專利名稱：人類肝臟相關基因的制作方法
技術領域：
本發(fā)明涉及生物技術領域，尤其涉及一類在人類肝臟中表達的相關基因。
背景技術：
肝臟是人體的第一大器官，是物質代謝、能量轉換及供應的“樞紐”，是機體內多種重要信息調控分子的“集散地”，是機體內再生能力最強的器官，在人類生命活動中占有重要地位。肝臟的主要功能是進行糖的分解、貯存糖原；參與蛋白質、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防御機能；制造凝血因子；調節(jié)血容量及水電解質平衡；產生熱量等。在胚胎時期肝臟還有造血功能。
肝臟是淋巴細胞以外最常見的病原體持續(xù)感染的場所，肝臟疾病又分為肝炎、肝硬化、脂肪肝、肝癌等?，F(xiàn)代醫(yī)學實驗證明，肝病病毒侵入人體后，并不直接引起肝細胞的損害，只是在肝細胞內吸收營養(yǎng)賴以生存，并在肝細胞內復制、繁殖。其復制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細胞膜上，引起人體免疫系統(tǒng)對這些抗原物質產生免疫反應，這種反應造成肝細胞的損傷、壞死。免疫反應的強弱決定于肝臟受損程度及臨床癥狀輕重。這場由病毒引發(fā)的、免疫系統(tǒng)對肝細胞的戰(zhàn)爭，使大約25％的患者的肝臟成為戰(zhàn)火連綿的戰(zhàn)場，肝臟的損傷由此加重。肝病的危害絕不僅僅限于肝臟本身，它還可以引起其它多種疾病。常見的有(1)糖尿??；(2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎病；(6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結節(jié)性動脈炎；(12)消化性潰瘍；(13)自發(fā)性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對患者的身體甚至生命造成危害，而且對患者心理上的打擊也是十分沉重的。無論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學等方面都會受到嚴重影響。
肝臟疾病相關基因在以肝炎、肝癌為代表的重大肝病的控制、診斷、防治與新藥研制領域起著重要的作用。
在本發(fā)明之前，還沒有出現(xiàn)涉及本發(fā)明的一類人類肝臟相關基因的公開報道。

發(fā)明內容
本發(fā)明要解決的技術問題是提供一類在人類肝臟中表達的相關基因。
為了解決上述技術問題，本發(fā)明通過如下技術方案實現(xiàn)在本發(fā)明的一個方面，提供了一類在人類肝臟中表達的相關基因，其包括(a)SEQ ID No.1～SEQ ID No.40所示的序列；(b)SEQ ID No.1～SEQ ID No.40所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.40所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.40所示的序列。
本發(fā)明還提供了一種探針分子，所述的探針分子含有上述序列中約8-100個連續(xù)的核苷酸。
由本發(fā)明的一類在人類肝臟中表達的相關基因，可為研究肝臟疾病的致病機理以及開發(fā)治療肝臟疾病的藥物發(fā)揮重要作用。
具體實施例方式
下面結合具體實施例，進一步闡述本發(fā)明。應理解，這些實施例僅用于說明本發(fā)明而不是限制本發(fā)明的范圍。下列實施例中未注明具體條件的實驗方法，通常按照常規(guī)條件如Sambrook等人，分子克隆實驗室手冊(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來源于5個成年男性，在肝臟切除手術后，將肝臟組織立即置于液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩后，再移入玻璃勻漿器內，勻漿后移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑒定總RNA質量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實施例2cDNA文庫的構建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補平末端后，加含EcoRI切點的接頭。磷酸化EcoRI末端后，用XhoI限制性內切酶消化1.5小時，再進行片斷分離。過柱篩選長度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，無菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進行包裝，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)細菌。涂板并測定滴度。
實施例3測序及數(shù)據(jù)庫建立(Seqencing and Database Constructing)挑選文庫中有外源片段插入的克隆，擴增后抽提質粒(Qiagen Germany)，用T3和T7作為3’和5’端的通用引物，采用終止物熒光標記(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377測序儀(Perkin-Elmer，USA)上進行EST大規(guī)模測序。測序結果用FACTURA軟件去除載體序列，傳輸?shù)絊UN Ultra 450 Server上進行下一步的處理。所有的序列信息再用GCG軟件包(Wisconsin group，USA)中的BLAST和FASTA軟件搜索已有的數(shù)據(jù)庫(Genebank+EMBL)，將無同源性或同源性低于95％的序列視為新基因建立數(shù)據(jù)庫。
實施例4基因的全長克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎上，進行cDNA全長克隆，分兩階段進行(1)“電子克隆”(Electronic Cloning)以新基因片段序列作為探針搜尋dbEST數(shù)據(jù)庫，將重疊序列＞50bp，同源性在98％以上的表達序列標簽(Expressed Sequence Tag，簡稱“EST”)序列認為同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER軟件進行連接，部分EST可以延伸探針序列。再用STRIDER軟件分析被延伸的序列是否具有完整的開放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和氨基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長完整性如何。通過電子克隆的方法，通?？色@取人肝臟相關基因的全長序列。
(2)cDNA末端快速擴增(Rapid Amplification of cDNA Ends，RACE)如果通過“電子克隆”方法仍未得到完整的cDNA全長，則在已有序列5’或3’端設計引物，在人類肝臟Marathon-Ready cDNA文庫(Clontech Lab，Inc，USA)中進行長距離PCR反應。然后對PCR產物克隆、測序。用AUTOASSEMBLER及STRIDER軟件分析被延長的序列有無完整的ORF，如無，重復上述過程直至獲得全長。
(3)RT-PCR對于5’和3’端的已知的序列，如果中間有一段間隙(gap)無法從已有的公共數(shù)據(jù)庫或自身數(shù)據(jù)庫獲得，可考慮采用RT-PCR的方法。在序列5’端設計引物，3’端引物采用Oligo-dT，在肝臟總RNA庫中進行擴增。然后對產物進行克隆、測序。最后拼接便獲得全長。
通過組合使用上述3種方法，可獲得人肝臟相關蛋白的全長編碼序列。
序列表<110>上海人類基因組研究中心<120>人類肝臟相關基因<130>NP-10159<160>40<210>1<211>969<212>DNA<213>Homo sapiens<400>11 cttactttat ttgcatctca ncaaaggttc tcatgtggaa cctgattggc atcaaaccaa61 agttcgtagg ccaacaaaga tgggccactc acaagcttcc catttgtaga tctcagtgcc121 tatgagtatc tgacacctgt tcctctcttc agtctcttag ggaggcttaa atctgtctca181 ggtgtgctaa gagtgccagc ccaaggtggt caaaagtcca caaaactgca gtctttgctg241 ggatagtaag ccaagcagtg cctggacagc agagttcttt tcttgggcaa cagataacca301 gacaggactc taatcgtgct cttattcaac attcttctgt ctctgcctag actggaataa361 aaagccaatc tctctcgtgg cacagggaag gagatacaag ctcgtttaca tgtgatagat421 ctaacaaagg catctaccga agtctggtct ggatagacgg cacagggagc tcttaggtca481 gcgctgctgg ttggaggaca ttcctgagtc cagctttgca gcctttgtgc aacagtactt541 tcccaggatc cacaggaaat actcagagtc cacctggaca ttttacttat attcagtttc601 caagtgtcag aggggttcag gactgtctgg cctggggggc ctcctcatga cctcaattca661 ggattatcta ctcaaagcat taaacaaaag gaaatacatt ttcaggatga aggctgctct721 tttaagccct gctgaagarg catttcaaac aggattggaa tagggaaacc ggcactcagc781 tcggcgcaag ggcgggtgcc ttcagactag agagcctctc ctccggtgcg ctgcaagtag841 ggcctcggct cgaggtcaac attctagttg tccagcgctc cctctccggc acctcggtga901 ggctagttga cccgacaggc gcggatcatg agcagctgca ggagaatgaa gagcggggac961 gtaatgagg<210>2<211>1162<212>DNA<213>Homo sapiens<400>21 ggnsggctgt gtggtctggt gtttgggagg gaactccacc cccaccaggc caaccatgga61 gctagaaaca gagacagcag ggagggcaaa gctggccact gcctgctcca ccccttcaca121 gcccagagca gaacagggtc tgctctactc tcaaggtgag tgacagagag ccggtactgt
181 ttctgcccct ggcataccct gagaacccat gtgacttctg ttgtgctcag cccctgtgcc241 cttcctgggc ctgatccaca tgtgtcaaca cacacactcc ctctcacagt ctccaaacag301 cactgcagag cctagctgca tctgccaggt tcaaagagga atttttcaca tttgctcact361 tccaatctcc atcttccttc ctctgtctcc cactctccca ctctcagtag ccgcatccca421 gccctgccat actcccttct cagggacagg agactcagtg ggcagctggc ctcagctctc481 ctaacaggaa aaaaacctgt acagcattag tgccagggct cctgccctcc caagcgctga541 gcccagaaat ttggacaaat gagctgcctc ttaactgcaa aaaacaattt taaaaaagca601 aaagatcaaa caaacagacc aaaaagcata aataaacagc agctgggcca gcgaggagga661 aggcagggtg accctcagtg gctccctgtg cccatctcag cctcttgcca taaaactcag721 ccatcagtgg ccaggatgac agcagttccg aagatgccca cactctctcc aaggagcttc781 atctggttcc agaactcaac acgccgcgtg ttgtgccagt kagcaacatt gccatcaatg841 agcagcatga cagggggcag cagtwcagcc aggatctggg cagcgagggt cacgtagtag901 ccttgacaga aaggccaggg ccaggatgcc ataaagcacg gagaacagct ggatcatcag961 ctccctcctt gggagatggk tcagatacgc cagcgggtct ccttgctgtc tgcagtgagt1021 gggcaaacag atgaggtaga tacccaggaa cactgggcgg gggrctgcag gagsggctgc1081 agggttycgg cggtamgctc ccagcaccgc gggcaacaaa gaaagcgcgg atgwkgsaat1141 gttcgcrrtt mataggactt ag<210>3<211>1125<212>DNA<213>Homo sapiens<400>31 ccatgtttaa aattttgaag aaatccctgg agtagacaat ccctagagca ctgacatgct61 gtggtccaag gttcaccagg aacagtgcct gtgccctgcc acatggcggg gttccttcaa121 gcctctggtg atgaaggtgc tcagggaaag gaggacggag gggcgcggaa ggacccagcc181 actgctggcc accctggcct ctgctctgtg ttcacggggg agcagactct ggggaggcgc241 ccctcctcac cacggcctgc aggcgggctg gagcctgcgc cttcaggggc cttgcatctc301 cagctggacc ccctggcaca gatgtgctgc agtagcccgt tgaccacatc cagagtctcg361 tccttctcca gcacgatgtc ataggagttc atgtagcgct cccgccgctc ctccaccttg421 tcattcagga agccaatttt gagaatgttc tgcacaccag gaaccccatc ggccatggtg481 aggtccccga tagagtctcc cagcaggatg acattggttt tgccctcaag ttgctggaag541 taaccacagt tctcacacac agagctgttc ttgttgtatg tgtgtatcag ctggccctta601 aatccctgga gaaaaccctg agcattgcat tggactctct aaccacctgg gctatctgaa661 acttctgaat cttctgctga cataggagat tgtgcgcttt ggtccaccat tccaccatat721 gaggtagctt ctccttgacg gtccggtgtg ggtcgatctc aattgggtaa tagtggtgaa781 ggagcgctgt gagctctttc cgacactyct cactgatgat cttgctatta tccagaatat841 tgtwagaaga agggcatcgc tttccattat atgcaaacct gctcaaggtc atgtcaaaat901 cagaaatcac ctgttaccgg tctccgccgc ccttgcggag ggcgcccacg atctcctgcm961 ccgcccaggc tgccgcatca ggaccgtggc yttcatcagg gtgctcactt tsccgagacc1021 gagaraacam tkaccctgcs camgctgggc csgaggggrc cgggggycgy cggggcscgt1081 ttaagggggg cggggkggca ggggsgctmt gcctggtgaa sgcgg
