。據(jù)估計(jì),在肝臟中發(fā)生的化學(xué)反應(yīng)有500種以上,實(shí)驗(yàn)證明,動(dòng)物在完全摘除肝臟后即使給予相應(yīng)的治療,最多也只能生存50多個(gè)小時(shí)。這說明肝臟是維持生命活動(dòng)的一個(gè)必不可少的重要器官。肝臟的血流量極為豐富,約占心輸出量的1/4。每分鐘進(jìn)入肝臟的血流量為1000-1200ml。肝臟的主要功能是進(jìn)行糖的分解、貯存糖原;參與蛋白質(zhì)、脂肪、維生素、激素的代謝;解毒;分泌膽汁;吞噬、防御機(jī)能;制造凝血因子;調(diào)節(jié)血容量及水電解質(zhì)平衡;產(chǎn)生熱量等。在胚胎時(shí)期肝臟還有造血功能。
肝臟疫病分為肝炎、肝硬化、脂肪肝、肝癌等。現(xiàn)代醫(yī)學(xué)實(shí)驗(yàn)證明,肝病病毒侵入人體后,并不直接引起肝細(xì)胞的損害,只是在肝細(xì)胞內(nèi)吸收營養(yǎng)賴以生存,并在肝細(xì)胞內(nèi)復(fù)制、繁殖。其復(fù)制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細(xì)胞膜上,引起人體免疫系統(tǒng)對(duì)這些抗原物質(zhì)產(chǎn)生免疫反應(yīng),這種反應(yīng)造成肝細(xì)胞的損傷、壞死。免疫反應(yīng)的強(qiáng)弱決定于肝臟受損程度及臨床癥狀輕重。這場(chǎng)由病毒引發(fā)的、免疫系統(tǒng)對(duì)肝細(xì)胞的戰(zhàn)爭,使大約25%的患者的肝臟成為戰(zhàn)火連綿的戰(zhàn)場(chǎng),肝臟的損傷由此加重。肝病的危害絕不僅僅限于肝臟本身,它還可以引起其它多種疾病。常見的有(1)糖尿??;(2)胰腺炎;(3)膽道感染;(4)功能性腎衰竭;(5)膽汗性腎??;(6)腎小球腎炎;(7)腎小管酸中毒;(8)溶血性貧血;(9)再生障礙性貧血;(10)心肌炎和心包炎;(11)結(jié)節(jié)性動(dòng)脈炎;(12)消化性潰瘍;(13)自發(fā)性腹膜炎;(14)性激素代謝紊亂;(15)甲狀腺功能改變;(16)肝性骨病,等等。肝病不僅對(duì)患者的身體甚至生命造成危害,而且對(duì)患者心理上的打擊也是十分沉重的。無論是肝病患者還是病毒攜帶者,在生活、社交、求職、升學(xué)等方面都會(huì)受到嚴(yán)重影響。
生物基因組中可轉(zhuǎn)錄表達(dá)的序列(即基因)僅占總序列的3-5%,對(duì)這部分序列進(jìn)行測(cè)定,將直接導(dǎo)致新基因的發(fā)現(xiàn),并獲取基因組中與產(chǎn)業(yè)化關(guān)系最為密切的信息。20世紀(jì)80年代,高通量的自動(dòng)測(cè)序的出現(xiàn),使從質(zhì)?;パa(bǔ)脫氧核糖核酸(Complementary DNA,簡稱cDNA)文庫隨機(jī)選取許多cDNA克隆和決定來自非載體兩端的幾百個(gè)堿基的DNA序列成為可能。這些短的DNA序列叫做“表達(dá)序列標(biāo)簽”(Expressed Sequence Tags,簡稱ESTs)。表達(dá)序列標(biāo)簽的概念最早是由Adams等在1992年提出來的(Nature,355,642-644)。1992年Sikela和Matsubara(Sikela,et al.Nucleic AcidsRes.19,1837-1843;Matsubara,et al.Nature Genetics,2,173-179)針對(duì)獲得大量信使核糖核酸(mRNA)序列的迫切需要,提出大規(guī)?;パa(bǔ)脫氧核糖核酸(cDNA)測(cè)序的研究戰(zhàn)略。隨后Venter創(chuàng)立了大規(guī)模表達(dá)序列標(biāo)簽技術(shù)。其基本特征就是從以質(zhì)粒為載體,構(gòu)建完成的目的組織互補(bǔ)脫氧核糖核酸(Complementary DNA,簡稱cDNA)文庫中,隨機(jī)選擇許多cDNA克隆,利用質(zhì)粒上攜帶的通用引物對(duì)cDNA兩端進(jìn)行一輪脫氧核糖核酸序列測(cè)定,所獲得的來自3’端或5’端的幾百個(gè)堿基的非載體短脫氧核糖核酸(DNA)序列。簡而言之,表達(dá)序列標(biāo)簽是來自表達(dá)基因片段3’端或5’端的短脫氧核糖核酸序列,代表一個(gè)表達(dá)基因的部分轉(zhuǎn)錄片段。
表達(dá)序列標(biāo)簽可用于新基因克隆、人類基因組圖譜繪制、基因組序列編碼區(qū)的確定等。如果一個(gè)表達(dá)序列標(biāo)簽在基因組中只出現(xiàn)一次,那么它可以作為序列標(biāo)簽位點(diǎn)(STS)。由表達(dá)序列標(biāo)簽構(gòu)建的物理圖譜叫表達(dá)圖或轉(zhuǎn)錄圖(expression or transcript map)。利用表達(dá)序列標(biāo)簽進(jìn)行基因圖制作,可以加快序列標(biāo)簽位點(diǎn)的制作和新基因的染色體定位。表達(dá)序列標(biāo)簽可以作為基因特異性探針,對(duì)組織特異性基因表達(dá)的研究具有重要的作用。表達(dá)序列標(biāo)簽還可以進(jìn)行新基因的遺傳進(jìn)化關(guān)系分析。表達(dá)序列標(biāo)簽可以對(duì)所有動(dòng)植物的基因作為一種數(shù)據(jù)庫,通過不同的序列比較可以獲得保守序列片段,從而獲得基因的遺傳進(jìn)化圖譜。正因?yàn)楸磉_(dá)序列標(biāo)簽具有如此的優(yōu)越性,因此表達(dá)序列標(biāo)簽測(cè)序已經(jīng)成為許多基因組研究機(jī)構(gòu)的工作重點(diǎn)。
由于本發(fā)明人類肝臟特異表達(dá)基因與一些肝臟疾病相關(guān),因此,研究人類肝臟中特異表達(dá)的表達(dá)序列標(biāo)簽對(duì)探索肝臟疾病的發(fā)病機(jī)理及研制肝病的治療藥物具有重要意義。
發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問題是提供一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽。
本發(fā)明要解決的技術(shù)問題通過如下技術(shù)方案實(shí)現(xiàn)本發(fā)明提供了一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的序列,其包括(a)SEQ ID No.1~SEQ ID No.50所示的序列;(b)SEQ ID No.1~SEQ ID No.50所示的序列中每條序列的互補(bǔ)序列;(c)與SEQ ID No.1~SEQID No.50所示的序列中每條序列有至少70%同源性的序列,及(d)上述(a)~(c)中一條或數(shù)條的組合。
較佳地,所述序列包括具有SEQ ID No.1~SEQ ID No.50所示的序列。
本發(fā)明還提供了一種探針分子,所述的探針分子含有上述序列中約8-100個(gè)連續(xù)的核苷酸。
由本發(fā)明的在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽,可以方便的尋找出在人類肝臟中特異表達(dá)的表達(dá)序列標(biāo)簽,從而尋找出人類肝臟疾病相關(guān)基因,從而在研究肝臟疾病的致病機(jī)理以及開發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
具體實(shí)施例方式
下面結(jié)合附圖
對(duì)本發(fā)明作進(jìn)一步詳細(xì)的說明。應(yīng)理解,這些實(shí)施例僅用于說明本發(fā)明而不是限制本發(fā)明的范圍。下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法,通常按照常規(guī)條件如Sambrook等人,分子克隆實(shí)驗(yàn)室手冊(cè)(New YorkCold Spring Harbor Laboratory Press,1989)中所述的條件,或按照制造廠商所建議的條件。
實(shí)施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來源于5個(gè)成年男性,在肝臟切除手術(shù)后,將肝臟組織立即置于液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織,用研缽研碎,加入盛有裂解液的50ml管,充分振蕩后,再移入玻璃勻漿器內(nèi),勻漿后移至50ml新管,抽提總RNA(TRIzol Reagents,Gibco,NY,USA)。用甲醛變性膠電泳鑒定總RNA質(zhì)量。用帶Oligo d(T)的纖維素柱分離總RNA中的mRNA,定量。
實(shí)施例2cDNA文庫的構(gòu)建(Constuct ion of cDNA library)以mRNA為模板,合成雙鏈cDNA。補(bǔ)平末端后,加含EcoRI切點(diǎn)的接頭。磷酸化EcoRI末端后,用XhoI限制性內(nèi)切酶消化1.5小時(shí),再進(jìn)行片斷分離。過柱篩選長度>500bp的片段,用酚-氯仿抽提,乙醇沉淀,無菌水溶解,連接至Uni-ZAP XR載體(Strategene,CA9203,USA),以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene,CA9203,USA)進(jìn)行包裝,宿主菌使用XL 1 Blue MRF’(Strategene,CA9203,USA)細(xì)菌。涂板并測(cè)定滴度。
實(shí)施例3測(cè)序及數(shù)據(jù)庫建立(Seqencing and Database Constructing)挑選文庫中有外源片段插入的克隆,擴(kuò)增后抽提質(zhì)粒(QiagenGermany),用T3和T7作為3’和5’端的通用引物,采用終止物熒光標(biāo)記(Big-Dye,Perkin-Elmer,USA)的方法,在ABI 377測(cè)序儀(Perkin-Elmer,USA)上進(jìn)行EST大規(guī)模測(cè)序。測(cè)序結(jié)果用FACTURA軟件去除載體序列,傳輸?shù)絊UN Ultra 450 Server上進(jìn)行下一步的處理。所有的序列信息再用GCG軟件包(Wisconsin group,USA)中的BLAST和FASTA軟件搜索已有的數(shù)據(jù)庫(Genebank+EMBL),將無同源性或同源性低于95%的序列視為新基因建立數(shù)據(jù)庫。
