專利名稱：評(píng)估結(jié)腸直腸癌的制作方法
背景本申請(qǐng)要求2002年3月29日申請(qǐng)的序列號(hào)為60/368,798的美國臨時(shí)申請(qǐng)的利益。
本發(fā)明涉及基于生物樣本基因表達(dá)分布圖的結(jié)腸直腸癌的診斷和預(yù)測。
結(jié)腸直腸癌是一種異源疾病，由據(jù)認(rèn)為是通過三種主要分子機(jī)制形成的腫瘤組成1)與染色體不穩(wěn)定性相結(jié)合的多發(fā)性結(jié)腸腺癌(APC)基因，或β-連環(huán)蛋白基因的突變，2)與微衛(wèi)星序列不穩(wěn)定性相關(guān)的DNA錯(cuò)配修復(fù)基因如MLH1，MSH2，PMS2和MSH6的突變，和包含短重復(fù)序列基因的突變，以及3)由腫瘤抑制基因啟動(dòng)子區(qū)的過甲基化誘導(dǎo)的基因沉默。結(jié)腸直腸癌個(gè)體的遺傳互補(bǔ)可能包括遺傳不穩(wěn)定性、特異突變和基因沉默的不同結(jié)合。染色體不穩(wěn)定性(CIN)一般是癌癥的普遍特征。它意味著其中所有的或大部分的染色體丟失或增加了的非整倍體表型。伴隨短重復(fù)序列突變率的增加，在二倍體腫瘤中發(fā)現(xiàn)了微粒體不穩(wěn)定性(MIN)。這兩種遺傳不穩(wěn)定性的形式在結(jié)腸直腸癌中很普遍。
因此結(jié)腸直腸癌具有復(fù)雜的起源并且包括不同生物通路間大量的相互作用。用于協(xié)助提供診斷、預(yù)測或治療監(jiān)測結(jié)果的血清標(biāo)記、組織學(xué)和細(xì)胞學(xué)檢查通常不具有所期望的可信度。同時(shí)，使用單獨(dú)的遺傳標(biāo)記(例如一特殊基因的表達(dá)增加)可能是有益的，癌癥的多樣性使遺傳標(biāo)記的組合可能成為最好的途徑。
發(fā)明概述本發(fā)明是評(píng)估結(jié)腸直腸癌存在或不存在，或者被認(rèn)為患有結(jié)腸直腸癌的患者其可能狀態(tài)的方法。在本方法中，通過分析患者樣本的基因表達(dá)分布圖確定患者是否得了結(jié)腸直腸癌，患者是否未得結(jié)腸直腸癌，患者是否可能要得結(jié)腸直腸癌，或正在接受治療的結(jié)腸直腸癌患者對(duì)治療的反應(yīng)。
本發(fā)明的一個(gè)方面涉及用于實(shí)施該方法的制品。這樣的制品包括基因表達(dá)分布圖或固定于機(jī)器可讀媒介如計(jì)算機(jī)可讀媒介的基因表達(dá)分布圖的表現(xiàn)物。
用來鑒別基因表達(dá)分布圖的制品也可以包括用于捕捉和/或顯示基因存在，缺失或表達(dá)程度的基片或平面，如微陣列。
發(fā)明詳述很少發(fā)現(xiàn)組織樣本中單純的特定核苷酸序列的存在或缺失具有診斷或預(yù)測價(jià)值。另一方面，關(guān)于多種蛋白、肽或mRNA表達(dá)的信息正越來越受到重視。具有表達(dá)蛋白、肽或mRNA(這樣的序列是指“基因”)的潛在可能的特定核苷酸序列僅在給定細(xì)胞的基因組中存在并不能決定該蛋白、肽或mRNA一定會(huì)在給定的細(xì)胞中表達(dá)。給定的基因能否表達(dá)蛋白、肽或mRNA以及表達(dá)到何種程度取決于多種復(fù)合因子。不考慮理解和評(píng)估這些因子的困難程度，檢測基因表達(dá)可以為如腫瘤發(fā)生、轉(zhuǎn)移、細(xì)胞凋亡以及其他臨床有關(guān)現(xiàn)象等重要事件的發(fā)生提供有用的信息。通過基因表達(dá)分布圖可以發(fā)現(xiàn)基因激活或失活程度的相對(duì)指標(biāo)。本發(fā)明的基因表達(dá)分布圖可用于結(jié)腸直腸癌患者的診斷和治療。
樣品制備需要收集患者樣本。本發(fā)明使用的病例樣本是那些疑為含有患病細(xì)胞的樣本，如取自結(jié)腸樣本或外科切除樣本的上皮細(xì)胞。獲得可疑樣本的一種有用技術(shù)是激光捕捉微切片技術(shù)(LCM)。激光捕捉微切片技術(shù)提供了一種選擇需研究細(xì)胞的方法，并最小化由細(xì)胞種類的不均一性造成的易變性。因此，正常細(xì)胞和癌細(xì)胞之間的基因表達(dá)中度或細(xì)小的變化都可以被輕易檢測到。在優(yōu)選的方法中，樣本包括從外周血中提取的循環(huán)系統(tǒng)上皮細(xì)胞。這可以按照多種方法獲得，但最優(yōu)化的方法是參考引用Immunivest公司的美國專利6,136,182所描述的磁性分離技術(shù)。一旦得到含有所關(guān)注細(xì)胞的樣本，就提取其RNA，擴(kuò)增得，并獲得基因表達(dá)分布圖，優(yōu)選的通過微陣列獲得合適組配的基因。
確證基因表達(dá)分布的優(yōu)選方法包括，確定一個(gè)能夠編碼蛋白質(zhì)或肽的基因所產(chǎn)生的RNA的量。這可通過逆轉(zhuǎn)錄酶PCR(RT-PCR)，競爭性RT-PCR，實(shí)時(shí)RT-PCR，差異顯示RT-PCR，Northern雜交分析和其他相關(guān)實(shí)驗(yàn)實(shí)現(xiàn)。當(dāng)可能用單個(gè)PCR反應(yīng)實(shí)施這些技術(shù)時(shí)，最好擴(kuò)增來自mRNA的互補(bǔ)DNA(cDNA)或互補(bǔ)RNA(cRNA)并用微陣列對(duì)其分析。對(duì)本領(lǐng)域的技術(shù)人員來說大量不同的陣列構(gòu)型及其生產(chǎn)方法是已知的，并且也在如下的美國專利中有描述5,445,934；5,532,128；5,556,752；5,242,974；5,384,261；5,405,783；5,412,087；5,424,186；5,429,807；5,436,327；5,472,672；5,527,681；5,529,756；5,545,531；5,554,501；5,561,071；5,571,639；5,593,839；5,599,695；5,624,711；5,658,734；和5,700,637；這些專利披露的技術(shù)在此引入作為參考。
微陣列技術(shù)允許同時(shí)檢測數(shù)千基因的穩(wěn)定狀態(tài)的mRNA水平，因此它為檢測非控制的細(xì)胞增殖效果，如啟動(dòng)、抑制或調(diào)節(jié)等提供了一個(gè)強(qiáng)有力的工具?，F(xiàn)在兩種微陣列技術(shù)正被廣泛使用。第一種是cDNA陣列，第二種是寡核苷酸陣列。盡管這些芯片在構(gòu)造上存在差異，但其下游數(shù)據(jù)的分析和輸出實(shí)質(zhì)上是相同的。這些分析的結(jié)果典型地是對(duì)由標(biāo)記探針獲得的信號(hào)強(qiáng)度的測定，所述的探針用于檢測與陣列上已知位點(diǎn)的序列雜交的、來自樣品的cDNA序列。典型地，所述的信號(hào)強(qiáng)度與cDNA的含量，或者說樣本細(xì)胞中所表達(dá)的mRNA的量成比例。有許多這樣的技術(shù)可以獲得并且是有用的。優(yōu)選的檢測基因表達(dá)的方法可以在美國專利中找到，如Linsley等人的專利6,271002；Friend等人的專利6,218,122；Peck等人的專利6,218,114；Wang等人的專利6,004,755，其所公開的內(nèi)容均在此引入作為參考。
基因表達(dá)水平的分析通過比較強(qiáng)度進(jìn)行。最好是通過制備一種待測樣品中基因表達(dá)強(qiáng)度相對(duì)于對(duì)照樣品中基因表達(dá)強(qiáng)度的比值矩陣進(jìn)行。例如，可以將疾病組織中基因表達(dá)的強(qiáng)度與相同類型正常組織中的表達(dá)強(qiáng)度進(jìn)行比較(例如患病的結(jié)腸組織樣本對(duì)正常的結(jié)腸組織樣本)。這個(gè)表達(dá)強(qiáng)度的比值顯示出被測樣本和對(duì)照樣本中基因表達(dá)的倍數(shù)變化。
基因表達(dá)分布圖也可以通過很多方法顯示。最普遍的方法是用樹突狀圖處理原始的熒光強(qiáng)度或矩陣系數(shù)，其中列表示被測樣品，行表示基因。數(shù)據(jù)經(jīng)這樣的處理后那些具有相似表達(dá)分布的基因就會(huì)相互鄰近。每個(gè)基因的表達(dá)比率可用一種顏色顯示。例如，小于一的比率(表示下調(diào))可以出現(xiàn)在圖譜的藍(lán)色部分，而大于一的比率(表示上調(diào))可以出現(xiàn)在圖譜的紅色部分。商業(yè)上可購買到的計(jì)算機(jī)軟件程序很適合顯示這樣的數(shù)據(jù)，包括Silicon Genetics有限公司的“GENESPRING”，Partek有限公司的“DISCOVERY”和“INFER”軟件。
用于本發(fā)明的方法的受調(diào)節(jié)基因如表1所示。患病細(xì)胞中差異表達(dá)的基因表現(xiàn)為上調(diào)或下調(diào)。上調(diào)和下調(diào)是相對(duì)的術(shù)語，表示可檢測到的相對(duì)于某種基線的基因表達(dá)的量的差異(超出檢測系統(tǒng)的噪音影響)。在這種情況中，基線是正常細(xì)胞測得的基因表達(dá)。再使用同樣的測量方法確定患病細(xì)胞中所關(guān)注的基因相對(duì)于基線水平是上調(diào)或下調(diào)。上下文中，患病的，是指伴隨細(xì)胞非控制增殖而出現(xiàn)的機(jī)體狀態(tài)的改變，此種改變中斷。擾亂，或潛在的打亂機(jī)體功能的適當(dāng)表現(xiàn)。當(dāng)某人基因型或表型的一些方面一致表現(xiàn)出存在特定疾病時(shí)，他就被診斷為患有該種疾病。作出診斷或預(yù)測的行為包括確定疾病表象/狀態(tài)的情況，如治療監(jiān)測。在治療監(jiān)測中，比較經(jīng)過一段時(shí)間治療后的基因表達(dá)確定基因表達(dá)分布圖是否有變化，或其變化與正常組織的模式更為一致來考慮療程的效果從而作出臨床判定。
優(yōu)選的，上升或下降調(diào)節(jié)的水平是以雜交的微陣列探針測量強(qiáng)度的成倍變化來區(qū)別的。2倍差異或p值小于0.5對(duì)于作出這樣的辨別是優(yōu)選的。即當(dāng)認(rèn)為與在正常細(xì)胞中相比一個(gè)基因在患病細(xì)胞中差異表達(dá)時(shí)，患病細(xì)胞中的強(qiáng)度至少是超出正常細(xì)胞的2倍或低于正常細(xì)胞2倍。差異倍數(shù)越大，基因越優(yōu)選用作診斷。本發(fā)明選擇用作分析表達(dá)分布圖的基因具有產(chǎn)生使該基因區(qū)別于正?；蚧蚍钦{(diào)節(jié)基因的信號(hào)的表達(dá)水平，該信號(hào)量超出使用臨床實(shí)驗(yàn)室檢測設(shè)備的背景量。
利用統(tǒng)計(jì)值可用于確定地使調(diào)節(jié)基因與非調(diào)節(jié)基因和噪音區(qū)別開。通過統(tǒng)計(jì)檢驗(yàn)發(fā)現(xiàn)在不同樣本組之間有顯著差異的基因。Student’s T檢驗(yàn)就是一個(gè)發(fā)現(xiàn)兩組之間顯著差異的有力的統(tǒng)計(jì)檢驗(yàn)的例子。p值越小，不同組之間基因顯示差異的證據(jù)就越有說服力。然而既然微陣列可以同時(shí)檢測多個(gè)基因，那么就需要同時(shí)進(jìn)行萬次的統(tǒng)計(jì)檢驗(yàn)。因此，就存在偶爾看到極小p值的可能，并利用Sidak校正以及作出隨機(jī)化/排列實(shí)驗(yàn)進(jìn)行調(diào)整。T檢驗(yàn)中p值小于.05就證明基因是差異顯著的。更有說服力的證據(jù)是加入Sidak校正因子后p值小于.05。對(duì)于每組中的大量樣品，經(jīng)過隨機(jī)化/排列檢驗(yàn)后p值小于.05是差異顯著的最有說服力的證據(jù)。
另一個(gè)可用來選擇產(chǎn)生強(qiáng)于非調(diào)節(jié)基因和噪音信號(hào)的基因的參數(shù)是絕對(duì)信號(hào)差異測量值。優(yōu)選的，調(diào)節(jié)基因的表達(dá)形成的信號(hào)與正?；蚧蚍钦{(diào)節(jié)基因(以絕對(duì)值為基礎(chǔ))至少有20％的差異。更優(yōu)選的，調(diào)節(jié)基因形成的表達(dá)模式與正?；蚧蚍钦{(diào)節(jié)基因至少有30％的差異。
將基因分組以便于通過獲得一組基因中一套基因的信息為診斷、預(yù)測或治療等相關(guān)臨床判斷提供可靠的基礎(chǔ)。這些套基因構(gòu)成了本發(fā)明的框架(portfolios)。此例中，由該框架支持的判斷包括結(jié)腸直腸癌。基因表達(dá)分布圖的框架包含實(shí)施例3中描述的基因聯(lián)合組合。對(duì)于大多數(shù)診斷標(biāo)記物來說，最好使用盡量少的標(biāo)記物就足以充分作出一個(gè)正確的醫(yī)學(xué)判斷。這就避免了使用那些懸而未決需作進(jìn)一步分析的治療措施帶來的延誤，同時(shí)又避免了時(shí)間和資源的不合理使用。此例中，這樣的最小量的框架包含實(shí)施例4中的基因組合。
優(yōu)選的，構(gòu)建這樣的框架以保證該框架中的基因組合相對(duì)于單個(gè)基因或隨機(jī)選擇的基因組合顯示出更好的靈敏度和特異性。在本發(fā)明中，框架的靈敏度反映在一個(gè)基因在疾病狀態(tài)下相對(duì)于其在正常狀態(tài)下所表現(xiàn)出的基因表達(dá)的成倍差異中。特異性可以反映在對(duì)所關(guān)注的基因表達(dá)信號(hào)相關(guān)性的統(tǒng)計(jì)測量中。例如，標(biāo)準(zhǔn)差就是這樣一個(gè)有用的度量。當(dāng)考慮將一組基因摻入到某一框架中時(shí)，表達(dá)測量值的標(biāo)準(zhǔn)差越小其特異性越大。其他檢測變量，如相關(guān)系數(shù)也包括在使用范圍內(nèi)。
最優(yōu)選地，建立基因表達(dá)框架的方法是運(yùn)用優(yōu)化算法，如在確定股票投資中廣泛使用的平均方差算法的。這一方法在同日期的Tim Jatkoe等人標(biāo)題為“籠統(tǒng)投資選擇”的同時(shí)待審的專利申請(qǐng)中有詳細(xì)表述。該方法實(shí)質(zhì)上要求一套輸入值(財(cái)務(wù)應(yīng)用中的庫存，這里指通過強(qiáng)度測定的表達(dá))，其可優(yōu)化收到的返回值(例如形成的信號(hào))以進(jìn)行應(yīng)用，同時(shí)最小化返回值的可變性。許多商業(yè)軟件程序適合進(jìn)行這樣的操作。在整個(gè)說明書中稱為“Wagner軟件”的“Wagner聯(lián)合均值-方差優(yōu)化應(yīng)用程序”是優(yōu)選的。這個(gè)軟件使用“Wagner聯(lián)合均值-方差優(yōu)化庫”中的功能，來確定有效邊界和Markowitz意義上優(yōu)選的優(yōu)化框架。
由于該軟件用于金融分析目的，因此使用這種類型的軟件需要將微陣列數(shù)據(jù)進(jìn)行轉(zhuǎn)換，使數(shù)據(jù)以股票回報(bào)值和風(fēng)險(xiǎn)測量值的方式輸入處理。例如，當(dāng)Wagner軟件與微陣列強(qiáng)度測定協(xié)同應(yīng)用時(shí)，要運(yùn)用以下的數(shù)據(jù)轉(zhuǎn)換方法。
首先通過確定那些其表達(dá)至少顯示出某種微小差異水平的基因，對(duì)基因進(jìn)行預(yù)選擇。優(yōu)選的預(yù)選擇過程如下所述。選擇一個(gè)基線組。典型的基線組包含來自未患有所述疾病的群體的基因。例如，在選擇所用于診斷乳腺癌的框架時(shí)，可以將未患乳腺癌患者的樣本用作制備基線組。一旦選擇了基線組，就可以計(jì)算出基線組樣本中每個(gè)基因的表達(dá)指標(biāo)的算術(shù)平均值和標(biāo)準(zhǔn)差。典型的，這個(gè)指標(biāo)是微陣列記錄的熒光強(qiáng)度。隨后將通過計(jì)算得到的統(tǒng)計(jì)學(xué)數(shù)據(jù)用于計(jì)算每個(gè)基因的基線值(X*標(biāo)準(zhǔn)差+均值)。這就是基因的基線記錄，其它所有樣本都要與其相比較。X是由制定框架的人選擇出來的嚴(yán)格變量。X的數(shù)值越大就越嚴(yán)格。優(yōu)選的X數(shù)值范圍是0.5-3，更優(yōu)選的是2-3，最優(yōu)選的是3。
隨后計(jì)算每個(gè)實(shí)驗(yàn)樣本(那些所關(guān)注的樣本)與基線記錄的比值。再將這些比值轉(zhuǎn)換成易于軟件處理的底數(shù)為10的對(duì)數(shù)值。該方法使下調(diào)基因顯示為使用Wagner軟件并按照Markman均值-方差算法所必需優(yōu)化的負(fù)值。
將包含這些轉(zhuǎn)換比值的預(yù)處理數(shù)據(jù)作為輸入替代用于金融分析目的的Wagner軟件中通常使用的資產(chǎn)回報(bào)值。
一旦制定出有效邊界，對(duì)于一個(gè)給定的輸入水平值(返回值)或?qū)?yīng)于邊界上的一個(gè)點(diǎn)的方差，就可以選擇出一個(gè)優(yōu)化框架。這些輸入值或方差是制定框架的人預(yù)先確定的標(biāo)準(zhǔn)數(shù)集。換句話說，尋求最適框架的人決定一個(gè)可接受的輸入水平(表明靈敏度)或者一個(gè)給定的方差水平(表明特異性)，選擇位于該輸入水平或方差相應(yīng)的有效邊界選擇基因。當(dāng)選定了輸入水平或方差，Wagner軟件就能夠選擇出這樣的基因。Wagner軟件可以像對(duì)股票籠統(tǒng)投資中每支股票所做的那樣，分配所述框架中每個(gè)基因的分量。
將患者樣本框架中的基因中的表達(dá)，與用于構(gòu)建所述框架的差異表達(dá)基因的計(jì)算值相比較，就可以確定該樣本是否患有該框架所診斷的疾病。優(yōu)選的，首先通過將在選擇框架的過程中給每個(gè)基因分配的權(quán)重乘以框架中每個(gè)基因的強(qiáng)度值，加和得到框架值。然后用(Y*標(biāo)準(zhǔn)差+基線組中框架的均值)計(jì)算臨界值，其中Y是一個(gè)嚴(yán)格數(shù)值，具有與上述X相同的含義。一個(gè)樣本具有的框架值大于基線組的框架值，則此樣本就被歸為患病類。需要時(shí)，可以根據(jù)已知的統(tǒng)計(jì)學(xué)方法重復(fù)上述過程提高可信度水平。
任選的，可以重復(fù)進(jìn)行上述過程直到獲得預(yù)期的最高準(zhǔn)確度。
框架選擇的過程和未知量的表征過程概括如下1.選擇基線組2.計(jì)算基線組樣本中每個(gè)基因的均值和標(biāo)準(zhǔn)差3.計(jì)算每個(gè)基因的(X*標(biāo)準(zhǔn)差+均值)。這就是基線記錄，其它所有的樣本都要與這個(gè)基線記錄相比較。X是一嚴(yán)格變量，X數(shù)值越大就越嚴(yán)格。
4.計(jì)算根據(jù)步驟3得到的每個(gè)實(shí)驗(yàn)樣本對(duì)基線記錄的比值。
5.轉(zhuǎn)換數(shù)值，使值小于1的比值成為負(fù)數(shù)(例如使用底數(shù)為10的對(duì)數(shù))。(現(xiàn)在下調(diào)的基因有正確的負(fù)值以符合MV優(yōu)化的需要)。
6.將這些轉(zhuǎn)換的比值作為輸入值，替換軟件應(yīng)用中通常使用的資產(chǎn)回報(bào)值。
7.該軟件將標(biāo)繪出有效的邊界線，并且返回一組沿此有效邊界線上任意一點(diǎn)的優(yōu)化框架。
8.在此有效邊界線上選擇一個(gè)所需的返回值或者方差9.通過將在選擇框架的算法中所給定的權(quán)重乘以每個(gè)基因的強(qiáng)度值，加和計(jì)算每個(gè)樣本的框架值。
10.將基線組的框架值的均值加上Y與基線組的框架值的標(biāo)準(zhǔn)差的乘積，計(jì)算出臨界值。大于該臨界值的數(shù)值應(yīng)被歸為實(shí)驗(yàn)組。
11.任選的，可以重復(fù)進(jìn)行上述過程，直到獲得預(yù)期的最佳準(zhǔn)確度。
可選擇的，首先通過確定那些其表達(dá)至少顯示出某種微小差異水平的基因，對(duì)基因進(jìn)行預(yù)選擇。在這個(gè)可選擇的方法中，預(yù)選擇優(yōu)選的以1≤|(μt-μn)(σt+σn)|]]>給出的閾值為基礎(chǔ)，其中μt是已知患病或有該疾病的樣本子集的均值，μn是正常樣本子集的均值，σt+σn表示聯(lián)合標(biāo)準(zhǔn)差。通過對(duì)根據(jù)0.5≤|(μt-MAXn)(σt+σn)|]]>得到的數(shù)據(jù)的預(yù)選擇，噪音中止點(diǎn)的信號(hào)也能在其中使用。這就確?；诓町愓{(diào)節(jié)進(jìn)行預(yù)選擇的基因具有臨床上的顯著差異。即超過了適于測定各種臨床參數(shù)的儀器產(chǎn)生的噪音水平。對(duì)于根據(jù)這些標(biāo)準(zhǔn)預(yù)選的每個(gè)標(biāo)記物建立一個(gè)矩陣，其中列表示樣本，行表示標(biāo)記物，并且每個(gè)元件都是根據(jù) 對(duì)該標(biāo)記物的表達(dá)進(jìn)行標(biāo)準(zhǔn)化后所得到的強(qiáng)度測量值，其中I代表強(qiáng)度測量值。
也可以通過設(shè)置附加的邊界條件來定義優(yōu)化框架。例如，框架的大小可限定為固定的值域或標(biāo)記物的數(shù)目。這或者可以通過制定更為嚴(yán)格的預(yù)選標(biāo)準(zhǔn)(例如以0.8≤|(μt-MAXn)(σt+σn)|]]>代替0.5≤|(μt-MAXn)(σt+σn)|]]>)或通過使用程序特征如限制組合大小來實(shí)現(xiàn)。例如可以設(shè)置邊界條件使有效邊界線從最優(yōu)化的10個(gè)基因中選出。也可以使用所有的預(yù)選基因確定有效邊界線后再限定選擇基因的數(shù)目(例如不超過10個(gè))。
選擇框架的過程也包括應(yīng)用探索式的規(guī)則。優(yōu)選的，這樣的規(guī)則是基于生物學(xué)和對(duì)用于產(chǎn)生臨床結(jié)果的技術(shù)理解的基礎(chǔ)上制定的。更優(yōu)選的是，它們應(yīng)用到優(yōu)選方法的輸出數(shù)據(jù)中。例如，選擇框架的均方差法可以應(yīng)用于乳腺癌樣本中大量差異表達(dá)基因的微陣列數(shù)據(jù)。這種方法得到的輸出數(shù)據(jù)將會(huì)是一套優(yōu)選的基因，包括既在外周血中表達(dá)也在患病乳腺組織中表達(dá)的一些基因。如果這種檢測方法用到的樣本來自外周血，并且某些基因既在乳腺癌個(gè)例也在外周血中差異表達(dá)，那么就可以應(yīng)用探索式的規(guī)則，從排除了那些在外周血中差異表達(dá)的基因而得到的有效邊界線上選擇框架。當(dāng)然，這個(gè)規(guī)則可以在形成有效邊界線前應(yīng)用，例如，將該規(guī)則應(yīng)用到數(shù)據(jù)的預(yù)先選擇中。
可以應(yīng)用其他與未有定論的生物學(xué)問題無必然聯(lián)系的探索式規(guī)則。例如，可以應(yīng)用只有給定百分比的框架可用特定的基因表示的規(guī)則。商業(yè)上可購買的軟件如Wagner軟件就提供了這類型的探索式規(guī)則。這是非常有用的，例如，除了正確度和精密度(例如，預(yù)支專利轉(zhuǎn)讓費(fèi))等，其他因素也會(huì)影響包括一個(gè)或多個(gè)基因的可用性。
本發(fā)明的一個(gè)方法包括如上所述將基因表達(dá)分布圖與多種基因(或分布圖)比較以進(jìn)行診斷。每個(gè)基因的基因表達(dá)分布圖包括固定于如計(jì)算機(jī)可讀的媒介物上的框架。這可以采取很多形式。例如，可以建立一個(gè)輸入表示疾病的信號(hào)范圍(例如強(qiáng)度測定值)的表格?？梢詫⒉±膶?shí)際數(shù)據(jù)與表格中的值進(jìn)行比較以確定該患者的樣品屬于是正常的或患病的。在一個(gè)改進(jìn)的具體實(shí)例中，表達(dá)信號(hào)的模式(例如熒光強(qiáng)度)以數(shù)字或圖形方式記錄。再將與病例樣本相結(jié)合使用的框架中的基因表達(dá)模式與上述表達(dá)模式比較。然后利用模式比較軟件確定該病例樣本是否具有表示所討論的疾病的模式。當(dāng)然這些比較也可用于確定該病例的結(jié)果是否正常。再將樣本的表達(dá)分布圖與正?；?qū)φ占?xì)胞的框架相比較。如果樣本表達(dá)模式與結(jié)腸直腸癌的表達(dá)模式一致，那么(無抵消性的醫(yī)學(xué)理由)該病例就被診斷為結(jié)腸直腸癌陽性。如果樣本表達(dá)模式與正?；?qū)φ占?xì)胞的表達(dá)模式一致，那么該病例就被診斷為結(jié)腸直腸癌陰性。
許多公知的識(shí)別模式的方法是適用的。下列參考提供了一些例子加權(quán)閾值Weighted VotingGolub，TR.，Sclonim，DK.，Tamaya，Huard，C.，Gaasenbeek，M.，Mesirov，JP，Coller，H.，Loh，L.，Downing，JR.，Caligiuri，MA.，Bloomfield.，CD.，Lander，ES.癌癥的分子分類利用基因表達(dá)檢測的分類發(fā)現(xiàn)和分類預(yù)測?？茖W(xué)Science 286531-537，1999支持矢量機(jī)器Support Vector MachineSu，Al.，Welsh，JB.，Sapinoso，LM.，Kern，SG.，Dimitrov，P.，Lapp，H.，Schultz，G.，Powell，SM.，Moskaluk，CA.，F(xiàn)rierson，HF.Jr，Hampton，GM.利用基因表達(dá)信號(hào)的人類癌癥的分子分類。癌癥研究Cancer Research 617388-93，2001Ramaswamy，S.，Tamayo，P.，Rifkin，R.，Mukherjee，S.，Yeang，GH.，Angelo，M.，Ladd，C.，Reich，M.，Latulippe，E.，Mesirov，JP.，Poggio，T.，Gerald，W，Loda，M.，Lander，ES.，Gould，TR利用腫瘤基因表達(dá)信號(hào)的多種類癌癥診斷。美國國家科學(xué)院院刊Proceedingsof the National Academy of Sciences of the USA 9815149-15154，2001K-最鄰近值K-nearest NeighborsRamaswamy，S.，Tamayo，P.，Rifkin，R.，Mukherjee，S.，Yeang，GH.，Angelo，M.，Ladd，C.，Reich，M.，Latulippe，E.，Mesirov，JP.，Poggio，T.，Gerald，W.，Loda，M.，Lander，ES.，Gould，TR.利用腫瘤基因表達(dá)信號(hào)的多種類癌癥診斷。美國國家科學(xué)院院刊Proceedingsof the National Academy of Sciences of the USA 9815149-15154，2001相關(guān)系數(shù)Correlation Coefficientsvan’t Veer LJ，Dai H，van de Vijver MJ，He YD，Hart AA，Mao M，Peterse HL，vander Kooy K，Marton MJ，Witteveen AT，Schreiber GJ，Kerkhoven RM，Roberts C，LinsleyPS，Bemards R，F(xiàn)riend SH.基因表達(dá)分布圖預(yù)測乳腺癌的臨床結(jié)果。自然Nature.2002 Jan 31415(6871)530-6本發(fā)明的基因表達(dá)分布圖也可以與其他在癌癥診斷、預(yù)測、或治療監(jiān)測中所使用的非遺傳的診斷方法聯(lián)合使用。例如，在一些情況中，將如上所述的基于基因表達(dá)能力的診斷方法與從如血清蛋白標(biāo)記(例如癌胚性抗原)的傳統(tǒng)標(biāo)記物中得到的數(shù)據(jù)結(jié)合使用是很有益的。這樣存在的標(biāo)記物范圍包括如CA19-9，CA125，CK-BB和鳥苷酸環(huán)化酶C的分析物。在這樣的一個(gè)方法中，對(duì)治療患者周期性采血，然后將血樣作上述血清標(biāo)記之一的酶聯(lián)免疫檢測。當(dāng)標(biāo)記的濃度暗示了腫瘤復(fù)發(fā)或治療失敗時(shí)，可以隨后對(duì)樣本源作基因表達(dá)分析。如果存在所懷疑的腫塊，就進(jìn)行更好的針吸采樣并如上所述對(duì)取自腫塊的細(xì)胞進(jìn)行基因表達(dá)分布圖分析?？蛇x擇的，組織樣本可以取自剛摘取腫瘤的組織的鄰近區(qū)域。當(dāng)其他檢測結(jié)果不確定時(shí)，這種方式特別有用。
當(dāng)其它診斷的可靠性值得懷疑時(shí)，聯(lián)合使用遺傳標(biāo)記和其他診斷是最優(yōu)選的。例如，眾所周知CEA血清水平在相當(dāng)程度上受到與患者癌癥狀態(tài)毫無關(guān)系的因子的影響。當(dāng)一個(gè)患者被監(jiān)測到在接受結(jié)腸癌治療后顯示常規(guī)CEA檢測高水平時(shí)，那聯(lián)合進(jìn)行基因表達(dá)/CEA檢測是有益的。
本發(fā)明的制品包括用于治療、診斷、預(yù)測和其它疾病評(píng)估的基因表達(dá)分布圖的表示物。這些分布圖表示物簡化為可被機(jī)器自動(dòng)可讀的媒介物，如計(jì)算機(jī)可讀媒介(磁性的，光學(xué)的及類似的)。制品也包括在這種媒介中評(píng)估基因表達(dá)分布圖的指令說明。例如，制品可能包括具有用于比較上述基因組合的基因表達(dá)分布圖的計(jì)算機(jī)指令的CD ROM。制品可能也含有基因表達(dá)分布圖的數(shù)字記錄以用于與病例樣本的基因表達(dá)數(shù)據(jù)進(jìn)行比較。可選擇的，所述的分布圖可以不同的表現(xiàn)格式記錄。圖形記錄就是這樣的一種格式。上文提到的“GENESPRING”和“DISCOVER”計(jì)算機(jī)程序中整合的聚類算法就可很好的協(xié)助這些數(shù)據(jù)形象化。
根據(jù)本發(fā)明生產(chǎn)制造的不同類型的物品是指用于揭示基因表達(dá)分布圖的媒介物或格式化檢測。這些可以包括，例如微陣列，其中將序列互補(bǔ)物或探針固定于基片上，它與表示所關(guān)注基因的序列聯(lián)合建立一個(gè)易讀行列式?？蛇x擇的，根據(jù)本發(fā)明的制品可以被制作成進(jìn)行雜交、擴(kuò)增，以及形成表示所關(guān)注的基因表達(dá)水平的信號(hào)以用來檢測結(jié)腸直腸癌的試劑盒。
根據(jù)本發(fā)明制備的試劑盒包括確定基因表達(dá)分布圖的格式化檢測。這就包括了進(jìn)行檢測所需的如試劑和說明指令等所有或部分材料。
本發(fā)明進(jìn)一步用下列非限定性的實(shí)施例說明。
實(shí)施例根據(jù)本發(fā)明的基因分析是通過參考GenBank數(shù)據(jù)庫中的基因ID號(hào)鑒定的。它們與編碼產(chǎn)生蛋白質(zhì)或肽的全長核苷酸序列典型相關(guān)。本領(lǐng)域的技術(shù)人員公認(rèn)全長核苷酸序列的鑒定不是一個(gè)分析觀點(diǎn)所必需的。即可以根據(jù)公知的為評(píng)估相關(guān)基因表達(dá)設(shè)計(jì)探針的原理進(jìn)行部分序列或ESTs選擇。
實(shí)施例1-樣品處理和LCM收集接受結(jié)腸直腸腫瘤外科手術(shù)患者的27個(gè)新鮮冷凍組織樣本。19個(gè)樣本是結(jié)腸直腸惡性腫瘤實(shí)例，8個(gè)樣本是正常的結(jié)腸粘膜。組織在獲得后的20-30分鐘內(nèi)快速液氮冷凍，并隨后貯存于-80℃。為了激光捕捉，將樣本切片(6μm)，一切片封固于玻璃載片上，另一切片封固于已被固定于玻璃載片(Micro SlidesColorfrost，VWR Scientific，Media，PA)上的膜(P.A.L.M.)上.封固于玻璃載片上的切片在冷的丙酮中固定，并用Mayer’s蘇木精(Sigma，St.Louis，MO)染色.病理學(xué)家為診斷和定級(jí)分析樣本。由附帶的使用Dukes分級(jí)的外科病理學(xué)和臨床報(bào)告進(jìn)行臨床階段評(píng)估。封固于膜上的切片在100％乙醇中固定5分鐘，在曙紅/100％乙醇(100μg曙紅溶于100ml脫水乙醇)中復(fù)染1分鐘，快速在100％乙醇中浸泡一次以除去未結(jié)合染料，空氣中干燥10分鐘。
結(jié)腸直腸腺癌中的兩個(gè)為等級(jí)1，10個(gè)為等級(jí)2，5個(gè)為等級(jí)3。一個(gè)惡性腫瘤樣本是盲腸的類癌瘤，另一個(gè)是轉(zhuǎn)移的黑色素瘤的損傷。兩個(gè)腺癌樣本表現(xiàn)為粘蛋白狀亞型，一個(gè)為章形細(xì)胞亞型。腺癌樣本按Dukes分級(jí)劃分如下Dukes A2，Dukes B5，Dukes C7，Dukes D3。六個(gè)腺癌樣本在手術(shù)前放療過。
在LCM中使用前，膜(LPC-MEMBERANE PEN FOIL 1.35μmNo.8100，P.A.L.M.GrmbH Mikrolaser Technologie，Bemried，Germany)和載片經(jīng)過預(yù)處理以除去RNA酶，并且增強(qiáng)組織樣品在膜上的吸附。簡要的，載片用DEPH2O洗滌，膜用RNase AWAY(Molecular Bioproducts，Inc.，San Diego，CA)洗滌并用DEP H2O沖洗。膜粘附到玻璃載片上后，載片于120℃烘烤8小時(shí)，用TI-SAD處理(Diagnostic Products Corporation，Los Angeles，CA，以1∶50溶于DEPH2O，用脫脂棉過濾)，+37℃孵育30分鐘。使用前，立即將10μl小份的RNA酶抑制劑溶液(RNA酶蛋白質(zhì)抑制劑2500U＝33U/μl N211A，Promega GmbH，Mannheim，Germany，0.5μl溶于400μl冷的含有0.15mol NaCl，10mmol TrispH8.0，0.25mmol二硫蘇糖醇的溶液)鋪展到膜上封固組織樣本的位置。
封固于膜上的組織切片用于LCM。利用PALM自動(dòng)微光束技術(shù)(P.A.L.M.Microlaser Technologie，Carl Zeiss，Inc.，Thornwood，NY)可捕捉的約2000個(gè)上皮細(xì)胞樣品，并連接到Zeiss Axiovert 135顯微鏡中(Carl Zeiss Jena GmbH，Jena，Germany)。正常粘膜的外周基質(zhì)和癌癥樣本中偶然的干擾基質(zhì)成分都包括在內(nèi)。捕捉到的細(xì)胞置于100％乙醇的小管中于-80℃保存。
實(shí)施例2-RNA提取和擴(kuò)增利用Zymo-Spin柱(Zymo Research，Orange，CA92867)提取通過LCM捕捉的細(xì)胞的總RNA。將約2ng總RNA重懸于10μl水并且利用T7 RNA聚合酶進(jìn)行兩輪擴(kuò)增直至擴(kuò)增的RNA產(chǎn)量約為50μg。
實(shí)施例3-cDNA微陣列雜交和定量利用一套由約20,000個(gè)人cDNA克隆組成的cDNA微陣列檢測樣本。將約30個(gè)植物基因印跡到微陣列上作為非特異雜交對(duì)照。利用LCM捕捉細(xì)胞的5μg aRNA合成Cy3標(biāo)記的cDNA探針。探針用Qiagen’s核苷酸去除柱(Nucleotide Removal Columns)純化然后經(jīng)14-16小時(shí)雜交到微陣列上。載片在掃描前經(jīng)過洗滌并于空中干燥。掃描微陣列的Cy3熒光并用ImaGene軟件(Biodiscovery，Los Angeles，CA)定量。對(duì)于每個(gè)cDNA克隆，進(jìn)行重復(fù)點(diǎn)和重復(fù)陣列的四次測量并將所得強(qiáng)度值平均。
利用第三代微陣列點(diǎn)樣儀(Molecular Dynamics)將cDNAs印跡到氨基硅烷涂層的載片上(Corning)。cDNAs經(jīng)PCR擴(kuò)增，純化(Qiagen PCR純化試劑盒)，并與10M NaSCN印跡緩沖液1∶1混合。雜交前微陣列在異丙醇中室溫孵育10分鐘。探針于95℃孵育2分鐘，室溫孵育5分鐘，然后加到三個(gè)平行測定的載片上。用DPX(Fluka)將蓋片密封到載片上并于42℃孵育過夜。然后將載片在1×SSC/0.2％SDS和0.1×SSC/0.2％SDS中于55℃洗滌5分鐘，0.1×SSC中浸潤并干燥后用第三代微陣列掃描儀(Molecular Dynamics)掃描。用AUTOGENE軟件(Biodiscovery，Los Angeles)分析每個(gè)點(diǎn)的熒光強(qiáng)度。
對(duì)芯片強(qiáng)度進(jìn)行線性標(biāo)準(zhǔn)化使得每個(gè)芯片的強(qiáng)度記錄75th個(gè)百分點(diǎn)相當(dāng)于值100。芯片上的每個(gè)基因通過將該基因的強(qiáng)度記錄除以所有樣本的基因表達(dá)值記錄的中間值標(biāo)準(zhǔn)化。在聚類前，那些至少在一個(gè)樣本中不具有100或更大的強(qiáng)度記錄的基因被濾掉了以限制對(duì)相似性量度的背景影響。選擇一套6255個(gè)基因作聚類分析。利用作為相似性測量的相關(guān)度進(jìn)行分級(jí)聚類，使具有表現(xiàn)正變化同時(shí)不考慮其負(fù)變化(Silicon Genetic，Sunnyville，CA)的基因的樣品聚類到一起。系統(tǒng)圖的每一個(gè)主要節(jié)點(diǎn)可被認(rèn)為是樣本的一個(gè)亞組。通過將每個(gè)腫瘤亞組與正常組進(jìn)行比較鑒定差異表達(dá)的基因。篩選是基于1≤|(μt-μn)(σt+σn)|]]>給出的信噪比測量閾值，其中μt是腫瘤子集的平均數(shù)，μn是正常樣本子集的平均數(shù)，σt+σn表示結(jié)合標(biāo)準(zhǔn)差。組內(nèi)一個(gè)基因的強(qiáng)度記錄的變異系數(shù)必須低于0.33，因?yàn)榛蛱幱诔蓪?duì)方式比較之中。腫瘤組的中間值與正常組的中間值相比大于2或等于2是為上調(diào)，小于或等于0.5倍時(shí)為下調(diào)。如果一個(gè)基因符合所有標(biāo)準(zhǔn)則被選擇出來。在所有的比較中均被篩選出來的基因即被認(rèn)為是在結(jié)腸直腸癌中穩(wěn)定調(diào)節(jié)異常的。利用T檢驗(yàn)假定不等方差計(jì)算統(tǒng)計(jì)學(xué)顯著性p值。用于聚類的一套基因也用軟件包(Partek，St Louis，MO)進(jìn)行主成分分析(PCA)。然后將數(shù)據(jù)投射到簡化的三維空間。正常的和結(jié)腸直腸腫瘤的樣本以映射表達(dá)水平表現(xiàn)出來。
建立有很大上調(diào)差異的基因列表以用來區(qū)別腫瘤和正常樣本。利用0.5≤|(μt-MAXn)(σt+σn)|]]>作為信噪比中止點(diǎn)先挑選出123個(gè)基因。以等于或大于1.5的比率作為上調(diào)的最低標(biāo)準(zhǔn)。也包括0.9≤|(μt-μn)(σt+σn)|]]>的基因。已經(jīng)建立了一個(gè)四基因的框架，每個(gè)基因在腫瘤和正常細(xì)胞間都有至少3倍的差異。
結(jié)腸直腸癌中差異表達(dá)的基因。與正常結(jié)腸粘膜相比，39個(gè)基因在所有的腫瘤樣本中差異表達(dá)。除了一個(gè)異常值，37個(gè)基因在所有的腫瘤中顯著下調(diào)。2個(gè)上調(diào)。基因的同一性通過微陣列上cDNA克隆的測序驗(yàn)證。結(jié)果如表1所示。