<210>4<211>842<212>DNA<213>Homo sapiens<400>41 tttaatatca taaatcsgtg tggggccaca cctctcactg ccaggcccgt gggctcctat61 ctccatattg cctggsagst gcctgcsgcc aggscttccc ccatccccta ggaaggatcc121 agcaccagcc tgagavggga ggcaacagca gccaaagggg tgggaggvgg aggagacaga181 agcatgtscb gcaggcgcat gtgtgtttgt tcttacatgt gtgaacacat ataaagtgtc241 aggtttacag accctggctc aaggacagty taggatggga aaggaggtag ggcgagaaga301 agcacatatt ttctccctgg tgcttcagcc tcaccctatc caagggacag acatatgggg361 tgtgagamac ccatccccag gtcccagcct tcaggactgg agtccttttg agtctggtgg421 agtcacagac ccagtctttg ggggacactg ggtctgtctc cttttgaaag ccctggaaag481 gygggaggta agaagtaaag ggagaaaggc ccctgctaga agaacttgac gcctcggcca541 tcgctgacag taatgatctc ggccttgtgc tcctgctgtc gggcctgcag agcccgcagt601 agagtggctt catccagccc gtggaactcc tcaccctctg tgtctccccc attagtcagt661 ccatacaggg taaagacgga gtggttctgg ccactcctgg aaacccactg atagatgagt721 ttcccccatt cttctggcct ccgccacatg atcaggaagc tggacttgct cttatccaac781 cactcgaggt tccctttctt cctcagttcc tctaatacaa tctggatcga ctccacagga841 ag<210>5<211>854<212>DNA<213>Homo sapiens<400>51 cgcacaccga gagccacttc cggaacaagc gtcgcgtntt ctgaggagaa actcttggtg61 agaattccca gagtgataat ggctacctac agcctggcga acgagagact acgcgctctg121 gaagacattg aacgggaaat cggcgccatc cttcagaatg caggtactgt gatcctagaa181 ttgtccaagg aaaaaactaa cgagcggctc ctagaccggc aggcggcggc cttcaccgct241 tcagtgcaac acgtggaggc ggagctgtca gctcagatcc gctacctcac ccaggtggcc301 acagggcagc cccatgaggg ctccagctac tcttcgagga aggactgtca gatggctctg361 aagcgagtgg actatgcccg cctcaagctc agtgatgtgg ctcgaacctg tgagcagatg421 ctggagaact aggccaggga gatggaccca gagctgagag ggagaccatc acctgtgcca481 tgggacagaa cctgggaaca tgtagggtgg ggagtatgag caccaacata ccctgctggt541 caaagtaccc taggacaaag gggcaaatgg tgggcatgga aaaactgaag cccgagcctg601 cccacagcta tgccttggcc tctccagcag ggtgctcctg ctctgcactc cagaaacacc661 ctgacaggct cagagaggag atggaaactg acatacccaa cccacttcct caaacacttc721 atgctgctgc cccagccttc ccgcagactt ggcctaatga aaatacatac ttcttcattc781 ggagagacaa aacaagaact agagttttaa tgataataaa agcaataata attaaagcat841 taccataaaa aaaa
<210>6<211>798<212>DNA<213>Homo sapiens<400>61 tataatggta actgtaaaat gtactatttt taatgggtgt gcatgtcaag mttttcttta61 gaaatacact ggtctggtct aatttattta agcaggagca ctttaaagta tcccacccta121 ccccattcca cccccagtgg acagaaagga aattgactga cttgagrgga tgcagacatc181 tgggttattc caacagacca gtggttagga ggagggggtg ggtagcatta tggcctcggg241 caggcccccc caccctgagc ctctgaaagc tgactttatc tgtaagaggg aggtcaggct301 cgccttctca atancgtgta tttggataar atnagtttct tctggantac acttaggcan361 ancanggctn gcttanggct nnancctcag cgtttcacaa cttctcacct tctcttccat421 gtcagtcana atggcatcca actttgccac cacctnccgt gcattgtcca nnctnaanca481 cattgggggc ttaaacttca agatnttcct cccagggcca tcagtgctca gcaaaacgta541 ttctccttca gccttgatac caagtaggca gcctcttcag ttgctggtgt ccttgtggcc601 tcatctttga tcagatccac accaatgaag agcccaacac ccckgacatc cccgacgatg661 ggatgtttga ttttttgctg cccgaggagc tgcatcagga agctgcctac actggtggca721 tgatcctgga gctgctcctt ctccaagaca ttcaggacgg ccatccccac agcgcaggac781 actgggctgc ccccaaac<210>7<211>1162<212>DNA<213>Homo sapiens<400>71 aatataagca tttaatgtca aagaaataaa ggtaatttta caaactcagt ttttgtaagt61 acatgaagtt tctatttgat tatgtggttt tatatcacat tcgttcaaat gcatttctct121 cccttagagg gactattcca acatcaatmc tttggaatta tttcagtcat ccttaacatg181 tgactttacc aaagaccttg aagctaaaca aacaagcaaa acaaaatttc aatgaatctt241 agatgaatgg aataagaaat agtmatcaca tgtaaattag ggatgttcat ctccaaccaa301 gaaactgtca aaatgtttct tctgatacag cagttataag tcagagcctt caaaaaamaa361 gggcagaaca agagtacaat aaaagaagca tctgcaactt aagcmtccca cagtcctaag421 cctgatatgc gcaaagaaaa gcctctttcc cgcaaactaa attccatcca tttgagcttt481 cagagatcga tgcccaagag ctatcattaa tatatacagc atattgactc tagttgcatc541 aattatgcct gaagagttta atacccatcc aagtccaaag gtggaagaat acatacactg601 gtatggtaat aggcaagagc aggctgtaaa agcaaaggct ggctgtgcta gtgcagccct661 gtgggaagtt ttcttccaca gaggctgagt agaacagtcc tgctaaagaa accagtggaa721 taagaacagt caacgtagga agagacagaa acattcttct cttccactta ttacctgcca781 ctgtgtttta aaaggtatat ggtattaaga aaagttggct gttgcgtttt tttaattttt841 ttaaattatg aactagcgct gctccacttc ttcttgcttc ttactttgac gtgtcatcct901 agctgcctgt ggtatcatat tagggatccc atcaatgatt ggataagcta ttccaactct961 tcattaatca attcgtttgt tgatgcttca tatctgagcg gcttcttkga ggagcgggca
1021 cacaaggaac tccagcagcg csggrtcgaa gtcgcggggg ggttctcagt yttyttgccc1081 gggcggccaa aggsggaccc saagcgtgsa ggsactamgg gggacgsggr gggggggggg1141 gggcccgcag gstgggggga gc<210>8<211>908<212>DNA<213>Homo sapiens<400>81 ggtgtttcat ttntngtagc ccagnnatgg nagtgcaatg gtgcgatcwt agctcactgc61 aacctccacc tcccaggttc aagcagttct tctgcttcms cctcctgagt ygctgggatt121 acagatgtyc gcsaccatgc ccagctaatt ttgtttgtat ttttagtaga gacggggktt181 catcgtgttg gscagggtgg tctcaaactc ctaacctcag gkgatctgct cacctcgggc241 tcccaaagkg ctgggattat tatgggcata agccactgtg cccagcccaa aaaggsattt301 taaacaaaaa ttctgacgtt taaaaactga aaatatatcg cgccactgca ctactgcctg361 ggcaacagag caagamtctg tctcaaaaaa acaaaacaaa aaacaaaaaa ccctgaaaat421 atgagcccag gagttcgagg ctgcaatgag ccaatactgc acctttgtgc ttctgcctgg481 rcgacagagt gagaccttgt cgataagaaa aaaaaaaacc tgaaaacaga aaagagggct541 tttacaatgt tgatgaactc atcttagagt gcatgattta taaatacata aatatccatg601 catactttta cagtgataac tcctgggaaa atgagaatgt taaaaaagcc aatctagaca661 gagtccttct cattccacct gcctctttat gttggtaata ccaggagtct taagagagct721 gctgtggtct atgtcacatt catcccagcc tcctttctaa tcctgtagct gatgatgaaa781 atgtgataaa tcttctggct tagctttgat gctttttcat taattcactt ctggtagtaa841 attgtggcag gyatyaaatt maatcamatm ggcaatttma ataamattgt ataaaagsya901 tttattgt<210>9<211>1070<212>DNA<213>Homo sapiens<400>91 attaatctat ttaatttttt tttttcagtt tgtatttttt attttacaat aaaaagtcaa61 aaaaaataag caaaaagata aactggaaaa taagayttac atctcatata tatggacaaa121 ggaccaatta cctccaaaca taaacagctc ctagaaatta ctgcaaagat caacaaccca181 gtagaaaaat gaatgaagtt cccagaacag aaaacacaag tggcccttca aaaaaatgaa241 gagaggctca gcctcttatg gtaagacaaa gagacaggat tttaaaaacc taggcctctt301 cctagagttc ccttaaatat ctaggccaga tcatttttac ttcctggctt agaccctgcc361 aagggctgcc cagctactca ggtgtttgtg tacttgtgga ctcaagtyat attgtcctga421 tcttctggct gagtacggtt ttctcctcca gcaaagacaa tttggaggat gtactaagca481 tgaagcgcta cttcctggcc cccatctctc ttcgcacagt gttccatcat ccagccatga541 aagcacagct gagtgatcca agaggcagtt ccaattgttg actaacgtgt acctgcctat601 gtgagtgtgt tctatgggaa ctcaggcctt agaatggttt caaagtagtg gctttcaaaa