實(shí)施例4基因的全長克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎(chǔ)上,進(jìn)行cDNA全長克隆,分兩階段進(jìn)行(1)“電子克隆”(Electronic Cloning)以新基因片段序列作為探針?biāo)褜bEST數(shù)據(jù)庫,將重疊序列>50bp,同源性在98%以上的表達(dá)序列標(biāo)簽(Expressed Sequence Tag,簡稱“EST”)序列認(rèn)為同一序列(Consensus Sequence),取出并用AUTOASSEMBLER軟件進(jìn)行連接,部分EST可以延伸探針序列。再用STRIDER軟件分析被延伸的序列是否具有完整的開放閱讀框架(Open ReadingFrame,ORF),用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和氨基酸水平上是否與其他物種有同源性,以幫助判別所得到的基因全長完整性如何。通過電子克隆的方法,通??色@取人肝臟相關(guān)基因的全長序列。
(2)cDNA末端快速擴(kuò)增(Rapid Amplification of cDNAEnds,RACE)如果通過“電子克隆”方法仍未得到完整的cDNA全長,則在已有序列5’或3’端設(shè)計(jì)引物,在人類肝臟Marathon-Ready cDNA文庫(Clontech Lab,Inc,USA)中進(jìn)行長距離PCR反應(yīng)。然后對(duì)PCR產(chǎn)物克隆、測(cè)序。用AUTOASSEMBLER及STRIDER軟件分析被延長的序列有無完整的ORF,如無,重復(fù)上述過程直至獲得全長。
(3)RT-PCR對(duì)于5’和3’端的已知的序列,如果中間有一段間隙(gap)無法從已有的公共數(shù)據(jù)庫或自身數(shù)據(jù)庫獲得,可考慮采用RT-PCR的方法。在序列5’端設(shè)計(jì)引物,3’端引物采用Oligo-dT,在肝臟總RNA庫中進(jìn)行擴(kuò)增。然后對(duì)產(chǎn)物進(jìn)行克隆、測(cè)序。最后拼接便獲得全長。
通過組合使用上述3種方法,可獲得人肝臟相關(guān)蛋白的全長編碼序列。
序列表<110>上海人類基因組研究中心<120>在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽<130>NP-10157<160>50<210>1<211>467<212>DNA<213>Homo sapiens<400>11 gctctatttt caggaaaatt tctatcatat aaaagcttat ttcagttatt ataaaagtgc61 ttattctctg gttatttctt ctttttatat aatcttgtca tgtttcatag tagcagtatc121 ttctgctatc tctctgagta tattaattat actttatttc tacttcagct actgttttct181 acaggacata ctgcctctaa ttcttaaaca ggttctggag tatgaactgt cttgtttctc241 attgacacaa cactctccag tcacatgtat tcggctttcc catttctgcc agttcctcct301 ttacatcttc caaaagttgt gtctctttaa tatgttgctt cctcttcctt atgcctgtgg361 atttatatat tttttagtct tttgctcaaa atttttagtc ccttactagg aaggaacatg421 gaaaaacatg tgttcaatca cgaagtttaa ctggaagtct gctgtta<210>2<211>461<212>DNA<213>Homo sapiens<400>21 aaaaaataaa aatcatttat ttaaacaaaa atatacacac agtgtacaat gaatatggtt61 tacacagaac aataaaacaa aaatgatatc tcttaaatgc caacataaaa tgatctattt121 atgcctagaa aaaatttcta ggaatacaca ttattgattc atctaaaata catatataac181 ataaaaagaa aacaaatgcc aacctttcag atgagtacca caaaaaccta aaagttaaat241 gttagattaa ataaaattat aaaatatcca cacgagaagt atgtgtatac aaagtattgt301 atgtatgcag tgaaatgaga gtgcccatac agttatatgc aaacttttga aagtttcata
361 gttgtttaac aaaatatttt taatgttgat agtgacataa aaacatcaca agaattatct421 agataatata gcaatattgc caaaatttga gtcaatgtta c<210>3<211>543<212>DNA<213>Homo sapiens<400>31 aactgacttc tgttccctaa gttatatctg tcggccttgc gggataccag tcattactgg61 gtgtatgctg ataggtatgt atatacattc atatcagttc tctaggtcat gttcctggac121 tccagattta tatctttaat tgcctagtga acaagttgct ttgacatccc aaaggcattt181 caaatgaaac tcatagatcc ctccaggctt gtttttttaa tctcggtaaa cgacactacc241 acctactcag ttacctaaga cccatcggta attcttgact ggcgctattt ttcctcaact301 atatctaata catttattcc tagtgaatct agctccttaa tatcactgga tattatctca361 atgtttccca ctttattgaa gaaacacagg tggtgtaaat cctggcatat gtccacatac421 ttaaccattc tatctcaggg aaggtggaat gacctatgat ttgtaaattt ggaaaggaca481 aactgggcat gctagtttct gccacccttc ctcctcgtgg ctgctggcac aggggacaca541 cat<210>4<211>423<212>DNA<213>Homo sapiens<400>41 ctattgcctt tgagcaggga aataattaca atgtctggca tcaaggcctg tgtagaatct61 tccacagatg ttattctgtc tcttggaaaa tatccacagt aataataaac aactaaagct121 tcattttaag tttaaagtat aaaaactagc tgtttcataa catagggacc aattctaact181 cctgactatt gtgaaatttt gtagggaatt ggaacaaaac tttggctggt tctacagcca241 aaacatgtag ggacttaaaa ttctagttag agataatcac tactttaaat gtagtgcctc301 ttctccctct tttgtgagct ggcagtcaga ggctggatta aggagatagg taagaggatt361 gcttcatact aatatgattg aatttaacac agattcaaaa tgcgagggaa tatagagacc421 tag<210>5<211>500<212>DNA<213>Homo sapiens<400>5
1 tgagacggat ctcactctct cgcccaggct ggagtgcagt ggcgtgatct cggctcactg61 caacctccac ctcccaggtt caagtgattc tgttgcctca gcttcctgag tagctgggac121 tacaggcacg tgccaccatg tccggctaat ttttttgtat ttttagtaga gacgcggttt181 cacaaccttg gccaggctgg tctcgaactc cttgtgatcc acccaccttg gcctcccaaa241 attctaggat tacaggcatg agccactgag cctggccttc aagcttcatt ttacaatcag301 tagacactgt cactaattat tgagaaaatt tatacaaatc aagggaaatt cagagaaaag361 tcaaacagaa gaaaaataat gagtctggaa gaaaatatag cccagccatt ccttctctcc421 aggcaggacc taagaagtgc tctctgtgga cacggcccaa accagacagg cccttcctgc481 ctgtgcccga tttcaagctg<210>6<211>500<212>DNA<213>Homo sapiens<400>61 tttaactttc tctcttttct gggccggaca tgtgcttatc taacatttgg ttctagattt61 atctaatggg gacttcagtt gtgagagata agtcagtagg ttttggtctg ccaaaagcaa121 ccattgtcca aaaaatcttg gccgccctca cctttctgag ttagataatc ttaactggct181 tgcattttct aattggatta acagctgatc tgagcttgga ttccatttat cactgtcctg241 cctggctctc ttctgttatc ttccatcttg