表1調(diào)節(jié)基因
實(shí)施例4結(jié)腸直腸腫瘤的優(yōu)化框架利用方差優(yōu)化算法生成多重基因基礎(chǔ)的標(biāo)記，其中包括可結(jié)合使用以區(qū)別正常和腫瘤樣本的基因。利用樣本和如實(shí)施例1-3所描述的微陣列進(jìn)行強(qiáng)度檢測。腫瘤和正常樣本間有5倍差異的數(shù)據(jù)先被預(yù)選出來用作分析。根據(jù)這個(gè)標(biāo)準(zhǔn)預(yù)選出來的基因的表達(dá)數(shù)據(jù)隨后作進(jìn)一步使用。每個(gè)基因的強(qiáng)度測量值的平均數(shù)和標(biāo)準(zhǔn)差是以非轉(zhuǎn)移性樣本作為基線計(jì)算出來的。然后對(duì)于每個(gè)基線基因(X值指定為3)計(jì)算差別值X*(標(biāo)準(zhǔn)差+平均值)。這個(gè)值用來確保結(jié)果框架是有說服力的。再用差別值與基線值的比率計(jì)算每個(gè)轉(zhuǎn)移性樣本。這個(gè)比率隨后被轉(zhuǎn)換成常用對(duì)數(shù)。然后這個(gè)數(shù)據(jù)被輸入Wanger軟件，以產(chǎn)生用來選擇4基因框架的有效邊界。這套基因包括一未知序列，前膠原I型，核糖體蛋白的大亞基L21，和纖連蛋白。這些基因確定為Seq.ID.No.42，Seq.ID.No.43，Seq.ID.No.44和Seq.ID.No.45的序列。換句話說，形成框架的基因組合可以為關(guān)于結(jié)腸直腸癌的臨床判定提供有用的診斷信息。當(dāng)使用選自與附加標(biāo)記(遺傳或非遺傳的)相關(guān)的框架的基因組合特別有益。
優(yōu)化的基因框架＞gi|1264443|gb|N92134.1|N92134 za23f09.r1 Soares fetal liver spleenINFLS Homo sapiens cDNA clone IMAGE293417 5’similar to gb|M87908|HUMALNE32 Human carcinoma cell-derived Alu RNA transcript，(rRNA)；gbX57025_mal INSULIN-LIKE GROWTH FACTOR IAPRECURSOR(HUMAN)＞gi|2221047|gb|AA490172.1|AA490172 ab06b08.s1 Stratagene fetalretina 937202 Homo sapiens cDNA clone IMAGE839991 3’similar to gbJ03464PROCOLLAGEN ALPHA 2(I)CHAINPRECURSOR(HUMAN)＞gi|2188912|gb|AA464034.1|AA464034 zx86b09.r1 Soares ovarytumor NbHOT Homo sapiens cDNA clone IMAGE810617 5’similar toSWRL21_HUMAN P46778 60S RIOSOMAL PROTEINL21＞gi|834491|gb|R62612.1|R62612 yi12d01.s1 Soares placenta Nb2HPHomo sapiens cDNA clone IMAGE139009 3’similar to gbX02761-cds1FIBRONECTIN PRECURSOR(HUMAN)；使用不同的標(biāo)準(zhǔn)但相同的方法，通過軟件選出了又一個(gè)四基因框架。這些是Seq.ID.No.42，Seq.ID.No.43，Seq.ID.No.44和Seq.ID.No.45的序列。兩個(gè)基因與第一個(gè)四基因框架重疊。兩個(gè)優(yōu)化框架也可以結(jié)合形成一個(gè)六基因框架。
優(yōu)化的基因框架＞gi|2114953|gb|AA431245.1|AA431245.1 zw78d06.r1Soares_testi_NHT Homo sapiens cDNA cloneIMAGE782315 5’similar towpF36H1.2 CE05814 ANKYRIN LIKE
＞gi|2156172|gb|AA443497.1|AA443497 zw34d03.r1 Soares ovarytumor NbHOT Homo sapiens cDNA clone IMAGE771173＞gi|2221047|gb|2| AA490172 ab06b08.s1 Stratagen fetal retina 937202Homo sapiens cDNA clone IMAGE839991 3’similar to gbJ03464PROCOLLAGEN ALPHA 2(I)CHAINPRECUSOR(HUMAN)＞gi|1264443|gb|N92134.1|N92134 za23f09.r1 Soares fetal liver spleenINFLS Homo sapiens cDNA clone IMAGE293417 5’similar to gb|M87908|HUMALNE32 Human carcinoma cell-derived Alu RNA transcript，(rRNA)；gbX57025_mal INSULIN-LILE GROWTH FACTOR IA PRECURSOR(HUMAN)；
序列表<110>WANG，YIXIN<120>評(píng)估結(jié)腸直腸癌<130>CDS 267 US NP<140>TBD<141>2003-03-21<150>60/368,798<151>2002-03-29<160>49<170>PatentIn version 3.1<210>1<211>1500<212>DNA<213>人<400>1atggcttcga ccaccacctg caccaggttc acggacgagt atcagctttt cgaggagctt 60ggaaaggggg cattctcagt ggtgagaaga tgtatgaaaa ttcctactgg acaaggatat 120gctgccaaaa ttatcaacac caaaaagctt tctgctaggg atcatcagaa actagaaaga 180gaagctagaa tctgccgtct tttgaagcac cctaatattg tgcgacttca tgatagcata 240tcagaagagg gctttcacta cttggtgttt gatttagtta ctggaggtga actgtttgaa 300gacatagtgg caagagaata ctacagtgaa gctgatgcca gtcattgtat acagcagatt 360ctagaaagtg ttaatcattg tcacctaaat ggcatagttc acagggacct gaagcctgag 420aatttgcttt tagctagcaa atccaaggga gcagctgtga aattggcaga ctttggctta 480gccatagaag ttcaagggga ccagcaggcg tggtttggtt ttgctggcac acctggatat 540ctttctccag aagttttacg taaagatcct tatggaaagc cagtggatat gtgggcatgt 600ggtgtcattc tctatattct acttgtgggg tatccaccct tctgggatga agaccaacac 660agactctatc agcagatcaa ggctggagct tatgattttc catcaccaga atgggacacg 720gtgactcctg aagccaaaga cctcatcaat aaaatgctta ctatcaaccc tgccaaacgc 780atcacagcct cagaggcact gaagcaccca tggatctgtc aacgttctac tgttgcttcc 840atgatgcaca gacaggagac tgtagactgc ttgaagaaat ttaatgctag aagaaaacta 900aagggtgcca tcttgacaac tatgctggct acaaggaatt tctcagcagc caagagtttg 960ttgaagaaac cagatggagt aaaggagtca actgagagtt caaatacaac aattgaggat1020gaagatgtga aagcacgaaa gcaagagatt atcaaagtca ctgaacaact gatcgaagct1080atcaacaatg gggactttga agcctacaca aaaatctgtg acccaggcct tactgctttt1140gaacctgaag ctttgggtaa tttagtggaa gggatggatt ttcaccgatt ctactttgaa1200aatgctttgt ccaaaagcaa taaaccaatc cacactatta ttctaaaccc tcatgtacat1260ctggtagggg atgatgccgc ctgcatagca tatattaggc tcacacagta catggatggc1320agtggaatgc caaagacaat gcagtcagaa gagactcgtg tgtggcaccg ccgggatgga1380
aagtggcaga atgttcattt tcatcgctcg gggtcaccaa cagtacccat caagccaccc1440tgtattccaa atgggaaaga aaacttctca ggaggcacct ctttgtggca aaacatctga1500<210>2<211>5761<212>DNA<213>人<400>2cacaccgcag tatgcggtgc cctttactct gagctgcgca gccggccggc cggcgctggt 60tgaacagact gccgctgtac tggcgtggcc tggagggact cagcaaattc tcctgccttc 120aacttggcaa cagttgcctg gggtagctct acacaactct gtccagccca cagcaatgat 180tccagaggcc atggggagtg gacagcagct agctgactgg aggaatgccc actctcatgg 240caaccagtac agcactatca tgcagcagcc atccttgctg actaaccatg tgacattggc 300cactgctcag cctctgaatg ttggtgttgc ccatgttgtc agacaacaac aatccagttc 360cctcccttcg aagaagaata agcagtcagc tccagtctct tccaagtcct ctctagatgt 420tctgccttcc caagtctatt ctctggttgg gagcagtccc ctccgcacca catcttctta 480taattccttg gtccctgtcc aagatcagca tcagcccatc atcattccag atactcccag 540ccctcctgtg agtgtcatca ctatccgaag tgacactgat gaggaagagg acaacaaata 600caagcccagt agctctggac tgaagccaag gtctaatgtc atcagttatg tcactgtcaa 660tgattctcca gactctgact cttctttgag cagcccttat tccactgata ccctgagtgc 720tctccgaggc aatagtggat ccgttttgga ggggcctggc agagttgtgg cagatggcac 780tggcacccgc actatcattg tgcctccact gaaaactcag cttggtgact gcactgtagc 840aacccaggcc tcaggtctcc tgagcaataa gactaagcca gtcgcttcag tgagtgggca 900gtcatctgga tgctgtatca cccccacagg gtatcgagct caacgcgggg ggaccagtgc 960agcacaacca ctcaatctta gccagaacca gcagtcatcg gcggctccaa cctcacagga1020gagaagcagc aacccagccc cccgcaggca gcaggcgttt gtggcccctc tctcccaagc1080cccctacacc ttccagcatg gcagcccgct acactcgaca gggcacccac accttgcccc1140ggcccctgct cacctgccaa gccaggctca tctgtatacg tatgctgccc cgacttctgc1200tgctgcactg ggctcaacca gctccattgc tcatcttttc tccccacagg gttcctcaag1260gcatgctgca gcctatacca ctcaccctag cactttggtg caccaggtcc ctgtcagtgt1320tgggcccagc ctcctcactt ctgccagcgt ggcccctgct cagtaccaac accagtttgc1380cacccaatcc tacattgggt cttcccgagg ctcaacaatt tacactggat acccgctgag1440tcctaccaag atcagccagt attcctactt atagttggtg agcatgaggg aggaggaatc1500atggctacct tctcctggcc ctgcgttctt aatattgggc tatggagaga tcctccttta1560ccctcttgaa atttcttagc cagcaacttg ttctgcaggg gcccactgaa gcagaaggtt1620tttctctggg ggaacctgtc tcagtgttga ctgcattgtt gtagtcttcc caaagtttgc1680cctattttta aattcattat ttttgtgaca gtaattttgg tacttggaag agttcagatg1740
cccatcttct gcagttacca aggaagagag attgttctga agttaccctc tgaaaaatat1800tttgtctctc tgacttgatt tctataaatg cttttaaaaa caagtgaagc ccctctttat1860ttcattttgt gttattgtga ttgctggtca ggaaaaatgc tgatagaagg agttgaaatc1920tgatgacaaa aaaagaaaaa ttactttttg tttgtttata aactcagact tgcctatttt1980attttaaaag cggcttacac aatctccctt ttgtttattg gacatttaaa cttacagagt2040ttcagttttg ttttaatgtc atattatact taatgggcaa ttgttatttt tgcaaaactg2100gttacgtatt actctgtgtt actattgaga ttctctcaat tgctcctgtg tttgttataa2160agtagtgttt aaaaggcagc tcaccatttg ctggtaactt aatgtgagag aatccatatc2220tgcgtgaaaa caccaagtat tctttttaaa tgaagcacca tgaattcttt tttaaattat2280tttttaaaag tctttctctc tctgattcag cttaaatttt tttatcgaaa aagccattaa2340ggtggttatt attacatggt ggtggtggtt ttattatatg caaaatctct gtctattatg2400agatactggc attgatgagc tttgcctaaa gattagtatg aattttcagt aatacacctc2460tgttttgctc atctctccct tctgttttat gtgatttgtt tggggagaaa gctaaaaaaa2520cctgaaacca gataagaaca tttcttgtgt atagctttta tacttcaaag tagcttcctt2580tgtatgccag cagcaaattg aatgctctct tattaagact tatataataa gtgcatgtag2640gaattgcaaa aaatatttta aaaatttatt actgaattta aaaatatttt agaagttttg2700taatggtggt gttttaatat tttacataat taaatatgta catattgatt agaaaaatat2760aacaagcaat ttttcctgct aacccaaaat gttatttgta atcaaatgtg tagtgattac2820acttgaattg tgtacttagt gtgtatgtga tcctccagtg ttatcccgga gatggattga2880tgtctccatt gtatttaaac caaaatgaac tgatacttgt tggaatgtat gtgaactaat2940tgcaattata ttagagcata ttactgtagt gctgaatgag caggggcatt gcctgcaagg3000agaggagacc cttggaattg ttttgcacag gtgtgtctgg tgaggagttt ttcagtgtgt3060gtctcttcct tccctttctt cctccttccc ttattgtagt gccttatatg ataatgtagt3120ggttaataga gtttacagtg agcttgcctt aggatggacc agcaagcccc cgtggaccct3180aagttgttca ccgggattta tcagaacagg attagtagct gtattgtgta atgcattgtt3240ctcagtttcc ctgccaacat tgaaaaataa aaacagcagc ttttctcctt taccaccacc3300tctacccctt tccattttgg attctcggct gagttctcac agaagcattt tccccatgtg3360gctctctcac tgtgcgttgc taccttgctt ctgtgagaat tcaggaagca ggtgagagga3420gtcaagccaa tattaaatat gcattctttt aaagtatgtg caatcacttt tagaatgaat3480ttttttttcc ttttcccatg tggcagtcct tcctgcacat agttgacatt cctagtaaaa3540tatttgcttg ttgaaaaaaa catgttaaca gatgtgttta taccaaagag cctgttgtat3600tgcttaccat gtccccatac tatgaggaga agttttgtgg tgccgctggt gacaaggaac3660tcacagaaag gtttcttagc tggtgaagaa tatagagaag gaaccaaagc ctgttgagtc3720attgaggctt ttgaggtttc ttttttaaca gcttgtatag tcttggggcc cttcaagctg3780
tgaaattgtc cttgtactct cagctcctgc atggatctgg gtcaagtaga aggtactggg3840gatggggaca ttcctgccca taaaggattt ggggaaagaa gattaatcct aaaatacagg3900tgtgttccat ccgaattgaa aatgatatat ttgagatata attttaggac tggttctgtg3960tagatagaga tggtgtcaag gaggtgcagg atggagatgg gagatttcat ggagcctggt4020cagccagctc tgtaccaggt tgaacaccga ggagctgtca aagtatttgg agtttcttca4080ttgtaaggag taagggcttc caagatgggg caggtagtcc gtacagccta ccaggaacat4140gttgtgtttt ctttattttt taaaatcatt atattgagtt gtgttttcag cactatattg4200gtcaagatag ccaagcagtt tgtataattt ctgtcactag tgtcatacag ttttctggtc4260aacatgtgtg atctttgtgt ctcctttttg ccaagcacat tctgattttc ttgttggaac4320acaggtctag tttctaaagg acaaattttt tgttccttgt cttttttctg taagggacaa4380gatttgttgt ttttgtaaga aatgagatgc aggaaagaaa accaaatccc attcctgcac4440cccagtccaa taagcagata ccacttaaga taggagtcta aactccacag aaaaggataa4500taccaagagc ttgtattgtt accttagtca cttgcctagc agtgtgtggc tttaaaaact4560agagattttt cagtcttagt ctgcaaactg gcatttccga ttttccagca taaaaatcca4620cctgtgtctg ctgaatgtgt atgtatgtgc tcactgtggc tttagattct gtccctgggg4680ttagccctgt tggccctgac aggaagggag gaagcctggt gaatttagtg agcagctggc4740ctgggtcaca gtgacctgac ctcaaaccag cttaaggctt taagtcctct ctcagaactt4800ggcatttcca acttcttcct ttccgggtga gagaagaagc ggagaagggt tcagtgtagc4860cactctgggc tcatagggac acttggtcac tccagagttt ttaatagctc ccaggaggtg4920atattatttt cagtgctcag ctgaaatacc aaccccagga ataagaactc catttcaaac4980agttctggcc attctgagcc tgcttttgtg attgctcatc cattgtcctc cactagaggg5040gctaagcttg actgccctta gccaggcaag cacagtaatg tgtgttttgt tcagcattat5100tatgcaaaaa ttcactagtt gagatggttt gttttaggat aggaaatgaa attgcctctc5160agtgacagga gtggcccgag cctgcttcct attttgattt tttttttttt taactgatag5220atggtgcagc atgtctacat ggttgtttgt tgctaaactt tatataatgt gtggtttcaa5280ttcagcttga aaaataatct cactacatgt agcagtacat tatatgtaca ttatatgtaa5340tgttagtatt tctgctttga atccttgata ttgcaatgga attcctactt tattaaatgt5400atttgatatg ctagttattg tgtgcgattt aaactttttt tgctttctcc ctttttttgg5460ttgtgcgctt tcttttacaa caagcctcta gaaacagata gtttctgaga attactgagc5520tatgtttgta atgcagatgt acttagggag tatgtaaaat aatcatttta acaaaagaaa5580tagatattta aaatttaata ctaactatgg gaaaagggtc cattgtgtaa aacatagttt5640atctttggat tcaatgtttg tctttggttt tacaaagtag cttgtatttt cagtattttc5700tacataatat ggtaaaatgt agagcaattg caatgcatca ataaaatggg taaattttct5760g5761
<210>3<211>2129<212>DNA<213>人<400>3ctgtattgag acaaaggaag ggatctgtca gaaagcaaca cttgttatct tgggcttggc 60agcaaggaag aggacaggta gtggagatcc tgcaatctga aaagcagact gaaaggtgac 120aaagaagctg aagatgggtg gtggagagag gtataacatt ccagcccctc aatctagaaa 180tgttagtaag aaccaacaac agcttaacag acagaagacc aaggaacaga attcccagat 240gaagattgtt cataagaaaa aagaaagagg acatggttat aactcatcag cagctgcctg 300gcaggccatg caaaatgggg ggaagaacaa aaattttcca aataatcaaa gttggaattc 360tagcttatca ggtcccaggt tactttttaa atctcaagct aatcagaact atgctggtgc 420caaatttagt gagccgccat caccaagtgt tcttcccaaa ccaccaagcc actgggtccc 480tgtttccttt aatccttcag ataaggaaat aatgacattt caacttaaaa ccttacttaa 540agtacaggta taaaataaga caaatgttta aatttagtta tgttcacggg tagttgtcaa 600ttggtctgaa acaaatttgc tagggaatct atttgtgtag aactaattaa tgtaaaaaaa 660atagaccatc tcgtgttgtg tgcactgtga tataatggta gtatcagtgc aacttaaact 720aatgattgta cttgatatta agtgttctca actgagtaac ttttaagtgg aaaccaagtt 780tagatttggg gagtggtaaa ggaatcagct ttttctattg ttaggggaag acagtaattt 840atcattcatg gaccagtaga ttgttgaaag ttggtgaatc ggattataag cttctagcta 900acacaaggat tcagaattag gtaaacatct gaaggtttag tatattagaa acacccaaac 960cagtaatatg ctaacctgat gcactgctga aagaaaatgt gaatttttcg taataattgc1020attttagtga attgtacagt gggtggaaag ggcatttgga gctcattaga atgagacata1080gtacacccca atggccctgt ttattaaatg tagtggatta agtgtctgtc aacaaataca1140ccaaaaccat tttttataga aacagtattt aatggtcact caatagcttt caaaatacat1200ttttgtatta cagcactgca caagctattc taatagtgct ctggcctcat cattcctgca1260aagcttgctt tggggagttg gataatgtga aaattttaag tacctagggg agaaagagcc1320atgtaaatat ctgtaataaa cttgtagcat atgtaaagtt ttcttggcct ttatcttaca1380aaaatggagt attttagtat gaatttgctg aatgtaagac cgtggactgt tttttataat1440atggcctaat tttaaaggtc caaaataact tgtttttaaa gtttgccctt gtgctaaagt1500gccagtgtat gtatgttata cttgatttgg ttgtaaacta tatttcaaag taaaccctag1560tgtaataagt tttataacta aaaaggttta agctgctaaa actattttta agagatgtga1620aatgcagtat gggactatct ttttttcctc ctctaagccc aaagattaac tagagtccct1680ccaaccttat agattgttgg ctttcacaat cttataacct aggatacagg tagtttcgag1740tatggtgcca gtgatgtttt gtttttgttt ggtcaagggg taggtgcaac ccaatggacc1800acttatgcaa aagatgtaaa ctcttgcata atacattgat aacatgtttt gccaacttta1860aatgcttaaa cataagcgaa accagtagca agtatgtggg tcagcttaaa aattttgatt1920
gttaatgccc tattttctaa tttggcacct cttgatgcct aagcaggtaa gcagatgcct1980aagctgtatt tctccaaata aatcaagatg aagtactgcc caagttaaat attgatagcc2040taaagacaag tttatgtagt acttaatgta catgatatga agcataaaat taaataaaat2100ttttccccat tgaaaaaaaa aaaaaaaaa 2129<210>4<211>3950<212>DNA<213>人<400>4cgagaactag ttttgttccg tgccctctgg actggaacct tttggagaga acccccggca 60ggaccaaccc cgcacccgcc agcaccgcgg caatgtccag caatagtttt ccttacaatg 120agcagtccgg aggaggggag gcgacggagc tgggtcagga ggcgacctca accatttccc 180cctcgggggc cttcggcctc tttagcagcg atttgaagaa gaatgaagat ctaaagcaaa 240tgttagagag caacaaagat tctgctaaac tggatgctat gaagcggatt gttgggatga 300ttgcaaaagg gaaaaatgca tctgaactgt ttcctgctgt tgtgaagaat gtggccagta 360aaaatattga gatcaagaag ttggtatatg tttacctggt tcgatatgct gaagaacagc 420aggatcttgc actcctgtcc ataagcactt ttcagcgagc tctgaaggac ccaaaccaac 480taattcgtgc aagcgctttg agagttctgt caagtattag agtgccaatt attgtaccta 540tcatgatgct tgctattaag gaagcttctg ctgacttatc accatatgtt aggaagaatg 600cagcccatgc aatacaaaaa ttatacagcc ttgatccaga gcagaaggaa atgttaattg 660aagtaattga aaaacttctg aaagataaaa gcacattggt agctggcagt gttgtgatgg 720cttttgaaga agtatgcccg gacagaatag atctgattca taaaaattac cgcaagctat 780gtaacttact agtggatgtt gaagagtggg ggcaggttgt cataatccac atgctaactc 840gatatgctcg gacacagttt gtcagccctt ggaaagaggg tgatgaatta gaagacaatg 900gaaagaattt ctacgaatct gatgatgatc agaaggaaaa gactgacaaa aagaagaagc 960cgtatactat ggatccagat catagactct taattagaaa tacaaagcct ttgcttcaga1020gcaggaatgc tgcggtggtt atggcagttg ctcagctgta ttggcacata tcaccaaaat1080ctgaagctgg cataatttct aaatcactag tgcgtttact tcgtagcaat agggaggtgc1140agtatattgt cctacaaaat atagcaacta tgtcaattca aagaaagggg atgtttgaac1200cttatctgaa gagtttctat gttaggtcaa ctgatccaac tatgatcaag acactgaagc1260ttgaaatttt gacaaacttg gcaaatgaag ccaacatatc aactcttctt cgagaatttc1320agacctatgt gaaaagccag gataaacaat ttgcagcagc cactattcag actataggca1380gatgtgcaac caacatcttg gaagtcactg acacgtgcct caatggcttg gtctgtctgc1440tgtccaacag ggatgaaata gttgttgctg aaagtgtggt tgttataaag aaattactgc1500aaatgcaacc tgcacaacat ggtgaaatta ttaaacatat ggccaaactc ctggacagta1560tcactgttcc tgttgctaga gcaagtattc tttggctaat tggagaaaac tgtgaacgag1620
ttcctaaaat tgcccctgat gttttgagga agatggctaa aagcttcact agtgaagatg1680atctggtaaa actgcagata ttaaatctgg gagcaaaatt gtatttaacc aactccaaac1740agacaaaatt gcttacccag tacatattaa atctcggcaa gtatgatcaa aactacgaca1800tcagagaccg tacaagattt attaggcagc ttattgttcc gaatgaaaag agtggagctt1860taagtaaata tgccaaaaaa atattcctag cacaaaagcc tgcaccactg cttgagtctc1920cttttaaaga tagagatcat ttccagcttg gcaccttatc tcatactctc aacattaaag1980ctactgggta cctggaatta tctaattggc cagaggtggc gcccgaccca tcagttcgaa2040atgtagaagt aatagagttg gcaaaagaat ggaccccagc aggaaaagca aagcaagaga2100attctgctaa gaagttttat tctgaatctg aggaagagga ggactcttct gatagtagca2160gtgacagtga gagtgaatct ggaagtgaaa gtggagaaca aggcgaaagt ggggaggaag2220gagacagcaa tgaggacagc agtgaggact cctccagtga gcaggacagt gagagtggac2280gggagtcagg cctagaaaac aaaagaacag ccaagaggaa ctcaaaagcc aaaggaaaaa2340gtgattctga agatggggag aaggaaaatg aaaaatctaa aacttcagat tcttcaaatg2400acgaatctag ttcaatagaa gacagttctt ccgattctga atcagagtca gaacctgaaa2460gtgaatctga atccagaaga gtcactaagg agaaagaaaa gaaaacaaag caagatagaa2520ctcctcttac caaagatgtt tcacttctag atctggatga ttttaaccca gtatccactc2580cagttgcact tcccacacca gctctttctc caagtttgat ggctgatctt gaaggtttac2640acttgtcaac ttcctcttca gtcatcagtg tcagtactcc tgcatttgta ccaacgaaaa2700ctcacgtgct gcttcatcga atgagtggaa aaggactagc tgcccattat ttctttccaa2760gacagccttg catttttggt gataagatgg tctctataca aataacactg aataacacta2820ctgatcgaaa gatagaaaat atccacatag gggaaaaaaa acttcctata ggcatgaaaa2880tgcatgtttt taatccaata gactctcttg agcctgaggg atccattaca gtttcaatgg2940gtattgactt ttgtgattct actcagactg ccagtttcca gttgtgtacc aaggatgatt3000gcttcaatgt taatattcag ccacctgttg gagaactgct tttacctgtg gccatgtcag3060agaaagattt taagaaagag caaggagtgc taacaggaat gaatgaaact tctgctgtaa3120tcattgctgc accacagaat ttcactccct ctgtgatctt tcagaaggtt gtaaatgtag3180ccaatgtagg tgcagtccct tctggccagg ataatataca caggtttgca gctaaaactg3240tgcacagtgg gtcattgatg ctagtcacag tggaactgaa ggaaggctct acagcccagc3300ttatcataaa cactgagaaa actgtgattg gctctgttct gctgcgggaa ctgaagcctg3360tcctgtctca ggggtaacct gcttacatct ggactttaga atctggcaca caacaaaagt3420gcctggcatc cactactgct gcctttcatt tataataata gcccttccat ctggcagtgg3480gggtagaata cactcttgac attcttgtct cctgctttag aatgctagtg tgtatctatc3540atgtatgcaa tactttcccc ctttttgctt tgctaaccga agagcatata ttttactgtc3600agttgtctca actcttgaat ccatgtggcg ttttctctgt cctgctgctt cttttggcct3660