661 ttactgtttg cctcttcaaa cttcacacct aaggaaaatg gaaacatgca gagcagggac721 acagaagggg cattagctgg cgtggggtag gggcaagagc taattgtgaa ggaagaaggc781 ctgagatcac gtagccatgt cggagaacag ctgtgctcgc tgccctgcct ctttgcgcgc841 atgttaggca gccccaggct ccagctgctt gagtttctct tggagtgccc ggagctggct901 tcgaccccag ttaatgcggt tctggaaaaa aaaaawatwt rcggscagct gcaggscatc961 ccggaagctt gcttgagctt gaggtgactg ttaggactwg ggttccaacc mgttcagggg1021 gtctgagggg ctaaaagrct csgktctggg gttaatgggc cytkccctcc<210>10<211>978<212>DNA<213>Homo sapiens<400>101 tgctacttgt ctgagtnaaa ggccgnnaca ttcggttgac tggggaagtc cttctgcatt61 aattacaata agtaatactc attgaaaact ttccttattt acacacagtt gaacagttat121 tctaagaaac cattttccca ccctatttaa tattgacaga tttcttcagt tatnnacaaa181 aactcnnnac ncncgctwtc ctwaakwact cwtctwtkty ccccttatyt cccctcatct241 twtkttgtyc tctwwataca gggyttgctg ccctttcttc ttcttgcctt ttyagcagwt301 gtkttaaggt tgcagaggtg gcagttaacc atagagtyac agaktttgat ttttttccat361 ccwtcaggtc gtagaagytg ccactgagcc tkwttaagak ttggtactcw aaaatgcagw421 ttkaattagt ttgaatgagg tgwttaaggw ttawtgaktw ktggttwagg aaacaaagwc481 cattwgtggt tggggtwatw ttgctgtttt actgttggaa gcwggtttct ctaawwwaac541 tttctgctgc cwtttacttw catacatttt aaagagttac tacaagattt tttaaaaatt601 taaacagtta ttacaagtta atatatgtag tgtattgttc atgacactaa actgaactgt661 tcatgacaca aagtatctta gaaaacaaat ctcggctggg ccagcacttt gtgaggtcaa721 ggccagcaga tgacttgagg tcaggatttt gagaccagcc tggccaacat ggagaaacca781 catctctact aaaaatacaa aaaaattagc caggcatggt ggckggkgcc tgthatccca841 gctactgggg aggctgaggc aggagaatcg cttgaaccca ggaagtggtg gttgcagtwa901 ggtgagatca taccactgca ctccagcctg ggcaacagag caatattwca tctcaaaaac961 aaaaacaaaa accctagg<210>11<211>571<212>DNA<213>Homo sapiens<400>111 gccttgtgat tcaccttact ctcggccatt tcctgagcca cgtctgaaga aatcctgtac61 tgccaagata tatcctcaga caaagaaatg tccattagtc actcactaaa agggtaaaaa121 gcaatcgaaa tgttttccta tctgaacccc tggtgccagc acagtataca gtagacaata181 tatgccataa tttattgcct gaactcccca ttgatgtgac catgatatgt caatggaaag241 tgagttcata aaaaataaat tctcattctc ttcaaataaa tttattgaga ttctatgaca301 ctcccagaac gtggatatag caaacaacac tatgtaccat ccatgaaaaa acttacaatc
361 cagtaggaga aaaagacaag taaacaagca gttattgtag agcataataa gaaataaatg421 aaaactgcca tatgggcatt ttagttggta cagtcaatgc caaatagaga gagcagtanc481 agtttcctga aaaagtgatg gctanggtga gacctaaatg ccggtaggaa ttnaactggg541 gnaaatgttg gaagcngcnc actttangga c<210>12<211>639<212>DNA<213>Homo sapiens<400>121 atttgtagcc tgtggcaacc gtacccatct gtatccccaa gttctacacg gaatccctcc61 agcacttcac gactccatta tccagctacc actaattcct ttgtgggcag agtccagcca121 cgggcagccc ctgccctgcc tctggtcagg acaacacctg cccaaggaga gcaagggcct181 gtgacctctg ctggcctgtg cgccctcacc tgtcacccag ctcagccctg tgtctggcct241 acccagggag gaaggaacca gatttatcag cctgaaagga ggtagaagtt ttggagaaaa301 gacactgttc gggtgtcaag taatgaactg actttattcg aacctcatct ccaatccaat361 cccaccgtgg tctctctctc cctcagcttt tctggggcgg gttgactcgt gtgtcacttt421 atcacagggg cacatggggt gagaagagga accttggcag taggagatac ctttgtacca481 ggttntaatt tccagtgtac atctcagtcc cctcccctaa ccctcaacac agcctgggga541 aagcccagct tgcaacacca gttctcagcc cagactgaca gccagataga tcttggccct601 gagttnngcc aataaagatg ccgtgattat tacctaana<210>13<211>652<212>DNA<213>Homo sapiens<400>131 caggcccacg ccaccgtgcc tggctggaca tgtaaatgtg aagtgaatgg ttaaacatgc61 agctagctga aagcatggca gaccctaaca gaaaagctac agtgtgtttt tgcagctatg121 aagtgaatgg tttcctgggg aaaattgtga ctttgtataa ctgttgttga aaccagaata181 aattatattt cacttgcata tgcttaaatt attaaaattt tcagaagtca gtgatacaga241 agtactattt tgcaatgtta atctgtttga gtctttggag aaagtggttt cattataggt301 acatagtgca ctgttaatat tttaaacaaa tagttcactc ttccatttaa gggatagcag361 ttccttgtat aaaatgactg gatgtgtata aaggaattat gttgtcatgt gcctttaacc421 agctttagta attactataa tctcatattt atgatagctt tgttaggtga caggaccaaa481 tgacaatatc ttatgctttc tcatcacttt agaatttatc attatgtaca ttactggggt541 tttagcattt cctaatgtga agttttaatc actcttaagt atacattctt ttctggatca601 cttaaattaa atatttttat aacctcttaa aaaaaaaaaa aaattaactg gg<210>14<211>292<212>DNA
<213>Homo sapiens<400>141 cgtcgaccga gaagacgaca gaagggcaaa atgatgagat tccacttaat tggtccgtgt61 ttctgaaaca catgatattt gtggaaattc tgagcaaact atagaacaca ccccagggct121 aatttgagat aagtacaatt aagttaatct acatatatct aaaaggtttt ttttgtaata181 agtatctgat ttccggaaga tttactttac tcaaggaact ttaaaggttg tatcccccac241 caaaaaaaag aaaaaaaatc agaaggccaa ctgcgcaaac aaaaaaaaaa aa<210>15<211>522<212>DNA<213>Homo sapiens<400>151 tttttttttt ttttttcctt ccttatttta cagctttatt actgatttcc ctctaaaaag61 aaactcaagt gtggagctga ctcatctata gattaaggaa tcacaaagta ccatagtcac121 ttaacaaatg caaaaaaaaa aaaaagcaat tgttttacct ttcaactgct gatgttatgt181 acaatctatc acaaaaagca tagctgggca ttcatgagct gattatatca accctctaga241 attttaaaaa cgagcccatc taagcatatg gatactctct taccttacaa agtaaaagca301 ttttctattt taaaattttt atttctgggt aaaacaaaga attcctagag gtcaaataat361 ataccaaact taaagcacta ttaagtcatg aaacaaagaa gattctgagg tagaagtcaa421 gcaatataat ccacagtttc aggtattccc agcaaccatc gcatttcgca agtaagatct481 atttttttta aagtctcaac ttccaaaaaa tcaataatcc at<210>16<211>475<212>DNA<213>Homo sapiens<400>161 atctgagaag gttctggttg agatttattt agtcaacaca cacacattaa gcatttaatc61 tgaatagtca catttccaga cacttggcat tattcaacga acaaagcagt tgaaagtttc121 tgggcctcaa tttcatttgg ggagtcagaa actcacagta gacattataa gcagtagaca181 ttatatgtca cgagaatgta acatatgatg cctttgtgtc ctcctctagg taccagatgc241 atcctcctag attctgagaa agatgaagga tctcagaacc aattataact tttaagtgaa301 tgcagaaaaa gaattttaga aaattcaaca ttactttatg acaaaatctc aaaacaaatt361 acatatagaa catacatacc tcaagccaat aaaggccata tatgacaaat ggacatctga421 catcatgcta aaccttcaaa agttgtaagc cttttttcta agagttatag taagg<210>17<211>504<212>DNA<213>Homo sapiens
