專利名稱：人類肝臟表達序列標簽b組的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及生物技術(shù)領(lǐng)域，具體地，涉及一類表達序列標簽，尤其涉及一類人類肝臟表達序列標簽。
背景技術(shù)：
肝臟是人體內(nèi)最大的消化腺。也是體內(nèi)新陳代謝的中心站。據(jù)估計，在肝臟中發(fā)生的化學(xué)反應(yīng)有500種以上，實驗證明，動物在完全摘除肝臟后即使給予相應(yīng)的治療，最多也只能生存50多個小時。這說明肝臟是維持生命活動的一個必不可少的重要器官。肝臟的血流量極為豐富，約占心輸出量的1/4。每分鐘進入肝臟的血流量為1000-1200ml。肝臟的主要功能是進行糖的分解、貯存糖原；參與蛋白質(zhì)、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防御機能；制造凝血因子；調(diào)節(jié)血容量及水電解質(zhì)平衡；產(chǎn)生熱量等。在胚胎時期肝臟還有造血功能。
肝臟疫病分為肝炎、肝硬化、脂肪肝、肝癌等?，F(xiàn)代醫(yī)學(xué)實驗證明，肝病病毒侵入人體后，并不直接引起肝細胞的損害，只是在肝細胞內(nèi)吸收營養(yǎng)賴以生存，并在肝細胞內(nèi)復(fù)制、繁殖。其復(fù)制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細胞膜上，引起人體免疫系統(tǒng)對這些抗原物質(zhì)產(chǎn)生免疫反應(yīng)，這種反應(yīng)造成肝細胞的損傷、壞死。免疫反應(yīng)的強弱決定于肝臟受損程度及臨床癥狀輕重。這場由病毒引發(fā)的、免疫系統(tǒng)對肝細胞的戰(zhàn)爭，使大約25％的患者的肝臟成為戰(zhàn)火連綿的戰(zhàn)場，肝臟的損傷由此加重。肝病的危害絕不僅僅限于肝臟本身，它還可以引起其它多種疾病。常見的有(1)糖尿?。?2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎??；(6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結(jié)節(jié)性動脈炎；(12)消化性潰瘍；(13)自發(fā)性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對患者的身體甚至生命造成危害，而且對患者心理上的打擊也是十分沉重的。無論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學(xué)等方面都會受到嚴重影響。
生物基因組中可轉(zhuǎn)錄表達的序列(即基因)僅占總序列的3-5％，對這部分序列進行測定，將直接導(dǎo)致新基因的發(fā)現(xiàn)，并獲取基因組中與產(chǎn)業(yè)化關(guān)系最為密切的信息。20世紀80年代，高通量的自動測序的出現(xiàn)，使從質(zhì)?；パa脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫隨機選取許多cDNA克隆和決定來自非載體兩端的幾百個堿基的DNA序列成為可能。這些短的DNA序列叫做“表達序列標簽”(Expressed Sequence Tags，簡稱ESTs)。表達序列標簽的概念最早是由Adams等在1992年提出來的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.Nature Genetics，2，173-179)針對獲得大量信使核糖核酸(mRNA)序列的迫切需要，提出大規(guī)模互補脫氧核糖核酸(cDNA)測序的研究戰(zhàn)略。隨后Venter創(chuàng)立了大規(guī)模表達序列標簽技術(shù)。其基本特征就是從以質(zhì)粒為載體，構(gòu)建完成的目的組織互補脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫中，隨機選擇許多cDNA克隆，利用質(zhì)粒上攜帶的通用引物對cDNA兩端進行一輪脫氧核糖核酸序列測定，所獲得的來自3’端或5’端的幾百個堿基的非載體短脫氧核糖核酸(DNA)序列。簡而言之，表達序列標簽是來自表達基因片段3’端或5’端的短脫氧核糖核酸序列，代表一個表達基因的部分轉(zhuǎn)錄片段。
表達序列標簽可用于新基因克隆、人類基因組圖譜繪制、基因組序列編碼區(qū)的確定等。如果一個表達序列標簽在基因組中只出現(xiàn)一次，那么它可以作為序列標簽位點(STS)。由表達序列標簽構(gòu)建的物理圖譜叫表達圖或轉(zhuǎn)錄圖(expression ortranscript map)。利用表達序列標簽進行基因圖制作，可以加快序列標簽位點的制作和新基因的染色體定位。表達序列標簽可以作為基因特異性探針，對組織特異性基因表達的研究具有重要的作用。表達序列標簽還可以進行新基因的遺傳進化關(guān)系分析。表達序列標簽可以對所有動植物的基因作為一種數(shù)據(jù)庫，通過不同的序列比較可以獲得保守序列片段，從而獲得基因的遺傳進化圖譜。正因為表達序列標簽具有如此的優(yōu)越性，因此表達序列標簽測序已經(jīng)成為許多基因組研究機構(gòu)的工作重點。
由于本發(fā)明人類肝臟表達基因與一些肝臟疾病相關(guān)，因此，研究人類肝臟中表達的表達序列標簽對探索肝臟疾病的發(fā)病機理及研制肝病的治療藥物具有重要意義。

發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問題是提供一類人類肝臟表達序列標簽。
本發(fā)明要解決的技術(shù)問題通過如下技術(shù)方案實現(xiàn)本發(fā)明提供一類人類肝臟表達序列標簽的序列，其包括(a)SEQ ID No.1～SEQ ID No.50所示的序列；(b)SEQ ID No.1～SEQ ID No.50所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.50所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數(shù)條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.50所示的序列。
本發(fā)明還提供了一種探針分子，所述的探針分子含有上述序列中約8-100個連續(xù)的核苷酸。
由本發(fā)明的在人類肝臟中表達的表達序列標簽，可以方便的尋找出在人類肝臟中表達的相關(guān)基因，從而在研究肝臟疾病的致病機理以及開發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
具體實施例方式
下面結(jié)合具體實施例，進一步闡述本發(fā)明。應(yīng)理解，這些實施例僅用于說明本發(fā)明而不是限制本發(fā)明的范圍。下列實施例中未注明具體條件的實驗方法，通常按照常規(guī)條件如Sambrook等人，分子克隆實驗室手冊(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來源于5個成年男性，在肝臟切除手術(shù)后，將肝臟組織立即置于液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩后，再移入玻璃勻漿器內(nèi)，勻漿后移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑒定總RNA質(zhì)量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實施例2cDNA文庫的構(gòu)建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補平末端后，加含EcoRI切點的接頭。磷酸化EcoRI末端后，用XhoI限制性內(nèi)切酶消化1.5小時，再進行片斷分離。過柱篩選長度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，無菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進行包裝，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)細菌。涂板并測定滴度。
實施例3測序及數(shù)據(jù)庫建立(Seqencing and Database Constructing)挑選文庫中有外源片段插入的克隆，擴增后抽提質(zhì)粒(Qiagen Germany)，用T3和T7作為3’和5’端的通用引物，采用終止物熒光標記(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377測序儀(Perkin-Elmer，USA)上進行EST大規(guī)模測序。測序結(jié)果用FACTURA軟件去除載體序列，傳輸?shù)絊UN Ultra 450Server上進行下一步的處理。所有的序列信息再用GCG軟件包(Wisconsin group，USA)中的BLAST和FASTA軟件搜索已有的數(shù)據(jù)庫(Genebank+EMBL)，將無同源性或同源性低于95％的序列視為新基因建立數(shù)據(jù)庫。
實施例4基因的全長克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎(chǔ)上，進行cDNA全長克隆，分兩階段進行(1)“電子克隆”(Electronic Cloning)以新基因片段序列作為探針搜尋dbEST數(shù)據(jù)庫，將重疊序列＞50bp，同源性在98％以上的表達序列標簽(Expressed Sequence Tag，簡稱“EST”)序列認為同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER軟件進行連接，部分EST可以延伸探針序列。再用STRIDER軟件分析被延伸的序列是否具有完整的開放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和氨基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長完整性如何。通過電子克隆的方法，通?？色@取人肝臟相關(guān)基因的全長序列。
(2)cDNA末端快速擴增(Rapid Amplification of cDNA Ends，RACE)如果通過“電子克隆”方法仍未得到完整的cDNA全長，則在已有序列5’或3’端設(shè)計引物，在人類肝臟Marathon-Ready cDNA文庫(Clontech Lab，Inc，USA)中進行長距離PCR反應(yīng)。然后對PCR產(chǎn)物克隆、測序。用AUTOASSEMBLER及STRIDER軟件分析被延長的序列有無完整的ORF，如無，重復(fù)上述過程直至獲得全長。
(3)RT-PCR對于5’和3’端的已知的序列，如果中間有一段間隙(gap)無法從已有的公共數(shù)據(jù)庫或自身數(shù)據(jù)庫獲得，可考慮采用RT-PCR的方法。在序列5’端設(shè)計引物，3’端引物采用Oligo-dT，在肝臟總RNA庫中進行擴增。然后對產(chǎn)物進行克隆、測序。最后拼接便獲得全長。
通過組合使用上述3種方法，可獲得人肝臟相關(guān)蛋白的全長編碼序列。
序列表<110>上海人類基因組研究中心<120>人類肝臟表達序列標簽B組<130>NP-10047<160>50<210>1<211>598<212>DNA<213>Homo sapiens<400>11 ctaagaatac aaaaaattag ccgggcatgg tggcagacgc cgtaattcca gctactcatg61 aggctggggc gnacgaatcg cttgaacctg ggaggcagat gttgcagtga gtggagattg121 tgccacttca ctccagcctg ggtgacaagg ctgatgaatt catccgagca aatgccacca181 acaagctgac agtcatagct gagcaaatcc aacatttgca agaacaagcc aggaaggtac241 tggaagatgc tcacagagat gccaacctgc accatgtagc ttgtaatata gtgaaaaaac301 ctggcaacat ttactatctc tataaaccgg gagagttggt cagcagtatt tttccatcat361 ttctccaaag gaattggggg acaagtttgt ccacattgac ttccttggtg cctacaaact421 acagcatgac ttgtcctgga ctccgtatga ggacattgag aagcaagatg ctaaaaatca481 gcatgatggg acacgttgct aagccagtca gtggncctgc ctccgtgcac tgaacccact541 tccagggact gactcactga gagtgggctt tnacaaaaaa ggnttttcac aggacttn<210>2<211>490<212>DNA<213>Homo sapiens<400>21 catcacaaca cagagacaga caggagcccg ccctgcttta atgcgcatgg ggggagccga61 gacgagagct gctggcgggg gagccgagan gngngctgct ggcgggggag agcgtgtccg121 ggcgctgcac tctgcgcagg gctcttggat ggcgctcacc acgtcacgtc ttctntgctt181 tcggccaagc attgggccag gngntgtcaa catccagctg ggtcagcctg gggngaactc241 cttctggcac atggggcagc tgcgcagggc cgcggcaccc tccacagacg gctgctgctc301 caccctgggg gccctgcagg atcaggtgcc ggcgctgggn agggacctgg cgggggattc361 aggtgcctcc tgcccgtgaa caccggtaga acggccgggc ccacaggtcg gcggaaaggg421 ttcaggaaaa ggnttgggtc gacatgaaac ttagtggntt cgggcgcact ggntcctgtc