cctcgttttc cttctctttt tcgacaatgg tagacatgaa tgagatattt aaagttcatt3720ggaaatcttc ttccctacag cagtaagcaa aaattagcaa agagatagtc taaatggcct3780ctcagcttgg tatgtgaaaa tgagatcaca tactttttaa atccaaatac aaaagcatag3840tctctgcaag attttgttct ttgaatttct tgatattgta attgattatt gataactgtc3900atcatgaaat tatctctcaa taataagata aataaactag catatgaatc 3950<210>5<211>5191<212>DNA<213>人<400>5gagaaagaaa aacagctcga gacctcatgc aaagagaaaa ctgagtatct acagaaaatg 60gttcagagga atgaaagata taaacaagat gtggagaggt tctatgaacg gaagcgacat 120ttagatttaa ttgagatgct tgaagcaaaa aggccatggg tggaatatga aaatgttcgt 180caggaatatg aagaagtaaa actagttcgt gaccgagtga aggaagaggt cagaaaactt 240aaagaagggc agattcctat aacatgtcga attgaagaaa tggaaaacga gcgtcacaat 300ttggaggctc gaatcaaaga aaaggcaaca gatattaagg aggcatctca aaaatgcaaa 360cagaagcaag atgttataga aaggaaagat aaacatattg aggaacttca gcaggcttta 420atagtaaagc aaaatgaaga gcttgaccga cagaggagaa taggtaatac ccgcaaaatg 480atagaggatt tgcaaaatga actaaagacc acggaaaact gcgagaatct tcagccccag 540attgatgcca ttacaaatga tctgagacgg attcaggatg aaaaggcatt atgtgaaggc 600gaaataattg ataagcgaag agagagggaa actctagaga aggagaaaaa gagtgtggac 660gatcatattg tacgttttga caatcttatg aatcagaagg aagataagct aagacagaga 720ttccgtgaca cgtatgatgc tgttttatgg ctaagaaata acagagacaa atttaaacaa 780agagtctgtg agcccataat gctcacgatc aatatgaaag ataataaaaa tgccaaatat 840attgaaaatc atattccatc aaatgactta agagcctttg tatttgaaag tcaagaagat 900atggaggttt tcctcaaaga ggttcgtgac aataaaaaat taagagtaaa tgctgttatt 960gctcccaaga gttcatatgc agacaaagca ccttcaagat ctttgaatga acttaaacaa1020tacggatttt tctcttattt gagagaatta tttgatgcac ctgatcctgt aatgagttac1080ctttgctgtc agtatcatat tcatgaagtt cctgtaggaa ctgaaaagac cagagaaaga1140attgaacggg taatacaaga aacccgatta aaacagattt atacagcaga agaaaagtat1200gtggtgaaaa cttcttttta ttcaaacaaa gttatttcta gtaacacatc tctaaaagta1260gcgcagtttc tcactgtcac tgtggaccta gagcagagaa gacacttaga agaacagcta1320aaggaaattc atagaaaatt gcaagcagtg gattcagggt tgattgcctt acgtgaaaca1380agcaaacatc tggagcacaa agacaatgaa cttagacaaa agaagaagga gcttcttgag1440agaaaaacca agaaaagaca actggaacaa aaaatcagtt ccaaactagg aagtttaaag1500ctgatggaac aggatacttg caatcttgaa gaggaagagc gaaaagcaag taccaaaatc1560
aaagaaataa atgttcaaaa agcgaaactt gttaccgaat taacaaacct aataaagatt1620tgtacttctt tgcatataca aaaagtagat ttaattctcc aaaatactac agtgatctct1680gagaagaaca aattagaatc agattatatg gccgcatctt cacaactccg tcttacagag1740caacatttca ttgaattgga tgaaaataga cagagattat tgcagaaatg caaggaactt1800atgaaaagag ctaggcaagt atgtaacctg ggtgcagagc agactcttcc tcaagaatac1860cagacacaag tacccaccat tccaaatgga cacaactcct cactccccat ggttttccaa1920gaccttccaa acacattgga tgaaattgat gctttattaa ctgaagaaag atcaagagct1980tcctgcttca cgggactgaa tcctacaatt gttcaggaat atacaaaaag agaagaagaa2040atagaacagt taactgagga actaaaggga aagaaagttg aactagatca atacagggaa2100aacatttcac aggtaaaaga aaggtggctt aatcctttaa aagagctggt agaaaaaatt2160aatgaaaaat tcagcaattt ttttagttcc atgcagtgtg ctggtgaagt tgatctccat2220acagaaaatg aggaagatta tgataaatat ggaattcgaa ttagagtcaa atttcgaagt2280agtactcaac tgcatgaatt aactcctcat catcaaagtg gaggtgaaag aagtgtttct2340accatgttat acttgatggc acttcaggag ctaaatagat gtccattcag agtagttgat2400gaaatcaatc agggaatgga cccaatcaat gaacggagag tgtttgaaat ggttgtaaat2460actgcctgta aagaaaatac atctcaatac tttttcataa caccaaagct cctgcaaaat2520cttccttatt ctgaaaagat gacagttttg tttgtctaca atggccctca tatgctggaa2580ccaaacacat ggaatttaaa ggctttccaa aggcggcggc gccgtattac attcactcaa2640ccttcttaat aaaagtaaag agagggaact tgggaatttt ttttgttaaa ttctgtttat2700aagtatggct caactgaata aaaggagatt cactaaaacg aaaagcagtt atttttggaa2760acctgctttt aaatacaaat aggttgataa tggaaactat aatgaccttt ccaaaatagc2820agctggtagt aaaagttaag tcttcttcag tcttggttga acttgagttc ttggcactct2880gaccatgagt cattcagttc tcatgttaaa atgtacttaa tattacaaat caaaggtaca2940gtggaagaag ggttaatcac aagaagttac ttatatggta gccctgagct ttaattgcag3000agtaacttta attactttta gagcctaaag atgactctag agcctaagtc ctagtttctc3060ccaatgttat atttaatttt aaaaaattga tatgaaaatg tctaatgtat agtaataatt3120tatgacagat ctagtcattt cttcctatta aaaaagatta ccttatctcc agtaggaaat3180ggaattttat gggcctttaa aagaaagttt tatgaaactt gatgctataa ttttattggt3240atttcaaggg gaaaaaagca ctggggttca aaaatggtag cagaactgct ttgaaatgct3300gcaaggtggc cactagatga tgcaaaatac aaccaaaaga ttgactgaga ataaaattag3360gtgacaaggg tttttaaaga ataacctttt aaagtgtggg ggcaggggtt gctttttttt3420attttattta aagtcaatta tattttacat cttacatttc taaaagcatt ttataattat3480ttttagtaag atttttctta aaatttcata tactggtttc tacaatttat atttgaaatt3540tctcagtgtt atgtaaagag tgatggaaaa gcattgattt ctttaaaacc gtaatgtttt3600tagaacttaa gcctataggg cctttcttac aatgttgatg tacccattat cttagaaaat3660
ctagtttaaa ctgttttctt tcaccgcaaa agaattaaat gggaaaatca tttgtttatc3720tctaagttat actaattagt agaaccaaac aaattatctt cttttaaaaa ataaatctta3780taggaaaata gacagtccaa agtcatgtct ttgaacagtg gattggatct gtgccagtaa3840tgacaaaatt atttttttga cttgcttgcc tgaataaatt gaagaattgc tttcagtttg3900ggttttgtat attcttaagt agccattgaa atttatattc ttaactaggt caaaaaataa3960tgagccataa gtttatgtcc tctcacttag acattttctc tttaaaaagg tattttcttc4020tttataaaca ttttaaaaga gccttccctt cttaaactaa ctccagtgca tgaagtgtga4080aaatatttta aaatgacatt tttactaata tgagcaagtc atgtaaacat tgaagaactt4140ggtaacatat tagtaaatgg atattaccaa atgttttcat cgttaattac tttgcgttcc4200accaaaatat ctttactaaa atgtgcttgg tgtagtttgt ttattgtcta aattagtacc4260agtcatctta tttctgcaaa atgagtatca atgtgaaaaa gacacgtgaa gattaagcat4320gtttgaaaat aaaatggtca attacatttc aatttacata ggccaacaac tgttccatac4380tttgtttgta aacatttaat ttctctactg gacaaaatta atatttggct ttacattgaa4440ttttgagctg tgaagaataa attatgtatc attttagcat attaaacagt agtaagtcta4500gcacatagtc tcagccactt aaaacaaaag tttttttgtt tgtttgtttg tttgtttttt4560tgagatggag tctcactctg ttgcccaggc tggagtgcag tggcgtgatc tcggcttact4620gcaacctccg cctcccgggt tcaagcgatt ctcctgcctc agcctcccaa gtaactggga4680caacaggcgc gtcccaccac acccagctaa ttttttatac ttttagtaga gatggggttt4740cagcatattg gccaggctgg tctcgaactc ctgaccttgt gatccacccg cctcggcctc4800ccaaagtgct gggattatag gcgtgagccc ctgcacccgg ccaaaagttg atttttaatt4860acataaaaat cgtaaaaact tctagtaaaa acttgatttg gtgaatacag ttatatttta4920aaaccttaag gtgacaagca ttttctatgc ctaaatcttc attggtttgc ctggaaagag4980tctctgttaa aagattttcc atattcaaag taaaaggaaa gatttcttgc ttcctaattg5040tcttttggac acatgcctat tttctttgag gtataaacct ttagatgtga aaaatgtaat5100ttcattctgc tattgtgtgt gcttgtgtgt gtgtaattga aaaaactggg aaatcctgct5160ttgttggtaa taaatcaata tttttatatt c 5191<210>6<211>4755<212>DNA<213>人<400>6aagagatctt ccaggctctc agagccctgg gagggcgatt tccaggaaga ccacaatgcc 60aacctctgga ggaggctgga gagagaaggc ctaggccaga gcctgtcagg caactttggc 120aagaccaagt cagccttctc atctctccag aacattcctg agagtctgag aagacacagc 180agcctggagc taggccgggg aacccaggag ggttaccccg ggggcaggcc cacctgtgca 240gtcaacacca aggcagaaga ccctgggagg aaagccgctc ctgacctcgg gagccatctg 300
gaccggcagg tttcctaccc gcggcccgag gggaggaccg gtgcctcggc ttctttcaac 360agcacagacc caagtcccga agagccgcct gccccctcgc acccgcacac atccagtctg 420ggccggaggg ggcccggccc aggcagcgcc tcggctcttc agggctttca gtacgggaag 480ccccactgct cggtgctgga gaaggtctcc aaattcgagc agcgagagca agggagccag 540agaccgagtg tgggcggctc tggttttggc cataactata ggccccacag gaccgtctca 600acttccagta cttctgggaa tgacttcgag gagacaaaag cacacattcg tttctctgag 660tcagctgaac ccctaggcaa cggggagcag cacttcaaaa acggggagct gaagttggaa 720gaggcttccc ggcagccctg cggtcagcag ctgagcggag gagcgtcgga cagcggccgt 780ggcccccaga ggccggacgc tcggctcctc cgtagccaga gcaccttcca gctctccagc 840gagccagaga gggagcccga gtggcgggac aggcccggct cgcccgaatc gcccctgctg 900gatgccccct tcagccgcgc ctaccggaac agcatcaagg acgcacagtc ccgtgtcttg 960ggggccacct cctttcgacg tcgagacctg gagctggggg cgcccgtggc gtcgaggtcc1020tggcggccac ggccttcctc ggcccacgtg gggctgcgga gccccgaggc gtcggcctcc1080gcctccccgc acacgccccg ggagcggcac agcgtgaccc ctgctgaggg cgacctggcc1140aggcccgtgc cccctgccgc ccggagaggt gctcgccggc gcctgactcc cgagcagaag1200aagcgctcct actcggagcc cgagaagatg aacgaggtgg ggatcgtgga ggaggccgaa1260ccggcacccc tgggcccgca gagaaatggg atgcgtttcc cggagagcag cgtggccgac1320cggcgccgtc tcttcgagcg cgatggcaag gcctgctcca cgctcagcct gtcggggccc1380gagctgaagc agttccagca gagcgccctg gcggactaca tccagcgcaa gaccggcaag1440cggcctacct ccgccgccgg ctgcagcctc caggagcccg ggccactgcg tgagcgcgcc1500cagagtgcct acctccagcc cggccccgcg gcgctcgaag gctccggcct cgcctcggcc1560tccagcttga gctcactgcg ggagcccagc ctgcagcccc gcagggaggc cacgctcctg1620ccggccacag ttgcagaaac ccagcaggct ccccgagatc gcagcagctc cttcgccggt1680ggccgccgcc tcggggaacg gcgacgcggg gacctgctta gcggagcaaa cggtggaaca1740aggggcaccc agagagggga tgagaccccc agggagccat cctcctgggg ggccagggcc1800gggaagtcca tgtcggccga ggacctgctg gaacgctcgg acgtccttgc gggccctgtc1860catgtgaggt ccaggtcatc tcccgccacc gcagacaagc gccaggatgt gcttttgggg1920caagacagtg gctttggtct tgtgaaggat ccatgttatt tggctggtcc tggatctagg1980tcactcagtt gttcagaaag aggccaagaa gagatgctgc tgctcttcca ccatctcacc2040cctcgttggg gtggttcagg ctgcaaagcc attggtgatt cctccgttcc tagtgaatgt2100cctggaaccc tggaccatca gaggcaagcc agtaggacac cctgccccag gccaccactg2160gcaggaacgc aagggctggt cacagacacc agggctgcac ccctgacccc aattggcacc2220cctctgcctt cagccattcc ctctggctac tgctcacagg acggtcagac agggcgacag2280cctctcccgc cctacacccc tgccatgatg cacagaagca atggtcacac cctgacccag2340
cctcccggtc caagaggctg tgagggcgat ggcccagagc atggggtaga agagggaacg2400aggaagaggg tctcgctgcc tcagtggcca cctccttctc gagcaaagtg ggcccacgca2460gccagagagg acagccttcc tgaggaatcc tcagcccctg attttgcaaa cctgaagcac2520tatcaaaaac agcagagtct tccaagttta tgcagcactt ctgacccaga cacacctctt2580ggggccccga gcactccagg gaggatctcc ctccgaatat ctgagtctgt cctgcgggac2640tccccgccac ctcatgagga ttatgaagac gaagtgtttg tgagggatcc gcaccccaag2700gccacgtcca gccccacatt tgaacctctt cccccacccc cacctcctcc accgagtcag2760gaaaccccgg tgtatagcat ggatgacttc cctccacctc ctccccacac tgtatgtgag2820gcgcagctgg acagtgagga tcccgagggg ccacgcccca gcttcaacaa actttctaaa2880gtgacaattg caagggaaag gcacatgcct ggtgcagccc atgtggtagg tagtcagaca2940ctggcttcca gactccaaac ttctatcaag ggttcagagg ctgagtccac accaccctcc3000ttcatgagcg ttcacgccca acttgctggg tctcttggtg ggcagccagc acccatccag3060actcaaagcc tcagccatga tccagtcagt ggaactcagg gtttagaaaa gaaagtcagt3120cctgatcctc agaagagttc agaagacatc agaacagagg ctttggccaa ggaaattgtc3180caccaagaca aatctctagc agacattttg gatccagact ccaggctgaa gacaacaatg3240gacctgatgg aaggtttgtt tccccgagat gtgaacttgc tgaaggaaaa cagtgtaaag3300aggaaggcca tacagagaac tgtcagctct tcaggatgtg aaggcaagag gaatgaagac3360aaggaagcag tgagcatgtt ggttaactgc cctgcctact acagtgtgtc tgctcccaag3420gctgagctac tgaacaaaat caaagagatg ccagcagaag tgaatgagga agaggaacag3480gcagatgtca atgaaaagaa ggctgagctc attggaagtc tcacccacaa gctggagacc3540ctccaggagg cgaaggggag cctgctcacg gacatcaagc tcaacaacgc cctgggagaa3600gaggtggagg ctctgatcag cgagctctgc aagcccaatg agtttgacaa gtataggatg3660ttcatagggg atttggacaa ggtggtcaac ctgctgctct ccctctcggg gcgtctagcc3720cgtgttgaga atgtccttag cggccttggt gaagatgcca gtaatgaaga aaggagctct3780ctttacgaga aaaggaagat cctggctggt cagcatgagg atgcccggga gctgaaggag3840aacctggatc gcagggagcg agtagtgctg ggcatcttgg ccaattacct ttcagaggag3900cagctccagg actaccagca cttcgtgaaa atgaagtcca cgctcctcat tgagcaacgg3960aagctggatg acaagatcaa gctgggccag gagcaggtca agtgtctgct ggagagcctg4020ccctcagatt tcattcccaa ggctggggcc ctggctctgc ccccaaacct cacgagtgag4080cccattcctg ctgggggctg tactttcagt ggtattttcc caacattaac ctctccactt4140taacctcttc taaaataccc aaccaaaaga tcactgtttc tctcaacact atttaatctg4200aaaaatgttt cagtacaaac cactgtttga actatctggg ttattggtgt ttgttcctga4260tgaaaggaaa aaaattctct ccaggaggaa gcctttttcc ttcttgccct tcctgattga4320tcttctgaga gctcgaatgc tgctggacac gtaccccttt ctattattac tttgtagtag4380aaagaaagtt aatgaaactg agaactgatt ggagggtgtt tgatcattta gtttttaaca4440
ggctgaggca acatggatca gtgtgtgtcc ccctcaggaa tgtatccaca gtggccttcc4500ttgctggtgg gcagtgtatc ctgatggcag ggtacaagta ccattaatga agggtctgca4560acataaagcc ttaaaaagac acacactaag aaaactgtaa aaccttgaac attgttattt4620atatttttta aaatggaaaa gatcactatg tttgttgtgc taaccactta tttgattctg4680ttttgtggtg gacatagatg attacgtttg agctttgtat tttgtgaaaa ccttaatgaa4740atgaattcca aagat 4755<210>7<211>2045<212>DNA<213>人<400>7gaaacttgac cccggctcat cctgtctctg gctgtggccc ggcaaagcac tgaaaacccc 60tctggtctca gagacagtag gggcagtgcc actttctaca acctgccaac ccacacactg 120gagtaattct gaaaaaaatt attcctaaac tctctaagtg tggacggaga atgagcaagc 180cccagaagta ttttacaacc agagtgggta atgaggaggg ggcttactgg aatcgtcata 240tctctgaata ttgaaaacaa caactaaaaa agtggacctt ctcagaaaaa aagggcagca 300aatgaccaag ggcgcccctt ctggccgtgc ttggcttgag taactgtctc tctttcccca 360cccccatcac agggctttca gtttggcaaa ggaaaagcag ataaaaacag aacattccat 420atgtttcttt ctccatcggc caaaaacatt ttgacacaat gtttgtgaaa cacctttgga 480gaggtgcact tctgaatgct gcctctgccg taaatcctgg ggcaagggat cagcctcttc 540ccaggaacca tcgccttcta taaaccgtga actcaagcag gcattttttt tttcttaccg 600aaaggctgct attgtgcaag ggcacataat gggtctgttg ctcttattgg cttccaaatg 660tgcatggcaa agagagagat gtgggcctag agcagatata ttcagcaagg tgacagcttc 720ccataacaat tctaacactt cttatcttat gtgagaataa aatatttaag ggttgaacct 780tattttgcca aatgtatctt ttctgctttt gaattgggca gaagatttta gcaactatat 840tctacaaatg ttacttataa cacacacaca cacatctgaa atatatgccg aaaattgacg 900tctttgacct cagggagagc acctgtccag gtctgcctaa aggaaatggc tccagtgggt 960ctaaacaacc acatcctatc catggatagg tctagtcata acactttaga gagaatgtca1020gagcaggagg gaggcaagcc gcctcttctc ggccatcaac tgcagatgat gaaagagcgg1080gattcaactt tgttttcttt tcctgtggcc ccagtgaaac ctcctgccct ccctgcacgt1140ctgtgtcttc atttctaaaa tgggggtgat gctttcatat tgacctcacc ccatactacc1200tcacagatgt gttgtgagga ttaataaaat tatgtctatg gtattttcag tttctggaga1260aaaatactta tagacagttt aactattaca tagatatata agtgatctca gtttcttgtt1320tgctgtgata ctaatgtgtt gttttaactt attccataaa atgacagttg tgtcctagcc1380acatcagaca gctatctaag ctctggacta cccctttgtg cagctgaatc actgcagggt1440cgaccatgcc tggtgccaca gccatggttt ccatttctag atgaaaggat ggcctaggac1500
ataggtctca aagactcttg gatcagaatc aggagattag ggaaaacagg atggatacct1560gagcactaac agcagtagac gtagacctct gtcctttacc atctgaggtc ttctggattc1620tttgtggggt taattttgat ttgatgtcat ctgtttgccc ttcatcttgc ttgcaagtgt1680gcatggttca atccctcaca tccaggaaat gaattttgca attgggccag atgctaattt1740gcacgttgat tcaccttctt tgcctttaag cctttttttt cttttttttt ttttttggca1800aatgaatgta ccatttcaac tttgatttta atagtgctag ttgatattgg taataatgct1860aaccaagaga tcaatgccag atttttctct tggggtaagt tagctgaagt catttaaaga1920tggaaaggtg ggaaaattct ttgatatttg atgtcattgt atccacattt gttgtaagac1980atattgcata ccaattataa ttatatcaat taaagttgat aaaagcttca aaaaaaaaaa2040aaaaa2045<210>8<211>2096<212>DNA<213>人<400>8atggagaacg agcctgtagc ccttgaggaa actcagaaga cagatcctgc tatggaacca 60cggttcaaag tggtggattg ggacaaggac ctggtggact ggcgaaagcc tctcctgtgg 120caggtgggcc acttgggaga gaagtacgat gagtgggttc accagccggt gaccaggccc 180atccgcctct tccactcaga cctcattgag ggcctctcta agactgtctg gtacagtgtc 240cccatcatct gggtgcccct ggtgctgtat ctcagctggt cctactaccg aacctttgcc 300cagggcaacg tccgactctt cacgtcattt acaacagagt acacggtggc agtgcccaag 360tccatgttcc ccgggctctt catgctgggg acattcctct ggagcctcat cgagtacctc 420atccaccgct tcctgttcca catgaagccc cccagcgaca gctattacct catcatgctg 480cacttcgtca tgcacggcca gcaccacaag gcacccttcg acggctcccg cctggtcttc 540ccccctgtgc cagcctccct ggtgatcggc gtcttctact tgtgcatgca gctcatcctg 600cccgaggcag tagggggcac tgtgtttgcg gggggcctcc tgggctacgt cctctatgac 660atgacccatt actacctgca ctttggctcg ccgcacaagg gctcctacct gtacagcctg 720aaggcccacc acgtcaagca ccactttgca catcagaagt caggatttgg tatcagcact 780aaattgtggg attactgttt ccacaccctc actccagaga aaccccacct gaagacgcag 840tgacaactcc caccccctcc gtcctgccct cagcccggcc ctggcccctt cccgaccccc 900acccgccatt cagaccccat taagaaggtt ggcttggcca ggcaggatgg gctgtgtccg 960gccctgcagc ctagtggaag gtgctgaggg ggccctgagg caggaccgcc ctcctgaccc1020ctggtaggag ggtcacatcc acttggtgca ggtggccctt ggtgacccac ttcttcctgg1080agcgtccctg cctagagctc agcccacagg actgcttcag gccgtggcca caggtagcag1140ccgcaagggg aaatgaagaa aactgagccc tcgtggccac ctgtgtcacc cttgtgcctt1200agcctcatgg gctgcctagg agctgcctgc acggcacagc tcgctttcac agtcagaagt1260
gggtctgtgg gatctgtggt ccctgtcctc cctgctgtcc cttctgggga ggctttggtg1320gctctgaggt ggacaaagag ctctcgcaag aagagacagc gtgatgcctc ccacagtcca1380ccccagaccc tggggcagcc cctctggccc tgccagctgc ctgcgtcgtt gggcccaggg1440tggctggcag gagtcccagc tgcttgcttt aggacctggc agcttttctt gccgtccctc1500ccctgcctcc agaatcacag cccttctccc caagggaggc tgaggaggct tctccaccag1560tggcagcccc accccgtccc tggccattct tggcctccac cccgctcagg cccctactcg1620ggcgctccca gaaggagcca cctctcagtg cctcacctcc ccctgcctcc cagcctccgc1680agatgaggtt cctgcccctt cctcctcgta accaaaaccc tcactgctcc caggacggtc1740ttatttataa accagataca tgttcttagt ctggtcccag accaaggagc tggtcagacg1800gccctttcta atcctacatg ttgagcttat gtaaaaaatg ttgtttcctc ctgtttttgg1860ttcctttctt acccacaaac cattactact tgaaacttaa aaaactcgcc aagtgtaaag1920gctaaagaga agcagtttga cggaccttgt gatttgtact gtttgctgcg gagctattta1980aagattttgg aataaatata caaaactacg gttgtgaaat aaaaacttaa attgtatatt2040ttgaaaaata aaacactgaa aagaaaccaa caaaaaaaaa aaaaaaaaaa aaaaaa2096<210>9<211>5640<212>DNA<213>人<400>9ggaaacgcag aaaacagaga gaggcattct gagtcatctg actggatgaa gactgttcca 60agttacaacc aaacaaatag ctccatggac tttagaaatt atatgatgag agatgagact 120ctggaaccac tgcccaaaaa ctgggaaatg gcctacactg acacagggat gatctacttc 180attgaccaca ataccaagac aaccacctgg ttggatcctc gtctttgtaa gaaagccaaa 240gcccctgaag actgtgaaga tggagagctt ccttatggct gggagaaaat agaggaccct 300cagtatggga catactatgt tgatcacctt aaccagaaaa cccagtttga aaatccagtg 360gaggaagcca aaaggaaaaa gcagttagga caggttgaaa ttgggtcttc aaaaccagat 420atggaaaaat cacacttcac aagagatcca tcccagctta aaggtgtcct tgttcgagca 480tcactgaaaa aaagcacaat gggatttggt tttactatta ttggtggaga tagacctgat 540gagttcctac aagtgaaaaa tgtgctgaaa gatggtcccg cagctcagga tgggaaaatt 600gcaccaggcg atgttattgt agacatcaat ggcaactgtg tcctcggtca cactcatgca 660gatgttgtcc agatgtttca attggtacct gtcaatcagt atgtaaacct cactttatgt 720cgtggttatc cacttcctga tgacagtgaa gatcctgttg tggacattgt tgctgctacc 780cctgtcatca atggacagtc attaaccaag ggagagactt gcatgaatcc tcaggatttt 840aagccaggag caatggttct ggagcagaat ggaaaatcgg gacacacttt gactggtgat 900ggtctcaatg gaccatcaga tgcaagtgag cagagagtat ccatggcatc gtcaggcagc 960tcccagcctg aactagtgac tatccctttg attaagggcc ctaaagggtt tgggtttgca1020