<400>171 tcagttctgc acaatcctca ctttatcccc ccgaatcccc ccaccagagg gccatggagg61 gtcccactgg gcaccaggcc tggtccatgg ggagcgacaa agaaccttct gctgtcatta121 accgcgatgg tttatcacaa tgatgaccct ccccgaggcc tctcctacgg taacagcaac181 atctacggtt ccacacccac agggaaaacg gattcagagc ancagcaggg ggcgggcagg241 ctgtggggcc gacagtgaac atggaggcag gctggtggct gggagggctc catgtgggcc301 tggctgagga gccagggatc tggagtcaga accgggggaa ggcagctgga acccagtagt361 ggacttggaa ttccgcaaat cgtcctcaaa cgtcttcagc cagaagcgac ggcggcagcg421 gcgcctgcag acaggcgtca ggaagcttcg ctgctgaggg tatttctcag gctgggctgg481 gctgggaggc ccaggtcctt gcag<210>18<211>451<212>DNA<213>Homo sapiens<400>181 ttttttttgt atatatgtac agaaatttat tataaatttc attgataaaa atgatgtgca61 gcacataatt tataaataaa atatacaatg ccatttattg taattctaga aaagtttttt121 acagaatgct tttgttgatt tttgctgact cttctatcca acctgtttgc aattcaacca181 tgatatgaca caaccagact acaaataaat gtttgttact atctgattca acaaagcagt241 cactcatttc atggaatgga tatgggctgt tactcagtga aaagacacat gagatggttc301 agtgctaact cacccttctg acagaaaatg ccagaaagtt tccaccccat tgacaggctt361 ggagtcttga gtaccatctg ggtgggtggg ctggcactgg cacgaaacct ctccactgct421 atcctgacag agatggggca tgtacttccc a<210>19<211>641<212>DNA<213>Homo sapiens<400>191 gaaagatttt tttgtttatt tggtatgggg tcttctgtag ctcacacaaa attattggga61 aaataaataa cccaggggag ccccctttgc ccacggttcc taggtcgcgg agtcacagca121 gccccaaagg ggcaggaacc tgggaatccg aggggtcccg gccctggcgg tcacttgccc181 agcaggaggc cggcgcgcag cacgcggggc acgcggcgga tggtcagcga gacccgggtg241 ccgcgcacca ggcaggctcc cggccgcgcc gacgggcagg ctgccgcatt gggcggcgag301 gaggcggcgt ccagcgcgtc tacgcgggcg gcggcgatgc cgtggagaag acgcgtgtag361 gcggggccgc ggagcaccag caggctgcgc ggttccagca ntaacgaggt ggtgggccgg421 ggcggaggcc gaggctgttc tgtanggtca tcgtcctctg gccgccgcgg ctcgtagaag481 tccagcacgg tgtgggagcc caggctgatg gtgctgacag tcnggtagta cagtggtccg541 tcctcgtggg gcatgatgcc ctccacaggc agatactggt tcacgatgac attgttatgc601 tggatgcctt catagatgct gatgtttgac acttttgtca c
<210>20<211>599<212>DNA<213>Homo sapiens<400>201 tttttttttt tttttttgcc tttgacaagt tttttattgt gaaatattgg atctaaaaaa61 aagtacaaaa tgtcaacagt cagatgtgta caagtacagt atttcaatag tctatacatg121 tgaacagctt aacaggttca ctgcagaatg catatttaga ccaatataat ctaaaaagga181 ctgcaatatt cacaggctat taatacataa ataacttgag tcaaaatgac ccttactggt241 aaatgctaat taatagtgga aaacaggcta aaagcacaat acaccacacc atagtatctc301 cttacaggtc cacatttaaa gaatatcagt ttattatgtc aaaacactac agagatccag361 gactgggact gaattactaa tttatacaat tgcaaaaaaa aaaaagttat aaaataaggt421 catggaataa agaagggtat ttaattagga ctaaatactg tgaagcccat gaaagggttt481 tagccttgga atttacaaag ctaaattatt agactgttaa tgttagctaa cactaaaact541 gaattacctt ccattttaat gttagctaat attaatattc aactttttaa ttttgatgt<210>21<211>478<212>DNA<213>Homo sapiens<400>211 tttttttctg tagagacagg gtctccctgt gttgcccagg ctagtgtcaa actccaggcc61 tcaattctcc taccttgacc tctgaaagtg ctgggattac aagcatgagc caccatgccc121 cacctaaaac attttaagtg ccatcaagag aaatcacagt attttaaagc tggcttattt181 cagatgatgt ttcagaacaa agaattttcc tattttgagg caaacagaag cagaaaagaa241 aactgcatta gccaggaaga tgtgaaagac acaaagattt tataaatctg tgaaatccag301 aaaggctgaa ggaaaatttg gaattgctga aggaaaccaa aaagattata tcaataatac361 aattaatagg gtatatgaga gacatatttt taaaataaat attctctata taaaacaaag421 catgcttcaa acaaatacaa tatatttatt acacaaaaat gtcataactg acaaactg<210>22<211>547<212>DNA<213>Homo sapiens<400>221 aagagcgaca gcaaacattt attgagagct gtaaaatgtg tttcacccaa ttgtaatgta61 aagaatcgaa aattttaaaa aatttccaaa aaaattacat gattgtagtt ggtaaaaatg121 cacccatttc aggaaggccc ctgtgcctgt gtataaatga tgtgtcttga acgctgctgg181 gaagctctat tgctgagatt gagaagacct tagaaacgga aggacactct tcatcagagg241 acattaattc atttgacaag catctagaaa atgaactact tggggaaagg aaatactatt
301 tgcttaacag aaggctatat tttagcttca ttggggcgta tcacttagat ttctttcgat361 ccctcaatgt tcactaaata tagtttaaaa actgagatgt gaaatacaga cacaaaatgg421 aaattttaag ccaagcctac ttttccccac aaaggcattt ttgtgatacc ccaccatatt481 aatctacaga atatttgtaa ccaatatttg tgaactaatt ggatgttttg tgattntaat541 cacaata<210>23<211>469<212>DNA<213>Homo sapiens<400>231 tttttctgtg aaacacgatt aattatataa aacaaggtgc atccaaattc agtgtcagaa61 gagctttgta tttcaagggc taaggactgg accttctccc ttaaaacctc cttacccaac121 ccttgctaag gactcaggcc tggggcagag gctgtcagtc tctaaagacc ccagccctgg181 accaggacgg ggagaacaag tttggtgcat agcgtggggc tagggcagtg ggtcaggcac241 catcccttct gcaagtggcc acaggtcacc acagtggggg aaggtgggga gcttgaacct301 ccaaagatag gttcattgta ggaaaagcta gaccaatgcc ttgggtagaa acacccaaat361 ccctctacaa caactaaagc aagaatgtgg aacccatcga aatcttcccc caaaatgcca421 accataaaat agattctaca gaaataaaga aaggaatccc agcactttg<210>24<211>647<212>DNA<213>Homo sapiens<400>241 tttttttttt tttttttccg ttgagaaaag ttttatatgg aaacacatac tgatcatgaa61 cacaataaac agggagggaa gctcgggctc agccaggaaa cctgccacaa ggaagatgtt121 tggaactatc caggagtagt gtcaaacact aacaccatat ttacaagtct aatttggaac181 ctggcccttt ttaagtgcag gaggaagttg gaggagaagc catgcatgaa gtaaaaaata241 catatacaca gacataaaca cccacatttt cacacttctt ttgcccattt gttccacaag301 gaacaaatga gagaaagaag cccagcctct ctccctggag tccgtatact tactacagat361 ttgttagaga agccccagat ggggcttgga ttcaaggagc agacttcact cttactggcc421 ccactcacgt gacaattcac caagggaatg cttgggacac angcatgaat gtccggaaga481 ggcaagaaaa tatatggctc acttaactta cacattacac ataagaggcc tcagtttctc541 caagtacttt cacatttgat ctttagcaga cttctaacct gcagagacag agctcagcca601 agctgtgaga cacagaagaa agcagcattt tgatccagtg tgcactg<210>25<211>559<212>DNA<213>Homo sapiens
<400>251 tacatatgca aaataatctt gtttattaga aaatgaacat tcacaatgta aagtagaaat61 taaattacaa ataagtcttc aatggtcaaa aatggtgccc agaatttctt ctcaaaataa121 gttaatctat tcttaaacag atgcaggtaa gtagtttatc cttgtcaaaa caaaaaatta181 acagatttca ctttatgtaa gagataaggt ttatatgaac aagaagagtt tatttttaaa241 tgtaaagctg aacaagatag aatggagatg ctttgtgaag ttataaatcc cttcaaaata301 tgaaaatcat cctttcaagt ttaaatttgt tttcaaaaac atattttctc ttcttaaata361 tgtactactc ttaacaatat atcatacctt aaaatctcct gttgtcattt tctctttata421 cgcaacttgg cctactcaag aagacatcaa catggtgatt ttttttcctc aaactttatc481 tccttgctct tttcccaatc ccatgttact tcctttcgcc ttcttttgtg ccaagatgag541 caaggtataa tcctccaaa<210>26<211>243<212>DNA<213>Homo sapiens<400>261 ttttcactga atttctcttt tatttaagca ttaagaaatt ggagcatagc gacttagaaa61 actttctcaa aatgtcctta acagcaatga aaagatatca tgtgtcacgt gtaaaacctt121 ttttctgttt gttttttatt gaggctcatt gaccagggaa acataaatgt tccactcatc181 tgcttttaaa gtagagaaca aggcccacaa actctattta ataaatacaa attactaaaa241 atg<210>27<211>494<212>DNA<213>Homo sapiens<400>271 ggacgcgtgg gtccggatct ggaggaagta caggaagaag actttgtttt gcaggtggca61 gcccctccca gcatccagat aaagcgggtg atgacctacc gtgacctgga caatgacctc121 atgaagtact cagccattca gacactggat ggggagatcg acctgaaact cctcaccaaa181 gtgctcgcgc cggagcacga agtccgggag gatgatgtcg gctgggactg ggaccatctg241 ttcactgagg tgtcctcaga ggtcctcact gagtgggacc cactgcagac ggagaaggag301 gaccctgcgg ggcaggccag gcacacctga gcccgtcacc catgctctag acatgaagaa361 atgcaatgag cttaaagcta aagaagcttg taagcagctc cgaattttta cctggaatat421 tttgtaataa aaatatttat attcagtcaa ccacattgga taattcaatt gcaataaatt481 gcttattctg tgcc<210>28<211>918<212>DNA<213>Homo sapiens