481 tgggaagcgg<210>3<211>533<212>DNA<213>Homo sapiens<400>31 cccgctgcca cctcgcccag ccatcaagcc ctccgatacc ttcggctaaa atatctttca61 tatttttaga atttgtcctc ggaaaccttt ttcgcttggg gtggtctctc tcactctgcc121 ccctcctcac gcagctcttg gcagtcaaca gacgctggcg gctggggctg ccatgccatc181 ccagctccaa gcttcccact ccgggacttg tgtttgggtg gggagacctg acctgggcat241 gttcctgttt cttcatcgtt gagcttttct ggcccggtct gaagctcaag tgaggagggg301 gaagctgggt ttttatcact tttaatgaat ttggtgtgat ttgttgtaga tttttaaaat361 ttcccctttt ggagaagaaa aaaccaaaaa aacttggccc actgggtaaa acatggggtc421 ntgggtccca agccctggtt nagcccctnc caagtttttt aagcctgaat gaaaggttgg481 ggtctctggg aacctggccc tcaatgccag aagcatcctg tggttgtana agt<210>4<211>405<212>DNA<213>Homo sapiens<400>41 atatagaaag ctacgagata ttctgaaaca gagagataac gaaatcaata tcctggtcaa61 catgttaaaa aaagaaaaga agaaagctca ggaggctctc cacttggctg gcatggatag121 acgtgaattc agacagtccc agagcccacc cttccgccta ggaaacccag aagaaggtca181 aagaatgcga ctatcctcag ctccctcaca ggcccaggac ttcagcattt tggggaaaag241 atccagtttg ctccacaaga aaataggaat gagagaggaa atgtcattag gatgccagga301 ggttttgaaa tcttcaagag ggaccacgct tgacaggttt accatcgntg ncatcaaaca361 ggtttctgaa acaggaggtt ttcttgaagn caaggncctg gnnga<210>5<211>410<212>DNA<213>Homo sapiens<400>51 caacagaagt tcaggttcgg gataagatca tcacagataa gaccaaggac acaatattca61 ctgatctcta agcaaacaga aaaactccac ttacagaaat cgttgcaact ttggaacttc121 caagacaatc tggcctctat caatgggcag acagcgaccc tcactgttgg cagaaaaaaa
181 aaaaacccag gnttttntaa cacaactgtt ataaantcac ggcaacaaan tttactccac241 ctctgantta ctggtcttaa tctcagaagc cgaatccgta cttatttttc ttcaggttat301 cagcttcggg ntaggtttca tcattgatta gaccaatcag nccttgtata tgtacacata361 atacattaaa ctgcacttct gtgacactga ctacaaccaa ggtantgcta<210>6<211>447<212>DNA<213>Homo sapiens<400>61 gctttaatta aagatctttt tttttttttt ttttttcant tataattgaa aatataattt61 gcataacaac agtaaattct acacatgang gcaaacgtcc gtggttgaca gaaattacac121 tggccagaat ccccagtccc catgaggctt gtccagacgc agtgaaccag tcgcagctga181 taacacacag accattcccg atcccagagg tgcatttcag gattcattct atttcatcag241 gtgacgtttt aacagaaaca aaaccgcagg cagcggttgg ggggagctgg aggttggant301 cacaccaaca ttntaggcat tnttactcta aaaaaaatta cttttttggg ctggggtgct361 gtgggctcac accttttaat tggggggggc caggcaggtg ggnttacctg aggtcggggn421 tttcggggac cagccttgac cnactgg<210>7<211>579<212>DNA<213>Homo sapiens<400>71 gcaatgccag agggaggctt gtgcaaatat tctcatagat tctggtgctg atccaaatat61 tgtagatgtg tatggcaaca cagctgtcca ttatgctgtt aacagtgaga atttgtcagt121 ggtggcaaaa ttgctgtcct gtggtacaga cattaaagtg aagaacaagg ctggccacac181 accactttta ttggccataa ggaaaagaag tgagcaaatt gtggaatttt tactgacaaa241 aaaatgcaaa tgcaaatgga gttgataagt ttaaatgcat tcatcaacaa cttttggaat301 ataaacaaaa gatatctaaa aattctcaaa atagtaatcc agaaggaaca tctgaaggaa361 cacctgacga ggctgcaccc ttggcggaaa gaacacctga cacggctgaa agcttggtgg421 aaagaacacc tgacgaatag gatacagtga attcctcttc aaagatttta gcctgtaaac481 atcctttaaa attcaagagg ggggaagatt aagtacaatg agttctgagt tcctcatcaa541 agaacaaata tgtcagtaaa aaaaaaaaaa aaaaaaaaa<210>8<211>1179<212>DNA<213>Homo sapiens
<400>81 acatcaatca acagtgttta ctaaaacagc aatacagaat tacaacattt tatgggaaag61 tgtcccattc attataacaa aaactttgtg gatttcataa aaatccacaa aatactcaag121 aacaaaccaa gaaatgagca catcggacat caaagaaatc ttaaaaattt tgccaaagct181 ctaaattcgt tgagagctgg aatccatttt caagtagtag gactctaagt cacatgaatg241 tcaattttcc aaactgtccc cataaactcc gtgcatccct taaaactccc gcaggactgc301 tgtgggtctt gacactgctt ctaaacttga catgggagag gaaacgtgta ggacccgctg361 aggaatgctt ctgaacggcc tcggggaggg agcctgctgt aaagctgtca cagttaatgc421 cagtgctact gtgcagaaag agaactcagc ccgtgagtcc ttgaaaccag ccctccgaac481 ataaataagc agccgggttc agtgaagcac atgacgcagg cttcacctct gccagcgttt541 ccatcccaga aagtcctgcg ggacagattc tcacagtccc cagtgagccc catccatgac601 ccctgctcgc cccccacgtt tcccagatga cggcagctgg agagccttcc tccgacagcg661 gccccaggcc acggagaggc ggccccgcag agagcggatg tttgtgaagc tcctcttgga721 gggaaagctg cccccaacgc tgaacactcc cgtctcaagg cacagacagg ggagcggtca781 caagccggcc agtccctttt acagccaaaa tcgggggctc agaacattct ttaatgattt841 ccgagccacc gagtgcactg ggcggccccg tctctgtttt cattccctga gatgtggtgc901 tttctctcct ggagccccgt gttggggacg cttcaaccgc aggccttgcc tccctcccac961 agtgctgagg aaggacaggc cctcccttcc ccaggaaagg ccaggctgga ggagcccagg1021 tgtgggggga agttctgagc tggcattgtc acccccgggt ctagtatccc cacagagtgg1081 accctggccc tgagcccagg ctgtgcctcc cctatgtgca gccgctggga tgtgtccagg1141 gcagcacagg aggcaggtgc ccaggtactc ccctaggca<210>9<211>1284<212>DNA<213>Homo sapiens<400>91 cagggaagca gaggagcagc agggtcaggg tgctgggttc ctaaggtgca aggatgcaga61 acagaactgg cctcattctc tgtgctcttg ccctcctgat gggtttcctg atggtctgcc121 tgggggcctt cttcatttcc tggggctcca tattcgactg tcaggggagc ctgattgcgg181 cctatttgct tctgcctctg gggtttgtga tccttctgag tggaattttc tggagcaact241 atcgccaggt gactgaaagc aaaggagtgt tgaggcacat gctccgacaa caccttgctc301 atggggccct gcccgtggcc acagtagaca ggccagactt ttaccctcca gcttatgaag361 agagccttga ggtggaaaag cagagctgtc ctgcagagag agaggcctct ggcattcctc421 cacctctata tacagagacg ggcctggaat tccaggatgg aaatgactcc cacccagagg481 ccccaccatc ttatagagag tccatagccg gcctggtggt gacagcaatc tcagaggacg541 cccagaggcg aggccaagag tgctgaggca gagaaagctc tccagcactc atgatgccac601 cactgtgggg agcagctact gataataaag gcaaacgagg gactgtggga ggaaaagcca661 caccagggac cccacaagag agcttgggga ggggatgaca gccctgaatt ccagacataa721 ctggccatgc cagggcaggg caggccaggg gccctggaag ggtgtggcca acaccaagca781 gacatttcag aggattcaca ctaatcttaa ccccggctgg ccttgggtcc ccaaaggtgt
