phosphatase-like gene in soybean induced by NaCl stress but not phosphorus deficiency[J].Gene,2003,318:103-111.
[0146] [19]Lung S C，Lim B L.Assimilation of phytate-phosphorus by the extracellular phytase activity of tobacco(Nicotiana tabacum)is affected by the availability of soluble phytate[J].Plant Soil,2006,279:187-199.
[0147] [20]Ma X F,ffright E,Ge Y X,Bell J,Xi Y J,Bouton J H,ffang Z Y. Improving phosphorus acquisition of white clover(Trifolium repens L.)by transgenic expression of plant-derived phytase and acid phosphatase genes[J] .Plant Sci.,2009,176:479-488.
[0148] [21]Madsen C K，Dionisio G，Holme I B，Holm P B，Brinch_Pedersen H.High mature grain phytase activity in the Triticeae has evolved by duplication followed by neofunctionalization of the purple acid phosphatase phytase (PAPhy)gene[J].J.Exp.Bot.，2013，64(11):3111-3123.
[0149] [22]Maougal R T，Bargaz A，Sahel C，Amenc L，Djekoun A，Plassard C，Drevon J J.Localization of the Bacillus subtilis beta-propeller phytase transcripts in nodulated roots of Phaseolus vulgaris supplied with phytate[J].Planta,2014 [Epub ahead of print，D0I 10.1007/s00425-013-2023-9]·
[0150] [23]Richardson A E，Hadobas P A，Hayes J E.Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate[J].Plant J.,2001,25(6):641-649.
[0151] [24]Robinson W D，Park J，Tran H T，Del Vecchio H A，Ying S，Zins J L， Patel K，McKnight T D,Plaxton ff C. The secreted purple acid phosphatase isozymes AtPAP12 and AtPAP26 play a pivotal role in extracellular phosphatescavenging by Arabidopsis thaliana[J].J.Exp.Bot.,2012,63(18):6531-6542.
[0152] [25]Rose T J，Impa S M，Rose Μ T，Pariasca-Tanaka J，Mori A，Heuer S， Johnson-Beebout S E,ffissuwa M.Enhancing phosphorus and zinc acquisition efficiency in rice: a critical review of root traits and their potential utility in rice breeding[J].Ann.Bot.,2013,112:331-345.
[0153] [26]Schenk G,Ge Y B,Carrington L E,ffynne C J,Searle I R,Carroll B J, Hamilton S，de Jersey J.Binuclear metal centers in plant purple acid phosphatases:Fe-Mn in sweet potato and Fe-Zn in soybean[J] .Arch.Biochem.Biophys.，1999，370:183-189.
[0154] [ 27 ] Sun F，L iang C，Whe 1 an J，Yang J，Zhang P，Lim B L · G1 oba 1 transcriptome analysis of AtPAP2-〇ver expressing Arabidopsis thaliana with elevated ATP[J].BMC Genomics,2013,14:752.
[0155] [28]Teng W，Deng Y，Chen X P，Xu X F，Chen R Y，Lv Y，Zhao Y Y，Zhao X Q，He X, Li B, Tong Y P, Zhang F S, Li Z S. Characterization of root response to phosphorus supply from morphology to gene analysis in field-grown wheat[J] ?J.Exp.Bot.，2013，64(5):1403-1411.
[0156] [29]Tran H T,Qian ff Q,Hurley B A,She Y M,ffang D ff,Plaxton ff C.Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana[J].Plant Cell Environ.,2010,33(11):1789-1803.
[0157] [30]Wang J，Sun J H，Miao J，Guo J K，Shi Z L，He M Q，Chen Y，Zhao X Q，Li B，Han F P，Tong Y P，Li Z S.A phosphate starvation response regulator Ta-PHRl is involved in phosphate signaling and increases grain yield in wheat[J] .Ann.Bot.，2013，111:1139-1153.