<400>281 ggcggctcag cgacgcggct tctagaaccg ggtgattgaa ctaaaccttc gccgcaccga61 gtttgcagta cggccgtcac ccgcaccgct gcctgcttgc ggttggagaa atcaaggccc121 taccgggcct ccgtagtcac ctctctatag tgggcgtggc cgaggccggg gtgaccctgc181 cggagccacc gctgccagcg acatgttcaa ggtaattcag aggtccgtgg ggccagccag241 cctgagcttg ctcaccttca aagtctatgc agcaccaaaa aaggactcac ctcccaaaaa301 ttccgtgaag gttgatgagc tttcactcta ctcagttcct gagggtcaat cgaagtatgt361 ggaggaggca aggagccagc ttgaagaaag catctcacag ctccgacact attgcgagcc421 atacacaacc tggtgtcaga cagctatgac tatctccaaa atgcacctcc tggatttttt481 tccgagactt ggtgttattg gttttgctgg ccttattgga ctccttttgg ctagaggttc541 aaaaataaag aagctagtgt atccgcctgg tttcatggga ttagctgcct ccctctatta601 tccacaacaa gccatcgtgt ttgcccaggt cagtggggag agattatatg actggggttt661 acgaggatat atagtcatag aagatttgtg gaaggagaac tttcaaaagc caggaaatgt721 gaagaattca cctggaacta agtagaaaac ttcatgctct gccatcttaa tcaggaaaaa781 ctagaccaga cctctgttat cttctgtgaa atcatcctac aagcaaacta acctggaatc841 ccttcaccta gagataatgt acaagcctta gaactcctca ttctcatgtt gctatttatg901 tacctaatta aaacccaa<210>29<211>484<212>DNA<213>Homo sapiens<400>291 aacaaactag ccctcaagac gggaatagta gccaagaagc agaagacgga ggatgaggta61 ttaacaagta aaggtgacgc gtgggccaag tacatggcag aagtgaaaaa gtacaaagct121 caccagtgcg gtgacgatga taaaactcgg cccctggtga aatgacgccc ctccccacct181 gcccatggcc tgggactctc tgcgatgtac ataactattt aatgcagcgg cagcggcgac241 agccttccct gagaggactt aaaagcagaa ggaaaccgag atgcttcccg cagccgtgga301 cgattctcca ggactctttt tttaccttga gcacttgcct cgtgagactt catagaacag361 tggtttactg tccccccctt ctcacctcct cattctctct ggctctttct gtcttcctct421 tctcaccctc ctccctcccc ttagccatca cttctgggaa gtaaagaact tgacttagtg481 gccg<210>30<211>2241<212>DNA<213>Homo sapiens<400>301 agtagaaggc tcaaacaacc caaggttagt tggtctttgt gtgacagtgg gaagaagtgg61 agagaacact ctgtctcccc gacttccttc ttgaccctcc ttccgtatct ttcattcttc121 cccactggcc tttccagatg aggtccaaaa gtaagctcac tcttcaggga aaagtgatac
181 taaacccagg cgtgaggagt catccttctg ctcttacttt tccctgcatt tccttaggct241 ggcccagact ctagattcct gggcacttac ctgtatctga gacccttaga cctcctgtct301 cagtttctcc acctgtgaga tgggcagtca cacagatgag tgtgcatgag ggaatcagaa361 gcccatctgg gttgactcag aacccgggct ctccccctgc tgtgggtgga aacaaaacac421 cttttcacag aagctgttgt ccctccccca tccctgatac catcacccag aagctgggtg481 gggaatggga agctggagga gggggttaga gttagaccaa tgcgaggagc tagtaagagc541 tcgcctctcc gtgacttccc cccatgcaag ggtggcccaa ggcttcccct gatatccaaa601 gcaggacagc agccccttgg tgggattcta actgctgcta ctctgtttct ttcctcactt661 tgctttccaa ggtggtatgt gatccccagc tcaggcctgt gcagacagga aattctcccc721 tgcagcaagt aggggaggtg ggttgtggga tgtgacctcc ttccagatat caggcagtga781 gtgtaaacct gccacctcca gccctgatcc attctcacct agcggctaca ggaagctgtg841 tctgttcgat ttggtgggag gagatgtgca gggagctgta tcttgtcctc cgcttgtgaa901 aaactcaagg atgtggagaa gagtagaccg tggaaccctg ctcttctgca gccaagctga961 ggggcaggat gcgtgtggga cagtggtaga gaagcagggg ataaactcat aggctgcaac1021 aaaggtgact ctgtccctgg acactgcctc cgtactttct ccttgcttca ctggccacag1081 catctccctc cagccctcgc tatgtgcctc tgccatcttc acccatcatg gagcagaggt1141 gaggagaggc agcctgggaa tatggagacc agtgaaggac caggcctgga gagcacaggg1201 tcctacctgg gcatccagca gaggagcccc taaaggccag gagcacccca agaggaggga1261 gggcagccag cctccattga cggcgagcct ccagccctct cctactttga tcaccatttc1321 tctccaggct ttctgcctcc gagatgtggc accatagtgc ggtgccctgt ggcttcaccg1381 ccctacttcc acctccgccc agcctgtaat gtttatataa gcagcctcaa ggaccaagaa1441 ccatctgcga aaggacacac acaggaaatt cataaaagaa atctgaatgg ataaaaccat1501 gaaaaaaagt atgcttcatt agtaattaaa gaaaggcaaa tagagctgga agcatttttc1561 ccttagcaaa ccataacaga aaaaaataag acccaatatt ggcaaagaga ctactgaaaa1621 aacattccca tacattgcgt gtgggagtat acatcggtgc aggcttcctg gatgacagtt1681 gggtgatatg tgtcatgtgg cctaaaagcc tccatgtcat ttgacctacg aattctatct1741 ttgggaattt atcctaagaa aatacttaag gatttagtta gtgataagat gttcatccca1801 gcattgcaat ggagaaaaat gggaagcaat ggtttggttg ggaatttatt ccttttctgc1861 tgtaacgaaa gtttgcaata ggggattgct taagtaaatt attgtatctc catccagatg1921 gtggagtacc gcgcagacat taaaagtcat gtaaaagaac atctgactga aagaaaaatg1981 ctccttgaat attaaaaggt tgtaaaaata gtgcatgtta tgtgatttca attttgtttt2041 ttaaaatatg ggtgtatgct tgtatacgta gagcagataa aaaagacgga aggcatacta2101 aaaaatgttg agtggttatc tttgtatggt ggaacaaagt cactgtaatt ttcatctttg2161 gtttttctgt aatttccaaa ttttccacat tttgtatttc atataataaa tataatttaa2221 gaaaaaaaaa aaaaaaaaaa a<210>31<211>1599<212>DNA<213>Homo sapiens<400>311 accgggactt gcaggaagac gaggggtcgg aggacgccag cctgtatggg gagccgtcac61 caggccctga ctcgcccccg gcggagctgg cctacaggcc accactctgg gagcagagtg
121 ccaaactgtg gggcactggg ggccgggcct gggagccccc agctgaggag ctgccgcagg181 ccagcagccc accatatggg gccttggagg aggggctgga acctgaacct tccgccttct241 ccttcctctc tctgcgccgt gagctgagta ggcctgggga cctggccacc cctgaaagct301 ctgcagcggc cagcccgagg cgtattcggg cagagggggt accagcctca gcctataggt361 ctgtctcgga gcccccaggc taccaggcac acagctgcct gtcccctggc gccctgccca421 ccctctgctg cgacacctgt cgccagctgc atgctgccca ctgtgcagcc ctgcccgcct481 gccgtccagg ccactcgctg cgtgtgctgc ttggcgacgc ccagcggcgc ttgtggctac541 agcgtgcaca gatggacact ctgctctaca acagccccgg ggcccggccc taggccagct601 gggccccagg tcaagggctc ttgggagaca gctcccgtgg tgcttctctt tgctggggag661 gggtctgggc ctctggttgc cttgtccctg ggggagtcct gggcacagca gggggttgga721 gagtccaggc caacccttgc tggaggtggg actctctgta tatggaaggc tccaagggca781 gcaggcctaa gccggccagc aggagccttt ccacatttcc ccaccctcgg aacccagacg841 catgcccaca tgtgctgggt agaggggtcc agcctgttcc agccagtgcc tgaacctgtc901 cctgagaagg accctgtgcc ccaaggcccg tggctcctgg cctgctccct gcagtacgga961 gtacggggct caggacttgg gggtcctcca cgtgtcccag ggattctcca cacgagtgtc1021 ctcaagggcc tgttggtgac accgagctcc ccccacccca agctctgctg gggccaatct1081 gcggggctct gccgggtgca cggctgacct ggcttcgggg acgccggccc tccccttggg1141 tgtcacagca gctgggccca cagacaggaa atgacctgac ctcaaaccag atgtggaggt1201 gcacgagagg cgcaggaatg ggcagtggct gacagctggg aaggccctga gcgtcactgc1261 tgctgggcgg aagggcctgt ggacagccca cccaggcggc ggggtcttgg tgggtccagt1321 ggtaatggtg cccctcccct ccattccctg tgggtgcagc ccacaggctg gcgaggaggt1381 gcctctgccc taccctcggg tctggcgagg gagggggcag ggtgcatcca ctgtgacagc1441 ccctctgggc ccacaactcc ctgaccctgg aagctgagcc tgctttcctg accctccgcc1501 ccaccccctc ctccccagaa cactcagagg gacggtcaac atcagagcac attaaacgct1561 ggtgaaacca aaaaaaaaaa aaaaaaaaaa aaaaaaaaa<210>32<211>1060<212>DNA<213>Homo sapiens<400>321 ggctgccacg gggactgaag atggcgccgc gaggtgagat tccggaggta aacggttgtc61 ctccaccccg ctggaaatcc tgttctttct gaacgggtgg tataatgcta cctatttcct121 gctggaactt ttcatatttc tgtataaagg tgtcctgcta ccatatccaa cagctaacct181 agtactggat gtggtgatgc tcctccttta tcttggaatt gaagtaattc gcctgttttt241 tggtacaaag ggaaacctct gccagcgaaa gatgccactc agtattagcg tggccttgac301 cttcccatct gccatgatgg cctcctatta cctgctgctg cagacctacg tactccgcct361 ggaagccatc atgaatggca tcttgctctt cttctgtggc tcagagcttt tacttgaggt421 gctcaccttg gctgctttct ccagtatgga cacgatttga agtacagaat ttcagccagc481 agcccatcag gctgacacca cacatattgc ttctggtact ttagcccaca ccagtgagaa541 ttggtggggc aagttgtcct gagaaaggct gtgtggcttt tcttcagcac agacatttgg601 gcaagcaact cagcataagg ccagtgggta ccatcttcta aaccaggacc atcagcccaa661 gagactcttc tacactccag tatagggagg ggcaaggtta ttcccatcct gccccttctc