ttgccttaat tctgtcttaa ttctaaaaat301 ttctcaggca catttccttt gggacacaag aagctcttgt atgcctatct ataattatag361 taacaactgg aaattcatcc aataattcct tgaatgtttt gctttcatct gatatttcct421 ctcaaattgt cctgactttc agggtacact tcgtatcact gtcccagtct ttgaagagca481 gttcccagtg gtttttatca<210>7<211>460<212>DNA<213>Homo sapiens<400>71 tttttttttt gcagaatgtg tcaacaaaaa atactttctt aatggcattt gtgttttaag61 aaaaatcagt cacatagtgt cagtgttaag aaaaggttgt aggtttacag gttgccccca121 gtggccatac tttgaattgt gattctcacg ggtttaaaga tgtgattttt aaaaaatcag181 tgttttacca tctagttatt tgatcttgtc cacttttttg tgttcaacaa aagtgtaaaa241 ctggaaagca aagaaaaaaa gactattttc agtaaacatg tagttttgtt ttgtctttat301 tgatagaatg taatgggcct ttgggatctt aagtactact tatgttacct ttgttattca361 gatgctgaac ttaagcagtg aaataataga aactaaagga tgttattaag gggtttaact421 attttaagtc aacatttagc ttatccatca tattggtttc
<210>8<211>448<212>DNA<213>Homo sapiens<400>81 ctacaagttc ctttattaat aaagcaaagt agaaaagaac aaggtcccca aatgacccac61 atgattcaaa gtgcaaattt actccaacct gcaagagaaa acgaatggca ggtcttgact121 gtgggggcca tggaaatttg gggtgattaa attgcctttg aatcatacaa ttgtagcagc181 tgaaccatat ctgattaact ctttggtctc tgttattgga acaaaaccga cgctatgcct241 gcagccgcca gactgcaacc aaaaacacag tttggggtca gaagacatta aaaatcacaa301 taaaatagga tgaatgttct aagtcacgca actgaatcaa ggcacctttt ttttttcaaa361 agcaaaaagt tgtttaacaa tattccagaa tagtagatac ttcaaaaacc agattacagt421 atatatcatt ttgctgcaca ttttagtc<210>9<211>384<212>DNA<213>Homo sapiens<400>91 tttttttttg ttgtcatata ttttattttt acatgtgcta cagaaacaaa atacagttac61 tatttttgcc ttacatagtc agataccttt tggagcaatt aaaaagaggg aaagcactcg121 gatcttggtt tctttcattc tccataaaaa taatgttcct tggagaaatg cctgattcta181 ggactggggc agaaaataca caagataagc ctagaatata ttatagtgcc ggaaaataaa241 aaagcgcctg ctccccctga aaaaaaaaaa cccacaaaaa taaggatata tcaaagggac301 ataggaatca agtagatagc cctcaaaagc tgaagctaga acaaagcaac agaaaaacgt361 ggtattgtat tataaaccaa agta<210>10<211>474<212>DNA<213>Homo sapiens<400>101 atcatatagt ctagtcttta ttgaaagact tacttcaact gttgtttaca gctttggctt61 aagttggcca ttttagcctc tcactgccag cagacataaa aatcaagtgg gaatggcacg121 ggggttggga gtgaaaatac tagtagagaa attatgagaa caaagaatat ggcagtcagt181 cagaaacaca tcaccaaaat aaatctcaaa ataattatga aataaaaaac atttccttgc241 atgcccaaaa agtctctgct gatgtttttg atgaacgggg tggggagcaa cgattcttag301 gcttaagttg gatcaagata gcattgggtc atgttataaa atcatctgtt agctcaacag361 ccatactttt aattaattca tttatttttt gagatggagt ctcgccccgt cgcccaggct
421 ggagtgcagt ggcgcacctt cagctcaccg caacctccac ctcccaggct caat<210>11<211>516<212>DNA<213>Homo sapiens<400>111 tttttttttt tttgacataa taataactct aacatttatt gagcttttgc ttaatgccag61 gaactgttct atagacatta tatgtattaa ctcacttaat cctcgtaaca atagtatgag121 acaggtattt ccattactct cattttacgt ctccacagta ataaagcaga gaaggagtac181 ataaccaccc aaagtcacta gaagaaggtg gaagaagcaa aattcaggtg gaggtccgag241 atgcttctct tccccactgt gtctgccacc tctagagtga atagccatga ccctgagata301 agtcagttac acacattgta ccaccgattt ctaaaacaac catatgagag aggtaccagt361 attatgccca ttttatagat gagaaaaagg aggctaggaa gggtgaagga attcatctat421 ggacagagga tttgtgccct ctcttttctc ttctacagag aaattgagcc aggatttaat481 cccagcatgc cggtttacaa agcccagctt tataac<210>12<211>415<212>DNA<213>Homo sapiens<400>121 ttgaatgtaa aggaaaactg caacttcttt aacaatacat tattcaaatt caaagatcat61 agcacatttt gagattcaga ttggtaatgt atctttgatt acactttgca aatgtatttg121 acaattaacc atatcccact actttttatt actgaggtag gaggcaggac tagactctgg181 aggtggggat tgggctccag accacactga agacaagctg aaagggaaga ggtgaaagag241 tctctccata aaacacactc accagtgcca cgtcaggtta ccattgctaa ggcaacagct301 ggaagttttg gctcctttcc atagcaacaa tcccacgacc tggaagttac gacctttttt361 ctagaaattt tggcataatc cacccctaaa tttgcatata gttgaaagtg ggtat<210>13<211>600<212>DNA<213>Homo sapiens<400>131 tttttttttc tgttgttaca tattttttag agatattggc ctattgttat attttggggg61 taggatggtc taatctttga taggttttgg tatcactgtt atcctggctg catgaaaaag121 aggctggaag cttttagtca ataaaaaccc aatatgaaca agtaaacgca gttattatta
181 cagtttagtt tatgaattaa acaggatttt gtgtgctgat gtagtaacca ccacttagac241 ttagtttcta agagaaaaat aaatcccaag tttcaaacaa ctgacttaca tgaaaatttc301 tggatgaata ttgtcggagt ctgtctttat cctgagattc tatagcaaaa ctcccttaag361 gattttaaag ggttttgaat acacccattt ccagccttcg tttttttatt taaaagggtc421 ttaattttca tagagaatct acacaatgca tgtataataa aaacatatgt cactgtcaaa481 tatagatgta aaaagcttat gttcttctat gttttaactc ttctctctag ctaatttgaa541 atatctctaa attcatgtcc aaactgagat aagacgttgg tacacttcaa taaaaatgta<210>14<211>381<212>DNA<213>Homo sapiens<400>141 gcggccgcct actactacta aattagcggc cggtcgacgt ccgaggggga agaggtttgt61 taatacgttc gccatgtgct acgatcttgg gacgaactga gccacgagcg tgcttgtgag121 ggccgtccga acgctgcagg ccggccaggt ccctgggcgt