attgctgaca gccctactgg acagaaggtg aaaatgatac tggatagtca gtggtgtcaa1080ggccttcaga aaggagatat aattaaggaa atataccatc aaaatgtgca gaatttaaca1140catctccaag tggtagaggt gctaaagcag tttccagtag gtgctgatgt accattgctt1200atcttaagag gaggtcctcc ttcaccaacc aaaactgcca aaatgaaaac agataaaaag1260gaaaatgcag gaagtttgga ggccataaat gagcctattc ctcagcctat gccttttcca1320ccgagcatta tcaggtcagg atccccaaaa ttggatcctt ctgaggtcta cctgaaatct1380aagactttat atgaagataa accaccaaac accaaagatt tggatgtttt tcttcgaaaa1440caagagtcag ggtttggctt cagggtgcta ggaggagatg gacctgacca gtctatatat1500attggggcta ttattcccct gggagcagct gagaaagatg gtcggctccg cgcagctgat1560gaactaatgt gcattgatgg aattcctgtt aaagggaaat cacacaaaca agtcttggac1620ctcatgacaa ctgctgctcg aaatggccat gtgttactaa ctgtcagacg gaagatcttc1680tatggagaaa aacaacccga ggacgacagc tctcaggcct tcatttcaac acagaatgga1740tctccccgcc tgaaccgggc agaggtccca gccaggcctg caccccagga gccctatgat1800gttgtcttgc aacgaaaaga aaatgaagga tttggctttg tcatcctcac ctccaaaaac1860aaaccacctc caggagttat tcctcataaa attggccgag tcatagaagg aagtccggct1920gaccgctgtg gaaaactgaa agttggagat catatctctg cagtgaatgg gcagtccatt1980gttgaactgt ctcatgataa cattgttcag ctgatcaaag atgctggtgt caccgtcaca2040ctaacggtca ttgctgaaga agagcatcat ggtccaccat caggaacaaa ctcagccagg2100caaagcccag ccctgcagca caggcccatg ggacagtcac aggccaacca catacctggg2160gacagaagtg ccctagaagg tgaaattgga aaagatgtct ccacttctta cagacattct2220tggtcagacc acaagcacct tgcacagcct gacaccgcag taatttcagt tgtaggcagt2280cggcacaatc agaaccttgg ttgttatcca gtagagctgg agagaggccc ccggggcttt2340ggattcagcc tccgaggggg gaaggagtac aacatggggc tgttcatcct tcgtcttgct2400gaagatggtc ctgccatcaa agatggcaga attcatgttg gtgaccagat tgttgaaatc2460aatggggaac ctacacaagg aatcacacat actcgagcaa ttgagctcat tcaggctggt2520ggaaataaag ttcttcttct tttgaggcca ggaactggct tgatacctga ccatggtttg2580gctccttccg gtctgtgctc ctacgtgaaa cccgagcaac attaaggctt tcagggcttt2640tcttggtctt tccttaaaaa gacttggtga ttgggatatt aataatcctt cgtcttcaaa2700tgtgatttat gatgaacagt caccattacc cccatcttca cattttgctt ccatatttga2760agagtctcac gtgccagtaa ttgaagaatc tttgagagtt cagatatgtg aaaaggcaga2820agaattaaag gacattgtgc ctgaaaagaa aagcacttta aatgaaaatc agcctgagat2880aaagcatcag tctcttctcc agaaaaatgt gagtaagagg gatccaccca gcagtcatgg2940gcacagtaac aagaaaaatc tattaaaagt agaaaatggt gttacacgaa gaggtagatc3000ggttagtccc aaaaagccag ccagtcaaca ttcagaggaa catttggata agattcctag3060
tcctctaaaa aataacccca aaagaagacc cagagatcaa tccctcagcc ccagcaaagg3120ggaaaataaa agttgtcagg tcagcaccag ggcaggctct ggacaagatc agtgcagaaa3180aagcagaggt cggtcggcca gcccaaaaaa gcagcaaaaa attgaaggaa gcaaagctcc3240atcaaatgct gaggccaaat tattagaggg taagagtcga agaatagcag gctatacggg3300cagtaatgct gagcagatcc cagatgggaa ggaaaaatca gacgtcatca ggaaagatgc3360aaagcagaat cagttggaaa aaagcagaac aaggtctcca gagaaaaaaa tcaaaagaat3420ggttgagaaa tctcttccat ccaaaatgac taataagact acaagtaaag aagtatctga3480aaatgaaaaa ggaaagaaag taaccacagg agaaacaagt tctagtaacg ataaaatagg3540agaaaatgtc cagctatcag aaaagaggct gaagcaagaa cctgaagaga aggtagtttc3600aaacaaaaca gaagatcaca aagggaaaga actagaggca gctgacaaaa acaaagagac3660tggaaggttc aaaccggaaa gcagttctcc agttaagaaa acactgataa ctccagggcc3720ctggaaggtt ccaagtggaa ataaagtcac aggcactatt ggtatggctg agaaacggca3780gtaaccttta gtataaaaca aagaaaaaca agttgtaatc ttttcttaca gcagcatttt3840tccagaaaaa gccttttttt ttttttcaga tattctgaaa cagataagta catgttaatg3900tgagcctcaa gttacctagg ctgcatgaag ggcctttagg attgctaaga accaactgtc3960cccctggccg gctgccctcc ctcgctctca ggaaggagct gcatccacat gctcatctga4020cccgccctgc tcaggctgcc cagctcgtct tcatgagtgt ctgaacaaat gacatatgtt4080gatattaaca atgtggtcac aactcacttt gtatttgtgc caagttatct actgtatcat4140gtctgttttt atcctttttg ttcagctgtt tccacagtaa tgaaaaagtt aggtttggct4200tggaagttga tgatctcaat agcatgttgc atgtttacag agagaaatat gtgagtcctt4260gcagaagaag agactgttaa ctcatcgtta aagatggccg ttgtctcttc taacagctac4320tgatgatgtc ccactttaaa aataaaaccc ccaaacatca ctactttaag gaaaaaaaaa4380atgtagtcca atattgatgc tttcttatgg ctttttattt taatttggct ggataagttg4440tttcaaataa ctgttaaaga tattacttac aattgaatgt ttgaaataag aaagtacttt4500aagcaataga gttcatctcc tgctgtgtta tccaacctcg atgtatactt acagcatctc4560aggtcaccct ttttatttca gttatttaat tatgaaacca taaagaagca tgtggaaata4620gtgtttattg ctctttgaag aaaaaccacc aactatttct ggatattttg gctgtaccta4680ctactaaagt cattagtctt taatacataa tacatatttg aaaagtaaac atattatata4740gattatgtga gggacttaat catgaaacca gtttcacagt ccaagtacca actcttctgg4800tagcaggtgc acaagcttgg gtgtttaaaa acaacctgtg tagggtatgc ccagcaaatg4860aggacaaatg tgtagacagt acttactgga tcttatttaa cttttagcta cattaactaa4920ctttcttatt taaaaacaag aaagggagac taaacatctg cttaacttgt acacattttc4980agaattcttt ttaaaagtct agttaaagat gtttcttaga agttggagac tgttaacaac5040ttccataaaa tagatccagg tttttcagtt ccctgaagca gcattcagta gcatctatat5100aaataaaggc accttctgag aataaaacta ttttatggag tgtgtgaaca cacttgttct5160
gtcacctggg ttcatcttgt tgtgaagcac attaggtcca ggtccttccc tctgggagtc5220tgactgtgaa actctttaac ccaacaactc aattagcccc tgtagataag acatgcttcc5280cagagtgaga tttttgaaat ccccttttca tccagaacta tatttaccca cctattgtaa5340ctattcaaat agagcaaaat taggaggctt gataaatact aagaatttag taccacagaa5400attatttatt attttccctg tagtccacaa ttagtgataa cgaatcctat ttttgttaac5460tgtgacataa ctttgatgtc atatgttgtc ctatgtggtt cttcctaagt aaactctgta5520ctgattatat actgacttag caatgtggcc ttggaatgct gagcaaaatg tggatgtact5580ggttgtaaat gtttatatat tgtacagtac ctttatatat acacttgagg ttctgattag5640<210>10<211>457<212>DNA<213>人<220>
<221>misc_特征<222>(242)..(242)<223>任意堿基<220>
<221>misc_特征<223>任意堿基<220>
<221>misc_特征<222>(394)..(394)<223>任意堿基<220>
<221>misc_特征<222>(406)..(406)<223>任意堿基<220>
<221>misc_特征<222>(457)..(457)<223>任意堿基<400>10tcagtcactc tttcaccctg ccaaagcttc actgtcctac tgattgaatt gtatgtgaga 60aataaaatgt catcatatta agccactggg atttgtatgt ttatctgtta tagcagcaag 120tcttaattta cctaatacac acattgtgac agatgttctt aatgtcccac cccatattgt 180tacatgtcca gctttgagga tccctggcat gtgggggtag gagtttctgg gcatgctgga 240tncaattccc acttttaagg catctgtggc ctctgtggcc tctgtggcct tcactgttat 300ggaagggatt tatctggggc accataggaa actttaccat ggcacagtgg acaacctagg 360agggggtgng gaggaggggc cttcaggccc aacngggggg accagngttc gtggggttag 420ggtggtttgg ggggttttcc ctcttacccg tgggggn 457
<210>11<211>1493<212>DNA<213>人<400>11aatagggttg gcggctgcag cgggcggcaa acagcccgcc cggcaccacc atgctcgccc 60tggaggctgc acagctcgac gggccacact tcagctgtct gtacccagat ggcgtcttct 120atgacctgga cagctgcaag cattccagct accctgattc agagggggct cctgactccc 180tgtgggactg gactgtggcc ccacctgtcc cagccacccc ctatgaagcc ttcgacccgg 240cagcagccgc ttttagccac ccccaggctg cccagctctg ctacgaaccc cccacctaca 300gccctgcagg gaacctcgaa ctggccccca gcctggaggc cccggggcct ggcctccccg 360cataccccac ggagaacttc gctagccaga ccctggttcc cccggcatat gccccgtacc 420ccagccctgt gctatcagag gaggaagact taccgttgga cagccctgcc ctggaggtct 480cggacagcga gtcggatgag gccctcgtgg ctggccccga ggggaaggga tccgaggcag 540ggactcgcaa gaagctgcgc ctgtaccagt tcctgctggg gctactgacg cgcggggaca 600tgcgtgagtg cgtgtggtgg gtggagccag gcgccggcgt cttccagttc tcctccaagc 660acaaggaact cctggcgcgc cgctggggcc agcagaaggg gaaccgcaag cgcatgacct 720accagaagct ggcgcgcgcc ctccgaaact acgccaagac cggcgagatc cgcaaggtca 780agcgcaagct cacctaccag ttcgacagcg cgctgctgcc tgcagtccgc cgggcctgag 840cacacccgag gctcccacct gcggagccgc tgggggacct cacgtcccag ccaggatccc 900cctggaagaa aaagggcgtc cccacactct aggtgatagg acttacgcat ccccaccttt 960tggggtaagg ggagtgctgc cctgccataa tccccaagcc cagcccgggc ctgtctggga1020ttccccactt gtgcctgggg tccctctggg atttctttgt catgtacaga ctccctggga1080tcctcatgtt ttgggtgaca ggacctatgg accactatac tcggggaggc agggtagcag1140tgcttccaga gtcccaagag cttctctggg attttcttgt gatatctgat tccccagtga1200ggcctgggac ctttttaaga tcgctgtgtg tctgtaaacc ctgaatctca tctggggtgg1260gggccctgct ggcaaccctg agccctgtcc aaggttccct cttgtcagat ctgagatttc1320ctagttatgt ctggggccct ctgggagctg ttatcatctc agatctcttc gcccatctat1380ggctgtgttg tcacatctgt cccctcattt ttgagatccc ccaattctct ggaactattc1440tgctgcccct ttttatgtgt ctggagttcc ccaatcacat ctagggctcc tcc 1493<210>12<211>2292<212>DNA<213>人<400>12ccatgggttc cccttcagcc tgtccataca gagtgtgcat tccctggcag gggctcctgc 60tcacagcctc gcttttaacc ttctggaacc tgccaaacag tgcccagacc aatattgatg 120
tcgtgccgtt caatgtcgca gaagggaagg aggtccttct agtagtccat aatgagtccc 180agaatcttta tggctacaac tggtacaaag gggaaagggt gcatgccaac tatcgaatta 240taggatatgt aaaaaatata agtcaagaaa atgccccagg gcccgcacac aacggtcgag 300agacaatata ccccaatgga accctgctga tccagaacgt tacccacaat gacgcaggat 360tctataccct acacgttata aaagaaaatc ttgtgaatga agaagtaacc agacaattct 420acgtattctc ggagccaccc aagccctcca tcaccagcaa caacttcaat ccggtggaga 480acaaagatat tgtggtttta acctgtcaac ctgagactca gaacacaacc tacctgtggt 540gggtaaacaa tcagagcctc ctggtcagtc ccaggctgct gctctccact gacaacagga 600ccctcgttct actcagcgcc acaaagaatg acataggacc ctatgaatgt gaaatacaga 660acccagtggg tgccagccgc agtgacccag tcaccctgaa tgtccgctat gagtcagtac 720aagcaagttc acctgacctc tcagctggga ccgctgtcag catcatgatt ggagtactgg 780ctgggatggc tctgatatag cagccttggt gtagtttctg catttcggga agagtgtttt 840tattatccac ctgcagactg gactggattc ttctagctcc ttcaatccca ttttctcctg 900tggcatcact aagtataaga cctgctctct tcctgaagac ctataagctg gaggtggaca 960actcaatgta aatttcaagg aaaaaccctc atgcctgaga tgtgggccac tcagagctaa1020ccaaaatgtt caacaccata actagagaca ctcaaattgc caaccaggac aagaagttga1080tgacttcatg ctgtggacag tttttcccaa gatgtcccaa gcctcatcgt gacgaggctc1140ttatcccact ccatttttcc ctgctcatgc ctgcctcttt aatttggtaa gataatgctg1200taactagaat ttcacaatca gcgccttgtg caggcaattt gacagagtgt tggatgtgtc1260atgtcatcat gtcaaaccca aatatttgac ctaagggatc ctttattctg cccagtggct1320aactttaaca acatccctaa tacaactgtt tattcaaatg cacggtggtc cctgttagag1380ttagacctct agactcacct gttctcacgc cctgttttaa tttaacccag ctatgggatg1440ccagataaca gaattgctgc ctacgagctg aacagggagg agtttgtgca gttgctgaca1500cttcttgttg cacataaata aatacagtgg gtactataga gactcagttg caaaaattaa1560caaatatgct gcttgattaa aatgggtagg cttctcatgt ggctcattct ttaatctatt1620ctcttttatt tggtttggtt catggggtct ctgcctatgg atcatacttc aaactcttgg1680tgtgatcctc ctgattgtca caatattagt taccctggtg tgctgtattc tctaaaacct1740ttaaatgttt gcatgcagcc attcgtcaaa tgtcaaatat tctctctttg gctggaatga1800caaaaactca aataaatgta tgattaggag gacatcataa cctatgaatg atggaagtcc1860aaaatgatgg taactgacag tagtgttaat gccttatgtt tagtcaaact ctcatttagg1920tgacagcctg gtgactccag aatggagcca gtcatgctaa atgccatata ctcacactga1980aacatgagga agcaggtaga tcccagaaca gacaaaactt tcctaaaaac atgagagtcc2040aggctgtctg agtcagcaca gtaagaaagt cctttctgct ttaactctta gaaaaaagta2100atatgaagta ttctgaaatt aaccaatcag tttatttaaa tcaatttatt tatattcttc2160tgttcctgga ttcccatttt acaaaaccca ctgttctact gttgtattgc ccagtaggag2220
ctatcactat attttgcaga atggaaactg ccctgactct tgaatcacaa ataaaagcca2280attgtatctg tt2292<210>13<211>519<212>DNA<213>人<220>
<221>misc_特征<222>(212)..(212)<223>任意堿基<220>
<221>misc_特征<222>(451)..(451)<223>任意堿基<400>13gaaacaacaa cagtgtaatc tttaacaggg atgttaaagg taagaagtca ggaagataaa 60ccaaaatgat tgagtatgat aaagaatttt gcatggcgat taaaatagaa aacctataaa 120tgtagaaaaa gcaggtctgg acttagcaaa gaaacaatat agtttggaga aggcatgaaa 180taagttcttt tcatgttcac tgctggtcac ancataacag agagtgatgt ggagagcttt 240gggaaggttt cacgttgagt tacatcagtg gtcaacaatg gagcaacaag actccgtaga 300ggatgccacc ctgggagaat tgcaagggaa aggaggctga agcacaactg gtaatagcct 360tcagatattt aatggatatg caaataaagc tctgattaat tgtattttca cttattatat 420atcatctttg gacctttcta aaagtgggac nctagaaaag atatactgaa actccaaaag 480aatacttcag ctcgagttga atggattcaa gatgttgtt519<210>14<211>5294<212>DNA<213>人<400>14ggctcgcatc cccatagtgc tgggttacag tgaaggtacg ccccgcgctc tgctctggag 60aggcagggtg ggatagggaa cgtctcgagt ggcgcccgca gtcatggtgg tgttcgttgg 120ccgccgcctc ccggcgctcc tagggctgtt taagaagaag ggctctgcca aggctgagaa 180tgacaaacat ctaagtgtag ggcctggcca ggggccaggg tctgcagtgg atgagcacca 240ggacaacgtc ttctttccca gtgggcgacc cccccacctg gaagagctgc acactcaggc 300ccaggagggg ctccgctccc tacaacacca agagaaacag aaactgaaca agggtggctg 360ggaccatgga gacacccaga gtatccagtc ctcccggacg gggccggatg aagacaacat 420ctccttctgc agtcagacca catcctacgt ggctgagagc tccacagcag aggacgcgct 480ctccatccgc tcggagatga tccagcgcaa aggctccacc ttccgacccc atgactcatt 540tcccaaatct ggaaagtcag ggcggcgtcg gcgggagcgg cggagcactg tgctgggact 600
cccgcagcat gtgcagaagg agcttggcct gaggaatgag cgtgaggcac caggcacgcc 660ccgggctcct ggtgcacggg atgccgtacg catccccaca gtggacggcc gcccccgagg 720cacctcaggg atgggggccc gggtgtccct gcaggcgctg gaggcggagg cagaggctgg 780cgctgagaca gaggccatgc tgcagcgcca cattgaccgt gtctaccggg atgacacctt 840tgttggccgg tccacgggta cccgggcccc accattgacc cggcccatgt ccctagcagt 900gcctggattg acaggagggg cagggcctgc agagcccctg agcccggcca tgtccatctc 960cccccaggcc acctacctgt cgaagttgat tccacatgct gtgctgccgc ctacagtgga1020cgtggtggcc ctaggccgct gcagcctgcg cacactaagc cgctgcagcc tgcactcggc1080cagcccagcc tcagtccgct cgctggggcg cttctcctcc gtctccagcc cacagccccg1140cagccgccac ccatcctcct ccagtgacac ctggagccac tctcaatcct ccgacaccat1200tgtgtctgac ggttccaccc tctcctctaa gggtggctct gagggccagc cggagagctc1260tacggctagc aatagcgtgg taccccctcc ccagggaggc agtgggaggg gctctcccag1320tgggggcagc actgctgagg cctcagacac actcagcatt cggagcagtg ggcagttgtc1380tggccggagt gtgtccctgc gtaagctgaa gcggcctcca ccccctcccc gccggaccca1440ctccctccat cagcggggct tagcagtgcc tgatgggcca ttagggttgc cccctaagcc1500tgagcgtaag cagcagcccc agctgcctcg gccacccacc actggtggct cagaaggggc1560gggggcagca ccctgtccac ccaacccagc caacagctgg gtacctggct tgtctccggg1620tggttcccgg cgccccccac ggtccccaga acggacactt tcgccctcca gtggatactc1680gagccaaagt ggtactccca ccctccctcc caagggcctg gcaggtcccc ctgcttcccc1740aggcaaggcc cagcccccta aaccagagcg tgtcacgtct cttcgctccc ctggggcctc1800cgtctcctct tccctcacgt ctttatgttc ctcctcctct gacccagccc cctcagaccg1860ctctgggcca cagatattga cccccctggg tgacaggttt gtcatacctc ctcaccccaa1920ggtgcctgcc cccttctccc cacctccctc caagcccagg agccctaacc cagctgcccc1980tgctctagcc gcccctgctg tggttcctgg gcctgtttct accactgacg ccagtcctca2040gtcccctccc actccccaga caaccttgac tccactgcag gagtctcctg tcatctccaa2100agaccagtca cccccacctt ccccaccccc atcttatcat ccacccccac cacccactaa2160gaagccagag gtggttgtgg aggcaccatc tgcctcagag actgctgagg agcccctcca2220agatcccaac tggccccctc ccccaccccc tgcccctgag gagcaggacc tgtccatggc2280tgacttcccc ccaccagagg aggctttttt ctctgtggcc agccctgagc ctgcaggccc2340ttcaggctcc ccagagcttg tcagctcccc ggctgcttcg tcctcctcag ctactgcttt2400gcagattcag cccccgggta gcccagaccc tcctccagct ccgccagccc cagctcctgc2460tagttccgcc ccagggcatg tggccaagct ccctcagaag gaaccggtgg gctgtagcaa2520gggtggtggg cctcccaggg aggacgtagg tgcgcccctg gtcacgccct cgctcctgca2580gatggtgcgg ctgcgctccg tgggtgctcc aggaggggct cccaccccag cactggggcc2640atcggccccc cagaaaccac tgcgaagggc cctgtcaggg cgggccagcc cagtgcctgc2700
cccctcctca gggctccatg ctgcggtccg actcaaggcc tgcagcctgg ccgccagtga2760aggcctctca agtgctcagc ccaacggacc gcctgaggca gagccacggc ctccccagtc2820ccctgcctca acggccagtt tcatcttctc caagggctct aggaagctgc agctggagcg2880gcccgtgtcc cctgagaccc aggctgacct ccagcggaat ctggtggcag aactccggag2940catctcagag cagcggccac cccaggcccc aaagaagtca cctaaggctc ccccacctgt3000ggcccgcaag ccgtctgtgg gagtcccccc acccgcctcc cccagttacc ctcgagctga3060gccccttact gctcctccca ccaatgggct ccctcacacc caggacagga ctaagaggga3120gctggcggag aatggaggtg tcctgcagct ggtgggccca gaggagaaga tgggcctccc3180gggctcagac tcacagaaag agctggcctg accaccaggc acctcactgg cactgctgac3240ccatcccaga aacacaatct cagggacccg agcagctcca aggacgagag gatacagcag3300acacaaccta atagagaggg cgcctgcagc cttaacctcc acggccttcg atacttatgc3360aagcctggtg ttgctcctgt cctcagagtc atcctgcgct catgcctttt cccgaatggg3420ttcacctctg gcagttgccg cttcagtctt ggccttagcc tcatcttgaa gtgggtagct3480ggcgggagag ggtggctgcg ccccctgctg gccctgaggc tgcagagttg ggagcaggac3540acctcacctg agtttcattt tttttcatgt ccaaaccatg cacatactat agtccagaat3600caaagcactt ttgaaaagtg gctgcatggc catcctccag ggcccaggaa gttgcattcc3660aagggcctgt ttacatggca gcagaatcca tccccggcag tcagcccata gcttgggacc3720agtctgtgcc ctcctgccca gtccagttta ctcctcttgg ttcctgaagg tggccaagtc3780attgtgttcc cacaggcttc tctaggctgg gggcaggtgt ggggctgtgg aattccaaag3840cacaaaaggt gcagagggga ttggccttcc tgtgcctcaa ctcaccaacc accctcctgc3900cttccagttc tgccaggtgc tccatgctgg ggacaagtag gagactgcca gggcccaaag3960aaatgggtga gcagtagagt catctcgggg cacttggcag tgtcaagcac ctgccccttg4020cctccttgac cacactgggg tgggtgggcc cccagcactt cagaggcagg agcctttggg4080ctgagcaagc actgaggagg tggatggaag ggagcatctg gaggggggga gcttccttga4140gcagtgggcc caggcctggc cctccacact tcattctctg acctttctct ctcctcattt4200cggtgcatgt cctttctgca gctgcctttc agcacaggtg gttccactgg gggcagctaa4260cgctgagtga caaggatggg aagccacagg tgcattttac tcaagtcttc tctagtcaat4320gaggggcacc cagtgcttct agggcaggct gggtggtggt cccctaggta tcagcctctc4380ttactgtact ctccgggaat gttaaccttt ctattttcag cctgtgccac ctgtctaggc4440aagctggctt ccccattggc ccctgtgggt ccacagcagc gtggctgccc cccagggcca4500ccgcttcttt cttgatcctc tttccttaac agtgacttgg gcttgagtct ggcaaggaac4560cttgctttta gcttcaccac caaggagaga ggttgacatg acctccccgc cccctcacca4620aggctgggaa cagaggggat gtggtgagag ccaggttcct ctggccctct ccagggtgtt4680ttccactagt cactactgtc ttctccttgt agctaatcaa tcaatattct tcccttgcct4740
gtgggcagtg gagagtgctg ctgggtgtac gctgcacctg cccactgagt tggggaaaga4800ggataatcag tgagcactgt tctgctcaga gctcctgatc taccccaccc cctaggatcc4860aggactgggt caaagctgca tgaaaccagg ccctggcagc aacctgggaa tggctggagg4920tgggagagaa cctgacttct ctttccctct ccctcctcca acattactgg aactctatcc4980tgttaggatc ttctgagctt gtttccctgc tgggtgggac agaggacaaa ggagaaggga5040gggtctagaa gaggcagccc ttctttgtcc tctggggtaa atgagcttga cctagagtaa5100atggagagac caaaagcctc tgatttttaa tttccataaa atgttagaag tatatatata5160catatatata tttctttaaa tttttgagtc tttgatatgt ctaaaaatcc attccctctg5220ccctgaagcc tgagtgagac acatgaagaa aactgtgttt catttaaaga tgttaattaa5280atgattgaaa cttg 5294<210>15<211>988<212>DNA<213>人<400>15gtcgtgaggc gggccttcgg gctggctcgc cgtcggctgc cggggggttg gcctgggtgt 60cattggctct gggaagcggc agcagaggca gggaccactc ggggtctggt gtcggcacag 120ccatggcggg cgcgttggtg cggaaagcgg cggactatgt ccgaagcaag gatttccggg 180actacctcat gagtacgcac ttctggggcc cagtagccaa ctggggtctt cccattgctg 240ccatcaatga tatgaaaaag tctccagaga ttatcagtgg gcggatgaca tttgccctct 300gttgctattc tttgacattc atgagatttg cctacaaggt acagcctcgg aactggcttc 360tgtttgcatg ccacgcaaca aatgaagtag cccagctcat ccagggaggg cggcttatca 420aacacgagat gactaaaacg gcatctgcat aacaatggga aaaggaagaa caaggtcttg 480aagggacagc attgccagct gctgctgagt cacagatttc attataaata gcctccctaa 540ggaaaataca ctgaatgcta tttttactaa ccattctatt tttatagaaa tagctgagag 600tttctaaacc aactctctgc tgccttacaa gtattaaata ttttacttct ttccataaag 660agtagctcaa aatatgcaat taatttaata atttctgatg atgttttatc tgcagtaata 720tgtatatcat ctattagaat ttacttaatg aaaaactgaa gagaacaaaa tttgtaacca 780ctagcactta agtactcctg attcttaaca ttgtctttaa tgaccacaag acaaccaaca 840gctggccacg tacttaaaat tttgtcccca ctgtttaaaa atgttacctg tgtatttcca 900tgcagtgtat atattgagat gctgtaactt aatggcaata aatgatttaa atatttgtta 960aaaaaaaaaa aaaaaaaaaa aaaaaaaa988<210>16<211>4908<212>DNA<213>人<400>16ggataacctc gcagggtggg ccggagggcg ggcgccgccg ctgcctgtgc tgcggcgatg60