721 agaaccagtc ccctgctgac ctcaagttct cctccttgat caccgtggcc agagcatctc781 gtgtggacca tctaggctcc ttgggcttca agcaggacct gagccacatg ctccctgtac841 gagctgtgct atacctgtcc cacatgagca cggagagcct catgttggtg ggtttccaga901 gtgatgtgaa agcctctcac cccaatcctc ggagactgag ttccacaact tttttagtag961 ctcatagtgt tatttttcta ctctcttcat gaaactaact ttattttata ataaatatat1021 attttctgtt gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa<210>33<211>1481<212>DNA<213>Homo sapiens<400>331 ggcgtttgtg gccgtccggc tccctgacat gcagatttcc acccagaaga cagagaagga61 gccagtggtc atggaatggg ctggggtcaa agactgggtg cctgggagct gaggcagcca121 ccgtttcagc ctggccagcc ctctggaccc cgaggttgga ccctactgtg acacacctac181 catgcggaca ctcttcaacc tcctctggct tgccctggcc tgcagccctg ttcacactac241 cctgtcaaag tcagatgcca aaaaagccgc ctcaaagacg ctgctggaga agagtcagtt301 ttcagataag ccggtgcaag accggggttt ggtggtgacg gacctcaaag ctgagagtgt361 ggttcttgag catcgcagct actgctcggc aaaggcccgg gacagacact ttgctgggga421 tgtactgggc tatgtcactc catggaacag ccatggctac gatgtcacca aggtctttgg481 gagcaagttc acacagatct cacccgtctg gctgcagctg aagagacgtg gccgtgagat541 gtttgaggtc acgggcctcc acgacgtgga ccaagggtgg atgcgagctg tcaggaagca601 tgccaagggc ctgcacatag tgcctcggct cctgtttgag gactggactt acgatgattt661 ccggaacgtc ttagacagtg aggatgagat agaggagctg agcaagaccg tggtccaggt721 ggcaaagaac cagcatttcg atggcttcgt ggtggaggtc tggaaccagc tgctaagcca781 gaagcgcgtg ggcctcatcc acatgctcac ccacttggcc gaggctctgc accaggcccg841 gctgctggcc ctcctggtca tcccgcctgc catcaccccc gggaccgacc agctgggcat901 gttcacgcac aaggagtttg agcagctggc ccccgtgctg gatggtttca gcctcatgac961 ctacgactac tctacagcgc atcagcctgg ccctaatgca cccctgtcct gggttcgagc1021 ctgcgtccag gtcctggacc cgaagtccaa gtggcgaagc aaaatcctcc tggggctcaa1081 cttctatggt atggactacg cgacctccaa ggatgcccgt gagcctgttg tcggggccag1141 gtacatccag acactgaagg accacaggcc ccggatggtg tgggacagcc aggtctcaga1201 gcacttcttc gagtacaaga agagccgcag tgggaggcac gtcgtcttct acccaaccct1261 gaagtccctg caggtgcggc tggagctggc ccgggagctg ggcgttgggg tctctatctg1321 ggagctgggc cagggcctgg actacttcta cgacctgctc taggtgggca ttgcggcctc1381 cgcggtggac gtgttctttt ctaagccatg gagtgagtga gcaggtgtga aatacaggcc1441 tccactccgt ttgctgtgaa aaaaaaaaaa aaaaaaaaaa a<210>34<211>336<212>DNA<213>Homo sapiens
<400>341 gccactgcga ggccgtttta gaaaactgtt taaaacaaag agcaattgat ggataaatca61 ggaatagatt ctcttgacca tgtgacatct gatgctgtgg aacttgcaaa tcgaagtgat121 aactcttctg atagcagctt atttaaaact cagtgtatcc cttactcacc taaaggggag181 aaaagaaacc ccattcgaaa atttgttcgt acacctgaaa gtgttcacgc aagtgattca241 tcaagtgact catcttttga accaatacca ttgactataa aagctatttt tgaaagattc301 aagaacagca aaaaaaaaaa aaaaaaaaaa aaaaaa<210>35<211>1424<212>DNA<213>Homo sapiens<400>351 tagtgtagac ggaagctcct ttggtgccag attataaagg tagaaatttg tggaggacat61 cgaggagaca gaagacatgc tttatacatg tttcttttta ttttaaaaag ctttgaaata121 catttgaatg aaaaactaag cagttgctta tgctgtcagt acctgtctct gctctttaaa181 aagtaagcca ctgcctctta cctaaaggtc agcatgggct ccatcataca gcggtcaggt241 ccatcataca gcggtcaggt ccatcataca gcggtcaggt ccatcatacg gcggtcaggt301 ccatcataca gcggtcaggt ccatcatacg gcgctcaggt ccatcataca gcggtcaggt361 ccatcatacg gtggtcaggt ccatcatacg gcggtcaggt ccatcatacg gcggtcaggt421 ccatcatacg gcgctcaggt ctgtaactct tgacaggaag gtgcagtagc agaatggcga481 gatgcactga gggtaaccag atgatctcat gggacttacc cagtgttagc accaaagtcc541 catgaactga ggtgttaaaa cagaaaggcc tgtgtcctca gaacccctcc aagtcactgg601 ccaactatga ctcatgtttg gtgatgttgg atgccagtta ataaagtcca gaacttaagc661 ccaagaagag agctcagaag tgtttgtgtt actctttatt tattgaggaa taatttacag721 atagtaaaat ttacccctta tatctgtaca gttttgtgaa ttatgacaaa cttactcagg781 ttgtgtaacc accagtgcag tcaaggcata gattatttcc atccttccag agaagtctct841 gggacctttt gtagtcagtc ccctcatgga atgttttgat caacgtagaa atgcctggag901 gctgctgggt gggaggctga ccactcagcc atcagagcgt cctgtgtctc tgagccgtgc961 accccattgt ggcatggcac tggttgctgg acattcttct gcgggcccac tcacacatct1021 gcctgcctgc ggcctgtctg tgtttgtctt actcatgctc ttggagccgt agcgaatgtg1081 tctaatattc cttccacgtg gtagattgaa acttcaccaa aatagccctg tgcgcctact1141 ttgctcctga acatctctga tttctccggc tgtccctgtg tggagttctg gcagatctca1201 ccagtctaca tgacctattg gaaatgccag tgggtcattg tggcattcag acacataatt1261 caaaactgca aatggtggga ccgcactaaa gaatttttag taattaaagt acaggaggct1321 gaggtgggag gatcacttca gcccaggagg tcaggtcaag gctgcagtga gccatggttg1381 caccactaca ctgtagcctg ggtgacaaag tgagaccctg tctc<210>36<211>3174<212>DNA<213>Homo sapiens
<400>361 acagtcttcc agctccacat cctgagagga cgcttctgga gccgcgactg cccggggttg61 tgccggccgc cgctgccgcc caggccgcct cagctctcct ctgcgccggc ccgctcactc121 cgcccggccc cagccctagc gctggccgcg accccggcgc ctttgaaact tctgctggtg181 tgagtgccct cgggggttcc ccaggaatat cgatacaaca ccaacaggag atcatgaatc241 agacagataa aaatcaacaa gaaatcccat cataccttaa tgatgaacca ccagaaggtt301 caatgaaaga tcacccacag cagcagccag gcatgttgtc ccgtgtgact gggggtatct361 tcagtgttac aaagggagct gttggtgcca ccattggtgg tgtggcttgg attggtggaa421 agagtctgga agtgaccaaa acagctgtta caactgtgcc ttccatggga atagggctgg481 tgaaaggggg tgtctctgct gtggctggag gtgtacagct gttgggtctg ctgttgtaaa541 caaagtgccc ttaacaggaa agaagaaaga caaatctgac tgaaatatag agatacactt601 gcgctccaca gcactgtaat gccagtggca ttgaattgct aaattatgga ctacaaccaa661 gtcaactgtt ttggacgttt atcttctaaa ctgctgtgtt gaaagtattg atgactggct721 ttcatctaaa aagaagagac caatacgagc acagtatatg aaggtttctc atacttaagt781 tccaggtttt tatctggtaa aatgttacac ttactcggtt gtaactgaag atatggtatg841 tttgaatatt tactataagt ctttcagttt gactaaaaat gtgaaagttg aatttagtag901 atgatcttca cagttccata tgtataatgt gccaggtaac tcacctgccc cttaagaagg961 gaaccttgaa ttacataagc cgtacctttg atgtgcctaa tagtttcaga tgtcttagtt1021 ttttataacc atagttgatt aggccaagag gcattcattt cttatttaag ctggcaaaat1081 tagcaggaat tagagaagtt taaaaagata aatggtttta tgataatgtt aaccatcttt1141 tgttagtaaa tatgcgttct attattttaa tcattgatgc cttacaaaag aaaacatctt1201 ttctaatacc ctgaatatgt gctgttctta gaatcatcta tggattcttt taaaggttgt1261 ttgtgaaatt agttttccct ttttagaatc tcaggagtag tggggtaaag acatttcctg1321 ctgtcagtgg ataagacaga gcttgttaac tgttttggca gtagttaaaa tcaaattgta1381 cacttctcag cctgggttca tgcttcatca ttaatacacc tcacagtgcc taaggaacat1441 ttacttactg gtcagaaggt attttggaag agtttcatat taaggaggaa caaataataa1501 ttttaagttc ttaaaaatta cctaaaacac cccaaatata aaaagaagcc ttcacaccta1561 ttctgtcttt aggatgtctt aaattattag cagtactcct ttttttaaaa