ccaggcctgg cctacgcacc181 actttgtccc ttagcgttta aaggtttctt cccgaatctc aggccctcag ctacctgcag241 gtttcgtcgc gagccggctg caagttttga acctaagtaa acctcaatcc ggaggcctag301 cggtaaggtg ggcgctgtgt ctattgaggt gcttagcaat aaagaaaggt agtgagttga361 aaaaaaaaaa agtcgacgcg g<210>15<211>695<212>DNA<213>Homo sapiens<400>151 tctctatctc agattttatt tttttttaaa aaaggctcca atacaaggcc ccagagtagt61 gcatgcaaat caacgaccct caaagtgaca gtcattgtca gataacaagc acaatctgct121 acagtctgtg acctggtaaa gagaccacct tttcattttg agtaccatca ccaactatgc181 caatgtccaa cctaccacac agctcacaat gactcagagg tacattttag tatctgaaca241 gcaaagacat attgagctgc aagtacaatg agtaagtatc cttagtgtat tagacagggt301 aaatacaaac aaggcataat agggaaaagc caacttcatc tgatgcctga atccctaagt361 ctcttccatc ttttctcatg atctgtgtct agggagatct gctatgccat atcaagacct421 tgctcatctg gtgatgagac ggggctgttg gcttggaaga ctaaagaagg gtgaatattc481 catgaggtaa gacttgaagt tgggttataa tcataggcag ttttgctgct gtcaaccatc541 tctgtactcc acttgggctg catgaatctg cgacatgtac agctgatggg agagaatcca601 ctcctgtgag tattccggag agatatcgtg gagattaatg gagatcttga gatacgcatg661 gtaacctatc aagattggtt agttggttgg tatct
<210>16<211>581<212>DNA<213>Homo sapiens<400>161 gccgactact actactacta aattggcggc cgctcgactt tttttttttt gtagataggg61 agtctaactg tgttctccag gctggcttca aactcctgac ctcaagtaat cctcccacct121 cagcctccca tagcactggg attacagcca tcacctacca ctccaagcca tgagtttggc181 tttggattta agaaggttga ggtgttcatg agttgacaag tggaaaaaac aagaaagaag241 ttgagtgttt aagactgctg tttgaaggag aagtctagcc tcaagacaaa agttcaggac301 tcatcagctg agaaatggcg ctgaaaatta tgcaaatgga tgagctcagc tagcaaacaa361 gtccagagag agcagcactg ggctatgcac ctggcctaat gcttccctgc tcctcccaat421 ccctgtgtta tgccggagag ggttcagcct ctggtgagct tcaccaaacc gccacatctc481 tttcttctga gaccttctct aaaatcccct cttttatgct tagtgaaatg ggattctctt541 tttcccatcc agcttaagca aaaacttttg actatgagaa g<210>17<211>560<212>DNA<213>Homo sapiens<400>171 tttttaaaga tcataatgct atcatttatt gaagaaagaa agcaaaaaaa actaacaatt61 gtgatggggg aggagagaca gcttggcaac attcctttta aagttaagtt agcataaaga121 aaacaatatt tagtaaatga aatagatttt caaggtagga aaagtagtac tcaagaatgt181 atttttggat acaaaaattc agttacacaa tcagtagcat tcaaaattag ttatgagtat241 ttatacaatt acaaaaatgg attcatgttt taacaaagta ttttaaaagc tcaaacattt301 taaaacaggc acaatattct aaaggcatat gcattcacca tgggcttttg aatgtcctca361 ctcccaactt cgcaatcaaa atctacagaa gcggcaaaag atcagagttc agagggctat421 tttttttttc cctttcctta cttaaggttg caaacccctt gggcggaggc aaaataaaca481 cgtttcatag ccgaaggacc caaaattgga tggaagcgta gctctccctt ggagattcac541 aatacaggtt catcggtctt<210>18<211>599<212>DNA<213>Homo sapiens<400>181 ttgtggcaca taatttgttt aatccagatt ggatacatca ggtacagcag tggcacacga61 ctcaatactg taaatgatat acatgtttta acatatgcac tacagtttca aaagaagacg
121 acaggaaact caagggtgta tttttttttt catagaaagt tttcatgttt tatcttcctg181 cagttttgta cagtatattt cttctttgcc attgtgatgt tgataagcaa agcccattta241 ttatacgaaa atagaaaaga acacacttga tgtatctcac gatgacatta atggggggaa301 gggtgtgacc tctaaggaaa acattacatt ggaatgtctg attatcacaa atataccatc361 cctcacacct cccaggtttg taaaatagtc cattggtcca aggagcaccc caggacttgg421 gtcagtgccg tacactggtt agagtgacat cctccagtgt agttgagtaa cctgctaaga481 taaatgtcat tcccaacttg agaaaaacac agacattctg aatagaaacc agacttaaag541 gaccctttaa gctgctcatt tctgaagcac ttcccccgtg cgaatcttgc ccggggcaa<210>19<211>500<212>DNA<213>Homo sapiens<400>191 cgaaaaagat aattgtttta atggaggctg tttaaaaagc agtggaagct tagcagagaa61 accagcgaaa cctgtgaggg tatctgaaga tgacttcacg gaggcggtac agctgagctg121 tggcttgatg gatgaagagg ggtttgctgg gtggaccagc gtgtttgctc caggctatgt181 tgaaatagta tgcaggttgg tcagggcagt atggtgccag tcaagggatg agtggggtct241 ggaatctagc ctcaagaggg ggaagtggca ccttttccta atttccacaa aggcacaata301 tgggctacca gtcttgagaa gtaggtgagg gcatgcaaat gtaaaaaagt aatgtgtgtt361 ctgggatcta ctagttcttg agaagcacaa gttcagaact gctgaagaac agagtgagag421 gtgatgagtt tgcccaggtt atggggccag gtcatgagag gcttttccat gaccgtgtgt481 agaaggcagg ttgggaatgg<210>20<211>564<212>DNA<213>Homo sapiens<400>201 taacaaaaaa ggggcccatg tctgataagt caagaccact tgtcctctct gagcttcttg61 agtaatggga gataattcca acgtcacaag gttgatgaaa aaaagcacca ttcatgtgcc121 tggcatctat ttattccact tcagttttct tcctatctgg ttgtctactt ttgctctcct181 acaccttgct caggggatat ccccagtact ggcaatatga gtgtgtttaa taaaggagca241 aaggaaagca ttttctggtg acaagtctca tctggctgtt cggtgacacc aaggcacatg301 cccaggaaac tggtagtttc cagtctggca ccacagcctc tagagaaaca gaaccagggc361 acctttccca gcaagtttat ggagaaaaaa cagggatggg caggtatcat caggaagcag421 ggtcccaggc tgagggcaga gatgtggctt gtttccctat ctagggatgc ctggggggaa481 ttagggtaaa aaggcaaaca gcaggtcaag gtagcttatt tgggccaagg ttgaagactg541 cagcctgaga cacttccagg ttgc