841 cactctgaga gctctgctta cagagattct ttctgctgtc actcaatttc cctcccatca901 cctcaactcc agccagggag gaatagatgc tggttgtcag gacagccaca tgccttcctg961 ttctatatca cctagtaatc tcaaagcgaa ggtttgttgt ttaatttatt gattgttcag1021 ccttttgttt tgttagcttg tttcctagtt acctccagct gaccatacta ccagagtgag1081 gcagtacttc acatcctggg agatatgccc tcggaaccca tgaatgttat tttgtattat1141 tttattttca tgatatgtat tttttctatg taaaaggaaa atgactgtaa tatttgcctt1201 caagatggtt caaacacaaa atattaaatg agctgtttct taaaaaaaaa aaaaaaaaaa1261 aaaaaaaaaa aaaaaaaaaa aaaa<210>10<211>503<212>DNA<213>Homo sapiens<400>101 ctcatcagtt tttaaatttt atttaatttt tatttatttt ttttttaaat gtaatttttt61 cagagagata aggtcttgct atgttaccca gcctagtctt gaactcctgg cctcaagtga121 tcctcctgcc ttggcctccc atgctgctgg gattacaggt gtgaactacc atgcccagcc181 agctcctaag tcttaaggct ctgtgttagt gatagatgtg gccatggtgt aggcagtgca241 atgtcttcga gtgagagtga aggtggtaac tcattgcatg gattctagag ttctgtttat301 tctaatccaa gttcttccac ttaaaaacaa tgttcttcct ctcattgagt ctcattcctc361 atctatagga tgggaataag agcatgtacc tggcaggttg ttgtaaggat taaatggtgt421 aaacaaaatg tcaagtgctt gcaactttga ataccaaact tgagtgaaag ctcaataaat481 tgttacttaa aaaaaaaaaa aaa<210>11<211>604<212>DNA<213>Homo sapiens<400>111 tttcatgaag accagtttat tttacatgct tgctttcaca ttctttactg ggaatttaag61 gccttttttc agccttaact tgtataccaa cctcaaggat tttgtttgat acagaaaagg121 atagggctgg gcttctgcca aggactgata acctgcctgc caaaaggaag agggaatgaa181 agccttttgt ccttctaggc cccttacagt acctcaaaat ctaaaggcct taaaggggaa241 aaaaaccgta tctgttcttt ctccttatct cctacccttc tctttaagca tattgaagat301 ggactttttt ccaaatgttt atttgtagga agaggtgatg agtgcaggcc agcagctgga361 gaactttaca gctttgatgc accaggaact gtattcaagc tgagggcaaa agcctcctag421 ggagggagcc aggtccacca aggccagaga cagacaggcg agactgtgga aggccaggga481 gatgctgcct ggtaagtgct ccgctggnct actgggcaag tccnccgggg gntctagagc541 tgataggaag aaggagtcca ttttgaagtc ctgcctggag acttngacct agctgaacta601 aggt
<210>12<211>1472<212>DNA<213>Homo sapiens<400>121 gtgtgggcgg ggcgcagttg ggggagggtg cagagacctg agggcttgag gttgcctggc61 tggccccgct cccagaggcg ggtgccgcgc tgtcgcccag gtatctgggg tctctggtgt121 ctgagtgtct cattgtcggc gcgaacacaa ttgctccagc cacaggcgag gcctggccaa181 ggtgtgggcg catctagggc aggtcttgag aggtccagcg cccggtggtg cggacagagg241 cggggcaccg cggcgctcgc cgccgcctcc ccgcaggtga tcatcctcct gcaggtgtcc301 tcgggtctca ggtggctgcg tgtctgcgcc atggttgaca ttctgggcga gcggcacctg361 gtgacctgta agggcgcgac ggtggaggcc gaggcggcgc tgcagaacaa ggtggtggca421 ctgtacttcg cggcggcccg gtgcgcgccg agccgcgact tcacgccgct gctctgcgac481 ttctatacgg cgctggtggc cgaggcgcgg cggcccgcgc ccttcgaagt ggtcttcgtg541 tcagccgacg gcagctgcca ggagatgctg gacttcatgc gcgagctgca tggcgcctgg601 ctggcgctgc ccttccacga cccctaccgg caacggagtc tcgctctgtt gcccaggctg661 gagtgcagtg gcgtgatctt agctcactgc aacctttgcc tcctgggttc aagtgattct721 ctagccttag cctcctgagc atctgggact acagccattg ctgtgaatta cgtgagggaa781 agatattgaa gaggagttgg acactccgag agtgcagctg ttctcccccc gcaccatccg841 tgtcctgcat tctgcgagtc tgtgctcatt aacaatgtgc tgtgaccatg tgactcagca901 atcctgctgc tgggtatata cccgaaagaa aggaaaagga agccagtata ttgaagaggt961 atctgcaccc ccatgtttat tgcagcactg ttcacaacag ccaagatttg gaagcaacct1021 aagtgtccat caacagatga atggataaag aaaacgtggt acatatacac aatggagtac1081 tcttcagcca ttaaaaaaat gagattctgt catttgcaat aatatagatg gaaaaggagg1141 cccttatgtg aagtgaaata agccaggcac agaaagacaa acatcacatg ttctcactta1201 tttgtgggat ctaatgatca aaacaattga actcttggac atagagagta gaaggttggt1261 taccagaagc tggaaaggaa agtggggttg ggaggaaggt gggaatggtt aataggtaca1321 aaaaaataca aagaataaat aagacctaat atttgatagc acaacagtgt gactactgtc1381 aataatcatt taattgtaca tttaaaaata actataattg cattgtttgt aacacaaaag1441 ataaatgctt gaggagaaaa aaaaaaaaaa aa<210>13<211>784<212>DNA<213>Homo sapiens<400>131 gctgctaggg ggcggcttgg ttctgctgct gcaccacaag aagtactcag agtaccagtc61 cataaattaa ggcccgctct ctggagggaa ggacattact gaacctgtct tgctgtgcct121 cgaaactctg gaggttggag catcaagttc cagccggccc cttcactccc ccatcttgct181 ttttctgtgg aacctcagag gccagcctcg acttcctgga gacccccagg tggggcttcc241 ttcatacttt gtgggggact ttggaggcgg gcaggggaca gggctatttg ataaggtccc
301 cttggtgtgc cttcttgcat ctccacacat ttccctgggg atgggacttg caggcctaaa361 tgagaggcat tcttgactgg ttggctgccc tggaagggca acgaaaaata gatttatttt421 ttttcacagg gaaaaacaaa caacaaaaac taaacaagac aaaatcgaca ataacaacga481 caacaaacac acacacacac aaaaaactat acacaccaac agaactacaa ccaacaaccc541 acccaacgcg cggagagaaa acgaacggag acaaggggga cgccgcccga acgccccgga601 atggatccgc aacaacaccc cagcgagggg gcaagcccga cgaagacaga cgaacgcgac661 acgtcacgcc agagaacagc gcgaacagga gaagagcgag caccaccaaa aagcgcggcg721 gggaaagacc tcgtgaacac cgaggggtgc gagccgacgg gcaccagagg ccagacaaaa781 cccg<210>14<211>399<212>DNA<213>Homo sapiens<400>141 gaaggtgctt tagtctgaag atggcggcct cagcagcgcg aggtgctgcg gcgctgcgta61 gaagtatcaa tcagccggtt gcttttgtga gaagaattcc ttggactgcg gcgtcgagtc121 agctgaaaga acactttgca cagttcggcc atgtcagaag gtgcatttta ccttttgaca181 aggagactgg ctttcacaga ggtttgggtt gggttcagtt ttcttcagaa gaaggacttc241 ggaatgcact acaacaggaa aatcatatta tagatggagt aaaggtccag gttcacacta301 gaaggccaaa acttccgcaa acatctgatg atgaaaagaa agatttttga gactgcagcc361 tattaataaa gttaacataa ctgagaaaaa aaaaaaaaa<210>15<211>631<212>DNA<213>Homo sapiens<400>151 acacaaagaa aaagatttta ttgtcttctt agtcaatatc cctggtgaaa ttagaggcat61 agcttgagac tggtgacagt gcaacacaga ccttcaggag ctgctttgag gactggcctg121 cccagatgcc tgctgttaan cagcagcccc ctcactccgg cccctgccat cttgacagat181 ggagctgcca tggtttcagg gacactcagc agggcatctg ggttggtccc tcccacatgg241 accttgtaaa gttgctattc aggggaacct ggtatcgttt caggcaaaac acagaaccat301 attagcactt ctaagccccc tgccccggnc gcctccccgg aacatttggg cttgtcgcac361 attccaggag ggagcaggag cacagctgca gccacagtnc caggaacagg cctgggctcc421 cgcctgtgtg gggggagggc agaggctgag ggcttcctct gggggccctg agtaagctca481 ccctcanccc acctctctga cttcatccat ccaaggccgc ttgcaagagc ccaggcaagg541 tgaaggggct gtntccagac aagcctgggt tgcctagcaa angtggggaa ggggaacttc601 aggctagcca aatctccaga acnagggtta g