acactgtaaa1621 agtaaccaca aatatgtgag gacttactat tttaaatgga atggaatgag ctccatagat1681 tagttttgaa tataaagtat ataaaagtgc atcagtggtt tatataggct ttaaaaacat1741 gttatcttac agtcctttaa agcagccata gagtttgtat catttttcaa gccaatttca1801 gtcagggatt tgaattgttt gattatggat gataaatgtg tcatatctta ttaatatgtc1861 tcatgtctcg ttccttctta atatgattta gctggaattc attttctttt cgtttcatgt1921 ttaatttcat aaaacgttta acaattggca tatatacttg gcattcctgt ccaccaagga1981 ttgtaatcca agcctgggaa aatcttaaat ttctttttac ttaaatctgg aaatttgtct2041 ccattctgcc accttttttt tttttttttt ttatagtgga ggggaggaaa ggggggtgat2101 accttgcagt aagtaagtcg aaatagcatg cctgaaaatt tgaaacagac cattctaaca2161 cccgaggctt gtttataaaa tacttgagaa ttacattaat gtggaatcaa cagatgcaga2221 agaatataac ataactttta aaagctttca taaataccag cagcaattgt aagcaaatct2281 acaaaggttc ttgaaccttt ctattatata caaaactgaa aagtcattaa ggagttcaac2341 taatcaggaa ttaaatggtc atttatttca tgcagtatga tttaaggtat ttcttgagat2401 tctggtcaaa tgtcataatc agcaaacggg attaaaaaaa aaactccaaa atcactaaat2461 aattatctaa ataatggtat tggagaactt gtttcctgct atttggaaga gattgttgct2521 tcattgctag tttgtatttc taacttctac agttatagac tccactgtgc tttgtgtctg
2581 aatttctcag tatagacatt ttgtttactg tatgcttgca tatttatttt caactttgtt2641 tgtctttaaa attgcttgag gaaagatggt tgtaattaat ttctgctaca gaaaagccac2701 ctggtacgtt ttgtctcatc aggattgttt taaattctaa actataagtt tgttcagagg2761 ggcttttgca atgatagcag aaaactgtac aaatgtacag ttagttatag aggttcttgt2821 tgaaatgaac ttaccatctg atgatatgta tgtacagctg tgtacttgag tcttttttag2881 tttacttaga aagactagca gtttgacctg ttaaacagga ctagttcaag tcaagaaact2941 aaggttgttg tatacacctg gaggcatctg ttattcagct tatcctttga gtgggaattt3001 ggcacaatga ggataaactt atgtgaccca cttgaatggc tgatctaata atgttgacat3061 tatgcattct gtacttagtg aaatgtcaga tgaaaataac tgatgaataa tttttttgta3121 ttaaagggat gggaaaagaa cacatgaatt tgttaataaa gcactatgat ctgc<210>37<211>2076<212>DNA<213>Homo sapiens<400>371 cgcttttaaa atgattcgta ccaacgtttc tcatgtggtt ggccagttgg atgacataag61 aaaaaaccct aggaagtttg tttgcctgaa tgacaacatt gaccacaatc ataaagatgc121 tcagacagtg aaggctgttc tcagggactt ctatgaatcc atgttcccca taccttccca181 atttgaactg ccaagagagt atcgaaaccg tttccttcat atgcatgagc tgcaggaatg241 gagggcttat cgagacaaat tgaagttttg gacccattgt gtactagcaa cattgattat301 gtttactata ttctcgtttt ttgctgagca gttaattgca cttaagcgga agatatttcc361 cagaaggagg atacacaaag aagctagtcc caatcgaatc agagtataga agatcttcat421 ttgaaaacca tctacctcag catttactga gcattttaaa actcagcttc acagagatgt481 ctttgtgatg tgatgcttag cagtttggcc cgaagaagga aaatatccag taccatgctg541 ttttgtggca tgaatatagc ccactgacca ggaattattt aaccaaccca ctgaaaactt601 gtgtgttgag cagctctgaa ctgattttac ttttaaagaa tttgctcatg gacctgtcat661 cccttttata aaaaggctca ctgacaagag acagctgtta atttcccaca gcaatcattg721 cagactaact ttattaggag aagcctatgc cagctgggag tgattgctaa gaggctccag781 tctttgcatt ccaaagcctt ttgctaaagt tttgcacttt tttttttcat ttcccatttt841 taagtagtta ctaagttaac tagttattct tgcttctgag tataacgaat tgggatgtct901 aaacctattt ttatagatgt tatttaaata atgcagcaat atcacctctt attgacaata961 cctaaattat gagttttatt aatatttaag actgtaaatg gtcttaaacc actaactact1021 gaagagctca atgattgaca tctgaaatgc tttgtaatta ttgacttcag cccctaagaa1081 tgctatgatt tcacgtgcag gtctaatttc aaagggctag agttagtact acttaccaga1141 tgtaattatg ttttggaaat gtacatattc aaacagaagt gcctcatttt agaaatgagt1201 agtgctgatg gcactggcac attacagtgg tgtcttgttt aatactcatt ggtatattcc1261 agtagctatc tctctcagtt ggtttttgat agaacagaga ggccagcaaa ctttctttgt1321 aaaaggctgg ttagtaaatt attgcaggcc acctgtgtct ttgtcataca ttcttcttgc1381 tgttgtttag tttgtttttt ttcaaacaac cctctaaaaa tgtaaaaacc atgtttagct1441 tgcagctgta caaaaactgc ccaccagcca gatgtgaccc tcaggccatc atttgccaat1501 cactgagaat tagtttttgt tgttgttgtt gttgttgttt ttgagacaga gtctctctct1561 gttgcccagg ctggagtgca gtggcgcaat ctcagctcac tgcaacctcc gcctcccggg
1621 ttcaagcagt tctgtctcag ccttctgagt agctgggact acaggtgcat gccaccacac1681 cctgctaatt tttgtatttt tagtagagac gggggttcca ccatattggt caggcttatc1741 ttgaactcct gacctcaggt gatccacctg cctctgcctc ccaaagtgct gagattacag1801 gcataagcca gtgcacccag ccgagaatta gtatttttat gtatggttaa accttggcgt1861 ctagccatat tttatgtcat aatacaatgg atttgtgaag agcagattcc atgagtaact1921 ctgacaggta ttttagatca tgatctcaac aatattcttc caaaatggca tacatctttt1981 gtacaaagaa cttgaaatgt aaatactgtg tttgtgctgt aagagttgtg tatttcaaaa2041 actgaaatct cataaaaagt taaatttttg tctgac<210>38<211>2005<212>DNA<213>Homo sapiens<400>381 tgctgcagcc acaacaagtg ccaccaccag gggccttcca gctgccagtg caggctttcc61 agagccgggg actggggaac ctgcagtcat cacctcagca attctggtgg cagccaggcc121 agccagggca gcgatgatga ccgtaatgaa aagtgggatg acagctccac actggtggat181 gagttgagaa caccaggcag aggcatgtac ctggtctttg atggttcagt ggacctgcac241 taccattgca gtgcaaagtg caagagttga agcttggaaa ccttcaccta gatttaggaa301 gattcaggga aaagtctgga tgtccacgca gaagcctgct gcacgagtgg aaccctcatg361 gagaatctct accagggcag tgtggagggg aaatgtgggg ttggagcccc cacacagagt421 cccactggag cacttcctag tggagctatg agaagagaac cactgtcctc ctgacatcat481 aatggtagat ccactggcag cttgcactct cagcctgaaa aagctacaag tactcaaggc541 cagcccttga gagcatctac agatgctaaa ccctggaaag ccacaagtgt ggtgctgccc601 aaggctttgg gagcccaccc cttgtaccag cacgcccttg atgtgggata ggaggtcaaa661 ggaagttatt ttggagcttt aagatttaat gactgctctg cttggttttg gacttgtgta721 ggcttgtagc ccctttcttt tggccaattt cttcctttta gaatgggaat gttgatccaa781 tgcctatacc cccattgtat cttggaagta acaaacttgt ttcttatttt acagggtcat841 aggcagaagg gactagcctt gtctcagata agactttgga ctgtggactt ttgagttaat901 gctggaatga gttaaatatt tgcaggactg ttgagaaggc atggtagttt gtttgtttgt961 ttgtttgttt gtttgtttgt ttgttttttg agacagagtc tccctctgtc accaggctgg1021 agtgcagtgg tgcgatcgcg gctcactgca atctctgcct cctgggttca gatgattccc1081 ctgcctcggc ctccccagtg gctggggctg caggcctccg tcaccgtgcc tggctggttt1141 tttgtgtttt tgtagagatg gggtttcacc ttgttggccg ggatggtctc gatctcctga1201 ccttctgatc tgcctgcctc ggccacccaa agtgctggga ttacaggcgt gaaccactgc1261 acgcagccgg tagtattttg aaatatgaga aggacatgag atttgggaga gaccagaggt1321 ggaacaatat ggtttgaatc tgtgtccccg cccaaatctc atgttgaatt ggaatcccca1381 gtgttggagg taggacctgg tggaaagtga ttggatcatg gggcggtttc tcattaatgg1441 tttagcacta tccccttagc gctgttcttg tgagagagtt tgcatgacat ctggttgttt1501 aaaagtgtgt ggcacctccc cccacacttc ctcctgctcc agccgcgtaa gacatgcctg1561 tggccgggcg cggtggctca cgcctgtggt cccagcactt tggggggctg aggtgggcgg1621 atcacgaggt caggagatcg aggccatcgt ggctagcacg gtgaaacccc gtctctacta1681 ggaatacagg gaattggccg ggcacggtgg cgggcgcctg tggtcccggc tactcgggag