[0158] [31]ffang L S,Li Z,Qian ff Q,Guo ff L,Gao X,Huang L L,ffang H,Zhu H F,ffu J ff,ffang D ff,Liu D. The Arabidopsis purple acid phosphatase AtPAPIO is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation[J].Plant Physiol.,2011,157:1283-1299.
[0159] [32]ffang X R,ffang Y X,Tian J,Lim B L,Yan X L,Liao H.Overexpressing AtPAP15 enhances phosphorus efficiency in soybean[J].Plant Physiol.,2009,151: 233-240.
[0160] [33]Zamani K,Lohrasebi T,Sabet M S,Malboobi M A,Mousavi A.Expression pattern and subcellular localization of Arabidopsis purple acid phosphatase AtPAP9[J].Gene Expr.Patterns.,2014,14(1):9-18.
【主權(quán)項(xiàng)】
1. 大豆紫色酸性磷酸酶GmPAP36，其特征在于，其氨基酸序列如SEQIDN0:l所示，或該 序列經(jīng)替換、缺失或添加一個或幾個氨基酸形成的具有同等功能的氨基酸序列。2. 權(quán)利要求1所述大豆紫色酸性磷酸酶GmPAP36的編碼基因。3. 如權(quán)利要求2所述的基因，其特征在于，其為大豆紫色酸性磷酸酶GmPAP36基因，該基 因的cDNA序列如SEQ ID N0:2所示。4. 含有權(quán)利要求2或3所述基因的載體，其出發(fā)載體包括pCamE、ρΖΥΙΟ 1。5. 含有權(quán)利要求2或3所述基因或權(quán)利要求4所述載體的宿主細(xì)胞或工程菌。6. 權(quán)利要求2或3所述基因在提高轉(zhuǎn)基因植物對環(huán)境中磷元素利用率中的應(yīng)用。7. 根據(jù)權(quán)利要求6所述的應(yīng)用，其特征在于，所述磷元素以有機(jī)態(tài)磷形式存在，優(yōu)選地， 所述有機(jī)態(tài)磷為植酸磷。8. 根據(jù)權(quán)利要求6或7所述的應(yīng)用，其特征在于，所述植物包括擬南芥、大豆。9. 權(quán)利要求2或3所述基因在植物品種改良中的應(yīng)用，其中所述植物包括擬南芥、大豆。10. -種轉(zhuǎn)基因植株的構(gòu)建方法，其特征在于，采用農(nóng)桿菌介導(dǎo)法或花粉管通道法，將 權(quán)利要求4所述的載體轉(zhuǎn)入植物中，篩選轉(zhuǎn)基因植株。
【專利摘要】本發(fā)明提供大豆紫色酸性磷酸酶GmPAP36、其編碼基因及應(yīng)用。本發(fā)明基于抑制性差減雜交技術(shù)構(gòu)建低磷誘導(dǎo)大豆根系cDNA差減文庫，從中選擇低磷脅迫誘導(dǎo)表達(dá)的酸性磷酸酶候選EST，在此基礎(chǔ)上，克隆酸性磷酸酶候選基因序列，采用實(shí)時定量PCR技術(shù)分析候選基因在植酸磷處理?xiàng)l件下的表達(dá)差異；構(gòu)建基因原核表達(dá)載體，實(shí)現(xiàn)基因原核表達(dá)，同時構(gòu)建基因真核表達(dá)載體，并轉(zhuǎn)化擬南芥、大豆，獲得轉(zhuǎn)基因陽性植株，分析基因GmPAP36的生物學(xué)功能，為植物磷高效轉(zhuǎn)基因育種提供功能基因。
【IPC分類】C12N15/55, C12N9/16, C12N15/82, A01H5/00
【公開號】CN105647884
【申請?zhí)枴?br>【發(fā)明人】張彩英, 李喜煥, 孫星, 段鵬博, 孔佑賓, 杜匯, 李文龍
【申請人】河北農(nóng)業(yè)大學(xué)
【公開日】2016年6月8日
【申請日】2016年2月2日