<210>16<211>1348<212>DNA<213>Homo sapiens<400>161 gcgagctcgg cttcctcaac atggctgcgc ccttgtcagt ggaggtggag ttcggaggtg61 gtgcggagct cctgtttgac ggtattaaga aacatcgagt cactttgcct ggacaggagg121 aaccctggga catccggaac ctgctcatct ggatcaagaa gaatttgcta aaagagcggc181 cagagttgtt catccaggga gacagcgtgc ggccaggaat tctggtgctg attaacgatg241 ccgactggga gctactgggt gagctggact accagcttca ggaccaggac agcgtcctct301 tcatctccac tctgcacggc ggctgagggc ccttctctgg gcctgggcac ccttagaggg361 gagaacgaag caatcagaca tccccttggg ccctgcttcc aggtctccct gtcccccttg421 cctgccttct tccctgctct gtcccctaag ctccctccag gcagggaaaa gaggccaggt481 gctaaaaatg agcctttctc aagcacgtga gcagcggaag gcagacaggc gccagagccc541 agcactccct tttccagcag ctgtggtggg ggagggttcc cctccagttt gtcaagagtt601 gaaggaggct ctgtggccag gtgacctggc tgccttccac cccttgtacc tcagtctaaa661 catggagtgg ccgctgacaa ggcgctccag ccccagagcc agcgtcttca tggggaagat721 gaatggacct gagtagctga aggaaggccc ctccctaccc aaagactgga ggcttctcag781 cctcaatttc cctgtctgta cagctgaggg ctctgcctgt cccccactgc tatcagtatg841 gaaccccagc tggggtcccc tattgagtgc cgactccccc caccgccagc agctgctcct901 ccagccacac ccctcctgct ccccccaccc ctagcccttg accctggctg gcctgccccg961 ctccacaggc caccagatgg gctcctgaga ccctccccag gctgcttaca gctcattctg1021 ctgggggtag agatgagggg agggagtaag ttaaaccttg gactagcaag tagaagcctg1081 gggggatgcg tgtgcctcag tttcctcctc cacaactgaa tatttatagt ggctgaaaac1141 tggggagata cttgatggcg cgaatgtccg ttttctctcc cttcccacct cctgcaggaa1201 gcaggacggg gcaggcagca cctggtaggc acagtgcttt gcccctcctc cccttccctt1261 ctggaagtct tggggcctca gtgcttgcaa cagccggcct tgggcaaata aaagactagg1321 ttgtttacta aaaaaaaaaa aaaaaaaa<210>17<211>1808<212>DNA<213>Homo sapiens<400>171 ataaaaagaa tggctttgca ctgagcagct cagtctactg gtctcatcag tgaaaggctt61 gtgaaaattt ctggaaagag cattggctgg cttgttcatc tctctgtttg gtcagttgct121 gtgtttctgc cggatcagat gagcagattg gttagtgaag gtcagtgtga gaagaagatt181 gtttctgagc ttgagaagct tctggaggat tgaagagtat ttgaagtctg tgtcaaacat241 ccacatcata agtggaattt tggagatatt caaagaatgc tgtcagaagg gtatctcagt301 ggacttgagt actggaatga catccactgg agttgtgcct cttataatga gcaggtggct
361 ggggaaaagg aagaggagac aaattctgtt gctacccttt cctattcctc tgtggatgaa421 acacaagtca gaagtctcta cgtgagctgc aaatcatctg gcaagtttat ctcttcagtg481 cattcaagag agagccaaca tagcagaagt cagagagtca cagtgctgca gacaaacccc541 aatcctgtgt ttgaaagccc aaacttggct gcagttgaaa tatgtagaga tgccagcaga601 gagacctact tggttccatc ttcttgcaaa agtatttgca agaactataa tgacttacag661 attgcagggg gccaggtgat ggccattaat tcagtgacaa cagattttcc ctctgagagc721 agttttgaat atggcccttt gctgaagtca tctgagattc ctttacccat ggaggattcc781 atttctactc agcccagtga ctttccccaa aaacctatcc agcggtactc atcctattgg841 agaataacaa gcatcaaaga gaaaagcagc ttgcaaatgc agaatcctat ttctaatgca901 gtgctgaatg agtacctgga gcagaaggtt gtggagttat ataaacagta cattatggac961 accgtgtttc atgacagttc tcctacccag attctggcgt ctgaactcat catgacaagt1021 gtagaccaaa tcagtcttca agtgtctaga gagaagaatc tggagacctc aaaagccaga1081 gatatagtct ttagccgcct attgcaattg atgtcaactg aaattactga aattagcact1141 cctagtctcc atatttctca gtatagcaat gtaaatccat agagaggatg cttccattac1201 tgtctcgcat ttacttaaac atgaagcaac actttatcca tttattctga gaatgtgcag1261 gaggggttag tgaaggggaa ttaagggcta gagaagtaaa gatcagctgg aagtcatgtg1321 tgaatcatgg agaaatctca taatattaca cttgatgaag gaatatggta agaggagcca1381 taaggaatga tttcaaagag aggtgtgtac agcaaggaag caaaaataaa aatgatcaaa1441 aaagaaacgg ttaattcatt tgaagaaaca taggaattaa aaaagaagac agtcaaatga1501 gatgaaacaa aaaggccaaa aggtagccgg gtgtagtggc tcacgcctgt aatcccagca1561 tttgggaggc tgatgtgggt ggattacctg acgtcaggaa ttcgagaccg gcctggccaa1621 tatggtgaaa ccccatctct actaaaaata caaaaaatta gctggacatg gtggtgggta1681 cctgtaatcc cagctactag ggaggctgag gcaggagaat ctcttgaacc tgagaggcag1741 aggttgcagt gagccgaggt cgctccattg cactccagcc tgggcaacaa gagtgagact1801 cccgtctc<210>18<211>508<212>DNA<213>Homo sapiens<400>181 ctcggcttta ctgcagcctc cgcttcctgg gttcaagcga ttctcctgcc tcagcctccc61 gagtagctgg gactacaggc gcgtgtgcca cgccggctaa tttttngtat ttttagtaga121 gatggagttt caccatgtta cccaggatgg tctcaatatc ctgagttcat gatccaccca181 ccttggcctc ccaaagtgct gggattacag gcgtgacacn cacacccagc cagttttcct241 attttctgaa ttcagaattg acttctctgg gaaaactgga gatgagaatc tgcccagtgc301 tctgctgtcc agtcaccgcc ttttgaattt tagttttggg caccagggag taccgttagc361 tttccccttc tttctggccc atttgactca tcagcacaat aggggtcaac acctttaact421 ttttaatttc taantttgtt ttattgtctt taggggtgtg tatggaaata aaacattgaa481 cagggtttct tggaggccct caaggtgt
<210>19<211>712<212>DNA<213>Homo sapiens<400>191 tttttttttt tttttcttaa aactcaagga ttctttatac tcagataagg ggtattctca61 agtaccaata gcatcacaat ccaagattga taaccttgaa gtgaggacat gggttcagat121 ttggcttcat catggggcca aattcctgac cctctctgga tttcagtcct ggtctggaaa181 actggacaac acataactgc ttcatttggc tactgtgaga actgagtgag ctctgctatg241 tgtaagggaa accagcaatc atcctcataa acatcaaact tgggcccaaa gccagcaagg301 gagagagtct ccagatgggg agggaagagg ccagacctca tggcctcaag tcctctcttc361 tgagtccttt cttccccttg gtggtggtag tggggatatt tttcatgaat taccacttgg421 aggacctggc ttgatttatt atacagggag ccgatagttt tcctaacaca agtggtcaga481 ggtacagcag ttctgcttgg ccgagctgtt gaaggagact gttctcagag ctcctccctc541 tgtgatcttt ttgaggaagc gaggagaggt gtgaaagtgc ttttanaccg tcaactgggg601 ttcctgtggg aggagttacc ctcaatgacg gtccataata agctcatgaa ggggcatttg661 gagcagccac gacactcagt gcacccttgt gtggggcagc cctgccctgg gc<210>20<211>675<212>DNA<213>Homo sapiens<400>201 ccacaaagta gattatctaa ttaccatcaa aatgagtcag aagcaaaaaa caacaccctc61 caacagcctc ttaggagtac accctgttct gctactaaaa tactagactc atttccctgg121 tggtgggggg gaatgcaggt atagatcaga aatataatcc accttaaaat tagtactaat181 gatcacatta tttcacaaga actctgctgg atgtacagct cttaacattt tattaagcca241 tttgtgtagc ccaccacgtt tctttgtggt gcttgtcctg tgtggaagat tgtaaatctt301 gggcctgttg catacttcca agggctttaa agcaatgtga ttaacagtac ttggcctgct361 gcttttggct tactttgtat ttttagctag atgctattat agtgttgaaa ggatatggat421 tctgtctggg atgcatttgt tgtcctttca gaacccttcc tcccattacc ctgtgaataa481 aaacaaatgg gaccccctga gaaggacatc ttcctacagt gatcacctca ccagcccagt541 gttgtggctt tgtagcgcac cccatatcta ccatattcta gaacactgta atgctactag601 gctggcccag aaaaggttta aatgttgaat tcacttaaat tgcaattgtt gcaccatcat661 ggaatcgtga tgtgt<210>21<211>693<212>DNA<213>Homo sapiens