1741 gctgaggcag gagaatggcg tgggcctgga aggcggagct tgcggtgggc cgggatcgcg1801 ccactgcact tccgcctggg cgaaggagcg agactccatc tcaaaaaaaa aaaaaaaaaa1861 aaaagcaaag acgtgcctgc tacgctttca ccttccacca tcattgaaag tttcctaagg1921 cctctccaga agccgaatag ataccagcat catgcttcct gtacagcctt tagaaccatg1981 agccaattaa acctcttttc tttat<210>39<211>3055<212>DNA<213>Homo sapiens<400>391 gtttccggga tctggcgggg cctttgtctt gtgctccagc tggagctttg gtcccgtatt61 ctcggctatt tatccccagc tgcgggaggc cctggtgact ctgctgctgc agtgtctggt121 ttccctgtga cctgcaggta ctgggagtta catagctaag atgccaggac accccgaaag181 ccggaaaacg agacgcattc tcactatgtt gcccaggctg gtcttgaact ccttccctca241 agtgattctt ctgcctaatc ctcccaaagt tcttagatta ctcatgaatg gattggtgtt301 cttaccactg cagttagtgt gttctctgtt agttcatgtg aaagctggtt gttaaaaaca361 aaaacaaaca aacaaaaaac tgatattcct cttgctttgt cttttgccat gatacccctt421 tgccttttat tatgcataaa agcttcctga ggcctcatca gaagttaagc agatgttgat481 gtcatgcttg tatagccaac agaactgagg ctaataaacc tcttttctgt attaatttgc541 cagtgttaag tattcccttc tagcagcaca aaatggacta acatcccata ggttatattt601 ttattttttt cttttgtaaa tactggacaa tgatactctt atataatctt cagtataacc661 attataattg cccactataa aggagtataa tgaaaggcca tgtattttga atcacgaatg721 actatttaca aaatgtcata ctacgttttt ctttgagcct gtgacccctc tggcctgtaa781 aaacacatac atacatttag ttttgattaa catggagtta attttaagtc tgtcactcta841 aacataagtg ttagctttaa gagaattaca gagcatgtaa ttgtgtttga gtgtaggtgt901 gtactttttt gagaagaaaa caaaaatatt ggaacaaaat ataatttaaa aagtattggt961 aatttactag caaactagga atctcaaaga ttctgaaagt aaatatttat tctctgcttt1021 gtattgaatt tacttctgta aaatcttatg gctgctggct cagaatcttc tcatgcaaat1081 cctgtttttt tcttgtctgt tgatcatact aaacctaatg tatagttttc tcattccaaa1141 gttcatgaaa ttttctctat attcctgaga aattctaaga ataactttca taaaatgtat1201 tgatgttcac aagataattt taagatgtaa ttctacaata tgtgtgttac tacattttat1261 ttaattagta tattttattt ttgtatttca attggagaac cctattttgg ccaattttta1321 cttggttttt gtttccattt taatatttta cataattgaa tttttttcac tttattgagc1381 cattttgttt agatgtacac tgggaaggct tcataattca caaagttgtt ttgacatata1441 aatgaggtga acaaacacag gaaagtgcta ggtttcctgg gggtataata gatgctctat1501 aattagcagt aaatattctt gtttaagtca ctttttggct acaagtaaga gattagaaat1561 attcagcata aagagatggc attaactctg catgtaaaga aaacacatgt atacccaggc1621 tgtacaactg cctctgaaat taagaggaat tgtgtttatt tcatagttat ctctggtact1681 tcactgttat tttattttca cccccaacta tgcgtcaatc atagcccttt cccttctttg1741 cttattatgg ctacagattt ctcactgttc tctgcagccc atgtaatttc atacaatatt1801 ttgtaggttg tgataaggaa tttgaaattt ttaatatagt gaaaaattgg atttaactgg1861 agagtttgag gacattttta agttagaaat gcaccatttg aatcatttaa ttggcataat
1921 tgggatgtac tctacacagt ttataggatt aaattactga gttagtaatg tttgtatgaa1981 agaaatgtta ttagattcta ccacaaaata tagtaaacat gaaatcagtt agaataggta2041 aagcattaat aatggtaggc tatatatcat aatcactata taaagaaaca tcagaatttg2101 gaaacccctt aagcaaaaat atatcttaga aatcttaagg acacttaatg gcaagcaaaa2161 aagtttaaat ttagtttttt ataaattaca tatagcacaa tttatgtgta catgtagaat2221 cttttagttt ttagtgttta tgtaatatat tagacaataa taaacattta catttatgtt2281 tacatttata tttaattttt caaatttaat ttaaatttct aaatgcttct gtggatttaa2341 attttggaat aatcttttct tccccattga tactggaatc ttgggaagtt tcttactcat2401 atcacaatat gttttattta aatggatgca gcttccattg cagagtttat ataaataatc2461 acataccaag ccaattcctt agtcattttc tgtattaaaa aactcagaga tctagagagc2521 ttttggattg aaatatttcc ttggtgattt tggatgcaaa cctgatttct gtgttcacac2581 catggccaga gatgggactg ttagcaccac ctgcttcttt aatgtcgtcc atatgatcag2641 catcagcacc agaaactcca ggtgtcccaa acttaaagtg aaagccatag agtcctttgg2701 tgactcccat gttctgtgct ggttctagaa catgctggta aatatcagag tttctatgct2761 tatggctgat aagtagacat ttttaaaaat cataaaaact gtattttcta tatagttcag2821 ctaagtttat gaaaaagatg cagactcagt atttggagca ttgctatcgc tccatgatgt2881 gtttaaaaat tatttttaaa ttgacaaaat tttatatcta catatatata tatcttgtaa2941 cacatgatgt ttgaaatgct taattctagc caattaaaaa tgctttacct caatagttat3001 ttttattgtg agaaaggtca acatggcctt ggcccttaaa aaaaaaaaaa aaaaa<210>40<211>1909<212>DNA<213>Homo sapiens<400>401 tctaagatga cctctactgg gatgacaggg gtgagttgac aaagcttcgc acatcattca61 tcctccagca gtctcaccca ggcatgattt catgggagtg gcagaggagc gagggtgctt121 ttgcagaaag gtacaagagc tttttcaggt cttggcttgc atcacatcct tatccaatga181 accaaagcaa gtgacatggc tgagagtccc tgtaggaggt gcagggagtg tggggactct241 acaaggtgac atgtcaaaag gcaggaatac agggaacagt aaagcactgg gactgttatt301 acaaccagta acttgtttct agaagtctgc ttgatgaaac cttcaagcca catggaggag361 tcctcaaaga tgtgcttgag atattcatat aatttcttct gctctaagaa ccatgaggtg421 aacttgacct caaagtgaga ccttgacatt ggggcattta agtcagtatc tgtgtcttcc481 accccacacc tgatccctga cccaaagtct ccacagagac taatagaacc catcattaga541 ggaatgggga cctactgcac tcttcgtagt gccagggttg gatgtgttgc ttctcctaag601 ctgtttccct gcagggcagc acagcactgc ctcggaactc aaggttagcc tcgtgcctct661 cggccgaatc tgttactcaa ggattcctga aaggcagtct gcctagaaat ggaggtcaaa721 tgaaagaaag gcctaagggt tttttttgtc ttgttttgtt ttgggttttt tttgagacag781 agtctcgctc tgtcaccagg ctggagtgca gtggcacgat cttggctcac tgcaacctct841 gcctcccagg ttcaagcgat tctcctgcct cagcctccca aagtgctggg actgcaggca901 tgagccactg tgcctgacct tacagcagta ttttttaaaa atcaaaatta atgcaaaaat961 ccatgatgag gccaggcttg gtggctcatg cctgtggttc cagcactttg ggaggatccc1021 ttgagcccag gtgtttgaga ccagtttggg taacatggga agagcttgtc tctacaaaca
1081 aaaatttaaa aacaatgagc tgggcatgga actcacacct ctagccccag ctactcaggc1141 tgaggtggga ggatggcttg agcccaggag ttcagggatg cagtgtgctt tgattgcact1201 actgcgcttc accctgggca acagagtgag accccatctc tttaaaaaaa aaaaaaaatc1261 catgatgaac aaaacaagta tttgtttgag acggggtctc gctctgtcgc ccgggctgga1321 gtgcagtggt gccatctcag ctcactgcaa cctccgcctt ctgggttcaa gcgactctcc1381 tgcctcagcc tcccgagtag ctgggattac aggagcctgg cactatgcct ggctaatttt1441 tgtattttta ttggagacac catgttggtc gtggctggtc tccaactcct gacctcgggt1501 gatccgcgcc ccgcggcctc cgaaggtgct gggattgcag gcgtgagcca ccgtgcccgg1561 cctaaataaa attttgaaga ggctagaacc ccgcacttgt gccttgagct tactgacctc1621 aacaccctgg ttccactaaa actttattta caaaattatg ctgccggtct gcaggatgta1681 gaaaacaatt tgattatttt ggatattaaa atatttttta agtcttgaaa atattgttta1741 ctattaccaa agtttttgaa ccttcttaaa ttctgtacca gaggtgagtg ccacacccta1801 acctagtctg agtttttatg tgtaaagatc ataaaacgtt gtaagttttc taaaacacat1861 aagctctcaa taaacgttag cttatcattg tcaaaaaaaa aaaaaaaaa
權利要求
1.一類在人類肝臟中表達的相關基因，其特征在于，它包括(a)SEQ ID No.1～SEQ ID No.40所示的序列；(b)SEQ ID No.1～SEQ ID No.40所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.40所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
2.如權利要求1所述的相關基因，其特征在于，所述序列包括具有SEQ ID No.1～SEQ ID No.40所示的序列。
3.一種探針分子，其特征在于，所述的探針分子含有權利要求1中所述的序列中約8-100個連續(xù)的核苷酸。
全文摘要
本發(fā)明公開了一類在人類肝臟中表達的相關基因序列，它包括SEQ ID No.1～SEQ ID No.40所示的序列；SEQ ID No.1～SEQ ID No.40所示的序列中每條序列的互補序列；與SEQ ID No.1～SEQ ID No.40所示的序列中每條序列有至少70％同源性的序列；上述三種序列中一條或數(shù)條的組合。利用本發(fā)明的人類肝臟相關基因，可為研究肝臟疾病的致病機理以及開發(fā)治療肝臟疾病的藥物發(fā)揮重要作用。
文檔編號C12Q1/68GK1970762SQ20051011059
公開日2007年5月30日 申請日期2005年11月22日 優(yōu)先權日2005年11月22日
發(fā)明者黃健, 韓澤廣 申請人:上海人類基因組研究中心