gcccagtgtg tacaatcagt gcaggagcta atcccggact ccttcgtccc ctgtgtcgct 120gcgctgtgca gcgacgaagc cgagcggctc actcgtctca atcacctcag cttcgcggag 180ctgcttaagc ccttctcccg cctcacttcc gaggttcaca tgagagatcc taataatcaa 240cttcacgtaa ttaaaaattt gaagatagca gtaagcaaca ttgtcaccca gccacctcag 300cctggagcca tccggaagct tttgaatgat gttgtttctg gcagtcagcc tgcagaagga 360ttagtagcta atgtgattac agcaggagat tatgacctta acatcagtgc cactactcca 420tggtttgagt cttacagaga aacctttctt cagtcgatgc cagcatcgga tcatgaattt 480ctgaaccact atttagcatg tatgttggta gcgtcatcta gtgaagctga acctgtggaa 540cagttttcaa agttgtcaca agaacagcat cgaattcagc acaacagtga ttattcctac 600cccaagtggt ttataccaaa tacacttaaa tactatgtac ttttacatga tgtaagtgca 660ggagatgaac agagagctga atcaatttat gaagaaatga aacagaaata tggaactcag 720ggttgctatt tacttaaaat taattctcga acatctaatc gagcatcaga tgaacagata 780ccagatcctt ggagtcagta tctccagaaa aatagtattc aaaaccagga atcatatgaa 840gatggccctt gtactataac ttcaaataag aattctgata ataacttgct ttcattggat 900ggattagata acgaagtcaa agatggctta ccaaataact ttagagctca cccacttcag 960ttggagcaat ccagtgaccc ttctaacagt attgatggcc cagatcatct aagatctgct1020tcatcgttac atgaaacaaa gaaaggaaat actggaataa ttcatggtgc atgtttaaca1080cttactgatc atgatagaat tcgacagttt atacaagagt tcacatttcg gggccttttg1140ccacatatag agaaaacaat taggcaatta aacgatcagc taatatcaag aaaaggtttg1200agtcgatctc tattttctgc aactaaaaaa tggtttagtg gcagtaaagt tccagaaaag1260agcattaatg acctgaaaaa tacatctggc ttgctgtatc ctccggaagc accagaactt1320caaatcagga aaatggctga cttatgtttt ttggtgcagc attatgattt ggcttacagt1380tgctatcata ctgcaaagaa agattttctt aatgatcaag caatgcttta tgcagctggt1440gccttggaaa tggcagcagt gtctgctttt cttcaaccag gagcacctag gccatatcct1500gctcattaca tggatacagc aattcagaca tacagagata tctgcaagaa tatggtgttg1560gctgaaagat gtgtgttgct tagtgctgaa cttttaaaaa gccaaagcaa atattcagag1620gctgcagctc tcctaatacg gttgaccagt gaggattctg atcttcgaag tgcacttctt1680ttggaacagg cagcacattg ctttataaac atgaaaagtc ccatggttag aaaatatgca1740tttcatatga tattggcagg ccatcgattt agtaaagcag ggcagaaaaa gcatgcttta1800cgctgttatt gtcaagccat gcaagtttac aaaggaaaag gctggtctct tgcagaggat1860cacattaatt tcactattgg gcgccagtcc tatactctta gacagctgga taatgctgtg1920tctgctttta ggcatattct aattaatgaa agtaaacaat ctgctgctca acagggggct1980ttcctcagag aatatcttta tgtttacaag aatgtaagtc agctgtcacc agatggtcct2040ttgccacagc ttcctttacc gtatattaac agttcagcaa cacgggtttt ttttggccat2100
gacagacgac cagcggatgg tgaaaaacaa gcagctactc atgtaagtct tgatcaagaa2160tatgattctg aatcctctca gcagtggcga gaacttgagg aacaagttgt ttctgtggtt2220aacaaaggag taattccatc caattttcat cccacacaat actgtttgaa cagttactca2280gataattcaa gatttccact tgcagttgta gaagaaccaa ttacagtgga agtggctttt2340agaaaccctt tgaaagttct acttttgttg actgatttgt cattgctttg gaagtttcat2400cctaaagatt tcagtggaaa ggataatgaa gaagttaaac aactagttac aagtgaacct2460gaaatgattg gagctgaagt tatttcagag ttcttaatta atggcgaaga atcaaaagtg2520gcaagactaa agctctttcc ccatcacata ggggagctgc atattctggg agttgtttat2580aatcttggca ctattcaggg ctctatgaca gtagatggca ttggtgctct tcccggatgt2640cacacaggaa aatattcctt gagtatgtca gtccgaggga agcaggattt agaaattcaa2700ggtcctcgac ttaacaacac aaaagaagag aaaacatctg ttaaatatgg ccctgatcga2760cgtttagatc ccataatcac agaagaaatg ccactgttgg aggtgttctt tatacatttt2820cctacagggc ttctctgtgg agaaatccga aaagcatatg tagaatttgt caatgtcagc2880aaatgtccac ttactggatt gaaggttgtt tctaaacgtc cagagttctt tactttcggt2940ggtaatactg ctgttctaac accactaagt ccctcagctt ctgagaattg tagtgcttac3000aagactgttg tgacagatgc tacctctgtg tgtacagcac tcatatcatc agcttcttct3060gtagactttg gcattggcac aggaagtcaa ccagaggtga ttcctgttcc ccttcctgac3120actgttcttc tacccggagc ctcagtgcag ctgccaatgt ggttacgtgg gcctgatgaa3180gaaggtgtcc atgaaattaa ctttttgttt tactatgaaa gtgtcaaaaa gcagccaaaa3240atacggcaca gaatattaag acacactgca attatttgta ccagtcggtc tttaaatgta3300cgggccactg tctgcagaag taattctctt gaaaatgaag aaggcagagg aggcaatatg3360ctagtctttg tggatgtgga aaataccaat actagtgaag caggcgttaa ggaattccac3420atagtgcaag tatcaagtag tagcaaacac tggaagttac agaaatctgt aaatctttct3480gaaaacaaag atgccaaact tgccagtagg gagaagggaa agttttgctt taaggcaata3540agatgtgaga aagaagaagc ggccacacag tcctctgaaa aatatacctt tgcagatatc3600atctttggaa atgaacagat aataagttca gcaagcccat gtgcagactt cttttatcga3660agtttatctt ctgaattgaa aaaaccacaa gctcacttgc ctgtgcatac agaaaaacag3720tcaacagagg atgctgtgag attgattcaa aaatgcagtg aggtagattt gaatattgtc3780atattatgga aggcatacgt tgtggaagac agtaaacagc ttattttgga aggtcaacat3840catgttattc ttcgcactat aggaaaagaa gccttttcat atcctcagaa acaggagcca3900ccagaaatgg aactattgaa atttttcagg ccagaaaaca ttacagtttc ctcaaggcca3960tcagtagagc agctttctag tctcattaaa acgagtcttc actacccaga atcatttaat4020catccatttc atcaaaaaag cctttgttta gtaccagtca ctcttttact ttccaattgt4080tctaaggctg atgtagatgt catagttgat cttcggcata aaacaacaag tccagaagca4140ctggaaatcc atggatcatt cacatggctt ggacaaacac agtataaact tcaacttaaa4200
agccaggaga ttcacagtct gcagctgaaa gcatgctttg ttcatacagg tgtttataac4260cttggaactc ctagggtatt tgccaagtta tcggaccaag ttacagtgtt tgaaacaagt4320cagcagaatt ccatgcctgc cctgatcatc atcagtaatg tgtgacaact tggaaatttg4380tactgaaatc cacaataatc agtttttgct ggatgggttt tacagcagta tttgatatac4440ctaacttgtt atggaggttg attgatatct gatccctgca aaatactttg acttgtcatt4500ttgttgatga tgcaaagcac gttggactga gaatacttaa cattcttttt ctgtatttct4560ttaaaccctg agaataattt acatgctcat aatacaggat atcagcatat ttgtgcacct4620tattaagccc catcttaaga aaacacaaag tctaagtctg ctgttacaac ttgtcaatgg4680tatacgaata ttaggagatg attctgagaa aggaaaggcc ttgttggcag tactcctgtt4740aagccattag tctctaaatt ccagctttac tgtgaagttc tatagagtgt taaatacaaa4800ttttcctgtc ttgcttcaca cagttcctta aaatcagttt tgaactttgg tcatagagtc4860ttcatatttc agtatttggt ggtccctatg acttatacat aactttgt 4908<210>17<211>435<212>DNA<213>人<220>
<221>misc_特征<222>(30)..(30)<223>任意堿基<220>
<221>misc_特征<222>(49)..(49)<223>任意堿基<220>
<221>misc_特征<222>(75)..(75)<223>任意堿基<220>
<221>misc_特征<222>(76)..(76)<223>任意堿基<220>
<221>misc_特征<222>(78)..(78)<223>任意堿基<220>
<221>misc_特征<222>(79)..(79)<223>任意堿基<220>
<221>misc_特征
<222>(109)..(109)<223>任意堿基<220>
<221>misc_特征<222>(136)..(136)<223>任意堿基<220>
<221>misc_特征<222>(137)..(137)<223>任意堿基<220>
<221>miac_特征<222>(149)..(149)<223>任意堿基<220>
<221>misc_特征<222>(227)..(227)<223>任意堿基<220>
<221>misc_特征<222>(236)..(236)<223>任意堿基<220>
<221>misc_特征<223>任意堿基<220>
<221>misc_特征<222>(342)..(342)<223>任意堿基<220>
<221>misc_特征<222>(363)..(363)<223>任意堿基<220>
<221>miac_特征<222>(389)..(389)<223>任意堿基<220>
<221>misc_特征<223>任意堿基<400>17ggtagaaatg attgtgatgt acaaattttn tattttgatc atacttaana agacagagca 60gactcacatt cattnncnna atagtatcac tgtacacata gcgaatttnt ggcgctttta 120
gattgctctg aaaatnnctg aagagttgnc catagcagcc tggtaagcct tttcctttcc 180cccaaagctc tcctgccctt tgcagaaaga ctgttggtga caactgntgc taactnaata 240gcatgnggtt gaacttcgcc aaaatccttc cacctcctcc catagggcaa caggggtgac 300ttgggcttaa agggcattga gtaagcaagt aggttatcag anaacagagg gaagattcca 360ttntagataa tttccaaata ttacaattng tggaactcag agttcaactg ctcagttcct 420tcttcngctg accct 435<210>18<211>2224<212>DNA<213>人ctttagatct gtgcagcctt tgcgtgccaa acttgtgaaa ttccttttac cttttttgga 60gtacttgcta taaagccacc tgtcaacaaa cccccattat gtacagaata ggacctatcc 120agtagccagg ccagtaggca gttggggaag gtgggaagga tccagcgagg cccctgagcc 180tgcacctgga caggtgtacg tctgcaccca tcaccctcag caccaggcca ccctgcagtc 240cacttactgt actgtgttgt ggaaggatat gctaagtgat gaaagttgcg agcagtctca 300ctggtcgtgt aaactttttt ttttttttgg aaattgaagc tgtagagtgc tgcccgaaat 360ctctaggaag ttggtggcaa gggacagcac tcacactctt ctggtcatga tctctgatct 420ccacctcaaa tgacaataaa aaactggtcc aacgaagaca ctgctcagca cttcagccat 480caggactaat ccatcgatga ctggaaaaga ggctagcttt gaggaaaaca gcctgggctc 540ttgggagcag agtccagtgg gtgtgaggct gacttgccga cggtcggcag gtaatggctc 600tcagccggcg aggcggtccc acagctctcc tcccagggca gcctgaggag gaggaggccg 660ggtgcctgtt tggtggcagc ttcagcctag ggatacctga agctgttgag caacaccttt 720atgaaatgtt gccagagcag caacacttcc ctgtgggcac agccccggga aatccggtac 780caagtgagca aggtggcagg acccacccaa gcctgatacg catctgggcc cgccgggctc 840agcaggggag gctgctacgg ctgcccactt cccagcaccg tctgtcaggc ttgaacccct 900ctgtgctgtt cccttcctgg ctaataggga gacccttcgc aggcacccac tgtttcaact 960tgaccctccc accccctgct actctcctcc acacacccct ccgttccgct agcctaccct1020gtcagccttt caataaaagt tatgcacaaa tgtgaacacc tgagatggag ctgaacattt1080cttcactttg ttctttttct gaagtcaaac tcttatcaaa tgccctaaaa ttattaccac1140ccaagagaaa caggaaaaag gttacatgtt tttgtttact gagagtaaga tcacctgcat1200ctggaagacg ggctggtaaa ttggtttggc tacagaacag aaagaaaaca aaaacaaacc1260tcgtaaggga agtatcgcac tcagacacca ccacttccta gagccaaatg agcaatccca1320aactgcaagt gccgtaagtg ggcctgtgac gtcacaccgc ccggcccgag gtatcgcatg1380tgcgggggag gcccacacta cagctgtcct ctcgtctaga aggcaccacc tcgctttcat1440gtcccgtgtg ttttggaaaa gcagtatggt gtgtcatgtc tagcggcgaa cacttccctc1500
cctctgtcct tgaggttgta atataaaaac tgtgtttctg tacgtgtggg tgggaattct1560ctgacggtgc tcgttcatag cacaagctta cgctgagttc tgaactgtcg ttcacagctg1620cgtgtctgca tggtgtcgca tctgttgtac ctttggggaa aatttgtatg taaatgtaca1680gaaataaaaa cgttgcccca ttaacagatt tcctctggaa tgtcttccct acctcacctg1740atggtatcca ccgaagggca tttcactacc attaatggtg agtaataaaa tcctccgtgt1800tcattcagac ctcactgcgt cactactttg aacgcctctg taagctgtgt cttcacccgc1860cccgaggtgg gtggagggag gcctctcact ctgcttcgag tcctggtctt aaaggtagtc1920agaggcagag gctggattaa acacacactg tttaccaagt gccactctca gaccacctga1980gagacggggg gccatcagta aaattaagag gaattttttt cccttgttcg tgtatgttct2040gctgatccgt ggcctgaagg ttcctagaga cgtcaagaaa tgaatatctt acactgtgat2100tctgtgagga aagactggta acccaaaact ctcttctcta atgtattttt taacgaaaat2160gacaatattt ctttaataaa gtatttatac caaaaaaaaa aaaaaaaaaa aaaaaaaaaa2220aaaa 2224<210>19<211>2244<212>DNA<213>人<400>19gttgtttaaa agcaaggcat gcttgtggat gactctgtaa cagactaatt ggaattgttg 60aagctgctcc ctggttccac tctggagagt aatctgggac atcttagtgt tttgttttgt 120ttttttccct cctctttttt tgggggggag tgtgtgtggg gtttgttttt tagtcttgtt 180tttttaattc attaaccagt ggttagcctt aaggggagga ggacggattg attccacatt 240ccacttccta gatctagttt agaaaacatg ttccccatct ggtgctctta ggaaggagta 300tagtaaatgc ctcatttaat aacatactcc tttttgaaag ttgccttttc tctccaccct 360tgagtagatc cagtatttga tgaaactcat gaaagtgggt ggagcccatc ttccccctcc 420tcttttctag gacgcactat atgtgactgt gactttaagg acatttgttt gccatttgct 480gatttttttg ggaagttaat ttctaacttc tttcactgat aaatgaagaa aagtattgca 540cctttgaaat gcaccaaatg aattgagttt gtaattaaaa aaattttttt tccctttcag 600tcattgtctt atatgcttag catagatttg cagctcagta gtatatgtgt tcctagaatg 660cagctgaaga cctgttatgt agaggaaata cgaggggtgg tgctagaaga cagacatctg 720tggaatgatt cacatcctct caagttagga ggatggaggc ctgcttcatt aagaagctgg 780gggtagggtg ggggtgggga gaacacttaa caacatgggg accagtcagg ggaatcccct 840tatttctgtt ttgcatatga ggaaccctag agcagccagg tgaggctctc tagtttaata 900aaaatcatgg aaagactctt aatgcagact cttcttaagt gttaataggg attttttcag 960cttattttgg ttgcagtttc caatttttaa aaatgttgag gtaatctttc ccaccttccc1020aaacctaatt cttgtagatg cattagtgtt gaaccaatgc ttctcatgtc tcaatcttgt1080
atatcatctt ttcagatgta ttaacaaaca aaaccttaaa aagagtagat gaattgccaa1140acacaattcc taccaataat aaatcgatca actctatcta ttcaggaaag caggaagcat1200ttggaccaca gtgcatgaaa acttcaacat tctgttatta gataatgaat caaccaaatg1260aacaatccag agaaaagaaa attgcaataa taaaaggtaa attaacagaa agataatata1320agcaagatag taatagttga ccattctgaa aagcttataa catcactcat catccagcat1380cctttctgaa aacaaaggat ttttaaatca ctttatgcac atatacaaca taggaggttg1440gcaaaataat gcactatttc ttaacagcca tgtctcttgt agaacttcaa gttaatctac1500aaatgaccat tgtgtcttaa tttagattat gaataccaca ttagtcaggt atttgcacta1560acccttaata gtatatacag tttctatgga aaattcagtg gtccaaaaat ttccgtagaa1620tttgagagga cgttggtggg ctgaagatag ctccttgagg gtcactgatg taggctgcaa1680tgggggttca caaggccctg acaccgtatt tatagtctaa cctttttatg aaaatctgac1740tacagctatt taaggagtag tcttaatagc tgaaaatgaa gatagagaaa gacaccaaga1800atatgacaca gtttacattc tagtgaggga cacaacaaaa tcaaatttaa aaaagagtgt1860aatagatgct gataaatact gtagataaag cacataagaa aatagaaata aaggctgtca1920atggagaagt catgattttt attttattta tttatttatt tatttgagac agagtcaggc1980tctgtgcagg ctggagtgca atggtgtgat ctcgctcact acaacctctg ctcctggctc2040aagctatcct cccacctcag ctctcaagta gctgggatca caggtgcgtg ctaccatgcc2100cggctaattt tttgtagaga tgaggttttg ccatgttgcc caggctggtc tcgaactcct2160ggactcaact gaccccacct cggcctctca aagtgctgag attataggcg tgcagccggc2220agctggccat tgtttatgtt ctgc 2244<210>20<211>351<212>DNA<213>人<220>
<221>misc_特征<222>(62)..(62)<223>任意堿基<220>
<221>misc_特征<222>(121)..(121)<223>任意堿基<220>
<221>misc_特征<222>(207)..(207)<223>任意堿基<220>
<221>misc_特征<222>(220)..(220)<223>任意堿基
<221>misc_特征<222>(276)..(276)<223>任意堿基<220>
<221>misc_特征<222>(300)..(300)<223>任意堿基<220>
<221>misc_特征<222>(315)..(315)<223>任意堿基<220>
<221>misc_特征<222>(336)..(336)<223>任意堿基<400>20tctacttcca catcggcgag accgagaagc gctgtttcat cgaggaaatc cccgacgaga 60cnatggtcat cggcaactat cgtacccaga tgtgggataa gcagaaggag gtcttcctgc 120nctcgacccc tggcctgggc atgcacgtgg aagtgaagga ccccgacggc aaggtggtgc 180tgtcctggca gtacggctcg gagggcnctt tcacgttcan ctcccacacg cccggtgacc 240atcaaatctg tctgcactcc aattcttacc aggatngctc tctttcgctg gtgggcaaan 300tgcgtgttgc atctngacat ccaggtttgg gggagnatgc caacaaatta c 351<210>21<211>2631<212>DNA<213>人<400>21accttccaac ccagccctcg gctgagccgc gccgcaccat gcccgccgtg gacaagctcc 60tgctagagga ggcgttgcag gacagccccc agactcgctc tttactgagc gtgtttgaag 120aagatgctgg caccctcaca gactatacca accagctgct ccaggcaatg cagcgcgtct 180atggagccca gaatgagatg tgcctggcca cacaacagct ttctaagcaa ctgctggcat 240atgaaaaaca gaactttgct cttggcaaag gtgatgaaga agtaatttca acactccact 300atttttccaa agtggtggat gagcttaatc ttctccatac agagctggct aaacagttgg 360cagacacaat ggttctacct atcatacaat tccgagaaaa ggatctcaca gaagtaagca 420ctttaaagga tctatttgga ctcgctagca atgagcatga cctctcaatg gcaaaataca 480gcaggctgcc taagaaaaag gagaatgaga aggtgaagac cgaagtcgga aaagaggtgg 540ccgcggcccg gcggaagcag cacctctcct cccttcagta ctactgtgcc ctcaacgcgc 600tgcagtacag aaagcaaatg gccatgatgg agcccatgat aggctttgcc catggacaga 660ttaacttttt taagaaggga gcagagatgt tttccaaacg tatggacagc tttttatcct 720
ccgttgcaga catggttcaa agcattcagg tagaactgga accgaggcgg aaaagatgcg 780ggtgtcccag caagaattac tttctgttga tgaatctgtt tacactccag actctgatgt 840ggccgcacca cagatcaaca ggaacctcat ccagaaggct ggttacctta atcttagaaa 900caaaacaggg ctggtcaccg ccacctggga gaggctttat ttcttcaccc aaggcgggaa 960tctcatgtgt cagcccaggg gagccgtggc tggaggtttg atccaggacc tggacaactg1020ctcagtgatg gccgtggatt gcgaagaccg gcgctactgc tttcagatca ccacgcccaa1080tggaaaatcg ggaataatcc tccaggctga gagcagaaag gaaaatgaag agtggatatg1140tgcaataaac aacatctcca gacagatcta cctgaccgac aaccctgagg cagtcgcgat1200caagttgaat cagaccgctc tgcaagcagt gactcctatt acaagttttg gaaaaaaaca1260agaaagctca tgccccagcc agaacctgaa aaattcagag atggaaaatg aaaatgacaa1320gattgttccc aaagcaacag ccagtctacc tgaagcagag gagctgatcg cgcctggagc1380gccgattcaa ttcgatattg tgcttcctgc tacagaattc cttgatcaga acagagggag1440caggcgtacc aacccttttg gtgaaactga ggatgaatca tttccagaag cagaagattc1500tcttttgcag cagatgttta tagttcggtt tttgggatca atggcagtta aaacagacag1560cactactgaa gtgatttatg aagcgatgag acaagtattg gctgctcggg ctattcataa1620catcttccgc atgacagaat cccatctgat ggtcaccagc caatctttga ggttgataga1680tccacagact caagtatcaa gggccaattt tgaacttacc agtgtcacac aatttgctgc1740tcatcaagaa aacaagagac tggttggttt tgtcatccgt gttcctgaat ccactggaga1800agaatctctg agtacataca tttttgaaag caactcagaa ggcgaaaaga tatgttatgc1860tattaatttg ggaaaagaaa ttattgaggt tcagaaggat ccagaagcac tggctcaatt1920aatgctgtcc ataccactaa ccaatgatgg aaaatatgta ctgttaaacg atcaaccaga1980tgacgatgat ggaaatccaa atgaacatag aggcgcagaa tccgaagcat aactcacttg2040cgcctgtggg ggaagagcga acaggaagga gagctacctc ctaagggttt taacgtctct2100gacatacagg cacactgacc tgatttccga aggctgacaa tcgtttgtgg aatgtaatct2160tgatgccttg atactgagac ttgggaggga aactaagaaa tggttgacag cgttcccacc2220catctacaat gttattttag gtgctttgtg gtaagtcttt tttcttagat tgcgctaaaa2280tttcttagat tgttcagcgc tcagaacaaa agtttgaaaa atgcattgtt catatgaatg2340tcatctcttt tcagtttcca gtatcctttt taaaaaatgg caaaagccta gatttacaat2400ttgatgaaca ctaaatattt cttattaata taatctattt ttgtatttta cttaatgagc2460tttaagtgcc tgtcgttctg aaaattgtgt atttataatt cagcttatct cataattgga2520cctaatagca tttctttgtg cagttaggtg atgagcactg ctttgaggcc caagcactag2580tagagatgcg cgatacaggt ctagtttcgg taactgttcc agacatcaag c 2631<210>22<211>2851<212>DNA<213>人
<400>22agcatctcag gccatcatcc tgaaacttgg cagccttcgt ggagtataag gacagcatta 60ttagccatca ttgggtttac tgccaacaaa aggagaggga gccataggtt ctctagatta 120cactcctgag gaaagaagag cacttgccaa aaaatcacaa gatttctgtt gtgaaggatg 180tggctctgcc atgaaggatg tcctgttgcc tttaaaatct ggaagcgatt caagccaagc 240tgaccaagaa gccaaagaac tggctaggca aataagcttt aaggcagaag tcaattcatc 300tggaaagact atctctgagt cagacttaaa ccactctttt tcactaactg atttacaaga 360tgatatacct acaacattcc agggtgctac ggccagtaca tcgtacggac tccagaattc 420ctcagcagca tcctttcatc aacctaccca acctgtagct aagaatacct ccatgagccc 480tcgacagcgc cgggcccagc agcagagtca gagaaggttg tctacttcac cagatgtaat 540ccagggccac cagccaagag acaaccacac tgatcatggt gggtcagctg tactgattgt 600catcctgact ttggcattgg cagctcttat attccgacga atatatctgg caaacgaata 660catatttgac tttgagttat aatatggttt tgtgacttat gagctgtgac tcaactgctt 720cattaaacat tctgcattgg gtataatcta agaattgttt acaaaaagat tattttgtat 780ttacccttca ttcctttttt tgatccttgt aagtttagta taaatatatc tagacattca 840gactgtgtct agcagttacg tcctgcttaa agggactaga agtcaaagtt ccttgtctca 900ctatttgatc tgctttgcag ggaaataact tgttttttct catgtttcat cttcttttta 960tgtaaatttg taatactttc ctatattgcc ctttgaaatt tttggataaa agatgatgtt1020ttaagttcca atgagtatta ctagttactc aataccactt attgagtact ctgtttctac1080gtatgtagaa tgtataggga tagaagagtt gaaaagggaa agcaaaactt cttaagtggc1140ttccttaaaa tgtcattcat aggagatgta ctggaattgc tcattctgtg actttatttg1200tgtcctaaac attcttcagt gaaaataatt ttatttcagt caaacattta tgaggaaatg1260agatcacatc tttgtcactg gatgctactt gaagagggag tactttgtaa ccactttgat1320atgctgttat caccaccccc tgccctctgc tgccataatc acacaaattt aaaaagaaag1380aaaacagtct tccatagatt tttaaggaag aaagggccca agtcaggaga tcgcttggtt1440ttcttccaga agttaaatgg ggggatctga agatttgaat gttcggtctg ctttgaaatg1500tatgtctttt gggaatgtat tatatgccta gctttataat caggtataaa attttaatta1560ttcccaggaa tatgcataat attgaatatt tcatgtccta ttttaataga aaacctcagg1620gcccaagtaa ccagtgatag aagttagaaa aaccccttta cttagaattg tccacctagt1680cagagcccaa gaaagaattt tcagtggaaa aatcaatata taacttagtg ctagctagcg1740ccacagactc tagtagataa tattatcatc ataatggctg gtgaaaccat ataatcacag1800aaaaacattg ccttcagcat gttcagttcg cagcactgag ggcactcttg agggtgttgt1860taatgaagat ttaattttta aatacaggtg gttccaagct ttcaaatagg ttatgctcca1920aaagtgttat ttgtaagtta atttttttac aagtcaaaca atgttggaag tggtatttag1980gttctagatc ggtccacgaa agttagccca tatgtatatc ttgaatagta taggggaggg2040