<400>211 gggagtacgg gatggtaaga gctgggttcc gacagcagct cagcttaacc ctctgtcctg61 ctaacgaggg aaagtgcggc ggctccgcgc ccagcacgcc cggtaggccg gagagagcgg121 cgaggccttc ggtgagcgaa gagttaaccg cggcggagcg acagatcgcg gagctgcacg181 ctgccgcctg cgcggctggc cagctaaact atgtggatcc agctactggc tatgtggtgc241 tcacacagat tgcccacttg caaagaggtg aatgttgtgg ctctgcgtgc agacattgtc301 catatggtca agtcaatgtt aaagatccat ctaaaaagaa gcaattcaat tcatattttt361 atgtttgaca agaatttcat ctctgttccc taactgtgct tgtatttttt aaaaaataaa421 gccccaattc agaattgctg gattattagt acttgaacac tagtttaatc ctaaatacat481 atattaaaag aacatcaata aaatgaaaaa gctaagcaaa aaaaaaaaaa aaaaaaaaca541 caacaaaaaa tcagacaaca aacacaaaca acaataaaac acaaaagaaa gagggggggg601 gagcccataa ataccacccc ggggggggca cccattccag gcaccccgcg gtttttcgaa661 cagggggccc ccaccatagg ggcgcactat acg<210>22<211>635<212>DNA<213>Homo sapiens<400>221 gtggggggag ggagggtcca ttccaccagc tttgaatccc aggtcccctc agcttcactg61 ggccacagtg tctgtcccag gaagaagcaa atggacagtg aggatgatgg agagactaca121 gctgccttgt ctaaaacctg ggatcttaaa agtgttattt tggcttttat tttttaaaaa181 aagaaaagga aaattggcaa ttgtattaat ccattctcga attgctgtaa agaaatacct241 gagactgggt aatttaatcc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ttttgggggg301 cagccgaggg accctggaaa agagccttca aagacacaaa tgcggtgggg caggcgcggg361 gccccaaaaa ggtaagaggg aaacagggga caaaaagggt gtcccaagga gaaaactcgc421 gggaccagaa ccaggggccg ggaaatacca ccacaattat aaaaaaaagg gccgcggggg481 ggcaaacgcg gagggagtcg acgaaaacaa aatccaaaag agggagaatc tcgggaaggg541 aacaaaagag gggacatcct aacacagaga gaggaggtca ccagagggga agcccgagca601 ataaacaaaa aataggcgcc cgcaccggtg ggggg<210>23<211>802<212>DNA<213>Homo sapiens<400>231 caatgcattt tatatatttt tagaaaccaa ataaatgcag aaaactattt agtatactaa
61 ttatattaaa ccagcagaaa tataaaggca ataaggtata tacatatata tggggggagg121 ggaaagatta aaaaatagat ttacagccag acatggtgat agccctaaat gtatttctca181 atatgttttt ctttattgac cctaggagga tttgagttgc tgcagcttta aacagaaaat241 tgccatgttc actaattgtg agcacataaa tatgctttta gtactgcttt gcttatgtac301 aaataaatct gtaatatgta ttatatgtaa ttatcctttt ctttgacttt cattttaaat361 gtatattttc tgatcattat gagctgtaaa acaaagaatc aatattggat attctcccaa421 ataataaatt ttcaggattc attgggcgaa acaacaacaa acacaaaaac acatacacaa481 cataataatt caaaaaaaca actcgttttt gcgcgcgcac actgcggccc ctcgaaaaaa541 ccgtctcttc gaaaacccac tctgctgttg cgggcgcgcg ggaccccccg atgtggataa601 ggggaaccac ggtgcaccaa acgagggcgt gtctccccaa tgggaaaaac cgcggtgggc661 acgaaaagca agtggcggtc ggatttccgt ccacccaagt tataaaaaat cggccgggcg721 taacacccgt gagctgtttc gtgacacaaa gcccccaaaa ggggctcact cagagacgga781 tcaaaacggg ggaaccacac ag<210>24<211>528<212>DNA<213>Homo sapiens<400>241 gttgctacgg agtgcatcgg acgtcgaagc ctagagtctc tgcgtctttc actcttccgc61 tgctcattcc tttccttcct agccttggtc gtcgccgcac catgaaccaa gaagaagaaa121 ccgttcctag ggaatgcccg cgccctcggt acgtgccggg gctgggccgg ggcgccactg181 gttcaccacg cggtctgaca tgggcccgcc gtgatgcaaa tgaccctgtg gatgatcgcc241 atgcaccccc aggcaagaga accgttgggg accagatgaa gaaaaatcag gctgctgacg301 atgacgacga ggatctaaat gacaccaatt acgatgagtt taatggctat gctgggagcc361 tcttctcaag tggaccctac gagaaagatg atgaggaagc agatgctatc tatgcagccc421 tggataacag gatggatgaa cagaagacca gacagacggg agcaaaggga gcacaagagc481 aaactagaga aatatcgaat ggaaccgccc aaagtcaaag gtctcaaa<210>25<211>879<212>DNA<213>Homo sapiens<400>251 ggagcaggcg attgaagtaa ttctgtcctg cggggttcag catcatgcac gttccccctg61 ggccattctc tgatgcaggg cacttgctcc acactctggg aacccagatt caccagtctg121 ccctgtcacg tagttgaagg agttgcattt atctttctgg ctcctaattc ctttaaaatt181 acatttgttg cagaacctcc acaaggctaa ataaaattac atttgtgacc atcagagaac
241 tgaataaacc attaactggc catcctggtt tgcagagatc aggttgttga cagttcctgg301 ttgacccaca gctacccatg tcagttatct ccactaacat atccaagaat ctttgtagga361 caatttctcc acctgcaagg tctttcaggt agaactcttc ttttaaggca attagcccat421 tgccaaaagg ttttactggt cttaaagctg tctttctgag atctaattcc aaggacttct481 ccacagctaa gtgagatgcc tcacaccatt aggtgatgct ttggacagaa acagagtatt541 ttcatcttgt gttttacagc aattcctttg gttcggctcc tcaccacttt ctatgccagt601 ctcccaatta tgtccctagt aatggcctat gcgaaaaaaa caaaacaaca aaacaaaaca661 acttttttgg ccgcgatggg ccctgcaaaa gtttttcaga aaccatctcg gtcgggggcg721 ccggagcccc catgaggtac ggcgcaccgc ggcccacacg gggcccaaag gaaacccgcg781 tcgagaacca aacgccgctg gattctcccc caatataaaa ggccggggga caacgccggt841 tttgaacaac ccaggggggc cgcaggcaca ggggccacg<210>26<211>833<212>DNA<213>Homo sapiens<400>261 gtggggccgg gcggccgtgt ggcgcggctg ctcgccccac taatgtggcg cagggcggtt61 tcctcggtgg cggggtccgc ggttggagcc gagcccgggc ttcggctgct ggccgtgcag121 cggcttcccg taggagcagc gttctgccgg gcttgccaga ccccaaactt tgtccgcggc181 ctgcacagcg agcctgggct ggaggagcgg gcggagggga cggtcaacga gggacgccca241 gaatcggacg cggcagatca tactggtccc aagtttgaca tcgatatgat ggtttcactt301 ctgaggcaag aaaatgcaag agacatttgt gtgatccagg ttcctccaga aatgagatat361 acagattact ttgtgattgt tagtggaact tctacccgac acttacatgc catggccttc421 tacgttgtga aaatgtacaa acacctgaaa tgtaaacgtg accctcatgt taagatagaa481 gggaaggaca ctgatgactg gctgtgcgtg gattttggca gcatggtgat tcatttgatg541 cttccagaaa ccagagaaat ctatgaatta gagaaattat ggaccctacg ttcttatgat601 gaccagttag ctcagatagc acctgagaca gtacctgaag acttcattct ttggaataga661 agatgatact tcatctgtga ctccagtgga gttaaaatgt gaactaccat attttatgca721 ctgggttagt caaaaaaaaa aaaaaaacag agaaaaagaa aaaaatgggg gggataatag781 ggggggggga aaaaagggga agaaagggcc tggggcaaag gggtttcggg aca<210>27<211>657<212>DNA<213>Homo sapiens<400>271 aaatgctgag gctgctcaag tttcatccct agtcctattt tctcattcta tatgcacact
61 catttttcat ttaacagact tcactggctt caggcaccat tgttttatta ttcatatttt121 caacccccat atctctctag tgctgtagac ctttatttct acttactagt tatattcacc181 taacatccca tagatatgcc aatgtcaatg tgcctaaaac aaaattgtct ctacctcccg241 ctccttcaaa tctattctgt cttctctaat cctataatta ctctccctca agtcatcatc301 atcacttttc tgggttactg taatagcctt caggctaatt tgctcactgt caatcttgct361 gtctctcatt tcagaatatg ttctgcttgc aggtgacagt aaactagtta aacaaatatt421 taatattagc ttaagcaaaa aaaaaaaaaa aagggggggc cgcccttttt tgtaattttg481 ttttaatgtt atttgtggtt gattagttta ttattgttag tactttcaat atataagagg541 gggggggggc gggatgagag atggggaaga ttaactgtgt tgtattccgc gaaactgtgg601 gtgggggaaa atggaaaacg ccccgtttta ttttgatttt tttttccacc ccatttg<210>28<211>534<212>DNA<213>Homo sapiens<400>281 tttttttttc ctagcagcgt ttattgtcgc ccactctgcc tgacccagtg ttgggcataa61 ggaaaacaga gggaaagagt taaccaccct ggagagagtt ccagcctcaa ggaggagcca121 ggcaggctac agaagtctca cagtgagggc gtggtcctgc aggttgacag aagttagagg181 acagatcagg gaaggctgcc tggaagaggt ggctttgggt tcatccaggc cccctgccca241 cgtgtgcctg ggtctgccca caggacagag gctgatggga aatcctacgt aaagtaccag301 gtcatcggca agaaccacgt ggcagtgccc acacacttct tcaaggtgct gatcctggag361 gcagcaggtg ggcaaattga gctccgcacc tacgtgatgc ccaacgcacc tgtggatgag421 gccatcccac tggagcgctt ccttgtgccc atcgagagca ttgagcgggc ttcggggctg481 ctcttgtgcc caactcctgg cgcgggcagg gcagctcagg ccctcacggc gggc<210>29<211>660<212>DNA<213>Homo sapiens<400>291 ctcaccaaag cccagctaat tttgtatttt tagcagagat ggggtttcac cgtgtggcca61 ggctggtctt gaactcctga cctcaggtga tccaactgcc tcggctccca aagtgctggg121 attacagctg tgagccactg cacccggccg ggaatttatc aaagggggga ctacttagat181 tggggcttca aacctgagtc tagcacaatg cagctggagg ccaggctact cctacaaacc241 caagttctga ctccttccag ggaagtatca gcttaagaaa cttgatcagg aaagtgacac301 tggtctttca aagagccctc tttaccctcc catccctgcc ctccacactg caagctgcat361 tccagccaca acgccctttt ctaaatctat tttcattcat ctcctattct ggtctgtagc