<210>21<211>388<212>DNA<213>Homo sapiens<400>211 ttgagatagc atcttgctct gtcacccagg cagaattgca gtggcacagt catggctcac61 tgaataatag atgttaaata atactagatg ttaaataata gtatcataag tacctacact121 gtttcctcaa ccctttgctt atatggtttc cttcatttga ttaaaaagct gaagtggcac181 atacatcccc ctttctgtca tagagaggca gatgacaagc ggcctaccca cggtttggga241 taatggacta gtggcaacag gcaagtccag cttttattgt ttgggatcct tactgagaag301 cagcaggctt cctctactgt cataaaaata ttaaaaagta agagcccgta taatttctca361 taataaaaca atgttttgcc ctcgtgcc<210>22<211>529<212>DNA<213>Homo sapiens<400>221 tattttgtaa tcaactgaaa gaaggcagaa ggagtcgatg ggcctcttag gcgagaaacc61 ttacaagcat aggaccaagg cagaaaaggt ccagagggtt catggtcctc cgttccacct121 gaatccagct agagagcgag ccagggggat aggtgtgccc ctcgtcgccc gtgcgctgag181 gcactgtcac gcaaagagac attcaccttc cacgtcaacg cacctttaag ggcgagacgg241 tccgccgtgc ccaagaggaa cgggatgaca ttcaactggg acttgcctca ccttggcttg301 ggggacctcg agagcggtcc cgtgggggca gtgttactcg tggtggtaga agtggagggc361 gtgtccgggt acttgagttc atgggcatct ctcccgccgc ctctcagcct atctgcacca421 tgtctcacac gttcagttgc agctcttacc gttttgaagg cgcacgtggg caagaagtcc481 tgggcagcac aagaaagtca atcacgttga gacagagaga gcaggagag<210>23<211>291<212>DNA<213>Homo sapiens<400>231 tttttttttt tttttttttt tttttttaag atgaataaat tgaagacatt tatttcctcc61 aagaaaaatg tctggcactt ggaattttca agctgcaagt agatgtacac attttcaaaa121 gatctttctt ttccttcgct tcctcacact gggcacagca aaacatctct gccttctttt181 cacgggaagt gaccctcctc cccatgggtc aataagttaa cgccaaatcg cggcaaaacg241 gcgaattcca tctctgaggc tctagaagct caatcttctg ggcccctggc t
<210>24<211>404<212>DNA<213>Homo sapiens<400>241 tttttttttt tttttgaggc agaattttgc tcttctcgcc caggctagag tgcaatagcg61 cgttcttggc tcactgcaac ctccgcctcc tgggttcaag caattctcct gcctcagcct121 cccgagtagc tgggattaca ggaaggcacc accatgcgtg gctaattttg tatttttagt181 agagacaagg tttctccatg ttggtcaggc tggtctcgaa ctcccaacct caggtgatcc241 acccgcctcg gcctcccaaa gtgctgggat taccagcgtg accnactgca cctggtcaag301 tatcatggtt ttttaatagt atgcacacat gggacaaaac tcaactggta taaaagggta361 tgcaggagaa aaaaagcaaa cttccctctc tcccttttct gtcc<210>25<211>379<212>DNA<213>Homo sapiens<400>251 ttttagtttg tagtaattta ttacagcagg cagaggaaac acacacaacc cttgaaccat61 cttctttagc gaaaattact aggagtttct cagtttcttc tgagggctgg tgtgacctca121 ggggccagca agaagagcct catccctcag ccacctttga ggaagtaaac atggacttcc181 tccctgagct gaatcagaac actccccaaa aactccttgg ttagccagaa aaatcctccc241 ttgtccttcc ataagccagc atgggtggga ttttccatca cttgcaacca agaaagtcct301 gactgagaag caacaggaga cggaggttaa ggtttccagg ttttaggctg gcttcccacg361 aggctgcaca gagatctcg<210>26<211>378<212>DNA<213>Homo sapiens<400>261 tttttttttt tgttgaataa tatacaacat ttatggataa ctcagcactt tacattcaat61 tcctcttatc cttaaagcag tcttgaagag tagatgctgt atgtctgtct tgcagatgaa121 cttagcattc ttctcatata ggcctcgcat ggcctggcca agtacttcct aagctcggaa181 caaatactgg tcaactttaa ttgaaccaaa tcgggcgggg tttgcggagt ctgagagtgc241 aacgttgggg agagggggat gaaaacactg gaggacgggc tgaaagcgtc gagtccgaca301 caaaagaggc gtcagacaaa acgccaagag gctggggact gggaacgaag gaagaggttc
361 tgccagaggc gacctgcc<210>27<211>389<212>DNA<213>Homo sapiens<400>271 aatttagaga tagttaccaa attacaaata gcttcacttt ggagttaagt aacaacctgg61 tttgaaattt cagctccatt acttattatc tgtgagactt tgaacaaatt agtaaaactc121 tctgagactg ttttcccccc atgtcaaaac acctattttg catggctata tggaaattaa181 atgaaatagc aaatatgaaa aacttttggc ccagtgtata attcatatca ggcactgatc241 tgttttcttt ttgccctaac ctacttttcc tgcatcatat accactacac ctctttacaa301 accttattaa tagagccact ctagactact ttatttcccc aagccataca atgtactttt361 gtgttttttc ttcagctaca tgctctttc<210>28<211>504<212>DNA<213>Homo sapiens<400>281 ggcgcctgga cccactttat tccgatattc cgcctgcaaa gcgtgtggaa agaagcgtaa61 ggctcaggga tggtagtccc agagaggagg gtccctgagg ctctgcgcaa gcccccctcc121 ccaacctttc gggccaagtc agacgaagcc tcggaaaggc tgggtctgtt ttgctgacca181 aatctccttg ttagccgttt ccgcctgaca agcctggcag caggcgttgg cttctcctct241 ccgggcggcc acctccaatg ccgcgcgggg agcaatgggg cccggcctgc agagggctgc301 ctggaccttg aagtttggaa aaacaagaag agactgagcc ggctccctcc cttctctgca361 gacacacacg caccgtcagc gcgccgccag cttcctgnct tttgtttttg cttcctccaa421 acatttattt gggcctcttg cattagccgc ggtggggctc tgggttgacg ataaaaggaa481 atacactctg aagacagttt tctg<210>29<211>500<212>DNA<213>Homo sapiens<400>291 ttttaaccac aaataattta ttactcagga gtgaatattt ttaaaattat gtagacattg61 cgattatagt acttagtctt ttcctaacaa aaagacaacc attctcaagt atcttattac121 agttattcat tgattaacaa ttaattgaac agcttctatg ttatagtccc taggcatatg