tattcataaa gtccttatgt ggttttaact aagtgaaatt atggacaaga gaaataattg2100taaaatcgtc ttaaaggcaa atttaatttt tacccctgtt tatgggacat tcgttctatt2160aactgtcaga cacaatttct gttttcatct gagagccagg tttcctttat ttctacatct2220aaaataagaa catattgtac actattatat aatacagaat tgtcttacac tttaataaat2280tcgcatttta aaggtgttta caggattatt ttttatatct gtagctgaat ttgttaaagt2340ctaaaaagct caaggacttt atgaagatct cattatatga ggaaaatcat aggttaccat2400tttataactc tattgccata agaaaataca ctctaaaatc ttgatttgaa acatattaga2460aaccttgatt cagtgctcag tggtctccta gtaagaagtc accgacggta gcgtcatatg2520agaagaaaga aatccccacc acctcaacct ctgctgagat tgtgtgctag gaacagcctt2580ccctccgttt cccctcagtc aaacttgagc cagcctctgg atcgatgtga tcttattgca2640tgtttccatg gggtgtacct atactttaag ccaatcctgc tgcattcact gctaagttaa2700ataaaaagcc aagaagaaaa aaaaaatttt gcactgtgca gatcctttgc tatctgactt2760gcatctcttc ccccacctgt cagctagcca cctgcttgtt tgtgttggga tattttttag2820cacctgaagc accatctgaa aggggcacca t 2851<210>23<211>3473<212>DNA<213>人<400>23aagagcagcg gcgaggcggc ggtggtggct gagtccgtgg tggcagaggc gaaggcgaca 60gctctagggg ttggcaccgg ccccgagagg aggatgcggg tccggatagg gctgacgctg 120ctgctgtgtg cggtgctgct gagcttggcc tcggcgtcct cggatgaaga aggcagccag 180gatgaatcct tagattccaa gactactttg acatcagatg agtcagtaaa ggaccacact 240actgcaggca gagtagttgc tggtcaaata tttcttgatt cagaagaatc tgaattagaa 300tcctctattc aagaagagga agacagcctc aagagccaag agggggagag tgtcacagaa 360gatatcagct ttctagagtc tccaaatcca gaaaacaagg actatgaaga gccaaagaaa 420gtacggaaac cagctttgac cgccattgaa ggcacagcac atggggagcc ctgccacttc 480ccttttcttt tcctagataa ggagtatgat gaatgtacat cagatgggag ggaagatggc 540agactgtggt gtgctacaac ctatgactac aaagcagatg aaaagtgggg cttttgtgaa 600actgaagaag aggctgctaa gagacggcag atgcaggaag cagaaatggt gtatcaaact 660ggaatgaaaa tccttaatgg aagcaataag aaaagccaaa aaagagaagc atatcggtat 720ctccaaaagg cagcaagcat gaaccatacc aaagccctgg agagagtgtc atatgctctt 780ttatttggtg attacttgcc acagaatatc caggcagcga gagagatgtt tgagaagctg 840actgaggaag gctctcccaa gggacagact gctcttggct ttctgtatgc ctctggactt 900ggtgttaatt caagtcaggc aaaggctctt gtatattata catttggagc tcttgggggc 960aatctaatag cccacatggt tttgggttac agatactggg ctggcatcgg cgtcctccag1020
agttgtgaat ctgccctgac tcactatcgt cttgttgcca atcatgttgc tagtgatatc1080tcgctaacag gaggctcagt agtacagaga atacggctgc ctgatgaagt ggaaaatcca1140ggaatgaaca gtggaatgct agaagaagat ttgattcaat attaccagtt cctagctgaa1200aaaggtgatg tacaagcaca ggttggtctt ggacaactgc acctgcacgg agggcgtgga1260gtagaacaga atcatcagag agcatttgac tacttcaatt tagcagcaaa tgctggcaat1320tcacatgcca tggccttttt gggaaagatg tattcggaag gaagtgacat tgtacctcag1380agtaatgaga cagctctcca ctactttaag aaagctgctg acatgggcaa cccagttgga1440cagagtgggc ttggaatggc ctacctctat gggagaggag ttcaagttaa ttatgatcta1500gcccttaagt atttccagaa agctgctgaa caaggctggg tggatgggca gctacagctt1560ggttccatgt actataatgg cattggagtc aagagagatt ataaacaggc cttgaagtat1620tttaatttag cttctcaggg aggccatatc ttggctttct ataacctagc tcagatgcat1680gccagtggca ccggcgtgat gcgatcatgt cacactgcag tggagttgtt taagaatgta1740tgtgaacgag gccgttggtc tgaaaggctt atgactgcct ataacagcta taaagatggc1800gattacaatg ctgcagtgat ccagtacctc ctcctggctg aacagggcta tgaagtggca1860caaagcaatg cagcctttat tcttgatcag agagaagcaa gcattgtagg tgagaatgaa1920acttatccca gagctttgct acattggaac agggccgcct ctcaaggcta tactgtggct1980agaattaagc tcggagacta ccatttctat gggtttggca ccgatgtaga ttatgaaact2040gcatttattc attaccgtct ggcttctgag cagcaacaca gtgcacaagc tatgtttaat2100ctgggatata tgcatgagaa aggactgggc attaaacagg atattcacct tgcgaaacgt2160ttttatgaca tggcagctga agccagccca gatgcacaag ttccagtctt cctagccctc2220tgcaaattgg gcgtcgtcta tttcttgcag tacatacggg aaacaaacat tcgagatatg2280ttcacccaac ttgatatgga ccagcttttg ggacctgagt gggaccttta cctcatgacc2340atcattgcgc tgctgttggg aacagtcata gcttacaggc aaaggcagca ccaagacatg2400cctgcaccca ggcctccagg gccacggcca gctccacccc agcaggaggg gccaccagag2460cagcagccac cacagtaata ggcactgggt ccagccttga tcagtgacag cgaaggaagt2520tatctgctgg gaacacttgc atttgattta ggaccttgga tcagtggtca cctcccagaa2580gaggcacggc acaaggaagc attgaattcc taaagctgct tagaatctga tgcctttatt2640ttcagggata agtaactctt acctaaactg agctgaatgt ttgtttcagt gccatatgga2700ataacaactt tcagtggctt ttttttttct tttctggaaa catatgtgag acactcagag2760taatgtctac tgtatccagc tatctttctt ggatcctttt ggtcattatt tcagtgtgca2820taagttctta atgtcaacca tctttaaggt attgtgcatc gacactaaaa actgatcagt2880gtaaaaagga aaacccagtt gcaagtttaa acgtgttcga aagtctgaaa atagaacttg2940ccttttaagt taaaaaaaaa aaaagctatc ttgaaaatgt tttggaactg cgataactga3000gaaactctta ccagtccaca tgcaattaga catattcagc atatttgtta ttttaaaagg3060
gagggttggg aggtttctta ttggtgattg tcacacggta taccatactc ctctccttca3120aagaatgaaa ggccttgtta aggagttttt tgtgagcttt acttctttgg aatggaatat3180acttatgcaa aaccttgtga actgactcct tgcactaacg cgagtttgcc ccacctactc3240tgtaatttgc ttgtttgttt tgaatataca gagccttgat ccagaagcca gaggatggac3300taagtgggag aaattagaaa acaaaacgaa ctctggttgg ggtactacga tcacagacac3360agacatactt ttcctaaagt tgaagcattt gttcccagga tttattttac tttgcatttc3420cttttgcaca aagaacacat caccatttcc ttttgcacaa agaacacatc acc 3473<210>24<211>401<212>DNA<213>人<220>
<221>misc_特征<222>(252)..(252)<223>任意堿基<220>
<221>misc_特征<222>(303)..(303)<223>任意堿基<220>
<220>misc_特征<222>(390)..(390)<223>任意堿基<400>24ttagattatt ttcaatttat tattcagaat aaatatatct tttttcttta acttctcaaa 60tagttattga attgtattgg tttaaattaa atgcgtcatg tgtatatatc agtattaatt 120caagagatac aaaaggaaat tgagtgaaaa ataagtctgc ctccttccca tcactctcat 180gtctctacct agaggcaatt attgtcaaca gtttttgatg tgtctttcaa aaaatagtcc 240attaagcctg gngtactaga tctcttttaa aagtttacaa cctgttacag aatatatata 300aangttcaat tactagtaac accttattac agatacagat tacaacttag gaaatatatt 360ttcatggacc attgatgtca tttggattcn cccctacaat c 401<210>25<211>1820<212>DNA<213>人<400>25aatgtcttag aaaaaggctt tctaaaagaa aaagagcaag aggccatttc ttttcaagat 60agatacaaag aacttcagga aaaacataaa caagaattgg aagacatgag gaaagctggt 120cacgaagccc tcagcattat tgtggatgaa tataaggcac tactgcagtc ttcagttaag 180caacaagtag aagctattga aaaacagtac atttctgcaa ttgagaaaca ggcacacaag 240tgtgaggagt tgctaaatgc tcagcatcag aggctccttg aaatgctaga tacagagaag 300
gaactgttaa aagaaaaaat aaaggaagct ttgattcagc aatctcaaga acagaaggaa 360atattggaaa agtgtttgga ggaagaaagg caaagaaata aagaggcatt agtatccgct 420gcaaagcttg aaaaagaagc agtgaaggat gcagttttaa aagtcgtaga agaagaaaga 480aaaaatttag aaaaagcgca tgctgaagaa agggaattat ggaagacaga acatgcaaaa 540gatcaagaaa aagtatctca ggaaattcaa aaagctatac aagaacaaag aaaaataagt 600caggaaactg ttaaggcagc aataatagaa gagcagaaac gaagtgaaaa ggctgtggaa 660gaggcagtga aaagaacaag agatgaattg atagagtata taaaagaaca gaaaaggctc 720gatcaagtca tccgccaaag aagcctgtcc agtttggaac tgttcctctc ctgtgcacag 780aaacagttaa gtgctttaat agctacggaa ccagttgaca ttgaataaaa agaacatgac 840aaacccacac tggcattgga taaatcatat tacaccttca aaatacacac tctgaattat 900aaagatgtgt ttgttttctt tccaaatcat gtagaattga tttccagttc aaggataaac 960caaaacaata tttagaacta tcaagtgatc taatttattt tcttttggtt tcttctttac1020atttactgtt attttattat tattagtagt agcagcaaca gagtatgata tgacccaaaa1080gccattgtaa agtgccacat taccaaaatt aattaagtaa actttatagc ctgtgggagt1140ctattatata ttattttgca aaagtagtaa atatattatt gtttcatgat gactcttgat1200gagatgctag aatgtaacca tacatttatc ttattttgag gatagaaata gcatggattt1260caacatcact tatttatctg tataattgga aataaaacac cgatatgata gagaatcatt1320ccggcattac ctaacctctt ctgcagttgg atctatgtat tttcattggt ctactgaaaa1380cgaacaatac aattaaaagc actaaagatt attatattaa ttcaactttg atctgatata1440tcacttaaac taaaggggtg tgtgtggtgt atgcttgttt cctatttctg ctctttaaag1500atactttgaa tcaataaaac cattagtcta caaatcaaat tgtgaactta atctctagaa1560agagaatata actcagccat ttataggaat ttaggttcaa gtacaggata tatgaaatct1620tttcccagta tttcagaatg tacttaattc acaggcagga tgcttcaatg caaaatcatg1680aatattttta attcaaaact aaaatgtcat taatatgtat gtatgcaaat gttttatctt1740attttctgaa atgcatctac tttcatgggc tttgtacgtt tctgagattt ctcagtgtaa1800taaaaagagc tcccaaactt1820<210>26<211>280<212>DNA<213>人<220>
<221>misc_特征<222>(261)..(261)<223>任意堿基<220>
<221>misc_特征<222>(237)..(237)<223>任意堿基
<400>26tcaagtcata agataaagtt taatcatttg atcatgttaa aagacacaaa acacagccaa 60tctaaccaaa tttcaggcat gcatttacat aaatatatta aattaagaaa agaaattgta 120cacttaaacg tccttttcac ctagaaatca ttaaatccac agatcaacaa taaaaccaat 180tctctgcatt taccacttca agatacaatt gttctatttt aaagataaca caaactncac 240tagtctggtt aggaatttat ntgcattata catatattat 280<210>27<211>392<212>DNA<213>人<400>27ttggtttgaa atggcacccc aggactttgg gcctgcctta cttgatagcc tcgttcagtg 60agcaaagact tagtgagcag ctcttgtatg ccaagtattt tgctaagctc tggaaaaaag 120ataaacaaga catggttctt gctttcaagg agtgtgtaat tctttagcca gatatggaaa 180cctggaccct gagtgggaga aaggagacag atgaaaggag tccgtgattt tgtaaccaag 240agctgcctgc atggttatga gtatcactga ttttagggac gcccacagag ctaaagcatt 300tttttaatcc gagaagactt ttgtaactca tattagttaa tcttctagct ctgagatagc 360aacacagctc ttagaattct gtaagtaagc tt 392<210>28<211>2299<212>DNA<213>人<400>28cgaaccccca cagctggagg gcgaggccag ctgtacccgg ccccagtgcc ctttcgcggc 60cacaagcggc cgtcctcctg gtccggtgct ccggcgcctg atctaggttc atggagccgg 120ggctgtggct ccttttcggg ctcacagtga cctccgccgc aggattcgtg ccttgctccc 180agtctgggga tgctggcagg cgcggcgtgt cccaggcccc cactgcagcc agatctgagg 240gggactgtga agagactgtg gctggccctg gcgaggagac tgtggctggc cctggcgagg 300ggactgtggc cccgacagca ctgcagggtc caagccctgg aagccctggg caggagcagg 360cggccgaggg ggcccctgag caccaccgat ccaggcgctg cacgtgcttc acctacaagg 420acaaggagtg tgtctactat tgccacctgg acatcatttg gatcaacact cccgaacaga 480cggtgcccta tggactgtcc aactacagag gaagcttccg gggcaagagg tctgcggggc 540cacttccagg gaatctgcag ctctcacatc ggccacactt gcgctgcgct tgtgtgggga 600gatatgacaa ggcctgcctg cacttttgca cccaaactct ggacgtcagc agacaggttg 660aagtcaagga ccaacaaagc aagcaggctt tagacctcca ccatccaaag ctcatgcccg 720gcagtggact cgccctcgct ccatctacct gcccccgctg cctctttcag gaaggagccc 780cttaggagga caggcctgca gcatcctggt ctcgggaggc ttctgtcatt gctcacacac 840
agttcagatt tccacctctt tatagacaag aagtgaattt gcctggggca gaacacccac 900ccaaagagtc cccacttaac aatacccccc ccccacggca agaatgccca aatccgaatg 960accccagttt tcctaatgag taaaatgatc ccagatgtgc cccagagcat gacgcctgca1020gctccggttt catgcaggaa attggttttg gagagttttg gcaagttgga aagccactta1080ctggcttttg acatgacttc tcttggagaa taagtggact ccaagctaac tctttgcaaa1140tgtaaacaca tgtccatctt gtaataaatg caaaatgccc gtgcagcaga agcatgcgac1200tttcatatcc ttgcctagaa taggctgcat ggtgtatgtc agtgagggcc acgaggcgtc1260ggctttagac acagatcata gctctacagg agtttatgaa tttgaagctt atgggatttt1320ggcagagaaa ttttcagctg tgcttgatac ccaccaaaag aatgtatctc gaaagaatga1380aggaagaaga aaaaaggatc cttgatgttt gtgacaagaa aatgagaaag ttagtatctg1440caatacagag cttgttcctg ttcagtgact gaccctctgt attctgtata gacaccaggc1500cgatacacag tggagttccc aggccttgtt tgcaggaagc cgactgtaaa gacagcccca1560gctcaaggct attaggttga atatttgctt tcatgagtaa atgtggatct ttggggaatg1620gcttcaaaat aagtcacgaa cacaaattct ttgtaaatta tgtaaattcc tgtttatata1680aattggcaac aacttatacc gtctgacagt tcaaaatctc tttcagctgc gctcttccca1740ccgagccgag cttactgtga gtgtggagat gttatcccac catgtaaagt cgcctgcgca1800ggggagggct gcccatctcc ccaacccagt cacagagaga taggaaacgg catttgagtg1860ggtgtccagg gccccgtaga gagacattta agatggtgta tgacagagca ttggccttga1920ccaaatgtta aatcctctgt gtgtatttca taagttatta caggtataaa agtgatgacc1980tatcatgagg aaatgaaagt ggctgatttg ctggtaggat tttgtacagt ttagagaagc2040gattatttat tgtgaaactg ttctccactc caactccttt atgtggatct gttcaaagta2100gtcactgtat atacgtatag agaggtagat aggtaggtag attttaaatt gcattctgaa2160tacaaactca tactccttag agcttgaatt acatttttaa aatgcatatg tgctgtttgg2220caccgtggca agatggtatc agagagaaac ccatcaattg ctcaaatact cagaaagtac2280tgtcaaaagc ctaataaaa 2299<210>29<211>1339<212>DNA<213>人<400>29ctaaacaaaa tcattcactt ccctgatttt gataagaaaa ttcctgtaaa gctgtttcct 60ctgcctctcc tctacgttgg aaaccacata agtggattat caagcacaag taaattaagc 120ctaccgatgt tcaccgtgct caggaaattc accattccac ttaccttact tctggaaacc 180atcatacttg ggaagcagta ttcactcaac atcatcctca gtgtctttgc cattattctc 240ggggctttca tagcagctgg gtctgacctt gcttttaact tagaaggcta tatttttgta 300ttcctgaatg atatcttcac atcagcaaat ggagtttata ccaaacagaa aatggaccca 360
aaggagctag ggaaatacgg agtacttttc tacaatgcct gcttcatgat tatcccaact 420cttattatta gtgtctccac tggagacctc caacaggcta ctgaattcaa ccaatggaag 480aatgttgtgt ttatcctaca gtttcttctt tcctgttttt tggggtttct gctgatgtac 540tccacggttc tgtgcagcta ttacaattca gccctgacga cagcagtggt tggagccatc 600aagaatgtat ccgttgccta cattgggata ttaatcggtg gagactacat tttctctttg 660ttaaactttg tagggttaaa tatttgcatg gcagggggct tgagatattc ctttttaaca 720ctgagcagcc agttaaaacc taaacctgtg ggtgaagaaa acatctgttt ggatttgaag 780agctaaagag tctgcagcag gattggagac tgacttgtga ctgcgggctg ggggggcatt 840cccagtagga atgtgaagcc agaggtttcg gattcgtgac atccaccccc tgggcaagtg 900agagcatctg caaaatgcaa agagaactac ctcatatgca ggatgagcca atggcagtct 960caagaaatgt actcgggcga caccttacct gtggaaagca aatcttttca aaataagcca1020ctgggactcg gtaggtggag ccccagctgc tcttctaggg acctatgggg ccttcgtggc1080atctctgtgc tgtgtgctgg ggaggaggtt gatgtaatgg tgactctttt ctgatcagca1140ccttggccgt gattcccaag gtcccagcca aagcaaaggg ccagttgttt cagtttaaac1200agacatgtct ttagtctaat aaaattagtt aactgccagt aaagttattt gttagctttg1260atgaaagcta tgttggtatc tttccctaat catcaaagta aataaaaaat catttctatg1320taaaaaaaaa aaaaaaaaa 1339<210>30<211>4250<212>DNA<213>人<400>30gaacacatcg cgtttgcatc ccagaaagta gtcgccgcga ctatttcccc caaagagaca 60agcacacatg taggaatgac aaaggcttgc gaaggagaga gcgcagcccg cggcccggag 120agatcccctc gataatggat tactaaatgg gatacacgct gtaccagttc gctccgagcc 180ccggccgcct gtccgtcgat gcaccgaaaa gggtgaagta gagaaataaa gtctccccgc 240tgaactacta tgaggtcaga agccttgctg ctatatttca cactgctaca ctttgctggg 300gctggtttcc cagaagattc tgagccaatc agtatttcgc atggcaacta tacaaaacag 360tatccggtgt ttgtgggcca caagccagga cggaacacca cacagaggca caggctggac 420atccagatga ttatgatcat gaacggaacc ctctacattg ctgctaggga ccatatttat 480actgttgata tagacacatc acacacggaa gaaatttatt gtagcaaaaa actgacatgg 540aaatctagac aggccgatgt agacacatgc agaatgaagg gaaaacataa ggatgagtgc 600cacaacttta ttaaagttct tctaaagaaa aacgatgatg cattgtttgt ctgtggaact 660aatgccttca acccttcctg cagaaactat aagatggata cattggaacc attcggggat 720gaattcagcg gaatggccag atgcccatat gatgccaaac atgccaacgt tgcactgttt 780gcagatggaa aactatactc agccacagtg actgacttcc ttgccattga cgcagtcatt 840
taccggagtc ttggagaaag ccctaccctg cggaccgtca agcacgattc aaaatggttg 900aaagaaccat actttgttca agccgtggat tacggagatt atatctactt cttcttcagg 960gaaatagcag tggagtataa caccatggga aaggtagttt tcccaagagt ggctcaggtt1020tgtaagaatg atatgggagg atctcaaaga gtcctggaga aacagtggac gtcgttcctg1080aaggcgcgct tgaactgctc agttcctgga gactctcatt tttatttcaa cattctccag1140gcagttacag atgtgattcg tatcaacggg cgtgatgttg tcctggcaac gttttctaca1200ccttataaca gcatccctgg gtctgcagtc tgtgcctatg acatgcttga cattgccagt1260gtttttactg ggagattcaa ggaacagaag tctcctgatt ccacctggac accagttcct1320gatgaacgag ttcctaagcc caggccaggt tgctgtgctg gctcatcctc cttagaaaga1380tatgcaacct ccaatgagtt ccctgatgat accctgaact tcatcaagac gcacccgctc1440atggatgagg cagtgccctc catcttcaac aggccatggt tcctgagaac aatggtcaga1500taccgcctta ccaaaattgc agtggacaca gctgctgggc catatcagaa tcacactgtg1560gtttttctgg gatcagagaa gggaatcatc ttgaagtttt tggccagaat aggaaatagt1620ggttttctaa atgacagcct tttcctggag gagatgagtg tttacaactc tgaaaaatgc1680agctatgatg gagtcgaaga caaaaggatc atgggcatgc agctggacag agcaagcagc1740tctctgtatg ttgcgttctc tacctgtgtg ataaaggttc cccttggccg gtgtgaacga1800catgggaagt gtaaaaaaac ctgtattgcc tccagagacc catattgtgg atggataaag1860gaaggtggtg cctgcagcca tttatcaccc aacagcagac tgacttttga gcaggacata1920gagcgtggca atacagatgg tctgggggac tgtcacaatt cctttgtggc actgaatgac1980atttcaactc ctctaccaga taatgaaatg tcttacaaca cagtgtatgg gcattccagt2040tccctcttgc ccagcacaac cacatcagat tcgacggctc aagaggggta tgagtctagg2100ggaggaatgc tggactggaa gcatctgctt gactcacctg acagcacaga ccctttgggg2160gcagtgtctt cccataatca ccaagacaag aagggagtga ttcgggaaag ttacctcaaa2220ggccacgacc agctggttcc cgtcaccctc ttggccattg cagtcatcct ggctttcgtc2280atgggggccg tcttctcggg catcaccgtc tactgcgtct gtgatcatcg gcgcaaagac2340gtggctgtgg tgcagcgcaa ggagaaggag ctcacccact cgcgccgggg ctccatgagc2400agcgtcacca agctcagcgg cctctttggg gacactcaat ccaaagaccc aaagccggag2460gccatcctca cgccactcat gcacaacggc aagctcgcca ctcccggcaa cacggccaag2520atgctcatta aagcagacca gcaccacctg gacctgacgg ccctccccac cccagagtca2580accccaacgc tgcagcagaa gcggaagccc agccgcggca gccgcgagtg ggagaggaac2640cagaacctca tcaatgcctg cacaaaggac atgcccccca tgggctcccc tgtgattccc2700acggacctgc ccctgcgggc ctcccccagc cacatcccca gcgtggtggt cctgcccatc2760acgcagcagg gctaccagca tgagtacgtg gaccagccca aaatgagcga ggtggcccag2820atggcgctgg aggaccaggc cgccacactg gagtataaga ccatcaagga acatctcagc2880agcaagagtc ccaaccatgg ggtgaacctt gtggagaacc tggacagcct gccccccaaa2940