421 cttgattcca accattgaat gcacggagac agcccagtgg cttgtcagat tggtaaataa481 tgacacctgg tggataaatg ttacattcta gaagactatt ctggtcagcc taggtctgaa541 acactaagga gacttggctg agatagggca tccacttgcc tacaggcact ctgcttttat601 tattaaaaat agtcactttg ttactataat aaaatttaac agaaaaaaaa aaaaacaaag<210>30<211>854<212>DNA<213>Homo sapiens<400>301 aggagttata tatttaaaag caattttctg tgttttcttc tttgtaagtt gactcatttg61 tgaagcaatt aggcaaattt tgagaagatc attgttattg tggtttgcag tatatatttc121 ttagtaaata tcacttaaga ttaaattttt cagaaagaaa attatagctt ttttcccaaa181 atatttttaa gatttaatct ttttgtagta tgctacagat ttaattatat taactctttt241 ttaagacatt gaccatgact taacattttg ccttctaaca ccttttaaat ctatgtactt301 taatagttaa gagaaaataa gtttgcagat ttttaataat ctgtttgtaa aaggctatct361 ctaagcctag tatgtgggta aattttacag gtgtgttttt tgataacttt aataattaaa421 aaataagacc tccattttta attttggtgg gcaaaacaaa aaaacaaaaa aacgaaaaca481 acaaacacaa cacaaaacac agaaaacaaa agaaaagaac caacaaagac acaaagccga541 acaaacgaga agaaaaagaa ccaaaaaggg tggggccgag cgcacaaaat gaattactgc601 gaggggggga accaaaaaag atgagacaac aacgaattat attcaaagaa gagagccccc661 agatagagag gaaaaaaaaa agaagaagag agcggcggcg aaaaacaaac aaaaggcgaa721 aggaagaaaa gactcggtga gcgcctggaa ggagaacacc ctgggggaag aaaaaggaga781 acaacacaga atagcggtga gaaaaacaag agttgatgaa acacagttgt cgaaagacgc841 agctaataag atac<210>31<211>519<212>DNA<213>Homo sapiens<400>311 acagcaatag tgcagaatcc agaatggatg tcctctttgt agccatcttt gctgtgccac61 ttatcctggg acaagaatat gaggatgaag aaagactggg agaggatgaa tattatcagg121 tggtctatta ttatacagtc acccccagtt atgatgactt tagtgcagat ttcaccattg181 attactccat atttgagtca gaggacaggc tgaacaggtt ggataaggac ataacagaag241 caatagagac taccattagt cttgaaacag cacgtgcaga ccatccgaag cctgtaactg301 tgaaaccagt aacaacggaa cctagtccag atctgaacga tgccgtgtcc agtttgcgaa361 gtcctattcc cctcctcctg tcgcgtgcct ttgttcaggt ggggatgtat ttcatgtaga
421 aggtggaaga aggctgctat gactctttgg atgggagtct ggcaagagga aattggaaga481 taaaataaat aataagtgaa ataaagaaaa aaaaaaaaa<210>32<211>794<212>DNA<213>Homo sapiens<400>321 ctctgtcgtg gcgcggcttc ccgcggtctt ctctgcaaat gggctccgtg gcctagcgcc61 cccgtccccg ccacccgtga tcgtgcgccg aggcccgcga ggggtcgccg cccagatccc121 accagccagc aagctaaagc atggcggcca tcccctccag cggctcgctc gtggccaccc181 acgactacta ccggcgccgc ctgggttcca cttccagcaa cagctcctgc agcagtaccg241 agtgccccgg ggaagccatt ccccaccccc caggtctccc caaggctgac ccgggtcatt301 ggtgggccag cttctttttc gggaagtcca ccctcccgtt catggccacg gtgttggagt361 ccgcagagca ctcggaacct ccccaggcct ccagcagcat gaccgcctgt ggcctggctc421 gggacgcccc gaggaagcag cccggcggtc agtccagcac agccagcgct gggcccccgt481 cctgacctga gcggttacca ccagccccag gcctgcggag gcgctagtcc accagagccc541 ctccccgccc ctctccccac tccgcatccc tcgcccccct ccccacctcc caccccccac601 cctgtaaact aggcggctgc agcaagcaga ccttcgcatc aacacagcag acaccaaaaa661 ccagtgagag ccccgctctc taccgcccgg ccccagcact cgctagcttt cctgacacct721 ggaactgtgc acctggcacc aagcggaaaa taaactccaa gcagccagta gccccgaaaa781 aaaaaaaaaa aaaa<210>33<211>632<212>DNA<213>Homo sapiens<400>331 gacggtcgcc gcgttccatc gtcgcgcggc ccttcgggcg cccgagcccg caatgtcggg61 ccccaacgga gacctgggga tgccggtgga ggcgggagcg gaaggcgagg aggacggctt121 cggggaagca gaatacgctg ccatcaactc catgctggac cagatcaact cctgtctgga181 ccacctggag gagaagaatg accacctcca cgcccgcctc caggagctgc tggagtccaa241 ccggcagaca cgcctggagt tccagcagca gctcggggag gcccccagtg atgccagccc301 ctaggctcca agagccccca accgggaccc aaccctgcct ccctgggcta ggctctggcc361 tgggcactca ccccctggct tagacacctt ctcaagggct ggccttcagg gacccctggt421 gggtctgcct gcctgggcca cccttcctgc ctgggcctcc ccttggccta cctgggccag481 cccccaccac ctggcatgcc ctcctggggc caagagtggg cctgcaaccc acccacttgc541 ctgcccaccc aactcctggg cgctccccac tctgcccagg ccttgagtgt ccacattaaa
601 tgggtctccc acaaaaaaaa aaaaaaaaaa aa<210>34<211>876<212>DNA<213>Homo sapiens<400>341 gcgcggcggc tcccggcagg agggagaggg cacaccgcca gccccaggcc aggctgcgag61 ggccgcggac ccgagctggg aaggaccttg ggcggacgag ccgcgcgtcc cgcagccatg121 gagcaggatg acccggtcga ggcgctgacg gagctgcgcg agcggcggct gggcgcgctg181 gagctgctgc aggcggcggc cggctcgggc ttggcagcct acgcggtgtg ggcgctgctg241 ctccagcccg gcttccggcg cgtgccgctg cggctgcagg tgccctacgt cggcgcgagc301 gcgcggcagg tggagcacgt gttgtcgctg ctgcgaggac gccccggaaa aacggtggat361 ctgggctctg gcgacggcag gatcgtgctg gcggcccaca ggtgcggcct ccgcccggcc421 gtgggctacg agctgaaccc ctggctggtg gcgctggcgc ggctgcacgc ctggagggcc481 ggctgtgccg gcagcgtctg ctatcgccgc aaggatctct ggaaggtgag cctgagggac541 tgccgcaacg tgtctgtgtt cctggcccct agcgtgctcc cgctgctgga ggacaagctg601 cggacagagc tgcctgctgg ggcccgcgtg gtgtctgggc gcttcccact ccccacctgg661 cagcctgtga ccgcggttgg cgagggcctg gaccgagtat gggcttatga tgttcctgag721 ggtgggcagg ctggggaggc cgcctcctcg cggataccca tccaggctgc ccccggacct781 agttctgccc ccatcccggg gggccttatt tctcaggcca gctgagtatt agacacgata841 aagactctgt gggttctaaa aaaaaaaaaa aaaaaa<210>35<211>1075<212>DNA<213>Homo sapiens<400>351 cagagctgct gtcatggcgg ccgctctgtg gggcttcttt cccgtcctgc tgctgctgct61 gctatcgggg gatgtccaga gctcggaggt gcccggggct gctgctgagg gatcgggagg121 gagtggggtc ggcataggag atcgcttcaa gattgagggg cgtgcagttg ttccaggggt181 gaagcctcag gactggatct cggcggcccg agtgctggta gacggagaag agcacgtcgg241 tttccttaag acagatggga gttttgtggt tcatgatata ccttctggat cttatgtagt301 ggaagttgta tctccagctt acagatttga tcccgttcga gtggatatca cttcgaaagg361 aaaaatgaga gcaagatatg tgaattacat caaaacatca gaggttgtca gactgcccta421 tcctctccaa atgaaatctt caggtccacc ttcttacttt attaaaaggg aatcgtgggg481 ctggacagac tttctaatga acccaatggt tatgatgatg gttcttcctt tattgatatt541 tgtgcttctg cctaaagtgg tcaacacaag tgatcctgac atgagacggg aaatggagca