181 gcaatgaact agattgttat acaaagtccc tgctgccatg gagattacat tctacaggag241 tggacataga caataaatga ataaacaaat aaataaatga gataatttca gataatgata301 aagccttgaa gcccaggtga tagacaaaaa gtgaccatgg gatgttgggg gaagtttttt361 aaacattttt cttcttaaat ttggtttaaa taaacattta ccaaagtgat aaatatctgc421 agtttagtca gatagtacca taagtattta ttaatagcat ttattacaaa aaaacccaga481 aatctccatc tgcttcttcc<210>30<211>408<212>DNA<213>Homo sapiens<400>301 aatggagttt cgcccttgtt gccaaggctg gagagcaatg gtgtgatctc ggctcactgc61 aacctccatc tcccaggttc ccgcgattct cctgtttcag ccttccaagt agctgggatc121 acagccacgc accatcacgc ttgactaatt ttgtactttt agtagtgaca gggtttcacc181 atattggtca ggctgatctc aaactcctga cctcgggtgt tccgcccgcc tcagcctccc241 aaagtgctgg gattacaggc gtcacgacca tgcccagtct gggatctgtt taaggtaatg301 tgaaactatg acaaaccagg tattgccagc caatgataca agtggtgaac acttaacaga361 aaaagcagcg acaaatggtt atctacagga gaataatcag tttactac<210>31<211>533<212>DNA<213>Homo sapiens<400>311 taactgatga aacatacatt tattttttcc aatcaagtct taaaagtttg atgaaagcgc61 attattgtta ccaaaagtct tcaggcaata acagagataa aacttaacac agacaaacaa121 tgattttatt ccatagtctc ttggacttga gaatccattt gagtttcaga aagaatacta181 aattaatggg ggttatgtca ggatgccaat gtccatgctg aggcttctcc tgatacaatc241 ttttgcaaca taaccaacaa agatcaggag cttaaaacaa aacaaaacaa aaacaaacaa301 aaaacaagtt catgttattt ctacaatgtc caaaaagaaa gaccaagatc tttgcttaaa361 aatagaaatg catactgcag tggctcaaaa acttaggcct gcatcaaaat tacatgaagc421 gcttgttaaa acagattgag ccccatctgc aaagtttgtg actcaagtgt tgggtggggc481 caaataattt gtatttctaa caggtttcta agtgaagtca atgctactgg tgt<210>32<211>645<212>DNA<213>Homo sapiens
<400>321 tttagaaaac atatgcgcca tttattttgt tttataaact tttgttaaaa cacagaagag61 taagaaatgt tttttgtttc tttgccccat caggcggcag tgatgggggc tcaggggtca121 gcgcaagacc taatgacatt cacacccaca gaaagatgtg ggtcgttctg atgggaccca181 gcccgctttg ataattgctt gatgtaaatt tcacaattca tcttaatcag ggcagatcac241 agaaaaattt ggcaaaagta attggcttgg taatgacaag aaatagaaga gcgttcattt301 ctgaccctct cctctcctct gtccactctc aggagagccc tgcgaatttt ttagaagctc361 ctgagacccg cctgaagatc agggtgtctt ggtggggaaa ttgtgcccac gcttgtcccc421 ccagcaaccc tctgtttgtc catcagattt tatgttttag gaccacaact caagtagcca481 aaaaaaaaaa aaaaaaaaaa gagagcgaga gattttctaa tatcatgtca gcttagagtc541 aaaaaagtaa actttgtttt gtgtttctca cattacaata aagttattaa ggtggaatgg601 gtttctcctc cagaaagggg tagattccca gcagagaact gaccc<210>33<211>314<212>DNA<213>Homo sapiens<400>331 ctactactac taaattggcg gcgctcgact tttttttttt ttttggtaaa tattaacata61 gcaccaaatt ttgtaattaa gtataaatga ctatacattt gaatttagtt tagggcaaga121 ttttatacaa ggtcataaga ttaaatacag agttatttgc cccataaaat tagtactcag181 tactatactg aagacatact tttgcaaatc atcattcagc agaagttctt tatcccacag241 ctatatctat ctacaatttc aattttagga tctcaatgcc ctttaaaaaa ttgtatgggc301 caggcgaggt ggcc<210>34<211>640<212>DNA<213>Homo sapiens<400>341 ccagctgccg gtactgcccc tggacaggcc ggtcccccag cacgtcctca gccgccgagg61 agccatcagc ttcagctcca gctccgctct cttcggctgc cccaatcccc ggcagctctc121 tcagaggcgt ggagctattt cctatgacag ttctgatcag actgcattat acattcgtat181 gctaggagat gtacgtgtaa ggagccgagc aggatttgaa tcagaaagaa gaggttctca241 cccatatatt gattttcgta ttttccactc tcaatctgaa attgaagtgt ctgtctctgc301 aaggaatatc agaaggctac taagtttcca gcgatatctt agatcttcac gcttttttcg361 tggtactgcg gtttcaaatt ccctaaacat tttagatgat gattataatg gacaagccaa421 gtgtatgctg gaaaaagttg gaaattggag atttgagagc gttctatttg atagactaac481 aaatggaata ggccaggaaa cttaagcctt ccatgattta ggccttcagg ggttagatgg
541 gggctgccca tttgggaatg tgtgaactgc gggagatgtt gaggaagagg gtctggcccc601 taacagggac aaggtgtccg ttttgcagaa agccggtttt<210>35<211>643<212>DNA<213>Homo sapiens<400>351 ggtcgacttt tttttttttt tttctatgtg ggcccccata ggatggacaa tatctgccca61 aatggataag ggtggtcctt ccctactcag tctactagct caaatgctaa tctcttcgga121 aacaccctca cagacacagt cagaaataat atcttaccag gatctgggta tcctgtggcc181 cagtcatgct tacaacataa aatgaaccat cacagtcctc aaccaacact atctcgtata241 tccttaaaac agagaaacca aggttcagaa aagttaccta ctgtcacaaa agtaagtaaa301 cagctaaacc aaaaatcaaa ctcgtgtcta tctaaatcaa gagatcaaac acaaatgcca361 actaagattc tgtggaggag ataatagtta caagttaggc ccttgctcca agaaagttcc421 tgcagacgga gatcagattt atacacatcg aacaatgcga accctgtatt attctatatt481 gactacagct tatatacaaa gaagccttga ggaaagaaag tttcgggatt aaaccgattc541 cttcattcat ggatgcttct aagggttggt attgacagga cgggtaccac aggtcagcag601 tgtgttagaa atccacctgt ggccgctaac gggtggtggg aaa<210>36<211>383<212>DNA<213>Homo sapiens<400>361 tttttttttt tgccactgcc acctttatta tttagcactg ttatgaaaat atttaaacca61 tgcagtcaga aattatgtta taaattttga aaaggaaaac atataattat aacataattg121 ataggatttc tcacctggaa aaactcaaaa ggatcaaatg aatatctcta aaattaatag181 atttcaataa attgccaatt taaaataatc gatgcaaata aattcttctc ctatatagca241 aaaatgacca cataggtaac atattaaaag ctccaataag cagtatcaac gtgttccctg301 tatgtctgta ttacctagga acatatttat tacaaattat ataggattta agtaaaactc361 tcttgaagga tatataaagt ttt<210>37<211>474<212>DNA<213>Homo sapiens<400>37