gttccacagc gggaggcctc cctgggtccc ccgggagcct ccctgtctca gaccggtcta3000agcaagcggc tggaaatgca ccactcctct tcctacgggg ttgactataa gaggagctac3060cccacgaact cgctcacgag aagccaccag gccaccactc tcaaaagaaa caacactaac3120tcctccaatt cctctcacct ctccagaaac cagagctttg gcaggggaga caacccgccg3180cccgccccgc agagggtgga ctccatccag gtgcacagct cccagccatc tggccaggcc3240gtgactgtct cgaggcagcc cagcctcaac gcctacaact cactgacaag gtcggggctg3300aagcgtacgc cctcgctaaa gccggacgta ccccccaaac catcctttgc tcccctttcc3360acatccatga agcccaatga tgcgtgtaca taatcccagg gggagggggt caggtgtcga3420accagcaggc aaggcgaggt gcccgctcag ctcagcaagg ttctcaactg cctcgagtac3480ccaccagacc aagaaggcct gcggcagagc cgaggacgct gggtcctcct ctctgggaca3540caggggtact cacgaaaact gggccgcgtg gtttggtgaa ggtttgcaac ggcggggact3600caccttcatt ctcttccttc actttccccc acaccctaca acaggtcgga cccacaaaag3660acttcagtta tcatcacaaa catgagccaa aagcacatac ctaccccatc ccccaccccc3720acacacacac acacatgcac acaacacata cacacacacg cacagaggtg aacagaaact3780gaaacatttt gtccacaact tcacgggacg tggccagact gggtttgcgt tccaacctgc3840aaaacacaaa tacatttttt aaaatcaaga aaatttaaaa agacaaaaaa aaaagaattc3900attgataatt ctaactcaga ctttaacaat ggcagaagtt tactatgcgc aaatactgtg3960aaatgcccgc cagtgttaca gctttctgtt gcagcagata aatgccatgt tgggcaacta4020tgtcatagat ttctgctcct cctctctttt aatgaaataa cgtgaccgtt aacgcaagta4080actctttatt tattgttcac cctttttttc cttaaggaaa ggactcttcc aaatatcatc4140ctatgaacag ctcttcagaa agcccattga aagttaaact atttaacgtg aaatccatta4200actggaataa ttgagtttct ttatttttac aataaattca ctgagtaaat 4250<210>31<211>2785<212>DNA<213>人<400>31ctttagccca acagtcaaaa ataattgatg ctaccctaca aatgtccaaa actctagtat 60atcatatttc taagttacag caaatattag tcctgctaaa ccagggagct ttggcaaaaa 120tgttttttga cagtaaattt gtccttgatt atatattaac tagtcaaaga ggtgtttgta 180acattattag agcttcttgt tgtaggtggg ttaacaccac caatcaagag gtcattctaa 240cagaaagcct ggatcagaaa accatcaccc taaaaaaaca tgccttacat atttaacaca 300ctctgaaatc cagtcaaaat atgactaaag gcccttgcca tgactgatgt attctcctgg 360ccaacgccaa acaaatggga gcctggttac gagtcagcct tcagggactt gtcacatttc 420tacttggttt cttccttgtt attgtcataa taaaatgttt tctatgctgt ttagtgcaac 480ttaggcccta ttctgtagaa gtctcctcta ctattcaggc cactcaaaca ccccaaataa 540
ttgagttcaa aatcgacatc aagatataaa ggaatcagtg actaaatata tttcatatat 600ggtattttta ttgattattg tgctgtcttg acctagtatg gaggccttgg ctagaggctg 660gtcagtttcc tctcttgagc agctgattaa atccacaccc caaccacttc ccttatcagg 720ttctcacact ctggggccac tatgtaccca ctctaatcac cacagggcca gacatcagac 780aattaaggac agcgcccatg ccccaaagcc cgccaaaatt atgcaaatta ttcaaaatta 840ttcaacctag ctaaccccac cctttttgct gtacataagc tgcccattcc ccctccagcc 900tgtggtaccc agtcctcagg tgcaaccccc tgcgtggtcc tctgtggcag ccttctctca 960ttcagagctg ttttccacag aggtagtgaa aagaactgga ttttcaagtt cactttgcaa1020gagaaaaaga aaactcagta gaagataatg gcaagtccag actggggata tgatgacaaa1080aatggtcctg aacaatggag caagctgtat cccattgcca atggaaataa ccaatcccct1140gttgatatta aaaccagtga aaccaaacat gacacctctc tgaaacctat tagtgtctcc1200tacaacccag ccacagccaa agaaattatc aatgtggggc attctttcca tgtaaatttt1260gaggacaacg ataaccgatc agtgctgaaa ggtggtcctt tctctgacag ctacaggctc1320tttcagtttc attttcactg gggcagtaca aatgagcatg gttcagaaca tacagtggat1380ggagtcaaat attctgccga gcttcacgta gctcactgga attctgcaaa gtactccagc1440cttgctgaag ctgcctcaaa ggctgatggt ttggcagtta ttggtgtttt gatgaaggtt1500ggtgaggcca acccaaagct gcagaaagta cttgatgccc tccaagcaat taaaaccaag1560ggcaaacgag ccccattcac aaattttgac ccctctactc tccttccttc atccctggat1620ttctggacct accctggctc tctgactcat cctcctcttt atgagagtgt aacttggatc1680atctgtaagg agagcatcag tgtcagctca gagcagctgg cacaattccg cagccttcta1740tcaaatgttg aaggtgataa cgctgtcccc atgcagcaca acaaccgccc aacccaacct1800ctgaagggca gaacagtgag agcttcattt tgatgattct gagaagaaac ttgtccttcc1860tcaagaacac agccctgctt ctgacataat ccagttaaaa taataatttt taagaaataa1920atttatttca atattagcaa gacagcatgc cttcaaatca atctgtaaaa ctaagaaact1980taaattttag ttcttactgc ttaattcaaa taataattag taagctagca aatagtaatc2040tgtaagcata agcttatctt aaattcaagt ttagtttgag gaattcttta aaattacaac2100taagtgattt gtatgtctat ttttttcagt ttatttgaac caataaaata attttatctc2160tttctttctg ttgtgcattc agtttctaaa accattaagt ttctactcca tttacattca2220aaaatcttaa atactttact tgcaagagta ttttgcttca aatacaacaa cctaagagca2280gctggagatg aaatattggg aaattcattt gcttactcct gaagacaaaa atatagctga2340gatgaccact ggatttaata tcgttatgct ggcccaacat tgctaccatt tgtgttgtct2400gtgatcaaaa tgattatctt ttatatagga agatgacgct tctggatatt gctttcactt2460cttctcccca cgttagcaag gacaatgctt ctctgccatt attacaacta gttagtttgc2520atggagaatc tttactttaa aattggaaga aaagtcacaa gtgaatggtt tataaaaatg2580
ctaaagaagt cattcttgct tagaatcata tagaaacatc atgcaatctt ttagtcagat2640gtgcgcttca ccttatgcta tttttatctt taattgacac acaataattg tacatgttta2700tggagtatag tgtggtgttt tctgtttgtt tgtttgtttt ttgagacaag gtctcactct2760gccagtcagg gtggagtgcg atggt 2785<210>32<211>9588<212>DNA<213>人<400>32ccgaccaaca ccaacaccca gctccgacgc agctcctctg cgcccttgcc gccctccgag 60ccacagcttt cctcccgctc ctgcccccgg cccgtcgccg tctccgcgct cgcagcggcc 120tcgggagggc ccaggtagcg agcagcgacc tcgcgagcct tccgcactcc cgcccggttc 180cccggccgtc cgcctatcct tggccccctc cgctttctcc gcgccggccc gcctcgctta 240tgcctcggcg ctgagccgct ctcccgattg cccgccgaca tgagctgcaa cggaggctcc 300cacccgcgga tcaacactct gggccgcatg atccgcgccg agtctggccc ggacctgcgc 360tacgaggtga ccagcggcgg cgggggcacc agcaggatgt actattctcg gcgcggcgtg 420atcaccgacc agaactcgga cggctactgt caaaccggca cgatgtccag gcaccagaac 480cagaacacca tccaggagct gctgcagaac tgctccgact gcttgatgcg agcagagctc 540atcgtgcagc ctgaattgaa gtatggagat ggaatacaac tgactcggag tcgagaattg 600gatgagtgtt ttgcccaggc caatgaccaa atggaaatcc tcgacagctt gatcagagag 660atgcggcaga tgggccagcc ctgtgatgct taccagaaaa ggcttcttca gctccaagag 720caaatgcgag ccctttataa agccatcagt gtccctcgag tccgcagggc cagctccaag 780ggtggtggag gctacacttg tcagagtggc tctggctggg atgagttcac caaacatgtc 840accagtgaat gtttggggtg gatgaggcag caaagggcgg agatggacat ggtggcctgg 900ggtgtggacc tggcctcagt ggagcagcac attaacagcc accggggcat ccacaactcc 960atcggcgact atcgctggca gctggacaaa atcaaagccg acctgcgcga gaaatctgcg1020atctaccagt tggaggagga gtatgaaaac ctgctgaaag cgtcctttga gaggatggat1080cacctgcgac agctgcagaa catcattcag gccacgtcca gggagatcat gtggatcaat1140gactgcgagg aggaggagct gctgtacgac tggagcgaca agaacaccaa catcgctcag1200aaacaggagg ccttctccat acgcatgagt caactggaag ttaaagaaaa agagctcaat1260aagctgaaac aagaaagtga ccaacttgtc ctcaatcagc atccagcttc agacaaaatt1320gaggcctata tggacactct gcagacgcag tggagttgga ttcttcagat caccaagtgc1380attgatgttc atctgaaaga aaatgctgcc tactttcagt tttttgaaga ggcgcagtct1440actgaagcat acctgaaggg gctccaggac tccatcagga agaagtaccc ctgcgacaag1500aacatgcccc tgcagcacct gctggaacag atcaaggagc tggagaaaga acgagagaaa1560atccttgaat acaagcgtca ggtgcagaac ttggtaaaca agtctaagaa gattgtacag1620
ctgaagcctc gtaacccaga ctacagaagc aataaaccca ttattctcag agctctctgt1680gactacaaac aagatcagaa aatcgtgcat aagggggatg agtgtatcct gaaggacaac1740aacgagcgca gcaagtggta cgtgacgggc ccgggaggcg ttgacatgct tgttccctct1800gtggggctga tcatccctcc tccgaaccca ctggccgtgg acctctcttg caagattgag1860cagtactacg aagccatctt ggctctgtgg aaccagctct acatcaacat gaagagcctg1920gtgtcctggc actactgcat gattgacata gagaagatca gggccatgac aatcgccaag1980ctgaaaacaa tgcggcagga agattacatg aagacgatag ccgaccttga gttacattac2040caagagttca tcagaaatag ccaaggctca gagatgtttg gagatgatga caagcggaaa2100atacagtctc agttcaccga tgcccagaag cattaccaga ccctggtcat tcagctccct2160ggctatcccc agcaccagac agtgaccaca actgaaatca ctcatcatgg aacctgccaa2220gatgtcaacc ataataaagt aattgaaacc aacagagaaa atgacaagca agaaacatgg2280atgctgatgg agctgcagaa gattcgcagg cagatagagc actgcgaggg caggatgact2340ctcaaaaacc tccctctagc agaccagggg tcttctcacc acatcacagt gaaaattaac2400gagcttaaga gtgtgcagaa tgattcacaa gcaattgctg aggttctcaa ccagcttaaa2460gatatgcttg ccaacttcag aggttctgaa aagtactgct atttacagaa tgaagtattt2520ggactatttc agaaactgga aaatatcaat ggtgttacag atggctactt aaatagctta2580tgcacagtaa gggcactgct ccaggctatt ctccaaacag aagacatgtt aaaggtttat2640gaagccaggc tcactgagga ggaaactgtc tgcctggacc tggataaagt ggaagcttac2700cgctgtggac tgaagaaaat aaaaaatgac ttgaacttga agaagtcgtt gttggccact2760atgaagacag aactacagaa agcccagcag atccactctc agacttcaca gcagtatcca2820ctttatgatc tggacttggg caagttcggt gaaaaagtca cacagctgac agaccgctgg2880caaaggatag ataaacagat cgactttaga ttatgggacc tggagaaaca aatcaagcaa2940ttgaggaatt atcgtgataa ctatcaggct ttctgcaagt ggctctatga tcgtaaacgc3000cgccaggatt ccttagaatc catgaaattt ggagattcca acacagtcat gcggtttttg3060aatgagcaga agaacttgca cagtgaaata tctggcaaac gagacaaatc agaggaagta3120caaaaaattg ctgaactttg cgccaattca attaaggatt atgagctcca gctggcctca3180tacacctcag gactggaaac tctgctgaac atacctatca agaggaccat gattcagtcc3240ccttctgggg tgattctgca agaggctgca gatgttcatg ctcggtacat tgaactactt3300acaagatctg gagactatta caggttctta agtgagatgc tgaagagttt ggaagatctg3360aagctgaaaa ataccaagat cgaagttttg gaagaggagc tcagactggc ccgagatgcc3420aactcggaaa actgtaataa gaacaaattc ctggatcaga acctgcagaa ataccaggca3480gagtgttccc agttcaaagc gaagcttgcg agcctggagg agctgaagag acaggctgag3540ctggatggga agtcggctaa gcaaaatcta gacaagtgct acggccaaat aaaagaactc3600aatgagaaga tcacccgact gacttatgag attgaagatg aaaagagaag aagaaaatct3660gtggaagaca gatttgacca acagaagaat gactatgacc aactgcagaa agcaaggcaa3720
tgtgaaaagg agaaccttgg ttggcagaaa ttagagtctg agaaagccat caaggagaag3780gagtacgaga ttgaaaggtt gagggttcta ctgcaggaag aaggcacccg gaagagagaa3840tatgaaaatg agctggcaaa ggtaagaaac cactataatg aggagatgag taatttaagg3900aacaagtatg aaacagagat taacattacg aagaccacca tcaaggagat atccatgcaa3960aaagaggatg attccaaaaa tcttagaaac cagcttgata gactttcaag ggaaaatcga4020gatctgaagg atgaaattgt caggctcaat gacagcatct tgcaggccac tgagcagcga4080aggcgagctg aagaaaacgc ccttcagcaa aaggcctgtg gctctgagat aatgcagaag4140aagcagcatc tggagataga actgaagcag gtcatgcagc agcgctctga ggacaatgcc4200cggcacaagc agtccctgga ggaggctgcc aagaccattc aggacaaaaa taaggagatc4260gagagactca aagctgagtt tcaggaggag gccaagcgcc gctgggaata tgaaaatgaa4320ctgagtaagg taagaaacaa ttatgatgag gagatcatta gcttaaaaaa tcagtttgag4380accgagatca acatcaccaa gaccaccatc caccagctca ccatgcagaa ggaagaggat4440accagtggct accgggctca gatagacaat ctcacccgag aaaacaggag cttatctgaa4500gaaataaaga ggctgaagaa cactctaacc cagaccacag agaatctcag gagggtggaa4560gaagacatcc aacagcaaaa ggccactggc tctgaggtgt ctcagaggaa acagcagctg4620gaggttgagc tgagacaagt cactcagatg cgaacagagg agagcgtaag atataagcaa4680tctcttgatg atgctgccaa aaccatccag gataaaaaca aggagataga aaggttaaaa4740caactgatcg acaaagaaac aaatgaccgg aaatgcctgg aagatgaaaa cgcgagatta4800caaagggtcc agtatgacct gcagaaagca aacagtagtg cgacggagac aataaacaaa4860ctgaaggttc aggagcaaga actgacacgc ctgaggatcg actatgaaag ggtttcccag4920gagaggactg tgaaggacca ggatatcacg cggttccaga actctctgaa agagctgcag4980ctgcagaagc agaaggtgga agaggagctg aatcggctga agaggaccgc gtcagaagac5040tcctgcaaga ggaagaagct ggaggaagag ctggaaggca tgaggaggtc gctgaaggag5100caagccatca aaatcaccaa cctgacccag cagctggagc aggcatccat tgttaagaag5160aggagtgagg atgacctccg gcagcagagg gacgtgctgg atggccacct gagggaaaag5220cagaggaccc aggaagagct gaggaggctc tcttctgagg tcgaggccct gaggcggcag5280ttactccagg aacaggaaag tgtcaaacaa gctcacttga ggaatgagca tttccagaag5340gcgatagaag ataaaagcag aagcttaaat gaaagcaaaa tagaaattga gaggctgcag5400tctctcacag agaacctgac caaggagcac ttgatgttag aagaagaact gcggaacctg5460aggctggagt acgatgacct gaggagagga cgaagcgaag cggacagtga taaaaatgca5520accatcttgg aactaaggag ccagctgcag atcagcaaca accggaccct ggaactgcag5580gggctgatta atgatttaca gagagagagg gaaaatttga gacaggaaat tgagaaattc5640caaaagcagg ctttagaggc atctaatagg attcaggaat caaagaatca gtgtactcag5700gtggtacagg aaagagagag ccttctggtg aaaatcaaag tcctggagca agacaaggca5760
aggctgcaga ggctggagga tgagctgaat cgtgcaaaat caactctaga ggcagaaacc5820agggtgaaac agcgcctgga gtgtgagaaa cagcaaattc agaatgacct gaatcagtgg5880aagactcaat attcccgcaa ggaggaggct attaggaaga tagaatcgga aagagaaaag5940agtgagagag agaagaacag tcttaggagt gagatcgaaa gactccaagc agagatcaag6000agaattgaag agaggtgcag gcgtaagctg gaggattcta ccagggagac acagtcacag6060ttagaaacag aacgctcccg atatcagagg gagattgata aactcagaca gcgcccatat6120gggtcccatc gagagaccca gactgagtgt gagtggaccg ttgacacctc caagctggtg6180tttgatgggc tgaggaagaa ggtgacagca atgcagctct atgagtgtca gctgatcgac6240aaaacaacct tggacaaact attgaagggg aagaagtcag tggaagaagt tgcttctgaa6300atccagccat tccttcgggg tgcaggatct atcgctggag catctgcttc tcctaaggaa6360aaatactctt tggtagaggc caagagaaag aaattaatca gcccagaatc cacagtcatg6420cttctggagg cccaggcagc tacaggtggt ataattgatc cccatcggaa tgagaagctg6480actgtcgaca gtgccatagc tcgggacctc attgacttcg atgaccgtca gcagatatat6540gcagcagaaa aagctatcac tggttttgat gatccatttt caggcaagac agtatctgtt6600tcagaagcca tcaagaaaaa tttgattgat agagaaaccg gaatgcgcct gctggaagcc6660cagattgctt cagggggtgt agtagaccct gtgaacagtg tctttttgcc aaaagatgtc6720gccttggccc gggggctgat tgatagagat ttgtatcgat ccctgaatga tccccgagat6780agtcagaaaa actttgtgga tccagtcacc aaaaagaagg tcagttacgt gcagctgaag6840gaacggtgca gaatcgaacc acatactggt ctgctcttgc tttcagtaca gaagagaagc6900atgtccttcc aaggaatcag acaacctgtg accgtcactg agctagtaga ttctggtata6960ttgagaccgt ccactgtcaa tgaactggaa tctggtcaga tttcttatga cgaggttggt7020gagagaatta aggacttcct ccagggttca agctgcatag caggcatata caatgagacc7080acaaaacaga agcttggcat ttatgaggcc atgaaaattg gcttagtccg acctggtact7140gctctggagt tgctggaagc ccaagcagct actggcttta tagtggatcc tgttagcaac7200ttgaggttac cagtggagga agcctacaag agaggtctgg tgggcattga gttcaaagag7260aagctcctgt ctgcagaacg agctgtcact gggtataatg atcctgaaac aggaaacatc7320atctctttgt tccaagccat gaataaggaa ctcatcgaaa agggccacgg tattcgctta7380ttagaagcac agatcgcaac cggggggatc attgacccaa aggagagcca tcgtttacca7440gttgacatag catataagag gggctatttc aatgaggaac tcagtgagat tctctcagat7500ccaagtgatg ataccaaagg attttttgac cccaacactg aagaaaatct tacctatctg7560caactaaaag aaagatgcat taaggatgag gaaacagggc tctgtcttct gcctctgaaa7620gaaaagaaga aacaggtgca gacatcacaa aagaataccc tcaggaagcg tagagtggtc7680atagttgacc cagaaaccaa taaagaaatg tctgttcagg aggcctacaa gaagggccta7740attgattatg aaaccttcaa agaactgtgt gagcaggaat gtgaatggga agaaataacc7800atcacgggat cagatggctc caccagggtg gtcctggtag atagaaagac aggcagtcag7860
tatgatattc aagatgctat tgacaagggc cttgttgaca ggaagttctt tgatcagtac7920cgatccggca gcctcagcct cactcaattt gctgacatga tctccttgaa aaatggtgtc7980ggcaccagca gcagcatggg cagtggtgtc agcgatgatg tttttagcag ctcccgacat8040gaatcagtaa gtaagatttc caccatatcc agcgtcagga atttaaccat aaggagcagc8100tctttttcag acaccctgga agaatcgagc cccattgcag ccatctttga cacagaaaac8160ctggagaaaa tctccattac agaaggtata gagcggggca tcgttgacag catcacgggt8220cagaggcttc tggaggctca ggcctgcaca ggtggcatca tccacccaac cacgggccag8280aagctgtcac ttcaggacgc agtctcccag ggtgtgattg accaagacat ggccaccagc8340gtgaagcctg ctcagaaagc cttcataggc ttcgagggtg tgaagggaaa gaagaagatg8400tcagcagcag aggcagtgaa agaaaaatgg ctcccgtatg aggctggcca gcgcttcctg8460gagttccagt acctcacggg aggtcttgtt gacccggaag tgcatgggag gataagcacc8520gaagaagcca tccggaaggg gttcatagat ggccgcgccg cacagaggct gcaagacacc8580agcagctatg ccaaaatcct gacctgcccc aaaaccaaat taaaaatatc ctataaggat8640gccataaatc gctccatggt agaagatatc actgggctgc gccttctgga agccgcctcc8700gtgtcgtcca agggcttacc cagcccttac aacatgtctt cggctccggg gtcccgctcc8760ggctcccgct cgggatctcg ctccggatct cgctccgggt cccgcagtgg gtcccggaga8820ggaagctttg acgccacagg gaattcttcc tactcttatt cctactcatt tagcagtagt8880tctattgggc actagtagtc agttgggagt ggttgctata ccttgacttc atttatatga8940atttccactt tattaaataa tagaaaagaa aatcccggtg cttgcagtag agtgatagga9000cattctatgc ttacagaaaa tatagccatg attgaaatca aatagtaaag gctgttctgg9060ctttttatct tcttagctca tcttaaataa gcagtacact tggatgcagt gcgtctgaag9120tgctaatcag ttgtaacaat agcacaaatc gaacttagga tttgtttctt ctcttctgtg9180tttcgatttt tgatcaattc tttaattttg gaagcctata atacagtttt ctattcttgg9240agataaaaat taaatggatc actgatattt tagtcattct gcttctcatc taaatatttc9300catattctgt attaggagaa aattaccctc ccagcaccag cccccctctc aaacccccaa9360cccaaaacca agcattttgg aatgagtctc ctttagtttc agagtgtgga ttgtataacc9420catatactct tcgatgtact tgtttggttt ggtattaatt tgactgtgca tgacagcggc9480aatcttttct ttggtcaaag ttttctgttt attttgcttg tcatattcga tgtactttaa9540ggtgtcttta tgaagtttgc tattctggca ataaactttt agactttt 9588<210>33<211>366<212>DNA<213>人<220>
<221>misc_特征<222>(351)..(351)<223>任意堿基
<400>33gaagtgccat ttatatttat acaaaaatat tacataattc agttagtatt ggtgacataa 60tttagttagt atgggtgata taatggtcat aatttttagc atctaataaa gatcttttta 120tgagtcccat ataaaatatg tgaacaaagc aatcttgtca taagatttgt gatgatttag 180gagaaagtac tttgagataa tttttttctg tctctttgtg aactctctca acagtagttc 240tctttagatt agagccagca ggtcggccat aacagttttc ttcaaatttg ggcaacagtt 300atacaaatgc ttgaatttca agacaacata ttaaagggtc tatgaactgg naatctaacc 360tgggtt366<210>34<211>1466<212>DNA<213>人<400>34agccccaagc ttaccacctg cacccggaga gctgtgtgtc accatgtggg tcccggttgt 60cttcctcacc ctgtccgtga cgtggattgg tgctgcaccc ctcatcctgt ctcggattgt 120gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg cctctcgtgg 180cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag ctgcccactg 240catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc ctgaagacac 300aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata tgagcctcct 360gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc tgctccgcct 420gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca cccaggagcc 480agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag aggagttctt 540gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg tgtgtgcgca 600agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga cagggggcaa 660aagcacctgc tcgggtgatt ctgggggccc acttgtctgt aatggtgtgc ttcaaggtat 720cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt acaccaaggt 780ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctgag cacccctatc 840aaccccctat tgtagtaaac ttggaacctt ggaaatgacc aggccaagac tcaagcctcc 900ccagttctac tgacctttgt ccttaggtgt gaggtccagg gttgctagga aaagaaatca 960gcagacacag gtgtagacca gagtgtttct taaatggtgt aattttgtcc tctctgtgtc1020ctggggaata ctggccatgc ctggagacat atcactcaat ttctctgagg acacagatag1080gatggggtgt ctgtgttatt tgtggggtac agagatgaaa gaggggtggg atccacactg1140agagagtgga gagtgacatg tgctggacac tgtccatgaa gcactgagca gaagctggag1200gcacaacgca ccagacactc acagcaagga tggagctgaa aacataaccc actctgtcct1260ggaggcactg ggaagcctag agaaggctgt gagccaagga gggagggtct tcctttggca1320tgggatgggg atgaagtaag gagagggact ggaccccctg gaagctgatt cactatgggg1380
ggaggtgtat tgaagtcctc cagacaaccc tcagatttga tgatttccta gtagaactca1440cagaaataaa gagctgttat actgtg 1466<210>35<211>187<212>DNA<213>人<400>35gatctggtgc attccggtcg acactctcgt ttatttggac tgtaagtctg acctctatga 60ataattactt cagcccctga gtgctcccgg gccaagctcc ttggccaaac tttcacctta 120gcttctgata agtcttgggc caagctaagc agcatctatc aatcatccct tcagctcctg 180attgatc 187<210>36<211>2913<212>DNA<213>人<400>36actgggtacc gaggactggg tgtgtttaag gcagacagcc aggtgaggat cccagctact 60ggggcctgct gtcatctcct gggagtaccc gggggtcagg agcctagggg actcttgcac 120ttcacatcca gccatgctaa ttacactttt tggcaaagga aacagctagg agcagtttct 180ttcactccta cagccccgtt ttctcagtgt ttagacctcg aattattact gggctagagg 240gaaggcagcc tctgaagtgt ggcaggagga ggggaagtct gcctgcatct tggtgtgtct 300gtcagatgcc agcactaata acctggcttc tgtgaggcct gtcagtgctc tcaggaatga 360aaggggaccc ctgagaggtg ctcagtacca gcaggctgtg aatgctctct acccaccacc 420ctcacctcct cgttaaagat ggtgctacct gccacacagc agacatctgg tcgctgcaca 480cccgaaagac cccaaggcag tctgcccctt gtccagccac acgccagcac ccaccctcct 540ggcccctgcc tcggcctccc cagaccagct gcacccagcc cccaacacgc accccttctc 600cagatgtgtg cagggcctca ttttgcagag caaagacaga tgtttcagcc acacgcttta 660ttaacttcta aaacctgtgc tcaggacact cttcaacagt catgaaaagt ttgatcactt 720gccacagtca ggacctttgt gtggggctct gatctgatgt tcggtctcat catctcccaa 780accagcagtc gtttgtaccc caaccctctg ctcaggggct cataccccca aatgattttc 840ctgatttatg tatttcccta caaagggctt tctataccta gcatctgcct ccagcatgag 900aagggggaat aggtgagacc catttgccag tagcagacgg ggaccctggg gagaaaatgg 960cagagcctgt tggagactcc ctgtctccag ctgaccagcc aatgggattc ctcttccctc1020cactgtctcc cacaaagtag aagaatcctg gtacatttag cccatgagcc tggcacagat1080ccctatctag acatgaggcc ctttagacat gactttggca ttgaccagcc tgttggcaat1140gggtcgggga ggcagagggg atgctcacac cagtaattct catcccctga atgcttggga1200tcacctgggg agagttcaca aaatactggt gcaggggtcc cacctctgat gatgctgagt1260