601 gtcaatgaat atgctgaatt ccaaccatga gttgcctgat gtttctgagt tcatgacaag661 actcttctct tcaaaatcat ctggcaaatc tagcagcggc agcagtaaaa caggcaaaag721 tggggctggc aaaaggaggt agtcaggccg tccagagctg gcatttgcac aaacacggca781 acactgggtg gcatccaagt cttggaaaac cgtgtgaagc aactactata aacttgagtc841 atcccgacgt tgatctctta caactgtgta tgttaacttt ttagcacatg ttttgtactt901 ggtacacgag aaaacccagc tttcatcttt tgtctgtatg aggtcaatat tgatgtcact961 gaattaatta cagtgtccta tagaaaatgc cattaataaa ttatatgaac tactatacat1021 tatgtatatt aattaaaaca tcttaatcca gaaaaaaaaa aaaaaaaaaa aaaaa<210>36<211>726<212>DNA<213>Homo sapiens<400>361 ctcccaagtc ctggaacggg cctccccacc ctccggggca ccgacactca cggagtcact61 ccaacttctc tgggtgctcc gtggagcgaa gagcgctcta ggacaagcaa ggattcctct121 tctcctgtaa atgaccacag aggaagacaa tgaatgaggt gaaagaatcc cttcgcagca181 tcgagcagaa gtacaagctc ttccagcagc agcagctcac cttcaccgcc gctctggagc241 actgcaggga gaacgcccac gacaagatcc ggcccatctc cagcattgga caggtgcaga301 gctacatgga acactactgc aacagctcca cagaccggcg ggttctgctc atgttcctgg361 acatctgttc agagctgaat aagctctgcc agcactttga ggccgtgcac tctggcaccc421 cagtcaccaa caacctcctg gagaaatgca aaaccctcgt tagccaaagc aacgacttaa481 gcagcctcag agcaaaatac cctcatgatg tggtgaacca cctcagctgt gacgaggccc541 ggaaccacta cggcggcgtg gtcagcctca tccccctcat cctagactta atgaaagaat601 ggatcgccca ctccgagaag ttgccgcgca aggtgctgca gcacgggacg acttagcttc661 tgtattattg ttgcttcctc agggggagac agtcaagaat aaaaagtatt ctaccaccta721 aaaaaa<210>37<211>925<212>DNA<213>Homo sapiens<400>371 gcgcggtagc atcgcggagt cggtgcttta gtacgccgct ggcaccttta ctctcgccgg61 ccgcgcgaac ccgtttgagc tcggtatcct agtgcacacg ccttgcaagc gacggcgcca121 tgagtctgac ttccagttcc agcgtacgag ttgaatggat cgcagcagtt accattgctg181 ctgggacagc tgcaattggt tatctagctt acaaaagatt ttatgttaaa gatcatcgaa241 ataaagctat gataaacctt cacatccaga aagacaaccc caagatagta catgcttttg
301 acatggagga tttgggagat aaagctgtgt actgccgttg ttggaggtcc aaaaagttcc361 cattctgtga tggggctcac acaaaacata acgaagagac tggagacaat gtgggccctc421 tgatcatcaa gaaaaaagaa acttaaatgg acacttttga tgctgcaaat cagcttgtcg481 tgaagttacc tgattgttta attagaatga ctaccacctc tgtctgattc accttcgctg541 gattctaaat gtggtatatt gcaaactgca gctttcacat ttatggcatt tgtcttgttg601 aaacatcgtg gtgcacattt gtttaaacaa aaaaaaaaaa aaaaaaggaa aaaccaacct661 catggcctgt gggttatttt ggtcttgtaa ggatccattt ctttaaaata ctgacatata721 gagttgtacc ttatatagaa tatagttgta tcttgaagtc aacatattaa attattctca781 aaattatgta tttgcagatt gtacttgtaa gtttcaaaga aaaattacca tcttttcata841 ttgacctgga aactaaatag gatgtgattc agctacatta atttcttaat acaatctaaa901 aaaaaaaaaa aaaaaaaaaa aaaaa<210>38<211>711<212>DNA<213>Homo sapiens<400>381 cttttttttt tttttgcgaa gtgggctcgt gggttggcag tatgagagtt gtaatggccc61 gactgttgag tgagggggag caggggatcc caacggcttg cgctgccttt gcgcagcagc121 cggcgggcga gccacgtcgc ggcctggctg gggtaggaga gggcggtccc cagtgcagtt181 gggtgaacta ccgttgcaca ctggagtttc tggtgtcttt gcttggaact gacctagctc241 gtggcagggg gaactcggct agcggcccca cagcccctgc tgactcaaaa caactaatga301 actctggaag cccagccagg gacaatgcac cttcacagag attctgcact aatctgagtg361 aaggtctaag gtttggaatc tccccctcat ggagagaagc tttgtatggc tgtcatgctt421 agacagtgat tcctgcaact tgaccttcag gctgggagag gtggagagcc atgcctgttc481 tccttccttg ctatggaatt tgctgacaca atatcttccg cctggtgctg ggcatatcct541 aagaacttac aactttcctg tattatcctg tgtgagcagc tgtcacctta ttgggggaaa601 aatgcctgaa aattaggggg cacttcaagt agatagcttc tatttcctat atttgtctta661 tatacaagta tttgctttta tcaaaataat tccaataaag cattttaaag t<210>39<211>1105<212>DNA<213>Homo sapiens<400>391 gcggccaccg ttttaaccac gattggcgaa gcgccgtcat ttcggagcga ctcagcgcct61 gcccgccctc tcgccgcgtc gccggtgcct gcgcctcccg ctccacctcg cttcttctct121 cccggccgag gcccggggga ccagagcgag aagcggggac catgttccga cgcaagttga
181 cggctctcga ctaccacaac cccgccggct tcaactgcaa agatgaaaca gaatttagaa241 acttcatcgt ttggcttgaa gaccagaaaa tcaggcacta caagattgaa gacagaggga301 atttaagaaa catccacagc agcgactggc ccaagttctt tgaaaagtat ctcagagatg361 ttaactgtcc tttcaagatt caagatcgac aagaagctat tgactggctt cttggtttag421 ctgttagact tgaatatgga gataatgctg aaaaatacaa ggatttagta cctgataatt481 caaaaactgc tgacaatgca actaaaaatg cagaaccatt gatcaatttg gatgtaaata541 atcctgattt taaggctggt gtgatggctt tggctaacct gcttcagatt cagcgtcatg601 atgattacct ggtaatgctt aaggcaattc ggattttggt tcaggagcgc ctgacacagg661 atgcagttgc taaggcaaat caaacaaaag agggcttacc tgttgcttta gacaaacata721 ttcttggttt tgacacagga gatgcagttc ttaatgaagc tgctcaaatt ctgcgattgc781 tgcacataga ggagctcaga gagctacaga caaaaatcaa cgaagccata gtagctgttc841 aggcaattat tgctgatcca aagacagacc acagactggg aaaagttgga agatgaacac901 ttgaggactt cagcttctca cctacttagt acagttggga accatacact tctggcatgt961 ttggaaatca aaatgtcaca ttctcggggg aggaagccca gaaaattggg tatgttctag1021 agatttacca ccattgctta ttgctttttt ctttaataaa gtttaggaaa gtagaaaaaa1081 agtgagttgg aaaaaaaagt cgaaa<210>40<211>418<212>DNA<213>Homo sapiens<400>401 gcctgtcgac gtgttcttcc ggtggcggac ggcggattag ccttcgcggg gcaaaatgga61 gctcgaggcc atgagcagat ataccagccc agtgaaccca gctgtcttcc cccatctgac121 cgtggtgctt ttggccattg gcatgttctt caccgcctgg ttcttcgttt acgaggtcac181 ctctaccaag tacactcgtg atatctataa agagctcctc atctccttag tggcctcact241 cttcatgggc tttggagtcc tcttcctgct gctctgggtt ggcatctacg tgtgagcacc301 caagggtaac aaccagatgg cttcactgaa acctgctttt gtaaattact tttttttact361 gttgctggaa gtgtcccacc tgctgctcat aataaatgca gatgtataca aaaaaaaa<210>41<211>725<212>DNA<213>Homo sapiens<400>411 gctccgggtg tcccgagagt tgtcctgcgc cggtgttccc acgtgcggcc tgaacctgag61 cgcataatgt tatgaggaga tgggagcact agtgattcgc ggtatcagga atttcaacct121 agagaaccga gcggaacggg aaatcagcaa gatgaagccc tctgtcgctc ccagacaccc
181 ctctaccaac agcctcctgc gagagcagat tagtctctat ccagaagtta aaggagagat241 tgctcgtaaa gatgaaaagc tgctgtcgtt tctaaaagat gtgtatgttg attccaaaga301 tcctgtgtct tccttgcagg taaaagctgc tgaaacatgt caagagccga aggaattcag361 attgccgaaa gaccatcatt ttgatatgat aaatattaag agcattccca aaggcaaaat421 ttccattgta gaagcattga cacttctcaa taatcataag cttttcccag aaacctggac481 tgctgagaaa ataatgcaag aataccagtt agaacagaaa gatgtgaatt ctcttcttaa541 atattttgtt acttttgaag tcgaaatctt ccctcctgaa gacaagaaag caatacgatc601 aaaatgaaga aaatcacaga aatttcctat gtgtactcct catccctcct gctgtatatt661 ttctcatttt ttgcgtaata aattatgtta attaccaaaa aaaaaaaaaa aaaaaaaaaa721 aaaaa<210>42<211>672<212>DNA<213>Homo sapiens<400>421 caggccagcc ctggggcgcc ttaaaaaccg gagctggcgc ttggcatcgc cactctgggc61 aggatccaac gtcgctccag ctgctcttga cgactccaca gataccccga agccatggca121 agcaagggct tgcaggacct gaagcaacag gtggagggga ccgcccagga agccgtgtca181 gcggccggag cggcagctca gcaagtggtg gaccaggcca cagaggcggg gcagaaagcc241 atggaccagc tggccaagac cacccaggaa accatcgaca agactgctaa ccaggcctct301 gacaccttct ctgggattgg gaaaaaattc ggcctcctga aatgacagca gggagacttg361 ggtcggcctc ctgaaatgac agcagggaga cttgggtgac cccccttcca ggcgccatct421 agcacagcct ggccctgatc tccgggcagc caccacctcc tcggtctgcc ccctcattaa481 aattcacgtt cccaccctgt gtccacttca tgattcctcg caagctgggc ccagtcctct541 catcccaaga gcagagccac cgtagccgga gtcctagcct cccaaattcg gaaatccaat601 ccaacggtct caggaatgtt ttccatcccg ccacgcgcct cccgaagctc ccagaccgga661 ggctcagccc cc<210>43<211>469<212>DNA<213>Homo sapiens<400>431 tcaaattaat gtccatccaa atgaatattt tactataaaa taaaagaagt aaataataca61 gagtaattat ttattactta ttcatttcta ggcaggatga gggtctgaga gtataataca121 gttcaaataa agaagttacc tggcttaaaa atagctactt gctaaagata gaaaattatt181 ataaatctta aaaacaaaca ggcaaaacat cttgaaagtt ttataggctt tgcattttaa