1 gccatcatgc atcttatcaa atttaataac atgcacgcca gtcagagcac atttgtgggc61 aaacctgggc tatcagctgt cactttgatg cccaaggttt acatgtaaat tgggaagctc121 tgtggtttag aggaggaggg caggttgggg gagcaatgaa ctcagtccag cccatggccc181 acttagtcat ggactctcac agataggcag gatcttgggg aggtgggtcc atccttatct241 acattttaca gatggggaaa gccaaacttg tccaaggcca ccatggcaat gggccctggc301 attccctctc ttcacatgct tctcactcaa atttctgtcc tgggaaccaa gaaccttgag361 gatggactgc tccatactcc ttctgaagag agggtagggc agggtctgat tgggtggcag421 acactaggtc aggcccctcc ctcagatgct gtctgtccca gagtggcatc tggg<210>38<211>315<212>DNA<213>Homo sapiens<400>381 tttcaaatta ggttatttat tcttaaatat gaacaggaaa ctcagagtta tctggtattc61 aaggaaagcc cgccttatga atggcaaaga ctcaaattca aaacacaaaa gtaattctga121 aggaagagat aatttaaaga aaccacacaa acacagacta atgagagttt tcaaaaagtt181 tcaagaaatc ccacagttca caaaataaga atatgattat ttttaaaata acacttaaag241 aaatcctgaa aatgaatata tctgtggtat atgtgtgtgg gtatgcacaa tttgtgtgta301 ttaaatttga aaatg<210>39<211>498<212>DNA<213>Homo sapiens<400>391 gaaggcaggc taccgttgtc actactacta atttatctgc agtatgagta gcagtaggtg61 acttcaccat aactcacatc atctggatct caaagagcca ctctgatgac gtaagtataa121 tctggcagta tcgtgggaat caggaccacc gtctttgctg tagcaatacc cgggacctca181 gtttacatat ctcctttatg tgtcgagaaa gtgtatgtgt gggtggtagg agggccagga241 aaaaaagcta aaatcactca ccgtgtgatt gttcagcatg aactgtaccg cactaagctt301 cactagcttc cttacgctac tgtacccaat ggagaggtca ttgagaagct gtagtgtctt361 ggaggtgatt ggttcacaac ggccccagta cttcaagttg gtgatgcctg gaggacaaaa421 gaaaaaaaaa agcaggagac ccttttgatt tgctggtttc ctgagggctg ggagaagaag481 aaagtcattc atcaaaag<210>40<211>594<212>DNA
<213>Homo sapiens<400>401 aagtctgcac taaataactt acagtagcat ggcatagtaa gaaagaacgt tgtttcagga61 actgaagata taggttataa tctaagctat gtcattaacg aagtgtntat taagtaaata121 atttaagcat cttggtcatc atttattttc ttataaaatt aggagtttgt atcagatcat181 ctgtaattta cttcccagtt ttaaaaacaa atctggtctt ctgtcactct tgcaattcaa241 aaaccgtgtc tctgaagcag atcatgtgcg tagccagcct gtggtaggaa agtcagccct301 ccctcaggat gaaaggtgct tgcagaacct ccacccacac accttccaca caggggtttc361 tgatgctttt gcagaactga aagaattgga atagagatac actgatttcc acagaaattc421 taagggaatc actgtaaatt gtctaaaacc actttaagta ctgtgatcta acaaccacat481 tttcaaagtc acgttttttt ggactgcttt ggctgctatc tgctatggct gacagaattc541 tgaaacacac actagtgctt ttaaagtgtg cacgggaatc accagctcat tact<210>41<211>543<212>DNA<213>Homo sapiens<400>411 aatttggaac acaaatgctt tatttaaggg tgcatcacaa ttcccgtgtg gtgagcaaaa61 aataaaataa aggagcagat cgtagcacac cattcccact ggccaagtgg ttgaaccctg121 catccaagac ccagtgagca gccaagtgag gtgcaacctc tgggcctctc actcgtgact181 ccaacagaat tagcacattg cccttgtgca tggggccttt tacattgcag attttggagc241 agcaagacaa tgtatatctg ttcgtaactc ttgttcttcc ctcctgattt ttaaaactgc301 gtaattataa aactatgttg atggacatat aattattata aagatgtagt ttgtgacaat361 aacagcatgg gcagaatgaa ggcatataga gcaaattttt cattactgaa attaagttga421 gattaatctg aaacagataa aacagactag ttttacaacc tcctgtaata aaaacgctgt481 cttttaaaac aaattatcta ttgcactttt ttgtttttgg tgtcacagct atgaaagctt541 tgc<210>42<211>391<212>DNA<213>Homo sapiens<400>421 tgtttttttt tttccatatt tcttggctaa gcgactcatc tgtgaggttt ttcacaaaat61 tgttgccatc gccaaaaata ttccatttac tgaaaaaaat ccacatataa gtgtactcac121 gcagttcaaa ctaatgtggt tcaagagtca actgtataaa aggtattaat atgtctggaa181 agagatatgt ttccaattta acagacacta ccaatgagaa gagttgggct aggcaggaaa241 cttcaaattt tcctttttta ctttatatat tctatttgta tctcaactta taacctgtgg
301 accacgttta ttagaagaaa atgcagattc ctgaaacttc ctgcagaccc catgagtgac361 aatctcaggg agatggagtt ccagaatctg c<210>43<211>489<212>DNA<213>Homo sapiens<400>431 gttgttctca aacatgttta tttgtaacag ttttacatgg tgtgttacag aaattaatgg61 aaaatttcta aaaattttgc tgttctgttg aatgcattgt acataaagtt aaaaataatg121 tgtccatata tgttaacaaa ctgtcatctg aggtaaagtt aaagtaggtc tagaaaggta181 ctgcaacagt cattttctct ttctcatctt tattaaaaaa atatgtgtca gaacgcagaa241 attgaaatga accaaaccca cagagtaaac atttgtttaa aagaaggtgc ccagtacatt301 acatgatggg gtttcaggag acactgggat gagtacgagc tatcaaaaaa gtcttgcggc361 atgtaaagat tgaagagcta tggagaatgg ggacagaatg acaggtggtg tttccctggt421 ttcaaaattt aaagtgacaa atttagtgct agtggaggat gaggaattaa cagttgtaca481 catggattt<210>44<211>504<212>DNA<213>Homo sapiens<400>441 tagtctacac atgcattttt tttcattgct gtgcacttgg actcacttga agatgataac61 aaaataagga tgggacccag gcagcccttg gaagtttgct ctgtgaaaga ggatcaagta121 gatattccct ggcaggcttg tttactgatg gtgtctgctg ggccaatgca attaatttgc181 agcctggaaa cagcaggtct gtagttctca ctccaggagc ccctgtctac tggaagattg241 gctcccttga tgatggcatt gccttgactc ttctcaaagt cataactttg accttcagct301 ttatcttatc tcatcaattc aaattgcctg gtctaagaag tattcttagt agtttcaatt361 gaaagatctc ttaccccaaa ctctaaaagt tactttaaat aagaacttta tggacacttt421 ggtcttatat cctttcctta gaacaaacca aaagctatgt tcagttcatc tagcctatga481 gctgctcatg gtagactgga acca<210>45<211>529<212>DNA<213>Homo sapiens<400>45