ggtgggtctg gggtgtggcc caggcatcat gatgtttcag gcccccaggt gacttcttag1320gcagcccagc taagccccta gagccttgca atttccccca aatgacctca gagggcccga1380tttgagggaa atgcctaact tcaggggccg taagaatccc ccagggagca tgtgaaatgc1440agataccagg cccaccccca gagatgagct gaggtgggtc aggggtgaag tgcagggatc1500agtgtttttc acaagctcca tacctccagg aaatggtgtt gtggttgggc ccgtagaaaa1560cattctgaga gtcctgttgc ctgtgccttg gtgcacgtgg ggtggaatcc cagtggccct1620gccttgagga ggatgtgcat taacgtggta ggggagacag agacagctcc acctgccccc1680tgtcccaccg gggacctcca aaaacttcat ggatgttaga gcaagcagcc atgctgcagc1740agaggatgag gctggcggat ttagtaagag ccctctgtgt ttgggctgag ttctttctct1800agttgccctg tcatctggcc tctggataac ccacctctcc tccctcatcc taaaattaca1860gatggcgaaa gacggccaca tttagtgaga cccctaaggt cctccaacta gggtgggtcc1920acagtggccc ctggtgcatg gaccacacac tctcttccct cctctggctc aggactacgg1980tctgaaatta gggagatatg aatgtctttc ttgaaaactt ctcttcccag tcttcccact2040ttgcttgggg gtccttggtc aaggccagct ttggactagg gcttgttgcg actaccagct2100gtctcatttt gctgtactgc aaactcaggc ttggttccaa gcttatgggg gccctgtcct2160tcccctagta gggtttgttt tggggtcaca tctggtcata cccttcagag agctcttccc2220cagcctctac atcagggaga gaggtaggta gggaggagca ttcaaggatt agaagaagga2280ctaaagtaca acagccttgg aggaactgcc aggaactaag ggcgagcact ggagaaggca2340acctgggacc ccctgcgctt ctgagcagga agaccaagac cttcaggggc cctaagcact2400gaaaacatca ttcctcatcc ccaagccctg gcatccccct gttcttctaa aataattctt2460ttctaggtat ttctgattgc aaaattctgg atgggttcat ccaagctgac ctttgctgtt2520ttttcccttc ccaacaaggc ctcacttttt ggagccacct tagttggtgc ctaggcagag2580gggcagtcag cagtggttat caggatcctg gctctatggg ttgccttcct cctggtctgt2640aaagcccctg caggcaggga cttcttagat agctgcttcc ttagggcatg gcatgtggtg2700ggtggttaat gaatggaaga gagggaatga gtgatcaagg gagggaggag ggagtggagt2760ggagatttct catcctttcc tgttaattta tgacatcctc ctgcctatga gtccttgact2820ctggagtttt acaaagcagt cacatttcaa ataaaagtct gggaaagcaa cacatcatcg2880ccaactttta attttgctaa ataaggatat tag 2913<210>37<211>1466<212>DNA<213>人<400>37agccccaagc ttaccacctg cacccggaga gctgtgtgtc accatgtggg tcccggttgt 60cttcctcacc ctgtccgtga cgtggattgg tgctgcaccc ctcatcctgt ctcggattgt 120gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg cctctcgtgg 180
cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag ctgcccactg 240catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc ctgaagacac 300aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata tgagcctcct 360gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc tgctccgcct 420gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca cccaggagcc 480agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag aggagttctt 540gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg tgtgtgcgca 600agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga cagggggcaa 660aagcacctgc tcgggtgatt ctgggggccc acttgtctgt aatggtgtgc ttcaaggtat 720cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt acaccaaggt 780ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctgag cacccctatc 840aaccccctat tgtagtaaac ttggaacctt ggaaatgacc aggccaagac tcaagcctcc 900ccagttctac tgacctttgt ccttaggtgt gaggtccagg gttgctagga aaagaaatca 960gcagacacag gtgtagacca gagtgtttct taaatggtgt aattttgtcc tctctgtgtc1020ctggggaata ctggccatgc ctggagacat atcactcaat ttctctgagg acacagatag1080gatggggtgt ctgtgttatt tgtggggtac agagatgaaa gaggggtggg atccacactg1140agagagtgga gagtgacatg tgctggacac tgtccatgaa gcactgagca gaagctggag1200gcacaacgca ccagacactc acagcaagga tggagctgaa aacataaccc actctgtcct1260ggaggcactg ggaagcctag agaaggctgt gagccaagga gggagggtct tcctttggca1320tgggatgggg atgaagtaag gagagggact ggaccccctg gaagctgatt cactatgggg1380ggaggtgtat tgaagtcctc cagacaaccc tcagatttga tgatttccta gtagaactca1440cagaaataaa gagctgttat actgtg 1466<210>38<211>462<212>DNA<213>人<220>
<221>misc_特征<222>(197)..(197)<223>任意堿基<220>
<221>misc_特征<222>(116)..(116)<223>任意堿基<220>
<221>misc_特征<222>(334)..(334)<223>任意堿基<220>
<221>misc_特征<222>(402)..(402)<223>任意堿基<220>
<221>misc_特征<222>(429)..(429)<223>任意堿基<220>
<221>misc_特征<222>(438)..(438)<223>任意堿基<220>
<221>misc_特征<222>(443)..(443)<223>任意堿基<220>
<221>misc_特征<222>(459)..(459)<223>任意堿基<400>38taaggtttta taattatttt tatttttctt ttcttttttt tttatggctt ggatgacact 60ttattttcag atccaatact agaagttgtt tccatgttca cattttcctt cctggnttaa 120aaaaaagagt tgtatttttt ttttttgctt tttttaaatt atactttaag ttttagggta 180catgtgcaca acgcagnggt tagctacata tgtatacatg tgccatgttg gcgtgctgca 240tccagtaact cgtcatttaa cattaggtat atctccaaat gctatccttc cccccattgt 300atttttcata gcttaaaaat cattgacata ggantaattc caactaaagt acggtattaa 360atccctgggg gaataaattt tgtcttaaca agggtaaggt tngtgaaaag gatggttttg 420tcacagggna aaaggganat ccncccattt taaaacccnc ct462<210>39<211>1490<212> DNA<213>人<400>39ctcgtgcccc ccacggaggg gactgctctc ccccgctgca tcctttctgt gaggtacctt 60acccacctca gcacctgaga gggtgaaata gaattctaac ctcgacattc gggaagtgtt 120tttgagaagt ctcggtcggt aagggaagtc ttccaagtcc gtgcagcact aacgtattgg 180cacctgcctc ctcttcggcc accccccaga tgaggcagct gtgactgtgt caagggaagc 240cacgactctg accatagtct tctctcagct tccactgccg tctccacagg aaacccagaa 300gttctgtgaa caagtccatg ctgccatcaa ggcatttatt gcagtgtact atttgcttcc 360aaaggatcag gggatcaccc tgagaaagct ggtacggggc gccaccctgg acatcgtgga 420tggcatggct cagctcatgg aagtactttc cgtcactcca actcagagcc ctgagaacaa 480
tgaccttatt tcctacaaca gtgtctgggt tgcgtgccag cagatgcctc agataccaag 540agataacaaa gctgcagctc ttttgatgct gaccaagaat gtggattttg tgaaggatgc 600acatgaagaa atggagcagg ctgtggaaga atgtgaccct tactctggcc tcttgaatga 660tactgaggag aacaactctg acaaccacaa tcatgaggat gatgtgttgg ggtttcccag 720caatcaggac ttgtattggt cagaggacga tcaagagctc ataatcccat gccttgcgct 780ggtgagagca tccaaagcct gcctgaagaa aattcggatg ttagtggcag agaatgggaa 840gaaggatcag gtggcacagc tggatgacat tgtggatatt tctgatgaaa tcagccctag 900tgtggatgat ttggctctga gcatatatcc acctatgtgt cacctgaccg tgcgaatcaa 960ttctgcgaaa cttgtatctg ttttaaagaa ggcacttgaa attacaaaag caagtcatgt1020gacccctcag ccagaagata gttggatccc tttacttatt aatgccattg atcattgcat1080gaatagaatc aaggagctca ctcagagtga acttgaatta tgacttttca ggctcatttg1140tactctcttc ccctctcatc gtcatggtca ggctctgata cctgctttta aaatggagct1200agaatgcttg ctggattgaa agggagtgcc tatctatatt tagcaagaga cactattacc1260aaagattgtt ggttaggcca gattgacacc tatttataaa ccatatgcgt atatttttct1320gtgctatata tgaaaaataa ttgcatgatt tctcattcct gagtcatttc tcagagattc1380ctaggaaagc tgccttattc tctttttgca gtaaagtatg ttgttttcat tgtaaagatg1440ttgatggtct caataaaatg ctaacttgcc agtgattaaa aaaaaaaaaa 1490<210>40<211>1677<212> DNA<213>人<400>40cttgacccta tttatagtgg ctctaaaggt ggtgttatta tgttttctag agcacttcga 60ttatacaaac gtcaaggaat ccgagttaat gtgctttgcc ctgagtttgt tgaaacagac 120atgggcacaa tgatcggtcc caaattcctt agtatgatgg ggggctttgt acctatggaa 180atggtggtga aaggtgcttt tgagctcatc actgatgaga ataaagccgg cgattgccta 240tggattacta atcggcgagg tcttgagtac tggcccaccc catcagaaga agcaaagtac 300ttgctgcgtt ctacacgttc caggagaaga actgaataca aagctccacc aattaaacta 360cctgagagtt ttgagaaaat agttgttcag accttgactc acaactttcg gaatgctacc 420agtgtagtaa gagcaccact gagattacct atcaaaccaa actatgttct tgtgaagata 480atctatgctg gtgtaaatgc tagtgatgta aattttagct caggtcgcta ttttggtggc 540aataacagtg acactgcatc ccgtcttccg tttgatgcag gatttgaggc tgtgggagta 600attgcagcag ttggggattc tgttactgac ttgaaagttg gcatgccttg tgcgttcatg 660acttttggag gctatgctga atttacaatg attccttcga aatacgccct tccaatgcct 720agaccagaac cggaaggtgt tgccatgctt acatcaggat taacagcttc aattgctcta 780gaaaaggcag gacagatgga atctggaaaa gtggtccttg ttactgctgc ggcaggagga 840
actggtcagt ttgctgttca gcttgcaaaa ttagctggta ataccgtggt tgccacttgt 900ggaggtgggg caaaggccaa gcttctgaaa gaattgggag tcgacagagt catagactat 960cacagtgaag atataaaaac ggttctaagg aaagagttcc cgaaaggtat tgatatcatc1020tacgaatctg ttggtgggga catgttaaag ttgtgcttgg atgctttggc agtccatgga1080cgactcattg tcattggcat gatttctcag tatcaaggag aaaatggttg gacgccatca1140aaatatcctg gactatgtga gaagctcttg tcaaagagtc aaactgtggc tggctttttc1200ctggtgcaat atagtcacat gtaccaagaa caccttaaca agttatttga cctttactct1260tccggaaaac taaaggttgc tgtggatcca aagagattta taggccttca ttctgttgct1320gatgctgttg agtatctcca ttcaggcaaa agcgttggga aggtggttgt ctgcgtggac1380ccgaccttcg gtcatcaagt agccaaatta tgaatgaaca cggtgtcaaa tacagaaaga1440agtgaagttt tcaattctta gtctagagat tgttctcgaa tgttactgaa aatagctgct1500agaccagtgc tggaatattt attctcaatg ctttttcaat tttggattac ttgaaagaat1560aatccattta tgtataccat gtttatgttt acactataca acaactatga gcagaagaaa1620gcgagatatc tacaaaataa attataatcc tttcatttta aaaaaaaaaa aaaaaaa 1677<210>41<211>1330<212>DNA<213>人<400>41atggcgcagt gggacagctt cactgatcaa caggaggaca ctgatagctg ttcagaatct 60gtgaagtttg atgctcgctc caatacagct ttgcttcccc caaatcctaa aaatggccct 120ccacttcaag aaaagctgaa atccttcaaa gctgcactga ttgcccttta tctccttgtg 180tttgctgttc tcattcctat catcgcaata atggcagctc aactcctgaa gtgggaaatg 240aagaattgca cagttggttc aattaatgca aacagtgtat cctccagtct cctgggaaga 300ggaaatgaca gtgaagatga agtgagattt cgagaagttg ttatggaaca cattagcaag 360atggagaaaa gaatccaata tatttcagat actgaagaaa atctcgtaga ttcagagcat 420tttcaaaatt tcagtgtgac aactgatcaa cgatttgctg atgttcttct ccaactaagt 480accttggttc ccacagtcca gggacatggg aatgccgtag atgaaatcac caggtcctta 540ataagtctga ataccacgct gcttgatttg cacctctatg tagaaacact gaatgtcaaa 600ttccaggaga atacacttaa agggcaagag gaaatcagca aattaaagga gcgtgtgcac 660aatgcatcag cagaaattat gtctatgaaa gaagaacaag tgcatttgga acaggaaata 720aaaagagaag tgaaagtcct gaataacatc actaatgatc tcaggctgaa agattgggaa 780cattctcaga cgttgagaaa tatcacttta attcaaggtc ctcctggacc cccaggagaa 840aaaggagata gaggtccaac cggagaaagt ggtccaccag gcgtttcagg tccagtaggt 900cctccaggtc ttaagggtga tcgaggatct attggctttc cgggaagtcg aggatatcca 960ggacaatcag ggaagactgg gaggacagga tatcctggac caaaaggcca aaagggagaa1020
aaaggcagtg gaagcatcct gactccttct gcgactgtcc gactggttgg tggccgtggc1080cctcatgagg gtagagtgga gatattgcac aatggacagt ggggcacagt ttgtgatgat1140cactgggaac tgcgtgccgg gcaggttgtc tgcaggagct tgggataccg aggtgttaag1200agtgtgcaca agaaagctta ttttggacaa ggtactggtc ccatttggct gaatgaagta1260ccctgtttgg ggatggagtc atccattgaa gagtgcaaaa tcagacagtg gggcgtgaga1320gtctgttcac 1330<210>42<211>431<212>DNA<213>人<220>
<221>misc_特征<222>(97)..(97)<223>任意堿基<220>
<221>misc_特征<222>(347)..(347)<223>任意堿基<220>
<221>misc_特征<222>(349)..(349)<223>任意堿基<220>
<221>misc_特征<222>(361)..(361)<223>任意堿基<220>
<221>misc_特征<222>(362)..(362)<223>任意堿基<220>
<221>misc_特征<222>(363)..(363)<223>任意堿基<220>
<221>misc_特征<222>(401)..(401)<223>任意堿基<220>
<221>misc_特征<222>(428)..(428)<223>任意堿基<400>42ctttttatat ttattttcat cgctacacaa acatttttta ggagtttgat tctacctcca 60
ttttggttag atatacaaac tctaccccat gagggantgt atggtgtatt tctagattta 120gcaacaattt tcttgaaaaa tgtacaatac tatagaaaaa tgaagatagt aaataccagg 180tataagttaa taacagtgtt tcttttgttc agtaataatg aactgtgtac tagcactgaa 240ctttaggccc tcctatttgc gtattttctg tttgtatatt tttaaataga ggaattgtga 300ttataatatt attattttgg aatatcctaa atcataaatt caaaacntna tttagttttt 360nnnttttttt tttaagatgg agtcccgctt tgtcccaggc nggagtgcag tggcatgatc 420tcagctcnct g 431<210>43<211>669<212>DNA<213>人<220>
<221>misc_特征<222>(641)..(641)<223>任意堿基<400>43ttcttttgga aaaccaaaca tgctttattt catttttttc acaatttatt taaacatctc 60acatatacaa aataggtaca atttaatttt tctgcttgcc caagaaacaa agcttctgtg 120gaaccatgga agaagatgaa aatgagactg gcaaagaaca aatgctgaat ctgaagaaga 180ggacaacttt gggcaaataa tctgcatact tttaattggg aataagatgg aaaatatgaa 240tgctaaatca aattttttaa aaaatacacc acacgataca actcaataca ggagtatttc 300ttctcaaatt cttctagcac catcaacatt cttcaagtat ctgaaatact attaattagc 360acctttgtat tatgaacaaa acaaaacaag gacctcagtt catctctgtc taggtcagca 420cctaacaatg tggatcacac tcatgggaaa gtgttttgag gtagtttaaa cctttggaag 480tttgggtttt aaacttccct ctgtggaaga tattcaaaag ccacaagtgg tgcaaatgtt 540tatggttttt atttttcaat ttttattttg gttttcttac aaaggttgac atttttcata 600acaggtgtaa gagtgttgaa aaaaaaattt caatttttgg ngggaacggg ggaaggagtt 660aatgaaact 669<210>44<211>287<212>DNA<213>人<400>44gccggagagt ctacaatgtt acccagcatg ctgttggcat tgttgtaaac aaacaagtta 60agggcaagat tcttgccaag agaattaatg tgcgtattga gcacattaag cactctaaga 120gccgagatag cttcctgaaa cgtgtgaagg aaaatgatca gaaaaagaaa gaagccaaag 180agaaaggtac ctgggttcaa ctaaagcgcc acgctgctcc acccagagaa gcacactttg 240tgagaaccaa tgggaaggag cctgagctgc tggaacctat tccctat 287
<210>45<211>383<212>DNA<213>人<220>
<221>misc_特征<222>(147)..(147)<223>任意堿基<220>
<221>misc_特征<222>(309)..(309)<223>任意堿基<220>
<221>misc_特征<222>(349)..(349)<223>任意堿基<220>
<221>misc_特征<222>(365)..(365)<223>任意堿基<220>
<221>misc_特征<222>(372)..(372)<223>任意堿基<220>
<221>misc_特征<222>(380)..(380)<223>任意堿基<400>45ggaacggaaa aggagaattc aagtgtgacc ctcatgaggc aacgtgttat gatgatggga 60agacatacca cgtaggagaa cagtggcaga aggaatatct cggtgccatt tgctcctgca 120catgctttgg aggccagcgg ggctcgnctt gtgacaactg ccgcagacct ggggggtgaa 180cccagtcccg aaggcactac tggccagtcc tacaaccagt attcttcaga gataccattc 240agagaacaaa cactaatgtt taatttgccc aatttgagtg cttcatgcct tttaggatgt 300tacaggctng acagagaagg ttttcccgag gagttaaatc atctttttnc catttcccga 360ggggncaagg cntgtttttn ttt 383<210>46<211>523<212>DNA<213>人<400>46cagaggggca gggcggacgg ctaggagttc aagaaacatc ctggtctgag ggaaaggctg 60cagctgcacc gccatgaata agcttttcag cttctggaag aggaagaatg agacccgcag 120ccagggctac aaccttcgag aaaaggattt aaagaaactt cacagagctg cttcagtcgg 180
ggatttgaag aagctgaagg aataccttca gatcaagaaa tatgatgtaa atatgcagga 240ctatgaatac agaacacctt tgcacctagc ctgtgctaat ggacatacag atgttgtact 300tttcctaatt gagcaacaat gcaagataaa tgtccgggat agtgaaaaca aatccccatt 360gattaaggca gtacagtgtc aaaatgagga ttgtgctact attctgctaa actttggtgc 420agacccagat ctgagggata ttcgttataa tactgttctt cactatgctg tttgtggtca 480aagtttgtca ttagttgaaa aactgcttga atacgaagct gat 523<210>47<211>390<212>DNA<213>人<400>47tccaaggtca tggcaaaaca tctgaagttc atcgccagga ctgtgatggt acaggaaggg 60aacgtggaaa gcgcatacag gaccctaaac agaatcctca ctatggatgg gctcattgag 120gacattaagc atcggcggta ttatgagaag ccatgccgcc gcgacagagg gaaagctatg 180aaaggtgccg gcggatctac aacatggaaa tggctcgcaa gatcaacttc ttgatgcgaa 240agaatcgggc agatccgtgg cagggctgct gaggcctgtg ggtgggacac cagtgcgaaa 300ccctcatcca gttttctctc catctctttt ctttgtacaa tcccatttcc tattaccatt 360ctctgcaata aactcaaatc acatgtctgc 390<210>48<211>669<212>DNA<213>人<220>
<221>misc_特征<222>(641)..(641)<223>任意堿基<400>48ttcttttgga aaaccaaaca tgctttattt catttttttc acaatttatt taaacatctc 60acatatacaa aataggtaca atttaatttt tctgcttgcc caagaaacaa agcttctgtg 120gaaccatgga agaagatgaa aatgagactg gcaaagaaca aatgctgaat ctgaagaaga 180ggacaacttt gggcaaataa tctgcatact tttaattggg aataagatgg aaaatatgaa 240tgctaaatca aattttttaa aaaatacacc acacgataca actcaataca ggagtatttc 300ttctcaaatt cttctagcac catcaacatt cttcaagtat ctgaaatact attaattagc 360acctttgtat tatgaacaaa acaaaacaag gacctcagtt catctctgtc taggtcagca 420cctaacaatg tggatcacac tcatgggaaa gtgttttgag gtagtttaaa cctttggaag 480tttgggtttt aaacttccct ctgtggaaga tattcaaaag ccacaagtgg tgcaaatgtt 540tatggttttt atttttcaat ttttattttg gttttcttac aaaggttgac atttttcata 600acaggtgtaa gagtgttgaa aaaaaaattt caatttttgg ngggaacggg ggaaggagtt 660aatgaaact 669
<210>49<211>431<212>DNA<213>人<220>
<221>misc_特征<222>(97)..(97)<223>任意堿基<220>
<221>misc_特征<222>(347)..(347)<223>任意堿基<220>
<221>misc_特征<222>(349)..(349)<223>任意堿基<220>
<221>misc_特征<222>(361)..(361)<223>任意堿基<220>
<221>misc_特征<222>(362)..(362)<223>任意堿基<220>
<221>misc_特征<222>(363)..(363)<223>任意堿基<220>
<221>misc_特征<222>(401)..(401)<223>任意堿基<220>
<221>misc_特征<222>(428)..(428)<223>任意堿基<400>49ctttttatat ttattttcat cgctacacaa acatttttta ggagtttgat tctacctcca 60ttttggttag atatacaaac tctaccccat gagggantgt atggtgtatt tctagattta 120gcaacaattt tcttgaaaaa tgtacaatac tatagaaaaa tgaagatagt aaataccagg 180tataagttaa taacagtgtt tcttttgttc agtaataatg aactgtgtac tagcactgaa 240ctttaggccc tcctatttgc gtattttctg tttgtatatt tttaaataga ggaattgtga 300ttataatatt attattttgg aatatcctaa atcataaatt caaaacntna tttagttttt 360nnnttttttt tttaagatgg agtcccgctt tgtcccaggc nggagtgcag tggcatgatc 420
tcagctcnct g 43權(quán)利要求
1.一種評(píng)估結(jié)腸直腸癌狀況的方法，包括在選自Seq.ID.No.42-49的基因組合中鑒定每個(gè)基因(相對(duì)于正常種群的相同基因的表達(dá))的差異調(diào)節(jié)。
2.權(quán)利要求1的方法，其中調(diào)節(jié)基因表達(dá)中存在至少2倍差異。
3.權(quán)利要求1的方法，其中表示差異調(diào)節(jié)的p值低于.05。
4.權(quán)利要求1的方法，進(jìn)一步包括用于非遺傳基礎(chǔ)的結(jié)腸直腸癌診斷。
5.權(quán)利要求的方法，其中非遺傳基礎(chǔ)的癌癥標(biāo)記選自癌胚性抗原、CA19-9、CA125、CK-BB和鳥苷酸環(huán)化酶C。
6.一種診斷框架，包括選自Seq.ID.No.42-49的基因組合中分離的核酸序列、其互補(bǔ)序列或其一部分序列。
7.位于基片上的如權(quán)利要求6的診斷框架，其中所述的基片適合鑒定包含于其上的基因差異表達(dá)。
8.權(quán)利要求7的診斷框架，其中所述的基片用于微陣列。
9.權(quán)利要求8的診斷框架，其中所述的微陣列是cDNA微陣列。
10.權(quán)利要求8的診斷框架，其中所述的微陣列是寡核苷酸微陣列。
11.一種診斷框架，包括選自Seq.ID.No.42-49的基因組合中分離的核酸序列、其互補(bǔ)序列或其一部分序列。
12.一種用于診斷結(jié)腸直腸癌的試劑盒，包括選自Seq.ID.No.42-49的基因組合中分離的核酸序列、其互補(bǔ)序列或其一部分序列。
13.權(quán)利要求12的試劑盒，進(jìn)一步包括進(jìn)行微陣列分析的試劑。
14.權(quán)利要求12的試劑盒，進(jìn)一步包括媒介物，所述的核酸序列、其互補(bǔ)序列或其部分序列通過該媒介物進(jìn)行檢測。
15.一種評(píng)估結(jié)腸直腸癌療效的方法，包括選自Seq.ID.No.42-49的基因相結(jié)合，鑒定每個(gè)基因(相對(duì)于正常種群的相同基因的表達(dá))的差異調(diào)節(jié)。
16.權(quán)利要求15的方法，其中對(duì)療效的評(píng)估包括確定患者是否好轉(zhuǎn)，非好轉(zhuǎn)，復(fù)發(fā)，可能好轉(zhuǎn)或可能復(fù)發(fā)。
17.評(píng)估結(jié)腸直腸癌狀況的制品，包括選自Seq.ID.No.42-49的基因組合中分離的核酸序列、其互補(bǔ)序列或其一部分序列。
18.評(píng)估結(jié)腸直腸癌狀況的制品，包括選自Seq.ID.No.42-49的基因組合中分離的核酸序列、其互補(bǔ)序列或其一部分序列的表示物。
全文摘要
通過分析一組基因的表達(dá)對(duì)相信患有結(jié)腸直腸癌的患者的結(jié)腸直腸癌存在與否或其可能狀態(tài)進(jìn)行評(píng)估的方法。本發(fā)明還涉及以多種媒介物，如微陣列作為含有基因表達(dá)分布圖的試劑盒。
文檔編號(hào)G01N33/574GK1495273SQ0313120
公開日2004年5月12日 申請(qǐng)日期2003年3月29日 優(yōu)先權(quán)日2002年3月29日
發(fā)明者Y·王, Y 王 申請(qǐng)人:奧索臨床診斷有限公司