241 aattatagat gattagaaac tatacttttc ttctacactg atactaaaat agtttcattt301 tcagcttaaa ttacaacagt taataattta tctgactgta tataaacata atattctttc361 tacattttag tttaaaatag tacaatatta aaaagcttat aaattggcca ataaacatgg421 gaactcacac atacatttcn ggacattggg ggggattatt ataattatt<210>44<211>411<212>DNA<213>Homo sapiens<400>441 tttttttttt tttttttttt ttcctcattt aagtcttgat tccttcccca attttattgc61 caaataaact ggtctcttgc tacaggtatg taactggttg aactaataaa tgtgaatcac121 tggaaggcaa tctgatacaa gttattcttg attacaatca atcttttaag tatgcttaag181 ggtcctaaca tccagcagcc ttggagttaa tgatctttaa tgatttctcc catttatact241 gtgtttttaa caagcataat aaaggaaaat ataccagagg tttattaagt atgacaaact301 gtactttaaa taaatngttg ctattgactt taggacttct tggcacaaag tttatagatt361 agcaatacta atacccttaa aaatggcctt ttgaaggggt aattcctaca c<210>45<211>618<212>DNA<213>Homo sapiens<400>451 gtcttatgtc tctaatctgc actgttcagc tcttttaggc actgcaaagt tgtcttgaat61 taggaaagag gtgctagaat gtgggcgtgg gtgttgacct acatctgaac aatttacata121 tgattcacca caattaaaca atttggtttg aaatagctat aattaagtta ttatcagaga181 agtatttact agtctagaaa ttctaaattt atcttcacat acaccctaac tgagaaaagg241 gccacatttt ctgcactcta ttaagtaaag caaatgctga actaaatgcc tccatgttaa301 catttatatt gttaagttac tgacagcata ttctatgaat gattacgtta gtcgtttctt361 taaaaattat aggtttgaaa tagcaagaaa aatatgaaat gatggtagac aaaaaagagt421 ttcagtttcn aacttctaac tatatatata cacacacaca tgcacacaga attgccttcc481 cggatgtata gaaattatat acagccatgt ccaggcncga tggaaattat gggggaatat541 ccaanttagg atacncgtgc cgaatcgccg ggtntaaata atacnggttt ataatggacn601 atccacaatc ctggttta<210>46
<211>577<212>DNA<213>Homo sapiens<400>461 gtaatgttaa ttaactttat tcacattttt ccaagtaaat acaatattaa ctataagcta61 aaaacaatac atagttctta gaaaatacaa ccccgaatta ttgtgtggcc tgggctttgt121 gattatgtgc ccagcctttg ttcctccaga attttaaagt gaatgcagtg ctcaaaacaa181 acttacgata gcacagggaa accttcccta aataaagttt tgttattaga agagaaatcc241 aaaaagaggc ctctactgcc ttcattcaag tgtcgagaat ttcnaaatgg gaaataatat301 taaaagagca atcgattttt ttccatctca cactagtagg cttgaaggag gcaaatttgg361 gaccaaaaat ctagacatag ctccttgcat agggtttgat attaagcaca cacttggcac421 tggaataact ccatatccac cccnatagta ccaaaattca tttcaaatgg caaacgttgt481 gcnatggacc agggtcatat ttttaaagat cactccctta aaatatccat ttttactgng541 tcaccaaatt atataataag ggntcttata tgggggt<210>47<211>464<212>DNA<213>Homo sapiens<400>471 aatagactcc cgatttggtg aaacttttat tcatgttgca acgtttttaa aatttgtttt61 catttttctt tgacccatat cattgttata ctagagctta acttcttatc tcaaaatata121 attccttatt tttcctgttc taggaactat ttggctaaat tatttttctt catagtcatt181 gggattataa gtacaaggat gctttctaca catgtatgtt tcagcttaaa atgaatagcc241 cttgagcatg ttgcatgatg tctgccttct ccgtcactct gtaatttctc tctttttctc301 tctctcatct tctgtatctg tagtctcaac tacccagaag gctgaggcag gaggattagt361 tgagtccagg agatcgaggc tgcagtgagc tgtgattgca ccactgtact ccagcctagg421 tggacagaat aagactccct tggttctaga gacctttcaa agga<210>48<211>421<212>DNA<213>Homo sapiens<400>481 cattttaaaa taaacattta attcaacatt gcaacaggag ttgaactgta tttaaaacag61 aagcatatta atgactaaaa aaatgtgtat attaatcttt agttcccttt tgaggggatt121 ttgattgcca gttctccgca gtgaaatgct aggaaaatgt ctttagaaga cagaattctt
181 tctgaatgca agtctaaaat gcttaaaaga gattaaaggt tttaaagacc caaatcagaa241 atatccatta gatacatctc atttgaatcc taagagttta aacataataa acgctgtatt301 tatttatttt atttcttttt tatttttttg aggcagagtc tcactttgtt gcccaggctg361 gagtgcagtg gtgcagtctt ggctcactgc agcctgtctt ctgggttaaa acgattctcc421 t<210>49<211>444<212>DNA<213>Homo sapiens<400>491 ntttggtttt gggacatata agtttaatta cctgctagat tatcagatgg aaatatgtgg61 taaatggatg gaaacaaggt acataaactt aggaggcaga aatttttaaa ctttagggtt121 agacattcac cagattggac aatttttaac atttaaaata aaactttttg tcataaaaaa181 caaatgtatt aaaactagtt tccagaactg cccaaatgac tttttaaaca tgacttaaat241 gtcgttttga caaatctatc caaaatacta attttccttt taggaactta gtttataatt301 tatatattaa agtgccctta attngatatt catcgggata ccttcctctg agccctaatg361 gttttgggga ggcctaggcg gtggatcacc tgagggtcag gagttcgaga ccagcctggg421 ccaacgtggg tggaaaccca gtct<210>50<211>688<212>DNA<213>Homo sapiens<400>501 ttcggcacga ggaacctttt ttcacctcgt ctgaaatggc tgcctcccag tgtctctgct61 gctcaaaatt tctcttccag agacagaacc tcgcctgttt cctcacaaac ccacactgtg121 gcagccttgt taatgcagat ggccatggtg aagcgtggac agattggaat aatatgtcca181 agtttttcca gtatggatgg cgatgcacca ctaatgagaa tacctattca aaccgtaccc241 tgatgggcaa ctggaaccag gaaagatatg acctgaggaa tatcgtgcag cccaaaccct301 tgccttccca gtttggacac tactttgaaa caacatatga tacaagctac aacaacaaaa361 tgccactttc aacacataga tttaagcgag agcctcactg gttcccagga catcaacctg421 aactggatcc tccccgatac aaatgcacag aaaagtcaac ttacatgaat agctattcaa481 agccttaaat tgggcatcac tcaggatgtg tataagatct taatattgac tagtttcaca541 tccaggtttc taagaaatga taagatactt cacttttcca gagtgaaatg taggagggag601 cacattctaa gtacagctaa aaatttagct cactgtaaca cagtttcact ctctgaataa661 ataaagcaaa aaacacagta aaaaaaaa
權(quán)利要求
1.一類人類肝臟表達序列標簽的序列，其包括(a)SEQ ID No.1～SEQ ID No.50所示的序列；(b)SEQ ID No.1～SEQ ID No.50所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.50所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數(shù)條的組合。
2.根據(jù)權(quán)利要求1所述的一類人類肝臟表達序列標簽的序列，其特征在于所述序列包括具有SEQ ID No.1～SEQ ID No.50所示的序列。
3.一種探針分子，其特征在于所述的探針分子含有權(quán)利要求1中所述的序列中約8-100個連續(xù)的核苷酸。
全文摘要
本發(fā)明公開了一類人類肝臟表達序列標簽。利用本發(fā)明的在人類肝臟中表達的表達序列標簽，可以方便的尋找出在人類肝臟中表達的相關(guān)基因，從而在研究肝臟疾病的致病機理以及開發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
文檔編號C07H21/04GK1955290SQ20051003082
公開日2007年5月2日 申請日期2005年10月28日 優(yōu)先權(quán)日2005年10月28日
發(fā)明者黃健, 韓澤廣 申請人:上海人類基因組研究中心