1 ctgagaagtg tctcatacct tgcttttctt gtttcttaga agtgtcttat accagcatgt61 cagcttctta ctcataggat atggttaatg cttttttctt ctttaggtta ttttttatga121 gccctctgat ttcttgttct aattgcttat taggtcagct gcacaactgt cctactagta181 ttcctttcca ttgttcttct gggttaattc tataattttt gaatctattg tctccctctt241 tcttgaattc agttttcatt ttgccagagt atatcttcaa ggaatttttc agaaaatgtg301 cataacaggg gtggtggtgc accaaataaa tccaaatcct tgaacctcta aatgtgtctt361 tactatgcct tcacacttgt ataagagatt ccaaatgatt tcccttcaga atttgtagac421 attgctctgt taccttctag caagcccatt gcatatgaaa aatcaaatgc tactttgatt481 gttgtttctt tttaggtatc ctaaatattt tccttaagaa gttttaaga<210>46<211>482<212>DNA<213>Homo sapiens<400>461 cccagccagg aggatgatat aaaactgtgt tgacttcctt gccctcccaa ccctggaacc61 tggctgtctg tgctctgtta cactgtgact accattaatc accctcacta taaacgctgt121 tacacttaac catgcacatt gtcagcaggg tgaatcagaa gcaggaacag ttttgctgtt181 ttaaggaaac tctgtatatc tacaaggtaa atattcatgg aacaaatatt aagatgtcta241 ttttagcact gcctgtacat taagatgcag atgtgtgtgt gtatgagagt gtgtgtgtgt301 gtgagagtgt gttgtgtggg tgtgtatgtg tgtttgtggt gtatgtgggt gtggatgtgg361 ggtgtgtatt tctagtagaa tacttggaag aagagagact taaatctgga atcatgtttt421 tgtctctgca gtccaagctg gagacattaa aaacaacaac gtctccctca gttcaatcaa481 gc<210>47<211>438<212>DNA<213>Homo sapiens<400>471 tttccatgat ttcaattgtc attgttttat tttttccaaa ataacataaa aatactttcc61 agttgaatta cataaaaaat ttaatccata aaaccattat acagtcatgc attgggtaac121 aatgggaatg ggttctgatg aattgaaccc ttgggcgatt ttgtcacggg gaacatcata181 agagtgtact tacacaaacc tagatggtat agcctattgt tcctaggcta taagcttgta241 aagtgtgtta ctgtactgag tactataggc aactgtaaca cagtggtact tgtgtatcta301 aacaaatcta aacatagaaa aggtacaata aaaatatggt ataatggatt aaaaaaatgg361 tacacctgta cagggcactt accataaatg gagcttgcgc gactggaagc tgctctgggt421 gagtgactga gtgagtga
<210>48<211>403<212>DNA<213>Homo sapiens<400>481 actcactcat ccctcagtaa ggcaagttat ctccatttca cagctgaaga aatgaaggtt61 tggcaagttg ggtgacttgt ctaaaggcgt tcagtgggta agcagagagc cagggctgac121 cctcggtctg agcagttgaa acgtccagct gctttcctct gaccctgacc acctgggcat181 ggggatggag actgaagctt gttaagcagg aaggtgaccc aatctgaagt tttaaggaga241 tttccctgag gcagctcaga ggatgggctg agggcagtga ggtaggagag ggttggatga301 ttccagagaa accacccaga acactgaccc gaggcaggaa ctgtgggatg gacagaaggg361 gctggactgc agacactggg aaggtgtatt cagaattgct cgt<210>49<211>611<212>DNA<213>Homo sapiens<400>491 cttaaatatg tgactgggat actaacagaa aatgcagttt aatgtaatta aatagaaaag61 cattacagag tgggcacaat gaattgaata aggactttgt attctattta gcagtcatgc121 acattggtga ccaagaaagg atttacaatt cattgtcaaa aaatcctttt aaacggtcag181 tcttttaagg atggctgcag cagaacaagg caaactgaat actggaatga gatgggaaaa241 tcaacaccac ctaaagacta gttttgtgtt ttctcttggg agtcctctag aacagaattc301 agggtctact ttcatatttt ctgctatagt gctctatcaa gagcaggctg gggtggagaa361 catcaccaga ccatgcccac agccagggaa gagctgctcc ctaccaatta ccagggttgg421 gccagggcaa tttctggggc agagggagga ttgccatatg gaaaggaata taggacacat481 ggatgactca aactgtgatc tgtcatgaga naggcataga cgtggctact acttatgaga541 aaaaaatgag gattactaag tgaaaaatgt ctactctgtt tactgcacag tgcacgcgat601 gacaccatgt g<210>50<211>459<212>DNA<213>Homo sapiens<400>501 tttttttttg ctttttcaag ggcaacttta atcagaatag ccagctctac agacacatta61 cagaaaaagg aaaaccagtt ttttgtgtgt gtgttaggct attaaaaaaa tagtggtggg121 ttttttttcc ccattggctt ttttctctcc attatcatgc acgcctttct gttcaggatt181 gaggtcatcc atctgcaatc tcatatcctc ttgctgggtt ctgtcaagta ttagtcataa
241 ggcagtcagc ttgcttgtgt acacttcaac agatattttt cacatcaagc attgaagttg301 tatatgctta ttttagaagg gtggtcaaag tagcagatgg ttgcatggct attcataggc361 agataggcca agcatggcag gtggctcatg tctgatatca cagcactttg agaggccgaa421 gtgggaggat tgcttcagcc caggagttca aaaccagtc
權(quán)利要求
1.一類分離出的在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的序列,其特征在于,包括(a)SEQ ID No.1~SEQ ID No.50所示的序列;(b)SEQ ID No.1~SEQ ID No.50所示的序列中每條序列的互補(bǔ)序列;(c)與SEQ ID No.1~SEQ ID No.50所示的序列中每條序列有至少70%同源性的序列,及(d)上述(a)~(c)中一條或數(shù)條的組合。
2.根據(jù)權(quán)利要求1所述的一類分離出的在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的序列,其特征在于所述序列包括具有SEQ ID No.1~SEQ IDNo.50所示的序列。
3.一種探針分子,其特征在于所述的探針分子含有權(quán)利要求1中所述的序列中約8-100個(gè)連續(xù)的核苷酸。
全文摘要
本發(fā)明公開了一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的序列。利用本發(fā)明的在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽,可以方便的尋找出在人類肝臟中特異表達(dá)的表達(dá)序列標(biāo)簽,從而尋找出人類肝臟疾病相關(guān)基因,從而在研究肝臟疾病的致病機(jī)理以及開發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
文檔編號(hào)C12Q1/68GK1970757SQ20051011059
公開日2007年5月30日 申請(qǐng)日期2005年11月22日 優(yōu)先權(quán)日2005年11月22日
發(fā)明者韓澤廣, 黃健 申請(qǐng)人:上海人類基因組研究中心